ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactV2756    suspect: LH   S     KOG4526  Function unknown                                               Predicted membrane protein

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactV2756 959536  960198 221  
         (221 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YGR147c [S] KOG4526 Predicted membrane protein                        186   2e-47
SPBC106.07c [S] KOG4526 Predicted membrane protein                     78   1e-14

>YGR147c [S] KOG4526 Predicted membrane protein
          Length = 288

 Score =  186 bits (473), Expect = 2e-47
 Identities = 102/200 (51%), Positives = 133/200 (66%), Gaps = 16/200 (8%)

Query: 38  EPTGLKKLIKQYGWSALGVYLGLSCIDLPICFLAVHSLGEETIKVYINRVKQLANYGK-D 96
           EP G+K L+ +YG+SAL VY+ L+C+DLP+CFL VHSLGEE IK+Y+NR KQL   G+ D
Sbjct: 89  EPHGIKGLMAKYGYSALIVYILLTCVDLPLCFLGVHSLGEEKIKIYLNRGKQLIGMGEPD 148

Query: 97  EQELRNEIREKQ------NREELDRQSGAQDKSV---WENFKESTLLTEILIAYGIHKSL 147
           E ++  ++R KQ        E  D+   A  K+    W+  K+STLL E+LIAYGIHKSL
Sbjct: 149 ESKVIQDVRRKQAHREAVQAENADKVEDASRKTFNERWQEMKDSTLLAELLIAYGIHKSL 208

Query: 148 IFIRVPITAAITPSVVKVLRRFGFS-ADKFNKGFKTMADGAKIRNKTGKPDDFIKNGS-- 204
           I +RVP+TA +TPS VK+L+RFG     K  K F+TMA GAKIR K   P DFIKN    
Sbjct: 209 IIVRVPLTAVLTPSFVKLLQRFGIDLMKKQKKVFQTMASGAKIRYKGNNPSDFIKNEGTA 268

Query: 205 ---VPKSEFSKGKKWFNGMM 221
                +   +KG+KWF+G+M
Sbjct: 269 LDITKRKPRTKGQKWFDGLM 288


>SPBC106.07c [S] KOG4526 Predicted membrane protein
          Length = 167

 Score = 77.8 bits (190), Expect = 1e-14
 Identities = 46/126 (36%), Positives = 68/126 (53%), Gaps = 18/126 (14%)

Query: 42  LKKLIKQYGWSALGVYLGLSCIDLPICFLAVHSLGEETIKVYINRVKQLANYGKDEQELR 101
           +K+L K+YGW +LGVY+G+S +D    F+ V +LG E I             G  E  + 
Sbjct: 54  VKELTKKYGWWSLGVYIGISVLDFSASFVLVRTLGAERI-------------GYLEHSIL 100

Query: 102 NEIREKQNREELDRQSGAQDKSVWENFKESTLLTEILIAYGIHKSLIFIRVPITAAITPS 161
           N IR   N E  +  +  + ++       S++ TE+  AYGIHK+L+  RVP+TAAI P 
Sbjct: 101 NSIRRYFNWEIPESTASGEPEAY-----HSSIWTELAFAYGIHKALVVARVPLTAAIVPP 155

Query: 162 VVKVLR 167
           + K  R
Sbjct: 156 LAKRFR 161


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.319    0.138    0.402 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,476,417
Number of Sequences: 60738
Number of extensions: 568903
Number of successful extensions: 1994
Number of sequences better than 1.0e-05: 2
Number of HSP's better than  0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 1989
Number of HSP's gapped (non-prelim): 2
length of query: 221
length of database: 30,389,216
effective HSP length: 102
effective length of query: 119
effective length of database: 24,193,940
effective search space: 2879078860
effective search space used: 2879078860
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)