ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV2761 good Q KOG1599 Secondary metabolites biosynthesis, transport and catabolism Uricase (urate oxidase)
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV2761 961330 960407 -308
(308 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
SPCC1223.09 [Q] KOG1599 Uricase (urate oxidase) 213 3e-55
7297292 [Q] KOG1599 Uricase (urate oxidase) 129 4e-30
At2g26230 [Q] KOG1599 Uricase (urate oxidase) 125 1e-28
Hs17435025 [Q] KOG1599 Uricase (urate oxidase) 51 2e-06
>SPCC1223.09 [Q] KOG1599 Uricase (urate oxidase)
Length = 296
Score = 213 bits (542), Expect = 3e-55
Identities = 129/305 (42%), Positives = 169/305 (55%), Gaps = 12/305 (3%)
Query: 1 MSTYSYLADSTYGKDNVKFLKVKKSKTDPKVHEVMECNVQVLLKGDFEVSYTEADNTPIV 60
MS +Y+ YGK V+F+K K K H V E +VQ LL G+ E SYT+ADN+ +V
Sbjct: 1 MSETTYVKQCAYGKTLVRFMK-KDICPKTKTHTVYEMDVQSLLTGELEESYTKADNSIVV 59
Query: 61 PTDTVKNTILVHARKFDTWPIEKFAANLALHFTGKYAHVSGITVKIIQDRWIKYDVDNKE 120
PTDT KNTI V A+ D E FAA LA HF KY H+ G + I W + +V K
Sbjct: 60 PTDTQKNTIYVFAKNNDVSVPEVFAAKLAKHFVDKYKHIHGAALDITITPWTRMEVQGKP 119
Query: 121 SYHSFIHQGPEKKICFLDYDKKNGSSSYTLVTSIKDLTVLKSTGSMFYGYNVCEYTTLKP 180
HSFI E + + + + G + +V+S+KD+ VLKSTGS F ++ CE+TTL
Sbjct: 120 HSHSFIRNPGETRKTHVVFSEGKG---FDVVSSLKDVLVLKSTGSGFTNFHKCEFTTLPE 176
Query: 181 TTDRILSTDIFASLTWNSAKLGSLETLEAGKKDDIFNQYYASARDITLDIFAKENSPSVQ 240
TDRI ST I + T+ +T E D FN Y ++ITL+ FA ++S SVQ
Sbjct: 177 VTDRIFSTSIDCNYTFK-----HFDTFEELAGFD-FNSIYEKVKEITLETFALDDSESVQ 230
Query: 241 ATMYNMATAILKVAPEVEHVSYELPNKHYILFDLKWFK--GLDNNNELFYPSPDPNGLIK 298
ATMY MA I+ P + V Y LPNKHY +L F L +N L+ P P+G I
Sbjct: 231 ATMYKMADTIINTYPAINEVYYALPNKHYFEINLAPFNIDNLGSNCSLYQPQAYPSGYIT 290
Query: 299 CTVGR 303
CTV R
Sbjct: 291 CTVAR 295
>7297292 [Q] KOG1599 Uricase (urate oxidase)
Length = 352
Score = 129 bits (325), Expect = 4e-30
Identities = 107/333 (32%), Positives = 154/333 (46%), Gaps = 50/333 (15%)
Query: 4 YSY-LADSTYGKDNVKFLKVKKSKTDPKVHEVMECNVQVLLKGDFEVSYTEADNTPIVPT 62
Y Y + D YGKD VK L V S+ P VH + E V LK + Y + +N+ IV T
Sbjct: 30 YQYEITDHGYGKDAVKVLHV--SRNGP-VHAIQEFEVGTHLKLYSKKDYYQGNNSDIVAT 86
Query: 63 DTVKNTILVHARKFDTWPIEKFAANLALHFTGKYAHVSGITVKIIQDRWIKY-------- 114
D+ KNT+ + A+K EKFA LA HF KY+HV V + W +
Sbjct: 87 DSQKNTVYLLAKKHGIESPEKFALLLARHFINKYSHVEEAHVHVEAYPWQRVCQEETRTN 146
Query: 115 ----------------DVDNKESY-HSFIHQGPEKKICFLDYDKKNGSSSYTLVTSIKDL 157
+DN+ + H+FI C D + T++T IK L
Sbjct: 147 VNGKCENGVQGNCDFSSIDNRSLHNHAFIFTPTALHYC--DVVIRRTDPKQTVITGIKGL 204
Query: 158 TVLKSTGSMFYGYNVCEYTTLKPTTDRILSTDIFASLTWNSAKLGSLETLEAGKKDDIFN 217
VLK+T S F + E+ +L DRI ST + S W + +L+ L A
Sbjct: 205 RVLKTTQSSFVNFVNDEFRSLPDQYDRIFSTVVDCS--WEYSDTENLDFLRA-------- 254
Query: 218 QYYASARDITLDIFAKE-----NSPSVQATMYNMATAILKVAPEVEHVSYELPNKHYILF 272
+ + ++I + FA + +SPSVQ T+Y +L V P+V +S +PNKHY F
Sbjct: 255 --WQTVKNIIIRNFAGDPQVGVSSPSVQHTLYLSERQVLDVLPQVSVISMTMPNKHYFNF 312
Query: 273 DLKWFKGL--DNNNELFYPSPDPNGLIKCTVGR 303
D K F+ + +NNE+F P P+G I + R
Sbjct: 313 DTKPFQKIAPGDNNEVFIPVDKPHGTIYAQLAR 345
>At2g26230 [Q] KOG1599 Uricase (urate oxidase)
Length = 309
Score = 125 bits (313), Expect = 1e-28
Identities = 92/309 (29%), Positives = 153/309 (48%), Gaps = 19/309 (6%)
Query: 9 DSTYGKDNVKFLKVKKSKTDPKVHEVMECNVQVLLKGDFEVSYTEADNTPIVPTDTVKNT 68
D +GK V+ +V + D H +E NV + L SY DN+ IV TDT+KNT
Sbjct: 11 DQRHGKARVRVGRVWRHAHDGS-HHFVEWNVSISLLSHCLSSYRLDDNSDIVATDTIKNT 69
Query: 69 ILVHARKF-DTWPIEKFAANLALHFTGKYAHVSGITVKIIQDRWIKYDVDNKESYHSFIH 127
+ V A++ D +E+FA + HF Y V V II+ W + +D K H F
Sbjct: 70 VYVKAKECGDRLSVEEFAILIGKHFCSFYPQVFTAIVNIIEKPWERVSIDGKPHLHGF-K 128
Query: 128 QGPEKKICFLDYDKKNGSSSYTLVTSIKDLTVLKSTGSMFYGYNVCEYTTLKPTTDRILS 187
G E +K S + L + I L +LK+T S F + +YT L T +R+L+
Sbjct: 129 LGSENHTTEARVEK---SGALNLTSGIGGLALLKTTQSGFERFVRDKYTILPETRERMLA 185
Query: 188 TDIFASLTWNSAKLGSLETLEAGKKDDIFNQYYASARDITLDIF-----AKENSPSVQAT 242
T++ AS ++ + S+ T K F++ + + + +D F SPSVQ T
Sbjct: 186 TEVNASWRYSYESVASIPT-----KGLYFSEKFMDVKKVLMDTFFGPPETGVYSPSVQRT 240
Query: 243 MYNMATAILKVAPEVEHVSYELPNKHYILFDLKWFKG---LDNNNELFYPSPDPNGLIKC 299
+Y M +A+LK +V + ++PN H++ +L + + ++++ P+ +P+G I+
Sbjct: 241 LYLMGSAVLKRFADVSSIHLKMPNIHFLPVNLSTKENPSMVKFKDDVYLPTDEPHGSIEA 300
Query: 300 TVGRNQAKL 308
T+ R +KL
Sbjct: 301 TLSRITSKL 309
>Hs17435025 [Q] KOG1599 Uricase (urate oxidase)
Length = 150
Score = 50.8 bits (120), Expect = 2e-06
Identities = 45/152 (29%), Positives = 64/152 (41%), Gaps = 26/152 (17%)
Query: 152 TSIKDLTVLKSTGSMFYGYNVCEYTTLKPTTDRILSTDIFASLTWNSAKLGSLETLEAGK 211
+ IKDL VLK+T S F G+ ++TTL D+ TW++ +
Sbjct: 19 SGIKDLKVLKTTQSGFEGFIKDQFTTLPE------CRDVDFKATWDTIR----------- 61
Query: 212 KDDIFNQYYASARDITLDIFAKENSPSVQATMYNMATAILKVAPEVEHVSYELPNKHYIL 271
D+ + A D E SVQ T+ ++ L P +E + LPN HY
Sbjct: 62 --DLVMEKSAGPYD------KGEYLTSVQKTLCDIQVLSLSRVPAIEDMEISLPNIHYFN 113
Query: 272 FDLKWFKGLDNNNELFYPSPDPNGLIKCTVGR 303
D+ GL N E+ P +P G I TV R
Sbjct: 114 IDMSKM-GLINKEEVLLPLDNPYGKITGTVKR 144
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.316 0.133 0.392
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 19,477,017
Number of Sequences: 60738
Number of extensions: 838136
Number of successful extensions: 1861
Number of sequences better than 1.0e-05: 4
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 1848
Number of HSP's gapped (non-prelim): 4
length of query: 308
length of database: 30,389,216
effective HSP length: 106
effective length of query: 202
effective length of database: 23,950,988
effective search space: 4838099576
effective search space used: 4838099576
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)