ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV3179 good H KOG4230 Coenzyme transport and metabolism C1-tetrahydrofolate synthase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV3179 1103196 1100284 -971
(971 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YBR084w [H] KOG4230 C1-tetrahydrofolate synthase 1382 0.0
YGR204w [H] KOG4230 C1-tetrahydrofolate synthase 1265 0.0
SPBC839.16 [H] KOG4230 C1-tetrahydrofolate synthase 1064 0.0
Hs13699868 [H] KOG4230 C1-tetrahydrofolate synthase 972 0.0
SPBC2G2.08 [H] KOG4230 C1-tetrahydrofolate synthase 956 0.0
Hs22055576 [H] KOG4230 C1-tetrahydrofolate synthase 536 e-152
At3g12290 [H] KOG0089 Methylenetetrahydrofolate dehydrogenase/me... 292 2e-78
At4g00620 [H] KOG0089 Methylenetetrahydrofolate dehydrogenase/me... 287 6e-77
At2g38660 [H] KOG0089 Methylenetetrahydrofolate dehydrogenase/me... 282 2e-75
At4g00600 [H] KOG0089 Methylenetetrahydrofolate dehydrogenase/me... 237 6e-62
Hs5729935 [H] KOG0089 Methylenetetrahydrofolate dehydrogenase/me... 229 2e-59
CE25047 [H] KOG4230 C1-tetrahydrofolate synthase 216 2e-55
7299133 [H] KOG0089 Methylenetetrahydrofolate dehydrogenase/meth... 214 4e-55
Hs22053503 [H] KOG4230 C1-tetrahydrofolate synthase 207 5e-53
Hs13376513_2 [H] KOG0089 Methylenetetrahydrofolate dehydrogenase... 163 1e-39
Hs22040978 [H] KOG0089 Methylenetetrahydrofolate dehydrogenase/m... 139 3e-32
Hs20541728 [H] KOG0089 Methylenetetrahydrofolate dehydrogenase/m... 89 3e-17
YKR080w [H] KOG0089 Methylenetetrahydrofolate dehydrogenase/meth... 84 1e-15
Hs20542451 [H] KOG0089 Methylenetetrahydrofolate dehydrogenase/m... 82 4e-15
SPBC1711.04 [H] KOG0089 Methylenetetrahydrofolate dehydrogenase/... 76 3e-13
Hs17437830 [H] KOG0089 Methylenetetrahydrofolate dehydrogenase/m... 55 5e-07
>YBR084w [H] KOG4230 C1-tetrahydrofolate synthase
Length = 975
Score = 1382 bits (3577), Expect = 0.0
Identities = 682/948 (71%), Positives = 800/948 (83%), Gaps = 6/948 (0%)
Query: 30 PTCH---YHLLSGTKIAKSIREEAFKEIKQIQIKSPSFEPCLKIIQVGTRPDSTAYVRMK 86
PT H YH+LSG K+A+SIRE+A EI+ I++K P+F+P LKIIQVG RPDS+ YVRMK
Sbjct: 28 PTVHASQYHILSGRKLAQSIREKANDEIQAIKLKHPNFKPTLKIIQVGARPDSSTYVRMK 87
Query: 87 LKASEESGVKCIVEKLPEDIDELALLQKIQQVNDDDSIHGLLIQLPLPRHLNEAEVTNAV 146
LKAS++S V CI+EKLP +I E+ LL+KI +NDDDSIHGLLIQLPLPRHL+E +TNAV
Sbjct: 88 LKASKDSNVDCIIEKLPAEITEVELLKKISDINDDDSIHGLLIQLPLPRHLDETTITNAV 147
Query: 147 SYQKDVDGFHRFNVGELSKRGGKPYFIPCTPNGCIRLLKESGIDLRGKRAVVIGRSDIVG 206
++KDVDGFHR+N GEL+K+GGKPYFIPCTP GC++LL+E+ + L GK AVV+GRS IVG
Sbjct: 148 DFKKDVDGFHRYNAGELAKKGGKPYFIPCTPYGCMKLLEEAHVKLDGKNAVVLGRSSIVG 207
Query: 207 TPVASLLKDLNATVTICHRYTENIADVVKNADVVIAACGVPNMVKPNWLKQDAIVIDVGI 266
P+ASLLK+ NATVT+CH +T NIA+VV AD+VIAACG+P VK +W+K+ A+VIDVGI
Sbjct: 208 NPIASLLKNANATVTVCHSHTRNIAEVVSQADIVIAACGIPQYVKSDWIKEGAVVIDVGI 267
Query: 267 NYVPDASKKSGRKLVGDVDFELCKDKASYITPVPGGVGPMTVAMLVQNVLLAAKRQREES 326
NYVPD SKKSG+KLVGDVDF+ K+K SYITPVPGGVGPMTVAMLV NVLLAAKRQ ES
Sbjct: 268 NYVPDISKKSGQKLVGDVDFDSVKEKTSYITPVPGGVGPMTVAMLVSNVLLAAKRQFVES 327
Query: 327 TRMPHIKPLPLKIEAKVPSDIEISRKQKPKYIEAVANELGVHPSELELYGHYKAKVSPKI 386
++P IKPLPL +E+ VPSDI+ISR Q PK+I+ VA ELG+H ELELYGHYKAK+SP I
Sbjct: 328 EKLPVIKPLPLHLESPVPSDIDISRAQSPKHIKQVAEELGIHSHELELYGHYKAKISPNI 387
Query: 387 ANRLADNENGKYVLVAGITPTPLGEGKSTTTIGLVQALSAHLHKPSIANVRQPSMGPTFG 446
RL ENGKYVLVAGITPTPLGEGKSTTT+GLVQALSAHL KPSIANVRQPS+GPT G
Sbjct: 388 FKRLESRENGKYVLVAGITPTPLGEGKSTTTMGLVQALSAHLGKPSIANVRQPSLGPTLG 447
Query: 447 VKXXXXXXXYAQVIPMDEFNMHLTGDIHXXXXXXXXXXXXXDTRMFHEATQKKHETFYKR 506
VK YAQVIPMDEFN+HLTGDIH DTRMFHEATQK TFYKR
Sbjct: 448 VKGGAAGGGYAQVIPMDEFNLHLTGDIHAISAANNLLAAAIDTRMFHEATQKNDSTFYKR 507
Query: 507 LVPVKKGVRSFTPSMLTRLNKLGINKTNPDDLNTEEIIKFARLNIDPDTITIKRVVDVND 566
LVP KKG+R FTPSM RL +L I K +PD L EE+ +FARLNI+PDTITI+RVVD+ND
Sbjct: 508 LVPRKKGIRKFTPSMQRRLKRLDIEKEDPDALTPEEVKRFARLNINPDTITIRRVVDIND 567
Query: 567 RMLRQITIGQAPTEKGFTRQTGFDITVASELMAILALSKDLKDLRERVGRIVIGSNYEKE 626
RMLRQITIG+A TEKGFTR TGFDITVASELMAILALSK L +++ER+GR+VIG++Y+ +
Sbjct: 568 RMLRQITIGEAATEKGFTRTTGFDITVASELMAILALSKSLHEMKERIGRMVIGADYDNK 627
Query: 627 PITVNDIGCAGAIAALLKDAVKPNLMQTLEGTPVLVHAGPFANISIGASSVIADRVALKL 686
P+TV DIGC GA+ ALL+DA+KPNLMQTLEGTPV+VHAGPFANISIGASSVIAD +ALKL
Sbjct: 628 PVTVEDIGCTGALTALLRDAIKPNLMQTLEGTPVMVHAGPFANISIGASSVIADLMALKL 687
Query: 687 VGKPKEA--DESA-ESGYVVTEAGFDFTMGGERFFNIKCRASGLTPNAVVLVATVRALKL 743
VG K D++ E GYVVTEAGFDF MGGERFF+IKCR+SGL P+AVVLVATVRALK
Sbjct: 688 VGSEKNPLNDKNIHEPGYVVTEAGFDFAMGGERFFDIKCRSSGLVPDAVVLVATVRALKS 747
Query: 744 HGGAPDVKPGQSLPEEYLKENVDLVRNGTANMCKQIENIKQFGVPVVVAINKFESDTEAE 803
HGGAP+VKPGQSLP+EY +EN+D V G +N+ KQIENIK FG+PVVVAIN+FE+D++AE
Sbjct: 748 HGGAPNVKPGQSLPKEYTEENIDFVAKGVSNLVKQIENIKTFGIPVVVAINRFETDSQAE 807
Query: 804 IDAIKEAAQRVGAFDVVSTNHWAEGGKGAIKLAEAVIEASKQPSEFKFLYDVNDTVENKL 863
I+ IK+AA GA V++NHW EGGKGA++LA AV++A+K+P F FLYDVN ++E+KL
Sbjct: 808 IEVIKKAALNAGASHAVTSNHWMEGGKGAVELAHAVVDATKEPKNFNFLYDVNSSIEDKL 867
Query: 864 STIVQKMYGGANIEISEEAQKKIDMYRQQGFGDLPICIAKTQYSLSHDAKLKNVPTGFTF 923
++IVQKMYGGA IE+S EAQKKID Y++QGFG+LPICIAKTQYSLSHD LK VP GFTF
Sbjct: 868 TSIVQKMYGGAKIEVSPEAQKKIDTYKKQGFGNLPICIAKTQYSLSHDPSLKGVPRGFTF 927
Query: 924 PIRDVRASIGAGYLYALAAEIQTIPGLSTHAGYMNVEIDEDGEIEGLF 971
PIRDVRASIGAGYLYALAAEIQTIPGLST+AGYM VE+D+DGEIEGLF
Sbjct: 928 PIRDVRASIGAGYLYALAAEIQTIPGLSTYAGYMAVEVDDDGEIEGLF 975
>YGR204w [H] KOG4230 C1-tetrahydrofolate synthase
Length = 946
Score = 1265 bits (3274), Expect = 0.0
Identities = 640/944 (67%), Positives = 740/944 (77%), Gaps = 10/944 (1%)
Query: 36 LLSGTKIAKSIREEAFKEIKQIQIKSPSFEPCLKIIQVGTRPDSTAYVRMKLKASEESGV 95
+L G A+ R EIK IQ P F P L IIQVG RPDS YVRMK KA+EE+G+
Sbjct: 5 VLDGKACAQQFRSNIANEIKSIQGHVPGFAPNLAIIQVGNRPDSATYVRMKRKAAEEAGI 64
Query: 96 KCIVEKLPEDIDELALLQKIQQVNDDDSIHGLLIQLPLPRHLNEAEVTNAVSYQKDVDGF 155
L E E +L+ + Q+N+D HG+++QLPLP HL+E +T+ V +KDVDGF
Sbjct: 65 VANFIHLDESATEFEVLRYVDQLNEDPHTHGIIVQLPLPAHLDEDRITSRVLAEKDVDGF 124
Query: 156 HRFNVGELSKRGGKPYFIPCTPNGCIRLLKESGIDLRGKRAVVIGRSDIVGTPVASLLKD 215
N+GEL+K+ G P+F+PCTP G I LL ++ + + G R+VVIGRSDIVG+PVA LLK
Sbjct: 125 GPTNIGELNKKNGHPFFLPCTPKGIIELLHKANVTIEGSRSVVIGRSDIVGSPVAELLKS 184
Query: 216 LNATVTICHRYTENIADVVKNADVVIAACGVPNMVKPNWLK--------QDAIVIDVGIN 267
LN+TVTI H T +IA + +AD+V+ A G P VK W K + +VIDVG N
Sbjct: 185 LNSTVTITHSKTRDIASYLHDADIVVVAIGQPEFVKGEWFKPRDGTSSDKKTVVIDVGTN 244
Query: 268 YVPDASKKSGRKLVGDVDFELCKDKASYITPVPGGVGPMTVAMLVQNVLLAAKRQREEST 327
YV D SKKSG K VGDV+F ITPVPGGVGPMTVAML+QN L+AAKRQ EES+
Sbjct: 245 YVADPSKKSGFKCVGDVEFNEAIKYVHLITPVPGGVGPMTVAMLMQNTLIAAKRQMEESS 304
Query: 328 RMPHIKPLPLKIEAKVPSDIEISRKQKPKYIEAVANELGVHPSELELYGHYKAKVSPKIA 387
+ I PLPLK+ VPSDI+ISR Q+PK I +A ELG++ ELELYGHYKAK+SPK+
Sbjct: 305 KPLQIPPLPLKLLTPVPSDIDISRAQQPKLINQLAQELGIYSHELELYGHYKAKISPKVI 364
Query: 388 NRLADNENGKYVLVAGITPTPLGEGKSTTTIGLVQALSAHLHKPSIANVRQPSMGPTFGV 447
RL +NGKY+LV+GITPTPLGEGKSTTT+GLVQAL+AHL KP+IANVRQPS+GPT GV
Sbjct: 365 ERLQTRQNGKYILVSGITPTPLGEGKSTTTMGLVQALTAHLGKPAIANVRQPSLGPTLGV 424
Query: 448 KXXXXXXXYAQVIPMDEFNMHLTGDIHXXXXXXXXXXXXXDTRMFHEATQKKHETFYKRL 507
K Y+QVIPMDEFN+HLTGDIH DTRMFHE TQK TFY RL
Sbjct: 425 KGGAAGGGYSQVIPMDEFNLHLTGDIHAIGAANNLLAAAIDTRMFHETTQKNDATFYNRL 484
Query: 508 VPVKKGVRSFTPSMLTRLNKLGINKTNPDDLNTEEIIKFARLNIDPDTITIKRVVDVNDR 567
VP K G R FTPSM RLN+LGI KTNPDDL EEI KFARLNIDPDTITIKRVVD+NDR
Sbjct: 485 VPRKNGKRKFTPSMQRRLNRLGIQKTNPDDLTPEEINKFARLNIDPDTITIKRVVDINDR 544
Query: 568 MLRQITIGQAPTEKGFTRQTGFDITVASELMAILALSKDLKDLRERVGRIVIGSNYEKEP 627
MLRQITIGQAPTEK TR TGFDITVASELMAILALSKDL+D++ER+GR+V+ ++ + P
Sbjct: 545 MLRQITIGQAPTEKNHTRVTGFDITVASELMAILALSKDLRDMKERIGRVVVAADVNRSP 604
Query: 628 ITVNDIGCAGAIAALLKDAVKPNLMQTLEGTPVLVHAGPFANISIGASSVIADRVALKLV 687
+TV D+GC GA+ ALL+DA+KPNLMQTLEGTPVLVHAGPFANISIGASSVIADRVALKLV
Sbjct: 605 VTVEDVGCTGALTALLRDAIKPNLMQTLEGTPVLVHAGPFANISIGASSVIADRVALKLV 664
Query: 688 GKPKEADESAESGYVVTEAGFDFTMGGERFFNIKCRASGLTPNAVVLVATVRALKLHGGA 747
G EA E+GYVVTEAGFDFTMGGERFFNIKCR+SGLTPNAVVLVATVRALK HGGA
Sbjct: 665 GTEPEA--KTEAGYVVTEAGFDFTMGGERFFNIKCRSSGLTPNAVVLVATVRALKSHGGA 722
Query: 748 PDVKPGQSLPEEYLKENVDLVRNGTANMCKQIENIKQFGVPVVVAINKFESDTEAEIDAI 807
PDVKPGQ LP Y +EN++ V G ANMCKQI NIKQFGVPVVVAINKFE+DTE EI AI
Sbjct: 723 PDVKPGQPLPSAYTEENIEFVEKGAANMCKQIANIKQFGVPVVVAINKFETDTEGEIAAI 782
Query: 808 KEAAQRVGAFDVVSTNHWAEGGKGAIKLAEAVIEASKQPSEFKFLYDVNDTVENKLSTIV 867
++AA GAF+ V++NHWAEGGKGAI LA+AVIEAS QP +F FLYDVN +VE+KL+TIV
Sbjct: 783 RKAALEAGAFEAVTSNHWAEGGKGAIDLAKAVIEASNQPVDFHFLYDVNSSVEDKLTTIV 842
Query: 868 QKMYGGANIEISEEAQKKIDMYRQQGFGDLPICIAKTQYSLSHDAKLKNVPTGFTFPIRD 927
QKMYGGA I+I EAQ+KIDMY++QGFG+LPICIAKTQYSLSHDA LK VPTGFTFPIRD
Sbjct: 843 QKMYGGAAIDILPEAQRKIDMYKEQGFGNLPICIAKTQYSLSHDATLKGVPTGFTFPIRD 902
Query: 928 VRASIGAGYLYALAAEIQTIPGLSTHAGYMNVEIDEDGEIEGLF 971
VR S GAGYLYALAAEIQTIPGL+T+AGYM VE+D+DGEI+GLF
Sbjct: 903 VRLSNGAGYLYALAAEIQTIPGLATYAGYMAVEVDDDGEIDGLF 946
>SPBC839.16 [H] KOG4230 C1-tetrahydrofolate synthase
Length = 937
Score = 1064 bits (2752), Expect = 0.0
Identities = 542/938 (57%), Positives = 689/938 (72%), Gaps = 8/938 (0%)
Query: 36 LLSGTKIAKSIREEAFKEIKQIQIKSPSFEPCLKIIQVGTRPDSTAYVRMKLKASEESGV 95
LL GT +A+ +REE ++I I+ P F LKIIQVG R DS YVRMK +A+ E+G+
Sbjct: 4 LLEGTSLARKVREELREQISSIKSVDPYFNVSLKIIQVGGREDSNVYVRMKTRAANEAGI 63
Query: 96 KCIVEKLPEDIDELALLQKIQQVNDDDSIHGLLIQLPLPRHLNEAEVTNAVSYQKDVDGF 155
C PEDI E LL I+ N+D ++HG+++QLPLP H+NE +T AV+ +KDVDGF
Sbjct: 64 SCEHVNFPEDITEYDLLLAIKGFNEDPTVHGIIVQLPLPAHINEQIITEAVAPEKDVDGF 123
Query: 156 HRFNVGELSKRGGKPYFIPCTPNGCIRLLKESGIDLRGKRAVVIGRSDIVGTPVASLLKD 215
N+G+L+KR G+P F CTP G + +LK GI+++GK AVVIGRS+IVG P++ LL+
Sbjct: 124 CETNLGKLTKREGQPLFTACTPKGIMCILKHYGINVQGKHAVVIGRSNIVGRPMSILLEK 183
Query: 216 LNATVTICHRYTENIADVVKNADVVIAACGVPNMVKPNWLKQDAIVIDVGINYVPDASKK 275
NATVT+CH TE+IAD+V+ AD+V+AA G+P+ VK +WLK+ + IDVGIN +PDA+KK
Sbjct: 184 ANATVTLCHSKTESIADIVRTADIVVAAIGIPHFVKADWLKKGVVAIDVGINSIPDATKK 243
Query: 276 SGRKLVGDVDFELCKDKASYITPVPGGVGPMTVAMLVQNVLLAAKRQREESTRMPHIKP- 334
SG +L GD+DFE K+ AS ITPVPG VGPMTVAML+QNV+ +A R R+ S + KP
Sbjct: 244 SGYRLTGDIDFENAKEVASAITPVPGSVGPMTVAMLLQNVVESAVRFRKMSRKR---KPT 300
Query: 335 -LPLKIEAKVPSDIEISRKQKPKYIEAVANELGVHPSELELYGHYKAKVSPKIANRLADN 393
LPLK++ VPSDIEI+R Q PK I +A+E+G+ SELE YG +KAKV+ +I RLA
Sbjct: 301 LLPLKLQTPVPSDIEIARSQTPKNIGDLASEIGIAKSELEFYGSHKAKVNLEILQRLAHR 360
Query: 394 ENGKYVLVAGITPTPLGEGKSTTTIGLVQALSAHLHKPSIANVRQPSMGPTFGVKXXXXX 453
+G YV+V GITPTP GEGKST T GLVQALS +L K +IA VRQPS GPTFG+K
Sbjct: 361 RDGHYVVVTGITPTPFGEGKSTLTAGLVQALS-NLDKLAIACVRQPSQGPTFGIKGGAAG 419
Query: 454 XXYAQVIPMDEFNMHLTGDIHXXXXXXXXXXXXXDTRMFHEATQKKHETFYKRLVPVKKG 513
Y+Q IPM+EFN+HLTGDIH DTRMFHE TQ YKRL VK
Sbjct: 420 GGYSQFIPMEEFNLHLTGDIHAITAATNLLAAAIDTRMFHENTQSD-AALYKRLTLVKGN 478
Query: 514 VRSFTPSMLTRLNKLGINKTNPDDLNTEEIIKFARLNIDPDTITIKRVVDVNDRMLRQIT 573
R F P M RL KLGI+KTNP++L EE KFARL+I+P TI+ R +DVNDR LR+IT
Sbjct: 479 KREFAPVMFRRLKKLGIDKTNPEELTEEEQRKFARLDIEPSTISWNRTLDVNDRFLRKIT 538
Query: 574 IGQAPTEKGFTRQTGFDITVASELMAILALSKDLKDLRERVGRIVIGSNYEKEPITVNDI 633
IG+ PTEKGFTRQTGFD++VASE M++LAL+ DLKD+RER+GR+V+ SN EP+T +D+
Sbjct: 539 IGENPTEKGFTRQTGFDLSVASECMSVLALATDLKDMRERLGRMVVASNKSGEPVTADDL 598
Query: 634 GCAGAIAALLKDAVKPNLMQTLEGTPVLVHAGPFANISIGASSVIADRVALKLVGKPKEA 693
G GA+ LLKDA+KP LMQTLEGTP LVHAGPFANISIGASS++ADR+ALKL G +
Sbjct: 599 GVGGALTVLLKDAIKPTLMQTLEGTPALVHAGPFANISIGASSILADRIALKLAGTEVDE 658
Query: 694 DESAESGYVVTEAGFDFTMGGERFFNIKCRASGLTPNAVVLVATVRALKLHGGAPDVKPG 753
D E+GYVVTEAGF +G E+FFNIKCR SGL P+A+V+VATV+ALKLHGG P V PG
Sbjct: 659 DAKKEAGYVVTEAGFASDIGMEKFFNIKCRTSGLKPDAIVIVATVQALKLHGGGPPVGPG 718
Query: 754 QSLPEEYLKENVDLVRNGTANMCKQIENIKQFGVPVVVAINKFESDTEAEIDAIKEAAQR 813
+ +PE Y +E+VDLVR G AN+ K I N +++G+PVVVAINKF SD+ EI AI+E A
Sbjct: 719 KPIPEVYKREDVDLVRKGCANLAKHISNARKYGLPVVVAINKFSSDSPNEISAIREEALA 778
Query: 814 VGAFDVVSTNHWAEGGKGAIKLAEAVIEASKQ-PSEFKFLYDVNDTVENKLSTIVQKMYG 872
GA D V +NHWAEGGKGA+ +A A+I A + SEF+ LYDV++ +E K+ I ++MYG
Sbjct: 779 AGATDAVDSNHWAEGGKGALGVARALINACENVDSEFRLLYDVHEPIEKKIEIIAKEMYG 838
Query: 873 GANIEISEEAQKKIDMYRQQGFGDLPICIAKTQYSLSHDAKLKNVPTGFTFPIRDVRASI 932
IE+S A+++++ + +QG+ +LPICIAKTQYSLSHD LK PT FT PIRD+R S
Sbjct: 839 ADGIELSPLAKERLETFTKQGYNNLPICIAKTQYSLSHDPDLKGAPTNFTVPIRDMRLSA 898
Query: 933 GAGYLYALAAEIQTIPGLSTHAGYMNVEIDEDGEIEGL 970
GAG++Y LAA I TIPGL T Y N++I E+G+I GL
Sbjct: 899 GAGFIYPLAAAISTIPGLPTKPAYYNIDIAENGDIVGL 936
>Hs13699868 [H] KOG4230 C1-tetrahydrofolate synthase
Length = 935
Score = 972 bits (2512), Expect = 0.0
Identities = 499/940 (53%), Positives = 657/940 (69%), Gaps = 14/940 (1%)
Query: 36 LLSGTKIAKSIREEAFKEIKQIQIKSPSFEPCLKIIQVGTRPDSTAYVRMKLKASEESGV 95
+L+G +I+ IR ++ Q++ + P F P L I+QVG R DS Y+ +KLKA+EE G+
Sbjct: 6 ILNGKEISAQIRARLKNQVTQLKEQVPGFTPRLAILQVGNRDDSNLYINVKLKAAEEIGI 65
Query: 96 KCIVEKLPEDIDELALLQKIQQVNDDDSIHGLLIQLPLPRH--LNEAEVTNAVSYQKDVD 153
K KLP E +++ I +N+D ++HG L+QLPL +N EV NA++ +KDVD
Sbjct: 66 KATHIKLPRTTTESEVMKYITSLNEDSTVHGFLVQLPLDSENSINTEEVINAIAPEKDVD 125
Query: 154 GFHRFNVGELSKRGGKPYFIPCTPNGCIRLLKESGIDLRGKRAVVIGRSDIVGTPVASLL 213
G N G L++ FIPCTP GC+ L+KE+G+ + G+ AVV+GRS IVG P+ LL
Sbjct: 126 GLTSINAGRLARGDLNDCFIPCTPKGCLELIKETGVPIAGRHAVVVGRSKIVGAPMHDLL 185
Query: 214 KDLNATVTICHRYTENIADVVKNADVVIAACGVPNMVKPNWLKQDAIVIDVGINYVPDAS 273
NATVT CH T ++ + V D+++ A G P MVK W+K AIVID GINYVPD
Sbjct: 186 LWNNATVTTCHSKTAHLDEEVNKGDILVVATGQPEMVKGEWIKPGAIVIDCGINYVPDDK 245
Query: 274 KKSGRKLVGDVDFELCKDKASYITPVPGGVGPMTVAMLVQNVLLAAKRQREE-STRMPHI 332
K +GRK+VGDV ++ K++AS+ITPVPGGVGPMTVAML+Q+ + +AKR E+ I
Sbjct: 246 KPNGRKVVGDVAYDEAKERASFITPVPGGVGPMTVAMLMQSTVESAKRFLEKFKPGKWMI 305
Query: 333 KPLPLKIEAKVPSDIEISRKQKPKYIEAVANELGVHPSELELYGHYKAKVSPKIANRLAD 392
+ L ++ VPSDI+ISR KPK I +A E+G+ E+ELYG KAKV RL
Sbjct: 306 QYNNLNLKTPVPSDIDISRSCKPKPIGKLAREIGLLSEEVELYGETKAKVLLSALERLKH 365
Query: 393 NENGKYVLVAGITPTPLGEGKSTTTIGLVQALSAHLHKPSIANVRQPSMGPTFGVKXXXX 452
+GKYV+V GITPTPLGEGKSTTTIGLVQAL AHL++ A VRQPS GPTFG+K
Sbjct: 366 RPDGKYVVVTGITPTPLGEGKSTTTIGLVQALGAHLYQNVFACVRQPSQGPTFGIKGGAA 425
Query: 453 XXXYAQVIPMDEFNMHLTGDIHXXXXXXXXXXXXXDTRMFHEATQKKHETFYKRLVPVKK 512
Y+QVIPM+EFN+HLTGDIH D R+FHE TQ + + RLVP
Sbjct: 426 GGGYSQVIPMEEFNLHLTGDIHAITAANNLVAAAIDARIFHELTQTD-KALFNRLVPSVN 484
Query: 513 GVRSFTPSMLTRLNKLGINKTNPDDLNTEEIIKFARLNIDPDTITIKRVVDVNDRMLRQI 572
GVR F+ + RL +LGI KT+P L EEI +FARL+IDP+TIT +RV+D NDR LR+I
Sbjct: 485 GVRRFSDIQIRRLKRLGIEKTDPTTLTDEEINRFARLDIDPETITWQRVLDTNDRFLRKI 544
Query: 573 TIGQAPTEKGFTRQTGFDITVASELMAILALSKDLKDLRERVGRIVIGSNYEKEPITVND 632
TIGQAPTEKG TR FDI+VASE+MA+LAL+ L+D+RER+G++V+ S+ + EP++ D
Sbjct: 545 TIGQAPTEKGHTRTAQFDISVASEIMAVLALTTSLEDMRERLGKMVVASSKKGEPVSAED 604
Query: 633 IGCAGAIAALLKDAVKPNLMQTLEGTPVLVHAGPFANISIGASSVIADRVALKLVGKPKE 692
+G +GA+ L+KDA+KPNLMQTLEGTPV VHAGPFANI+ G SS+IAD++ALKLVG
Sbjct: 605 LGVSGALTVLMKDAIKPNLMQTLEGTPVFVHAGPFANIAHGNSSIIADQIALKLVGP--- 661
Query: 693 ADESAESGYVVTEAGFDFTMGGERFFNIKCRASGLTPNAVVLVATVRALKLHGGAPDVKP 752
G+VVTEAGF +G E+FFNIKCR SGL P+ VVLVATVRALK+HGG P V
Sbjct: 662 ------EGFVVTEAGFGADIGMEKFFNIKCRYSGLCPHVVVLVATVRALKMHGGGPTVTA 715
Query: 753 GQSLPEEYLKENVDLVRNGTANMCKQIENIKQFGVPVVVAINKFESDTEAEIDAIKEAAQ 812
G LP+ Y++EN++LV G +N+ KQIEN + FG+PVVVA+N F++DTE+E+D I ++
Sbjct: 716 GLPLPKAYIQENLELVEKGFSNLKKQIENARMFGIPVVVAVNAFKTDTESELDLISRLSR 775
Query: 813 RVGAFDVVSTNHWAEGGKGAIKLAEAVIEASKQPSEFKFLYDVNDTVENKLSTIVQKMYG 872
GAFD V HWAEGGKGA+ LA+AV A++ PS F+ LYD+ VE+K+ I QK+YG
Sbjct: 776 EHGAFDAVKCTHWAEGGKGALALAQAVQRAAQAPSSFQLLYDLKLPVEDKIRIIAQKIYG 835
Query: 873 GANIEISEEAQKKIDMYRQQGFGDLPICIAKTQYSLSHDAKLKNVPTGFTFPIRDVRASI 932
+IE+ EAQ K ++Y +QGFG+LPIC+AKT SLSH+ + K VPTGF PIRD+RAS+
Sbjct: 836 ADDIELLPEAQHKAEVYTKQGFGNLPICMAKTHLSLSHNPEQKGVPTGFILPIRDIRASV 895
Query: 933 GAGYLYALAAEIQTIPGLSTHAGYMNVEID-EDGEIEGLF 971
GAG+LY L + T+PGL T + ++++D E ++ GLF
Sbjct: 896 GAGFLYPLVGTMSTMPGLPTRPCFYDIDLDPETEQVNGLF 935
>SPBC2G2.08 [H] KOG4230 C1-tetrahydrofolate synthase
Length = 969
Score = 956 bits (2470), Expect = 0.0
Identities = 496/942 (52%), Positives = 661/942 (69%), Gaps = 13/942 (1%)
Query: 35 HLLSGTKIAKSIREEAFKEIKQIQIKSPSFEPCLKIIQVGTRPDSTAYVRMKLKASEESG 94
++L+G +A+ ++ +E++ ++ K +F+P L I+QVG R DS YVRMK KA+ G
Sbjct: 34 NILNGKLLARKLQRSVAEEVQALKAKDRNFKPALAIVQVGKREDSNVYVRMKEKAARLVG 93
Query: 95 VKCIVEKLPEDIDELALLQKIQQVNDDDSIHGLLIQLPLPRHLNEAEVTNAVSYQKDVDG 154
+ PE I ALL +++++NDD ++HG+L+QLPLP+HLNE VT +++ KDVDG
Sbjct: 94 IDFKYCPFPETIQMPALLHELKKLNDDHTVHGVLVQLPLPKHLNERTVTESITPPKDVDG 153
Query: 155 FHRFNVGELSKRGGKPYFIPCTPNGCIRLLKESGIDLRGKRAVVIGRSDIVGTPVASLLK 214
F FN+G L+K P PCTP G + LLK++ I + G AVV+GRSDIVG P++ LL+
Sbjct: 154 FGAFNIGLLAKNDATPIHYPCTPKGIMELLKDNKISVAGLNAVVLGRSDIVGNPISYLLR 213
Query: 215 DLNATVTICHRYTENIADVVKNADVVIAACGVPNMVKPNWLKQDAIVIDVGINYVPDASK 274
NATVT+CH T+++ + NAD+VIAA G P V+ WLK ++V+DVGIN V +
Sbjct: 214 KDNATVTVCHSKTKDLIQHISNADLVIAALGKPEFVRGEWLKPGSVVVDVGINAV---QR 270
Query: 275 KSGRKLVGDVDFELCKDKASYITPVPGGVGPMTVAMLVQNVLLAAKRQREESTRMPHIKP 334
R LVGDV FE AS ITPVPGGVGPMTVAML++N++ AAK R E+ I
Sbjct: 271 NGKRVLVGDVHFESASKVASSITPVPGGVGPMTVAMLMENIVNAAKIARTENI-YRKIDL 329
Query: 335 LPLKIEAKVPSDIEISRKQKPKYIEAVANELGVHPSELELYGHYKAKVSPKIANRLADNE 394
PL+++ VPSDIEI+ Q+PK I +A E+G++ +ELE YG+YKAKV+ + RL +
Sbjct: 330 NPLELKKPVPSDIEIANSQEPKLISNLAKEMGIYDTELENYGNYKAKVNLAVYERLKHRK 389
Query: 395 NGKYVLVAGITPTPLGEGKSTTTIGLVQALSAHLHKPSIANVRQPSMGPTFGVKXXXXXX 454
+G YV+V+GITPTP GEGKST GLVQA+ HL K IA VRQPS GPTFGVK
Sbjct: 390 DGNYVVVSGITPTPFGEGKSTVVAGLVQAM-GHLGKLGIACVRQPSQGPTFGVKGGAAGG 448
Query: 455 XYAQVIPMDEFNMHLTGDIHXXXXXXXXXXXXXDTRMFHEATQKKHETFYKRLVPVKKGV 514
YAQ IPMD+FN+H+TGDIH +TRMFHE TQ KRL+PVK G
Sbjct: 449 GYAQFIPMDDFNLHMTGDIHAVTAANNLLVAALETRMFHENTQ-SDAALIKRLIPVKNGR 507
Query: 515 RSFTPSMLTRLNKL-GINKTNPDDLN--TEEIIK-FARLNIDPDTITIKRVVDVNDRMLR 570
R ++ R N++ + +P+D+N + E++K F RLN+DPDTI RV+DVNDR LR
Sbjct: 508 RVIPRGLIGRWNRICASHNMDPEDVNNASPELLKEFVRLNVDPDTIECNRVLDVNDRFLR 567
Query: 571 QITIGQAPTEKGFTRQTGFDITVASELMAILALSKDLKDLRERVGRIVIGSNYEKEPITV 630
I +G+A TEKG R+T FDI+VASE M+ILALS DL D+ R+ R+VI ++ IT
Sbjct: 568 SIEVGKASTEKGHVRKTSFDISVASECMSILALSCDLNDMHSRLSRMVIANDKYGNAITA 627
Query: 631 NDIGCAGAIAALLKDAVKPNLMQTLEGTPVLVHAGPFANISIGASSVIADRVALKLVGKP 690
D+G +GA+ LLKDA+KPNLMQTLEGTP VHAGPFANISIGASS+IAD++ALKL G
Sbjct: 628 GDLGVSGALTVLLKDAIKPNLMQTLEGTPAFVHAGPFANISIGASSIIADKIALKLAG-T 686
Query: 691 KEADESAESGYVVTEAGFDFTMGGERFFNIKCRASGLTPNAVVLVATVRALKLHGGAPDV 750
+ D ++GYVVTEAGF MG E+FFNIKCR S L PN VVLV TV+ALKLHGG P +
Sbjct: 687 ESFDRPEDAGYVVTEAGFASDMGMEKFFNIKCRYSKLVPNTVVLVTTVKALKLHGGGPKL 746
Query: 751 KPGQSLPEEYLKENVDLVRNGTANMCKQIENIKQFGVPVVVAINKFESDTEAEIDAIKEA 810
KPG +PEEYL EN+DLV+NG +NM K I+N +F +PVVVAIN +++D+ E + I+EA
Sbjct: 747 KPGAPIPEEYLVENLDLVKNGCSNMVKHIQNCHKFNIPVVVAINSYKTDSSKEHEIIREA 806
Query: 811 AQRVGAFDVVSTNHWAEGGKGAIKLAEAVIEASKQP--SEFKFLYDVNDTVENKLSTIVQ 868
A + GA D V ++HWA+GGKGAI+LA++V+ A Q S+F+ LYD ++E+K++ I +
Sbjct: 807 ALQAGAVDAVPSDHWAQGGKGAIELAKSVMTACDQSSNSKFRLLYDSETSIEDKVNVIAK 866
Query: 869 KMYGGANIEISEEAQKKIDMYRQQGFGDLPICIAKTQYSLSHDAKLKNVPTGFTFPIRDV 928
+MYG +E S A+++I+ + +QGFG+LPIC+AKTQYSLSH+ + +NVP FT PIRD+
Sbjct: 867 EMYGANGVEFSSLAKERINTFIKQGFGNLPICMAKTQYSLSHNPEFRNVPKNFTVPIRDM 926
Query: 929 RASIGAGYLYALAAEIQTIPGLSTHAGYMNVEIDEDGEIEGL 970
R + GAG++Y LAAEIQTIPGL T Y+N++I E+GEI GL
Sbjct: 927 RLNAGAGFIYPLAAEIQTIPGLPTAPAYLNIDICENGEIVGL 968
>Hs22055576 [H] KOG4230 C1-tetrahydrofolate synthase
Length = 755
Score = 536 bits (1382), Expect = e-152
Identities = 348/855 (40%), Positives = 456/855 (52%), Gaps = 152/855 (17%)
Query: 87 LKASEESGVKCIVEKLPEDIDELALLQKIQQVNDDDSIHGLLIQLPLPRHLNEAEVTNAV 146
LKA+EE G+K KLP E +++ I +N+D ++H R E V NA+
Sbjct: 33 LKAAEEIGMKATDIKLPRTTTESEVIKYIISLNEDSTVH---------RQYTEG-VINAI 82
Query: 147 SYQKDVDGFHRFNVGELSKRGGKPYFIPCTPNGCIRLLKESGIDLRGKRAVVIGRSDIVG 206
+ +KDVDG + G+L++ FIPC P GC+ L+KE+G+ + + AVV+G S IV
Sbjct: 83 APEKDVDGLTSISAGKLARGDLNDCFIPCMPKGCLELMKETGVQIARRHAVVVGCSKIVR 142
Query: 207 TPVASLLKDLNATVTICHRYTENIADVVKNADVVIAACGVPNMVKPNWLKQDAIVIDVGI 266
+ LL NATVT CH T N+ + +++ AIVI+ GI
Sbjct: 143 ALMRDLLLWNNATVTTCHSKTANLDEEGNGSNL-------------------AIVINCGI 183
Query: 267 NYVPDASKKSGRKLVGDVDFELCKDKASYITPVPGGVGPMTVAMLVQNVLLAAKRQREE- 325
NYVPD K K S++ MTVAML+Q+ + +AKR E+
Sbjct: 184 NYVPDDKKNKWEK--------------SWL---------MTVAMLMQSTVESAKRFLEKF 220
Query: 326 STRMPHIKPLPLKIEAKVPSDIEISRKQKPKYIEAVANELGVHPSELELYGHYKAKVSPK 385
I+ L ++ VPSDI+ISR KPK P E+ELYG KAKV
Sbjct: 221 KPGKWMIQYNNLNLKTPVPSDIDISRSCKPK------------PIEVELYGETKAKVLLS 268
Query: 386 IANRLADNENGKYVLVAGITPTPLGEGKSTTTIGLVQALSAHLHKPSIANVRQPSMGPTF 445
RL GKY +V GITPTPLGEGKSTTTIGLVQAL AHL++ VRQPS GPTF
Sbjct: 269 ALERLKHQPVGKYQVVTGITPTPLGEGKSTTTIGLVQALLAHLYQNFFMCVRQPSQGPTF 328
Query: 446 GVKXXXXXXXYAQVIPMDEFNMHLTGDIHXXXXXXXXXXXXXDTRMFHEATQKKHETFYK 505
G+K Y+QVIPM+EFN+HLTGDIH D ++FHE TQ TF
Sbjct: 329 GIKGGVAGGGYSQVIPMEEFNLHLTGDIHAITVSNNLVAAAIDAQIFHELTQTDKATF-T 387
Query: 506 RLVPVKKGVRSFTPSMLTRLNKLGINKTNPDDLNTEEIIKFARLNIDPDTITIKRVVDVN 565
LVP GVR F+ + RLN+LGI+KT+ L EEI +FARL+IDP+TI +RV++ N
Sbjct: 388 HLVPSVNGVRKFSDIHIQRLNRLGIDKTDSTTLTDEEINRFARLDIDPETIAWQRVLNTN 447
Query: 566 DRMLRQITIGQAPTEKGFTRQTGFDITVASELMAILALSKDLKDLRERVGRIVIGSNYEK 625
DR LR+ITIGQAPT+K T+ F
Sbjct: 448 DRFLRKITIGQAPTKKSHTQTVHF------------------------------------ 471
Query: 626 EPITVNDIGCAGAIAALLKDAVKPNLMQTLEGTPVLVHAGPFANISIGASSVIADRVALK 685
DI A I A+L A+ +L E +V A + A L
Sbjct: 472 ------DISVASEIMAVL--ALTTSLEDRRERLGKMVVASSKKGDPVSAED-------LG 516
Query: 686 LVGKPKEADESAESGYVVTEAGFDFTMGGERFFNIKCRASGLTPNAVVLVATVRALKLHG 745
LVG G+VVTEAGF +G E+F NIK + SGL P+ VVLVATV ALK+H
Sbjct: 517 LVGP---------EGFVVTEAGFGADIGIEKFLNIKSQYSGLHPHVVVLVATVSALKMHR 567
Query: 746 GAPDVKPGQSLPEEYLKENVDLVRNGTANMCKQIENIKQFGVPVVVAINKFESDTEAEID 805
G P V G LP+ Y++EN++LV G +N+ KQ EN + FG+PVVVA+N F++DTE E+D
Sbjct: 568 GGPMVTAGMPLPKAYIEENLELVEKGFSNLKKQTENARMFGIPVVVAVNAFKTDTETELD 627
Query: 806 AIKEAAQRVGAFDVVSTNHWAEGGKGAIKLAEAVIEASKQPSEFKFLYDVNDTVENKLST 865
I ++ AFD V HW +G + VE+K+
Sbjct: 628 LIGCLSREQRAFDAVKCTHWQKGAR--------------------------LPVEDKIRI 661
Query: 866 IVQKMYGGANIEISEEAQKKIDMYRQQGFGDLPICIAKTQYSLSHDAKLKNVPTGFTFPI 925
I QK+YG NIE+ +AQ K ++Y +QGFG+LPIC+AKT SLSH+ + K VPTGF PI
Sbjct: 662 IAQKIYGADNIELLLKAQHKAEVYTKQGFGNLPICMAKTHLSLSHNPERKGVPTGFILPI 721
Query: 926 RDVRASIGAGYLYAL 940
R++ ASIGAG+LY L
Sbjct: 722 RNIHASIGAGFLYPL 736
>At3g12290 [H] KOG0089 Methylenetetrahydrofolate
dehydrogenase/methylenetetrahydrofolate cyclohydrolase
Length = 299
Score = 292 bits (748), Expect = 2e-78
Identities = 142/286 (49%), Positives = 197/286 (68%), Gaps = 1/286 (0%)
Query: 36 LLSGTKIAKSIREEAFKEIKQIQIKSPSFEPCLKIIQVGTRPDSTAYVRMKLKASEESGV 95
++ G IA +IR E +E++ + K P L ++ VG+R DS YV K KA E G+
Sbjct: 11 IIDGKAIAHTIRSEIAEEVRGLSEKHGKV-PGLAVVIVGSRKDSQTYVNTKRKACAEVGI 69
Query: 96 KCIVEKLPEDIDELALLQKIQQVNDDDSIHGLLIQLPLPRHLNEAEVTNAVSYQKDVDGF 155
K LPE++ E L+ K+ ++N + +HG+L+QLPLP+H+NE + A+S KDVDGF
Sbjct: 70 KSFDVGLPEEVSEADLISKVHELNSNPDVHGILVQLPLPKHINEEHILGAISIDKDVDGF 129
Query: 156 HRFNVGELSKRGGKPYFIPCTPNGCIRLLKESGIDLRGKRAVVIGRSDIVGTPVASLLKD 215
H N+G+L+ +G +P F+PCTP GC+ LL SG+ ++G+RAVV+GRS+IVG PV+ LL
Sbjct: 130 HPLNIGKLAMKGREPLFLPCTPKGCLELLARSGVKIKGQRAVVVGRSNIVGLPVSLLLLK 189
Query: 216 LNATVTICHRYTENIADVVKNADVVIAACGVPNMVKPNWLKQDAIVIDVGINYVPDASKK 275
+ATVT H +T++ +++ AD+VIAACG +M+K NW+K A VIDVG N V D SKK
Sbjct: 190 ADATVTTVHSHTKDPEAIIREADIVIAACGQAHMIKGNWIKPGAAVIDVGTNAVSDPSKK 249
Query: 276 SGRKLVGDVDFELCKDKASYITPVPGGVGPMTVAMLVQNVLLAAKR 321
SG +LVGDVDF A +ITPVPGGVGPMTVAML++N + AKR
Sbjct: 250 SGYRLVGDVDFAEASKVAGFITPVPGGVGPMTVAMLLRNTVDGAKR 295
>At4g00620 [H] KOG0089 Methylenetetrahydrofolate
dehydrogenase/methylenetetrahydrofolate cyclohydrolase
Length = 360
Score = 287 bits (734), Expect = 6e-77
Identities = 144/286 (50%), Positives = 196/286 (68%), Gaps = 1/286 (0%)
Query: 36 LLSGTKIAKSIREEAFKEIKQIQIKSPSFEPCLKIIQVGTRPDSTAYVRMKLKASEESGV 95
++ G +AK IR+E E+ +++ +S P L +I VG R DS YVR K KA + G+
Sbjct: 71 VIDGKAVAKKIRDEITIEVSRMK-ESIGVIPGLAVILVGDRKDSATYVRNKKKACDSVGI 129
Query: 96 KCIVEKLPEDIDELALLQKIQQVNDDDSIHGLLIQLPLPRHLNEAEVTNAVSYQKDVDGF 155
K +L ED E +L+ + NDD S+HG+L+QLPLP H++E + NAVS +KDVDGF
Sbjct: 130 KSFEVRLAEDSSEEEVLKSVSGFNDDPSVHGILVQLPLPSHMDEQNILNAVSIEKDVDGF 189
Query: 156 HRFNVGELSKRGGKPYFIPCTPNGCIRLLKESGIDLRGKRAVVIGRSDIVGTPVASLLKD 215
H N+G L+ RG +P F+PCTP GCI LL I+++GKRAVVIGRS+IVG P A LL+
Sbjct: 190 HPLNIGRLAMRGREPLFVPCTPKGCIELLHRYNIEIKGKRAVVIGRSNIVGMPAALLLQR 249
Query: 216 LNATVTICHRYTENIADVVKNADVVIAACGVPNMVKPNWLKQDAIVIDVGINYVPDASKK 275
+ATV+I H T+N ++ + AD++I+A G PNMV+ +W+K A++IDVGIN V D S
Sbjct: 250 EDATVSIIHSRTKNPEEITREADIIISAVGQPNMVRGSWIKPGAVLIDVGINPVEDPSAA 309
Query: 276 SGRKLVGDVDFELCKDKASYITPVPGGVGPMTVAMLVQNVLLAAKR 321
G +LVGD+ +E AS ITPVPGGVGPMT+AML+ N L +AKR
Sbjct: 310 RGYRLVGDICYEEASKVASAITPVPGGVGPMTIAMLLSNTLTSAKR 355
>At2g38660 [H] KOG0089 Methylenetetrahydrofolate
dehydrogenase/methylenetetrahydrofolate cyclohydrolase
Length = 350
Score = 282 bits (722), Expect = 2e-75
Identities = 140/286 (48%), Positives = 202/286 (69%), Gaps = 1/286 (0%)
Query: 36 LLSGTKIAKSIREEAFKEIKQIQIKSPSFEPCLKIIQVGTRPDSTAYVRMKLKASEESGV 95
++ G IA+ IR + E+ +++ K+ P L ++ VG + DS YVR K+KA EE+G+
Sbjct: 63 VIDGNVIAEEIRTKIISEVGKMK-KAVGKVPGLAVVLVGEQRDSQTYVRNKIKACEETGI 121
Query: 96 KCIVEKLPEDIDELALLQKIQQVNDDDSIHGLLIQLPLPRHLNEAEVTNAVSYQKDVDGF 155
K ++ +LPED E ++ +++ N+D SIHG+L+QLPLP+HLNE+++ N V +KDVDGF
Sbjct: 122 KSVLAELPEDCTEGQIISVLRKFNEDTSIHGILVQLPLPQHLNESKILNMVRLEKDVDGF 181
Query: 156 HRFNVGELSKRGGKPYFIPCTPNGCIRLLKESGIDLRGKRAVVIGRSDIVGTPVASLLKD 215
H NVG L+ RG +P F+ CTP GC+ LL +G+++ GK AVVIGRS+IVG P++ LL+
Sbjct: 182 HPLNVGNLAMRGREPLFVSCTPKGCVELLIRTGVEIAGKNAVVIGRSNIVGLPMSLLLQR 241
Query: 216 LNATVTICHRYTENIADVVKNADVVIAACGVPNMVKPNWLKQDAIVIDVGINYVPDASKK 275
+ATV+ H +T++ + + AD+VIAA G+PN+V+ +WLK A+VIDVG V D+S +
Sbjct: 242 HDATVSTVHAFTKDPEHITRKADIVIAAAGIPNLVRGSWLKPGAVVIDVGTTPVEDSSCE 301
Query: 276 SGRKLVGDVDFELCKDKASYITPVPGGVGPMTVAMLVQNVLLAAKR 321
G +LVGDV +E AS ITPVPGGVGPMT+ ML+ N L AAKR
Sbjct: 302 FGYRLVGDVCYEEALGVASAITPVPGGVGPMTITMLLCNTLEAAKR 347
>At4g00600 [H] KOG0089 Methylenetetrahydrofolate
dehydrogenase/methylenetetrahydrofolate cyclohydrolase
Length = 310
Score = 237 bits (605), Expect = 6e-62
Identities = 116/218 (53%), Positives = 153/218 (69%)
Query: 104 EDIDELALLQKIQQVNDDDSIHGLLIQLPLPRHLNEAEVTNAVSYQKDVDGFHRFNVGEL 163
ED E +L+ + NDD S+HG+L+QLPLP H++E + NAVS +KDVDGFH N+G L
Sbjct: 88 EDSSEEEVLKYVSGFNDDPSVHGVLVQLPLPSHMDEQNILNAVSIEKDVDGFHPLNIGRL 147
Query: 164 SKRGGKPYFIPCTPNGCIRLLKESGIDLRGKRAVVIGRSDIVGTPVASLLKDLNATVTIC 223
+ RG +P F+PCTP GCI LL I+ +GKRAVVIGRS+IVG P A LL+ +ATV+I
Sbjct: 148 AMRGREPLFVPCTPKGCIELLHRYNIEFKGKRAVVIGRSNIVGMPAALLLQKEDATVSII 207
Query: 224 HRYTENIADVVKNADVVIAACGVPNMVKPNWLKQDAIVIDVGINYVPDASKKSGRKLVGD 283
H T N ++ + AD++I+A G PNMV+ +W+K A++IDVGI V D S G +LVGD
Sbjct: 208 HSRTMNPEELTRQADILISAVGKPNMVRGSWIKPGAVLIDVGIKPVEDPSAAGGERLVGD 267
Query: 284 VDFELCKDKASYITPVPGGVGPMTVAMLVQNVLLAAKR 321
+ + AS ITPVPG VGPMT+AML+ N L +AKR
Sbjct: 268 ICYVEASKIASAITPVPGDVGPMTIAMLLSNTLTSAKR 305
>Hs5729935 [H] KOG0089 Methylenetetrahydrofolate
dehydrogenase/methylenetetrahydrofolate cyclohydrolase
Length = 344
Score = 229 bits (583), Expect = 2e-59
Identities = 134/296 (45%), Positives = 184/296 (61%), Gaps = 14/296 (4%)
Query: 36 LLSGTKIAKSIREEAFKEIKQIQIKSPSFEPCLKIIQVGTRPDSTAYVRMKLKASEESGV 95
++SG K+A+ I++E +E+++ + S + P L +I VG P S +YV K +A+ G+
Sbjct: 33 VISGRKLAQQIKQEVRQEVEE-WVASGNKRPHLSVILVGENPASHSYVLNKTRAAAVVGI 91
Query: 96 KCIVEKLPEDIDELALLQKIQQVNDDDSIHGLLIQLPLPRHLNEAEVTNAVSYQKDVDGF 155
P I E LL I ++N+DD++ GLL+QLPLP H++E + NAVS KDVDGF
Sbjct: 92 NSETIMKPASISEEELLNLINKLNNDDNVDGLLVQLPLPEHIDERRICNAVSPDKDVDGF 151
Query: 156 HRFNVGELSKRGGKPYFIPCTPNGCIRLLKESGIDLRGKRAVVIGRSDIVGTPVASLL-- 213
H NVG + + +P TP G ++K +GI GK VV GRS VG P+A LL
Sbjct: 152 HVINVGRMCL--DQYSMLPATPWGVWEIIKRTGIPTLGKNVVVAGRSKNVGMPIAMLLHT 209
Query: 214 ------KDLNATVTICHRYT--ENIADVVKNADVVIAACGVPNMVKPNWLKQDAIVIDVG 265
+ATVTI HRYT E + AD+VI+A G+PN++ + +K+ A VIDVG
Sbjct: 210 DGAHERPGGDATVTISHRYTPKEQLKKHTILADIVISAAGIPNLITADMIKEGAAVIDVG 269
Query: 266 INYVPDASKKSGRKLVGDVDFELCKDKASYITPVPGGVGPMTVAMLVQNVLLAAKR 321
IN V D + KLVGDVDFE + KA YITPVPGGVGPMTVAML++N ++AAK+
Sbjct: 270 INRVHD-PVTAKPKLVGDVDFEGVRQKAGYITPVPGGVGPMTVAMLMKNTIIAAKK 324
>CE25047 [H] KOG4230 C1-tetrahydrofolate synthase
Length = 303
Score = 216 bits (549), Expect = 2e-55
Identities = 122/293 (41%), Positives = 179/293 (60%), Gaps = 8/293 (2%)
Query: 36 LLSGTKIAKSIREEAFKEIKQIQIKSPSFEPCLKIIQVGTRPDSTAYVRMKLKASEESGV 95
++SG + +K + + ++I + + P+F L I+QVG R DS Y+ KLK ++E G
Sbjct: 5 IVSGLEYSKKVLHDVGQKIAKTREHHPNFHAVLAIVQVGNRSDSNVYINSKLKKAKEIGA 64
Query: 96 KCIVEKLPEDIDELALLQKIQQVNDDDSIHGLLIQLPLP-RHLNEAE-VTNAVSYQKDVD 153
+ KLP+ I + L ++I +N D+ I G++IQLPL +H +A+ V + + KDVD
Sbjct: 65 DGKLIKLPDTITQGDLKREIMALNHDNEIDGIIIQLPLDCKHEIDADSVIDLIDPLKDVD 124
Query: 154 GFHRFNVGELSKRGGKPYFIPCTPNGCIRLLKESGID---LRGKRAVVIGRSDIVGTPVA 210
G R N G L++ + PCTP GC+ L++++ D + GK VV+GRS IVG+P A
Sbjct: 125 GLTRINAGRLARGELQRTIFPCTPFGCLYLVQQATGDANFVSGKEVVVLGRSKIVGSPAA 184
Query: 211 SLLKDLNATVTICHRYTENIADVVKNADVVIAACGVPNMVKPNWLKQDAIVIDVGINYVP 270
+L + TVTICH T N+ + AD++I A G + VK +W+K A VID GIN
Sbjct: 185 ALFLWHHGTVTICHSKTPNLKEKCLRADILIVAIGRKHFVKADWIKPGAFVIDCGINVGD 244
Query: 271 DASKKSGRKLVGDVDFELCKDKASYITPVPGGVGPMTVAMLVQNVLLAAKRQR 323
D + RK+ GDVD E K+ A ++TPVPGGVGPMTVAML++N AKR+R
Sbjct: 245 D---PNSRKIYGDVDTEAAKEVAGFLTPVPGGVGPMTVAMLIRNTFEQAKRRR 294
>7299133 [H] KOG0089 Methylenetetrahydrofolate
dehydrogenase/methylenetetrahydrofolate cyclohydrolase
Length = 303
Score = 214 bits (546), Expect = 4e-55
Identities = 127/301 (42%), Positives = 179/301 (59%), Gaps = 16/301 (5%)
Query: 36 LLSGTKIAKSIREEAFKEIKQIQIKSPSFEPCLKIIQVGTRPDSTAYVRMKLKASEESGV 95
++ G IA+ +R + E+K ++ +P L + VG P S YV K+ A E G+
Sbjct: 4 IIDGKAIAQEVRTQLAHELKGMEAAGYP-KPHLTAVIVGEDPASEKYVANKMVACREVGI 62
Query: 96 KCIVEKLPEDIDELALLQKIQQVNDDDSIHGLLIQLPLPRHLNEAEVTNAVSYQKDVDGF 155
++LP + LLQ I +N D + G+L+QLP+P H+NE + NAV KDVDGF
Sbjct: 63 SSETKRLPASTTQEELLQLIADLNKDPQVTGILVQLPVPEHINERTICNAVDVDKDVDGF 122
Query: 156 HRFNVGELSKRGGKPYFIPCTPNGCIRLLKESGIDLRGKRAVVIGRSDIVGTPVASLL-- 213
+ N+G + IP TP G RLL+ I+ G+ AVV+GRS V P+A LL
Sbjct: 123 NEVNIGRTAL--DMEANIPATPLGVKRLLEHMKIETLGRNAVVVGRSKNVSLPMAILLHA 180
Query: 214 ------KDLNATVTICHRYT--ENIADVVKNADVVIAACGVPNMVKPNWLKQDAIVIDVG 265
K ++ATVTICHRYT + +A + AD+++ A G P ++ + +K A VIDVG
Sbjct: 181 DGKYATKAMDATVTICHRYTPPKELARHCRQADIIVVAVGKPGLITKDMVKPGACVIDVG 240
Query: 266 INYVPDASKKSGR-KLVGDVDFELCKDKASYITPVPGGVGPMTVAMLVQNVLLAAKRQRE 324
IN + D S +G+ KLVGDVDFE + A +ITPVPGGVGPMTVAML+ N L AA++Q +
Sbjct: 241 INRIKDES--TGQFKLVGDVDFEEVRQVAGHITPVPGGVGPMTVAMLMHNTLKAARKQFD 298
Query: 325 E 325
+
Sbjct: 299 D 299
>Hs22053503 [H] KOG4230 C1-tetrahydrofolate synthase
Length = 218
Score = 207 bits (528), Expect = 5e-53
Identities = 111/203 (54%), Positives = 133/203 (64%), Gaps = 1/203 (0%)
Query: 323 REESTRMPHIKPLPLKIEAKVPSDIEISRKQKPKYIEAVANELGVHPSELELYGHYKAKV 382
RE+ R + L L+ + VPSDIEISR Q PK ++ +A E+G+ E+E+YG KAKV
Sbjct: 10 REQQHRRWRLHCLKLQPLSPVPSDIEISRGQTPKAVDVLAKEIGLLADEIEIYGKSKAKV 69
Query: 383 SPKIANRLADNENGKYVLVAGITPTPLGEGKSTTTIGLVQALSAHLHKPSIANVRQPSMG 442
+ RL D +GKYVLVAGITPTPLGEGKST TIGLVQAL+AHL+ S A +RQPS G
Sbjct: 70 RLSVLERLKDQADGKYVLVAGITPTPLGEGKSTVTIGLVQALTAHLNVNSFACLRQPSQG 129
Query: 443 PTFGVKXXXXXXXYAQVIPMDEFNMHLTGDIHXXXXXXXXXXXXXDTRMFHEATQKKHET 502
PTFGVK YAQVIPM+EFN+HLTGDIH DTR+ HE TQ +
Sbjct: 130 PTFGVKGGAAGGGYAQVIPMEEFNLHLTGDIHAITAANNLLAAAIDTRILHENTQ-TDKA 188
Query: 503 FYKRLVPVKKGVRSFTPSMLTRL 525
Y RLVP+ GVR F+ L RL
Sbjct: 189 LYNRLVPLVNGVREFSEIQLARL 211
>Hs13376513_2 [H] KOG0089 Methylenetetrahydrofolate
dehydrogenase/methylenetetrahydrofolate cyclohydrolase
Length = 196
Score = 163 bits (413), Expect = 1e-39
Identities = 94/196 (47%), Positives = 122/196 (61%), Gaps = 13/196 (6%)
Query: 136 HLNEAEVTNAVSYQKDVDGFHRFNVGELSKRGGKPYFIPCTPNGCIRLLKESGIDLRGKR 195
H++E + N ++ +KDVDGFH N+G L + IP T + ++K +GI GK
Sbjct: 1 HVDERTICNGIAPEKDVDGFHIINIGRLCL--DQHSLIPATASAVWEIIKRTGIQTFGKN 58
Query: 196 AVVIGRSDIVGTPVASLLKDL--------NATVTICHRYT--ENIADVVKNADVVIAACG 245
VV GRS VG P+A LL +ATVTI HRYT E + + AD++I A G
Sbjct: 59 VVVAGRSKNVGMPIAMLLHTDGEHERPGGDATVTIAHRYTPKEQLKIHTQLADIIIVAAG 118
Query: 246 VPNMVKPNWLKQDAIVIDVGINYVPDASKKSGRKLVGDVDFELCKDKASYITPVPGGVGP 305
+P ++ + +K+ A VIDVGINYV D KLVGDVDFE K KA +ITPVPGGVGP
Sbjct: 119 IPKLITSDMVKEGAAVIDVGINYVHDPVTGK-TKLVGDVDFEAVKKKAGFITPVPGGVGP 177
Query: 306 MTVAMLVQNVLLAAKR 321
MTVAML++N LLAAK+
Sbjct: 178 MTVAMLLKNTLLAAKK 193
>Hs22040978 [H] KOG0089 Methylenetetrahydrofolate
dehydrogenase/methylenetetrahydrofolate cyclohydrolase
Length = 402
Score = 139 bits (349), Expect = 3e-32
Identities = 97/277 (35%), Positives = 139/277 (50%), Gaps = 48/277 (17%)
Query: 36 LLSGTKIAKSIREEAFKEIKQIQIKSPSFEPCLKIIQVGTRPDSTAYVRMKLKASEESGV 95
++SG K+A+ I++E +E+++ + S P L +I VG S +YV K +A+ + G+
Sbjct: 154 VISGRKLAQQIKQEVRQEVEE-WVASGDKRPHLSMILVGESSASHSYVLNKTRAAADVGI 212
Query: 96 KCIVEKLPEDIDELALLQKIQQVNDDDSIHGLLIQLPLPRHLNEAEVTNAVSYQKDVDGF 155
RH++E + NAVS KD DGF
Sbjct: 213 NTASS----------------------------------RHIDERRICNAVSPDKDADGF 238
Query: 156 HRFNVGELSKRGGKPYFIPCTPNGCIRLLKESGIDLRGKRAVVIGRSDIVGTPVASLLKD 215
H N G + + +P TP G ++K + I GK VV GRS V P+A LL
Sbjct: 239 HVINAGRMCL--DQYSMLPATPCGVWEIIKRTDIPTLGKNVVVAGRSKSVEMPIAMLLHT 296
Query: 216 L--------NATVTICHRYT--ENIADVVKNADVVIAACGVPNMVKPNWLKQDAIVIDVG 265
+ATVTI +RYT E + AD+VI+A G+PN++ + +K++A V D+G
Sbjct: 297 DGVHERPGGDATVTISYRYTSKEQLKKHTILADIVISAAGIPNLITADMIKEEAAVTDMG 356
Query: 266 INYVPDASKKSGRKLVGDVDFELCKDKASYITPVPGG 302
IN V D + KLVGDVDFE + KA YITPVPGG
Sbjct: 357 INRVHD-PVTAKLKLVGDVDFEGVRQKAGYITPVPGG 392
>Hs20541728 [H] KOG0089 Methylenetetrahydrofolate
dehydrogenase/methylenetetrahydrofolate cyclohydrolase
Length = 277
Score = 89.4 bits (220), Expect = 3e-17
Identities = 45/121 (37%), Positives = 74/121 (60%), Gaps = 1/121 (0%)
Query: 41 KIAKSIREEAFKEIKQIQIKSPSFEPCLKIIQVGTRPDSTAYVRMKLKASEESGVKCIVE 100
K+A+ I++E +E+++ Q+ S + PCL + V P S ++ K +A+ + G+ +
Sbjct: 83 KLAQWIKQEVRQEVEE-QVASGNKGPCLSVALVSKTPASLCHILNKTRAAADVGINSEII 141
Query: 101 KLPEDIDELALLQKIQQVNDDDSIHGLLIQLPLPRHLNEAEVTNAVSYQKDVDGFHRFNV 160
P I E LL I ++N+D ++ GLL+ LPLP H++E ++ N+V KDVDGFH NV
Sbjct: 142 VKPASISEEELLNLINKLNNDGNVDGLLLHLPLPEHIDERKICNSVFPDKDVDGFHVINV 201
Query: 161 G 161
G
Sbjct: 202 G 202
>YKR080w [H] KOG0089 Methylenetetrahydrofolate
dehydrogenase/methylenetetrahydrofolate cyclohydrolase
Length = 320
Score = 84.0 bits (206), Expect = 1e-15
Identities = 80/306 (26%), Positives = 138/306 (44%), Gaps = 37/306 (12%)
Query: 40 TKIAKSIREEAFKEIKQIQIKSPSFEPCLKIIQVGTRPDSTAYVRMKLKASEESGVKCIV 99
+K+A++ E +++ + P L P + Y K SE G + +
Sbjct: 11 SKVAETFNTEIINNVEEYKKTHNGQGPLLVGFLANNDPAAKMYATWTQKTSESMGFRYDL 70
Query: 100 EKLPEDIDELALLQKIQQVNDDDSIHGLLIQLPLPRHLNEAEVTNAVSYQKDVDGFHRF- 158
+ ED D L + I Q N DDS++G+++ P+ + + + V +KDV+G +
Sbjct: 71 RVI-EDKDFLE--EAIIQANGDDSVNGIMVYFPVFGNAQDQYLQQVVCKEKDVEGLNHVY 127
Query: 159 ------NVGELSKRGGKPYFIPCTPNGCIRLLK---------ESGIDLRGKRAVVIGRSD 203
NV L K +PCTP +++L+ G L GK+ +VI RS+
Sbjct: 128 YQNLYHNVRYLDKENRLKSILPCTPLAIVKILEFLKIYNNLLPEGNRLYGKKCIVINRSE 187
Query: 204 IVGTPVASLLKDLNATVTICHRYTENIADVVKNADVVIAACGVPNM--VKPNWLKQDAIV 261
IVG P+A+LL + ATV NI + + + V ++ + LK+ ++
Sbjct: 188 IVGRPLAALLANDGATVYSVD--VNNIQKFTRGESLKLNKHHVEDLGEYSEDLLKKCSLD 245
Query: 262 IDVGINYVPDASKKSGRKLVGD----VDF-------ELCKDKASYITPVPGGVGPMTVAM 310
DV I VP + K + + + ++F + K+KAS P+ G V T+AM
Sbjct: 246 SDVVITGVPSENYKFPTEYIKEGAVCINFACTKNFSDDVKEKASLYVPMTGKV---TIAM 302
Query: 311 LVQNVL 316
L++N+L
Sbjct: 303 LLRNML 308
>Hs20542451 [H] KOG0089 Methylenetetrahydrofolate
dehydrogenase/methylenetetrahydrofolate cyclohydrolase
Length = 306
Score = 82.0 bits (201), Expect = 4e-15
Identities = 55/141 (39%), Positives = 77/141 (54%), Gaps = 12/141 (8%)
Query: 141 EVTNAVSYQKDVDGFHRFNVGELSKRGGKPYFIPCTPNGCIRLLKESGIDLRGKRAVVIG 200
EV V+ KDVD F+ NVG++ + +P P G ++K++GI GK VV G
Sbjct: 161 EVEEWVASDKDVDDFYVINVGQMCL--DQYSTLPAAPWGVWEIIKQTGIPTLGKSVVVAG 218
Query: 201 RSDIVGTPVASLLKDL--------NATVTICHRYT--ENIADVVKNADVVIAACGVPNMV 250
RS V PVA LL +ATVTI H YT E + AD+VI+A +PN++
Sbjct: 219 RSKNVEMPVAMLLHTDGAHEGPRGDATVTISHGYTPKEQLKKHTILADIVISAADIPNLI 278
Query: 251 KPNWLKQDAIVIDVGINYVPD 271
+ +K+ A +I+VGIN V D
Sbjct: 279 TAHMIKEGAAIINVGINRVQD 299
>SPBC1711.04 [H] KOG0089 Methylenetetrahydrofolate
dehydrogenase/methylenetetrahydrofolate cyclohydrolase
Length = 320
Score = 75.9 bits (185), Expect = 3e-13
Identities = 79/307 (25%), Positives = 136/307 (43%), Gaps = 50/307 (16%)
Query: 43 AKSIREEAFKEIKQIQIKSPSFEPCLKIIQVGTRPDSTAYVRMKLKASEESGVKCIVEKL 102
A + E +++KQ + F P L + P + Y K E G + + ++
Sbjct: 18 ASRVAETFVEQLKQ-HVNLFEFAPKLVGFLSNSDPAARMYADWTNKTCTEIGFQYELREV 76
Query: 103 PEDIDELALLQKIQQVNDDDSIHGLLIQLPLPRHLNEAEVTNAVSYQKDVDGF-HRF--- 158
P+D E A+++ N+D S++G++I P+ + + VS KDV+G H++
Sbjct: 77 PKDDLEDAIVE----ANNDPSVNGIMIYFPVFNDGQDQYLQQVVSPDKDVEGLCHKYVMN 132
Query: 159 ---NVGELSKRGGKPYFIPCTPNGCIRLLKESGI---------DLRGKRAVVIGRSDIVG 206
N+ L K +PCTP +++L+ G+ L GK ++ RS+IVG
Sbjct: 133 MYHNIRHLDPEKTKKSILPCTPLAIVKILEYLGVYNKIINYGNRLYGKTITIVNRSEIVG 192
Query: 207 TPVASLLKDLNATVTICHRYTENIADVVKNADVVIAACGV----PNMVKPNWLKQD-AIV 261
P+A+LL + A V Y+ +I N G+ ++ N+ +D A +
Sbjct: 193 RPLAALLANDGAKV-----YSVDI----HNVQCFTRGAGIRSKKHDVADTNFKLEDVAPI 243
Query: 262 IDVGINYVPDASKKSGRKLVGDVDFELC------------KDKASYITPVPGGVGPMTVA 309
DV I VP A+ K LV D +C K+ A P +G +T+A
Sbjct: 244 SDVIICGVPSANYKFPSNLVRDGAVCICFSSEKNFDAASLKEHAGIYVP---SIGKVTIA 300
Query: 310 MLVQNVL 316
ML++N++
Sbjct: 301 MLLRNLI 307
>Hs17437830 [H] KOG0089 Methylenetetrahydrofolate
dehydrogenase/methylenetetrahydrofolate cyclohydrolase
Length = 106
Score = 55.1 bits (131), Expect = 5e-07
Identities = 32/93 (34%), Positives = 52/93 (55%), Gaps = 1/93 (1%)
Query: 42 IAKSIREEAFKEIKQIQIKSPSFEPCLKIIQVGTRPDSTAYVRMKLKASEESGVKCIVEK 101
+AK I++E + ++ + + P L II VG P S YVR K++A+ G+ +
Sbjct: 1 MAKHIQKEIQRGVES-WVSLGNRRPHLSIILVGDNPASHTYVRNKIRAASAVGICSELIL 59
Query: 102 LPEDIDELALLQKIQQVNDDDSIHGLLIQLPLP 134
P+D+ + LL Q+N D + G+L+QLPLP
Sbjct: 60 KPKDVSQEELLDVTDQLNMDPRVSGILVQLPLP 92
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.135 0.384
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 55,648,143
Number of Sequences: 60738
Number of extensions: 2415933
Number of successful extensions: 6242
Number of sequences better than 1.0e-05: 21
Number of HSP's better than 0.0 without gapping: 17
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 6165
Number of HSP's gapped (non-prelim): 27
length of query: 971
length of database: 30,389,216
effective HSP length: 116
effective length of query: 855
effective length of database: 23,343,608
effective search space: 19958784840
effective search space used: 19958784840
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)