ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV3260 check: MH R KOG0017 General function prediction only FOG: Transposon-encoded proteins with TYA, reverse transcriptase, integrase domains in various combinations
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV3260 1128947 1130284 446
(446 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDR316w [R] KOG0017 FOG: Transposon-encoded proteins with TYA re... 483 e-136
SPBC3B9.04 [R] KOG4300 Predicted methyltransferase 145 1e-34
SPBC1347.09 [H] KOG1270 Methyltransferases 51 4e-06
At1g69520 [R] KOG4300 Predicted methyltransferase 51 4e-06
>YDR316w [R] KOG0017 FOG: Transposon-encoded proteins with TYA reverse
transcriptase integrase domains in various combinations
Length = 471
Score = 483 bits (1244), Expect = e-136
Identities = 243/423 (57%), Positives = 306/423 (71%), Gaps = 13/423 (3%)
Query: 33 RCKSTYIPSPDSVYKKPKSKEQIAREKHEADLKSDNWIIRYGAIARSEKFSKALTKYILA 92
+ K+ + + + V +K K+KE++A+E+ E L+S N +R+GAIARSEKFSK +TKY++
Sbjct: 52 KLKNVFQMNSNRVIRKQKTKEELAKERFEEQLRSPNRFVRWGAIARSEKFSKGMTKYMIG 111
Query: 93 AYVVFILYGLNXXXXXXXXXXXXXXISAKIDMGVANEYELLRYKELKNTLRTRDVEKLKQ 152
AYV+F++YGL + K + G ANEYE LR KELK LR RD KL++
Sbjct: 112 AYVIFLIYGLFFTKKLFAKDKELERLLKKQEEGNANEYEALRIKELKGKLRRRDELKLEE 171
Query: 153 YQKIIEKDGTENLDKITLENNDQNKINEKILPARDTTDFYEMKAEEYDEGVSFEEMMIRM 212
Y+K+ +++G EN D I ++N DQNK+NE+ILPARDTT+FY+ KA EYD+ ++ EE +I +
Sbjct: 172 YKKM-QEEGIENFDDIRVQNFDQNKLNEQILPARDTTNFYQEKANEYDKAINMEERVIFL 230
Query: 213 GKRRKWLMKHCKGDVLEVACGTGRNIKYLDPTKINSITFLDASEKMMEITNKKFREALPN 272
GKRRKWLMKHC+GDVLEV+CGTGRNIKYLD ++INSITFLD+SE MMEIT+KKFRE P
Sbjct: 231 GKRRKWLMKHCQGDVLEVSCGTGRNIKYLDMSRINSITFLDSSENMMEITHKKFREKFPK 290
Query: 273 FKKCAFVVGKAEDLIDL---------NSKETPVKYDTIVEAFGLCSHHDPVKSLKNFAEL 323
+KK AFVVGKAE+L+DL N KE VKYDTIVEAFGLCSH DPVK+L NF +L
Sbjct: 291 YKKVAFVVGKAENLVDLAEKGKPSLENEKENQVKYDTIVEAFGLCSHEDPVKALNNFGKL 350
Query: 324 LKPGGRIILLEHGRGSYDTINKILDDRAEKRLKTWGCRWNLDIGEILDDSGLDVVEERRT 383
LKP GRIILLEHGRG YD INKILD+RAE+RL TWGCRWNLD+GE+LDDS L++VEE+RT
Sbjct: 351 LKPDGRIILLEHGRGQYDFINKILDNRAERRLNTWGCRWNLDLGEVLDDSDLELVEEKRT 410
Query: 384 HFGTTWCIVAXXXXXXXXXXXXXXXXXYLGASVKSRIESFTNDGKKQDSLQATHNKPTSE 443
H GTTWCIVA YL +S++ R+ESF KK D +P
Sbjct: 411 HLGTTWCIVAKRKGDVKKKDELGFVEKYLQSSIRKRMESFE---KKDDMASKKELEPVPP 467
Query: 444 TSK 446
SK
Sbjct: 468 VSK 470
>SPBC3B9.04 [R] KOG4300 Predicted methyltransferase
Length = 248
Score = 145 bits (366), Expect = 1e-34
Identities = 77/223 (34%), Positives = 127/223 (56%), Gaps = 9/223 (4%)
Query: 172 NNDQNKINEKILPARDTTDFYEMKAEEYDEGVSFEEMMIRMGKRRKWLMKHCKGDVLEVA 231
N + + ++ ++P ++ Y A++Y ++ EE+ + R +L+++ KGDVLEV
Sbjct: 33 NYESRQKSDLLIPNSNSISIYNQIADKYSRKITREEIFSGIYFLRYFLLRNAKGDVLEVG 92
Query: 232 CGTGRNIKYLDPTKINSITFLDASEKMMEITNKKFREALP-NFKKCAFVVGKAEDLIDLN 290
G G N + KIN++T ++ +EKM EI + + ++ +P N F DL
Sbjct: 93 SGPGTNFPFYKWKKINTLTLVEPAEKMREIADARAKKKVPPNVLYRQFA--------DLR 144
Query: 291 SKETPVKYDTIVEAFGLCSHHDPVKSLKNFAELLKPGGRIILLEHGRGSYDTINKILDDR 350
YDTI++ F +CS V+ L N+ LL+ GRI+L+EHG+G Y +N+IL+
Sbjct: 145 QLPPNQSYDTIIQTFCICSQEKAVEQLNNYRSLLRSDGRILLIEHGKGKYKFLNRILNAY 204
Query: 351 AEKRLKTWGCRWNLDIGEILDDSGLDVVEERRTHFGTTWCIVA 393
AE ++WGC WN DI ++L+DS L + +R +FGTT+ I A
Sbjct: 205 AESHYESWGCVWNRDIEQLLEDSELTIDSCKRFNFGTTYVIEA 247
>SPBC1347.09 [H] KOG1270 Methyltransferases
Length = 284
Score = 50.8 bits (120), Expect = 4e-06
Identities = 32/113 (28%), Positives = 60/113 (52%), Gaps = 3/113 (2%)
Query: 227 VLEVACGTGRNIKYLDPTKINSITFLDASEKMMEITNKKFREA-LPNFKKCAFVVGKAED 285
+L+ ACGTG ++L P I +D S+ M+++ N+KFR+ +P + CA+V+ +D
Sbjct: 81 ILDFACGTGLISQHLFPY-CKQIVGIDVSQDMVDVYNEKFRKMNIPKERACAYVLS-LDD 138
Query: 286 LIDLNSKETPVKYDTIVEAFGLCSHHDPVKSLKNFAELLKPGGRIILLEHGRG 338
L + ++D +V + D + ++LLKP GR+ + + +G
Sbjct: 139 LDGNGDEPFSTEFDAVVCSMAYHHIKDLQEVTNKLSKLLKPNGRLFVADLIKG 191
>At1g69520 [R] KOG4300 Predicted methyltransferase
Length = 795
Score = 50.8 bits (120), Expect = 4e-06
Identities = 36/111 (32%), Positives = 52/111 (46%), Gaps = 9/111 (8%)
Query: 227 VLEVACGTGRNIKYLDPTKINSITFLDASEKMMEITNKKFREALPNFKKCAFV--VGKAE 284
VLE+ GTG N KY S+ +D + KM K EA ++ FV +G++
Sbjct: 320 VLEIGIGTGPNFKYYTDIPNVSVIGIDPNAKMESYARKSATEAGLKPEEFTFVHALGESI 379
Query: 285 DLIDLNSKETPVKYDTIVEAFGLCSHHDPVKSLKNFAELLKPGGRIILLEH 335
L D + D +V LCS D ++LK +L+PGG I +EH
Sbjct: 380 PLEDAS-------VDAVVGTLVLCSVTDVTQTLKEIKRILRPGGIYIFIEH 423
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.136 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 25,036,170
Number of Sequences: 60738
Number of extensions: 1030021
Number of successful extensions: 3232
Number of sequences better than 1.0e-05: 4
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 3221
Number of HSP's gapped (non-prelim): 6
length of query: 446
length of database: 30,389,216
effective HSP length: 110
effective length of query: 336
effective length of database: 23,708,036
effective search space: 7965900096
effective search space used: 7965900096
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)