ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV3271 good A KOG2078 RNA processing and modification tRNA modification enzyme
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV3271 1131727 1133232 502
(502 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YHR070w [A] KOG2078 tRNA modification enzyme 487 e-137
Hs14752839 [A] KOG2078 tRNA modification enzyme 228 2e-59
7292320 [A] KOG2078 tRNA modification enzyme 204 3e-52
At3g56120 [A] KOG2078 tRNA modification enzyme 199 1e-50
CE17599_2 [A] KOG2078 tRNA modification enzyme 156 6e-38
ECU04g0620 [A] KOG2078 tRNA modification enzyme 140 3e-33
At4g27340 [A] KOG2078 tRNA modification enzyme 130 6e-30
>YHR070w [A] KOG2078 tRNA modification enzyme
Length = 499
Score = 487 bits (1254), Expect = e-137
Identities = 260/512 (50%), Positives = 342/512 (66%), Gaps = 26/512 (5%)
Query: 1 MRFGLVLRRTMSRAIET-----TFKYEPPVHRGMKQLDKSLFXXXXXXXXXXXXXXXXIS 55
M+ L + + +R I + F Y PPV+R M++LD+S F IS
Sbjct: 1 MKIALPVFQKFNRLISSCKMSGVFPYNPPVNRQMRELDRSFFITKIPMCAVKFPEPKNIS 60
Query: 56 VFTKKFKDDILRTPRIPFVIRLGETPKEDTDKNDVEPVQKKSKSLANDNH--ITKGIILK 113
VF+K FK+ ILR PRIP V++L + +D ++ VQ K A+ N+ +TKG++L
Sbjct: 61 VFSKNFKNCILRVPRIPHVVKLNSSKPKD----ELTSVQNKKLKTADGNNTPVTKGVLLH 116
Query: 114 DSIQDVSQARGKLTSNALEFLDEQEYELRNHEYVLDYDFWKVEEILNAILPEEYLAEIPT 173
+SI V A GKL +AL FL E E+ HEYVLDYDFWK EEIL A+LPE++L E+PT
Sbjct: 117 ESIHSVEDAYGKLPEDALAFLKENSAEIVPHEYVLDYDFWKAEEILRAVLPEQFLEEVPT 176
Query: 174 GFTIVGHVAHLNLRKEFKPFGEIIGQVILDKNSTIKTVVDKVDSIATKFRTFEMNVLAGE 233
GFTI GH+AHLNLR EFKPF +IGQVILDKN+ I+ VVDKV SIAT+FRTF M V+AG+
Sbjct: 177 GFTITGHIAHLNLRTEFKPFDSLIGQVILDKNNKIECVVDKVSSIATQFRTFPMKVIAGK 236
Query: 234 -PNLLVTQRESDCSFTFDFSKVYWNSRLHTEHARLV-SLFKPGQIVGDVFAGVGPFSVPA 291
+L+V Q+ES+C+F FDFSKVYWNSRLHTEH RLV F+PGQ+V DVFAGVGPF+VPA
Sbjct: 237 SDSLVVEQKESNCTFKFDFSKVYWNSRLHTEHERLVKQYFQPGQVVCDVFAGVGPFAVPA 296
Query: 292 GKKKVIVLSNDLNPESYKYMQQNIIDNKVGNFVEPLNLDGREFIRDSPKLLQQFIERVQG 351
GKK VIVL+NDLNPESYKY+++NI NKV V+ N+DG +FIR SP+LLQQ+I+ +G
Sbjct: 297 GKKDVIVLANDLNPESYKYLKENIALNKVAKTVKSFNMDGADFIRQSPQLLQQWIQDEEG 356
Query: 352 ---VITVPGGKKYKDKVTGETK-RTPETKIPIKNQFFDHYVMNLPDSALQFLDEFVGLYS 407
I +P K+++ + + + P TK I HYVMNLPDSA+ FL F G+++
Sbjct: 357 GKITIPLPLKKRHRSQQHNDQQPPQPRTKELIIPSHISHYVMNLPDSAISFLGNFRGIFA 416
Query: 408 RYGFTYEQMVQEHGEQFQTPWIHCHCFHKYDAEEQPEPSMEQLHERVYQQILQIMKTTEQ 467
+ + + Q PW+H HCF KY +Q + ++LH RV+ +I+ +K T
Sbjct: 417 AH-------TKGATDTIQMPWVHVHCFEKYPPGDQ--VTEDELHARVHARIIAALKVTAD 467
Query: 468 ELPFNQFQFHLVRKVAPTKPMFCVSFQLPQSL 499
+LP N HLVRKVAPTKPM+C SFQLP ++
Sbjct: 468 DLPLNAVSLHLVRKVAPTKPMYCASFQLPANV 499
>Hs14752839 [A] KOG2078 tRNA modification enzyme
Length = 469
Score = 228 bits (581), Expect = 2e-59
Identities = 160/486 (32%), Positives = 239/486 (48%), Gaps = 80/486 (16%)
Query: 16 ETTFKYEPPVHRGMKQLDKSLFXXXXXXXXXXXXXXXXISVFTKKFKDDILRTPRIPFVI 75
ET P RGM +LD++ F +S + K L+ P I VI
Sbjct: 29 ETELFSPPSDVRGMTKLDRTAFKKTVNIPVLKVRKEI-VSKLMRSLKRAALQRPGIRRVI 87
Query: 76 RLGETPKEDTDKNDVEPVQKKSKSLANDNHITKGIILKDSIQDVSQARGKLTSNALEFLD 135
+P K+S+ + D + I DS + K + LE L+
Sbjct: 88 E--------------DPEDKESRLIMLDPY---KIFTHDSFE-------KAELSVLEQLN 123
Query: 136 EQEYELRNHEYVLDYDFWKVEEILNAILPEEYLAEIPTGFTIVGHVAHLNLRKEFKPFGE 195
++ + L Y+ +K EEIL A+LPE ++ +GF+ +GH+AHLNLR F
Sbjct: 124 VSP-QISKYNLELTYEHFKSEEILRAVLPEGQ--DVTSGFSRIGHIAHLNLRDHQLSFKH 180
Query: 196 IIGQVILDKNSTIKTVVDKVDSIATKFRTFEMNVLAGEPNLLVTQRESDCSFTFDFSKVY 255
+IGQV++DKN I + V+K+++I +R F+M VL+GE N++ RE++ ++ FDFSKVY
Sbjct: 181 LIGQVMIDKNPGITSAVNKINNIDNMYRNFQMEVLSGEQNMMTKVRENNYTYEFDFSKVY 240
Query: 256 WNSRLHTEHARLVSLFKPGQIVGDVFAGVGPFSVPAGKKKVIVLSNDLNPESYKYMQQNI 315
WN RL TEH+R+ L KPG ++ DVFAGVGPF++P KK V +NDLNPES+K++ N
Sbjct: 241 WNPRLSTEHSRITELLKPGDVLFDVFAGVGPFAIPVAKKNCTVFANDLNPESHKWLLYNC 300
Query: 316 IDNKVGNFVEPLNLDGREFIRDSPKLLQQFIERVQGVITVPGGKKYKDKVTGETKRTPET 375
NKV V+ NLDG++F++ K + + ++ G+ K+ K V
Sbjct: 301 KLNKVDQKVKVFNLDGKDFLQGPVK---EELMQLLGL-----SKERKPSV---------- 342
Query: 376 KIPIKNQFFDHYVMNLPDSALQFLDEFVGLYSRYGFTYEQMVQEHGEQFQTPWIHCHCFH 435
H VMNLP A++FL F L + E + P +HC+ F
Sbjct: 343 ----------HVVMNLPAKAIEFLSAFKWLLDGQPCSSEFL----------PIVHCYSFS 382
Query: 436 KYDAEEQPEPSMEQLHERVYQQILQIMKTTEQELPFNQFQFHLVRKVAPTKPMFCVSFQL 495
K + P + Q V L+ + HLVR VAP K M C++FQ+
Sbjct: 383 K---DANPAEDVRQRAGAVLGISLEACSSV-----------HLVRNVAPNKEMLCITFQI 428
Query: 496 PQSLAF 501
P S+ +
Sbjct: 429 PASVLY 434
>7292320 [A] KOG2078 tRNA modification enzyme
Length = 537
Score = 204 bits (518), Expect = 3e-52
Identities = 132/367 (35%), Positives = 187/367 (49%), Gaps = 51/367 (13%)
Query: 132 EFLDEQEYELRNHEYV---LDYDFWKVEEILNAILPEEYLAEIPTGFTIVGHVAHLNLRK 188
E L Q+ N + L Y+ W EIL ++LP E E T ++ +GH+AHLNLR
Sbjct: 84 EDLQRQKVNAENFSFADLELRYENWSANEILKSVLPTE--EEGLTSYSRIGHIAHLNLRD 141
Query: 189 EFKPFGEIIGQVILDKNSTIKTVVDKVDSIATKFRTFEMNVLAGEPNLLVTQRESDCSFT 248
P+ ++IGQV+ DK +TVV+K SI +R F++ ++ G+P+ V +E+ F
Sbjct: 142 HLLPYKQLIGQVLRDKLPNCRTVVNKASSIDNTYRNFQLELICGDPDYQVETKENGVPFE 201
Query: 249 FDFSKVYWNSRLHTEHARLVSLFKPGQIVGDVFAGVGPFSVPAGKKKVIVLSNDLNPESY 308
FDFSKVYWN RL TEH R+V + K ++ DVFAGVGPFS+PA KK+ VL+NDLNPES+
Sbjct: 202 FDFSKVYWNPRLSTEHERIVKMLKSDDVLYDVFAGVGPFSIPAAKKRCHVLANDLNPESF 261
Query: 309 KYMQQNIIDNKVGNFVEPLNLDGREFIRDSPKLLQQFIERVQGVITVPGGKKYKDKVTGE 368
+++Q N NK ++ N DGR+FI + +L + ++R+ T G
Sbjct: 262 RWLQHNAKRNKCLPNIKMSNKDGRQFIVE--ELREDLLKRLCTTDTTTYG---------- 309
Query: 369 TKRTPETKIPIKNQFFDHYVMNLPDSALQFLDEFVGLYSRYGFTYEQMVQEHGEQFQTPW 428
H MNLP A++FLD F GLYS + + P
Sbjct: 310 ----------------IHITMNLPAMAVEFLDAFRGLYS------ADELAQLPTNVCYPT 347
Query: 429 IHCHCFHKYDAEEQPEPSMEQLHERVYQQILQIMKTTEQELPFNQFQFHLVRKVAPTKPM 488
+H + F K E E V Q + + + E F VR VAP K M
Sbjct: 348 VHVYSFAK----------GENTKELVRQLVESNLGASLDENLLQGINF--VRNVAPNKDM 395
Query: 489 FCVSFQL 495
+ VSF+L
Sbjct: 396 YRVSFKL 402
>At3g56120 [A] KOG2078 tRNA modification enzyme
Length = 447
Score = 199 bits (505), Expect = 1e-50
Identities = 140/399 (35%), Positives = 193/399 (48%), Gaps = 84/399 (21%)
Query: 156 EEILNAILPEEYLAEIPTGFTI------VGHVAHLNLRKEFKPFGEIIGQVILDKN-STI 208
EE LN + L +P T+ GH+AHLNL E PF ++I +VI DKN I
Sbjct: 71 EEKLNQLKKLSELEVVPHSVTLGYSYWSAGHIAHLNLHDELLPFKDVIAKVIYDKNYPRI 130
Query: 209 KTVVDKVDSIATKFRTFEMNVLAGEPNLLVTQRESDCSFTFDFSKVYWNSRLHTEHARLV 268
KT+V+KV +I+ +FR + VLAGE + ++ F D+ VYWNSRL EH RL
Sbjct: 131 KTIVNKVGTISNEFRVPKFEVLAGENGMETEVKQYGARFKLDYGLVYWNSRLEHEHMRLS 190
Query: 269 SLFKPGQIVGDVFAGVGPFSVPAGKKKVIVLSNDLNPESYKYMQQNIIDNKVGNFVEPLN 328
SLFKPG+ V D+FAG+GPF++PA +K V +NDLNP+S +Y++ N NKV + + N
Sbjct: 191 SLFKPGETVCDMFAGIGPFAIPAAQKGCFVYANDLNPDSVRYLKINAKFNKVDDLICVHN 250
Query: 329 LDGREF----------------IRDSPKLLQQFIER-------------VQGVITVPGGK 359
+D R+F + D+ K + + R I P G
Sbjct: 251 MDARKFFSHLMAVSTCEDNLQSVADNDKTKEAAVSRGGETNSSGEEIRESNASINEPLGA 310
Query: 360 KYKDKVTGET----------------KRTPETKIPIKN--QFFDHYVMNLPDSALQFLDE 401
K T +T KR +T +PI + DH +MNLP SALQFLD
Sbjct: 311 NKKPSGTTKTENGVGKDCKSIEGHANKRLRQTLLPIAKPWEHIDHVIMNLPASALQFLDS 370
Query: 402 FVGLYSRYGFTYEQMVQEHGEQFQTPWIHCHCFHKYDAEEQPEPSMEQLHERVYQQILQI 461
F ++Q+ + P IHC+CF + A E E I
Sbjct: 371 F-----------SNVIQKKYWKGPLPLIHCYCFIR--ASETTE---------------FI 402
Query: 462 MKTTEQELPFN--QFQFHLVRKVAPTKPMFCVSFQLPQS 498
+ E L F+ FH VR VAP K MFC+SF+LP++
Sbjct: 403 IAEAETALKFHIEDPVFHKVRDVAPNKAMFCLSFRLPEA 441
>CE17599_2 [A] KOG2078 tRNA modification enzyme
Length = 442
Score = 156 bits (395), Expect = 6e-38
Identities = 116/355 (32%), Positives = 179/355 (49%), Gaps = 41/355 (11%)
Query: 148 LDYDFWKVEEILNAILPE--EYLAEIPTGFTIVGHVAHLNLRKEFKPFGEIIGQVILDKN 205
L ++ W + I A+LP +Y + +T GH+ H N E PF II +V+LDK
Sbjct: 45 LTFENWDAKSIFKAVLPVGIDY-----SSYTQTGHIIHCNFADEILPFRFIIAEVLLDKV 99
Query: 206 STIKTVVDKVDSIATKFRTFEMNVLAGEPNLLVTQRESDCSFTFDFSKVYWNSRLHTEHA 265
+ KTVV K + I +R ++ +LAGE N + +E+ F DFSKVYWNSRL EH
Sbjct: 100 NNCKTVVQKGNIITNVYRNLDLELLAGEDNYVTEVKETGLRFKMDFSKVYWNSRLSHEHE 159
Query: 266 RLVSLFKPGQIVGDVFAGVGPFSVPA--GKKKVIVLSNDLNPESYKYMQQNIIDNKV-GN 322
R+ +F +V D G+GPF +PA +K V++NDLNPES K+++ N+ NK+
Sbjct: 160 RVSGMFNTQSLVYDACCGIGPFVLPATLKRKPKRVVANDLNPESVKWLKVNVGLNKIKEE 219
Query: 323 FVEPLNLDGREFIRDSPKLLQQFIERVQGVITVPGGKKYKDKVTGETKRTPETKIPIKNQ 382
+E N+D + FI+++ + + R+ ++ GE E ++P K
Sbjct: 220 RIEIHNMDAKMFIKEN---VADDVVRLM----------LEESTAGEF----ENEVP-KPM 261
Query: 383 FFDHYVMNLPDSALQFLDEFVGLYSRYGFTYEQMVQEHGEQFQTPWIHCHCFHKYDAEEQ 442
H VMNLP A+ FL F G SR+ E++ + ++ ++C+ F K +
Sbjct: 262 SEVHVVMNLPAYAVNFLPAFRGALSRFKDEIEKVPLDKRYKWN---VYCYLFAKSHV-DV 317
Query: 443 PEPSMEQLHERVYQQILQIMKTT-EQELPFNQFQFHLVRKVAPTKPMFCVSFQLP 496
P+ E R+ + KT E+ L + H VR V+ K MFC +LP
Sbjct: 318 PDSWYEDEARRMCDE-----KTKWERSL---VVKCHNVRTVSSRKEMFCAQLELP 364
>ECU04g0620 [A] KOG2078 tRNA modification enzyme
Length = 360
Score = 140 bits (354), Expect = 3e-33
Identities = 82/205 (40%), Positives = 118/205 (57%), Gaps = 5/205 (2%)
Query: 132 EFLDEQEYELRNHEYVLDYDFWKVEEILNAILPEEYLAEIPTGFTIVGHVAHLNLRKEFK 191
E + EY N E L Y+++ E+L ILPEE + P+ F IVG + HLNL E
Sbjct: 69 EGFSDGEYVPINVELELCYEYFTYSEVLRRILPEE--VQTPSSFEIVGSIVHLNLDGEQM 126
Query: 192 PFGEIIGQVILDKNSTIKTVVDKVDSIATKFRTFEMNVLAGEPNLLVTQRESDCSFTFDF 251
IIG+V+ DK T KTV+ K+ I+ +R+F++ V+ G+P L RE D F D+
Sbjct: 127 KHKNIIGRVVHDK--TGKTVITKIGQISNAYRSFDLEVIGGDPVLETIHREGDILFCIDY 184
Query: 252 SKVYWNSRLHTEHARLVSLFKPGQIVGDVFAGVGPFSVPAGKKKVIVLSNDLNPESYKYM 311
VYW S+L +E A L FK G+++ D F GVGP S+PA KK V SNDLN + + +
Sbjct: 185 RSVYWCSKLQSERAILAGKFKAGEVLCDPFCGVGPVSLPALKKGCRVYSNDLNLRAIECL 244
Query: 312 QQNIIDNKVG-NFVEPLNLDGREFI 335
+++I N++ +E NL EF+
Sbjct: 245 EKSIKINRLDPRNIEIFNLSANEFL 269
>At4g27340 [A] KOG2078 tRNA modification enzyme
Length = 562
Score = 130 bits (326), Expect = 6e-30
Identities = 81/235 (34%), Positives = 127/235 (53%), Gaps = 21/235 (8%)
Query: 114 DSIQDVSQARGKLTSNALEFLDEQEYELRNHEYVLDYDFWKVEEILNAILPEEYLAEIPT 173
+ +QD+ +A L + A +EL L YD+W + EIL A+LP+ + +P+
Sbjct: 285 EEVQDLPEAIKVLFAEAKMADASLSFELVKCRLTLFYDYWPMIEILEAVLPKGMI--VPS 342
Query: 174 GFTIVGHVAHLNLRKEFKPFGEIIGQVILDKNST-IKTVVDKVDSIATKFRTFEMNVLAG 232
F +VGH+AHLNLR E P+ +I +V+LDKN I+TVV+K+D I FRT ++ VLAG
Sbjct: 343 AFEMVGHIAHLNLRDEHLPYKRLIAKVVLDKNQPKIQTVVNKIDPIHNDFRTMQLEVLAG 402
Query: 233 EPNLLVTQRESDCSFTFDFSKV--------------YWNSRLHTEHARLVSLF----KPG 274
+L+ E+ F D ++V W+S + + LF
Sbjct: 403 NHSLVTLVVENGLRFHVDLARVGQIDNTLIVLLLLLVWSSLFCLVSSFVYVLFCLVTYAV 462
Query: 275 QIVGDVFAGVGPFSVPAGKKKVIVLSNDLNPESYKYMQQNIIDNKVGNFVEPLNL 329
+ GDVFAGVGP ++ A + V +NDLNP + ++M+QN + NK+ +E + +
Sbjct: 463 VLTGDVFAGVGPIALAAARIVKRVYANDLNPHAVEFMEQNSVVNKLEKRIERIRI 517
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.321 0.139 0.406
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 30,614,535
Number of Sequences: 60738
Number of extensions: 1374786
Number of successful extensions: 3501
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 3466
Number of HSP's gapped (non-prelim): 12
length of query: 502
length of database: 30,389,216
effective HSP length: 111
effective length of query: 391
effective length of database: 23,647,298
effective search space: 9246093518
effective search space used: 9246093518
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)