ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV3298 good L KOG1982 Replication, recombination and repair Nuclear 5'-3' exoribonuclease-interacting protein, Rai1p
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV3298 1141563 1140412 -384
(384 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YGL246c [L] KOG1982 Nuclear 5'-3' exoribonuclease-interacting pr... 469 e-132
SPAC19D5.06c [L] KOG1982 Nuclear 5'-3' exoribonuclease-interacti... 111 2e-24
Hs20631980 [L] KOG1982 Nuclear 5'-3' exoribonuclease-interacting... 72 2e-12
7293288 [L] KOG1982 Nuclear 5'-3' exoribonuclease-interacting pr... 70 5e-12
At4g17620_2 [L] KOG1982 Nuclear 5'-3' exoribonuclease-interactin... 70 5e-12
CE11044 [L] KOG1982 Nuclear 5'-3' exoribonuclease-interacting pr... 64 5e-10
>YGL246c [L] KOG1982 Nuclear 5'-3' exoribonuclease-interacting protein Rai1p
Length = 387
Score = 469 bits (1206), Expect = e-132
Identities = 228/387 (58%), Positives = 286/387 (72%), Gaps = 4/387 (1%)
Query: 1 MVLSSSLFVQKRSTTTALKQPKELGHYSRTQDNGFLVNDDSRLAYYYLPXXXXXXXXXXX 60
M +S++LFV++R +TTALKQPKE+G YSRT+D +L++DD+ L YYYLP
Sbjct: 1 MGVSANLFVKQRGSTTALKQPKEIGFYSRTKDEEYLISDDTNLNYYYLPDAELDRKLDLS 60
Query: 61 XXXXXFKECNTEEFDSTTLHGLLSTLEEYERRKSKKTKVDIITFRGIIRKLISSAFDSSQ 120
FK+ + D +L GLL T+E ER K KK DIITFRGI RKLIS AFDS
Sbjct: 61 SGFQKFKDYYKDFEDRCSLRGLLETIESSERHKGKKINADIITFRGIARKLISCAFDSPS 120
Query: 121 FNPVNFRIVSFDGQLFIKEV----KTDASETSINKNRESLQARAYYSGYKFEALATLSLP 176
FN V+ RIVSF+GQLFIKEV + ++ R Q ++GYKFE LATLS P
Sbjct: 121 FNTVDLRIVSFNGQLFIKEVPEAVNAAKASSATEAGRNINQDLNVFTGYKFETLATLSNP 180
Query: 177 LPLVPRTTLEKRPKKIINNGDQFISMVRTGIGKCKILIGAEVDCIFDFKEDSDDNLKHYA 236
L PR +EKR K+I+++GD++IS+VRTG+G CK+++GAEVDCIFDFKE+ DNLKHYA
Sbjct: 181 LQYTPREVIEKRTKRIVSHGDEYISVVRTGVGNCKLILGAEVDCIFDFKENGRDNLKHYA 240
Query: 237 ELKCTTMVNTVSDAHKFERKLFKTWLQCFLVGINRIIYGFRDDNFMLKTVEEFTTSEVPL 296
ELKCT V +SD HKFERKLF+TWLQCFLVGI RIIYGF+DD+++LKTVEEF+T EVP+
Sbjct: 241 ELKCTQQVANISDTHKFERKLFRTWLQCFLVGIPRIIYGFKDDHYVLKTVEEFSTEEVPV 300
Query: 297 ILKNNNPQMQTVCVDAIKWYGAFTEWLLTSIPRPEDDIDTIKAYKLIFENNHLKLTEIES 356
+LKNNNPQ+ + C++AIKWYG TEWLL IPR ED I+A+KL+FENNHL+L+EIE
Sbjct: 301 LLKNNNPQVGSACLEAIKWYGLLTEWLLKMIPRDEDPHSQIRAFKLVFENNHLRLSEIEE 360
Query: 357 DDEEYKQLVEGEEILSNSFKQWRKDLR 383
DEEY L++GE ILSN FK+WRK L+
Sbjct: 361 SDEEYSGLIDGEHILSNGFKEWRKSLK 387
>SPAC19D5.06c [L] KOG1982 Nuclear 5'-3' exoribonuclease-interacting protein Rai1p
Length = 352
Score = 111 bits (277), Expect = 2e-24
Identities = 91/306 (29%), Positives = 141/306 (45%), Gaps = 48/306 (15%)
Query: 18 LKQPKELGHYSRTQDNGFLVNDDSRLAYYYLPXXXXXXXXXXXXXXXXFKECNT----EE 73
+ +P E+ YS ++D L+ DDS+L+YYY P F + NT
Sbjct: 18 VSEPLEIACYSLSRDRELLL-DDSKLSYYYPPPL--------------FSDLNTGFPNRF 62
Query: 74 FDSTTLHGLLSTLEEYERRKSKKTKVDIITFRGIIRKLISSAFDSSQFNPVNFRIVSFDG 133
+ +S +++ K + +T+RG+I K++ + D + G
Sbjct: 63 HPPKSDPDPISIVKDVLMTKGIQMNSSFLTWRGLITKIMCAPLDPRNHWETYLVMDPTSG 122
Query: 134 QLFIKEVKTDASETSINKNRESLQARAYYSGYKFEALATLSLPLPLVPRTTLEKRPKKII 193
+ ++E SETS Q R Y GYKFEA++TL R +E+R + +
Sbjct: 123 IIMMEE--RTRSETSYAN-----QDRMCYWGYKFEAISTLPEIWDACSRDQIEQRDNQDV 175
Query: 194 NNGDQFISMVRTGIGKCKILIGAEVDCIFDFKE--------DSDDNL------------K 233
+Q+ S+V+ IGK K+++ EVDCI+D K SDD
Sbjct: 176 VPDEQYCSIVKINIGKSKLILAGEVDCIWDKKPCSAKESDVHSDDGTIEEDASNAENPNL 235
Query: 234 HYAELKCTTMVNTVSDAHKFERKLFKTWLQCFLVGINRIIYGFRDDNFMLKTVEEFTTSE 293
HY ELK T + + +KL K W Q FL+GI RII GFRDDN +L ++E T +
Sbjct: 236 HYVELK--TSKKYPLENYGMRKKLLKYWAQSFLLGIGRIIIGFRDDNGILIEMKELFTHQ 293
Query: 294 VPLILK 299
+P +L+
Sbjct: 294 IPKMLR 299
>Hs20631980 [L] KOG1982 Nuclear 5'-3' exoribonuclease-interacting protein Rai1p
Length = 396
Score = 71.6 bits (174), Expect = 2e-12
Identities = 59/228 (25%), Positives = 107/228 (46%), Gaps = 23/228 (10%)
Query: 101 IITFRGIIRKLISSAFDSSQFNPVNFRIVS--FDGQLFIKEVKTDASETSINKNRESLQA 158
I+T+RG + KL+++ ++ + +++ + F G L++ EV+T + R L
Sbjct: 128 IVTWRGHLTKLLTTPYERQE----GWQLAASRFQGTLYLSEVETPNARAQ-RLARPPLLR 182
Query: 159 RAYYSGYKFEALATLSLPLPLVPRTTLEKRPKKIINNGDQFISMVRTGIGKCKILIGAEV 218
Y GYKFE P P +N F S++R+ +G +L EV
Sbjct: 183 ELMYMGYKFEQYMCADKP-------GSSPDPSGEVNTNVAFCSVLRSRLGSHPLLFSGEV 235
Query: 219 DCIFDFKEDSDDNLKHYAELKCTTMVNTVSDAHKFER-KLFKTWLQCFLVGINRIIYGFR 277
DC D + S Y ELK + +++ F R KL K W Q FL G+ ++ GFR
Sbjct: 236 DCT-DPQAPSTQPPTCYVELKTSKEMHSPGQWRSFYRHKLLKWWAQSFLPGVPNVVAGFR 294
Query: 278 DDNFMLKTVEEFTTSEVPLILKNN----NPQMQTVCVDAIKWYGAFTE 321
+ + + +++ F T ++ ++N+ NP +VC++ + +F +
Sbjct: 295 NPDGFVSSLKTFPTMKMFEYVRNDRDGWNP---SVCMNFCAAFLSFAQ 339
>7293288 [L] KOG1982 Nuclear 5'-3' exoribonuclease-interacting protein Rai1p
Length = 375
Score = 70.1 bits (170), Expect = 5e-12
Identities = 78/300 (26%), Positives = 125/300 (41%), Gaps = 58/300 (19%)
Query: 100 DIITFRGIIRKLISSAFDSSQFNPVNFRIVSFDGQLFIKEVKTDASETSINKNRESLQAR 159
D +T RGI+R+++ +D+ F + +G +++ + +T + N N Q
Sbjct: 116 DFVTLRGILRQIMCLQYDNRSFR---VKATLLNGNVYMCKEETPEQQLE-NANMSRAQRV 171
Query: 160 AYYSGYKFEALATLSL----PLPLVPRTTLEKRPKKIINNGDQFISMVRTGIGKCKILIG 215
G+KFE T + P+ VP +N ++F+ + RT + +L G
Sbjct: 172 MCSWGFKFEQYLTSAQAQGKPVTNVP-----------VNEAEEFMGVYRTNLAGILMLYG 220
Query: 216 AEVDCIFDFKEDSD-------DNLKHYAELKCTTMVNTVSDAHKFERKLFKT---WLQCF 265
AE+DC+ D KE D D+LK + ELK + F K FK+ W Q F
Sbjct: 221 AELDCV-DSKEPVDFKDCRVLDSLK-FVELKTSVFNMNPHQIRTF--KSFKSANWWSQSF 276
Query: 266 LVGINRIIYGFRDDNFMLKTVEEFTTSEVPLILKNNNPQMQTVCVDAIKWYGAFTEWLLT 325
LVGI + G RD ML+ ++E + L N P A+ WY E L
Sbjct: 277 LVGITTLYVGLRDTKGMLQRIDEIDVA----TLARNKPW----SASAMAWY---LEQFLR 325
Query: 326 SIPRPEDDI-DTIKAYKLIFENNHLKLTEIESDDEEYKQLVEGEEILSNSFKQWRKDLRK 384
++ + +I D ++ F N H + + +IL N W +DL K
Sbjct: 326 NLKKLLVNINDPFAVVQVTFLNKHASYEVLRGPE---------HQILPN----WYRDLLK 372
>At4g17620_2 [L] KOG1982 Nuclear 5'-3' exoribonuclease-interacting protein Rai1p
Length = 319
Score = 70.1 bits (170), Expect = 5e-12
Identities = 48/142 (33%), Positives = 73/142 (50%), Gaps = 17/142 (11%)
Query: 198 QFISMVRTGIGKCKILIGAEVDCIFDFKEDSDDNLKHYAELKCTTMVNTVSDAHKFER-K 256
+F S+VRT +G ++++GAE+DC + SD + Y ELK T ++ + +FER K
Sbjct: 150 EFCSVVRTKLGAHRVMMGAEMDCC----DVSDKGKRFYVELKTTRELDDRT-VDRFEREK 204
Query: 257 LFKTWLQCFLVGINRIIYGFRDDNFMLKTVEEFTTSEVPLILKNNNPQMQTVCVDAIKWY 316
L K W+Q F+ G+ I+ GFRDD L E TT ++ + N VC+
Sbjct: 205 LLKFWIQSFVAGVPYIVVGFRDDGGRLVRTERLTTRDIAHRARLKNYWQGGVCL------ 258
Query: 317 GAFTE----WLLTSIPRPEDDI 334
AF + WL ++ ED I
Sbjct: 259 -AFADEVLCWLYGTVKENEDYI 279
>CE11044 [L] KOG1982 Nuclear 5'-3' exoribonuclease-interacting protein Rai1p
Length = 393
Score = 63.5 bits (153), Expect = 5e-10
Identities = 59/213 (27%), Positives = 94/213 (43%), Gaps = 26/213 (12%)
Query: 91 RRKSKKTKVDIITFRGIIRKLISSAFDSSQFNPVNFRI--VSFDGQLFIKEVKTDASETS 148
R K + D++ +RG+I K+ S+ ++ + +RI + G +F+ E KT+ ++
Sbjct: 131 RLKKVIHEADVVCWRGLITKICSTIYNKEE----GWRIDAIKRKGVIFLCEEKTEQAKNR 186
Query: 149 INKNRESLQARAYYSGYKFEALATLS----LPLPLVPRTTLEKRPKKIINN--GDQFISM 202
+ N+ L R Y G+KFE TL P P TT E+ N+ DQ ++
Sbjct: 187 -DLNQTDLDKRMSYWGHKFEQYVTLDDDAEQPDTSSPVTTKEEYAVVFRNDLQTDQRLNP 245
Query: 203 VRTGIGKCKILIGAEVDCIFDFKEDSDDNLKHYAELKCTTMVNTVSDAHKFERKLFKTWL 262
+ IG I EVDC+ +H L+ T + K FK WL
Sbjct: 246 EKRSIG---IFYSGEVDCLD----------RHGNMLELKTQKGELGHGFYKYSKSFKWWL 292
Query: 263 QCFLVGINRIIYGFRDDNFMLKTVEEFTTSEVP 295
Q LV ++ II G R +K++ T E+P
Sbjct: 293 QSSLVNVDHIIVGLRTQEGHVKSLTSLRTREIP 325
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.319 0.136 0.391
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 22,031,459
Number of Sequences: 60738
Number of extensions: 913118
Number of successful extensions: 2599
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 2590
Number of HSP's gapped (non-prelim): 7
length of query: 384
length of database: 30,389,216
effective HSP length: 108
effective length of query: 276
effective length of database: 23,829,512
effective search space: 6576945312
effective search space used: 6576945312
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)