ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV3329 good D KOG0995 Cell cycle control, cell division, chromosome partitioning Centromere-associated protein HEC1
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV3329 1151967 1153985 673
(673 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YIL144w [D] KOG0995 Centromere-associated protein HEC1 468 e-131
SPBC11C11.03 [D] KOG0995 Centromere-associated protein HEC1 130 5e-30
ECU10g0810 [D] KOG0995 Centromere-associated protein HEC1 100 6e-21
Hs5174457 [D] KOG0995 Centromere-associated protein HEC1 88 5e-17
CE06537 [D] KOG0995 Centromere-associated protein HEC1 54 8e-07
Hs8923838 [S] KOG4643 Uncharacterized coiled-coil protein 51 7e-06
>YIL144w [D] KOG0995 Centromere-associated protein HEC1
Length = 691
Score = 468 bits (1203), Expect = e-131
Identities = 252/676 (37%), Positives = 385/676 (56%), Gaps = 15/676 (2%)
Query: 9 TTGPEDVLNSVDPYRYTSQIPNGKSXXXXXXXXXXXXTLTNMINRSIAKNM--------- 59
T+ + VL+ +DP+R+TSQIP S LT+MIN+SIA+N
Sbjct: 5 TSTDQHVLHHMDPHRFTSQIPTATSSQLRRRNSTNQG-LTDMINKSIARNTISGTGIPTG 63
Query: 60 ----GQNAXXXXXXXXXXXXXXXXXPRSSIGPXXXXXXXXXXXXXYHNTARDPRPLRDKN 115
+ +S H + RDPRPLRDKN
Sbjct: 64 GINKNKRTRSTVAGGTNGTALALNDKSNSRNSVSRLSINQLGSLQQHLSNRDPRPLRDKN 123
Query: 116 FQNLLQQEIFSYLTDQKFDVETNHPISLKSLKQPTQKDFIYMFKWLYLRLDPGYVFTKSL 175
FQ+ +Q+EI+ YL KFD+ETNHPIS+K LKQPTQK FI +FKWLYLRLDPGY FTKS+
Sbjct: 124 FQSAIQEEIYDYLKKNKFDIETNHPISIKFLKQPTQKGFIIIFKWLYLRLDPGYGFTKSI 183
Query: 176 EHEVYSILRTIHYPYLATINKSQISAVGGSNWPKFVGMLHWLVIINKKLDECLEHLDVSI 235
E+E+Y IL+ + YP+L +INKSQISAVGGSNW KF+GMLHW+V N KLD CL +D S+
Sbjct: 184 ENEIYQILKNLRYPFLESINKSQISAVGGSNWHKFLGMLHWMVRTNIKLDMCLNKVDRSL 243
Query: 236 NNQVTQDITVLNQPLKTAXXXXXXXXXXXLMVERLFIDYITESYKSFLRLEDDYEPYLHD 295
NQ TQ+IT+L+QPLKT LMVE+L IDY TESYKSFL+LED+YEP + +
Sbjct: 244 INQNTQEITILSQPLKTLDEQDQRQERYELMVEKLLIDYFTESYKSFLKLEDNYEPSMQE 303
Query: 296 LELSFQRFVHIIETDIAQLTSTTEHLSVECESIVAKNYELKNIKEKNTGLEQEYKRLSHG 355
L+L F++FVHII TDIA L + ++L + + ++ + ++K +EK L+ + + +
Sbjct: 304 LKLGFEKFVHIINTDIANLQTQNDNLYEKYQEVMKISQKIKTTREKWKALKSDSNKYENY 363
Query: 356 VQITQAKSKEWPDKLXXXXXXXXXXXXXXXQVSLQIDELTSVLKRKNISGEEIEHKNKES 415
V + KS+EWP KL + I EL +L++K IS E+ E +N+E
Sbjct: 364 VNAMKQKSQEWPGKLEKMKSECELKEEEIKALQSNISELHKILRKKGISTEQFELQNQER 423
Query: 416 DSISKSLDTVSSRLDQFTGLVRQQRLETESVYKNLQDTLKQYKSATEGLVIARKNLGDHI 475
+ +++ LD ++ + D+ T ++ ++LE E ++K+L DTL+QY S+ + L +R LG ++
Sbjct: 424 EKLTRELDKINIQSDKLTSSIKSRKLEAEGIFKSLLDTLRQYDSSIQNLTRSRSQLGHNV 483
Query: 476 DESSIELVLPSDLMSEEM-IGLTVEELIGDKKSFTDPIKKNLNKIAEDIKMRITNIQTEN 534
++SS+++ + +L+ + G++ E+L + IKK++ K+ ++I+ RI I+ +N
Sbjct: 484 NDSSLKINISENLLDRDFHEGISYEQLFPKGSGINESIKKSILKLNDEIQERIKTIEKDN 543
Query: 535 SKLQIKLEDLRNEITTKNEDLDQLEQQLSSIKXXXXXXXXXXXXXXXXXNIKIEKLERKI 594
L+ +++L+++I K + ++LE +LS I+IEK+E+KI
Sbjct: 544 ITLEKDIKNLKHDINEKTQINEKLELELSEANSKFELSKQENERLLVAQRIEIEKMEKKI 603
Query: 595 QNARVESQQKISSIXXXXXXXXXXXXXXXXXXXXXXXXXHNKVIQLIEYVSNFKINTQSS 654
++ + + KIS H +VI +I+ S FKIN QSS
Sbjct: 604 NDSNLLMKTKISDAEELVTSTELKLEELKVDLNRKRYKLHQQVIHVIDITSKFKINIQSS 663
Query: 655 IEELQNATQKELQRLQ 670
+E +N ++ L+
Sbjct: 664 LENSENELGNVIEELR 679
>SPBC11C11.03 [D] KOG0995 Centromere-associated protein HEC1
Length = 624
Score = 130 bits (328), Expect = 5e-30
Identities = 109/565 (19%), Positives = 242/565 (42%), Gaps = 41/565 (7%)
Query: 106 RDPRPLRDKNFQNLLQQEIFSYLTDQKFDVETNHPISLKSLKQPTQKDFIYMFKWLYLRL 165
+DPRPL D+ +Q ++ +YL + F + P+ L + P+ ++F +FK LY +L
Sbjct: 95 KDPRPLSDRRYQQECATQVVNYLLESGF----SQPLGLNNRFMPSTREFAAIFKHLYNKL 150
Query: 166 DPGYVFTKSLEHEVYSILRTIHYPYLATINKSQISAVGGSN-WPKFVGMLHWLVIINKKL 224
DP + F E +V + L+ ++YP+L +I++S++ A+G + WP +GMLHW+V L
Sbjct: 151 DPNFRFGARYEEDVTTCLKALNYPFLDSISRSRLVAIGSPHVWPAILGMLHWVV----SL 206
Query: 225 DECLEHLDVSINNQVTQDITVLNQPLKTAXXXXXXXXXXXLMVERLFIDYITESYKSFLR 284
+C E V TV L +V+++ DY+ +Y +L
Sbjct: 207 IQCTE-------KAVAMVYTVEQNSLDDH------------LVDKVLFDYLVRTYHLYLD 247
Query: 285 LEDDYEPYLHDLELSFQRFVHIIETDIAQLTSTTEHLSVECESIVAKNYELKNIKEKNTG 344
+ +L+ +F + + L ST E L + +S + ++ ++E+
Sbjct: 248 ESPEESEPEKELKATFNQQNQDLYNQTEALKSTNEELINQIKSAEELDSAIQVLEERYRT 307
Query: 345 LEQEYKRLSHGVQITQAKSKEWPDKLXXXXXXXXXXXXXXXQVSLQIDELTSVLKRKNIS 404
++++ + + ++K + + + + + D L ++ ++IS
Sbjct: 308 MQRDEVKFQSAMSGMKSKMESRTNLMKQLQVNIEEKESQLQLLKEKRDSLKYQVENQDIS 367
Query: 405 GEEIEHKNKESDSISKSLDTVSSRLDQFTGLVRQQRLETESVYKNLQDTLKQYKSATEGL 464
E E E + + ++L+ + S++ + V L ++ +L+ ++++ S +
Sbjct: 368 ISEFEKMVSEREQLDRNLNMIGSKISELRKEVFDTDLLIQASIDSLEKKVQKFNSLAYRI 427
Query: 465 VIARKNLGDHIDESSIELVLPSDLMSEEMIGLTVEELIGDKKSFTDPIKKNLNKIAEDIK 524
I I ++ + L + I L ++ + ++ +N++ I
Sbjct: 428 GIVPIAA---IRSANNDFELEINPEGPNYINLDLK----------NKVRPFINEVRRSIT 474
Query: 525 MRITNIQTENSKLQIKLEDLRNEITTKNEDLDQLEQQLSSIKXXXXXXXXXXXXXXXXXN 584
+ Q ++ KLQ ++ + + I ++L +E +L+S+ +
Sbjct: 475 LEFHEEQNKSLKLQEHVDTVNDLIAELQDELRGIESRLTSVLSECNMLRETASEEKNAFD 534
Query: 585 IKIEKLERKIQNARVESQQKISSIXXXXXXXXXXXXXXXXXXXXXXXXXHNKVIQLIEYV 644
+ +KLER++Q ++ S + + + +V ++ +
Sbjct: 535 AESDKLERELQQLKLSSHNSMLQLDQRIQSINIEADQIAHACMEYKNNIYKEVAFVLGEI 594
Query: 645 SNFKINTQSSIEELQNATQKELQRL 669
+FK++ Q S+E+L+ QKEL L
Sbjct: 595 IHFKLHVQDSLEDLKMDYQKELDDL 619
>ECU10g0810 [D] KOG0995 Centromere-associated protein HEC1
Length = 543
Score = 100 bits (250), Expect = 6e-21
Identities = 115/531 (21%), Positives = 216/531 (40%), Gaps = 93/531 (17%)
Query: 106 RDPRPLRDKNFQNLLQQEIFSYLTDQKFDVETNHPISLKSLKQPTQKDFIYMFKWLYLRL 165
RD R +R+K ++ I +L + +D + +S K L P+ KDF +FK+LY +
Sbjct: 40 RDMRVVREKGYRQQCVDGILRFLVENGYDGQ----VSQKILHNPSSKDFQSIFKFLYGFV 95
Query: 166 DPGYVFTKSLEHEVYSILRTIHYPYLATINKSQISAVGGSNWPKFVGMLHWLVIINKKLD 225
D + F+ E EV ++++ + YPY I KSQ+SA+ WP + M WLV
Sbjct: 96 DD-FTFSSRFEDEVVNVMKNLKYPYCGEITKSQLSAITPHTWPVILSMCSWLV------- 147
Query: 226 ECLEHLDVSINNQVTQDITVLNQPLKTAXXXXXXXXXXXLMVERLFIDYITESYKSFLRL 285
+ ++ ++L Q + + VE F +Y+ + Y F+
Sbjct: 148 -----------DLISHGTSLLQQEEERS-------------VESCFFEYVCDGYMRFMEG 183
Query: 286 EDDYEPYLHDLELSFQRFVHIIETDIAQLTSTTEHLSVECESIVAKNYELKNIKEKNTGL 345
++D LE F+R V I T I + + E +++ E + + E L
Sbjct: 184 DEDDTA----LEEEFERRVTEIYTGIFKEIDRKKEELRRIEEMIS---EARKMSEGREEL 236
Query: 346 EQEYKRLSHGVQITQAKSKEWPDKLXXXXXXXXXXXXXXXQVSLQI-------DELTSVL 398
+++ + L+ + + A K+ K +V +I D L + +
Sbjct: 237 DRKKRELTDDLNMLIASEKQLEGKKRKYLGAISRLSEEIVKVEEEIESLRAQEDGLRAQI 296
Query: 399 KRKNISGEEIEHKNKESDSISKSLDTVSSRLDQFTGLVRQQRLETES----------VYK 448
++ I+ E+++ N E + K L+ + + +V +Q E + K
Sbjct: 297 TKQRINPEDVKEMNAEKIELFKELERMKPEKESLMKVVGEQEREAQERAEEVEKLFFDLK 356
Query: 449 NLQD--TLKQYKSATEGLVIARKNLGDHIDESS-------IELVLPSD-LMSEEMIGLTV 498
L+D +L+ K +A + G+ S EL SD L++ EM +
Sbjct: 357 GLRDEISLRIVKDGKGVPRVANEVSGEVFSGMSGVIVRLEEELNSKSDVLVTAEMNKSIL 416
Query: 499 EELIGDKKSFTDPIKKNLNKIAEDIKMRITNIQTENSKLQIKLEDL-RNEITTKNEDLDQ 557
EE G+K+S T + L ++ + M KL ++ +++ NE +++
Sbjct: 417 EEARGEKESITSELNSRLGYYSDKLVMA--------GKLYLEKKEISENEQRKSKTEMEL 468
Query: 558 LEQQLSSIKXXXXXXXXXXXXXXXXXNIKIEKLERKIQNARVESQQKISSI 608
LE +L + N + E+K+Q AR+ + ++SI
Sbjct: 469 LENELLKLN--------------LESNTSLLMSEQKLQKARIVLDRTLNSI 505
>Hs5174457 [D] KOG0995 Centromere-associated protein HEC1
Length = 642
Score = 87.8 bits (216), Expect = 5e-17
Identities = 101/573 (17%), Positives = 222/573 (38%), Gaps = 42/573 (7%)
Query: 106 RDPRPLRDKNFQNLLQQEIFSYLTDQKFDVETNHPISLKSLKQPTQKDFIYMFKWLYLRL 165
+DPRPL DK F +++ +LT+ + H +S+KSL+ P+ KDF+ +F +LY L
Sbjct: 81 KDPRPLNDKAFIQQCIRQLCEFLTENGYA----HNVSMKSLQAPSVKDFLKIFTFLYGFL 136
Query: 166 DPGYVFTKS-LEHEVYSILRTIHYPYLATINKSQISAVGGSN-WPKFVGMLHWLVIINKK 223
P Y + E EV I + + YP+ ++KS + VG + WP V L WL+
Sbjct: 137 CPSYELPDTKFEEEVPRIFKDLGYPF--ALSKSSMYTVGAPHTWPHIVAALVWLI----- 189
Query: 224 LDECLEHLDVSINNQVTQDITVLNQPLKTAXXXXXXXXXXXLMVERLFIDYITESYKSFL 283
+C + I+ + + + PL +M +LF+DY + Y+SF+
Sbjct: 190 --DC-----IKIHTAMKE-----SSPLFDDGQPWGEETEDGIMHNKLFLDYTIKCYESFM 237
Query: 284 RLEDDYEPYLHDLELSFQRFVHIIETDIAQLTSTTEHLSVECESIVAKNYE----LKNIK 339
D ++ +L+ + ++ + L + L+ + + + + L++++
Sbjct: 238 SGADSFDEMNAELQSKLKDLFNVDAFKLESLEAKNRALNEQIARLEQEREKEPNRLESLR 297
Query: 340 EKNTGLEQEYKRLSHGVQITQAKSKEWPDKLXXXXXXXXXXXXXXXQVSLQIDELTSVLK 399
+ L+ + ++ + ++ S KL + + L +++
Sbjct: 298 KLKASLQGDVQKYQAYMSNLESHSAILDQKLNGLNEEIARVELECETIKQENTRLQNIID 357
Query: 400 RKNISGEEIEHKNKESDSISKSLDTVSSRLDQFTGLVRQQRLETESVYKNLQDTLKQYKS 459
+ S +IE N E + + ++++ ++ L+ + + L+ + ++ L +Y
Sbjct: 358 NQKYSVADIERINHERNELQQTINKLTKDLEAEQQKLWNEELKYARGKEAIETQLAEYHK 417
Query: 460 ATEGLVIARKNLGDHIDESSIELVLPSDLMSEEMIGLTVEELIGDKKSFTDPIKKNLNKI 519
L + K ++ E+ + + ++ + + P+K+ LN+
Sbjct: 418 LARKLKLIPKG-AENSKGYDFEIKFNPEAGANCLVKYRAQVYV--------PLKELLNET 468
Query: 520 AEDIKMRITNIQTENSKLQIKLEDLRNEITTKNEDLDQLEQQLSSIKXXXXXXXXXXXXX 579
E+I + + L+ LE L IT + L++++ +
Sbjct: 469 EEEINKAL----NKKMGLEDTLEQLNAMITESKRSVRTLKEEVQKLDDLYQQKIKEAEEE 524
Query: 580 XXXXNIKIEKLERKIQNARVESQQKISSIXXXXXXXXXXXXXXXXXXXXXXXXXHNKVIQ 639
++E LE+ Q +S N + +
Sbjct: 525 DEKCASELESLEKHKHLLESTVNQGLSEAMNELDAVQREYQLVVQTTTEERRKVGNNLQR 584
Query: 640 LIEYVSNFKINTQSSIEELQNATQKELQRLQTE 672
L+E V+ + + +EE +E + +E
Sbjct: 585 LLEMVATHVGSVEKHLEEQIAKVDREYEECMSE 617
>CE06537 [D] KOG0995 Centromere-associated protein HEC1
Length = 590
Score = 53.9 bits (128), Expect = 8e-07
Identities = 90/466 (19%), Positives = 183/466 (38%), Gaps = 59/466 (12%)
Query: 103 NTARDPRPLRDKNFQNLLQQEIFSYLTDQKFDVETNHPISLKSLKQPTQKDFIYMFKWLY 162
N++ PR + K+ +L +I+++L + + ++ P +K + DFI F+ +Y
Sbjct: 57 NSSVAPRDV--KSLVSLNGSKIYNFLVEYE---SSDAPSEQLIMKPRGKNDFIACFELIY 111
Query: 163 LRLDPGYVFTK--SLEHEVYSILRTIHYPYLATINKSQISAVGGSN-WPKFVGMLHWLVI 219
L Y F + +E EV I + + YPY + S +G S+ +P + L WL+
Sbjct: 112 QHLSKDYEFPRHERIEEEVSQIFKGLGYPY--PLKNSYYQPMGSSHGYPHLLDALSWLID 169
Query: 220 INKKLDECLEHLDVSINNQVTQDITVLNQPLKTAXXXXXXXXXXXLMVERLFIDYITESY 279
I + IN+ V++D + + F DY +
Sbjct: 170 I------------IRINSAVSEDTQNILFGDFMEQGKAQEKTLNYAWMTSTFRDYTNDRK 217
Query: 280 KSFLRLEDDYEPYLHDLELSFQRFVHIIETDIAQLTSTTEHLSVECESIVAKNYELKNIK 339
+ ++ H L F++ + E S E L+ EC+ I A ++K
Sbjct: 218 AAENPSSSYWDDTKHRLRKYFEQ-SNEFEDMTKTAASALEMLNYECDEIEADKGNEASLK 276
Query: 340 EKNTGLEQEYKR----LSHGVQITQAKSKEWPDKLXXXXXXXXXXXXXXXQVSLQIDELT 395
E+ + + + ++ L + + Q KE L +V +D+L
Sbjct: 277 EEISRIRDDIRKAKDYLEQNLHVKQHMEKE----LAMVKSEQEEKISENEKVQKMVDDLK 332
Query: 396 SVLKRK----NISGEEIEHKNKESDSISKSLDTVSSRLDQFTGLVRQQRLETESVYKNLQ 451
+ ++ + ++G+E+ N +++ + + + S LD RL E+ +
Sbjct: 333 NKIELQKQIHGLTGKEVRQMNLDNNKDKEVVLEIQSELD---------RLSKETWKLKDE 383
Query: 452 DTLKQYKSATEGLVIARKNLGDHIDESSIELVLPSDL-MSEEMIGLTVEELIGDKKSFTD 510
D K+ KS H+ E ++++ ++ M+ E + E D K + +
Sbjct: 384 DFFKEQKSKF-----------IHLAEQIMKILSGLNIQMNLEPLRAPTNE--RDLKDYWE 430
Query: 511 PIKKN-LNKIAEDIKMRITNIQTENSKLQIKLEDLRNEITTKNEDL 555
+ K + +I+ + R ++TE S+ K I ++E L
Sbjct: 431 TLNKIWVPEISRQLHQRKLELETEQSRFSNKAVTAEERIQIQSETL 476
>Hs8923838 [S] KOG4643 Uncharacterized coiled-coil protein
Length = 742
Score = 50.8 bits (120), Expect = 7e-06
Identities = 57/294 (19%), Positives = 118/294 (39%), Gaps = 32/294 (10%)
Query: 280 KSFLRLEDDYEPYLHDLELSFQRFVHIIETDIAQLTSTTEHLSVECESIVAKNYELKNIK 339
K ++ + E + L + +R + I+E + L T S+ S ++ +K+I+
Sbjct: 454 KDLMKEKAQLEKTIETLRENSERQIKILEQENEHLNQTVS--SLRQRSQISAEARVKDIE 511
Query: 340 EKNTGLEQEYKRLSHGVQITQAKSKEWPDKLXXXXXXXXXXXXXXXQVSLQIDELTSVLK 399
++N L + K S + + + + Q+ +++ +
Sbjct: 512 KENKILHESIKETSSKLSKIEFEKR---------------------QIKKELEHYKEKGE 550
Query: 400 RKNISGEEIEHKNKESDSISKSLDTVSSRLDQFTGLVRQQRLETESVYKNLQDTLKQYKS 459
R E+ H KE++ + K + + ++ L Q+ E E + L+ TL +K+
Sbjct: 551 RAEELENELHHLEKENELLQKKITNLKITCEKIEAL-EQENSELERENRKLKKTLDSFKN 609
Query: 460 ATEGLVIARKNLGDHIDESSIEL-----VLPSDLMSEEMIGLTVEELIGDKKSFTDPIK- 513
T L K +DE ++EL L M + L +EL +K+ ++
Sbjct: 610 LTFQLESLEKE-NSQLDEENLELRRNVESLKCASMKMAQLQLENKELESEKEQLKKGLEL 668
Query: 514 -KNLNKIAEDIKMRITNIQTENSKLQIKLEDLRNEITTKNEDLDQLEQQLSSIK 566
K K E +++ + EN +LQ LE+ +I +L LE + +++
Sbjct: 669 LKASFKKTERLEVSYQGLDIENQRLQKTLENSNKKIQQLESELQDLEMENQTLQ 722
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.313 0.131 0.356
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 30,529,880
Number of Sequences: 60738
Number of extensions: 1117120
Number of successful extensions: 4236
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 4211
Number of HSP's gapped (non-prelim): 14
length of query: 673
length of database: 30,389,216
effective HSP length: 113
effective length of query: 560
effective length of database: 23,525,822
effective search space: 13174460320
effective search space used: 13174460320
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)