ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV3359 good J KOG2529 Translation, ribosomal structure and biogenesis Pseudouridine synthase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV3359 1165197 1166447 417
(417 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YNL292w [J] KOG2529 Pseudouridine synthase 493 e-139
SPBC11C11.10 [J] KOG2529 Pseudouridine synthase 175 1e-43
Hs21040257 [J] KOG2529 Pseudouridine synthase 107 4e-23
At5g14460 [J] KOG2529 Pseudouridine synthase 92 1e-18
Hs7661556 [J] KOG2559 Predicted pseudouridine synthase 64 5e-10
>YNL292w [J] KOG2529 Pseudouridine synthase
Length = 403
Score = 493 bits (1268), Expect = e-139
Identities = 240/354 (67%), Positives = 294/354 (82%), Gaps = 1/354 (0%)
Query: 1 MNGIFAIEKPSGVTSNSFLQQLKNALMKSTIFQKDIQKAVMDRSQQYAKETGKKPSKRKL 60
MNGIFAIEKPSG+TSN F+ +L++AL KS +F K+IQ+A +R QQY K+TGKK SKRKL
Sbjct: 1 MNGIFAIEKPSGITSNQFMLKLQHALTKSQVFSKEIQRATAERKQQYEKQTGKKASKRKL 60
Query: 61 RKVSQIKMGHGGTLDPLASGVMVIGIGSGTKKLSEYLGNCTKIYETEALFGVATTSGDSE 120
RKVS++KMGHGGTLDPLASGV+VIGIG+GTKKL+ YL K+YE+EALFGV+TTSGD E
Sbjct: 61 RKVSKVKMGHGGTLDPLASGVLVIGIGAGTKKLANYLSGTVKVYESEALFGVSTTSGDVE 120
Query: 121 GDIITETSVKHLTWDDLKTVEAKFVGHLKQTPPIYAALKLNGKPLYEYAREGIPLPKAIE 180
G+I+++ SVKHL +DDLKTVE KFVG LKQTPPIYAALK++GKPL+EYAREG PLP+AIE
Sbjct: 121 GEILSQNSVKHLNFDDLKTVEEKFVGQLKQTPPIYAALKMDGKPLHEYAREGKPLPRAIE 180
Query: 181 PRQIQIYSLQLFSDSLTRDHSYPILKLQDVEEREVLKKLNANLMEDKLYFSQEYCEKHGL 240
PRQ+ IY L++FSDSL RDH YP+L+ E + +K LNAN++ D LYFS+EY EKHGL
Sbjct: 181 PRQVTIYDLKVFSDSLKRDHDYPLLRPTTEEAVDTVKNLNANMLNDVLYFSKEYTEKHGL 240
Query: 241 KSEEVEVGNWIPLTDEERASIYSSKNDSYRAPLLHFKTSVSSGTYIRSLISDIGKSMKSS 300
SE +V PL+++E I + DSYRAP LHFK +VSSGTYIRSL+SDIGKSM+SS
Sbjct: 241 DSEVAKVEEPFPLSEQEEQEI-QKEGDSYRAPKLHFKANVSSGTYIRSLVSDIGKSMRSS 299
Query: 301 CYMVKLIRCQQQEWALDKNNVFTLDDFEHNDEEIWSKVLEKVLEEGSKVNIEEE 354
CYMVKLIR QQQ+W+L+KNNVF L DF DE++WSKVLEKVL+EG+ V++ EE
Sbjct: 300 CYMVKLIRLQQQDWSLEKNNVFQLTDFTERDEKVWSKVLEKVLDEGATVDVIEE 353
>SPBC11C11.10 [J] KOG2529 Pseudouridine synthase
Length = 407
Score = 175 bits (443), Expect = 1e-43
Identities = 121/346 (34%), Positives = 179/346 (50%), Gaps = 33/346 (9%)
Query: 3 GIFAIEKPSGVTSNSFLQQLKNALMKSTIFQ--KDIQKAVMDRSQQYAKETGKKPSKRKL 60
G+ AI KPSG TS L +LK + S + Q + DR+++ +RK
Sbjct: 51 GLIAINKPSGRTSAQCLNELKKIISNSELAQYFRPAPPHPNDRNRR----------RRKS 100
Query: 61 RKVSQIKMGHGGTLDPLASGVMVIGIGSGTKKLSEYLGNCTKIYETEALFGVATTSGDSE 120
++ IK+GHGGTLDPLASGV+V+G+G+GTK+LS L +C K Y ALFG +T + DS
Sbjct: 101 NRLPDIKIGHGGTLDPLASGVLVVGLGTGTKQLSSLL-SCMKTYRATALFGCSTDTYDSA 159
Query: 121 GDIITETSVKHLTWDDLKTVEAKFVGHLKQTPPIYAALKLNGKPLYEYAREGIPLPKAIE 180
G II + +V T +++ + F G + Q PP+Y+AL + GK LYEYAREGIPLP++I+
Sbjct: 160 GKII-KIAVHIPTKEEILSGLDAFRGDISQLPPLYSALHIQGKRLYEYAREGIPLPESIK 218
Query: 181 PRQIQIYSLQLFSDSLTRDHSY----PILKLQDVEEREVLKKLNANLMEDKLYFS----- 231
R + L L +H+Y + +E E+L+ + L
Sbjct: 219 ARSMHCEELILKDFIPKEEHTYTDPDEFASKEAIESEELLRPIEGGAERHDLLAKTEQDI 278
Query: 232 --QEYCEKHGLKS------EEVEVGNWIPLTDEERASIYSSKNDSYRA--PLLHFKTSVS 281
Q+ EK KS +V + + + + S A P+ +VS
Sbjct: 279 NPQDGDEKINAKSPTTNSVTDVAKDQTVTNPKKRKFEVTDLARGSRPAIGPIAVLDMTVS 338
Query: 282 SGTYIRSLISDIGKSMKSSCYMVKLIRCQQQEWALDKNNVFTLDDF 327
SG Y+RSLI D+G+ + S +MV L+R +Q +ALD N F +F
Sbjct: 339 SGFYVRSLIHDLGRQVNSEAHMVDLVRLKQGSFALDDENCFDFSEF 384
>Hs21040257 [J] KOG2529 Pseudouridine synthase
Length = 349
Score = 107 bits (266), Expect = 4e-23
Identities = 66/191 (34%), Positives = 98/191 (50%), Gaps = 23/191 (12%)
Query: 1 MNGIFAIEKPSGVTSNSFLQQLKNALMKSTIFQKDIQKAVMDRSQQYAKETGKKPSKRKL 60
++G+FA+ KP G TS L +LK L+ E G +
Sbjct: 68 LSGVFAVHKPKGPTSAELLNRLKEKLLA---------------------EAGMPSPEWTK 106
Query: 61 RKVSQIKMGHGGTLDPLASGVMVIGIGSGTKKLSEYLGNCTKIYETEALFGVATTSGDSE 120
RK +K+GHGGTLD A GV+V+GIGSGTK L+ L +K Y G AT + DS
Sbjct: 107 RKKQTLKIGHGGTLDSAARGVLVVGIGSGTKMLTSMLSG-SKRYTAIGELGKATDTLDST 165
Query: 121 GDIITETSVKHLTWDDLKTVEAKFVGHLKQTPPIYAALKLNGKPLYEYAREGIPLPKAIE 180
G + E +T +D++ + KF G++ Q PP+Y+ALK +G+ L + G + +A
Sbjct: 166 GRVTEEKPYDKITQEDIEGILQKFTGNIMQVPPLYSALKKDGQRLSTLMKRG-EVVEAKP 224
Query: 181 PRQIQIYSLQL 191
R + +YS+ L
Sbjct: 225 ARPVTVYSISL 235
Score = 52.0 bits (123), Expect = 2e-06
Identities = 40/166 (24%), Positives = 78/166 (46%), Gaps = 9/166 (5%)
Query: 196 LTRDHSYPILKLQDVEEREVLKKLNANLMEDKLYFSQEYCEKHGLK-SEEVEVGNWIPLT 254
+T + Y + +D+E +L+K N+M+ +S +K G + S ++ G +
Sbjct: 168 VTEEKPYDKITQEDIEG--ILQKFTGNIMQVPPLYSA--LKKDGQRLSTLMKRGEVVEAK 223
Query: 255 DEERASIYSSKNDSYRAPLLHFKTSVSSGTYIRSLISDIGKSMKSSCYMVKLIRCQQQEW 314
++YS ++ P G YIRSL+SDIGK + S +++L R +Q +
Sbjct: 224 PARPVTVYSISLQKFQPPFFTLDVECGGGFYIRSLVSDIGKELSSCANVLELTRTKQGPF 283
Query: 315 ALDKN----NVFTLDDFEHNDEEIWSKVLEKVLEEGSKVNIEEEFI 356
L+++ + +T+DD + E S ++ + SK E+ +
Sbjct: 284 TLEEHALPEDKWTIDDIAQSLEHCSSLFPAELALKKSKPESNEQVL 329
>At5g14460 [J] KOG2529 Pseudouridine synthase
Length = 540
Score = 92.0 bits (227), Expect = 1e-18
Identities = 47/134 (35%), Positives = 82/134 (61%), Gaps = 4/134 (2%)
Query: 59 KLRKVSQIK-MGHGGTLDPLASGVMVIGIGSGTKKLSEYLGNCTKIYETEALFGVATTSG 117
KLR++ ++K +GH GTLDP+A+G++++ +G TK + Y G K Y G AT++
Sbjct: 340 KLRRLVKVKKVGHAGTLDPMATGLLIVCVGKATKVVDRYQG-MIKGYSGVFRLGEATSTL 398
Query: 118 DSEGDIITETSVKHLTWDDLKTVEAKFVGHLKQTPPIYAALKLNGKPLYEYAREGIPLPK 177
D++ +I S +H+ DD+K F+G + Q PP+++A+K+ G+ +YE AR G +
Sbjct: 399 DADSPVIQRESWEHIKDDDIKKALTSFLGEIWQVPPMFSAIKVGGEKMYEKARRGETV-- 456
Query: 178 AIEPRQIQIYSLQL 191
+ PR+I I+ +
Sbjct: 457 ELSPRRISIFQFDI 470
>Hs7661556 [J] KOG2559 Predicted pseudouridine synthase
Length = 331
Score = 63.5 bits (153), Expect = 5e-10
Identities = 39/122 (31%), Positives = 63/122 (50%), Gaps = 1/122 (0%)
Query: 64 SQIKMGHGGTLDPLASGVMVIGIGSGTKKLSE-YLGNCTKIYETEALFGVATTSGDSEGD 122
+ +K+G G LD ASGV+V+G+G G + L++ Y + TK Y L G AT +G
Sbjct: 87 AHLKVGVGHRLDAQASGVLVLGVGHGCRLLTDMYNAHLTKDYTVRGLLGKATDDFREDGR 146
Query: 123 IITETSVKHLTWDDLKTVEAKFVGHLKQTPPIYAALKLNGKPLYEYAREGIPLPKAIEPR 182
++ +T+ H+T + L + A G ++ +Y+ L L + YE A G+ P P
Sbjct: 147 LVEKTTYDHVTREKLDRILAVIQGSHQKALVMYSNLDLKTQEAYEMAVRGLIRPMNKSPM 206
Query: 183 QI 184
I
Sbjct: 207 LI 208
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.313 0.131 0.367
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 22,523,271
Number of Sequences: 60738
Number of extensions: 933723
Number of successful extensions: 2235
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 2219
Number of HSP's gapped (non-prelim): 8
length of query: 417
length of database: 30,389,216
effective HSP length: 109
effective length of query: 308
effective length of database: 23,768,774
effective search space: 7320782392
effective search space used: 7320782392
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)