ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV3409 good EH KOG2644 Amino acid transport and metabolism 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes
r_klactV3409 good EH KOG2644 Coenzyme transport and metabolism 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV3409 1180828 1179887 -314
(314 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDL045c [EH] KOG2644 3'-phosphoadenosine 5'-phosphosulfate sulfo... 337 1e-92
SPCC1235.04c [EH] KOG2644 3'-phosphoadenosine 5'-phosphosulfate ... 124 1e-28
7292816 [EH] KOG2644 3'-phosphoadenosine 5'-phosphosulfate sulfo... 119 7e-27
CE02303 [EH] KOG2644 3'-phosphoadenosine 5'-phosphosulfate sulfo... 91 2e-18
7298064 [EH] KOG2644 3'-phosphoadenosine 5'-phosphosulfate sulfo... 91 2e-18
At5g03430_2 [EH] KOG2644 3'-phosphoadenosine 5'-phosphosulfate s... 76 7e-14
>YDL045c [EH] KOG2644 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase
(PAPS reductase)/FAD synthetase and related enzymes
Length = 306
Score = 337 bits (865), Expect = 1e-92
Identities = 165/316 (52%), Positives = 217/316 (68%), Gaps = 21/316 (6%)
Query: 2 LQDVALNCYNIVQSYLSIDAESAVATTGDVIKQTQESIQLSRRYLLQDIFTRWDPFNGEI 61
L A CY I SYL ID +S +I TQE+I+L+R+YLL +IF RW P NGEI
Sbjct: 3 LSKAAEMCYEITNSYLHIDQKS------QIIASTQEAIRLTRKYLLSEIFVRWSPLNGEI 56
Query: 62 SFSYNGGKDCQVLLIIYLGCLWEFFLMSIQGSQYDLQFHMFPLQQLPTVYIDQEDTFLTA 121
SFSYNGGKDCQVLL++YL CLWE+F + Q SQ+D +F FP+Q+LPTV+IDQE+TF T
Sbjct: 57 SFSYNGGKDCQVLLLLYLSCLWEYFFIKAQNSQFDFEFQSFPMQRLPTVFIDQEETFPTL 116
Query: 122 QDFLQRSVERYHLSLYESAKDK--QVDMPSAFKSFLSVHPETQAIVIGIRHTDPFGETLQ 179
++F+ + ERY LSLYES + V+M AF+ F+ ++PET+AIVIGIRHTDPFGE L+
Sbjct: 117 ENFVLETSERYCLSLYESQRQSGASVNMADAFRDFIKIYPETEAIVIGIRHTDPFGEALK 176
Query: 180 PIQKTDPSWPQFYRLQPLLHWNLSQIWSFLLYSGEEVCGLYEMGFTSLGSVNNTLRNPYL 239
PIQ+TD +WP F RLQPLLHW+L+ IWSFLLYS E +CGLY GFTS+G +NN+L NP+L
Sbjct: 177 PIQRTDSNWPDFMRLQPLLHWDLTNIWSFLLYSNEPICGLYGKGFTSIGGINNSLPNPHL 236
Query: 240 RIPEPIGSMTKAETEHSDFQWEIENNYRGDS-NMHCNEINENDLNTIKQHDSSEFH---- 294
R + F+WEI + + D+ + IN + ++ + + S++H
Sbjct: 237 R--------KDSNNPALHFEWEIIHAFGKDAEGERSSAINTSPISVVDKERFSKYHDNYY 288
Query: 295 PGWYLTKDEWERAGRL 310
PGWYL D ERAGR+
Sbjct: 289 PGWYLVDDTLERAGRI 304
>SPCC1235.04c [EH] KOG2644 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase
(PAPS reductase)/FAD synthetase and related enzymes
Length = 265
Score = 124 bits (312), Expect = 1e-28
Identities = 70/208 (33%), Positives = 109/208 (51%), Gaps = 17/208 (8%)
Query: 36 QESIQLSRRYLLQDIFTRWDPFNGEISFSYNGGKDCQVLLIIYLGCLWEFFLMSIQGSQY 95
Q + +S R++ + F + P ++ S+NGGKDC VL ++ + L E + Q
Sbjct: 28 QNRLSISLRFI-EYAFETYQP--ERLAMSFNGGKDCLVLFLLCIYYLKEKYKEQAQAK-- 82
Query: 96 DLQFHMFPLQQLPTVYIDQEDTFLTAQDFLQRSVERYHLSLYESAKDKQVDMPSAFKSFL 155
L +P V++ D F DF+ +Y L++ + + + M AF FL
Sbjct: 83 --------LSNIPFVFVRPRDEFPEMDDFVNECQSKYRLNIIKIS----LPMKEAFCKFL 130
Query: 156 SVHPETQAIVIGIRHTDPFGETLQPIQKTDPSWPQFYRLQPLLHWNLSQIWSFLLYSGEE 215
H QAI+IGIR DP G + TD WP+F R+QP+L W+ ++IW LL + +
Sbjct: 131 KEHKHIQAILIGIRRLDPHGLHRIAFEVTDKGWPKFMRIQPILDWSYTEIWDLLLETNTK 190
Query: 216 VCGLYEMGFTSLGSVNNTLRNPYLRIPE 243
C LY+ G+TSLG V++T NP L+ P+
Sbjct: 191 YCSLYDRGYTSLGGVSDTSPNPALKNPD 218
>7292816 [EH] KOG2644 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase
(PAPS reductase)/FAD synthetase and related enzymes
Length = 294
Score = 119 bits (297), Expect = 7e-27
Identities = 74/254 (29%), Positives = 114/254 (44%), Gaps = 49/254 (19%)
Query: 60 EISFSYNGGKDCQVLLIIYLGCLWEFFLMSIQGSQYDLQFHMFPLQQLPTVYIDQEDTFL 119
E+ F +NGGKDC VLL + + L + + S +P +YI D+F
Sbjct: 90 ELIFCFNGGKDCTVLLDLLMRYLRQENISS---------------GDIPMLYIKSGDSFP 134
Query: 120 TAQDFLQRSVERYHLSLYESAKDKQVDMPSAFKSFLSVHPETQAIVIGIRHTDPFGETLQ 179
+F++R V Y + L + + + A S P +A+ +G R+TDP+ + L
Sbjct: 135 EIDEFVERCVRNYRVQLVQY----EGTLKEALTHMSSDMPRIKAVFVGSRNTDPYCQHLA 190
Query: 180 PIQKTDPSWPQFYRLQPLLHWNLSQIWSFLLYSGEEVCGLYEMGFTSLGSVNNTLRNPYL 239
P+Q TD WP RL PLL W+ +W ++ + C LY+ G+TS+G+ NT+ NP+L
Sbjct: 191 PMQPTDNDWPPMMRLNPLLEWSYHDVWHYIHLNSVPYCSLYDRGYTSIGNRANTVPNPHL 250
Query: 240 RIPEPIGSMTKAETEHSDFQWEIENNYRGDSNMHCNEINENDLNTIKQHDSSEFHPGWYL 299
R R D+ C ++ + + D + P W L
Sbjct: 251 R--------------------------RTDAECECG----SNSDAVCSCDLGGYRPAWEL 280
Query: 300 TKDEWERAGRLARK 313
ERAGRL RK
Sbjct: 281 QDATMERAGRLPRK 294
>CE02303 [EH] KOG2644 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase
(PAPS reductase)/FAD synthetase and related enzymes
Length = 519
Score = 90.9 bits (224), Expect = 2e-18
Identities = 63/220 (28%), Positives = 105/220 (47%), Gaps = 30/220 (13%)
Query: 60 EISFSYNGGKDCQVLLIIYLGCLWEFFLMSIQGSQYDLQFHMFPLQQLPTVYIDQEDTFL 119
+I+ S+NGGKDC VLL + + + P + +I ED F
Sbjct: 328 QIALSFNGGKDCTVLLHLL---------------RLKVDEKYGPSTPIQGFHIMVEDQFP 372
Query: 120 TAQDFLQRSVERYHLSLYESAKDKQVDMPSAFKSFLSVHPETQAIVIGIRHTDPFGETLQ 179
A F+ + + Y++ + E + + + K+ S+ P +++G R TDP G+ ++
Sbjct: 373 EATQFIIDAAKFYNIQVLEFPGPLKTGLAALKKTRPSIIP----VLMGSRATDPNGKYMK 428
Query: 180 -PIQKTDPSWPQFYRLQPLLHWNLSQIWSFLLYSGEEVCGLYEMGFTSLGSVNNTLRNPY 238
P++ TD WPQ R+ P+L+W + +W L C LY+ G+TSLG +NT+++P
Sbjct: 429 TPVEWTDSDWPQVLRVCPILNWTYTDVWHMLRGLCVPYCKLYDQGYTSLGGRDNTVKHPA 488
Query: 239 LRIPEPIGSMTKAETEHSDFQWEIENNYRGDSNMHCNEIN 278
LRI G EH +++ N D+ CN N
Sbjct: 489 LRIVSSDG------REHYLPAYKLHN----DAEERCNRSN 518
>7298064 [EH] KOG2644 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase
(PAPS reductase)/FAD synthetase and related enzymes
Length = 254
Score = 90.9 bits (224), Expect = 2e-18
Identities = 61/193 (31%), Positives = 89/193 (45%), Gaps = 28/193 (14%)
Query: 60 EISFSYNGGKDCQVLLIIYLGCLWEFFLMSIQGSQYDLQFHMFPLQQLPTVYIDQEDTFL 119
E+ S+NGGKDC VLL I L P L VY+ + F
Sbjct: 57 EMMLSFNGGKDCTVLLDI-------------------LARMTPPSMPLGAVYVKSANPFE 97
Query: 120 TAQDFLQRSVERYHLSL--YESAKDKQVDMPSAFKSFLSVHPETQAIVIGIRHTDPFGET 177
+ F+ V+RY L L YE + AF+ ++ + +AI +G R +DP
Sbjct: 98 ELEKFVDDCVQRYGLQLRRYEGV------LKVAFEQLIAEDSQVKAIFLGCRRSDPESCN 151
Query: 178 LQPIQKTDPSWPQFYRLQPLLHWNLSQIWSFLLYSGEEVCGLYEMGFTSLGSVNNTLRNP 237
L ++ T+ WP R+ PLL W+ IW++L + C LY+ G+TSLG ++T NP
Sbjct: 152 LYELEPTNNGWPAMMRIFPLLEWSYHDIWNYLRSNNLPYCCLYDQGYTSLGDRSSTRVNP 211
Query: 238 -YLRIPEPIGSMT 249
L E + MT
Sbjct: 212 SLLAYDEKLDKMT 224
>At5g03430_2 [EH] KOG2644 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase
(PAPS reductase)/FAD synthetase and related enzymes
Length = 447
Score = 75.9 bits (185), Expect = 7e-14
Identities = 33/96 (34%), Positives = 55/96 (56%), Gaps = 1/96 (1%)
Query: 146 DMPSAFKSFLSVHPETQAIVIGIRHTDPFGETLQPIQKTDPSWPQFYRLQPLLHWNLSQI 205
D S ++ L +P +AI +G+R DP + + P WP F R+ P+L W+ +
Sbjct: 70 DFKSGLEALLKANP-IRAIFLGVRIGDPTAVGQEQFSPSSPGWPPFMRVNPILDWSYRDV 128
Query: 206 WSFLLYSGEEVCGLYEMGFTSLGSVNNTLRNPYLRI 241
W+FLL + C LY+ G+TS+GS+++T+ N L +
Sbjct: 129 WAFLLTCKVKYCSLYDQGYTSIGSIHDTVPNSLLSV 164
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.320 0.136 0.423
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 19,643,985
Number of Sequences: 60738
Number of extensions: 859980
Number of successful extensions: 2004
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 1985
Number of HSP's gapped (non-prelim): 8
length of query: 314
length of database: 30,389,216
effective HSP length: 106
effective length of query: 208
effective length of database: 23,950,988
effective search space: 4981805504
effective search space used: 4981805504
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)