ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV3495 good J KOG2188 Translation, ribosomal structure and biogenesis Predicted RNA-binding protein, contains Pumilio domains
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV3495 1209218 1211221 668
(668 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YJL010c [J] KOG2188 Predicted RNA-binding protein contains Pumil... 879 0.0
SPAC6G9.02c [J] KOG2188 Predicted RNA-binding protein contains P... 221 2e-57
7304224 [J] KOG2188 Predicted RNA-binding protein contains Pumil... 95 3e-19
Hs20541981 [J] KOG2188 Predicted RNA-binding protein contains Pu... 67 7e-11
At1g72320 [J] KOG2188 Predicted RNA-binding protein contains Pum... 62 3e-09
>YJL010c [J] KOG2188 Predicted RNA-binding protein contains Pumilio domains
Length = 666
Score = 879 bits (2270), Expect = 0.0
Identities = 436/647 (67%), Positives = 536/647 (82%), Gaps = 9/647 (1%)
Query: 3 RVRGRKLIKKLKKDEFHPEDN--KTHDEPTVSHVNETAGGDNSDVSPNTFFGVLDSGELD 60
+ RGR+ K +KDEF P N K H + S N+ A + + P FFGVLD EL+
Sbjct: 5 KTRGRRHQDKQRKDEFEPSSNSAKEHIQQEESTYNDEA--EIKETQPQMFFGVLDREELE 62
Query: 61 YFKKAESTLAIDTFENPDEKYQFINSVIEESKGKELKLATSQISSKLMERIIMESDETQL 120
YFK+AESTL +D FE P+EK+QF+ S+IEE+KGKELKL TSQI+SKLMER+I+E DETQL
Sbjct: 63 YFKQAESTLQLDAFEAPEEKFQFVTSIIEEAKGKELKLVTSQITSKLMERVILECDETQL 122
Query: 121 KTIFKAFNGFYFNLACHKYSSHVLETLLVRGAAQVEKELLTPDFDSVD-EGEFVTTESLF 179
K IF++FNG +F L+CHKY+SHVLETL VR AA VE+ELLTP FD+ + EG +VT E++F
Sbjct: 123 KDIFQSFNGVFFGLSCHKYASHVLETLFVRSAALVERELLTPSFDNNEKEGPYVTMENMF 182
Query: 180 LFLTNELQPNLRFMVSHQYASHVLRILILVLSSKTLPSTVQNNSTLRSKKSKIARKMIDI 239
LF+ NEL+P+L+ M++HQYASHVLR+LIL+LSSKTLP++ + NSTLRSKKSKIARKMIDI
Sbjct: 183 LFMLNELKPHLKTMMNHQYASHVLRLLILILSSKTLPNSTKANSTLRSKKSKIARKMIDI 242
Query: 240 KDNDNYNKIFKTPDSFKVVLRSMLSSIYTSFTHGIPQGSGHFSEISLADVMKFREICVDK 299
KDND++NK+++TP+SFK LR +++++Y FT+G S +IS + + KFRE VDK
Sbjct: 243 KDNDDFNKVYQTPESFKSELRDIITTLYKGFTNGAESRS----DISQSTITKFREYSVDK 298
Query: 300 IGSPVIQLVIQVEGIFDRDRSYWHLIFKDSDEKDPKEESFVEYLLSDAVGSHFLQNVIAS 359
+ SPVIQL+IQVEGIFDRDRS+W L+F +DEKDPKEESF+EYLLSD VGSHFL+NVI S
Sbjct: 299 VASPVIQLIIQVEGIFDRDRSFWRLVFNTADEKDPKEESFLEYLLSDPVGSHFLENVIGS 358
Query: 360 TRIKYVERLYRLYMKDRIPKLAKRDTTAAFVLQSLLKVLNSKEVKEIMDELVPDLSVLLN 419
R+KYVERLYRLYMKDRI KLAKRDTT AFV+++LL+ L K+VK+I+D +VP+LS+LLN
Sbjct: 359 ARLKYVERLYRLYMKDRIVKLAKRDTTGAFVVRALLEHLKEKDVKQILDAVVPELSMLLN 418
Query: 420 SNMDFGTEIIDASIRHDNYLRDEIIEQLMKKYYPRESSDKNILESCLQLSSSTLGNTRDD 479
SNMDFGT II+AS + YLRD++I QL++KYYP +S KNILESCL LS+STLGNTRDD
Sbjct: 419 SNMDFGTAIINASNKQGGYLRDDVIAQLIQKYYPEKSDAKNILESCLLLSASTLGNTRDD 478
Query: 480 WPTAEERRKSLFLEKLIDYDDCFLNSTIDSMLNLPAERLLQMPYHGVFSHVVEHVLQVKR 539
WPTAEERR+S+FLE+LIDYDD FLN TIDSML LP ERL+QM YHGVFSHVVEHVLQ R
Sbjct: 479 WPTAEERRRSVFLEQLIDYDDKFLNITIDSMLALPEERLIQMCYHGVFSHVVEHVLQTTR 538
Query: 540 VAIIKRKLLLNVLCKDIVNLSCNAYGSHIVDKLWTFTAKLAMYKERIATALCADSEKVKN 599
V IIKRK+LLN+L K+ VNL+CN YGSHI+DKLW FTAKL +YKERIA AL ++EKVKN
Sbjct: 539 VDIIKRKMLLNILSKESVNLACNVYGSHIMDKLWEFTAKLTLYKERIARALVLETEKVKN 598
Query: 600 SVYGRQVWKNWSLELFVRKRYDWKKIVKEQELEMFPDSKPLQPKTFK 646
S+YGRQVWKNW LEL+VRK +DWKK++KEQE E+FP+SKPLQPK K
Sbjct: 599 SIYGRQVWKNWKLELYVRKMWDWKKLIKEQEFEIFPNSKPLQPKPEK 645
>SPAC6G9.02c [J] KOG2188 Predicted RNA-binding protein contains Pumilio domains
Length = 655
Score = 221 bits (564), Expect = 2e-57
Identities = 183/659 (27%), Positives = 304/659 (45%), Gaps = 87/659 (13%)
Query: 3 RVRGRKLIKKLKKDEFHPEDNKTHDEPTVSHVNETAGGD----------NSDVSPNTFFG 52
+ RG+K K K++E + + P ++ +E AG D N V P FFG
Sbjct: 6 KTRGKKHSAKQKEEEVNSVVS-----PGIAKNDEGAGNDEGAYQVNRYTNEPVQP--FFG 58
Query: 53 VLDSGELDYFKKAESTLAID-TFENPDEKYQFINSVIEESKGKELKLATSQISSKLMERI 111
LD E YF++AE A T EN D ++ F++S+ E +GKEL + + SK++E++
Sbjct: 59 ALDPEEEKYFQQAEQAFANHFTRENEDTRF-FVDSIYREVQGKELIVCNNVFGSKVLEKL 117
Query: 112 IMESDETQLKTIFKAFNGFYFNLACHKYSSHVLETLLVRGAA--QVEKELLTPDFDSVDE 169
S Q+K+ F A NG Y L + S+ E LL AA +E + +T D D V E
Sbjct: 118 FPLSTSRQIKSFFSALNGHYVELIQTTFGSYAFEKLLSHMAAILNLETQGVTEDQDDVLE 177
Query: 170 GE----FVTTESLFLFLTNELQPNLRFMVSHQYASHVLRILILVLSSKTLPSTVQNNSTL 225
GE F+T E+L +++ NE++P + ++SH+ A HVL + L+L N +T+
Sbjct: 178 GENENVFMTAETLLMYMYNEIRPEVSMLMSHKLAVHVLGKIFLLLDGYRFVYHDGNRTTM 237
Query: 226 RSKKSKIARKMIDIKDNDNYNKIFKTPDSFKVVLRSMLSSIYTSFTHGIPQGSGHFSEIS 285
S P+SF S++ S +
Sbjct: 238 ES---------------------ISVPESFPQFAYSIIDSATDNL--------------- 261
Query: 286 LADVMKFREICVDKIGSPVIQLVIQVEGIFDRDRSYW-----HLIFKD--SDEKDPKEES 338
D + R CVDK S +++ I+++ F+R + L+ K S+E D KE
Sbjct: 262 --DTPELRAYCVDKYASQIMRAFIRID--FERSKKTKKKHDPRLVSKLLLSNEYDLKELP 317
Query: 339 FVEYLLSDAVGSHFLQNVIASTRIKYVERLYRLYMKDRIPKLAKRDTTAAFVLQSLLKVL 398
F+E LL D GS L+ ++ + ++ R Y ++ + R +L A F++Q ++ L
Sbjct: 318 FMETLLKDETGSRILEVIVENMSASHLLRFYAVF-EGRFYRLCVHPI-ANFIMQRYIRRL 375
Query: 399 NSKEVKEIMDELVPDLSVLLNSNMDFGTEIIDASIRHDNYLRDEIIEQLMKKYYPRESSD 458
KE+ ++DEL + ++ + + H + +D+I + D
Sbjct: 376 GRKEIGSVIDELKKNTDNIIRKSFLPVLRTLLEKSNHLHCYQDDIFSMIQTSV-TNSGKD 434
Query: 459 KNILESCLQLSSSTLGNTRDDWPTAEERRKSL-------FLEKLIDYDDCFLNSTIDSML 511
NI+ CL S + RD + RK + LE+++ + + +DS+L
Sbjct: 435 ANIIP-CLLRSK----HKRDKANPENKERKLVNNLLGAQLLEEMLHCEKQHIQLLLDSVL 489
Query: 512 NLPAERLLQMPYHGVFSHVVEHVLQVKRVAIIKRKLLLNVLCKDIVNLSCNAYGSHIVDK 571
+L E++L+ V SH++E +L + + + K LN+L L+ +A GSHIVDK
Sbjct: 490 DLSKEQILEYCLETVSSHLIEGILDLSDLNPVFLKKFLNILSGSFATLAVSAPGSHIVDK 549
Query: 572 LWTFTAKLAMYKERIATALCADSEKVKNSVYGRQVWKNWSLELFVRKRYDWKKIVKEQE 630
W T L +Y+ RI L + VK YG++V NW +ELF R +W + +K+ E
Sbjct: 550 AWKATRALPLYRTRIVRELAEGGDDVKFDFYGKKVCSNWKVELFRRAPDEWYRFMKQDE 608
>7304224 [J] KOG2188 Predicted RNA-binding protein contains Pumilio domains
Length = 665
Score = 95.1 bits (235), Expect = 3e-19
Identities = 139/643 (21%), Positives = 253/643 (38%), Gaps = 77/643 (11%)
Query: 54 LDSGELDYFKKAESTLAIDTFENPDEKYQFINSVIEESKGKELKLATSQISSKLMERIIM 113
+D + +YF + + FE+ +E+ N+V E++ +E+ LA++QI SK +E ++
Sbjct: 38 IDDEQFNYFINILDAMKVG-FEDVEERVIMANNVFEQTHDQEIHLASNQIVSKALESLVG 96
Query: 114 ESDETQLKTIFKAFNGFYFNLACHKYSSHVLETLL----VRG------------------ 151
D+ QL+ F F + +++SHVL+ +L +RG
Sbjct: 97 FVDDVQLERFFSKFGDNLRPMCSDRFASHVLQKMLEIAFLRGVGKSAAQDTSDAASANKK 156
Query: 152 ----AAQVEKEL-LTPDFDSVDEGEFVTTESLFLFLTNELQPNLRFMVSHQYASHVLRIL 206
AAQ E+E L DF D E L ++ + NL V ASH++R
Sbjct: 157 AKPDAAQEEEEYNLQADFTD-DHRE--KCRQFVLRISKFMLNNLEDFVWDACASHIMRTA 213
Query: 207 ILVLSSKTLPSTVQNNSTLRSKKSKIARKMIDIKDNDNYNKIFKTPDSFKVVLRSMLSSI 266
IL L +P ++IA+ + K++ PD ++ V++ +
Sbjct: 214 ILCLVGMHVPKI-----AFEKGGTEIAK----------HRKMYSVPDEWQEVMKEFPQRL 258
Query: 267 YTSFTHGIPQGSGHFSEISLADVMKFREICVDKIGSPVIQLVIQVEGIF---DRDRSYWH 323
G H S SL V+ VDK I G+ D D
Sbjct: 259 EMWPQFGDFPYQDHSS--SLLGVICLALSVVDKSMLKHFAKKILTIGLLKSSDEDEEKME 316
Query: 324 LIFK--------DSDEKDPK-------------EESFVEYLLSDAVGSHFLQNVIASTRI 362
L K D+DEK+ K EE + + L+ +++
Sbjct: 317 LDTKIEIKDEDDDNDEKEDKKAKEADADTENKQEEPKLPKVFHHQSSVILLETIMSVAGA 376
Query: 363 KYVERLYRLYMKDRIPKLAKRDTTAAFVLQSLLKVLNSKEVKEIMDELVPDLSVLLNSNM 422
K + +LY + +RI LA++ T V + L + + + + EL P + LL
Sbjct: 377 KLMIQLYAMLFCNRIGYLARQPGTNFSVQRLLQHIKEVPDFESVFTELQPHVEELLKMGY 436
Query: 423 DFGTEIIDASIRHDNYLRDEIIEQLMKKYYPRESSDKNILESCLQLSSSTLGNTRDDWPT 482
+ A+ + ++I L + +K+ L + D
Sbjct: 437 TGVVSALSAACLRLATKQSQMIAALQSALHVSGDKEKSKLFLTCLIKLKPFEVVTGDESA 496
Query: 483 AEERRKSLFLEKLIDYDDCFLNSTIDSMLNLPAERLLQMPYHGVFSHVVEHVLQVKRVAI 542
SL + L+ ++ ++ +L+LPA +L Q+ SH+V+ +Q K +
Sbjct: 497 FVHLHGSLITQHLLQFNKPIF--LVNCILDLPATQLAQVFNTPNGSHIVDAFMQSKFIGE 554
Query: 543 IKRKLLLNVLCKDIVNLSCNAYGSHIVDKLWTFTAKLAMYKERIATALCADSEKVKNSVY 602
R+ L+ L V+L+ +GS + ++ F A K R+A L + + +K S +
Sbjct: 555 KSRERLIRQLDGFYVDLAITRHGSRVFEQ--CFKAAQDAQKLRMAKELFSKANMLKGSPH 612
Query: 603 GRQVWKNWSLELFVRKRYDWKK-IVKEQELEMFPDSKPLQPKT 644
GR ++ + L+ + W++ + K + E ++K KT
Sbjct: 613 GRIIYTKYRLDTYKLSPTQWQESLSKHLDAEQTKETKRKTAKT 655
>Hs20541981 [J] KOG2188 Predicted RNA-binding protein contains Pumilio domains
Length = 535
Score = 67.4 bits (163), Expect = 7e-11
Identities = 96/429 (22%), Positives = 186/429 (42%), Gaps = 54/429 (12%)
Query: 59 LDYFKKAESTLAIDTFENPDEKYQFINSVIEESKGKELKLATSQISSKLMERIIMESDET 118
L YF++A S L + E +E+ ++++++E + + L L+T++ S++++ ++ S
Sbjct: 62 LGYFRRALSALK-EAPETGEERDLMVHNIMKEVETQALALSTNRTGSEMLQELLGFSPLK 120
Query: 119 QLKTIFKAFNGFYFNLACHKYSSHVLETLLVR-----GAAQVEKELLTPDFDSVDEGEFV 173
L ++ A +ACH+ HVL++ L++ G+A E+E + + +G
Sbjct: 121 PLCRVWAALRSNLRTVACHRCGVHVLQSALLQLPRLLGSAAEEEE---EEEEDGKDGPTE 177
Query: 174 TTESLFLFLTNELQPNLRFMVSHQYASHVLRILILVLSSKTLPSTVQNNSTLRSKKSKIA 233
T E L L L E+ + + S V+R L+ VL L S + R +S A
Sbjct: 178 TLEELVLGLAAEVCDDFLVYCGDTHGSFVVRTLLQVLGGTILES---ERARPRGSQSSEA 234
Query: 234 RK--MIDIKDNDNYNKIFKTPDSFKVVLRSMLSSIYTSFTHGIPQGSGHFSEISLADVMK 291
+K + K D F+ P++F L+ + SS L D+
Sbjct: 235 QKTPAQECKPAD-----FEVPETFLNRLQDLSSSF-------------------LKDIAV 270
Query: 292 FREICVDKIGSPVIQLVIQVEGIFDRDRSYW--HL----IFKDSDEKDPKEESFVEYLLS 345
F DKI S +Q+ +QV R + HL I S + S + L
Sbjct: 271 F---ITDKISSFCLQVALQV---LHRKLPQFCAHLCNAVIGYLSTRGSSVDGSPLLLFLR 324
Query: 346 DAVGSHFLQNVIASTRIKYVERLYRLYMKDRIPKLAKRDTTAAFVLQSLLKVLNSKE-VK 404
D S L+ V+ ++ L+ +++ ++ LA A F LQ LL + + E +
Sbjct: 325 DQTSSRLLEQVLLVLEPPRLQSLFEEHLQGQLQTLAAH-PIANFPLQRLLDAVTTPELLS 383
Query: 405 EIMDELVPDLSVLLNSNMDFGTEIIDASIRHDNYLRDEIIEQLMKKYYPRESSDKNILES 464
+ +EL P L +L + + R + ++++ L++ ++ E S + + +
Sbjct: 384 PVFEELSPVLEAVLAQGHPGVVIALVGACRRVGAYQAKVLQLLLEAFHCAEPSSRQV--A 441
Query: 465 CLQLSSSTL 473
C+ L ++ +
Sbjct: 442 CVPLFATLM 450
>At1g72320 [J] KOG2188 Predicted RNA-binding protein contains Pumilio domains
Length = 753
Score = 62.0 bits (149), Expect = 3e-09
Identities = 114/563 (20%), Positives = 218/563 (38%), Gaps = 56/563 (9%)
Query: 78 DEKYQFINSVIEESKGKELKLATSQISSKLMERIIMESDETQLKTIFKAFNGFYFNLACH 137
+E+ + +EE++G+E ++AT I S +++ ++ + QL + + + +A
Sbjct: 111 EERSVICGNALEETRGREYEIATDYIISHVLQTLLEGCELDQLCSFIRNSASVFPAIAMD 170
Query: 138 KYSSHVLETLLVRGAAQVEKELLTPDFDSVDEGEFVTTESLFLFLTNELQPNLRFMVSHQ 197
+ SHV E+ L A +E PD SV E + + N M+ +
Sbjct: 171 RSGSHVAESALKSLATHLE----NPDAYSV-------IEEALHSICKVIVDNPLDMMCNC 219
Query: 198 YASHVLRILILVLSSKTLPSTVQNNSTLRSKKSKIARKMIDIKDNDNYNKIFKTP-DSFK 256
Y SHVLR L+ + +L S +K SK K +++K + + + P F
Sbjct: 220 YGSHVLRRLLCLCKGVSLDSP----ELYGAKSSKALAKRLNLKMSQLDDNNLEIPHQGFP 275
Query: 257 VVLRSMLSSIYTSFTHGIPQGSGHFSEISLADVMKFREICVDKIGSPVIQLVIQVEGIFD 316
+L +LS + + + MK+ + VD+ S V+Q +++ +
Sbjct: 276 GMLTYLLSGLLS----------------CSREDMKYLQ--VDQYSSLVLQTALRL--MLK 315
Query: 317 RDRSYWHLI--FKDSDEKDPKEESF---------VEYLLSDAVGSHFLQNVIASTRIKYV 365
+D +I + + K E F + + D SH ++ ++
Sbjct: 316 QDEQLLEIIPLILRCNSTNKKVEGFHIETNVAKEILESMKDNSFSHLVEVILEVAPESLY 375
Query: 366 ERLYRLYMKDRIPKLAKRDTTAAFVLQSLLKVLNSKEVKEIM-DELVPDLSVLLNSNMDF 424
++ K+ + +L+ D A FV+Q+L+ +E IM +EL P LL
Sbjct: 376 NEMFNKVFKNSLFELSV-DRCANFVIQALISHARDQEQMGIMWEELAPRFKDLLEQGKSG 434
Query: 425 GTEIIDASIRHDNYLRDEIIEQLMKKYYPRESSDKNILESCLQLSSSTLGNTRDDWPTAE 484
+ A + ++ E L+ S +IL L L + W A
Sbjct: 435 VVASLIAVSQRLQSHENKCCEALVGAVCSTNESRISILPRLLFLDYYFGCRDKSTWEWAP 494
Query: 485 ERRK----SLFLEKLIDYDDCFLNSTIDSMLNLPAERLLQMPYHGVFSHVVEHVLQVKRV 540
+ L L+ + + + I S+ ++ AE + + + V+E L
Sbjct: 495 GAKMHVMGCLILQGIFKFSSDHIQPYITSLTSMKAEYITETAKDSSGARVIEAFLASDAA 554
Query: 541 AIIKRKLLLNVLCKDIVNLSCNAYGSHIVDKLWTFTAKLAMYKERIATALCADSEKVKNS 600
KR+L++ L LS + GS V+K F A +E IA+ L + +
Sbjct: 555 TKQKRRLIIK-LRGHFGELSLHTSGSFTVEK--CFDACNLTLREAIASELLDVKVDLSKT 611
Query: 601 VYGRQVWKNWSLELFVRKRYDWK 623
G + + ++ + + WK
Sbjct: 612 KQGPYLLRKLDIDGYASRPDQWK 634
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.318 0.135 0.381
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 38,459,848
Number of Sequences: 60738
Number of extensions: 1675706
Number of successful extensions: 5219
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 5196
Number of HSP's gapped (non-prelim): 7
length of query: 668
length of database: 30,389,216
effective HSP length: 113
effective length of query: 555
effective length of database: 23,525,822
effective search space: 13056831210
effective search space used: 13056831210
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)