ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV3506 good K KOG3297 Transcription DNA-directed RNA polymerase subunit E'
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV3506 1214440 1215069 210
(210 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YKL144c [K] KOG3297 DNA-directed RNA polymerase subunit E' 350 6e-97
SPBC2G5.07c [K] KOG3297 DNA-directed RNA polymerase subunit E' 186 2e-47
Hs22068810 [K] KOG3297 DNA-directed RNA polymerase subunit E' 165 4e-41
7294695 [K] KOG3297 DNA-directed RNA polymerase subunit E' 161 5e-40
CE06667 [K] KOG3297 DNA-directed RNA polymerase subunit E' 127 1e-29
At1g06790 [K] KOG3297 DNA-directed RNA polymerase subunit E' 104 1e-22
YDR404c [K] KOG3298 DNA-directed RNA polymerase subunit E' 67 2e-11
ECU08g0650 [K] KOG3297 DNA-directed RNA polymerase subunit E' 61 1e-09
Hs4505947 [K] KOG3298 DNA-directed RNA polymerase subunit E' 52 4e-07
>YKL144c [K] KOG3297 DNA-directed RNA polymerase subunit E'
Length = 212
Score = 350 bits (899), Expect = 6e-97
Identities = 161/212 (75%), Positives = 193/212 (90%), Gaps = 2/212 (0%)
Query: 1 MFILTKVADLVRIPPDQFKRDTISTIVHELNTKYANKIIPHIGLCVTVYDVLDVDEGQLK 60
MFIL+K+ADLVRIPPDQF RDTIS I H+LN K+ANKIIP++GLC+T+YD+L V+EGQLK
Sbjct: 1 MFILSKIADLVRIPPDQFHRDTISAITHQLNNKFANKIIPNVGLCITIYDLLTVEEGQLK 60
Query: 61 PGDGAAFINVTFRALVFKPFVGEIVTGWISQCTTEGIKVSLLGLFEDIFVPQNMLFEGSY 120
PGDG+++INVTFRA+VFKPF+GEIVTGWIS+CT EGIKVSLLG+F+DIF+PQNMLFEG Y
Sbjct: 61 PGDGSSYINVTFRAVVFKPFLGEIVTGWISKCTAEGIKVSLLGIFDDIFIPQNMLFEGCY 120
Query: 121 FSPKENAWIWPMDDETKLYFDVNEKIRFRVEQEVFVDVKPKSPKEKELEDQLRAKQEL-- 178
++P+E+AWIWPMD+ETKLYFDVNEKIRFR+E+EVFVDVKPKSPKE+ELE++ + + E+
Sbjct: 121 YTPEESAWIWPMDEETKLYFDVNEKIRFRIEREVFVDVKPKSPKERELEERAQLENEIEG 180
Query: 179 TEEEKRILEKPPAYALLGSCQTDGTGLVSWWE 210
EE EKPPAYALLGSCQTDG GLVSWWE
Sbjct: 181 KNEETPQNEKPPAYALLGSCQTDGMGLVSWWE 212
>SPBC2G5.07c [K] KOG3297 DNA-directed RNA polymerase subunit E'
Length = 203
Score = 186 bits (473), Expect = 2e-47
Identities = 88/215 (40%), Positives = 135/215 (61%), Gaps = 17/215 (7%)
Query: 1 MFILTKVADLVRIPPDQFKRDTISTIVHELNTKYANKIIPHIGLCVTVYDVLDVDEGQLK 60
MF+L++ +D++ I P F + T + E++ KYANK+I +IGL + VYD L + EG +K
Sbjct: 1 MFLLSRFSDIISIHPSNFWKPTKEALAEEIHKKYANKVIQNIGLAICVYDFLKIGEGIIK 60
Query: 61 PGDGAAFINVTFRALVFKPFVGEIVTGWISQCTTEGIKVSLLGLFEDIFVPQNMLFEGSY 120
GDG++++NV FR ++F+PF GE++ G I C+ EGI+V+ + F+DIF+P++MLF+
Sbjct: 61 YGDGSSYMNVVFRLIIFRPFRGEVMLGKIKSCSEEGIRVT-ISFFDDIFIPKDMLFDPCV 119
Query: 121 FSPKENAWIWPMDDE-----TKLYFDVNEKIRFRVEQEVFVDVKPKSPKEKELEDQLRAK 175
F P E AW+W ++ E T+LYFD++E+IRF++E E FVD+ PK R K
Sbjct: 120 FRPDERAWVWKIEGEDGSEGTELYFDIDEEIRFQIESEDFVDISPK-----------RNK 168
Query: 176 QELTEEEKRILEKPPAYALLGSCQTDGTGLVSWWE 210
LE Y L+ SC DG G+ +WW+
Sbjct: 169 NATAITGTEALESVSPYTLIASCSRDGLGIPAWWK 203
>Hs22068810 [K] KOG3297 DNA-directed RNA polymerase subunit E'
Length = 204
Score = 165 bits (418), Expect = 4e-41
Identities = 84/212 (39%), Positives = 121/212 (56%), Gaps = 14/212 (6%)
Query: 1 MFILTKVADLVRIPPDQFKRDTISTIVHELNTKYANKIIPHIGLCVTVYDVLDVDEGQLK 60
MF+L ++ D VRIPP QF+R +I ELN K ANK++ ++GLC+ ++D+ +++ +
Sbjct: 1 MFVLVEMVDTVRIPPWQFERKLNDSIAEELNKKLANKVVYNVGLCICLFDITKLEDAYVF 60
Query: 61 PGDGAAFINVTFRALVFKPFVGEIVTGWISQCTTEGIKVSLLGLFEDIFVPQNMLFEGSY 120
PGDGA+ V FR +VF PF+ EI+ G I C+ EG+ VS LG F+DI +P L + +
Sbjct: 61 PGDGASHTKVHFRCVVFHPFLDEILIGKIKGCSPEGVHVS-LGFFDDILIPPESLQQPAK 119
Query: 121 FSPKENAWIWPMDDET---KLYFDVNEKIRFRVEQEVFVDVKPKSPKEKELEDQLRAKQE 177
F E W+W + E LY D E+IRFRV E FVD P P + +
Sbjct: 120 FDEAEQVWVWEYETEEGAHDLYMDTGEEIRFRVVDESFVDTSPTGP----------SSAD 169
Query: 178 LTEEEKRILEKPPAYALLGSCQTDGTGLVSWW 209
T + + +K Y L+GS G GL+SWW
Sbjct: 170 ATTSSEELPKKEAPYTLVGSISEPGLGLLSWW 201
>7294695 [K] KOG3297 DNA-directed RNA polymerase subunit E'
Length = 240
Score = 161 bits (408), Expect = 5e-40
Identities = 79/214 (36%), Positives = 123/214 (56%), Gaps = 5/214 (2%)
Query: 1 MFILTKVADLVRIPPDQFKRDTISTIVHELNTKYANKIIPHIGLCVTVYDVLDVDEGQLK 60
MF+L ++ D VRI PDQF + + E++ K ANK++ ++GLC+ + D++ + + +
Sbjct: 1 MFVLAELKDNVRIAPDQFHLKLVDAVRDEIDRKLANKVLLNVGLCIALKDIVSLKDSIIL 60
Query: 61 PGDGAAFINVTFRALVFKPFVGEIVTGWISQCTTEGIKVSLLGLFEDIFVPQNMLFEGSY 120
PGDGA+ V FR +VF+P VG ++TG I C+ EG+ V+ LG F+DI +P L S
Sbjct: 61 PGDGASHTEVLFRYVVFRPMVGTVITGKIRNCSREGVHVT-LGFFDDILIPHAALQHPSR 119
Query: 121 FSPKENAWIW--PMDDETK--LYFDVNEKIRFRVEQEVFVDVKPKSPKEKELEDQLRAKQ 176
F E AW+W P++D K L+ DV E I+FRV +E+F + P P + E + Q A
Sbjct: 120 FDEAEQAWVWEYPLEDGAKHDLFMDVGEPIKFRVSREIFEETSPIGPPKTEAQTQQGAST 179
Query: 177 ELTEEEKRILEKPPAYALLGSCQTDGTGLVSWWE 210
E Y ++G+ G G++SWW+
Sbjct: 180 SAAVASATSQEVKTPYRIIGAINESGLGVLSWWD 213
>CE06667 [K] KOG3297 DNA-directed RNA polymerase subunit E'
Length = 239
Score = 127 bits (319), Expect = 1e-29
Identities = 75/214 (35%), Positives = 110/214 (51%), Gaps = 25/214 (11%)
Query: 1 MFILTKVADLVRIPPDQFKRDTISTIVHELNTKYANKIIPHIGLCVTVYDVLDVDEGQLK 60
MFIL+ + D V I P Q D I LN + ANKIIP +GLC+ VYD+ ++ + +
Sbjct: 1 MFILSLLHDTVAIQPHQLSSDQQIVIKKRLNERLANKIIPDLGLCICVYDINEIGDSYIL 60
Query: 61 PGDGAAFINVTFRALVFKPFVGEIVTGWISQCTTEGIKVSLLGLFEDIFVPQNMLFEGSY 120
PG+G V FR +VF+PFV E++ + + +G+ ++ + FEDIFVP L E
Sbjct: 61 PGEGDCRARVKFRMIVFRPFVDEVIEAKVIGSSRQGLCLT-IQFFEDIFVPAEKLPEPHV 119
Query: 121 FSPKENAWIWPMDDE-----TKLYFDVNEKIRFRVEQEVFVDVKPKSPKEKELEDQLRAK 175
F + W W E KLY D + +RFRV + +F D+KP
Sbjct: 120 FEEEGQVWYWEYAQEDGEPPAKLYMDPGKIVRFRVTEIIFKDLKP--------------- 164
Query: 176 QELTEEEKRILEKPPAYALLGSCQTDGTGLVSWW 209
ELT EE++ EK + + G+ + G G + WW
Sbjct: 165 -ELTHEERK-TEK--SMEIKGTMASTGLGCIGWW 194
>At1g06790 [K] KOG3297 DNA-directed RNA polymerase subunit E'
Length = 204
Score = 104 bits (259), Expect = 1e-22
Identities = 64/216 (29%), Positives = 107/216 (48%), Gaps = 27/216 (12%)
Query: 1 MFILTKVADLVRIPPDQFKRDTISTIVHELNTKYANKIIPHIGLCVTVYDVLDVDEGQLK 60
MF L+++ +R+PP I L + +K++ +GLCV++YD+ V+ G +
Sbjct: 1 MFYLSELEHSLRVPPHLLNLPLEDAIKSVLQNVFLDKVLADLGLCVSIYDIKSVEGGFVL 60
Query: 61 PGDGAAFINVTFRALVFKPFVGEIVTGWISQCTTEGIKVSLLGLFEDIFVPQNML----- 115
PGDGAA V R +VF+PFVGE++ + G++++ LG F+DI+VP ++
Sbjct: 61 PGDGAATYKVGLRIVVFRPFVGEVIAAKFKESDANGLRLT-LGFFDDIYVPAPLMPKPNR 119
Query: 116 FEGSYFSPKENAWIWPMDDETKLYF-DVNEKIRFRVEQEVFVDVKPKSPKEKELEDQLRA 174
E ++ K+ W+W + + Y D +I+FRVE + P P E+ + + A
Sbjct: 120 CEPDPYNRKQMIWVWEYGEPKEDYIVDDACQIKFRVESISY----PSVPTERAEDAKPFA 175
Query: 175 KQELTEEEKRILEKPPAYALLGSCQTDGTGLVSWWE 210
+T G+ DG G VSWW+
Sbjct: 176 PMVVT----------------GNMDDDGLGPVSWWD 195
>YDR404c [K] KOG3298 DNA-directed RNA polymerase subunit E'
Length = 171
Score = 66.6 bits (161), Expect = 2e-11
Identities = 35/108 (32%), Positives = 61/108 (56%), Gaps = 5/108 (4%)
Query: 44 LCVTVYDVLDVDEGQLKPGDGAAFINVTFRALVFKPFVGEIVTGWISQCTTEGIKVSLLG 103
LCV YD +D+ G++ P DG+A NV +RA+VFKPF GE+V G + C+ G +V +
Sbjct: 46 LCVLDYDNIDIQRGRILPTDGSAEFNVKYRAVVFKPFKGEVVDGTVVSCSQHGFEVQVGP 105
Query: 104 LFEDIFVPQNMLFEGSYFSPKENAWIWPMDDETKLYFDVNEKIRFRVE 151
+ +FV ++++ + F+ N P ++ + +IR ++E
Sbjct: 106 M--KVFVTKHLMPQDLTFNAGSNP---PSYQSSEDVITIKSRIRVKIE 148
>ECU08g0650 [K] KOG3297 DNA-directed RNA polymerase subunit E'
Length = 176
Score = 61.2 bits (147), Expect = 1e-09
Identities = 46/156 (29%), Positives = 79/156 (50%), Gaps = 12/156 (7%)
Query: 1 MFILTKVADLVRIPPDQFKRDTISTIVHELNTKYANKIIPHIGLCVTVYDVLDVDEGQLK 60
MF ++ D V+I +++ + EL KY +++ +C+ V V++V E K
Sbjct: 1 MFYSAEIEDRVKISLSHLEKE--KEVFEELKRKYVGRMLGDCSICILVARVMEVYE--YK 56
Query: 61 PGDGAAFINVTFRALVFKPFVGEIVTGWISQCTTEGIKVSLLGLFEDIFVPQNML---FE 117
D V F L+FK F GE+V G I + + EG+++ + F +++V ++ L E
Sbjct: 57 VCDEFLIARVWFEVLLFKFFEGEVVCGEILEQSEEGMRLG-VSFFSELWVTKSGLPPATE 115
Query: 118 GSYFS---PKENAWIWPMDDETKLYFDVNEKIRFRV 150
Y + K+ W+W + +KLYF N +RFRV
Sbjct: 116 KVYVNDCGEKQLVWVW-IYRGSKLYFRRNAHVRFRV 150
>Hs4505947 [K] KOG3298 DNA-directed RNA polymerase subunit E'
Length = 172
Score = 52.4 bits (124), Expect = 4e-07
Identities = 36/155 (23%), Positives = 72/155 (46%), Gaps = 13/155 (8%)
Query: 1 MFILTKVADLVRIPPDQFKRDTISTIVHELNTKYANKIIPHIGLCVTVYDVLDVDEGQLK 60
MF + + + P F + ++T+ +L T+ G + V + ++ G ++
Sbjct: 1 MFYHISLEHEILLHPRYFGPNLLNTVKQKLFTEVEGTCTGKYGFVIAVTTIDNIGAGVIQ 60
Query: 61 PGDGAAFINVTFRALVFKPFVGEIVTGWISQCTTEGIKVSLLGLFEDI-----FVPQNML 115
PG G V ++A+VF+PF GE+V ++Q V+ +GLF +I F+ ++ +
Sbjct: 61 PGRGFVLYPVKYKAIVFRPFKGEVVDAVVTQ-------VNKVGLFTEIGPMSCFISRHSI 113
Query: 116 FEGSYFSPKENAWIWPMDDETKLYFDVNEKIRFRV 150
F P N + DE + +++IR ++
Sbjct: 114 PSEMEFDPNSNPPCYKTMDE-DIVIQQDDEIRLKI 147
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.320 0.140 0.424
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,637,154
Number of Sequences: 60738
Number of extensions: 606962
Number of successful extensions: 2794
Number of sequences better than 1.0e-05: 9
Number of HSP's better than 0.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 2772
Number of HSP's gapped (non-prelim): 9
length of query: 210
length of database: 30,389,216
effective HSP length: 102
effective length of query: 108
effective length of database: 24,193,940
effective search space: 2612945520
effective search space used: 2612945520
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)