ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV3531.1 suspect: Pn O KOG1366 Posttranslational modification, protein turnover, chaperones Alpha-macroglobulin
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV3531.1 1222787 1222494 -98
(98 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores:
Score E
Sequences producing significant alignments: (bits) Value
Hs22057712 [O] KOG1366 Alpha-macroglobulin 29 1.3
CE06997 [C] KOG1254 ATP-citrate lyase 28 2.2
Hs4507423 [L] KOG4120 G/T mismatch-specific thymine DNA glycosylase 28 2.8
YPL222w [S] KOG2542 Uncharacterized conserved protein (YdiU family) 26 8.2
7293208 [OT] KOG0045 Cytosolic Ca2+-dependent cysteine protease ... 26 8.2
>Hs22057712 [O] KOG1366 Alpha-macroglobulin
Length = 1857
Score = 28.9 bits (63), Expect = 1.3
Identities = 19/64 (29%), Positives = 27/64 (41%), Gaps = 8/64 (12%)
Query: 7 LTFHVS---RDISLNNYLHQYVSKLSAKTAAHFGRITFENLKSYAPNLALWGGASTFGLF 63
+TFHV+ + NY Q K S G + FEN+ ++ G S FG
Sbjct: 689 MTFHVAVIVTEFGTGNYCMQISEKTSVFITQLLGTVNFENMDTFYRR-----GISYFGTL 743
Query: 64 VFTD 67
F+D
Sbjct: 744 KFSD 747
>CE06997 [C] KOG1254 ATP-citrate lyase
Length = 1106
Score = 28.1 bits (61), Expect = 2.2
Identities = 18/45 (40%), Positives = 24/45 (53%), Gaps = 5/45 (11%)
Query: 23 QYVSKLSAKTAAHFGRITFENLKSYAPNLALWGGASTFGLFVFTD 67
QY+S++ AKT A ++T N K + GGAS VFTD
Sbjct: 262 QYISEMDAKTGASL-KLTILNRKGRVWTMVAGGGASV----VFTD 301
>Hs4507423 [L] KOG4120 G/T mismatch-specific thymine DNA glycosylase
Length = 410
Score = 27.7 bits (60), Expect = 2.8
Identities = 12/36 (33%), Positives = 20/36 (55%)
Query: 28 LSAKTAAHFGRITFENLKSYAPNLALWGGASTFGLF 63
LS+K GRI + L+ Y P +A++ G + +F
Sbjct: 203 LSSKEFREGGRILVQKLQKYQPRIAVFNGKCIYEIF 238
>YPL222w [S] KOG2542 Uncharacterized conserved protein (YdiU family)
Length = 688
Score = 26.2 bits (56), Expect = 8.2
Identities = 16/51 (31%), Positives = 25/51 (48%), Gaps = 1/51 (1%)
Query: 23 QYVSKLSAKTAAHFGRITFENLKSYAPNLALWGGASTFGLFVFTDGW-PTF 72
++V L+A T AH+ F N N ++ G +G F F D + P+F
Sbjct: 312 RHVVSLNANTVAHWQAYGFANGVLNTDNTSIMGLTIDYGPFAFLDKFEPSF 362
>7293208 [OT] KOG0045 Cytosolic Ca2+-dependent cysteine protease (calpain)
large subunit (EF-Hand protein superfamily)
Length = 1043
Score = 26.2 bits (56), Expect = 8.2
Identities = 10/20 (50%), Positives = 13/20 (65%)
Query: 37 GRITFENLKSYAPNLALWGG 56
GRITF+ K++ NL W G
Sbjct: 932 GRITFQQFKTFMVNLKSWQG 951
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.325 0.139 0.448
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,056,207
Number of Sequences: 60738
Number of extensions: 226588
Number of successful extensions: 555
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 552
Number of HSP's gapped (non-prelim): 6
length of query: 98
length of database: 30,389,216
effective HSP length: 74
effective length of query: 24
effective length of database: 25,894,604
effective search space: 621470496
effective search space used: 621470496
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)