ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV3646 good K KOG3341 Transcription RNA polymerase II transcription factor complex subunit
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV3646 1266372 1267067 232
(232 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YPL002c [K] KOG3341 RNA polymerase II transcription factor compl... 286 2e-77
HsM6005755 [K] KOG3341 RNA polymerase II transcription factor co... 147 9e-36
Hs21361380 [K] KOG3341 RNA polymerase II transcription factor co... 147 9e-36
SPBC651.05c [K] KOG3341 RNA polymerase II transcription factor c... 144 8e-35
CE26875 [K] KOG3341 RNA polymerase II transcription factor compl... 122 4e-28
7300783 [K] KOG3341 RNA polymerase II transcription factor compl... 118 6e-27
At4g27040 [K] KOG3341 RNA polymerase II transcription factor com... 108 5e-24
>YPL002c [K] KOG3341 RNA polymerase II transcription factor complex subunit
Length = 233
Score = 286 bits (732), Expect = 2e-77
Identities = 141/231 (61%), Positives = 184/231 (79%), Gaps = 5/231 (2%)
Query: 1 MKKFGVAAFGE--SGQY-DLASNKLGSQNNELNEQLSIFQVKLVEFARQHNKELRDVPRF 57
MK+FG+AAF E G+Y D+ L Q+ EL +QL +FQ +LVEFA++HN EL+ P F
Sbjct: 1 MKQFGLAAFDELKDGKYNDVNKTILEKQSVELRDQLMVFQERLVEFAKKHNSELQASPEF 60
Query: 58 RAKFMRMCSAIGIDPLSIFDKNSHLFHIDDFYYQICVKIIEICRNTKDVNGGVISLEELH 117
R+KFM MCS+IGIDPLS+FD++ HLF ++DFYY++C+K+IEICR TKD+NGGVIS +EL
Sbjct: 61 RSKFMHMCSSIGIDPLSLFDRDKHLFTVNDFYYEVCLKVIEICRQTKDMNGGVISFQELE 120
Query: 118 VNYFKNMNIEMEDIEKAIIMLGPLDGGFELFTIRQKKFLRSVPNELTDDQTKILEICSIM 177
+F+ +N+ ++D+EK+I ML L+ FE+F IR KKFLRSVPNELT DQTKILEICSI+
Sbjct: 121 KVHFRKLNVGLDDLEKSIDMLKSLE-CFEIFQIRGKKFLRSVPNELTSDQTKILEICSIL 179
Query: 178 GYASITLLRANLNWKRVRSQSVLDGMVASGLIWVDSQSLEGEVLYWDPSWI 228
GY+SI+LL+ANL W+ VRS+S LD MVA+GL+W+D Q E LYWDPSWI
Sbjct: 180 GYSSISLLKANLGWEAVRSKSALDEMVANGLLWIDYQG-GAEALYWDPSWI 229
>HsM6005755 [K] KOG3341 RNA polymerase II transcription factor complex subunit
Length = 258
Score = 147 bits (372), Expect = 9e-36
Identities = 84/236 (35%), Positives = 136/236 (57%), Gaps = 12/236 (5%)
Query: 2 KKFGVAAFGESGQYDLASNKLGSQNNELNEQLSIFQVKLVEFARQHNKELRDVPRFRAKF 61
KK A + E G LA ++L ++++QL +F+ L EFA +H +E+R P FR +F
Sbjct: 14 KKLAEAKYKERGTV-LAEDQLA----QMSKQLDMFKTNLEEFASKHKQEIRKNPEFRVQF 68
Query: 62 MRMCSAIGIDPLSIFDKN-SHLFHIDDFYYQICVKIIEICRNTKDVNGGVISLEELHVNY 120
MC+ IG+DPL+ S + + DFYY++ V+IIE+C K NGG+I+LEELH
Sbjct: 69 QDMCATIGVDPLASGKGFWSEMLGVGDFYYELGVQIIEVCLALKHRNGGLITLEELHQQV 128
Query: 121 FKN-----MNIEMEDIEKAIIMLGPLDGGFELFTIRQKKFLRSVPNELTDDQTKILEICS 175
K ++ +D+ +AI L L GF + + ++SVP EL D T +L++
Sbjct: 129 LKGRGKFAQDVSQDDLIRAIKKLKALGTGFGIIPVGGTYLIQSVPAELNMDHTVVLQLAE 188
Query: 176 IMGYASITLLRANLNWKRVRSQSVLDGMVASGLIWVDSQSLEGEVLYWDPSWIINL 231
GY +++ ++A+L W+ R++ VL+ ++ GL W+D Q+ GE YW P+ +L
Sbjct: 189 KNGYVTVSEIKASLKWETERARQVLEHLLKEGLAWLDLQA-PGEAHYWLPALFTDL 243
>Hs21361380 [K] KOG3341 RNA polymerase II transcription factor complex subunit
Length = 258
Score = 147 bits (372), Expect = 9e-36
Identities = 84/236 (35%), Positives = 136/236 (57%), Gaps = 12/236 (5%)
Query: 2 KKFGVAAFGESGQYDLASNKLGSQNNELNEQLSIFQVKLVEFARQHNKELRDVPRFRAKF 61
KK A + E G LA ++L ++++QL +F+ L EFA +H +E+R P FR +F
Sbjct: 14 KKLAEAKYKERGTV-LAEDQLA----QMSKQLDMFKTNLEEFASKHKQEIRKNPEFRVQF 68
Query: 62 MRMCSAIGIDPLSIFDKN-SHLFHIDDFYYQICVKIIEICRNTKDVNGGVISLEELHVNY 120
MC+ IG+DPL+ S + + DFYY++ V+IIE+C K NGG+I+LEELH
Sbjct: 69 QDMCATIGVDPLASGKGFWSEMLGVGDFYYELGVQIIEVCLALKHRNGGLITLEELHQQV 128
Query: 121 FKN-----MNIEMEDIEKAIIMLGPLDGGFELFTIRQKKFLRSVPNELTDDQTKILEICS 175
K ++ +D+ +AI L L GF + + ++SVP EL D T +L++
Sbjct: 129 LKGRGKFAQDVSQDDLIRAIKKLKALGTGFGIIPVGGTYLIQSVPAELNMDHTVVLQLAE 188
Query: 176 IMGYASITLLRANLNWKRVRSQSVLDGMVASGLIWVDSQSLEGEVLYWDPSWIINL 231
GY +++ ++A+L W+ R++ VL+ ++ GL W+D Q+ GE YW P+ +L
Sbjct: 189 KNGYVTVSEIKASLKWETERARQVLEHLLKEGLAWLDLQA-PGEAHYWLPALFTDL 243
>SPBC651.05c [K] KOG3341 RNA polymerase II transcription factor complex subunit
Length = 252
Score = 144 bits (364), Expect = 8e-35
Identities = 85/241 (35%), Positives = 142/241 (58%), Gaps = 14/241 (5%)
Query: 2 KKFGVAAFGESG---QYDLASNKL-GSQNNELNEQLSIFQVKLVEFARQHNKELRDVPRF 57
K+ G+ A + QY+ N+L Q++E+ QLS FQ L FAR+H E++ +F
Sbjct: 3 KRIGIGALNDDEYLKQYEEVGNELIEQQSDEIASQLSTFQEALKTFAREHATEIKQNSQF 62
Query: 58 RAKFMRMCSAIGIDP-LSIFDKNSHL-FHIDDFYYQICVKIIEICRNTKDVNGGVISLEE 115
R F+++ IG+DP +S D+++ +++FYYQ+ V++IE+C T+ NGG++S+ +
Sbjct: 63 RNTFVKLALKIGLDPFVSGSDESAWAAVGMNEFYYQVAVRVIEVCYATQMENGGLLSVSQ 122
Query: 116 L------HVNYFKNMNIEMEDIEKAIIMLGPLDGGFELFTIRQKKFLRSVPNELTDDQTK 169
+ F + + D+ +A+ L PL GF L I K+++RS+P EL DQ
Sbjct: 123 VCRFLNEENEAFGHEWLRETDVVRAVDSLAPLGPGFVLEKIAGKQYIRSLPLELNTDQNV 182
Query: 170 ILEICSIMGYASITLLRANLNWKRVRSQSVLDGMVASGLIWVDSQSLEGEVLYWDPSWII 229
+LE I+GY +I++LR N W+R R VL+ +V+ L+W+DSQ + E+ YW S +I
Sbjct: 183 VLEAVEILGYVTISILRDNYAWERSRCIQVLNDLVSKSLLWIDSQGV--EMAYWGASNLI 240
Query: 230 N 230
+
Sbjct: 241 D 241
>CE26875 [K] KOG3341 RNA polymerase II transcription factor complex subunit
Length = 269
Score = 122 bits (306), Expect = 4e-28
Identities = 68/212 (32%), Positives = 115/212 (54%), Gaps = 9/212 (4%)
Query: 23 GSQNNELNEQLSIFQVKLVEFARQHNKELRDVPRFRAKFMRMCSAIGIDPLSIFDKN-SH 81
G Q + ++QL L FA++H E++ +FR F MC+++G+DPL+ +
Sbjct: 31 GEQLVQFSQQLEQLSAGLEMFAQRHRDEIKKNSQFRRHFQEMCASVGVDPLASSKGFWAK 90
Query: 82 LFHIDDFYYQICVKIIEICRNTKDVNGGVISLEELHVNYFKNMN------IEMEDIEKAI 135
DFYY++ ++I+EIC +T +NGG++++EE+ + + I +DI +A+
Sbjct: 91 ALGFGDFYYELGIQIVEICLSTTHINGGIMTVEEIRNRLMRTRSRTRKDTISTDDILRAV 150
Query: 136 IMLGPLDGGFELFTIRQKKFL-RSVPNELTDDQTKILEICSIMGYASITLLRANLNWKRV 194
L L GFEL + +FL +SVP EL+ D +++L++ Y + L+ L W
Sbjct: 151 DKLKVLGNGFELVPLGGGRFLVQSVPGELSMDHSRVLQLAEDAAYVTKELIIDKLRWDEA 210
Query: 195 RSQSVLDGMVASGLIWVDSQSLEGEVLYWDPS 226
R+ S L+ +V GL W D Q+ + YW PS
Sbjct: 211 RASSALEHLVKEGLAWTDEQASD-TTQYWFPS 241
>7300783 [K] KOG3341 RNA polymerase II transcription factor complex subunit
Length = 254
Score = 118 bits (296), Expect = 6e-27
Identities = 71/221 (32%), Positives = 119/221 (53%), Gaps = 15/221 (6%)
Query: 16 DLASNKLGSQNNELNEQLSIFQVKLVEFARQHNKELRDVPRFRAKFMRMCSAIGIDPLSI 75
DL N+L ++ +Q+ +F+VKL EFA +H +++R +FR +F MC+AIG+DPL+
Sbjct: 26 DLQENQL----EQMTKQMEVFRVKLEEFAMKHKEDIRKNSQFRKQFQEMCAAIGVDPLAT 81
Query: 76 FDKNSHLFHIDDFYYQICVKIIEICRNTKDVNGGVISLEELHVNYFK-------NMNIEM 128
+ + DFYY++ V+++E+C GG++ L++L + I
Sbjct: 82 GKGFWSVLGMGDFYYELGVQVVEVCLAANHKTGGLMELDDLRRRLIAARGQSSVHQEITK 141
Query: 129 EDIEKAIIMLGPLDGGFELFTIRQKKFL-RSVPNELTDDQTKILEICS--IMGYASITLL 185
EDI A L GF + + + K++ +S+P EL+ ++T IL S G + + L
Sbjct: 142 EDILMAAKKLSIFGNGFVVHKLGKGKYIVQSIPGELSMEETNILNAASNTEQGCVTQSQL 201
Query: 186 RANLNWKRVRSQSVLDGMVASGLIWVDSQSLEGEVLYWDPS 226
+L W R+Q LD ++ GL W+D Q+ + E YW PS
Sbjct: 202 IKDLGWTDYRAQQSLDKVLGEGLCWIDKQAGD-EPAYWFPS 241
>At4g27040 [K] KOG3341 RNA polymerase II transcription factor complex subunit
Length = 181
Score = 108 bits (271), Expect = 5e-24
Identities = 64/169 (37%), Positives = 98/169 (57%), Gaps = 8/169 (4%)
Query: 64 MCSAIGIDPLSIFDKN--SHLFHIDDFYYQICVKIIEICRNTKDVNGGVISLEEL--HVN 119
MC+ IG+DPL+ +K + L I DFYY++ V+IIE+C T+ NGG+ISL+EL H+
Sbjct: 1 MCANIGVDPLAS-NKGFWAELLGIGDFYYELGVQIIEVCMLTRSHNGGLISLQELCNHLR 59
Query: 120 YFKNMNIEM---EDIEKAIIMLGPLDGGFELFTIRQKKFLRSVPNELTDDQTKILEICSI 176
+ + E +D +AI L L GFE+ TI +KK +RSVP EL D +ILE+
Sbjct: 60 QRRKKDREAVTEDDCLRAISKLKVLGSGFEVITIGKKKLVRSVPTELNKDHNQILELAQG 119
Query: 177 MGYASITLLRANLNWKRVRSQSVLDGMVASGLIWVDSQSLEGEVLYWDP 225
G+ + ++ L+W R L+ ++ GL +D+ +G+ YW P
Sbjct: 120 QGFVIVEEVQRRLSWTSGRVIDALETLLEEGLAMIDNGHKDGKCRYWFP 168
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.322 0.139 0.410
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,659,879
Number of Sequences: 60738
Number of extensions: 555568
Number of successful extensions: 1411
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 1390
Number of HSP's gapped (non-prelim): 7
length of query: 232
length of database: 30,389,216
effective HSP length: 103
effective length of query: 129
effective length of database: 24,133,202
effective search space: 3113183058
effective search space used: 3113183058
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)