ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV3648 good J KOG2554 Translation, ribosomal structure and biogenesis Pseudouridylate synthase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV3648 1268520 1267189 -444
(444 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YFL001w [J] KOG2554 Pseudouridylate synthase 505 e-143
SPAC25B8.05 [J] KOG2554 Pseudouridylate synthase 324 2e-88
Hs13775234 [J] KOG2554 Pseudouridylate synthase 237 2e-62
7291383 [J] KOG2554 Pseudouridylate synthase 207 2e-53
CE01538 [J] KOG2554 Pseudouridylate synthase 185 1e-46
ECU11g1610 [J] KOG2554 Pseudouridylate synthase 91 4e-18
At1g34150 [J] KOG2554 Pseudouridylate synthase 84 4e-16
At3g06950 [AJ] KOG4393 Predicted pseudouridylate synthase 72 1e-12
Hs13376820 [J] KOG2553 Pseudouridylate synthase 69 1e-11
SPBC887.11 [J] KOG2553 Pseudouridylate synthase 67 4e-11
At2g30320 [J] KOG2553 Pseudouridylate synthase 65 2e-10
CE20158 [J] KOG2553 Pseudouridylate synthase 64 6e-10
At1g20370 [J] KOG2553 Pseudouridylate synthase 62 2e-09
7300636 [J] KOG2553 Pseudouridylate synthase 62 2e-09
At1g76120 [J] KOG2553 Pseudouridylate synthase 59 1e-08
YPL212c [J] KOG2553 Pseudouridylate synthase 56 9e-08
SPCC126.03 [J] KOG2553 Pseudouridylate synthase 53 1e-06
Hs18545392 [AJ] KOG4393 Predicted pseudouridylate synthase 53 1e-06
>YFL001w [J] KOG2554 Pseudouridylate synthase
Length = 442
Score = 505 bits (1300), Expect = e-143
Identities = 254/433 (58%), Positives = 314/433 (71%), Gaps = 10/433 (2%)
Query: 14 GTVSAMSTDQSAYKNWTXXXXXXXXXXXXNTSENGSDAKGNNSDTVAEVKKRSADAAVEA 73
GT + +S S Y NW+ N ++ S+ + D KKR E
Sbjct: 18 GTNAIVSKKDSIYANWSKEQLIRRITELENANKPHSEKFQHIEDN----KKRKISQE-EV 72
Query: 74 ERRKNVKKPRKSREFDFSKYNTRFVAFKFAYLGWNYNGLAIQKDATPLPTVEGTILETMN 133
R K K P+K FDFSK+NTRF+A +FAYLGWNYNGLA+QK+ TPLPTVEGTILE MN
Sbjct: 73 TRSKAKKAPKK---FDFSKHNTRFIALRFAYLGWNYNGLAVQKEYTPLPTVEGTILEAMN 129
Query: 134 KCKLVPSMVPQEYKFSRCGRTDRGVSAMNQVISLRVRSNLTAEEQLDPSYDDKEIQYVNI 193
KCKLVPSMV Q+YKFSRCGRTD+GVSAMNQVISL VRSNLT EEQ DP+ D +EI YV++
Sbjct: 130 KCKLVPSMVLQDYKFSRCGRTDKGVSAMNQVISLEVRSNLTDEEQRDPTNDSREIPYVHV 189
Query: 194 LNQILPEDIRVSAVCLRPPKGFDARFSCKSRHYKYLFSSEGLDLERMQLAAKHYEGEHDF 253
LNQ+LP+DIR+SAVCLRPP FDARFSC RHYKY+F+ + L++E+M AA ++ GE DF
Sbjct: 190 LNQLLPDDIRISAVCLRPPPNFDARFSCVHRHYKYIFNGKNLNIEKMSKAASYFVGERDF 249
Query: 254 RNFCKLDGSKQITNYKRTIKSSNIMHVSEDMYCFDLVGSAFLWHQVRCMVANLFLVGQGL 313
RNFCKLDGSKQITN+KRTI SS I+ +SE YCFDLVGSAFLWHQVRCM+A LFLVGQ L
Sbjct: 250 RNFCKLDGSKQITNFKRTIISSKILPLSETFYCFDLVGSAFLWHQVRCMMAILFLVGQSL 309
Query: 314 EDPNLIKEMLDIEKMPRKPIYEMASDIPLILYECEFSPMEWIVPDQTTDKASRSSSVVNS 373
E P ++ + DIEK P++P+YEMA+DIPL+LY+C+F M+W P KA + ++ +
Sbjct: 310 EVPEIVLRLTDIEKTPQRPVYEMANDIPLLLYDCKFPEMDWQEPTVDDYKAIKFTTATEA 369
Query: 374 VELNYRLKASVAKIFKDNLPRATIKN--KTVINVGDGIGKVVNVYQKMSDRNLMDSFETV 431
+ L+Y LKA+V IFKD LP A N KT+IN+GDG GKVV Y K+ DR++M+ E V
Sbjct: 370 LTLHYELKAAVCNIFKDVLPTANTNNFSKTIINLGDGRGKVVGTYVKLEDRSVMEPVEVV 429
Query: 432 NAKYRERKTRNPK 444
NAKY ++K K
Sbjct: 430 NAKYSKKKNNKNK 442
>SPAC25B8.05 [J] KOG2554 Pseudouridylate synthase
Length = 450
Score = 324 bits (830), Expect = 2e-88
Identities = 188/454 (41%), Positives = 268/454 (58%), Gaps = 49/454 (10%)
Query: 4 LSKLFKLDGK--GTVSAMSTDQSAYKNWTXXXXXXXXXXXXNTSENGSDAKGNNSDTVAE 61
+S++ +LDGK +++ + KN N +NG++ + +
Sbjct: 15 ISRILELDGKLQKNTNSLPNKEIEGKN--------EVIKNKNKLDNGNEIEPQHLSQELV 66
Query: 62 VKKRSADAAVEAERRKNVKKPRKSREFDFSKYNTRFVAFKFAYLGWNYNGLAIQKDATPL 121
++ DA+ ++ K + R F K T FVAFKFAY GWNYNGLA Q + TPL
Sbjct: 67 LQHALQDAS-------SLPKLKPVRPFQLEK--TVFVAFKFAYCGWNYNGLAYQLEPTPL 117
Query: 122 PTVEGTILETMNKCKLVPSMVPQEYKFSRCGRTDRGVSAMNQVISLRVRSNLTAEEQLDP 181
PTVEG + E + + L+ P FSRCGRTD+GVSAM QVISL VR +
Sbjct: 118 PTVEGKVFEALLRAHLITD--PSSCSFSRCGRTDKGVSAMGQVISLNVRHS--------- 166
Query: 182 SYDDKEIQYVNILNQILPEDIRVSAVCLRPPKGFDARFSCKSRHYKYLFSSE----GLDL 237
+ + I Y ++LN++LP DIR++ PP+GFDARFSCK RHYKYLF+ + LD+
Sbjct: 167 --ERRPIIYCDVLNRLLPADIRITGYAF-PPEGFDARFSCKQRHYKYLFTKDYPGGSLDI 223
Query: 238 ERMQLAAKHYEGEHDFRNFCKLDGSKQITNYKRTIKSSNIMHV--SEDMYCFDLVGSAFL 295
+RM AA Y GEHDFRNFCK+D SKQITNY R I SS ++ + S +Y FDL G+AFL
Sbjct: 224 DRMNEAAALYLGEHDFRNFCKIDASKQITNYHRRILSSKVICIDPSTGLYAFDLQGTAFL 283
Query: 296 WHQVRCMVANLFLVGQGLEDPNLIKEMLDIEKMPRKPIYEMASDIPLILYECEFSPMEWI 355
WHQVRCM+A LFL+GQ LE +LI ++LDI+K+P KPIY+MAS+ PLILY+C+F +EW
Sbjct: 284 WHQVRCMMAILFLIGQKLEPASLINDLLDIKKVPTKPIYDMASEYPLILYDCDFDNIEWT 343
Query: 356 VPDQTTDKASRSSSVV--------NSVELNYRLKASVAKIFKDNLPR--ATIKNKTVINV 405
+P + A R + +S+ + ++ + + I N+ R + ++ + +NV
Sbjct: 344 LPTDSPTTAPRIAKHTYETIYHQWHSLRIREQIASFMLDIADHNVKRYGKSEESSSTMNV 403
Query: 406 GDGIGKVVNVYQKMSDRNLMDSFETVNAKYRERK 439
G+G+ K Y + R +S E VN +Y+ +K
Sbjct: 404 GEGLLKRTKKYIPILSRQRQESVEVVNERYKRKK 437
>Hs13775234 [J] KOG2554 Pseudouridylate synthase
Length = 481
Score = 237 bits (605), Expect = 2e-62
Identities = 133/325 (40%), Positives = 191/325 (57%), Gaps = 28/325 (8%)
Query: 59 VAEVKKRSADAAVEAERRKNVKKPRKS-REFDFSKYNTRFVAFKFAYLGWNYNGLAIQKD 117
V +KK A ++ R+N K+ R FDFS + R VA + AY+GW Y G A Q++
Sbjct: 25 VQRLKKEQAKNKEDSNIRENSAGAGKTKRAFDFSAHGRRHVALRIAYMGWGYQGFASQEN 84
Query: 118 ATPLPTVEGTILETMNKCKLVPSMVPQEYKFSRCGRTDRGVSAMNQVISLRVRS------ 171
T+E + E + K +LV S Q + RCGRTD+GVSA QVISL +RS
Sbjct: 85 TNN--TIEEKLFEALTKTRLVESR--QTSNYHRCGRTDKGVSAFGQVISLDLRSQFPRGR 140
Query: 172 ---NLTAEEQLDPSYDDKEIQYVNILNQILPEDIRVSAVCLRPPKGFDARFSCKSRHYKY 228
+ +E+ + + + EI+Y +ILN++LP DIR+ A P F ARFSC R Y+Y
Sbjct: 141 DSEDFNVKEEANAAAE--EIRYTHILNRVLPPDIRILAWAPVEPS-FSARFSCLERTYRY 197
Query: 229 LFSSEGLDLERMQLAAKHYEGEHDFRNFCKLDGSKQITNYKRTIKSSNIMHVSED----- 283
F LD+ M AA+ Y G HDFRN CK+D + + N++RTI S+ + V +
Sbjct: 198 FFPRADLDIVTMDYAAQKYVGTHDFRNLCKMDVANGVINFQRTILSAQVQLVGQSPGEGR 257
Query: 284 ------MYCFDLVGSAFLWHQVRCMVANLFLVGQGLEDPNLIKEMLDIEKMPRKPIYEMA 337
+ F++ G AFL+HQVRCM+A LFL+GQG+E P +I E+L+IEK P+KP Y MA
Sbjct: 258 WQEPFQLCQFEVTGQAFLYHQVRCMMAILFLIGQGMEKPEIIDELLNIEKNPQKPQYSMA 317
Query: 338 SDIPLILYECEFSPMEWIVPDQTTD 362
+ PL+LY+C+F ++WI + +
Sbjct: 318 VEFPLVLYDCKFENVKWIYDQEAQE 342
>7291383 [J] KOG2554 Pseudouridylate synthase
Length = 499
Score = 207 bits (528), Expect = 2e-53
Identities = 119/302 (39%), Positives = 169/302 (55%), Gaps = 21/302 (6%)
Query: 77 KNVKKPRKSREFDFSKYNTRFVAFKFAYLGWNYNGLAIQKDATPLPTVEGTILETMNKCK 136
K K +K R+FD+S + R V K Y GW+Y G A Q+D+ T+E + + +
Sbjct: 80 KTSSKVQKIRKFDWSSAHKRHVLLKITYFGWDYQGFACQEDSND--TIESNLFRALARTC 137
Query: 137 LVPSMVPQEYKFSRCGRTDRGVSAMNQVISLRVRSNLTAEEQLDPSYDDKEIQYVNILNQ 196
L+ S Y RCGRTD+ VSA QVIS+ +RS E QLDP+ EI Y +LN+
Sbjct: 138 LIESRATSNYH--RCGRTDKEVSAFCQVISIDLRSKHPPESQLDPTALSSEIDYCGLLNR 195
Query: 197 ILPEDIR-VSAVCLRPPKGFDARFSCKSRHYKYLFSSEGLDLERMQLAAKHYEGEHDFRN 255
+LP++I+ V+ + LR P + ARF C SR Y+Y F LD+ M+ A DFRN
Sbjct: 196 VLPKNIQCVAWMPLRSPV-YSARFDCVSRTYRYYFPKGDLDIAAMRKACDLLVRHADFRN 254
Query: 256 FCKLDGSKQITNYKRTIKSSNIMHVSE--------DMYCFDLVGSAFLWHQVRCMVANLF 307
FCK+D +TNY R ++S+ + + DMY ++ +AFLWHQ+RC++A L
Sbjct: 255 FCKMDVHNGVTNYMRNLQSARVEACDQTNHTNSGYDMYYLEIQANAFLWHQIRCIMAVLL 314
Query: 308 LVGQGLEDPNLIKEMLDIEKMPRKPIYEMASDIPLILYECEFSPMEWIVPDQTTDKASRS 367
LVGQ E+P +I ++LD+E P KP Y A +PL L+ C+F D TT +
Sbjct: 315 LVGQKKENPGVISDLLDVESNPCKPQYTPAIGLPLNLFRCDFR-------DHTTRSVNHP 367
Query: 368 SS 369
SS
Sbjct: 368 SS 369
>CE01538 [J] KOG2554 Pseudouridylate synthase
Length = 402
Score = 185 bits (469), Expect = 1e-46
Identities = 130/394 (32%), Positives = 204/394 (50%), Gaps = 45/394 (11%)
Query: 78 NVKKPRKSREFDFSKYNTRFVAFKFAYLGWNYNGLAIQKDATPLPTVEGTILETMNKCKL 137
N K +K++ DF + R +A +F YLGW ++GL Q TVE I++ + K L
Sbjct: 12 NSVKSKKAKTLDFLAHPRRKIAIQFFYLGWEHDGLVQQPHTQN--TVENHIMQALIKTHL 69
Query: 138 VPSMVPQEYKFSRCGRTDRGVSAMNQVISLRVRS-----------NLTAEEQ-LDPSYDD 185
+ + FSRCGRTD+GVSA Q ++ VRS + T E Q +D
Sbjct: 70 IEDWTKCD--FSRCGRTDKGVSAFKQTAAMVVRSLCPGDSGVFWSDSTQEHQKVDYKASG 127
Query: 186 KEIQYVNILNQILPEDIRVSAVCLRPPKGFDARFSCKSRHYKYLFSSEGLDLERMQLAAK 245
+E+ YV +LN +LP+ IRV A + F+ARF C R YKY F+ L+LE+M+ A+
Sbjct: 128 EELPYVKMLNGVLPKTIRVFAWA-PVAQTFNARFDCNRRTYKYSFAKADLNLEKMRQGAE 186
Query: 246 HYEGEHDFRNFCKLDGSKQ--ITNYKRTIKSSNIMHVS----EDMYCF---DLVGSAFLW 296
GEHDF NFC++D +++ + +Y R + + VS DMY + GS FLW
Sbjct: 187 LLVGEHDFSNFCQIDMNEKRLLQSYVRKVYEVKVEQVSTHPENDMYSMVELTVSGSGFLW 246
Query: 297 HQVRCMVANLFLVGQGLEDPNLIKEMLDIEKMPRKPIYEMASDIPLILYECEF--SPMEW 354
H +R +V L +G+ E P+LI ++LD++K P +P Y +ASD PL L++C + +EW
Sbjct: 247 HMIRYIVTILQEIGRENEQPSLISQLLDLKKYPSRPQYTLASDTPLCLFDCGYKSEDVEW 306
Query: 355 IVPDQTTDKASRSSSVVNSVELNYRLKASVAKIFKDNLPRATIKNKTVINVGD---GIGK 411
V D T S V ++ + + +++ ++ L T + GD G+ +
Sbjct: 307 KVHDYTL------KSTVTGLQKTWATYQARSRMMENMLGELT--GMAEFSSGDANKGLHE 358
Query: 412 VV------NVYQKMSDRNLMDSFETVNAKYRERK 439
V + Y + +R + +S E+ K E+K
Sbjct: 359 FVQDRPIPSNYIRFENRKMCESLESKKEKMAEKK 392
>ECU11g1610 [J] KOG2554 Pseudouridylate synthase
Length = 339
Score = 90.5 bits (223), Expect = 4e-18
Identities = 74/223 (33%), Positives = 109/223 (48%), Gaps = 24/223 (10%)
Query: 96 RFVAFKFAYLGWNYNGLAIQKDATPLPTVEGTILETMNKCKLVPSMVPQEYKFSRCGRTD 155
R +A K +Y G Y+G++ Q T+ G + + +L +V GRTD
Sbjct: 59 RTIALKISYDGRRYSGVSPQSGRE---TIGGYLEYALKSTRLGEKLV-------YAGRTD 108
Query: 156 RGVSAMNQVISLRVRSNLTAEEQLDPSY-----DDKEIQYVNILNQILPEDIRVSAVCLR 210
GVSA+ + S + S + E + SY D KE +Y +LN LP DIRV V
Sbjct: 109 AGVSAIGMIASGVLTSRI---EMPNRSYAVAEDDYKEYKYDVMLNNHLPPDIRV--VGWA 163
Query: 211 P-PKGFDARFSCKSRHYKYLFSSEGLDLERMQLAAKHYEGEHDFRNFCKLDGSKQITNYK 269
P P F ARF+C R Y+Y F +GLD++RM AA +G F +C S + Y+
Sbjct: 164 PVPDTFSARFTCIQRQYRYYFHKKGLDIDRMSKAASEIKGMSSF--YCFSKHSDKNARYE 221
Query: 270 RTIKSSNIMHVSEDMYCFDLVGSAFLWHQVRCMVANLFLVGQG 312
RT+ ++ D+Y D+ AFL + VR +V + G+G
Sbjct: 222 RTLDECRVVD-DGDLYYLDIRARAFLHNMVRKIVWAIKKSGRG 263
>At1g34150 [J] KOG2554 Pseudouridylate synthase
Length = 393
Score = 84.0 bits (206), Expect = 4e-16
Identities = 84/344 (24%), Positives = 148/344 (42%), Gaps = 61/344 (17%)
Query: 61 EVKK-RSADAAVEAERRKNVKKPRKSREFDFSKYNTRFVAFKFAYLGWNYNGLAIQKDAT 119
EVK RS + RR+ V+K K+ + R+VA K Y G + G + + A
Sbjct: 55 EVKHDRSLSDSSSLVRRRRVRKGDKN-SIPSHLISKRYVALKIMYFGKRFYGFSAE--AQ 111
Query: 120 PLPTVEGTILETMNKCKLVPSMVPQEYKFSRCGRTDRGVSAMNQVISLRVRSNLTAEEQL 179
P++E + + + + +L+ ++ +SRCG R +++ Q
Sbjct: 112 MEPSIESEVFKALERTRLLVGD-KKDSCYSRCG---------------RTDKGVSSTGQF 155
Query: 180 DPSYDDKEIQYVNILNQILPEDIRVSAVCLRP----PKGFDARFSCKSRHYKYLFSSEGL 235
+ + + N + P +V A+ LR P G D++ R + E
Sbjct: 156 KNQFLIVLMFFQNPVMGAFPNVYKVIALFLRSRLKSPPG-DSKAQVNGR------TGERP 208
Query: 236 DLERMQLAAKHYEGEHDFRNFCKLDGSKQITNYKRTIKSSNIMHVSEDMYCFDLVGSAFL 295
+ + +++ + L ++ + + H + + F + GSAFL
Sbjct: 209 EYDYVRVLNR------------ALPDDIRVIGWSPAPIDFHASHEGDQLCTFTMRGSAFL 256
Query: 296 WHQVRCMVANLFLVGQGLED---------------PNLIKEMLDIEKMPRKPIYEMASDI 340
WHQ+RCMVA LF++GQG+E +I +LD +K PRKP Y +AS+I
Sbjct: 257 WHQIRCMVAVLFMIGQGVESVDVSHLETFMDELAFTQVIDTLLDTKKTPRKPQYLLASEI 316
Query: 341 PLILYECEFSPMEWIVPDQTTDKASRSSSVVNSVELNYRLKASV 384
PL+L CEF +++I + A S + L Y+L++ +
Sbjct: 317 PLVLRTCEFENVDFIC---SPGAAESLRSHFKNESLTYQLESVI 357
>At3g06950 [AJ] KOG4393 Predicted pseudouridylate synthase
Length = 298
Score = 72.4 bits (176), Expect = 1e-12
Identities = 65/252 (25%), Positives = 111/252 (43%), Gaps = 38/252 (15%)
Query: 89 DFSKYNTRFVAFKFAYLGWNYNGLAIQKDATPLPTVEGTILETMNKCKLVPSMVPQEYKF 148
D Y R V AY G + G Q+ PT++ + + + + + + +E +
Sbjct: 12 DSGAYKWRLV---IAYDGTRFAGWQYQESP---PTIQSMLEKALIQ---ITELGRKELQL 62
Query: 149 SRCGRTDRGVSAMNQVISLRVRSNLTAEEQLDPSYDDKEIQYVNILNQILPEDIRVSAVC 208
GRTD GV A QV N T+ + + LN +LP+DIRV +
Sbjct: 63 IGAGRTDAGVHAWGQVAHFVTPFNYTSLDSFHAA-----------LNGLLPKDIRVRELS 111
Query: 209 LRPPKGFDARFSCKSRHYKYLFSSEG----------------LDLERMQLAAKHYEGEHD 252
P+ F ARFS S+ Y+Y ++ L+ +M+ AA + G+HD
Sbjct: 112 AAVPE-FHARFSASSKVYRYQIYNDTFMDPFQRHWAYHCAYKLNASKMREAANLFVGKHD 170
Query: 253 FRNFCKLDGSKQITNYKRTIKSSNIMHVSEDMYCFDLVGSAFLWHQVRCMVANLFLVGQG 312
F F + + +TI +++ + + ++ GS FL+ QVR MVA L +G+
Sbjct: 171 FSAFANATREDGVPDPLKTISRFDVIQMGS-LLQLEVEGSGFLYRQVRNMVALLIQIGKE 229
Query: 313 LEDPNLIKEMLD 324
D +++ +L+
Sbjct: 230 ALDSDIVPMILE 241
>Hs13376820 [J] KOG2553 Pseudouridylate synthase
Length = 399
Score = 68.9 bits (167), Expect = 1e-11
Identities = 63/262 (24%), Positives = 116/262 (44%), Gaps = 44/262 (16%)
Query: 96 RFVAFKFAYLGWNYNGLAIQKDATPLPTVEGTILETMNKCKLVPSMVPQEYK---FSRCG 152
R + AY G Y+G+ ++ T+E ++ + + +P ++ + F RC
Sbjct: 56 RKIVLLMAYSGKGYHGMQRNVGSSQFKTIEDDLVSALVRSGCIPENHGEDMRKMSFQRCA 115
Query: 153 RTDRGVSAMNQVISLRVRSNLTAEEQLDPSYDDKEIQYVNILNQILPEDIRVSAVCLRPP 212
RTD+GVSA QV+SL+V DD + +N LP IR+ + R
Sbjct: 116 RTDKGVSAAGQVVSLKVW-----------LIDD----ILEKINSHLPSHIRILGL-KRVT 159
Query: 213 KGFDARFSCKSRHYKYL-----FSSEGLDL--ERMQLAAKH----------YEGEHDFRN 255
GF+++ C +R Y YL F+ + D+ E +L+A+ Y+G H+F N
Sbjct: 160 GGFNSKNRCDARTYCYLLPTFAFAHKDRDVQDETYRLSAETLQQVNRLLACYKGTHNFHN 219
Query: 256 FCKLDGSKQITNYKRTIK---SSNIMHVSEDMYCFDLVGSAFLWHQVRCMVANLFLVGQG 312
F G + + + ++ + + + G +F+ HQ+R MV + + +G
Sbjct: 220 FTSQKGPQDPSACRYILEMYCEEPFVREGLEFAVIRVKGQSFMMHQIRKMVGLVVAIVKG 279
Query: 313 LEDPNLIK-----EMLDIEKMP 329
++++ E +D+ K P
Sbjct: 280 YAPESVLERSWGTEKVDVPKAP 301
>SPBC887.11 [J] KOG2553 Pseudouridylate synthase
Length = 451
Score = 67.4 bits (163), Expect = 4e-11
Identities = 52/175 (29%), Positives = 84/175 (47%), Gaps = 16/175 (9%)
Query: 63 KKRSADAAVEAERRKNVKKPRKSREFDFSKYNTRFVAFKFAYLGWNYNGLAIQKDATPLP 122
K+++ + AE + K P++ + + N + Y G+ Y+G+ I PL
Sbjct: 6 KRKNQQEHIPAEDLETPKLPKREKIEGTKESNKVRIIILLGYSGYGYHGIQINN---PLK 62
Query: 123 TVEGTILETMNKCKLVPS--MVPQEYKFSRCGRTDRGVSAMNQVISLRVRSNLTAEEQLD 180
T+EG ++ + K + + + + +R RTD+GV + +ISL NL ++ LD
Sbjct: 63 TIEGDVVAVLKKLGYLKTNNIDAEHLCIARAARTDKGVHTLRNLISL----NLFVDKPLD 118
Query: 181 PSYDDKEIQYVNILNQILPEDIRVSAVCLRPPKGFDARFSCKSRHYKYLFSSEGL 235
S E LN+ L IRV +V PK F+ R SC+SR Y+YL S L
Sbjct: 119 ISLLKTE------LNEALCSQIRVWSV-FPAPKYFNPRISCESRTYEYLIPSFAL 166
>At2g30320 [J] KOG2553 Pseudouridylate synthase
Length = 510
Score = 65.1 bits (157), Expect = 2e-10
Identities = 53/183 (28%), Positives = 87/183 (46%), Gaps = 22/183 (12%)
Query: 90 FSKYNTRFVAFKFAYLGWNYNGLAIQKDATPLPTVEGTILETMNKC-KLVPSMVPQEYK- 147
+ Y + V + Y+G +Y GL IQ+D + T+EG + + K + S +K
Sbjct: 57 WESYRKKKVVIRIGYVGTDYRGLQIQRDDPSIKTIEGELEVAIYKAGGIRDSNYGDLHKI 116
Query: 148 -FSRCGRTDRGVSAMNQVISLRVRSNLTAEEQLDPSYDDKEIQYVNILNQILPEDIRVSA 206
++R RTD+GV ++ ISL++ TA + D + +++ LPE+IRV +
Sbjct: 117 GWARSSRTDKGVHSLATSISLKMEIPETAWKD-----DPQGTVLAKCISKHLPENIRVFS 171
Query: 207 VCLRPPKGFDARFSCKSRHYKYL-----------FSSEGLD--LERMQLAAKHYEGEHDF 253
V L + FD R C R Y YL F+S+ +D + K +EGE+ F
Sbjct: 172 V-LPSNRRFDPRRECTLRKYSYLLPVDVLGIKNSFTSDEIDYHITDFNEILKEFEGEYPF 230
Query: 254 RNF 256
N+
Sbjct: 231 HNY 233
>CE20158 [J] KOG2553 Pseudouridylate synthase
Length = 415
Score = 63.5 bits (153), Expect = 6e-10
Identities = 74/280 (26%), Positives = 113/280 (39%), Gaps = 51/280 (18%)
Query: 77 KNVKKPRKSREFDFSKYNTRFVAFKFAYLGWNYNGLAIQKDATPLPTVEGTILETMNKCK 136
K KKPR + +KY A Y G Y G+ +QKD LPT+E +LE M
Sbjct: 23 KEPKKPRVKQ----AKY-----AMLLGYQGKKYYGMQLQKD---LPTIESKLLEAMETAG 70
Query: 137 LVPSMV---PQEYKFSRCGRTDRGVSAMNQVISLRVRSNLTAEEQLDPSYDDKEIQYVNI 193
+ + P ++ F R RTDR VSA Q+ +++ + D Y + + I
Sbjct: 71 WITTAQKEKPFDFFFQRAARTDRAVSAARQMCGMQLPRD-------DAKY---QAEGAGI 120
Query: 194 LNQILPEDIRVSAVCLRPPKGFDARFSCKSRHYKYL----------------FSSEGLDL 237
LN ILP DIRV + R F + C R Y Y F L
Sbjct: 121 LNNILPADIRVFGM-RRTTNFFHPQKQCDHRTYSYTCPSFVFAKPTELTNSSFRLSPETL 179
Query: 238 ERMQLAAKHYEGEHDFRNFCKLDGSKQITNYKRTI----KSSNIMH---VSEDMYCFDLV 290
+ Y G H+F N+ +++ + I K + ED+ +V
Sbjct: 180 AEVNSILSIYLGTHNFFNYTAKRAYDDMSSNRYIISFECKEPFLFRDDFRKEDVEFVQIV 239
Query: 291 --GSAFLWHQVRCMVANLFLVGQGLEDPNLIKEMLDIEKM 328
G +F+ HQ+R MV + V + L + I+ + ++M
Sbjct: 240 IKGQSFVLHQIRKMVGMVITVIRELHLKSSIQRSFEGQRM 279
>At1g20370 [J] KOG2553 Pseudouridylate synthase
Length = 549
Score = 62.0 bits (149), Expect = 2e-09
Identities = 50/148 (33%), Positives = 67/148 (44%), Gaps = 21/148 (14%)
Query: 92 KYNTRFVAFKFAYLGWNYNGLAIQKDATPLPTVEGTILETMNKCKLVPSM---VPQEYKF 148
KY R VA FA+ G Y G+ A T+EG + E + VP P+ Y++
Sbjct: 54 KYRRRKVAIVFAFCGVGYQGMQKNPGAK---TIEGELEEALFHAGAVPDADRNKPRNYEW 110
Query: 149 SRCGRTDRGVSAMNQVISLRVRSNLTAEEQLDPSYDDKEIQYVNILNQILPEDIRVSAVC 208
+R RTD+GVSA+ QV+S R +DP +V LN LP+ IRV
Sbjct: 111 ARSARTDKGVSAVGQVVSGRF--------YVDPP------GFVERLNSKLPDQIRVFGY- 155
Query: 209 LRPPKGFDARFSCKSRHYKYLFSSEGLD 236
R F ++ C R Y YL LD
Sbjct: 156 KRVAPSFSSKKFCDRRRYVYLIPVFALD 183
>7300636 [J] KOG2553 Pseudouridylate synthase
Length = 410
Score = 61.6 bits (148), Expect = 2e-09
Identities = 67/258 (25%), Positives = 110/258 (41%), Gaps = 50/258 (19%)
Query: 99 AFKFAYLGWNYNGLAIQKDATPLPTVEGTILETMNKCKLVPSMVPQEYK---FSRCGRTD 155
A +Y G NY G+ + T+E + + M K K + ++ + F R RTD
Sbjct: 73 AILLSYCGANYYGMQRNPG---MQTIEEELFKAMLKHKWITEDSFEQIQISCFQRAARTD 129
Query: 156 RGVSAMNQVISLRVRSNLTAEEQLDPSYDDKEIQYVNILNQILPEDIRVSAVCLRPPKGF 215
+GVSA QV S+++ E+LD + N LP+ IR+ V R KGF
Sbjct: 130 KGVSAARQVCSVKL------PEELD----------LEAFNADLPQQIRLFGV-ERVTKGF 172
Query: 216 DARFSCKSRHYKYLFSS-------EGLD------------LERMQLAAKHYEGEHDFRNF 256
+A+ C +R Y Y + E +D L++++ K YEG +F NF
Sbjct: 173 NAKDQCNARTYTYTLPTVAFAPFEEKVDDVHDTFRISPELLQKVKETLKLYEGTKNFHNF 232
Query: 257 CK----LDGSKQITNYKRTIKSSNIMHVSEDM--YCFDLVGSAFLWHQVRCMVANLFLVG 310
LD S + + + SS +D+ + G +F+ HQ+R MV +
Sbjct: 233 TSKKSFLDPSSK--RFIMSFTSSEPFRSPQDIEFVTLKVKGQSFMLHQIRKMVGLAIAIV 290
Query: 311 QGLEDPNLIKEMLDIEKM 328
+G ++ L E++
Sbjct: 291 RGNTTAATLERALTEERL 308
>At1g76120 [J] KOG2553 Pseudouridylate synthase
Length = 463
Score = 59.3 bits (142), Expect = 1e-08
Identities = 48/142 (33%), Positives = 65/142 (44%), Gaps = 21/142 (14%)
Query: 98 VAFKFAYLGWNYNGLAIQKDATPLPTVEGTILETMNKCKLVPSMV---PQEYKFSRCGRT 154
VA FA+ G Y G+ A T+EG + E + VP + P+ Y F+R RT
Sbjct: 24 VAIIFAFCGVGYQGMQKNPGAK---TIEGELEEALFHAGAVPESIRGKPKLYDFARSART 80
Query: 155 DRGVSAMNQVISLRVRSNLTAEEQLDPSYDDKEIQYVNILNQILPEDIRVSAVCLRPPKG 214
D+GVSA+ QV+S R +DP + +VN LN LP IR+ P
Sbjct: 81 DKGVSAVGQVVSGRF--------IVDP------LGFVNRLNSNLPNQIRIFGYKHVTP-S 125
Query: 215 FDARFSCKSRHYKYLFSSEGLD 236
F ++ C R Y YL LD
Sbjct: 126 FSSKKFCDRRRYVYLLPVFALD 147
>YPL212c [J] KOG2553 Pseudouridylate synthase
Length = 544
Score = 56.2 bits (134), Expect = 9e-08
Identities = 54/189 (28%), Positives = 79/189 (41%), Gaps = 34/189 (17%)
Query: 63 KKRSADAAVEAERRKNV-----KKPRKSREFDFS--------KYNTRFVAFKFAYLGWNY 109
K R AD E +++K+ K+P+ D + + R VA Y G Y
Sbjct: 29 KARKADFDDEKDKKKDNDKHIDKRPKSGPRLDENGNPLPKEPRLPKRKVAVMVGYCGTGY 88
Query: 110 NGLAIQKDATPLPTVEGTILETMNKCKLVPSMVPQEYK---FSRCGRTDRGVSAMNQVIS 166
+G+ P PT+E + + + + + K F R RTD+GV A +IS
Sbjct: 89 HGMQYNP---PNPTIESALFKAFVEAGAISKDNSNDLKKNGFMRAARTDKGVHAGGNLIS 145
Query: 167 LRVRSNLTAEEQLDPSYDDKEIQYVNILNQILPEDIRVSAVCLRPPKGFDARFSCKSRHY 226
L++ DP K +N+ LPE IRV + R K FD R C SR Y
Sbjct: 146 LKMIIE-------DPDIKQK-------INEKLPEGIRVWDI-ERVNKAFDCRKMCSSRWY 190
Query: 227 KYLFSSEGL 235
+YL + L
Sbjct: 191 EYLLPTYSL 199
>SPCC126.03 [J] KOG2553 Pseudouridylate synthase
Length = 534
Score = 52.8 bits (125), Expect = 1e-06
Identities = 44/137 (32%), Positives = 65/137 (47%), Gaps = 21/137 (15%)
Query: 96 RFVAFKFAYLGWNYNGLAIQKDATPLPTVEGTILETMNKCKLVPSMV---PQEYKFSRCG 152
R VA Y G Y+G+ + P T+EG + + K V S P++ +R
Sbjct: 44 RKVACLVGYCGSGYHGMQLNP---PSKTIEGDLFDAFVKAGAVSSYNADDPKKVALARAA 100
Query: 153 RTDRGVSAMNQVISLRVRSNLTAEEQLDPSYDDKEIQYVNILNQILPEDIRVSAVCLRPP 212
RTD+GV A VISL++ D+K I+ V N+ LP IR+ V +R
Sbjct: 101 RTDKGVHAAGNVISLKLIME-----------DEKLIEKV---NEHLPPSIRLWDV-IRTI 145
Query: 213 KGFDARFSCKSRHYKYL 229
F+ R C+SR Y+Y+
Sbjct: 146 NSFNPRTYCESRIYEYM 162
>Hs18545392 [AJ] KOG4393 Predicted pseudouridylate synthase
Length = 303
Score = 52.8 bits (125), Expect = 1e-06
Identities = 62/260 (23%), Positives = 101/260 (38%), Gaps = 55/260 (21%)
Query: 102 FAYLGWNYNGLAIQKDATPLPTVEGTILETMNKCKLVPSMVPQEYKFSRCGRTDRGVSAM 161
F Y+G ++NG+A + V+ + E + V + +F+ RTD GV A+
Sbjct: 18 FQYVGTDFNGVAAVRGTQRAVGVQNYLEEAAERLNSV-----EPVRFTISSRTDAGVHAL 72
Query: 162 NQVISLRVRSNLTAEEQLDPSYDDKEIQYVNILNQILPEDIRVSAV----CLRPPKGFDA 217
+ L V+ + P +L + L +R A+ R P F A
Sbjct: 73 SNAAHLDVQRR-SGRPPFPPE----------VLAEALNTHLRHPAIRVLRAFRVPSDFHA 121
Query: 218 RFSCKSRHYKYLFSS---------------------EGLDLERMQLAAKHYEGEHDFRNF 256
R + SR Y Y ++ + LD+ MQ AA+H G HDF F
Sbjct: 122 RHAATSRTYLYRLATGCHRRDELPVFERNLCWTLPADCLDMVAMQEAAQHLLGTHDFSAF 181
Query: 257 CKLDGSKQITNYKRTIK--------SSNIMHVSED----MYCFDLVGSAFLWHQVRCMVA 304
+ + RT++ +S ++ E + + +FL+ QVR M A
Sbjct: 182 --QSAGSPVPSPVRTLRRVSVSPGQASPLVTPEESRKLRFWNLEFESQSFLYRQVRRMTA 239
Query: 305 NLFLVGQGLEDPNLIKEMLD 324
L VG G P +K +L+
Sbjct: 240 VLVAVGLGALAPAQVKTILE 259
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.133 0.383
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 25,263,988
Number of Sequences: 60738
Number of extensions: 1058948
Number of successful extensions: 2936
Number of sequences better than 1.0e-05: 18
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 12
Number of HSP's that attempted gapping in prelim test: 2881
Number of HSP's gapped (non-prelim): 25
length of query: 444
length of database: 30,389,216
effective HSP length: 110
effective length of query: 334
effective length of database: 23,708,036
effective search space: 7918484024
effective search space used: 7918484024
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)