ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV3662 good O KOG2016 Posttranslational modification, protein turnover, chaperones NEDD8-activating complex, APP-BP1/UBA5 component
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV3662 1272154 1270721 -478
(478 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YPL003w [O] KOG2016 NEDD8-activating complex APP-BP1/UBA5 component 147 3e-35
SPAC323.06c [O] KOG2016 NEDD8-activating complex APP-BP1/UBA5 co... 128 2e-29
At1g05180 [O] KOG2016 NEDD8-activating complex APP-BP1/UBA5 comp... 116 8e-26
Hs4502169 [O] KOG2016 NEDD8-activating complex APP-BP1/UBA5 comp... 110 5e-24
At2g32410 [O] KOG2016 NEDD8-activating complex APP-BP1/UBA5 comp... 110 5e-24
7294767 [O] KOG2016 NEDD8-activating complex APP-BP1/UBA5 component 95 2e-19
CE01166 [O] KOG2016 NEDD8-activating complex APP-BP1/UBA5 component 69 2e-11
>YPL003w [O] KOG2016 NEDD8-activating complex APP-BP1/UBA5 component
Length = 462
Score = 147 bits (372), Expect = 3e-35
Identities = 129/472 (27%), Positives = 224/472 (47%), Gaps = 50/472 (10%)
Query: 42 DRYDRQLMIWGHSGQDLLSQSHVCVVDDNQTLLMRECVKSLALMGIHEIT---VVFGSSN 98
+RYDRQL +WG GQD L++S VCVV T L++E K+L L GI +T V +
Sbjct: 2 ERYDRQLRLWGALGQDSLNRSRVCVVGP-ATPLLQEVFKNLVLAGISSLTWLKVECAVQS 60
Query: 99 GSDFVNKSNSDILGFDALESMNPDVTFHIWNENAVPIDSQHKLWNSFSIVICLCTAGPL- 157
GS F+ + D LE + + N+ + W FS+VI C
Sbjct: 61 GSLFLAELKKD------LEPLASKQLEYEENDLRKTLQQPQYDWTRFSVVILTCIGEQTA 114
Query: 158 ------LQRVMKLSAVPLIMASTNDNLGFVRLIGSEPHCVIDSHGHFT-FDLRLNRMWPE 210
++R P++ + G++ L+ SE H V+ +H +DLRL WPE
Sbjct: 115 MLDLNEIRRQRGTKFPPVLNTFVSGFYGYIYLVLSETHFVLQAHPDSKKYDLRLQNPWPE 174
Query: 211 LAEYHESFDVKNMSQEEVSSLPYSVLLYN-VGKSLKSKNIPINRKSVRAEFIELHDSILS 269
L Y ++FD+ M S +PY+VLL + K + N N + + ++ D I
Sbjct: 175 LINYVDTFDLSKMDTATFSGIPYTVLLMKCIAKLERDGN---NGRITIDQMKKVLDQICL 231
Query: 270 GPLTNDL----NFVEAERSSFLLYDQSG---PFPHNLTNI-LNSFHAEPNSTINRWVYQF 321
PL ND+ N+VEA+R ++L Q+ L N+ ++ + + + T N +
Sbjct: 232 -PLGNDVIYEPNYVEAKRYAYLACSQNDCCKELEDLLRNLEISDYGNDWHDTYNYEILTL 290
Query: 322 VRCIRIFYDLRGDL---PVSAFIPDMESSTKLFNAQKAVYQRKAAKDKSEIMRILAKENY 378
+ ++ G+L P++ +PDMES+T+ + K +Y+ KA DKS + LA+
Sbjct: 291 LLTLKNIAKENGELSFQPLTGTLPDMESTTENYIRLKKLYEVKAKLDKSRVEESLARSK- 349
Query: 379 SLIPEPLIDKMLENLKHL--------DAIKMGSLSSATRTISEKPKFNKLLSLQNDRHDT 430
++ + +++ + + D + + S S+A +F + ++ + D
Sbjct: 350 KIVSQDVLETFCSHYGEVRKILPPKSDLLGIFSTSNALLDALVMVQFWEQPAVTAEDKD- 408
Query: 431 TFV------SPAMASILGAMVSQECVKLLTHQYVPVENTLLYDGDSNETSTF 476
F+ + ++ + G V QE +KL+TH YVP++N LY+G +N ++T+
Sbjct: 409 EFIGLRVDDNYSVMAFFGGAVVQEAIKLITHHYVPIDNLFLYNGINNSSATY 460
>SPAC323.06c [O] KOG2016 NEDD8-activating complex APP-BP1/UBA5 component
Length = 500
Score = 128 bits (322), Expect = 2e-29
Identities = 123/505 (24%), Positives = 215/505 (42%), Gaps = 82/505 (16%)
Query: 43 RYDRQLMIWGHSGQDLLSQSHVCVVDDNQTLLMRECVKSLALMGIHEITVVFGSSNGSDF 102
+YDRQ+ +W GQ+ + +SHVC++ N E +K+L L GI VV +S DF
Sbjct: 9 KYDRQVRLWKAEGQNAIEKSHVCLLYANTVGC--EALKNLILPGIGSFAVVDDTS--VDF 64
Query: 103 VNKSNSDILGFDA------------LESMNPDVTF-HIWNENAVPIDSQHKLWNSFSIVI 149
+ + +D L+ +NP+V ++ ID + ++ FS+V+
Sbjct: 65 SMDGMNFFIQYDQEGKSRARCTASLLQQLNPNVEMEYLEMSPEALIDKNIEYFSKFSVVL 124
Query: 150 CL-CTAGPL--LQRVMKLSAVPLIMASTNDNLGFVRLIGSEPHCVIDSHGHFTFDLRLNR 206
PL L+ ++ +PL+ ++ G +R I + + S DLRL
Sbjct: 125 SSNLKEKPLFRLEEYLRSHKIPLLHFNSVGFAGILR-ISTHEYTTTQSQPELPQDLRLKN 183
Query: 207 MWPELAEYHESFDVKNMSQEEVSSLPYSVLLYNVGKSLKSKNIPINRKSVRAEFIELHDS 266
WPEL Y +S D+ NM +S +PY VL+ +V + + ++++
Sbjct: 184 PWPELINYVKSMDLDNMDSSSLSEIPYIVLIIHVLLKVSPAHAQNSQEADDCAMFRKIME 243
Query: 267 ILSGPLTNDLNFVEAERSSFLLYDQSGPFPHNLTNILNSFHAEPNSTINRWVYQFVRCIR 326
G ++ N EA +S+ + + P N+ +L+ + + C++
Sbjct: 244 EYKGKCDSE-NIEEASSNSWKAFKEY-KLPSNVYEVLHDTRCVKIQEDSESFWIMAHCLK 301
Query: 327 IFYDLRGDLPVSAFIPDMESSTKLFNAQKAVYQRKAAKD----KSEIMRILAKENYSLIP 382
+FYD LP+S +PDM ST+ + + +Y+ K+ D K + + L + N S+
Sbjct: 302 MFYDETEFLPLSGLLPDMNCSTQQYVKLQVIYKEKSENDILKFKKYVQQTLKRLNRSV-- 359
Query: 383 EPLIDKMLE-------NLKHLDAIKM---------------------------GSLSSAT 408
E + D ++ N+K +D M + S T
Sbjct: 360 EEITDLEIKHFSRNCLNIKVMDFKTMKEEYQPTSNSAFRIYDTILEKHGKNYKEAFSDTT 419
Query: 409 RTISEKPKFNKLLSLQN---------------DRHDTTFVSPAMASILGAMVSQECVKLL 453
+TIS F + L+ D H+ +S S +G +V+QE +KLL
Sbjct: 420 KTISVAQSFLSQIGLEKFFDVVYTAIQELERADGHELHSIS----SFIGGIVAQETIKLL 475
Query: 454 THQYVPVENTLLYDGDSNETSTFIL 478
QY+P+ NT ++DG + T TF L
Sbjct: 476 AQQYLPLNNTFVFDGVHSRTETFKL 500
>At1g05180 [O] KOG2016 NEDD8-activating complex APP-BP1/UBA5 component
Length = 540
Score = 116 bits (290), Expect = 8e-26
Identities = 115/383 (30%), Positives = 185/383 (48%), Gaps = 39/383 (10%)
Query: 43 RYDRQLMIWGHSGQDLLSQSHVCVVDDNQTLLMRECVKSLALMGIHEITVVFGSSN---- 98
+YDRQL IWG GQ L ++ +C+++ T E +K+L L G+ ITVV GS
Sbjct: 23 KYDRQLRIWGEVGQAALEEASICLLNCGPT--GSEALKNLVLGGVGSITVVDGSKVQFGD 80
Query: 99 -GSDFVNKSNS-------DILGFDALESMNPDVTFHIWNENA-VPIDSQHKLWNSFSIVI 149
G++F+ + S + F L+ +N V EN I + ++ F++VI
Sbjct: 81 LGNNFMVDAKSVGQSKAKSVCAF--LQELNDSVNAKFIEENPDTLITTNPSFFSQFTLVI 138
Query: 150 C--LCTAGPL-LQRVMKLSAVPLIMASTNDNLGFVRLIGSEPHCVIDSH-GHFTFDLRLN 205
L L L R+ + + V L++ + GFVR+ E H +IDS HF DLRLN
Sbjct: 139 ATQLVEDSMLKLDRICRDANVKLVLVRSYGLAGFVRISVKE-HPIIDSKPDHFLDDLRLN 197
Query: 206 RMWPELAEYHESFDVKNMSQEEVS--SLPYSVLLYNVGK---SLKSKNIPINRKSVRAEF 260
WPEL + E+ D+ N+S+ + +PY V+L + + S N+P R+ + EF
Sbjct: 198 NPWPELKSFVETIDL-NVSEPAAAHKHIPYVVILVKMAEEWAQSHSGNLPSTREE-KKEF 255
Query: 261 IELHDSILSGPLTNDLNFVEAERSSFLLYDQSGPFPHNLTNILNSFHAEPNSTINR-WVY 319
+L S + T++ N+ EA ++F ++ G + ++N AE NS + WV
Sbjct: 256 KDLVKSKMVS--TDEDNYKEAIEAAFKVFAPRG-ISSEVQKLINDSCAEVNSNSSAFWVM 312
Query: 320 QFVRCIRIFYDLRGDLPVSAFIPDMESSTKLFNAQKAVYQRKAAKD----KSEIMRILAK 375
+ + G+ P+ IPDM SST+ + + +Y KA D + + IL K
Sbjct: 313 VAALKEFVLNEGGGEAPLEGSIPDMTSSTEHYINLQKIYLAKAEADFLVIEERVKNILKK 372
Query: 376 --ENYSLIPEPLIDKMLENLKHL 396
+ S IP+P I +N + L
Sbjct: 373 IGRDPSSIPKPTIKSFCKNARKL 395
>Hs4502169 [O] KOG2016 NEDD8-activating complex APP-BP1/UBA5 component
Length = 534
Score = 110 bits (275), Expect = 5e-24
Identities = 107/384 (27%), Positives = 187/384 (47%), Gaps = 41/384 (10%)
Query: 41 DDRYDRQLMIWGHSGQDLLSQSHVCVVDDNQTLLMRECVKSLALMGIHEITVVFGSS-NG 99
+ +YDRQL +WG GQ+ L +HVC++ N T E +K+L L GI T++ G+ +G
Sbjct: 10 EQKYDRQLRLWGDHGQEALESAHVCLI--NATATGTEILKNLVLPGIGSFTIIDGNQVSG 67
Query: 100 SD-----FVNKS----NSDILGFDALESMNPDVTFHIWNENAVPI-DSQHKLWNSFSIVI 149
D F+ +S N + L+ +N DV+ E+ + D+ + F++V+
Sbjct: 68 EDAGNNFFLQRSSIGKNRAEAAMEFLQELNSDVSGSFVEESPENLLDNDPSFFCRFTVVV 127
Query: 150 CLC---TAGPLLQRVMKLSAVPLIMASTNDNLGFVRLIGSEPHCVIDSHGHFTF-DLRLN 205
+ L V+ S +PL++ T +G++R+I E H VI+SH DLRL+
Sbjct: 128 ATQLPESTSLRLADVLWNSQIPLLICRTYGLVGYMRIIIKE-HPVIESHPDNALEDLRLD 186
Query: 206 RMWPELAEYHESFDVKNMSQEEVSSLPYSVLLYNVGKSLKSK---NIPINRKSVRAEFIE 262
+ +PEL E+ +S+D+ +M +++ S P+ V++ S+ IP K E +
Sbjct: 187 KPFPELREHFQSYDLDHMEKKDHSHTPWIVIIAKYLAQWYSETNGRIPKTYK----EKED 242
Query: 263 LHDSILSGPLTN-------DLNFVEAERSSFLLYDQSGPFPHNLTNILNSFHA--EPNST 313
D I G L N + NF EA ++ + + P ++ +I N T
Sbjct: 243 FRDLIRQGILKNENGAPEDEENFEEAIKNVNTALNTT-QIPSSIEDIFNDDRCINITKQT 301
Query: 314 INRWVYQFVRCIRIFY--DLRGDLPVSAFIPDMESSTKLFNAQKAVYQRKAAKDKSEIMR 371
+ W+ R ++ F + +G+LPV IPDM + + + + VY+ KA KD + +
Sbjct: 302 PSFWI--LARALKEFVAKEGQGNLPVRGTIPDMIADSGKYIKLQNVYREKAKKDAAAVGN 359
Query: 372 ILAK--ENYSLIPEPLIDKMLENL 393
+AK ++ PE + +K L+ L
Sbjct: 360 HVAKLLQSIGQAPESISEKELKLL 383
>At2g32410 [O] KOG2016 NEDD8-activating complex APP-BP1/UBA5 component
Length = 523
Score = 110 bits (275), Expect = 5e-24
Identities = 113/410 (27%), Positives = 188/410 (45%), Gaps = 37/410 (9%)
Query: 43 RYDRQLMIWGHSGQDLLSQSHVCVVDDNQTLLMRECVKSLALMGIHEITVVFGSSN---- 98
+YDRQL IWG GQ L + +C+++ T E +K+L + GI IT+V GS
Sbjct: 7 KYDRQLRIWGELGQSALETASICLLNCGPT--GSEALKNLVIGGIGSITIVDGSKVEIGD 64
Query: 99 -GSDFVNKSNS-------DILGFDALESMNPDVTFHIWNENA-VPIDSQHKLWNSFSIVI 149
G++F+ + S + GF L+ +N V + EN I + ++ F++VI
Sbjct: 65 LGNNFMVDAKSVGQSRAKTVCGF--LQELNDSVKANFVEENPDTLISTDPSFFSQFTLVI 122
Query: 150 CLCTAGPL---LQRVMKLSAVPLIMASTNDNLGFVRLIGSEPHCVIDSHGHFTFDLRLNR 206
L R+ + + V L++A + GFVR+ E + H DLRLN
Sbjct: 123 ATQLVEDSMVKLDRICREANVMLVLARSYGLTGFVRISVKEHTAIETKPDHSLDDLRLNS 182
Query: 207 MWPELAEYHESFDVKNMSQEEVSSLPYSVLLYNVGKSL---KSKNIPINRKSVRAEFIEL 263
WPEL Y ES D+ +PY V+L V + S N+P S R E E
Sbjct: 183 PWPELKSYVESIDLNVEEPAAHKHIPYVVILVKVAEEWAQHHSGNLP----STREEKNEF 238
Query: 264 HDSILSGPLTND-LNFVEAERSSFLLYDQSGPFPHNLTNILNSFHAEPNS-TINRWVYQF 321
D + S ++ D N+ EA ++F ++ +G + +I + AE S + + WV
Sbjct: 239 KDLVKSKMVSADEENYKEALLAAFKVFAPTG-ISQEIQDINHDSCAEVGSNSSDFWVMVA 297
Query: 322 VRCIRIFYDLRGDLPVSAFIPDMESSTKLFNAQKAVYQRKAAKD----KSEIMRILAK-- 375
I + G++P+ +PDM SST+ + + +Y KA D + + IL K
Sbjct: 298 ALKEFISNEGGGEVPLEGSMPDMISSTEHYINLQKIYHSKAEADFLSMEQRVKSILVKVG 357
Query: 376 ENYSLIPEPLIDKMLENLKHLDAIKMGSLSSATRTISEKPKFNKLLSLQN 425
++ S I +P I +N + L + ++ ++ S + +K L+ +N
Sbjct: 358 QDPSSISKPTIKSFCKNARKLKVCRYRTIEDEFKSPS-TTELHKYLADEN 406
>7294767 [O] KOG2016 NEDD8-activating complex APP-BP1/UBA5 component
Length = 524
Score = 95.1 bits (235), Expect = 2e-19
Identities = 105/407 (25%), Positives = 191/407 (46%), Gaps = 39/407 (9%)
Query: 43 RYDRQLMIWGHSGQDLLSQSHVCVVDDNQTLLMRECVKSLALMGIHEITVVFGSS----- 97
+YDRQ+ +WG GQ LL + VC+V N T + E K L L GI TV GS+
Sbjct: 17 KYDRQIRLWGEHGQTLLEAATVCLV--NVTAVGCETAKGLVLPGIGGFTVADGSTVKEED 74
Query: 98 ------NGSDFVNKSNSDILGFDALESMNPDVTFHIWNENA-VPIDSQHKLWNSFSIVIC 150
S ++ KS + + L+ +NPDV +E+A + ++ ++SF +VI
Sbjct: 75 LGNNFFLDSSYLGKSKA-LACMQLLQELNPDVNGDYVDESADFLLANRPNFFDSFDLVIA 133
Query: 151 L----CTAGPLLQRVMKLSAVPLIMASTNDNLGFVRLIGSEPHCVIDSH-GHFTFDLRLN 205
T L +R+ +L+ VPLI + LG +RL E HC++++H + FDLRL
Sbjct: 134 SNLNEQTLLLLAERLWELN-VPLIYCRSLGMLGTIRLQIRE-HCIVEAHPDNRQFDLRLE 191
Query: 206 RMWPELAEYHESFDVKNMSQEEVSSLPYSVLLY---NVGKSLKSKNIPINRKSVRAEFIE 262
+ L E+ + +V S +P+ ++L+ NV + ++ R E +
Sbjct: 192 HPFDALREHLDGTEV-------TSKVPWLLVLHKYLNVWQKQQADGTQTPRN--YKEKNQ 242
Query: 263 LHDSILSGPLTNDLNFVEAERSSFLLYDQSGPFPHNLTNILNSFHAEPNSTINRWVYQFV 322
L ++I ++ N+ EA ++ + +G P +L I E + + +
Sbjct: 243 LKETIREEMKADEENYEEAIKAVNTAFG-AGQVPKSLKAIFEDDACEQLNKKSNVFWIMA 301
Query: 323 RCIR--IFYDLRGDLPVSAFIPDMESSTKLFNAQKAVYQRKAAKDKSEIMRILAKENYSL 380
+ ++ + ++ G LP+ +PDM ++T + A + +Y+++A +D ++ + L
Sbjct: 302 KALKHFVIHENEGHLPLPGVLPDMTANTDSYIALQHIYRQQALQDADQVYHKCQEYLKQL 361
Query: 381 -IPEPLIDKMLENLKHLDAIKMGSLSSATRTISEKPKFNKLLSLQND 426
+P ID+ L +A + ++ TR E K ++LL L D
Sbjct: 362 ALPADSIDERSVRLICKEAAGL-AVIRGTRIAEEYEKSSRLLPLVED 407
>CE01166 [O] KOG2016 NEDD8-activating complex APP-BP1/UBA5 component
Length = 525
Score = 68.9 bits (167), Expect = 2e-11
Identities = 82/352 (23%), Positives = 150/352 (42%), Gaps = 43/352 (12%)
Query: 43 RYDRQLMIWGHSGQDLLSQSHVCVVDDNQTLLMRECVKSLALMGIHEITVVFGSSNGSDF 102
RYDRQ+ +WG GQ + + CV+ + L E +KSL L G+ VV +
Sbjct: 9 RYDRQVRLWGEEGQASIGSTSACVLGSDS--LATEILKSLVLAGVQSFYVVDDAKVEQAD 66
Query: 103 VNKS---NSDILG-------FDALESMNPDVTFHIWNE--NAVPIDSQHKLWNSFSIVIC 150
+ ++ ++D +G + L +NP V+ ++ A+ ++ KL +FS+V+
Sbjct: 67 IGQNFFLHADDIGRSRAEATLEKLTELNPSVSGSASSQPPTALAMEDVEKL-TTFSVVVA 125
Query: 151 LCTAGPLLQRVMKLSAVPLIMASTNDNLGFVRLIGSEPHCVIDSHGHFTF-DLRLNRMWP 209
+ I +T +G +R+ E H + +SH DLRL+ +
Sbjct: 126 -------------ANQNEEIDTTTFGLIGTIRICIKE-HTIANSHEENPRPDLRLDAPFS 171
Query: 210 ELAEYHESFDVKNMSQEEVSSLPYSVLLYNVGKSL-KSKNIP-------INRKSVRAEFI 261
+L E ++ M+ E++ PY +L + + K +N P RK ++A +
Sbjct: 172 KLIEMINETNLDEMTLEQLRHTPYILLHFKALEVFRKQRNDPEAFPSTTAERKELQAILM 231
Query: 262 ELHDSILSGPLTNDLNFVEAERSSFLLYDQSGPFPHNLTNILNSFHAEPNSTINRWVYQF 321
S + NF EA+ + + Q ++ +IL+S ST R +
Sbjct: 232 SFRRSSEESGTKDSENFDEAKAAVIRAF-QRTTIGSSVKSILSSPQC---STSTRPFWLI 287
Query: 322 VRCIRIFYDLRGD-LPVSAFIPDMESSTKLFNAQKAVYQRKAAKDKSEIMRI 372
+R F + LP+ +PDM S + + ++ KA D E++R+
Sbjct: 288 CEALRRFVTENNNLLPLRGTLPDMTSDSSRYTRLATLFHEKALSDAQEVLRL 339
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.319 0.135 0.393
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 27,759,343
Number of Sequences: 60738
Number of extensions: 1141738
Number of successful extensions: 2890
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 2872
Number of HSP's gapped (non-prelim): 14
length of query: 478
length of database: 30,389,216
effective HSP length: 110
effective length of query: 368
effective length of database: 23,708,036
effective search space: 8724557248
effective search space used: 8724557248
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)