ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV3676 good R KOG0254 General function prediction only Predicted transporter (major facilitator superfamily)
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV3676 1279763 1278678 -362
(362 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YHL047c [R] KOG0254 Predicted transporter (major facilitator sup... 418 e-117
YHL040c [R] KOG0254 Predicted transporter (major facilitator sup... 313 2e-85
YCL073c [R] KOG0254 Predicted transporter (major facilitator sup... 259 6e-69
YKR106w [R] KOG0254 Predicted transporter (major facilitator sup... 257 2e-68
YEL065w [R] KOG0254 Predicted transporter (major facilitator sup... 214 1e-55
YOL158c [R] KOG0254 Predicted transporter (major facilitator sup... 164 2e-40
SPBC4F6.09 [R] KOG0254 Predicted transporter (major facilitator ... 114 3e-25
SPCC61.01c [R] KOG0254 Predicted transporter (major facilitator ... 64 3e-10
>YHL047c [R] KOG0254 Predicted transporter (major facilitator superfamily)
Length = 637
Score = 418 bits (1074), Expect = e-117
Identities = 199/362 (54%), Positives = 254/362 (69%), Gaps = 2/362 (0%)
Query: 2 ELKQEKTFYQTHGLIQTLVQLFWKLDIIGLLLLTASMGCILVPLTIAGGIENEWKTGKII 61
EL+ EK++YQTHGL+Q LVQLFWKLD++G+LL TA +GCILVPLT+AGG+ W+ KII
Sbjct: 260 ELQDEKSYYQTHGLVQMLVQLFWKLDVVGVLLFTAGVGCILVPLTLAGGVSTNWRNSKII 319
Query: 62 XXXXXXXXXXXXTIYWESQWAKAPFAPFKLLKDRGIWAPMWMMFLVCFVYVMAAGYLYTI 121
IYWES+ A PFAPFKLLKDRG+WAP+ +MF +CFVY MAAGYLYTI
Sbjct: 320 GPFVLGFVLVPGFIYWESRLALVPFAPFKLLKDRGVWAPLGIMFFICFVYQMAAGYLYTI 379
Query: 122 LLVGMNQTDLSATRIMNLYSFVAGIYSPVFGFMVARTRRLKLYMIFGACLYFVVMGLFFR 181
L+V ++++ SATRI+NLYSFV + +P G +V R+ RLK Y+IFG LYF+ MGLF+R
Sbjct: 380 LVVAVDESASSATRIINLYSFVTAVVAPFLGLIVTRSSRLKSYIIFGGSLYFITMGLFYR 439
Query: 182 YRXXXXXXXXXXXXXXXWGVASCMYDYP-VTTAQSVTSHEHMATVTALLMTIFXXXXXXX 240
YR WG++SC++DYP + + QSVTSHE+MATVTAL T+F
Sbjct: 440 YRSGQDADGGIIAGMVIWGLSSCLFDYPTIVSIQSVTSHENMATVTALNYTVFRIGGAVA 499
Query: 241 XXXXXXXWTNLLYPRLLKNLGGDADLAAVAYDSPLDFILDYEWGTPIRAAMVEAYEHVQR 300
WT LYP+LL + GDADLA AY SPL FIL WGTP+R+AMVEAY HVQ+
Sbjct: 500 AAISGAIWTQSLYPKLLHYM-GDADLATAAYGSPLTFILSNPWGTPVRSAMVEAYRHVQK 558
Query: 301 YEVLVGLVFCVPMLILTFCIRDPKLVNEFGQQLNEGECVETEGEDPISEWISSRIKKLRR 360
YEV+V LVF PM +LTFC+RDP+L +F Q+L + E V+T+ +DPI++WI+ R K
Sbjct: 559 YEVIVALVFSAPMFLLTFCVRDPRLTEDFAQKLPDREYVQTKEDDPINDWIAKRFAKALG 618
Query: 361 RH 362
H
Sbjct: 619 GH 620
>YHL040c [R] KOG0254 Predicted transporter (major facilitator superfamily)
Length = 627
Score = 313 bits (802), Expect = 2e-85
Identities = 151/350 (43%), Positives = 218/350 (62%), Gaps = 3/350 (0%)
Query: 3 LKQEKTFYQTHGLIQTLVQLFWKLDIIGLLLLTASMGCILVPLTIAGGIENEWKTGKIIX 62
LK +K++YQ HG I+ L QLFW LD++G+LL+ S+GCILVPLT+AGG++ W ++I
Sbjct: 266 LKGQKSYYQEHGFIKILKQLFWMLDVVGVLLMGCSLGCILVPLTLAGGVKTTWNDSRLIG 325
Query: 63 XXXXXXXXXXXTIYWESQWAKAPFAPFKLLKDRGIWAPMWMMFLVCFVYVMAAGYLYTIL 122
WE ++A+ P P++L+KDR +W+ M + FL+ F+Y MAA YLYT++
Sbjct: 326 PFVLGFVLIPILWIWEYRFARDPILPYRLVKDRAVWSSMGISFLIDFIYYMAADYLYTVM 385
Query: 123 LVGMNQTDLSATRIMNLYSFVAGIYSPVFGFMVARTRRLKLYMIFGACLYFVVMGLFFRY 182
+V +N++ SATRI L SFV+ + SP F +V R RLK +++FG L+ V MGL + +
Sbjct: 386 IVAVNESVKSATRIATLSSFVSTVASPFFALLVTRCTRLKPFIMFGCALWMVAMGLLYHF 445
Query: 183 RXXXXXXXXXXXXXXXWGVASCMYDYPVT-TAQSVTSHEHMATVTALLMTIFXXXXXXXX 241
R WGV + ++ YPVT + QS SHE+MATVTAL T++
Sbjct: 446 RGGSQSHSGIIGALCVWGVGTTLFTYPVTVSVQSAVSHENMATVTALNYTLYRIGSAVGS 505
Query: 242 XXXXXXWTNLLYPRLLKNLGGDADLAAVAYDSPLDFILDYEWGTPIRAAMVEAYEHVQRY 301
WT LY ++LK + GD LA AY+SP FI Y WGTP R A++ AY++VQR
Sbjct: 506 AVSGAIWTQTLYKQILKRM-GDVALATTAYESPYTFIETYTWGTPQRNALMNAYKYVQRL 564
Query: 302 EVLVGLVFCVPMLILTFCIRDPKLVNEFG-QQLNEGECVETEGEDPISEW 350
E +V LVFCVP++ + C+RDPKL + + + +GE V+T+ DPI +W
Sbjct: 565 ETIVALVFCVPLIAFSLCLRDPKLTDTVAVEYIEDGEYVDTKDNDPILDW 614
>YCL073c [R] KOG0254 Predicted transporter (major facilitator superfamily)
Length = 615
Score = 259 bits (661), Expect = 6e-69
Identities = 144/362 (39%), Positives = 201/362 (54%), Gaps = 7/362 (1%)
Query: 3 LKQEKTFYQTHGLIQTLVQLFWKLDIIGLLLLTASMGCILVPLTIAGGIENEWKTGKIIX 62
LK++ +T GL + LV LFWKLDI+G+LL+T S+GCILVPLT+A +W KII
Sbjct: 251 LKEQARKERTGGLFENLVFLFWKLDIVGILLITVSLGCILVPLTLANETSQKWHNSKIIA 310
Query: 63 XXXXXXXXXXXTIYWESQWAKAPFAPFKLLKDRGIWAPMWMMFLVCFVYVMAAGYLYTIL 122
+YWE+++AK+P PFKLL DRGIWAP+ + F F + ++ YLY +L
Sbjct: 311 TLVSGGCLFFIFLYWEAKFAKSPLLPFKLLSDRGIWAPLGVTFFNFFTFFISCDYLYPVL 370
Query: 123 LVGMNQTDLSATRIMNLYSFVAGIYSPVFGFMVARTRRLKLYMIFGACLYFVVMGLFFRY 182
LV M ++ SA RI+NL FVA SP + +VA+TR+LKL +I G + V MGLF++Y
Sbjct: 371 LVSMKESSTSAARIVNLPDFVAATASPFYSLLVAKTRKLKLSVIGGCAAWMVCMGLFYKY 430
Query: 183 RXXXXXXXXXXXXXXXWGVASCMYDYPV-TTAQSVTSHEHMATVTALLMTIFXXXXXXXX 241
R G++ + V Q++T+H MA +T + T
Sbjct: 431 RGGSGSHEGVIAASVIMGLSGLLCSNSVIVILQAMTTHSRMAVITGIQYTFSKLGAAIGA 490
Query: 242 XXXXXXWTNLLYPRLLKNLGGDADLAAVAYDSPLDFILDYEWGTPIRAAMVEAYEHVQRY 301
WT + +L KNLG D LA +AY SP FI DY WG+P R A+VE+Y +VQR
Sbjct: 491 SVSGAIWTQTMPNQLYKNLGNDT-LAEIAYASPYTFISDYPWGSPERDAVVESYRYVQRI 549
Query: 302 EVLVGLVFCVPMLILTFCIRDPKLVNE-FGQQLNEGECVETEGEDPISEWISSRIKKLRR 360
+ VGL VP T +RDP+L+++ ++ E V P E I S+IK L R
Sbjct: 550 IMTVGLACTVPFFAFTMFMRDPELIDKATHEEFTEDGLVVL----PDEENIFSQIKALFR 605
Query: 361 RH 362
+
Sbjct: 606 HN 607
>YKR106w [R] KOG0254 Predicted transporter (major facilitator superfamily)
Length = 615
Score = 257 bits (656), Expect = 2e-68
Identities = 143/362 (39%), Positives = 201/362 (55%), Gaps = 7/362 (1%)
Query: 3 LKQEKTFYQTHGLIQTLVQLFWKLDIIGLLLLTASMGCILVPLTIAGGIENEWKTGKIIX 62
LK++ +T GL + LV LFWKLDI+G+LL+T S+GCILVPLT+A +W KII
Sbjct: 251 LKEQARKERTGGLFENLVFLFWKLDIVGILLITVSLGCILVPLTLANETSQKWHNSKIIA 310
Query: 63 XXXXXXXXXXXTIYWESQWAKAPFAPFKLLKDRGIWAPMWMMFLVCFVYVMAAGYLYTIL 122
+YWE+++AK+P PFKLL DRGIWAP+ + F F + ++ YLY +L
Sbjct: 311 TLVSGGCLFFIFLYWEAKFAKSPLLPFKLLSDRGIWAPLGVTFFNFFTFFISCDYLYPVL 370
Query: 123 LVGMNQTDLSATRIMNLYSFVAGIYSPVFGFMVARTRRLKLYMIFGACLYFVVMGLFFRY 182
LV M ++ SA RI+NL FVA SP + +VA+TR+LKL +I G + V MGLF++Y
Sbjct: 371 LVSMKESSTSAARIVNLPDFVAATASPFYSLLVAKTRKLKLSVIGGCAAWMVCMGLFYKY 430
Query: 183 RXXXXXXXXXXXXXXXWGVASCMYDYPV-TTAQSVTSHEHMATVTALLMTIFXXXXXXXX 241
R G++ + V Q++T+H MA +T + T
Sbjct: 431 RGGSGSHEGVIAASVIMGLSGLLCSNSVIVILQAMTTHSRMAVITGIQYTFSKLGAAIGA 490
Query: 242 XXXXXXWTNLLYPRLLKNLGGDADLAAVAYDSPLDFILDYEWGTPIRAAMVEAYEHVQRY 301
WT + +L KNLG D LA +AY SP FI DY WG+P R A+VE+Y +VQR
Sbjct: 491 SVSGAIWTQTMPNQLYKNLGNDT-LAEIAYASPYTFISDYPWGSPERDAVVESYRYVQRI 549
Query: 302 EVLVGLVFCVPMLILTFCIRDPKLVNE-FGQQLNEGECVETEGEDPISEWISSRIKKLRR 360
+ VGL VP T +R+P+L+++ ++ E V P E I S+IK L R
Sbjct: 550 IMTVGLACTVPFFTFTMFMRNPELIDKATHEEFTEDGLVVL----PDEENIFSQIKALFR 605
Query: 361 RH 362
+
Sbjct: 606 HN 607
>YEL065w [R] KOG0254 Predicted transporter (major facilitator superfamily)
Length = 628
Score = 214 bits (546), Expect = 1e-55
Identities = 115/316 (36%), Positives = 171/316 (53%), Gaps = 4/316 (1%)
Query: 23 FWKLDIIGLLLLTASMGCILVPLTIAGGIENEWKTGKIIXXXXXXXXXXXXT-IYWESQW 81
FWKLDIIG+LL+T GC+LVP T+AGG++ EWKT II + WE ++
Sbjct: 280 FWKLDIIGMLLITVFFGCVLVPFTLAGGLKEEWKTAHIIVPEVIGWVVVLPLYMLWEIKY 339
Query: 82 AKAPFAPFKLLKDRGIWAPMWMMFLVCFVYVMAAGYLYTILLVGMNQTDLSATRIMNLYS 141
++ P P+ L++DRGI+ + + F + F + M Y+YT+L+V ++++ SATRI +LYS
Sbjct: 340 SRHPLTPWDLIQDRGIFFALLIAFFINFNWYMQGDYMYTVLVVAVHESIKSATRITSLYS 399
Query: 142 FVAGIYSPVFGFMVARTRRLKLYMIFGACLYFVVMGLFFRYRXXXXXXXXXXXX--XXXW 199
FV+ I + GF++ + RR K ++IFG + V GL YR +
Sbjct: 400 FVSVIVGTILGFILIKVRRTKPFIIFGISCWIVSFGLLVHYRGDSGAHSGIIGSLCLLGF 459
Query: 200 GVASCMYDYPVTTAQSVTSHEHMATVTALLMTIFXXXXXXXXXXXXXXWTNLLYPRLLKN 259
G S Y + S +H MA VT+L + + WTN+L + K
Sbjct: 460 GAGSFTYVTQASIQASAKTHARMAVVTSLYLATYNIGSAFGSSVSGAVWTNILPKEISKR 519
Query: 260 LGGDADLAAVAYDSPLDFILDYEWGTPIRAAMVEAYEHVQRYEVLVGLVFCVPMLILTFC 319
+ D LAA AY SP FI Y WGTP R A+V +Y +VQ+ ++GLVFC P+L F
Sbjct: 520 I-SDPTLAAQAYGSPFTFITTYTWGTPERIALVMSYRYVQKILCIIGLVFCFPLLGCAFM 578
Query: 320 IRDPKLVNEFGQQLNE 335
+R+ KL + + N+
Sbjct: 579 LRNHKLTDSIALEGND 594
>YOL158c [R] KOG0254 Predicted transporter (major facilitator superfamily)
Length = 606
Score = 164 bits (415), Expect = 2e-40
Identities = 98/310 (31%), Positives = 156/310 (49%), Gaps = 7/310 (2%)
Query: 17 QTLVQLFWK----LDIIGLLLLTASMGCILVPLTIAGGIENEWKTGKIIXXXXXXXXXXX 72
QTL Q WK +++IG++L TA + +L+PLTIAGG ++W+ G II
Sbjct: 265 QTLRQTLWKFADDINLIGVILFTAFLVLVLLPLTIAGGATSKWREGHIIAMIVVGGCLGF 324
Query: 73 XTIYWESQWAKAPFAPFKLLKDRGIWAPMWMMFLVCFVYVMAAGYLYTILLVGMNQTDLS 132
+ WE ++AK PF P L D I+ + M F+ + YL T+L+V ++ LS
Sbjct: 325 IFLIWELKFAKNPFIPRVYLGDPTIYVALLMEFVWRLGLQIELEYLVTVLMVAFGESTLS 384
Query: 133 ATRIMNLYSFVAGIYSPVFGFMVARTRRLKLYMIFGACLYFVVMGLFFRYRXXXXXXXXX 192
A RI LY+F+ + V G M+ K++++ G+ L + MGL ++YR
Sbjct: 385 AQRIAQLYNFLQSCTNIVVGIMLHFYPHPKVFVVAGSLLGVIGMGLLYKYRVVYDGISGL 444
Query: 193 XXXXXXWGVASCMYDYPV-TTAQSVTSHEHMATVTALLMTIFXXXXXXXXXXXXXXWTNL 251
G+A M +P+ T + T+H MATVT LLM+++ WT
Sbjct: 445 IGAEIVVGIAGGMIRFPMWTLVHASTTHNEMATVTGLLMSVYQIGDAVGASIAGAIWTQR 504
Query: 252 LYPRLLKNLGGDADLAAVAYDSPLDFILDYEWGTPIRAAMVEAYEHVQRYEVLVGLVFCV 311
L L++ LG + L Y SPL+++ Y G+ +R M+E+Y +QR ++V + F
Sbjct: 505 LAKELIQRLG--SSLGMAIYKSPLNYLKKYPIGSEVRVQMIESYSKIQRLLIIVSISFAA 562
Query: 312 PMLILTFCIR 321
+L F +R
Sbjct: 563 FNAVLCFFLR 572
>SPBC4F6.09 [R] KOG0254 Predicted transporter (major facilitator superfamily)
Length = 612
Score = 114 bits (284), Expect = 3e-25
Identities = 85/307 (27%), Positives = 131/307 (41%), Gaps = 1/307 (0%)
Query: 20 VQLFWKLDIIGLLLLTASMGCILVPLTIAGGIENEWKTGKIIXXXXXXXXXXXXTIYWES 79
V+LF KLDIIGL+L+TA + +L+ +++A +W K I + +E
Sbjct: 291 VELFKKLDIIGLILMTAGLALVLLSISLASYDTGKWSDAKFIVMIIIGGLCLIAFVLYEI 350
Query: 80 QWAKAPFAPFKLLKDRGIWAPMWMMFLVCFVYVMAAGYLYTILLVGMNQTDLSATRIMNL 139
A P PF+L+++ I A M FL + Y Y+ L V + +A I
Sbjct: 351 FVASFPALPFRLMREPTIGACCAMSFLFYITFYCWDNYYYSFLQVVHYTSITAAGYISYT 410
Query: 140 YSFVAGIYSPVFGFMVARTRRLKLYMIFGACLYFVVMGLFFRYRXXXXXXXXXXXXXXXW 199
YSF + G ++ T+R K Y + +Y + GL RYR
Sbjct: 411 YSFTSCATGFFLGILIRLTKRYKWYFVASIPVYILGQGLMIRYRGEQYNWGYQIMPQIIV 470
Query: 200 GVASCMYDYPVTTA-QSVTSHEHMATVTALLMTIFXXXXXXXXXXXXXXWTNLLYPRLLK 258
G+ + +T A Q+V S E+ A VTAL+ T+ W +++ RL K
Sbjct: 471 GIGGGVIANLLTVAVQTVVSTENFAIVTALVSTVTPIGGAVGSAISGAIWNSVMPKRLEK 530
Query: 259 NLGGDADLAAVAYDSPLDFILDYEWGTPIRAAMVEAYEHVQRYEVLVGLVFCVPMLILTF 318
NL D A L L Y GT R A++ +Y VQ+ V F M+ +
Sbjct: 531 NLPSDLKDQAYTIFESLTVQLSYTRGTDARNAIILSYSEVQKILTSVATGFAGAMIFPVW 590
Query: 319 CIRDPKL 325
+ +P+L
Sbjct: 591 FVANPRL 597
>SPCC61.01c [R] KOG0254 Predicted transporter (major facilitator superfamily)
Length = 597
Score = 63.9 bits (154), Expect = 3e-10
Identities = 70/330 (21%), Positives = 127/330 (38%), Gaps = 9/330 (2%)
Query: 19 LVQLFWK-LDIIGLLLLTASMGCILVPLTIAGGIENEWKTGKIIXXXXXXXXXXXXTIYW 77
L LFW+ LD +G++L + +L+P + + + + T + ++
Sbjct: 273 LFYLFWQELDGLGIVLFVSGFTLLLLPFSHSSQVVSPDSTILTLFTITLSIALLVTLCFY 332
Query: 78 ESQWAKAPFAPFKLLKDRGIWAPMWMMFLVCFVYVMAAGYLYTILLVGMNQTDLSATRIM 137
+ ++A+ P K LKDR I ++F Y + + +L + L V + ++ +
Sbjct: 333 DVKYARYPVFALKSLKDRTILGSCVLIFTYFMSYYIFSNFLTSFLQVSYGLSIDMSSLTL 392
Query: 138 NLYSFVAGIYSPVFGFMVARTRRLKLYMIFGACLYFV-VMGLFFRYRXXXXXXXXXXXXX 196
N++ F + + GF++ R R K+ ++ +Y + ++G+
Sbjct: 393 NVFVFSMTTTAILSGFLMKRFGRFKMLLMISVPMYVLGILGIILFGINDNHYTRPLVLVL 452
Query: 197 XXWGVASCMYDYPVTTA-QSVTSHEHMATVTALLMTIFXXXXXXXXXXXXXXWTNLLYPR 255
G+ + A QSV+SH + L +T W+ L R
Sbjct: 453 ILAGMGGGLLTLSAQIAVQSVSSHAKLGMNLTLYLTFSSVGGAFGSAIAGGVWSKRLSSR 512
Query: 256 LLKNLGGDADLAAV-AYDSPLDFILDYEWGTPIRAAMVEAYEHVQRYEVLVGLVFCVPML 314
LL +L + + + L L Y GT IR + AY ++ + LV + M
Sbjct: 513 LLHDLKDHLPVPEIESIFRDLRTALSYPQGTQIRNIINVAYTATEKDLFHISLVASLFMF 572
Query: 315 ILTFCIRDPKLVNEFGQQLNEGECVETEGE 344
IRD L E N VET E
Sbjct: 573 AGLVIIRDVPLSTE-----NHDTAVETPSE 597
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.329 0.142 0.456
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,434,874
Number of Sequences: 60738
Number of extensions: 645228
Number of successful extensions: 1654
Number of sequences better than 1.0e-05: 8
Number of HSP's better than 0.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 1628
Number of HSP's gapped (non-prelim): 9
length of query: 362
length of database: 30,389,216
effective HSP length: 108
effective length of query: 254
effective length of database: 23,829,512
effective search space: 6052696048
effective search space used: 6052696048
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)