ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV3680 good R KOG0254 General function prediction only Predicted transporter (major facilitator superfamily)
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV3680 1280543 1279791 -251
(251 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YHL047c [R] KOG0254 Predicted transporter (major facilitator sup... 320 9e-88
YHL040c [R] KOG0254 Predicted transporter (major facilitator sup... 273 2e-73
YCL073c [R] KOG0254 Predicted transporter (major facilitator sup... 224 9e-59
YKR106w [R] KOG0254 Predicted transporter (major facilitator sup... 224 1e-58
YEL065w [R] KOG0254 Predicted transporter (major facilitator sup... 205 6e-53
SPBC4F6.09 [R] KOG0254 Predicted transporter (major facilitator ... 115 4e-26
SPAC1F8.03c [R] KOG0254 Predicted transporter (major facilitator... 102 5e-22
SPCC61.01c [R] KOG0254 Predicted transporter (major facilitator ... 97 3e-20
YOL158c [R] KOG0254 Predicted transporter (major facilitator sup... 96 5e-20
>YHL047c [R] KOG0254 Predicted transporter (major facilitator superfamily)
Length = 637
Score = 320 bits (821), Expect = 9e-88
Identities = 150/248 (60%), Positives = 192/248 (76%), Gaps = 5/248 (2%)
Query: 9 ITVKDENINKGFNNDEKEVAVDESLTPEKLNDFGNV-----DKLLATKSLVIRKAEIMAR 63
I V ++N + E +E + EK++D + DKL TKSL+IRK+E+MA+
Sbjct: 2 IEVPEDNRSSQTKRKNTEKNCNELMVDEKMDDDSSPRDEMKDKLKGTKSLIIRKSELMAK 61
Query: 64 QYDTWFYQTIFLLSAFICSYGYSLDSSIRDIYMTYAANDYDTHSLLTTIGIVNMMISAVA 123
+YDTW + IFL SAFIC++ Y LDSSIR YMTYA N Y HSL++T+ ++ +MISAV+
Sbjct: 62 KYDTWQLKAIFLFSAFICTFAYGLDSSIRGTYMTYAMNSYSAHSLISTVSVIVLMISAVS 121
Query: 124 QLFFAGLSDVFGRLSLMIVAIVFYVVGTVIQSQAYDIQKYCAGSVFYNVGLVGVMLQVTL 183
Q+ F GLSD+FGRL+L +V+IV Y+VGT+IQSQAYD+Q+Y AG+VFY VGLVGVMLQV L
Sbjct: 122 QVIFGGLSDIFGRLTLFLVSIVLYIVGTIIQSQAYDVQRYAAGAVFYYVGLVGVMLQVVL 181
Query: 184 FLSDNSSLRWRVLYNFVPAWPAIINMWVSGNIVEVANPLENWSWNIAMWAFIFPLSCIPL 243
LSDNSSL+WR+ Y +P+WP+II WVSG++VE ANPLENWSWNIAMWAFIFPL CIPL
Sbjct: 182 MLSDNSSLKWRLFYTLIPSWPSIITTWVSGSVVEAANPLENWSWNIAMWAFIFPLCCIPL 241
Query: 244 IVCVLHMR 251
I+C+LHMR
Sbjct: 242 ILCMLHMR 249
>YHL040c [R] KOG0254 Predicted transporter (major facilitator superfamily)
Length = 627
Score = 273 bits (698), Expect = 2e-73
Identities = 124/236 (52%), Positives = 169/236 (71%), Gaps = 11/236 (4%)
Query: 27 VAVDESLTPEKLNDFGN-----------VDKLLATKSLVIRKAEIMARQYDTWFYQTIFL 75
+ ++ L PE ND + + L +SL+I++ EI+ Y+ W+ Q I L
Sbjct: 19 MGMEHELNPETHNDSNSDSYGLPQLSEKYNALRQNRSLIIQQTEIIGSAYNKWYLQAILL 78
Query: 76 LSAFICSYGYSLDSSIRDIYMTYAANDYDTHSLLTTIGIVNMMISAVAQLFFAGLSDVFG 135
LSAFIC YGY LD +IR IY YA + Y HSLL+TI ++N ++SA +Q+ +A LSDVFG
Sbjct: 79 LSAFICGYGYGLDGNIRYIYTGYATSSYSEHSLLSTINVINAVVSAASQIIYARLSDVFG 138
Query: 136 RLSLMIVAIVFYVVGTVIQSQAYDIQKYCAGSVFYNVGLVGVMLQVTLFLSDNSSLRWRV 195
RL L I A++ YVVGT+IQSQAYD+Q+Y AG++FYN G VGV+L + + LSD SSL+WR+
Sbjct: 139 RLYLFISAVILYVVGTIIQSQAYDVQRYAAGAIFYNAGYVGVILILLIILSDFSSLKWRL 198
Query: 196 LYNFVPAWPAIINMWVSGNIVEVANPLENWSWNIAMWAFIFPLSCIPLIVCVLHMR 251
LY FVP WP IIN W++GNI ANP+ NWSW++ MWAFIFPLSC+P+++C+LHM+
Sbjct: 199 LYQFVPTWPFIINTWIAGNITSRANPVVNWSWDVGMWAFIFPLSCVPIVLCMLHMQ 254
>YCL073c [R] KOG0254 Predicted transporter (major facilitator superfamily)
Length = 615
Score = 224 bits (571), Expect = 9e-59
Identities = 100/197 (50%), Positives = 143/197 (71%)
Query: 55 IRKAEIMARQYDTWFYQTIFLLSAFICSYGYSLDSSIRDIYMTYAANDYDTHSLLTTIGI 114
+++ EIM+ Q+D+ Y+ + + +AF+C +G SLD ++R Y YA N Y HSLL+T+ +
Sbjct: 43 LKENEIMSAQFDSLKYKILLISTAFVCGFGISLDYTLRSTYTGYATNSYSEHSLLSTVQV 102
Query: 115 VNMMISAVAQLFFAGLSDVFGRLSLMIVAIVFYVVGTVIQSQAYDIQKYCAGSVFYNVGL 174
+N ++S +Q+ ++ LSD FGRL L +VA +FY++GT+IQSQA + Y AGSVFYN G
Sbjct: 103 INAVVSVGSQVVYSRLSDHFGRLRLFLVATIFYIMGTIIQSQATRLTMYAAGSVFYNCGY 162
Query: 175 VGVMLQVTLFLSDNSSLRWRVLYNFVPAWPAIINMWVSGNIVEVANPLENWSWNIAMWAF 234
VG L +TL LSD SSL+WR+ Y + WP II W+SGNI+ ANP +NWSWNIAMWAF
Sbjct: 163 VGTNLLLTLILSDFSSLKWRMFYQYASYWPYIIIPWISGNIITAANPQKNWSWNIAMWAF 222
Query: 235 IFPLSCIPLIVCVLHMR 251
I+PLS +P+I +L+M+
Sbjct: 223 IYPLSALPIIFLILYMK 239
>YKR106w [R] KOG0254 Predicted transporter (major facilitator superfamily)
Length = 615
Score = 224 bits (570), Expect = 1e-58
Identities = 100/197 (50%), Positives = 143/197 (71%)
Query: 55 IRKAEIMARQYDTWFYQTIFLLSAFICSYGYSLDSSIRDIYMTYAANDYDTHSLLTTIGI 114
+++ EIM+ Q+D+ Y+ + + +AF+C +G SLD ++R Y YA N Y HSLL+T+ +
Sbjct: 43 LKENEIMSAQFDSLKYKILLISTAFVCGFGISLDYTLRSTYTGYATNSYSEHSLLSTVQV 102
Query: 115 VNMMISAVAQLFFAGLSDVFGRLSLMIVAIVFYVVGTVIQSQAYDIQKYCAGSVFYNVGL 174
+N ++S +Q+ ++ LSD FGRL L +VA +FY++GT+IQSQA + Y AGSVFYN G
Sbjct: 103 INAVVSVGSQVVYSRLSDHFGRLRLFLVATIFYIMGTIIQSQATRLTMYAAGSVFYNCGY 162
Query: 175 VGVMLQVTLFLSDNSSLRWRVLYNFVPAWPAIINMWVSGNIVEVANPLENWSWNIAMWAF 234
VG L +TL LSD SSL+WR+ Y + WP II W+SGNI+ ANP +NWSWNIAMWAF
Sbjct: 163 VGTNLLLTLILSDFSSLKWRMFYQYASYWPYIIIPWISGNIITAANPQKNWSWNIAMWAF 222
Query: 235 IFPLSCIPLIVCVLHMR 251
I+PLS +P+I +L+M+
Sbjct: 223 IYPLSTLPIIFLILYMK 239
>YEL065w [R] KOG0254 Predicted transporter (major facilitator superfamily)
Length = 628
Score = 205 bits (521), Expect = 6e-53
Identities = 93/197 (47%), Positives = 131/197 (66%), Gaps = 2/197 (1%)
Query: 55 IRKAEIMARQYDTWFYQTIFLLSAFICSYGYSLDSSIRDIYMTYAANDYDTHSLLTTIGI 114
+ EI A Y+ Y+ S F+ +Y Y LD +IR + YA + Y HSLL+T+
Sbjct: 54 VHNIEIYAEMYNRPIYRVALFFSLFLIAYAYGLDGNIRYTFQAYATSSYSQHSLLSTVNC 113
Query: 115 VNMMISAVAQLFFAGLSDVFGRLSLMIVAIVFYVVGTVIQSQAYDIQKYCAGSVFYNVGL 174
+ +I+AV Q+FFA LSD+FGR S+MIV+I+FY +GT+I+SQA +I ++ G FY +GL
Sbjct: 114 IKTVIAAVGQIFFARLSDIFGRFSIMIVSIIFYSMGTIIESQAVNITRFAVGGCFYQLGL 173
Query: 175 VGVMLQVTLFLSDNSSLRWRVLYNFVPAWPAIINMWVSGNIVEVANPLENWSWNIAMWAF 234
G++L + + SD S+L WR+L F+PA P IIN W+SGN+ + NW W I MWAF
Sbjct: 174 TGIILILEVIASDFSNLNWRLLALFIPALPFIINTWISGNVTSAID--ANWKWGIGMWAF 231
Query: 235 IFPLSCIPLIVCVLHMR 251
I PL+CIPL +C+LHMR
Sbjct: 232 ILPLACIPLGICMLHMR 248
>SPBC4F6.09 [R] KOG0254 Predicted transporter (major facilitator superfamily)
Length = 612
Score = 115 bits (289), Expect = 4e-26
Identities = 69/243 (28%), Positives = 120/243 (48%), Gaps = 6/243 (2%)
Query: 2 SVAEESSITVKDENINKGFNNDEKEVAVDESLTPEKLNDFGNVDKLLATKSLVIRKAEIM 61
++ +E+SI K+ ++ +DEK V+E + ++ K S +++ +
Sbjct: 30 NIQDETSI--KEVSLQDAKQSDEK--GVEEKVFNDESFSIDVAPKPEDELSGIVKARYLT 85
Query: 62 ARQYDTWFYQTIFLLSAFICSYGYSLDSSIRDIYMTYAANDYDTHSLLTTIGIVNMMISA 121
FY F S F+ + S + YA + + HSLL+TI + N +ISA
Sbjct: 86 EHSSRISFYICYF--SIFLLFFAISFQAECYYSLTAYATSAFAGHSLLSTIAVANNIISA 143
Query: 122 VAQLFFAGLSDVFGRLSLMIVAIVFYVVGTVIQSQAYDIQKYCAGSVFYNVGLVGVMLQV 181
+ A LSDVFGRL + +++ Y+VG ++ + + ++Q Y GSV YN G GV L +
Sbjct: 144 AIKPPLARLSDVFGRLEAFLFSLLLYLVGLILMAASTNVQTYAGGSVLYNAGYTGVELIM 203
Query: 182 TLFLSDNSSLRWRVLYNFVPAWPAIINMWVSGNIVEVANPLENWSWNIAMWAFIFPLSCI 241
T+F++D SS+ R L + P ++ +W+ + + W W IA+W + P I
Sbjct: 204 TIFMADTSSMANRSLVLGISYLPFVVTIWIGPRVAQEFYMHSTWRWGIAVWTILIPACSI 263
Query: 242 PLI 244
P +
Sbjct: 264 PFL 266
>SPAC1F8.03c [R] KOG0254 Predicted transporter (major facilitator superfamily)
Length = 630
Score = 102 bits (254), Expect = 5e-22
Identities = 63/238 (26%), Positives = 111/238 (46%), Gaps = 14/238 (5%)
Query: 13 DENINKGFNNDEKEVAVDESLTPEKLNDFGNVDKLLATKSLVIRKAEIMARQYDTWFYQT 72
DE G+N +E +D SL V K+ A + + + M Y
Sbjct: 39 DEEKGSGYNTNETYSKMDNSLQHR------GVSKIEAVRDSIYQNKRGMYLAY------- 85
Query: 73 IFLLSAFICSYGYSLDSSIRDIYMTYAANDYDTHSLLTTIGIVNMMISAVAQLFFAGLSD 132
F ++ CS+ ++ SS Y YA ++ S+++T+ I +IS+V + SD
Sbjct: 86 AFGIAILACSWASAIQSSTTYSYQVYATASFNRTSMISTLEIATAIISSVCKPILGKFSD 145
Query: 133 VFGRLSLMIVAIVFYVVGTVIQSQAYDIQKYCAGSVFYNVGLVGVMLQVTLFLSDNSSLR 192
+ R + ++FYV+G ++ + + I Y GSVF ++G G+ TL + D +SL+
Sbjct: 146 ITSRPMTYTLVLLFYVIGFIVVASSSTISAYVIGSVFISIGSSGLDYLNTLVVGDLTSLK 205
Query: 193 WRVLYNFVPAWPAIINMWVSGNIVEVANPLENWSWNIAMWAFIFPLSCIPLIVCVLHM 250
WR + + P I +W +G IV+ NW W M+A I P P ++ ++++
Sbjct: 206 WRGFMTALLSTPYIATVWFTGFIVQGIID-SNWRWGYGMFAIIMPAVMTPAVIILMYL 262
>SPCC61.01c [R] KOG0254 Predicted transporter (major facilitator superfamily)
Length = 597
Score = 96.7 bits (239), Expect = 3e-20
Identities = 65/238 (27%), Positives = 121/238 (50%), Gaps = 13/238 (5%)
Query: 14 ENINKGFNNDEKEVAVDESLTP--EKLNDFGNVDKLLATKSLV-IRKAEIMARQYDTWFY 70
E+ K F N+E +++ P E+L++ G ++ + SL + KAE +A TW
Sbjct: 9 EHRKKSFRNNEN---LEQQFHPLREELDNAGPINDRTSELSLEGVSKAEAIA---STWSK 62
Query: 71 QTIFL--LSAFICSYGYSLDSSIRDIYMTYAANDYDTHSLLTTIGIVNMMISAVAQLFFA 128
++I + L ++ S+ SL+ YA +++ HS L TI +V ++ AV +
Sbjct: 63 RSIIVAYLGLYLLSFASSLEQQTTYSLQRYATSNFSAHSNLATINLVGNILLAVVRAPMV 122
Query: 129 GLSDVFGRLSLMIVAIVFYVVGTVIQSQAYDIQKYCAGSVFYNVGLVGVMLQVTLFLSDN 188
+DVFGR + +A+ V+G + + + +IQ + + Y G G+ L L ++D
Sbjct: 123 KAADVFGRSESLSLALGMTVLGYLSLAFSRNIQMFTVAYILYICGQTGLGLLSQLIIADT 182
Query: 189 SSLRWRVLYNFVPAWPAIINMWVSGNIVEVANPLENWSWNI--AMWAFIFPLSCIPLI 244
SSL R + + +P P + +W+ + + +P +N+ W + +WAFI P +PL+
Sbjct: 183 SSLLNRGILSAIPELPYLATVWIGPVLAQAFHPEKNYGWRLGYGIWAFILPTVSLPLL 240
>YOL158c [R] KOG0254 Predicted transporter (major facilitator superfamily)
Length = 606
Score = 95.9 bits (237), Expect = 5e-20
Identities = 59/178 (33%), Positives = 86/178 (48%), Gaps = 3/178 (1%)
Query: 71 QTIFLLSAFICSYGYSLDSSIRDIYMTYAANDYDTHSLLTTIGIVNMMISAVAQLFFAGL 130
+T L++ F+ Y L I TYAAN + HS + +I V ++++V + +A +
Sbjct: 63 KTALLVALFLQGYCTGLGGQISQSIQTYAANSFGKHSQVGSINTVKSIVASVVAVPYARI 122
Query: 131 SDVFGRLSLMIVAIVFYVVGTVIQSQAYDIQKYCAGSVFYNVGLVGVMLQVTLFLSDNSS 190
SD FGR+ I A+V Y +G +I + AG V G G L T D S
Sbjct: 123 SDRFGRIECWIFALVLYTIGEIISAATPTFSGLFAGIVIQQFGYSGFRLLATALTGDLSG 182
Query: 191 LRWRVLYNFVPAWPAIINMWVSGNIV-EVANPLE--NWSWNIAMWAFIFPLSCIPLIV 245
LR R + P IIN WVSGNIV VA + W W ++ I P+S + L++
Sbjct: 183 LRDRTFAMNIFLIPVIINTWVSGNIVSSVAGNVAPYKWRWGYGIFCIIVPISTLILVL 240
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.325 0.138 0.418
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,962,869
Number of Sequences: 60738
Number of extensions: 533853
Number of successful extensions: 1934
Number of sequences better than 1.0e-05: 9
Number of HSP's better than 0.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 1921
Number of HSP's gapped (non-prelim): 9
length of query: 251
length of database: 30,389,216
effective HSP length: 104
effective length of query: 147
effective length of database: 24,072,464
effective search space: 3538652208
effective search space used: 3538652208
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)