ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV3811 good S KOG2665 Function unknown Predicted FAD-dependent oxidoreductase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV3811 1322461 1323660 400
(400 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
At3g56840 [S] KOG2665 Predicted FAD-dependent oxidoreductase 196 4e-50
7298510 [S] KOG2665 Predicted FAD-dependent oxidoreductase 108 1e-23
Hs13376331 [S] KOG2665 Predicted FAD-dependent oxidoreductase 97 4e-20
CE28366 [S] KOG2665 Predicted FAD-dependent oxidoreductase 96 1e-19
>At3g56840 [S] KOG2665 Predicted FAD-dependent oxidoreductase
Length = 483
Score = 196 bits (499), Expect = 4e-50
Identities = 138/405 (34%), Positives = 211/405 (52%), Gaps = 52/405 (12%)
Query: 31 VIGAGVVGLAIANELTKVPGNKVIVLEKNAKIGQETSSRNSEVIHAGLYYPVDSLKTKFC 90
VIGAGVVGLA+A EL+ + G +V++L+ + G TSSRNSEV+HAG+YYP +SLK KFC
Sbjct: 84 VIGAGVVGLAVARELS-LRGREVLILDAASSFGTVTSSRNSEVVHAGIYYPPNSLKAKFC 142
Query: 91 IEGNHIIYNELNPRKTGVDWLKCGKWVVAQTDFEDAYVERMYYKAKYELNLPVEILPSHK 150
+ G ++Y + + + K GK +VA E ++ + + + +L +
Sbjct: 143 VRGRELLYKYCSEYE--IPHKKIGKLIVATGSSEIPKLDLLMHLGTQNRVSGLRMLEGFE 200
Query: 151 TQWQEPAVIVERSALVSPTTGIIDSHSLIEYLSAMVDNQGGDTVIGSEVKDIQFLG---S 207
EP + + AL+SP +GI+D+HS ++ ++V+ + D ++ + +++ G +
Sbjct: 201 AMRMEPQLRCVK-ALLSPESGILDTHS---FMLSLVE-KSFDFMVYRDNNNLRLQGEAQN 255
Query: 208 SYALQCVET--LNGTAXXXXXXXXXXXX----------------------AAGLYADKIA 243
++A T LNG +AGL A +A
Sbjct: 256 NHATFSYNTVVLNGRVEEKKMHLYVADTRFSESRCEAEAQLELIPNLVVNSAGLGAQALA 315
Query: 244 NMILPPERQV--KQYYAKGNYFT-SKTQVPAVRRLIYPVPPRNGKSLGTHLTIDLNHQVR 300
+ + + +YA+G YFT S + P +L+YP+P G LG H+T+DLN V+
Sbjct: 316 KRLHGLDHRFVPSSHYARGCYFTLSGIKAPPFNKLVYPIPEEGG--LGVHVTVDLNGLVK 373
Query: 301 FGPDLEYVDSADDLIP---------NPLNIEDAAKTITRYFPHLQPGELEASYCGIRPKL 351
FGPD+E+++ DD NP E I +Y+P L+ G LE Y GIRPKL
Sbjct: 374 FGPDVEWIECTDDTSSFLNKFDYRVNPQRSEKFYPEIRKYYPDLKDGSLEPGYSGIRPKL 433
Query: 352 AAPGDTEFKDFYIKEEE--GFPGFVNLLGIESPGLTSSIPIGRYV 394
+ P + DF I+ EE G PG VNL GIESPGLTSS+ I ++
Sbjct: 434 SGPKQSP-ADFVIQGEETHGVPGLVNLFGIESPGLTSSLAIAEHI 477
>7298510 [S] KOG2665 Predicted FAD-dependent oxidoreductase
Length = 455
Score = 108 bits (271), Expect = 1e-23
Identities = 107/409 (26%), Positives = 167/409 (40%), Gaps = 47/409 (11%)
Query: 26 DYSHAVIGAGVVGLAIANELT-KVPGNKVIVLEKNAKIGQETSSRNSEVIHAGLYYPVDS 84
DY V+G G+VG A A E+ + P KV VLEK K+ + S NS VIHAG+YY +
Sbjct: 42 DYDLVVVGGGIVGAASAREIVLRHPSLKVAVLEKECKLAKHQSGHNSGVIHAGIYYKPGT 101
Query: 85 LKTKFCIEGNHIIYNELNPRKTGVDWLKCGKWVVAQTDFEDAYVERMYYKAKYELNLP-V 143
LK + C+EG H+ Y L+ +K + + K GK +VA TD ++ + + K N+P +
Sbjct: 102 LKARLCVEGMHLAYAYLDEKK--IPYKKTGKLIVA-TDEKEVKLLKDLEKRGIANNVPDL 158
Query: 144 EILPSHKTQWQEPAVIVERSALVSPTTGIIDSHSLIEYLSAMVDNQGGDTVIGSEVKDIQ 203
++ + Q EP AL SP TGI+D + E+ GGD + V +
Sbjct: 159 RMIEGSEIQEIEP-YCQGVMALHSPHTGIVDWGLVTEHYGQDFKQCGGDIYLDFNVS--K 215
Query: 204 FLGSSYALQCVETLNGTAXXXXXXXXXXXXAAGLYADKIANMILPPERQVKQYYAKGNY- 262
F + T++G GL +D +A P R + +G Y
Sbjct: 216 FTETKEGTDYPVTIHGAKPGQTVRTKNVLTCGGLQSDLLAEKTGCP-RDPRIVPFRGEYL 274
Query: 263 FTSKTQVPAVRRLIYPVPPRNGKSLGTHLTIDLNHQVRFGPDL----------------- 305
+K + V+ IYPVP LG H T ++ + GP+
Sbjct: 275 LLTKEKQHMVKGNIYPVPDPRFPFLGVHFTPRMDGSIWLGPNAVLALKREGYTWGDINLF 334
Query: 306 ---------EYVDSADDLIPNPLN-------IEDAAKTITRYFPHLQPGELEASYCGIRP 349
+V A I L+ I K + +Y P + +++ G+R
Sbjct: 335 ELFDALRYPGFVKMASKYIGFGLSEMSKSWFINLQIKALQKYIPDITEYDIQRGPAGVRA 394
Query: 350 KLAAPGDTEFKDFYIKEEEGFPGF----VNLLGIESPGLTSSIPIGRYV 394
+ DF +G ++ SPG TSS+ I + +
Sbjct: 395 QAMDLDGNLVDDFVFDRGQGSGALAKRVLHCRNAPSPGATSSLAIAKMI 443
>Hs13376331 [S] KOG2665 Predicted FAD-dependent oxidoreductase
Length = 463
Score = 97.1 bits (240), Expect = 4e-20
Identities = 81/300 (27%), Positives = 135/300 (45%), Gaps = 15/300 (5%)
Query: 14 RPLFSRFYSAPVD-YSHAVIGAGVVGLAIANELT-KVPGNKVIVLEKNAKIGQETSSRNS 71
RPL SA + ++G G+VGLA A L + P + VLEK + + NS
Sbjct: 35 RPLCGGSRSASTSSFDIVIVGGGIVGLASARALILRHPSLSIGVLEKEKDLAVHQTGHNS 94
Query: 72 EVIHAGLYYPVDSLKTKFCIEGNHIIYNELNPRKTGVDWLKCGKWVVAQTDFEDAYVERM 131
VIH+G+YY +SLK K C++G ++Y ++ G+ + +CGK +VA E ++ +
Sbjct: 95 GVIHSGIYYKPESLKAKLCVQGAALLYEYC--QQKGISYKQCGKLIVAVEQEEIPRLQAL 152
Query: 132 YYKAKYELNLPVEILPSHKTQWQEPAVIVERSALVSPTTGIIDSHSLIEYLSAMVDNQGG 191
Y K + ++ + +EP A+ P TGI+D + + GG
Sbjct: 153 YEKGLQNGVPGLRLIQQEDIKKKEP-YCRGLMAIDCPHTGIVDYRQVALSFAQDFQEAGG 211
Query: 192 DTVIGSEVKDIQFLGSSYA-----LQCVETLNGTAXXXXXXXXXXXXAAGLYADKIANMI 246
+ EVK I+ S + +Q + T AGLY+D+I+ +
Sbjct: 212 SVLTNFEVKGIEMAKESPSRSIDGMQYPIVIKNT-KGEEIRCQYVVTCAGLYSDRISELS 270
Query: 247 -LPPERQVKQYYAKGNYFTSKTQ-VPAVRRLIYPVPPRNGKSLGTHLTIDLNHQVRFGPD 304
P+ ++ + +G+Y K + V+ IYPVP LG H T ++ + GP+
Sbjct: 271 GCTPDPRIVPF--RGDYLLLKPEKCYLVKGNIYPVPDSRFPFLGVHFTPRMDGSIWLGPN 328
>CE28366 [S] KOG2665 Predicted FAD-dependent oxidoreductase
Length = 433
Score = 95.5 bits (236), Expect = 1e-19
Identities = 104/414 (25%), Positives = 163/414 (39%), Gaps = 62/414 (14%)
Query: 27 YSHAVIGAGVVGLAIANELT-KVPGNKVIVLEKNAKIGQETSSRNSEVIHAGLYYPVDSL 85
Y ++G G+VG A A +L + P KV ++EK ++ S NS VIHAG+YY SL
Sbjct: 26 YDLVIVGGGIVGCATARQLLIEKPQLKVALIEKEKELAVHQSGHNSGVIHAGIYYTPGSL 85
Query: 86 KTKFCIEGNHIIYNELNPRKTGVDWLKCGKWVVAQTDFEDAYVERMYYKAKYELNLPVEI 145
K K C+EG + Y + K V + K GK +VA E ++ ++ +A+ +E+
Sbjct: 86 KAKLCVEGLDLSYEFFDKEK--VPYKKTGKLIVAVEPEEVPRLDALFSRAQTNGCRDIEM 143
Query: 146 LPSHKTQWQEPAVIVERSALVSPTTGIIDSHSLIEYLSAMVDNQGGDTVIGSEVKDIQ-- 203
+ S K EP AL SP TGI+D + + + +GG ++ I
Sbjct: 144 IDSSKITELEPH-CRGLKALWSPHTGIVDWGYVTKRFGEDFEKRGGKIYTSYPLEKISDN 202
Query: 204 -------FLGSSYALQCVETLNGTAXXXXXXXXXXXXAAGLYADKIANMILPPERQVKQY 256
+ S AL ET N AGL +D++A + K
Sbjct: 203 HDPGYPIRVSSGPALAEFETKN------------LITCAGLQSDRVA-ALSGCSTDPKIV 249
Query: 257 YAKGNYFTSKTQV-PAVRRLIYPVPPRNGKSLGTHLTIDLNHQVRFGPDL---------- 305
+G Y K + V+ IYPVP LG H T +N + GP+
Sbjct: 250 PFRGEYLLLKPEKRHLVKTNIYPVPDPRFPFLGVHFTPRMNGDIWLGPNAVLAYKREGYS 309
Query: 306 EYVDSADDLIPN-----------------------PLNIEDAAKTITRYFPHLQPGELEA 342
+ S DL+ + + I K + R+ P L+ ++
Sbjct: 310 YFSISPSDLLESLSYSGMQKLVKKHFTFGIKELYRGVWIAAQVKQLQRFIPELKLSDVTR 369
Query: 343 SYCGIRPKLAAPGDTEFKDFYIKEEEG--FPGFVNLLGIESPGLTSSIPIGRYV 394
G+R + DF G P +++ SP TSS+ I + +
Sbjct: 370 GPAGVRAQAMDSAGNLVDDFVFDSGTGKLSPLLMHVRNAPSPAATSSLAIAKMI 423
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.318 0.138 0.407
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 25,178,767
Number of Sequences: 60738
Number of extensions: 1115904
Number of successful extensions: 2285
Number of sequences better than 1.0e-05: 4
Number of HSP's better than 0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 2266
Number of HSP's gapped (non-prelim): 6
length of query: 400
length of database: 30,389,216
effective HSP length: 109
effective length of query: 291
effective length of database: 23,768,774
effective search space: 6916713234
effective search space used: 6916713234
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)