ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactV3831    suspect: LH   UD    KOG2218  Cell cycle control, cell division, chromosome partitioning     ER to golgi transport protein/RAD50-interacting protein 1
r_klactV3831    suspect: LH   UD    KOG2218  Intracellular trafficking, secretion, and vesicular transport  ER to golgi transport protein/RAD50-interacting protein 1

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactV3831 1330381 1328375 -669 
         (669 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YGL145w [UD] KOG2218 ER to golgi transport protein/RAD50-interac...   326   8e-89

>YGL145w [UD] KOG2218 ER to golgi transport protein/RAD50-interacting
           protein 1
          Length = 701

 Score =  326 bits (835), Expect = 8e-89
 Identities = 211/715 (29%), Positives = 372/715 (51%), Gaps = 66/715 (9%)

Query: 2   LQSIQDLINIDSHIKEVSSKRDELAERLKQLKDEEDDFINQMDLIEKRLDDATAEISSIS 61
           +  I DL+NI+  IK+V ++R+ELA +L+ LK        ++ L E    D     +S+ 
Sbjct: 1   MNGIDDLLNINDRIKQVQNERNELASKLQNLKQSLASNDTEVALSEVIAQDIIEVGASVE 60

Query: 62  NIAHIEPLRLKYGRLQVLDQIENLFRQKEVFRHETEVIEEIEKVSTEQLQS----VDLEH 117
            +   E LR KYG LQ+L+++E +  Q+   +   + ++  E+   E  +       L+ 
Sbjct: 61  GL---EQLRAKYGDLQILNKLEKVAVQQTQMQAGVDKLDSFERQLDELAEQPPDQFTLDD 117

Query: 118 LSTLKQRIVSL--------GIESM---------EVITRFNS-LLEAIAKELMEKFKQDLL 159
           +  L  ++ S+         I+S          +V  ++N  +++ +A      F Q LL
Sbjct: 118 VKALHSKLTSVFATVPQINNIDSQYAAYNKLKSKVTGKYNDVIIQRLATNWSNTFDQKLL 177

Query: 160 NSKWDTDYFVS-SKKIIHDIRQQSSTLFNLQTLWLD--------------DSSDDNENRQ 204
            ++WDT  F S S  ++  +R+ S+ L+ L  L+L                 S++N+   
Sbjct: 178 EAQWDTQKFASTSVGLVKCLRENSTKLYQLSLLYLPLEEETQNGDSERPLSRSNNNQEPV 237

Query: 205 YWNFQGISHNFLIKFMYHFSNPKFEDSGQQKQSIELYFKFLDRYLETKLFRCIDIFCDEK 264
            WNF+ +++NF ++F YHF       +      IE YF+FL+ YL   L++CI+IF D+ 
Sbjct: 238 LWNFKSLANNFNVRFTYHFH------ATSSSSKIETYFQFLNDYLAENLYKCINIFHDDC 291

Query: 265 SGLTKEFVHEQFINHTLNPIRNKITQTLLNISENQTNENLRTMIVLISQIFINDNALIKK 324
           +GLTK  +HEQFIN+ L PIR+K+  TL          +L+T+IVLISQI   D  L+  
Sbjct: 292 NGLTKPVIHEQFINYVLQPIRDKVRSTLF-------QNDLKTLIVLISQILATDKNLLNS 344

Query: 325 HYYTGIGLVSLLPTKVLEAWLEFELDSTLDQYEKIVSSA--LLTNGADLVKLLESMYQYF 382
            +Y G+GLVSL+  +V E W+ +E++    Q+  I  +      +  + VKL+  +Y Y 
Sbjct: 345 FHYHGLGLVSLISDEVWEKWINYEVEMANRQFINITKNPEDFPKSSQNFVKLINKIYDYL 404

Query: 383 EPCFNIDYYHLNSYKLQIVSRIFLELVSRYNKFIISPDPALSLLPEEKQLEITFLKLHNI 442
           EP +++D+  L  YKL   S IF+ L S Y  +I++ D       +E++L  T  KL ++
Sbjct: 405 EPFYDLDFDLLVRYKLMTCSLIFMNLTSSYLDYILTVDSLNETRTKEQELYQTMAKLQHV 464

Query: 443 VLLEQCVSELRFKPAFIELTYIFNKLSNSTYPSIFTESFSALEETVVT-VRESIIHRWKK 501
             + + +  L     FI+LT I N   +  Y S+F    +  E+ + T ++ SI+HR +K
Sbjct: 465 NFVYRKIKSLSSNFIFIQLTDIVNSTESKKYNSLFQNVENDYEKAMSTDMQNSIVHRIQK 524

Query: 502 ILKASLSPYFRLSKWQEIS-------NPPAQCSTELIGAIQKSSKLKAEFALLKLPTNIS 554
           +LK +L  YF++S W  +         P +  S EL+ +I    +L  +   + +P  IS
Sbjct: 525 LLKETLRNYFKISTWSTLEMSVDENIGPSSVPSAELVNSINVLRRLINKLDSMDIPLAIS 584

Query: 555 ISIFHGLLTQWIFYLKDYVVKVNNFSENGLKQVMLDYGELKTVLQLDDVPQCVQEEEFEE 614
           + + + LL   + Y  + ++K+N F++NGL Q + D+  L ++L L       +     E
Sbjct: 585 LKVKNELLNVIVNYFTESILKLNKFNQNGLNQFLHDFKSLSSILSLPSHATNYKCMSLHE 644

Query: 615 FMKVLSLKYNGKSQVLPFLSKGYMSQSQDYLEVRDALGLKYISNSELDYALYKIL 669
            +K+L LKY+  +Q   FL+  Y+ ++ ++  +++A  +KY+ ++++  ALY+I+
Sbjct: 645 LVKILKLKYDPNNQ--QFLNPEYI-KTGNFTSLKEAYSIKYLKDTKIQDALYRII 696


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.319    0.136    0.381 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 37,809,196
Number of Sequences: 60738
Number of extensions: 1607929
Number of successful extensions: 6527
Number of sequences better than 1.0e-05: 1
Number of HSP's better than  0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 6516
Number of HSP's gapped (non-prelim): 1
length of query: 669
length of database: 30,389,216
effective HSP length: 113
effective length of query: 556
effective length of database: 23,525,822
effective search space: 13080357032
effective search space used: 13080357032
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)