ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV3921 good J KOG2915 Translation, ribosomal structure and biogenesis tRNA(1-methyladenosine) methyltransferase, subunit GCD14
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV3921 1355166 1356386 407
(407 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YJL125c [J] KOG2915 tRNA(1-methyladenosine) methyltransferase su... 539 e-153
SPAC9G1.12 [J] KOG2915 tRNA(1-methyladenosine) methyltransferase... 340 3e-93
Hs17453131 [J] KOG2915 tRNA(1-methyladenosine) methyltransferase... 189 5e-48
At5g14600 [J] KOG2915 tRNA(1-methyladenosine) methyltransferase ... 189 8e-48
CE18988 [J] KOG2915 tRNA(1-methyladenosine) methyltransferase su... 182 8e-46
7301439 [J] KOG2915 tRNA(1-methyladenosine) methyltransferase su... 181 2e-45
Hs13435383 [J] KOG2915 tRNA(1-methyladenosine) methyltransferase... 54 3e-07
>YJL125c [J] KOG2915 tRNA(1-methyladenosine) methyltransferase subunit GCD14
Length = 383
Score = 539 bits (1388), Expect = e-153
Identities = 255/375 (68%), Positives = 310/375 (82%), Gaps = 2/375 (0%)
Query: 4 FFEYKDTIKEGDLVLAWLSRDNLKPITVKAGEVFNTRYGAFSHSDMIGKPFGSQIAIRTK 63
F YKD IKEGDL L W+SRDN+KP+ + + EVFNTRYG+F H D+IGKP+GSQIAIRTK
Sbjct: 6 FSGYKDLIKEGDLTLIWVSRDNIKPVRMHSEEVFNTRYGSFPHKDIIGKPYGSQIAIRTK 65
Query: 64 GSNRFGFIHVLQPTPELWSISLPHRTQIVYTPDSSYIMQRMECNPRSRVIEAGTGSGSFS 123
GSN+F F+HVLQPTPELW++SLPHRTQIVYTPDSSYIMQR+ C+P SRVIEAGTGSGSFS
Sbjct: 66 GSNKFAFVHVLQPTPELWTLSLPHRTQIVYTPDSSYIMQRLNCSPHSRVIEAGTGSGSFS 125
Query: 124 HAFARSAGHLFSYEFHEVRYEQAKQEFKEHGLLEAGNTTITHRDVCKDGFEIKNGDTTSH 183
HAFARS GHLFS+EFH +RYEQA +EFKEHGL++ N TITHRDVC+ GF IK GDTTS+
Sbjct: 126 HAFARSVGHLFSFEFHHIRYEQALEEFKEHGLID-DNVTITHRDVCQGGFLIKKGDTTSY 184
Query: 184 EFRGPEETKVELNADCIFLDLPAPWDAIPNLTSVISKEKKVNLCCFSPCIEQVDKTLEAL 243
EF G ET LNA+ +FLDLPAPWDAIP+L SVIS ++KV LCCFSPCIEQVDKTL+ L
Sbjct: 185 EF-GNNETAASLNANVVFLDLPAPWDAIPHLDSVISVDEKVGLCCFSPCIEQVDKTLDVL 243
Query: 244 EEEGWQDVQTVEIQGRQYEARRQMVRRLDDAIERLRDVKRRKHEGVEKRQKNVEQTSNNG 303
E+ GW DV+ VEIQGRQYE+RRQMVR L+DA+ERLRD+KR K +GVE+R++ T ++
Sbjct: 244 EKYGWTDVEMVEIQGRQYESRRQMVRSLNDALERLRDIKRHKLQGVERRKRMFNNTIDSN 303
Query: 304 SSVEATESDKDLKHTEKTHFNPFGKGSRVKEGDSNFEWTQVSKVELEIKSHTSFLTFASK 363
++ + TEK FNPFGKGSR+KEGDSN++W +V+K+E EIKSHTS+LTFA K
Sbjct: 304 DEKVGKRNEDGVPLTEKAKFNPFGKGSRIKEGDSNYKWKEVTKMEAEIKSHTSYLTFAFK 363
Query: 364 IIDRTRDEEQTKAYL 378
+++R+RD+E+ L
Sbjct: 364 VVNRSRDDEKVNEIL 378
>SPAC9G1.12 [J] KOG2915 tRNA(1-methyladenosine) methyltransferase subunit GCD14
Length = 364
Score = 340 bits (871), Expect = 3e-93
Identities = 169/368 (45%), Positives = 243/368 (65%), Gaps = 32/368 (8%)
Query: 4 FFEYKDTIKEGDLVLAWLSRDNLKPITVKAGEVFNTRYGAFSHSDMIGKPFGSQIAIRTK 63
F +YK ++ GDL +AW+ R+ L P+ ++A + F+ +YGAF HS+MIGK +G QIA K
Sbjct: 3 FADYKQRVENGDLAVAWIGRNKLIPLHIEAEKTFHNQYGAFPHSEMIGKRYGEQIASTAK 62
Query: 64 GSNRFGFIHVLQPTPELWSISLPHRTQIVYTPDSSYIMQRMECNPRSRVIEAGTGSGSFS 123
GFI++LQPTPELW+++LPHRTQIVYTPD + I Q++ +RVIEAGTGS S S
Sbjct: 63 Q----GFIYLLQPTPELWTLALPHRTQIVYTPDIALIHQKLRITYGTRVIEAGTGSASMS 118
Query: 124 HAFARSAG---HLFSYEFHEVRYEQAKQEFKEHGLL--EAGNTTITHRDVCKDGFEIKNG 178
HA +R+ G LF++E+H RY+ A QEF+EH +L GNT +THRDVCKDGF
Sbjct: 119 HAISRTVGPLGRLFTFEYHATRYQTALQEFREHEMLIDVGGNTHLTHRDVCKDGF----- 173
Query: 179 DTTSHEFRGPEETKVELNADCIFLDLPAPWDAIPNLTSVISKEKKVNLCCFSPCIEQVDK 238
E+ D IFLDLPAPW+AIP+L++ ++ +K +CCFSPCIEQ+
Sbjct: 174 ------------LDTEVKVDAIFLDLPAPWEAIPHLSNHVNHDKSTRICCFSPCIEQIQH 221
Query: 239 TLEALEEEGWQDVQTVEIQGRQYEARRQMVRRLDDAIERLRDVKRRKHEGVEKRQKNVEQ 298
+ EAL E GW D++ +E+ +Q+ AR+ + +D+AI+RL++VKRR+ EG E+R+ EQ
Sbjct: 222 SAEALRELGWCDIEMIEVDYKQWAARKSRIVHIDEAIDRLKEVKRRRIEGFERRKMRREQ 281
Query: 299 TSNNGSSVEATESDKDLKHTE-----KTHFNPFGKGSRVKEGDSNFEWTQVSKVELEIKS 353
++ + VE ++D L + +T P R++EGD N+EWT V++V+ +KS
Sbjct: 282 NLSSDAKVEDQDNDSMLGENKSSVSTETALKPV-TNKRIREGDGNYEWTDVARVDSNLKS 340
Query: 354 HTSFLTFA 361
HTS+L FA
Sbjct: 341 HTSYLLFA 348
>Hs17453131 [J] KOG2915 tRNA(1-methyladenosine) methyltransferase subunit GCD14
Length = 289
Score = 189 bits (481), Expect = 5e-48
Identities = 105/265 (39%), Positives = 155/265 (57%), Gaps = 30/265 (11%)
Query: 4 FFEYKDTIKEGDLVLAWLSRDNLKPITVKAGEVFNTRYGAFSHS-DMIGKPFGSQIAIRT 62
F Y++ IKEGD + L + + V+ G TR+G HS D+IG+PFGS++
Sbjct: 3 FVAYEELIKEGDTAILSLGHGAMVAVRVQRGAQTQTRHGVLRHSVDLIGRPFGSKVTC-- 60
Query: 63 KGSNRFGFIHVLQPTPELWSISLPHRTQIVYTPDSSYIMQRMECNPRSRVIEAGTGSGSF 122
R G+++VL PTPELW+++LPHRTQI+Y+ D + I +E P S V E+GTGSGS
Sbjct: 61 ---GRGGWVYVLHPTPELWTLNLPHRTQILYSTDIALITMMLELRPGSVVCESGTGSGSV 117
Query: 123 SHAFARS---AGHLFSYEFHEVRYEQAKQEFKEHGLLEAGNTTITHRDVCKDGFEIKNGD 179
SHA R+ GHL + EFH+ R E+A++EF+EH + T+ +DVC+ GF +
Sbjct: 118 SHAIIRTIAPTGHLHTVEFHQQRAEKAREEFQEHRV--GRWVTVRTQDVCRSGFGV---- 171
Query: 180 TTSHEFRGPEETKVELNADCIFLDLPAPWDAIPNLTSVISKEKKVNLCCFSPCIEQVDKT 239
SH AD +FLD+P+PW+A+ + + K + C FSPCIEQV +T
Sbjct: 172 --SHV------------ADAVFLDIPSPWEAVGHAWDAL-KVEGGRFCSFSPCIEQVQRT 216
Query: 240 LEALEEEGWQDVQTVEIQGRQYEAR 264
+AL G+ ++ T+E+ + Y R
Sbjct: 217 CQALAARGFSELSTLEVLPQVYNVR 241
>At5g14600 [J] KOG2915 tRNA(1-methyladenosine) methyltransferase subunit GCD14
Length = 370
Score = 189 bits (479), Expect = 8e-48
Identities = 115/324 (35%), Positives = 180/324 (55%), Gaps = 37/324 (11%)
Query: 5 FEYKDTIKEGDLVLAWLSRDNLKPITVKAGEVFNTRYGAFSHSDMIGKPFGSQIAIRTKG 64
F +K I++GDLV+ + D +KP+ V V R+G + HSD IGKP G+++
Sbjct: 10 FSFKRCIEDGDLVIVYERHDVMKPVKVSKDGVLQNRFGVYKHSDWIGKPLGTKVF----- 64
Query: 65 SNRFGFIHVLQPTPELWSISLPHRTQIVYTPDSSYIMQRMECNPRSRVIEAGTGSGSFSH 124
SN+ F+++L PTPELW++ L HRTQI+Y D S+++ +E P V+E+GTGSGS S
Sbjct: 65 SNKGKFVYLLAPTPELWTLVLSHRTQILYIADISFVVMYLEVVPGCVVLESGTGSGSLST 124
Query: 125 AFARS---AGHLFSYEFHEVRYEQAKQEFKEHGLLEAGNTTITHRDVCKDGFEIKNGDTT 181
+ AR+ GH++S++FHE R A+++F++ G+ + T+ RD+ +GF
Sbjct: 125 SLARAVAPTGHVYSFDFHEQRAVSAREDFEKTGI--SSLVTVEVRDIQGEGF-------- 174
Query: 182 SHEFRGPEETKVELNADCIFLDLPAPWDAIPNLTSVISKEKKVNLCCFSPCIEQVDKTLE 241
PE K+ AD +FLDLP PW A+P+ ++ ++ LC FSPCIEQV +T E
Sbjct: 175 ------PE--KLSGLADSVFLDLPQPWLAVPSAAKMLKQDGV--LCSFSPCIEQVQRTCE 224
Query: 242 ALEEEGWQDVQTVEIQGRQYEARR-------QMVRRLDDAIERLRDVKRRKHEGVEKRQK 294
L + + +++T E+ R YE + + ++ E +R K RKH E
Sbjct: 225 VLRSD-FIEIRTFEVLLRTYEVKEVKMDTSSMVGESHEEDNEGMRPCK-RKHRSNENDTV 282
Query: 295 NVEQTSNNGSSVEATESDKDLKHT 318
+ + +SN S V A + HT
Sbjct: 283 SQDNSSNASSVVMARPCSEARGHT 306
>CE18988 [J] KOG2915 tRNA(1-methyladenosine) methyltransferase subunit GCD14
Length = 312
Score = 182 bits (462), Expect = 8e-46
Identities = 105/305 (34%), Positives = 166/305 (54%), Gaps = 36/305 (11%)
Query: 4 FFEYKDTIKEGDLVLAWLSRDNLKPITVKAGEVFNTRYGAFSHSDMIGKPFGSQIAIRTK 63
F Y+D I+EGD V+ +++ + P VK G+ +YGA H +IGK +G +++
Sbjct: 8 FMSYEDRIQEGDTVIVYVTYGQMVPTIVKRGQTLMMKYGALRHEFIIGKRWGQRLSATA- 66
Query: 64 GSNRFGFIHVLQPTPELWSISLPHRTQIVYTPDSSYIMQRMECNPRSRVIEAGTGSGSFS 123
G++++L+PT +LW+ +LP RTQI+YT D + I+ ++ P S + E+GTGSGS S
Sbjct: 67 -----GYVYILRPTSDLWTRALPRRTQILYTADCAQILSLLDAKPGSVICESGTGSGSLS 121
Query: 124 HAFARS---AGHLFSYEFHEVRYEQAKQEFKEHGLLEAGNTTITHRDVCKDGFEIKNGDT 180
HA A + GHL++++ E R + +EFK HGL TT R+VC GF ++N
Sbjct: 122 HAIALTVAPTGHLYTHDIDETRTHKVLEEFKVHGL--GSVTTAVVRNVCTSGFHVENA-- 177
Query: 181 TSHEFRGPEETKVELNADCIFLDLPAPWDAIPNLTSVISKEKKVNLCCFSPCIEQVDKTL 240
AD +FLD+PAPW+AIP I + + L FSPC+EQV +
Sbjct: 178 ----------------ADGVFLDVPAPWEAIPFAAKSIFRARGGRLVSFSPCLEQVQRAC 221
Query: 241 EALEEEGWQDVQTVEIQGRQYEARRQMVRRLDDAIERLRDVKRRKHEGVEKRQKNVEQTS 300
A+ G+ ++T+E+ Q + Q + L++ E L D + +R++NV+ T
Sbjct: 222 LAMAAAGFVSIETIELVPEQKKIVSQHRQTLEE-FEELGDAYPK------ERKRNVDGTI 274
Query: 301 NNGSS 305
SS
Sbjct: 275 VEQSS 279
>7301439 [J] KOG2915 tRNA(1-methyladenosine) methyltransferase subunit GCD14
Length = 317
Score = 181 bits (459), Expect = 2e-45
Identities = 103/266 (38%), Positives = 155/266 (57%), Gaps = 38/266 (14%)
Query: 4 FFEYKDTIKEGDLVLAWLSRDNLKPITV------KAGE----VFNTRYGAFSHSDMIGKP 53
F + K I++GD+V+ +LS ++ I K GE +F T YG+ ++IG
Sbjct: 3 FLKPKTHIEKGDVVILYLSVSSMHAIEAVPEIVNKKGETIPHIFQTNYGSLKVENIIGVE 62
Query: 54 FGSQIAIRTKGSNRFGFIHVLQPTPELWSISLPHRTQIVYTPDSSYIMQRMECNPRSRVI 113
+GS++ + G+ HVLQPTPELW+ +LPHRTQI+YTPD S I+ ++E P + V+
Sbjct: 63 YGSKVELSK------GWAHVLQPTPELWTQTLPHRTQIIYTPDISMIIHQLEVRPGAVVV 116
Query: 114 EAGTGSGSFSHAFARS---AGHLFSYEFHEVRYEQAKQEFKEHGLLEAGNTTITHRDVCK 170
E+GTGSGS SH F R+ GHL +++FHE R +QA+ EF+ HG+ A T+ HRDVC
Sbjct: 117 ESGTGSGSLSHYFLRALKPTGHLHTFDFHEARADQARDEFRRHGI--ADFVTVYHRDVCN 174
Query: 171 DGFEIKNGDTTSHEFRGPEETKVELNADCIFLDLPAPWDAIPNLTSVISKEKKVNLCCFS 230
GF +++ AD +FLDLPAP A+P+ + K C FS
Sbjct: 175 LGF----------------GAELDGKADAVFLDLPAPDLAVPHAFKAL-KLSGGRFCSFS 217
Query: 231 PCIEQVDKTLEALEEEGWQDVQTVEI 256
PCIEQ + ++ L + G+ ++ ++E+
Sbjct: 218 PCIEQSQRCIQELTKLGFNEIVSLEV 243
>Hs13435383 [J] KOG2915 tRNA(1-methyladenosine) methyltransferase subunit GCD14
Length = 477
Score = 54.3 bits (129), Expect = 3e-07
Identities = 47/181 (25%), Positives = 82/181 (44%), Gaps = 19/181 (10%)
Query: 36 VFNTRYGAFSHSDMIGKPFGSQIAIRTKGSNRFGFIHVLQ-PTPELWSISLPHRTQIVYT 94
+ N+ +GA ++GK F QI + FG ++L+ P E + + + T I +
Sbjct: 179 LLNSNWGAVPFGKIVGK-FPGQIL-----RSSFGKQYMLRRPALEDYVVLMKRGTAITFP 232
Query: 95 PDSSYIMQRMECNPRSRVIEAGTGSGSFSHAFAR---SAGHLFSYEFHEVRYEQAKQEFK 151
D + I+ M+ NP V+EAG+GSG S ++ S G + S+E + ++ AK+ +K
Sbjct: 233 KDINMILSMMDINPGDTVLEAGSGSGGMSLFLSKAVGSQGRVISFEVRKDHHDLAKKNYK 292
Query: 152 EHGLLEAGNTTITHRDVCKDGFEIKNGDTTSHEFRGPEETKVELNADCIFLDLPAPWDAI 211
+ ++H + D N D + G E L D + LD+ P +
Sbjct: 293 HW----RDSWKLSHVEEWPD-----NVDFIHKDISGATEDIKSLTFDAVALDMLNPHVTL 343
Query: 212 P 212
P
Sbjct: 344 P 344
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.315 0.132 0.381
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 25,093,134
Number of Sequences: 60738
Number of extensions: 1130374
Number of successful extensions: 4421
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 4388
Number of HSP's gapped (non-prelim): 8
length of query: 407
length of database: 30,389,216
effective HSP length: 109
effective length of query: 298
effective length of database: 23,768,774
effective search space: 7083094652
effective search space used: 7083094652
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)