ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV3965 normal P KOG1341 Inorganic ion transport and metabolism Na+/K+ transporter
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV3965 1365717 1368923 1069
(1069 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YJL129c [P] KOG1341 Na+/K+ transporter 537 e-152
YKR050w [P] KOG1341 Na+/K+ transporter 516 e-146
SPAC3F10.02c [P] KOG1341 Na+/K+ transporter 385 e-106
SPAC1F5.12 [P] KOG1341 Na+/K+ transporter 336 1e-91
At4g10310 [P] KOG1341 Na+/K+ transporter 86 2e-16
>YJL129c [P] KOG1341 Na+/K+ transporter
Length = 1235
Score = 537 bits (1384), Expect = e-152
Identities = 277/493 (56%), Positives = 343/493 (69%), Gaps = 51/493 (10%)
Query: 437 YYGLHSGGD----PRNRLNRVMSTNYLSYEPKIGRNSTFVGLSESQKVELGGVEYRSTKV 492
YYGLH D PR+ L++ YLS++P IGRNS F+GL+ +QK ELGGVEYR+ K+
Sbjct: 715 YYGLHFDTDYDLDPRHDLSKGSGKTYLSWQPTIGRNSNFLGLTRAQKDELGGVEYRAIKL 774
Query: 493 LCLLLSIYYLGFSILAMIIFAAWIQPMNNYKEIVRSQGVSPTWWGFWTGMSAFGNLGLTL 552
LC +L +YY+G+ I+A ++ WI +Y E+VR GVSPTWWGFWT MSAF +LGLTL
Sbjct: 775 LCTILVVYYVGWHIVAFVMLVPWIILKKHYSEVVRDDGVSPTWWGFWTAMSAFNDLGLTL 834
Query: 553 TPNSMISFHKSIYTLIWMMWFIVIGNTGFPIFLRFVIWILFKLSPDLSLRKDSLGFLLDH 612
TPNSM+SF+K++Y LI M+WFI+IGNTGFPI LR +IWI+FK+SPDLS ++SLGFLLDH
Sbjct: 835 TPNSMMSFNKAVYPLIVMIWFIIIGNTGFPILLRCIIWIMFKISPDLSQMRESLGFLLDH 894
Query: 613 PRRCFTMLFPKAATWWLFLILIGMNAXXXXXXXXXXXNTDVVKDLTAGFKVLNGLFQAVS 672
PRRCFT+LFPKAATWWL L L G+N + VVK L+ G++VL GLFQ+VS
Sbjct: 895 PRRCFTLLFPKAATWWLLLTLAGLNITDWILFIILDFGSTVVKSLSKGYRVLVGLFQSVS 954
Query: 673 TRTAGFAVLDLSQLHPXXXXXXXXXXXXXXXPLAISIRRTNVYEEQSLGVYGN--GE--- 727
TRTAGF+V+DLSQLHP PLAISIRRTNVYEEQSLG+YG+ GE
Sbjct: 955 TRTAGFSVVDLSQLHPSIQVSYMLMMYVSVLPLAISIRRTNVYEEQSLGLYGDMGGEPED 1014
Query: 728 -NQEDDGSEK-----------------------------------------NTKSFIGAH 745
+ EDDG+++ +TKSFIGAH
Sbjct: 1015 TDTEDDGNDEDDDEENESHEGQSSQRSSSNNNNNNNRKKKKKKKTENPNEISTKSFIGAH 1074
Query: 746 LRRQLSFDLWYMFLGLFIICICEGGKIQDPSKPSFTVFQCLFEVVSAYGTVGLSLGYPGS 805
LR+QLSFDLW++FLGLFIICICEG KI+D +P+F +F LFE+VSAYGTVGLSLGYP +
Sbjct: 1075 LRKQLSFDLWFLFLGLFIICICEGDKIKDVQEPNFNIFAILFEIVSAYGTVGLSLGYPDT 1134
Query: 806 NQSFSGQFTTISKLVIIAMLIRGRHRGLPYTLDRAIILPSAKLKENDLLHELTHSRRPTT 865
NQSFS QFTT+SKLVIIAMLIRG++RGLPY+LDRAIILPS +L+ D L + R+ T
Sbjct: 1135 NQSFSRQFTTLSKLVIIAMLIRGKNRGLPYSLDRAIILPSDRLEHIDHLEGMKLKRQART 1194
Query: 866 LGLNGSTSHDNRT 878
+ T H R+
Sbjct: 1195 NTEDPMTEHFKRS 1207
Score = 220 bits (561), Expect = 8e-57
Identities = 140/347 (40%), Positives = 180/347 (51%), Gaps = 82/347 (23%)
Query: 1 MIFRKVV-RNPTVQNIQI-YRKTLGHQIRDIIEKLTKKIDPFVKFIFPNXXXXXXXXXXX 58
M FR+ + R PT+ +++I Y+K+ GH+ RD I P K+IFP+
Sbjct: 1 MHFRRTMSRVPTLASLEIRYKKSFGHKFRDFIALCGHYFAPVKKYIFPSFIAVHYFYTIS 60
Query: 59 XXLLCSILIYPVKNFAYIDILFLATGAATQGGLNTVDLNELSLYQQIVLYITTALTTPIF 118
L+ SIL+YP+KN YID LFLA GA TQGGLNTVD+N LSLYQQIVLYI ++TPI
Sbjct: 61 LTLITSILLYPIKNTRYIDTLFLAAGAVTQGGLNTVDINNLSLYQQIVLYIVCCISTPIA 120
Query: 119 IHSGLAFVRFYWFERYFDGIRDWSKRDFEMRRTMTLRQRELTKNNTMENASRSTGQGPLN 178
+HS LAFVR YWFERYFDGIRD S+R+F+MRRT T+ +RELT +N + +
Sbjct: 121 VHSCLAFVRLYWFERYFDGIRDSSRRNFKMRRTKTILERELTARTMTKNRTGTQ------ 174
Query: 179 FLTRTFTHGDRDASNFQGRLFRGEVVKRD------------------------------- 207
++ +FQ +LF GE+V RD
Sbjct: 175 --RTSYPRKQAKTDDFQEKLFSGEMVNRDEQDSVHSDQNSHDISRDSSNNNTNHNGSSGS 232
Query: 208 ------EDNNDDS----------------------SVDTRPP-------EAHSDH----- 227
ED DD+ S++ +P E HS
Sbjct: 233 LDDFVKEDETDDNGEYQENNSYSTVGSSSNTVADESLNQKPKPSSLRFDEPHSKQRPARV 292
Query: 228 -TEKFIGRRKSRDINPADMYRSIAMLQNRHESNDNIKGPALVIKSPA 273
+EKF RR SRDI+PADMYRSI MLQ +HE+ +GP LVI SPA
Sbjct: 293 PSEKFAKRRGSRDISPADMYRSIMMLQGKHEATAEDEGPPLVIGSPA 339
>YKR050w [P] KOG1341 Na+/K+ transporter
Length = 889
Score = 516 bits (1330), Expect = e-146
Identities = 258/440 (58%), Positives = 317/440 (71%), Gaps = 26/440 (5%)
Query: 444 GDPRNRLNRVMSTNYLSYEPKIGRNSTFVGLSESQKVELGGVEYRSTKVLCLLLSIYYLG 503
GD + R MSTNYLS++P GRNS F+GL++ QK ELGGVEYR+ ++LC +L +YY+G
Sbjct: 413 GDIGHPFTRTMSTNYLSWQPTFGRNSVFIGLTKQQKEELGGVEYRALRLLCCILMVYYIG 472
Query: 504 FSILAMIIFAAWIQPMNNYKEIVRSQGVSPTWWGFWTGMSAFGNLGLTLTPNSMISFHKS 563
F+ILA + W ++Y EI+R GVSPTWWGF+T MSAF NLGL+LT +SM+SF +
Sbjct: 473 FNILAFVTIVPWACTRHHYSEIIRRNGVSPTWWGFFTAMSAFSNLGLSLTADSMVSFDTA 532
Query: 564 IYTLIWMMWFIVIGNTGFPIFLRFVIWILFKLSPDLSLRKDSLGFLLDHPRRCFTMLFPK 623
Y LI+MM+FI+IGNTGFPI LRF+IWI+FK S DLS K+SLGFLLDHPRRCFT+LFP
Sbjct: 533 PYPLIFMMFFIIIGNTGFPIMLRFIIWIMFKTSRDLSQFKESLGFLLDHPRRCFTLLFPS 592
Query: 624 AATWWLFLILIGMNAXXXXXXXXXXXNTDVVKDLTAGFKVLNGLFQAVSTRTAGFAVLDL 683
TWWLF L+ +NA N+ VV+ + G++ L GLFQ+V TRTAGF V+DL
Sbjct: 593 GPTWWLFTTLVVLNATDWILFIILDFNSAVVRQVAKGYRALMGLFQSVCTRTAGFNVVDL 652
Query: 684 SQLHPXXXXXXXXXXXXXXXPLAISIRRTNVYEEQSLGVYGNGENQED------------ 731
S+LHP PLAISIRRTNVYEEQSLG+Y +G++ E+
Sbjct: 653 SKLHPSIQVSYMLMMYVSVLPLAISIRRTNVYEEQSLGLYDSGQDDENITHEDDIKETDH 712
Query: 732 --DGSEKNT------------KSFIGAHLRRQLSFDLWYMFLGLFIICICEGGKIQDPSK 777
+ E++T KSF+GAHLRRQLSFDLWY+FLGLFIICICEG KI+D +K
Sbjct: 713 DGESEERDTVSTKSKPKKQSPKSFVGAHLRRQLSFDLWYLFLGLFIICICEGRKIEDVNK 772
Query: 778 PSFTVFQCLFEVVSAYGTVGLSLGYPGSNQSFSGQFTTISKLVIIAMLIRGRHRGLPYTL 837
P F VF LFEVVSAYGTVGLSLGYP +N S S QFT +SKLVIIAMLIRGR+RGLPYTL
Sbjct: 773 PDFNVFAILFEVVSAYGTVGLSLGYPNTNTSLSAQFTVLSKLVIIAMLIRGRNRGLPYTL 832
Query: 838 DRAIILPSAKLKENDLLHEL 857
DRAI+LPS KL++ D L ++
Sbjct: 833 DRAIMLPSDKLEQIDRLQDM 852
Score = 192 bits (487), Expect = 3e-48
Identities = 113/283 (39%), Positives = 160/283 (55%), Gaps = 51/283 (18%)
Query: 17 IYRKTLGHQIRDIIEKLTKKIDPFVKFIFPNXXXXXXXXXXXXXLLCSILIYPVKNFAYI 76
+++K+ GH++RD I K P K++FPN ++ SIL+YP KN A+I
Sbjct: 22 VHKKSWGHRLRDFISGFLKSCRPIAKYVFPNFIVVHYIYLITLSIIGSILLYPCKNTAFI 81
Query: 77 DILFLATGAATQGGLNTVDLNELSLYQQIVLYITTALTTPIFIHSGLAFVRFYWFERYFD 136
D+LFLA GA+TQGGL T N+ +LYQQIV+Y+ T L+TPI IH LAFVR YWFERYFD
Sbjct: 82 DVLFLAAGASTQGGLATKSTNDFNLYQQIVVYVITLLSTPILIHGFLAFVRLYWFERYFD 141
Query: 137 GIRDWSKRDFEMRRTMTLRQRELTKNNTMENASRSTGQGPLNFLTRTFTHGDRDASNFQG 196
IRD SK++F++RRTMTL+QREL + + NA+RS +F+
Sbjct: 142 NIRDISKQNFKLRRTMTLQQREL--SGSSGNAARS--------------------RSFKD 179
Query: 197 RLFRGEVVKRDE----------DNNDDSSVDTRPPEAHSDHTEK----------FIGRRK 236
LFRG+ V R++ D+ D S++ + P S E+ F +R+
Sbjct: 180 NLFRGKFVSREDPRQSASDVPMDSPDTSALSSISPLNVSSSKEESSDTQSSPPNFSSKRQ 239
Query: 237 SRDINPADMYRSIAMLQNRHE-SNDN--------IKGPALVIK 270
D++P D+Y+SI MLQ + E SN N GPA +++
Sbjct: 240 PSDVDPRDIYKSIMMLQKQQEKSNANSTDSFSSETNGPAFIVQ 282
>SPAC3F10.02c [P] KOG1341 Na+/K+ transporter
Length = 841
Score = 385 bits (989), Expect = e-106
Identities = 195/409 (47%), Positives = 264/409 (63%), Gaps = 11/409 (2%)
Query: 454 MSTNYLSYEPKIGRNSTFVGLSESQKVELGGVEYRSTKVLCLLLSIYYLGFSILAMIIFA 513
MS YLSY P + RNS FV LS+ Q+ ELGG+EYR+ K +C ++++Y++ F+I A + F
Sbjct: 427 MSLPYLSYNPTVDRNSAFVALSKEQRDELGGIEYRALKCVCSMVTLYFIIFNIAAFVTFI 486
Query: 514 AWIQPMNNYKEIVRSQGVSPTWWGFWTGMSAFGNLGLTLTPNSMISFHKSIYTLIWMMWF 573
+ +E++ S + WW ++ S+F +LG +L P+S + +++I+ L+ F
Sbjct: 487 VFAYTAVGSREVIDSYDLRRGWWALFSSASSFNDLGFSLIPSSFVPMNRNIFLLLISSLF 546
Query: 574 IVIGNTGFPIFLRFVIWILFKLSPDLSLRKDSLGFLLDHPRRCFTMLFPKAATWWLFLIL 633
I+ GNTGFP F R IW +KL P +K+++ FLLDHPRRCFT+LFP ATW LF +L
Sbjct: 547 IIAGNTGFPCFFRTFIWTTYKLYPFSFEKKEAMAFLLDHPRRCFTLLFPSGATWVLFFVL 606
Query: 634 IGMNAXXXXXXXXXXXNTDVVKDLTAGFKVLNGLFQAVSTRTAGFAVLDLSQLHPXXXXX 693
+ +N + V L G +V+N +FQ+V TRTAGF + +S+LHP
Sbjct: 607 LLLNVIDLVLFMVLDTGSKAVASLPKGIRVVNAIFQSVCTRTAGFTSVSISELHPAVLVS 666
Query: 694 XXXXXXXXXXPLAISIRRTNVYEEQSLGVYGNGENQEDDGSEKNTKSFIGAHLRRQLSFD 753
P+AI++R TNVYEE+SLGVY ++D+G KSF+ HL QLS+D
Sbjct: 667 YMVMMYISVYPVAINMRNTNVYEERSLGVY---RTEDDEG-----KSFLKDHLTEQLSYD 718
Query: 754 LWYMFLGLFIICICEGGKIQDPSKPSFTVFQCLFEVVSAYGTVGLSLGYPGSNQSFSGQF 813
LWY+FLGLFIICICEGGKI +P F++F LFEVVSAYGTVGLS G SN S S +F
Sbjct: 719 LWYIFLGLFIICICEGGKISNPLDTDFSIFTVLFEVVSAYGTVGLSTGLSSSNCSLSARF 778
Query: 814 TTISKLVIIAMLIRGRHRGLPYTLDRAIILPSAKLKENDLLHELTHSRR 862
TTISKLVIIA+ +RGRHRGLP +DRAI+LPS K N+L E + RR
Sbjct: 779 TTISKLVIIALELRGRHRGLPRAVDRAILLPSEK---NNLKEEEDYQRR 824
Score = 87.0 bits (214), Expect = 1e-16
Identities = 44/101 (43%), Positives = 65/101 (63%), Gaps = 4/101 (3%)
Query: 61 LLCSILIYP---VKNFAYIDILFLATGAATQGGLNTVDLNELSLYQQIVLYITTALTTPI 117
++ SIL++ YID LFLA+ A TQ GLN+VDLN LS++QQ +LY TA+T PI
Sbjct: 32 IIASILLFTGGTTTKIKYIDALFLASSATTQTGLNSVDLNSLSIWQQFILYGFTAITVPI 91
Query: 118 FIHSGLAFVRFYWFERYF-DGIRDWSKRDFEMRRTMTLRQR 157
++H ++F+R YWF R F D +R R F+ + +L ++
Sbjct: 92 WMHGSISFIRLYWFRRKFKDVVRQNRTRKFQRKLRKSLMKK 132
>SPAC1F5.12 [P] KOG1341 Na+/K+ transporter
Length = 880
Score = 336 bits (861), Expect = 1e-91
Identities = 172/403 (42%), Positives = 245/403 (60%), Gaps = 10/403 (2%)
Query: 454 MSTNYLSYEPKIGRNSTFVGLSESQKVELGGVEYRSTKVLCLLLSIYYLGFSILAMIIFA 513
MS YLSY +GRNS F L+ ++ EL G+EY S ++L ++L +Y+L + IL ++ F
Sbjct: 452 MSLPYLSYNATVGRNSAFYALTPVEREELAGIEYESLRILTVILVVYFLFWHILGLVAFL 511
Query: 514 AWIQPMNNYKEIVRSQGVSPTWWGFWTGMSAFGNLGLTLTPNSMISFHKSIYTLIWMMWF 573
+I +V G++ WW +T S F NLG +L +S+ SF K+I+ +
Sbjct: 512 IFIYTAKTSGRVVTDGGINRGWWAAFTSSSLFDNLGYSLNSDSLNSFQKAIFPQVLGTIL 571
Query: 574 IVIGNTGFPIFLRFVIWILFKLSPDLSLRKDSLGFLLDHPRRCFTMLFPKAATWWLFLIL 633
I +GNT FPI LRF+IWI+ + + + +L FL +HPRR FT+LFP TW LFL L
Sbjct: 572 IFLGNTFFPIMLRFIIWIMIRTTRFSPNFQQALYFLFEHPRRSFTLLFPSKTTWVLFLNL 631
Query: 634 IGMNAXXXXXXXXXXXNTDVVKDLTAGFKVLNGLFQAVSTRTAGFAVLDLSQLHPXXXXX 693
+N V + G++++N +FQ +TR+AGF V+DLSQ+ P
Sbjct: 632 TLLNFASFFFFMVLDLGNSYVDKIPVGYRIMNAIFQNAATRSAGFTVVDLSQIAPAVMVT 691
Query: 694 XXXXXXXXXXPLAISIRRTNVYEEQSLGVYGNGENQEDDGSEKNT---------KSFIGA 744
P+A+SIR+TNVYEE+SLG+Y +DD + N K+F+
Sbjct: 692 YMFMMYISAYPIAMSIRQTNVYEERSLGIYAADTENDDDNNINNNNNDNNTPKRKNFLMD 751
Query: 745 HLRRQLSFDLWYMFLGLFIICICEGGKIQDPSKPSFTVFQCLFEVVSAYGTVGLSLGYPG 804
H++RQLS DLWY+FLG FII I EG +++ ++P FT+F LFEV+S YGTVGLSLGY
Sbjct: 752 HIQRQLSHDLWYLFLGYFIITIVEGRRLESEAEPQFTLFAILFEVISGYGTVGLSLGYK- 810
Query: 805 SNQSFSGQFTTISKLVIIAMLIRGRHRGLPYTLDRAIILPSAK 847
++ S + QF ISKLV++A+ IRGRHRGLP LDRA+++PS K
Sbjct: 811 NDPSLTAQFRKISKLVMVALQIRGRHRGLPSALDRAVLMPSDK 853
Score = 73.6 bits (179), Expect = 2e-12
Identities = 31/76 (40%), Positives = 51/76 (66%)
Query: 61 LLCSILIYPVKNFAYIDILFLATGAATQGGLNTVDLNELSLYQQIVLYITTALTTPIFIH 120
+LCS+++Y N YID L LA+G+ TQ GL VDL ++S+YQQ+ + + L+TPI ++
Sbjct: 39 ILCSVILYGSGNLRYIDALLLASGSCTQTGLQPVDLTQISIYQQLTILLFGVLSTPITVN 98
Query: 121 SGLAFVRFYWFERYFD 136
GL + Y++ + +D
Sbjct: 99 LGLTLFKLYFYNKRYD 114
>At4g10310 [P] KOG1341 Na+/K+ transporter
Length = 489
Score = 86.3 bits (212), Expect = 2e-16
Identities = 73/309 (23%), Positives = 144/309 (45%), Gaps = 25/309 (8%)
Query: 485 VEYRSTKVLCLLLSIYYLGFSILAMIIFAAWIQPMNNYKEIVRSQGVSPTWWGFWTGMSA 544
++ R++K L ++ Y+L +++ ++ ++ + ++++ S+ +SP + +T +S
Sbjct: 149 IDERASKCLYSVVLSYHLVTNLVGSVLLLVYVNFVKTARDVLSSKEISPLTFSVFTTVST 208
Query: 545 FGNLGLTLTPNSMISFHKSIYTLIWMMW-FIVIGNTGFPIFLRFVIWILFKLSPDLSLRK 603
F N G T +MI F K+ LIW++ +++GNT FP FL +IW L+K++ ++
Sbjct: 209 FANCGFVPTNENMIIFRKNS-GLIWLLIPQVLMGNTLFPCFLVLLIWGLYKIT-----KR 262
Query: 604 DSLGFLL-DHPRRCFTMLFPKAATWWLFLILIGMNAXXXXXXXXXXXNTDVVKDLTAGFK 662
D G++L +H + ++ L L + ++G ++ ++ +++ K
Sbjct: 263 DEYGYILKNHNKMGYSHLLSVRLCVLLGVTVLGFLIIQLLFFCAFEWTSESLEGMSSYEK 322
Query: 663 VLNGLFQAVSTRTAGFAVLDLSQLHPXXXXXXXXXXXXXXXPLAISIRRTNVYEEQSLGV 722
++ LFQ V++R G ++DLS L P L + + E++
Sbjct: 323 LVGSLFQVVNSRHTGETIVDLSTLSPAILVLFILMMYLPPYTLFMPLTEQKTIEKEG--- 379
Query: 723 YGNGENQEDDGSEKNTKSFIGAHLRRQLSFDLWYMFLGLFIICICEGGKIQ-DPSKPSFT 781
DD SE K + QLSF + + +F+I I E +Q DP +F
Sbjct: 380 -------GDDDSENGKKVKKSGLIVSQLSF----LTICIFLISITERQNLQRDPI--NFN 426
Query: 782 VFQCLFEVV 790
V EV+
Sbjct: 427 VLNITLEVI 435
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.321 0.137 0.412
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 55,182,626
Number of Sequences: 60738
Number of extensions: 2127009
Number of successful extensions: 4977
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 4956
Number of HSP's gapped (non-prelim): 12
length of query: 1069
length of database: 30,389,216
effective HSP length: 117
effective length of query: 952
effective length of database: 23,282,870
effective search space: 22165292240
effective search space used: 22165292240
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)