ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV4169 good MO KOG1183 Cell wall/membrane/envelope biogenesis N-acetylglucosaminyltransferase complex, subunit PIG-Q/GPI1, required for phosphatidylinositol biosynthesis
r_klactV4169 good MO KOG1183 Posttranslational modification, protein turnover, chaperones N-acetylglucosaminyltransferase complex, subunit PIG-Q/GPI1, required for phosphatidylinositol biosynthesis
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV4169 1438802 1440679 626
(626 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YGR216c [MO] KOG1183 N-acetylglucosaminyltransferase complex sub... 394 e-109
SPBC30D10.11 [MO] KOG1183 N-acetylglucosaminyltransferase comple... 229 7e-60
Hs4758466 [MO] KOG1183 N-acetylglucosaminyltransferase complex s... 132 2e-30
At3g57170 [MO] KOG1183 N-acetylglucosaminyltransferase complex s... 88 4e-17
CE09161 [MO] KOG1183 N-acetylglucosaminyltransferase complex sub... 66 2e-10
>YGR216c [MO] KOG1183 N-acetylglucosaminyltransferase complex subunit
PIG-Q/GPI1 required for phosphatidylinositol
biosynthesis
Length = 609
Score = 394 bits (1011), Expect = e-109
Identities = 234/626 (37%), Positives = 352/626 (55%), Gaps = 38/626 (6%)
Query: 6 YVYWPAGLLRQCKSADEPLVVIVLKTSSSDPLVIAVIEASKYRPVE--KPYSEFGRIYSG 63
Y++WP L + +A P V + + + +V+ V E V PYS +G
Sbjct: 4 YIFWPYESLFENSAAQGPQVALAISFEKTHFVVLGVCEPQYLEEVSIRPPYSVVATKNNG 63
Query: 64 NVNSIETAEQWRAEYTTIC---FDPPKVKMMQFYSLEPISLILQNPFEDFGVETTDNSAI 120
AE W + C F PK+K MQFYS +PISLI+ P ++ G+ ++ +
Sbjct: 64 -------AEGWNYKVADPCNVHFRIPKLKFMQFYSSDPISLII--PEKEVGLHSSVGETL 114
Query: 121 HDGTSVFNIRKKWESR-LRENIDHLNLYYQHLEVFYKRYAIYKN--KSTLNISITDLKLR 177
+ + R K +++ L E ++ +NL+ + + + Y + ++ + +
Sbjct: 115 NYSKLEQHPRYKRDNKKLSETLNIINLFPAYCKALNELYPFIQTSQENLRGTMLNSVAAW 174
Query: 178 MRATSVYRGIRCLAFYGAALIRIVAGFVSYVLNWRVLPLVKLFATAQQIDLRCQQLCYIP 237
+T +Y+ + + FY +I +A VS +LN+ LV A QQIDLRCQQ+CY P
Sbjct: 175 CSSTCIYKMVAKIGFYLTFVICSIASLVSSLLNYSHFQLVNYSAFVQQIDLRCQQICYFP 234
Query: 238 VQYLRINKNVKLTDLDLRTDENGSIDENDKSSGFTKRRKSHLVLPKALPCETYPDYIRLY 297
VQY RINK + ++ GS+ E D S+ + SH +P YPDYI LY
Sbjct: 235 VQYERINKKDNIQNV-------GSMVEKDNSNS----QFSHSYMPSKF----YPDYILLY 279
Query: 298 NTLWLIINDVSFGATLGTVLYENRITLAQNISTTLKYLLYELPMQVTLILEQNPLGIKLN 357
NT+WLIIND+SFG LG +L ENR L LK+ LY+ +T L NPLGIKLN
Sbjct: 280 NTIWLIINDISFGLILGAILIENRDFLVSASHRVLKFFLYDSLKTITETLANNPLGIKLN 339
Query: 358 DXXXXXXXXXXXXXXXXTYTGWIRSCIEPETIRQGITVMSHISAVIGFTFTLALAVDFLW 417
+YT +I+ I+P+T+ +T+ ++ ++GF+F ++LA+DF
Sbjct: 340 AELANFLSELFLWVIEFSYTTFIKRLIDPKTLSSLLTLTIYMMFLVGFSFAVSLAIDFFA 399
Query: 418 IMTLHISLFYMISVKLYHVHIYVLKSLFYLFYGKKQNVLRRRVDDMYFELDQLLMGTLIF 477
I++ I +FY IS KLYH + ++ SLF LF GKK+NVLR R+D YF+LDQLL+GTL+F
Sbjct: 400 ILSFPIYVFYRISSKLYHCQLNIMASLFNLFCGKKRNVLRNRIDHNYFQLDQLLLGTLLF 459
Query: 478 TVLIFLLPTLFSFYLVYTVFRFVDLTVKVLLELSLALINHFPLFALLLRIKDAKRLPGGI 537
+L+FL PT+ +FY+ YTV R + +T+++ E +ALINHFPLFALLLR+KD KRLPGGI
Sbjct: 460 IILVFLTPTVMAFYMSYTVLRMLTITIEIFSEAVIALINHFPLFALLLRLKDPKRLPGGI 519
Query: 538 ----KFKVYDDH--LLISNNPISFFMTFEPFTNVMKSIQRQMFSVETLKMVSQGSVLNVR 591
K V + H L + NNPI F F P+ ++ ++ FS T++ + +G + V
Sbjct: 520 SIELKTTVSNKHTTLELQNNPIKFKSMFRPYNLLLSQMRTNYFSFATVRKIVRGESIMVN 579
Query: 592 RDELYRVLYHNLPRHPIDIDLLWKKL 617
R++LY VLY +LP P+ + L+K+L
Sbjct: 580 RNKLYYVLYSSLPSKPLSVKDLYKRL 605
>SPBC30D10.11 [MO] KOG1183 N-acetylglucosaminyltransferase complex subunit
PIG-Q/GPI1 required for phosphatidylinositol
biosynthesis
Length = 653
Score = 229 bits (585), Expect = 7e-60
Identities = 176/572 (30%), Positives = 281/572 (48%), Gaps = 83/572 (14%)
Query: 81 ICFDPPKVKMMQFYSLEPISLILQNPFEDFGVETTD---NSAIHDGTSVFNIR------- 130
I + PP+ + MQF SLEP+SL+L +D + ++ S H + ++
Sbjct: 127 IYYHPPQPRKMQFLSLEPLSLLL---LKDSFINKSNPEYESMQHQQILLKKLKLHFPRRK 183
Query: 131 -KKWESRLRENIDHLNLYYQHLEVFYKRYAIYKNKSTLNISITDLKLRMRATSVYRGIRC 189
W+ LR + + L Q EV R ++N + N + L R+ +++ Y
Sbjct: 184 ENSWKRSLRSGL--IELLNQSFEV---RMLTHENNNKKNSYVFRLFDRVSSSTFYF-FNS 237
Query: 190 LAFYGAALIRIVAGFVSYVLNWRVLPL----VKLFATAQQIDLRCQQLCYIPVQYLRINK 245
L Y L+RI+ + +N+R +PL + +F +A+Q+DLR QQ C+ PVQY+++
Sbjct: 238 LFAYFIILLRIINEVILLAINYRPIPLSYNMMDIFVSARQVDLRLQQACFWPVQYMKL-- 295
Query: 246 NVKLTDLDLRTDENGSIDENDKSSGFTKRRKSHLVLPKALPCETYPDYIRLYNTLWLIIN 305
RKS K + E Y +YIR YN LWL+ N
Sbjct: 296 --------------------------WVFRKS-----KRVAIEDYKEYIRFYNNLWLVAN 324
Query: 306 DVSFGATLGTVLYENR---ITLAQNISTTLKYLLYELPMQVTLILEQNPLGIKLNDXXXX 362
D+ FG T+ + + EN + L +NI T +Y + + V +++ P G+KLN+
Sbjct: 325 DMIFGITMSSFILENLHLVVKLIENI--TFEYAIKNVRSMVIWLVD-TPAGLKLNNDICK 381
Query: 363 XXXXXXXXXXXXTYTGWIRSCIEPETIRQGITVMSHISAVIGFTFTLALAVDFLWIMTLH 422
++ ++ C+ P T + + IS G + +AL DFL +MT+H
Sbjct: 382 FIMKLSVWVID-VWSNFLLHCL-PWT--PFLVQVVAISGFGGASLMIALISDFLSVMTIH 437
Query: 423 ISLFYMISVKLYHVHIYVLKSLFYLFYGKKQNVLRRRVDDMYFELDQLLMGTLIFTVLIF 482
I L Y+ S +LY+ + V+ SL LF GKK+NVLR R+D ++LDQLL+GT++FTVLIF
Sbjct: 438 IHLLYLASSRLYNWQLRVIYSLLQLFRGKKRNVLRNRIDSYEYDLDQLLLGTILFTVLIF 497
Query: 483 LLPTLFSFYLVYTVFRFVDLTVKVLLELSLALINHFPLFALLLRIKDAKRLPGGIKFKVY 542
LPT++ FY + + R +T + E LA +NHFPLF +LRIKD R+P G+ F++
Sbjct: 498 FLPTIYVFYAAFALTRVSVMTCLAICETMLAFLNHFPLFVTMLRIKDPYRIPSGLNFEIV 557
Query: 543 DD-----------HLLISNNPISFFMTFEPFTNVMKSIQRQMFSVETLKMVSQG-SVLNV 590
+L ++ P+S FE + + + + S TL + G V +
Sbjct: 558 SFEPLKQDGFATLYLNCNSKPMSLGSMFEHYRKLARRLISHYLSKTTLISLLVGCPVPAI 617
Query: 591 RRDELYRVLYHNLPRHPIDI----DLLWKKLK 618
++LY + Y LP I I DLL+ + K
Sbjct: 618 PAEQLYNIQYAMLPTKRISIRKLRDLLFHQKK 649
>Hs4758466 [MO] KOG1183 N-acetylglucosaminyltransferase complex subunit
PIG-Q/GPI1 required for phosphatidylinositol
biosynthesis
Length = 581
Score = 132 bits (332), Expect = 2e-30
Identities = 70/163 (42%), Positives = 104/163 (62%), Gaps = 3/163 (1%)
Query: 399 ISAVIGFTFTLALAVDFLWIMTLHISLFYMISVKLYHVHIYVLKSLFYLFYGKKQNVLRR 458
+SA +G T L+L D + ++T HI FY+ +LY + I+ L SL+ LF GKK NVLR+
Sbjct: 375 LSACLGLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSLWRLFRGKKWNVLRQ 434
Query: 459 RVDDMYFELDQLLMGTLIFTVLIFLLPTLFSFYLVYTVFRFVDLTVKVLLELSLALINHF 518
RVD ++LDQL +GTL+FT+L+FLLPT +YLV+T+ R + + V+ L+ L + LIN
Sbjct: 435 RVDSCSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIHLLVDLINSL 494
Query: 519 PLFALLLRIKDAKRLPGGIKFKVYDDHLLISNNPISFFMTFEP 561
PL++L LR+ RL G+KF+V ++ P+ M P
Sbjct: 495 PLYSLGLRLCRPYRLAAGVKFRVLRHE---ASRPLRLLMQINP 534
>At3g57170 [MO] KOG1183 N-acetylglucosaminyltransferase complex subunit
PIG-Q/GPI1 required for phosphatidylinositol
biosynthesis
Length = 680
Score = 87.8 bits (216), Expect = 4e-17
Identities = 49/161 (30%), Positives = 91/161 (56%), Gaps = 2/161 (1%)
Query: 393 ITVMSHISAVIGFTFTLALAVDFLWIMTLHISLFYMISVKLYHVHIYVLKSLFYLFYGKK 452
I V++ + G T + A +D + TLHI + +Y I L +L+ LF G+K
Sbjct: 420 IRVIAILGITFGATVSAAFVIDVITFATLHIMALHWAITLVYSHQIQALAALWRLFRGRK 479
Query: 453 QNVLRRRVDDMYFELDQLLMGTLIFTVLIFLLPTLFSFYLVYTVFRFVDLTVKVLLELSL 512
N LR+R+D + + Q ++G+L+FT L+ LLPT FY+ +T+ ++ +L+E ++
Sbjct: 480 LNPLRQRMDSYGYTVKQHVVGSLLFTPLLLLLPTTSVFYIFFTITSTTINSICMLIEFAI 539
Query: 513 ALINHFPLFALLLRIKDAKRLPGGIKFKV--YDDHLLISNN 551
++I+ P +++ + KR P G+ F++ +H+L SN+
Sbjct: 540 SVIHATPYAEVMIWLVRRKRFPCGVWFEMEHCGEHILKSND 580
>CE09161 [MO] KOG1183 N-acetylglucosaminyltransferase complex subunit
PIG-Q/GPI1 required for phosphatidylinositol
biosynthesis
Length = 287
Score = 65.9 bits (159), Expect = 2e-10
Identities = 40/124 (32%), Positives = 63/124 (50%), Gaps = 2/124 (1%)
Query: 400 SAVIGFTFTLALAVDFLWIMTLHISLFYMISVKLYHVHIYVLKSLFYLFYGKKQNVLRRR 459
S + G + A+ DF I LH + F + KL ++ Y L L+ L GKK N LR R
Sbjct: 138 SLIGGISTFSAMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLTVLWSLVRGKKWNPLRER 197
Query: 460 VDDMYFELDQLLMGTLIFTVLIFLLPTLFSFYLVYTVFRFVDLTVKVLLELSLALINHFP 519
D + + Q + T +F +L+F+LPT+F +++V+ R ++ +L F
Sbjct: 198 KDTVILDTRQQFLATSLFVILLFILPTIFVYFVVFRCLRLAVSALQTVLYFFATW--PFQ 255
Query: 520 LFAL 523
LFAL
Sbjct: 256 LFAL 259
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.326 0.142 0.422
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 35,271,110
Number of Sequences: 60738
Number of extensions: 1436403
Number of successful extensions: 4387
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 4372
Number of HSP's gapped (non-prelim): 7
length of query: 626
length of database: 30,389,216
effective HSP length: 112
effective length of query: 514
effective length of database: 23,586,560
effective search space: 12123491840
effective search space used: 12123491840
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)