ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV4230 good EH KOG1184 Amino acid transport and metabolism Thiamine pyrophosphate-requiring enzyme
r_klactV4230 good EH KOG1184 Coenzyme transport and metabolism Thiamine pyrophosphate-requiring enzyme
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV4230 1459594 1457906 -563
(563 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YLR044c [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme 923 0.0
YLR134w [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme 872 0.0
YGR087c [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme 855 0.0
YDL080c [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme 570 e-162
SPAC3G9.11c [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme 491 e-138
YDR380w [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme 274 3e-73
At5g01320 [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme 266 8e-71
At5g01330 [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme 264 3e-70
At4g33070 [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme 260 3e-69
SPAC1F8.07c [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme 251 2e-66
At5g54960 [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme 247 3e-65
SPAC186.09 [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme 226 5e-59
SPAC13A11.06 [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme 150 6e-36
CE19658 [EH] KOG1185 Thiamine pyrophosphate-requiring enzyme 94 5e-19
CE02935 [EH] KOG1185 Thiamine pyrophosphate-requiring enzyme 94 5e-19
Hs6912418 [EH] KOG1185 Thiamine pyrophosphate-requiring enzyme 85 3e-16
YMR108w [EH] KOG4166 Thiamine pyrophosphate-requiring enzyme 78 4e-14
At3g48560 [EH] KOG4166 Thiamine pyrophosphate-requiring enzyme 75 3e-13
SPBP35G2.07 [EH] KOG4166 Thiamine pyrophosphate-requiring enzyme 72 2e-12
At5g17380 [EH] KOG1185 Thiamine pyrophosphate-requiring enzyme 69 2e-11
YEL020c [EH] KOG1185 Thiamine pyrophosphate-requiring enzyme 65 2e-10
>YLR044c [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme
Length = 563
Score = 923 bits (2385), Expect = 0.0
Identities = 458/562 (81%), Positives = 490/562 (86%)
Query: 1 MSEITLGRYLFERLKQVEVQTIFGLPGDFNLSLLDNIYEVPGMRWAGNANELNAAYAADG 60
MSEITLG+YLFERLKQV V T+FGLPGDFNLSLLD IYEV GMRWAGNANELNAAYAADG
Sbjct: 1 MSEITLGKYLFERLKQVNVNTVFGLPGDFNLSLLDKIYEVEGMRWAGNANELNAAYAADG 60
Query: 61 YARLKGMSCIITTFGVGELSALNGIAGSYAEXXXXXXXXXXXXXXXQAKQLLLHHTLGNG 120
YAR+KGMSCIITTFGVGELSALNGIAGSYAE QAKQLLLHHTLGNG
Sbjct: 61 YARIKGMSCIITTFGVGELSALNGIAGSYAEHVGVLHVVGVPSISAQAKQLLLHHTLGNG 120
Query: 121 DFTVFHRMSSNISETTAMITDINTAPAEIDRCIRTTYVSQRPVYLGLPANLVDLTVPASL 180
DFTVFHRMS+NISETTAMITDI TAPAEIDRCIRTTYV+QRPVYLGLPANLVDL VPA L
Sbjct: 121 DFTVFHRMSANISETTAMITDIATAPAEIDRCIRTTYVTQRPVYLGLPANLVDLNVPAKL 180
Query: 181 LDTPIDLSLKPNDPEAEEEVIENVLQLIKEAKNPVILADACCSRHDAKAETKKLIDLTQF 240
L TPID+SLKPND E+E+EVI+ +L L+K+AKNPVILADACCSRHD KAETKKLIDLTQF
Sbjct: 181 LQTPIDMSLKPNDAESEKEVIDTILALVKDAKNPVILADACCSRHDVKAETKKLIDLTQF 240
Query: 241 PAFVTPMGKGSIDEKHPRFGGVYVGTLSSPAVKEAVESADLVLSVGALLSDFNTGSFSYS 300
PAFVTPMGKGSIDE+HPR+GGVYVGTLS P VKEAVESADL+LSVGALLSDFNTGSFSYS
Sbjct: 241 PAFVTPMGKGSIDEQHPRYGGVYVGTLSKPEVKEAVESADLILSVGALLSDFNTGSFSYS 300
Query: 301 YKTKNIVEFHSDYTKIRSATFPGVQMKFALQKLLTKVADAAKGYKPVPVPSEPEHNEAVA 360
YKTKNIVEFHSD+ KIR+ATFPGVQMKF LQKLLT +ADAAKGYKPV VP+ N AV
Sbjct: 301 YKTKNIVEFHSDHMKIRNATFPGVQMKFVLQKLLTTIADAAKGYKPVAVPARTPANAAVP 360
Query: 361 DSTPLKQEWVWTQVGEFLREGDVVITETGTSAFGINQTHFPNNTYGISQVLWGSIXXXXX 420
STPLKQEW+W Q+G FL+EGDVVI ETGTSAFGINQT FPNNTYGISQVLWGSI
Sbjct: 361 ASTPLKQEWMWNQLGNFLQEGDVVIAETGTSAFGINQTTFPNNTYGISQVLWGSIGFTTG 420
Query: 421 XXXXXXXXXEEIDPKKRVILFIGDGSLQLTVQEISTMIRWGLKPYLFVLNNDGYTIERLI 480
EEIDPKKRVILFIGDGSLQLTVQEISTMIRWGLKPYLFVLNNDGYTIE+LI
Sbjct: 421 ATLGAAFAAEEIDPKKRVILFIGDGSLQLTVQEISTMIRWGLKPYLFVLNNDGYTIEKLI 480
Query: 481 HGETAQYNCIQNWQHLELLPTFGAKDYEAVRVSTTGEWNKLTTDEKFQDNTRIRLIEVML 540
HG AQYN IQ W HL LLPTFGAKDYE RV+TTGEW+KLT D+ F DN++IR+IE+ML
Sbjct: 481 HGPKAQYNEIQGWDHLSLLPTFGAKDYETHRVATTGEWDKLTQDKSFNDNSKIRMIEIML 540
Query: 541 PTMDAPSNLVKQAQLTAATNAK 562
P DAP NLV+QA+LTAATNAK
Sbjct: 541 PVFDAPQNLVEQAKLTAATNAK 562
>YLR134w [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme
Length = 563
Score = 872 bits (2254), Expect = 0.0
Identities = 430/562 (76%), Positives = 474/562 (83%)
Query: 1 MSEITLGRYLFERLKQVEVQTIFGLPGDFNLSLLDNIYEVPGMRWAGNANELNAAYAADG 60
MSEITLG+YLFERL QV T+FGLPGDFNLSLLD +YEV GMRWAGNANELNAAYAADG
Sbjct: 1 MSEITLGKYLFERLSQVNCNTVFGLPGDFNLSLLDKLYEVKGMRWAGNANELNAAYAADG 60
Query: 61 YARLKGMSCIITTFGVGELSALNGIAGSYAEXXXXXXXXXXXXXXXQAKQLLLHHTLGNG 120
YAR+KGMSCIITTFGVGELSALNGIAGSYAE QAKQLLLHHTLGNG
Sbjct: 61 YARIKGMSCIITTFGVGELSALNGIAGSYAEHVGVLHVVGVPSISSQAKQLLLHHTLGNG 120
Query: 121 DFTVFHRMSSNISETTAMITDINTAPAEIDRCIRTTYVSQRPVYLGLPANLVDLTVPASL 180
DFTVFHRMS+NISETTAMITDI APAEIDRCIRTTY +QRPVYLGLPANLVDL VPA L
Sbjct: 121 DFTVFHRMSANISETTAMITDIANAPAEIDRCIRTTYTTQRPVYLGLPANLVDLNVPAKL 180
Query: 181 LDTPIDLSLKPNDPEAEEEVIENVLQLIKEAKNPVILADACCSRHDAKAETKKLIDLTQF 240
L+TPIDLSLKPND EAE EV+ V++LIK+AKNPVILADAC SRHD KAETKKL+DLTQF
Sbjct: 181 LETPIDLSLKPNDAEAEAEVVRTVVELIKDAKNPVILADACASRHDVKAETKKLMDLTQF 240
Query: 241 PAFVTPMGKGSIDEKHPRFGGVYVGTLSSPAVKEAVESADLVLSVGALLSDFNTGSFSYS 300
P +VTPMGKG+IDE+HPR+GGVYVGTLS P VK+AVESADL+LS+GALLSDFNTGSFSYS
Sbjct: 241 PVYVTPMGKGAIDEQHPRYGGVYVGTLSRPEVKKAVESADLILSIGALLSDFNTGSFSYS 300
Query: 301 YKTKNIVEFHSDYTKIRSATFPGVQMKFALQKLLTKVADAAKGYKPVPVPSEPEHNEAVA 360
YKTKNIVEFHSD+ KIR+ATFPGVQMKFALQKLL + + K YKPV VP+ ++
Sbjct: 301 YKTKNIVEFHSDHIKIRNATFPGVQMKFALQKLLDAIPEVVKDYKPVAVPARVPITKSTP 360
Query: 361 DSTPLKQEWVWTQVGEFLREGDVVITETGTSAFGINQTHFPNNTYGISQVLWGSIXXXXX 420
+TP+KQEW+W +G FLREGD+VI ETGTSAFGINQT FP + Y I QVLWGSI
Sbjct: 361 ANTPMKQEWMWNHLGNFLREGDIVIAETGTSAFGINQTTFPTDVYAIVQVLWGSIGFTVG 420
Query: 421 XXXXXXXXXEEIDPKKRVILFIGDGSLQLTVQEISTMIRWGLKPYLFVLNNDGYTIERLI 480
EE+DPKKRVILFIGDGSLQLTVQEISTMIRWGLKPY+FVLNN+GYTIE+LI
Sbjct: 421 ALLGATMAAEELDPKKRVILFIGDGSLQLTVQEISTMIRWGLKPYIFVLNNNGYTIEKLI 480
Query: 481 HGETAQYNCIQNWQHLELLPTFGAKDYEAVRVSTTGEWNKLTTDEKFQDNTRIRLIEVML 540
HG A+YN IQ W HL LLPTFGA++YE RV+TTGEW KLT D+ FQDN++IR+IEVML
Sbjct: 481 HGPHAEYNEIQGWDHLALLPTFGARNYETHRVATTGEWEKLTQDKDFQDNSKIRMIEVML 540
Query: 541 PTMDAPSNLVKQAQLTAATNAK 562
P DAP NLVKQAQLTAATNAK
Sbjct: 541 PVFDAPQNLVKQAQLTAATNAK 562
>YGR087c [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme
Length = 563
Score = 855 bits (2208), Expect = 0.0
Identities = 422/562 (75%), Positives = 475/562 (84%)
Query: 1 MSEITLGRYLFERLKQVEVQTIFGLPGDFNLSLLDNIYEVPGMRWAGNANELNAAYAADG 60
MSEITLG+YLFERLKQV V TIFGLPGDFNLSLLD IYEV G+RWAGNANELNAAYAADG
Sbjct: 1 MSEITLGKYLFERLKQVNVNTIFGLPGDFNLSLLDKIYEVDGLRWAGNANELNAAYAADG 60
Query: 61 YARLKGMSCIITTFGVGELSALNGIAGSYAEXXXXXXXXXXXXXXXQAKQLLLHHTLGNG 120
YAR+KG+S ++TTFGVGELSALNGIAGSYAE QAKQLLLHHTLGNG
Sbjct: 61 YARIKGLSVLVTTFGVGELSALNGIAGSYAEHVGVLHVVGVPSISAQAKQLLLHHTLGNG 120
Query: 121 DFTVFHRMSSNISETTAMITDINTAPAEIDRCIRTTYVSQRPVYLGLPANLVDLTVPASL 180
DFTVFHRMS+NISETT+MITDI TAP+EIDR IRTT+++QRP YLGLPANLVDL VP SL
Sbjct: 121 DFTVFHRMSANISETTSMITDIATAPSEIDRLIRTTFITQRPSYLGLPANLVDLKVPGSL 180
Query: 181 LDTPIDLSLKPNDPEAEEEVIENVLQLIKEAKNPVILADACCSRHDAKAETKKLIDLTQF 240
L+ PIDLSLKPNDPEAE+EVI+ VL+LI+ +KNPVIL+DAC SRH+ K ET+KLIDLTQF
Sbjct: 181 LEKPIDLSLKPNDPEAEKEVIDTVLELIQNSKNPVILSDACASRHNVKKETQKLIDLTQF 240
Query: 241 PAFVTPMGKGSIDEKHPRFGGVYVGTLSSPAVKEAVESADLVLSVGALLSDFNTGSFSYS 300
PAFVTP+GKGSIDE+HPR+GGVYVGTLS VK+AVESADL+LSVGALLSDFNTGSFSYS
Sbjct: 241 PAFVTPLGKGSIDEQHPRYGGVYVGTLSKQDVKQAVESADLILSVGALLSDFNTGSFSYS 300
Query: 301 YKTKNIVEFHSDYTKIRSATFPGVQMKFALQKLLTKVADAAKGYKPVPVPSEPEHNEAVA 360
YKTKN+VEFHSDY K+++ATF GVQMKFALQ LL + D KGYK VPVP++ N+ V
Sbjct: 301 YKTKNVVEFHSDYVKVKNATFLGVQMKFALQNLLKVIPDVVKGYKSVPVPTKTPANKGVP 360
Query: 361 DSTPLKQEWVWTQVGEFLREGDVVITETGTSAFGINQTHFPNNTYGISQVLWGSIXXXXX 420
STPLKQEW+W ++ +FL+EGDV+I+ETGTSAFGINQT FP + YGISQVLWGSI
Sbjct: 361 ASTPLKQEWLWNELSKFLQEGDVIISETGTSAFGINQTIFPKDAYGISQVLWGSIGFTTG 420
Query: 421 XXXXXXXXXEEIDPKKRVILFIGDGSLQLTVQEISTMIRWGLKPYLFVLNNDGYTIERLI 480
EEIDP KRVILFIGDGSLQLTVQEISTMIRWGLKPYLFVLNNDGYTIE+LI
Sbjct: 421 ATLGAAFAAEEIDPNKRVILFIGDGSLQLTVQEISTMIRWGLKPYLFVLNNDGYTIEKLI 480
Query: 481 HGETAQYNCIQNWQHLELLPTFGAKDYEAVRVSTTGEWNKLTTDEKFQDNTRIRLIEVML 540
HG A+YN IQ W HL LLP FGAK YE +++TTGEW+ LTTD +FQ N+ IRLIE+ L
Sbjct: 481 HGPHAEYNEIQTWDHLALLPAFGAKKYENHKIATTGEWDALTTDSEFQKNSVIRLIELKL 540
Query: 541 PTMDAPSNLVKQAQLTAATNAK 562
P DAP +L+KQAQLTAATNAK
Sbjct: 541 PVFDAPESLIKQAQLTAATNAK 562
>YDL080c [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme
Length = 609
Score = 570 bits (1469), Expect = e-162
Identities = 267/554 (48%), Positives = 378/554 (68%), Gaps = 7/554 (1%)
Query: 4 ITLGRYLFERLKQVEVQTIFGLPGDFNLSLLDNIYEVPGMRWAGNANELNAAYAADGYAR 63
I + YLF RL Q+ + TIFGL G+F++ LLD +Y +P +RWAGN+NELNAAYAADGY+R
Sbjct: 15 IAISDYLFHRLNQLNIHTIFGLSGEFSMPLLDKLYNIPNLRWAGNSNELNAAYAADGYSR 74
Query: 64 LKGMSCIITTFGVGELSALNGIAGSYAEXXXXXXXXXXXXXXXQAKQLLLHHTLGNGDFT 123
LKG+ C+ITTFGVGELSA+NG+AGSYAE Q KQLLLHHTLGNGDFT
Sbjct: 75 LKGLGCLITTFGVGELSAINGVAGSYAEHVGILHIVGMPPTSAQTKQLLLHHTLGNGDFT 134
Query: 124 VFHRMSSNISETTAMITDINTAPAEIDRCIRTTYVSQRPVYLGLPANLVDLTVPASLLDT 183
VFHR++S+++ T +I D E+D+CI+ ++ QRPVY+G+P N V+L + ++ L+T
Sbjct: 135 VFHRIASDVACYTTLIIDSELCADEVDKCIKKAWIEQRPVYMGMPVNQVNLPIESARLNT 194
Query: 184 PIDLSLKPNDPEAEEEVIENVLQLIKEAKNPVILADACCSRHDAKAETKKLIDLTQFPAF 243
P+DL L NDP+ E+EVI +L I +++NP I+ DAC SR + ETK+L + +FP F
Sbjct: 195 PLDLQLHKNDPDVEKEVISRILSFIYKSQNPAIIVDACTSRQNLIEETKELCNRLKFPVF 254
Query: 244 VTPMGKGSIDEKHPRFGGVYVGTLSSPAVKEAVESADLVLSVGALLSDFNTGSFSYSYKT 303
VTPMGKG+++E P+FGGV+ G++S+P V+E V+ AD ++ +G +LS+F+T +F + YKT
Sbjct: 255 VTPMGKGTVNETDPQFGGVFTGSISAPEVREVVDFADFIIVIGCMLSEFSTSTFHFQYKT 314
Query: 304 KNIVEFHSDYTKIRSATFPGVQMKFALQKLLTKVADAAKGYKPVPVPSEPEHNEAVADST 363
KN +S K+++AT+P + +K LQK+L + ++ Y+P PS A +
Sbjct: 315 KNCALLYSTSVKLKNATYPDLSIKLLLQKILANLDESKLSYQPSEQPSMMVPRPYPAGNV 374
Query: 364 PLKQEWVWTQVGEFLREGDVVITETGTSAFGINQTHFPNNTYGISQVLWGSIXXXXXXXX 423
L+QEWVW ++ + + GD++ITETG SAFG+NQT FP NT GISQ LWGS+
Sbjct: 375 LLRQEWVWNEISHWFQPGDIIITETGASAFGVNQTRFPVNTLGISQALWGSVGYTMGACL 434
Query: 424 XXXXXXEEID------PKKRVILFIGDGSLQLTVQEISTMIRWGLKPYLFVLNNDGYTIE 477
+EI+ K RVILF+GDG+ QLTVQE+ST+++WGL PY+FV+NN GY+++
Sbjct: 435 GAEFAVQEINKDKFPATKHRVILFMGDGAFQLTVQELSTIVKWGLTPYIFVMNNQGYSVD 494
Query: 478 RLIHGET-AQYNCIQNWQHLELLPTFGAKDYEAVRVSTTGEWNKLTTDEKFQDNTRIRLI 536
R +H + A Y IQ W +L LL FG +YE ++ T GE+ + +D F N +IR+I
Sbjct: 495 RFLHHRSDASYYDIQPWNYLGLLRVFGCTNYETKKIITVGEFRSMISDPNFATNDKIRMI 554
Query: 537 EVMLPTMDAPSNLV 550
E+MLP D P L+
Sbjct: 555 EIMLPPRDVPQALL 568
>SPAC3G9.11c [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme
Length = 570
Score = 491 bits (1263), Expect = e-138
Identities = 269/565 (47%), Positives = 349/565 (61%), Gaps = 8/565 (1%)
Query: 3 EITLGRYLFERLKQVEVQTIFGLPGDFNLSLLDNIYEVPG--MRWAGNANELNAAYAADG 60
++ +G YLF RL Q+ +++I G+PGDFNL+LLD I +V RW GN NELN AYAAD
Sbjct: 5 KVLVGEYLFTRLLQLGIKSILGVPGDFNLALLDLIEKVGDETFRWVGNENELNGAYAADA 64
Query: 61 YARLKGMSCIITTFGVGELSALNGIAGSYAEXXXXXXXXXXXXXXXQAKQLLLHHTLGNG 120
YAR+KG+S I+TTFGVGELSALNG AG+Y+E QA + LLHHTLGNG
Sbjct: 65 YARVKGISAIVTTFGVGELSALNGFAGAYSERIPVVHIVGVPNTKAQATRPLLHHTLGNG 124
Query: 121 DFTVFHRMSSNISETTAMITDINTAPAEIDRCIRTTYVSQRPVYLGLPANLVDLTVPASL 180
DF VF RMSS +S A + ++A ID + T + RPVYL +P++ AS
Sbjct: 125 DFKVFQRMSSELSADVAFLDSGDSAGRLIDNLLETCVRTSRPVYLAVPSDAGYFYTDASP 184
Query: 181 LDTPIDLSLKPNDPEAEEEVIENVLQLIKEAKNPVILADACCSRHDAKAETKKLIDLTQF 240
L TP+ + N+ E E EV+ +L+LI+++KNP IL DAC SR + ET+ ID T F
Sbjct: 185 LKTPLVFPVPENNKEIEHEVVSEILELIEKSKNPSILVDACVSRFHIQQETQDFIDATHF 244
Query: 241 PAFVTPMGKGSIDEKHPRFGGVYVGTLSSPAVKEAVESADLVLSVGALLSDFNTGSFSYS 300
P +VTPMGK +I+E P F GVY+G+L+ P++KE ES DL+L +G L SDFN+G+F+Y+
Sbjct: 245 PTYVTPMGKTAINESSPYFDGVYIGSLTEPSIKERAESTDLLLIIGGLRSDFNSGTFTYA 304
Query: 301 YKTKNIVEFHSDYTKIRSATFPGVQMKFALQKLLTKVADAAKGYKPVPVPSEPEH---NE 357
+EFHSDYTKIRS + G+ MK L KL + + K PV EP E
Sbjct: 305 TPASQTIEFHSDYTKIRSGVYEGISMKHLLPKLTAAIDKKSVQAKARPVHFEPPKAVAAE 364
Query: 358 AVADSTPLKQEWVWTQVGEFLREGDVVITETGTSAFGINQTHFPNNTYGISQVLWGSIXX 417
A+ T + +W W FLRE DVV TETGTS FGI FP +SQVLWGSI
Sbjct: 365 GYAEGT-ITHKWFWPTFASFLRESDVVTTETGTSNFGILDCIFPKGCQNLSQVLWGSIGW 423
Query: 418 XXXXXXXXXXXXEEID-PKKRVILFIGDGSLQLTVQEISTMIRWGLKPYLFVLNNDGYTI 476
++ D P +R IL +GDGSL LTVQEIS IR GL P +FV+NN GYTI
Sbjct: 424 SVGAMFGATLGIKDSDAPHRRSILIVGDGSLHLTVQEISATIRNGLTPIIFVINNKGYTI 483
Query: 477 ERLIHGETAQYNCIQ-NWQHLELLPTFGAKDYEAVRVSTTGEWNKLTTDEKFQDNTRIRL 535
ERLIHG A YN I W + LL +GAK+ + + + E L DE+F I+L
Sbjct: 484 ERLIHGLHAVYNDINTEWDYQNLLKGYGAKNSRSYNIHSEKELLDLFKDEEFGKADVIQL 543
Query: 536 IEVMLPTMDAPSNLVKQAQLTAATN 560
+EV +P +DAP L++QA+LTA+ N
Sbjct: 544 VEVHMPVLDAPRVLIEQAKLTASLN 568
>YDR380w [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme
Length = 635
Score = 274 bits (700), Expect = 3e-73
Identities = 201/612 (32%), Positives = 297/612 (47%), Gaps = 55/612 (8%)
Query: 4 ITLGRYLFERLKQVEVQTIFGLPGDFNLSLLDNIY----EVPGMRWAGNANELNAAYAAD 59
I G Y+F+RL ++ +++FG+PGDFNLSLL+ +Y E G+RW G NELNAAYAAD
Sbjct: 25 IPFGEYIFKRLLSIDTKSVFGVPGDFNLSLLEYLYSPSVESAGLRWVGTCNELNAAYAAD 84
Query: 60 GYARLKG-MSCIITTFGVGELSALNGIAGSYAEXXXXXXXXXXXXXXXQAKQLL----LH 114
GY+R + C+ITT+GVGELSALNGIAGS+AE LH
Sbjct: 85 GYSRYSNKIGCLITTYGVGELSALNGIAGSFAENVKVLHIVGVAKSIDSRSSNFSDRNLH 144
Query: 115 HT---LGNGDFT-----VFHRM-SSNISETTAMITDINTAPAEIDRCIRTTYVSQRPVYL 165
H L + +F V+H M ++ + A + DI TA ++D IR Y +P Y+
Sbjct: 145 HLVPQLHDSNFKGPNHKVYHDMVKDRVACSVAYLEDIETACDQVDNVIRDIYKYSKPGYI 204
Query: 166 GLPANLVDLTVPA-SLLDTPI----DLSLKPNDPEAEEEVIENVLQLIKEAKNPVILADA 220
+PA+ D++V +L++ P D + P++ + + +I + I +K P IL D
Sbjct: 205 FVPADFADMSVTCDNLVNVPRISQQDCIVYPSENQLSD-IINKITSWIYSSKTPAILGDV 263
Query: 221 CCSRHDAKAETKKLIDLTQFPAFVTPMGKGSIDEKHPRFGGVYVGTLSSPAVKEAVESAD 280
R+ KLI T F T MGK IDE +P + G Y G V E E D
Sbjct: 264 LTDRYGVSNFLNKLICKTGIWNFSTVMGKSVIDESNPTYMGQYNGKEGLKQVYEHFELCD 323
Query: 281 LVLSVGALLSDFNTGSFSYSYKTK-NIVEFHSDYTKI------RSATFPGVQMKFALQKL 333
LVL G +++ N G ++++YK I++FH +Y ++ F G+ L++L
Sbjct: 324 LVLHFGVDINEINNGHYTFTYKPNAKIIQFHPNYIRLVDTRQGNEQMFKGINFAPILKEL 383
Query: 334 LTKVADAAKGYKPVPVPSEPEHNEAVADSTPLK-QEWVWTQVG------EFLREGDVVIT 386
++ D +K NE + P Q + TQV +FL GDVV+
Sbjct: 384 YKRI-DVSKLSLQYDSNVTQYTNETMRLEDPTNGQSSIITQVHLQKTMPKFLNPGDVVVC 442
Query: 387 ETGTSAFGINQTHFPNNTYGISQVLWGSIXXXXXXXXXXXXXXEEI------------DP 434
ETG+ F + FP+ ISQ + SI ++ D
Sbjct: 443 ETGSFQFSVRDFAFPSQLKYISQGFFLSIGMALPAALGVGIAMQDHSNAHINGGNVKEDY 502
Query: 435 KKRVILFIGDGSLQLTVQEISTMIRWGLKPYLFVLNNDGYTIERLIHGETAQYNCIQNWQ 494
K R+ILF GDG+ Q+T+QE+ST+++ + + + NN+GYTIER I G T YN + +W+
Sbjct: 503 KPRLILFEGDGAAQMTIQELSTILKCNIPLEVIIWNNNGYTIERAIMGPTRSYNDVMSWK 562
Query: 495 HLELLPTFGAKDYEAVRVSTTGEWNKLT--TDEKFQDNTR--IRLIEVMLPTMDAPSNLV 550
+L FG D + + +KL +E N R I L+EV L +D P L
Sbjct: 563 WTKLFEAFGDFDGKYTNSTLIQCPSKLALKLEELKNSNKRSGIELLEVKLGELDFPEQLK 622
Query: 551 KQAQLTAATNAK 562
+ A K
Sbjct: 623 CMVEAAALKRNK 634
>At5g01320 [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme
Length = 603
Score = 266 bits (679), Expect = 8e-71
Identities = 180/566 (31%), Positives = 266/566 (46%), Gaps = 11/566 (1%)
Query: 2 SEITLGRYLFERLKQVEVQTIFGLPGDFNLSLLDNIYEVPGMRWAGNANELNAAYAADGY 61
SE TLGR+L RL Q V +F +PGDFNL+LLD++ P + G NELNA YAADGY
Sbjct: 39 SESTLGRHLSRRLVQAGVTDVFSVPGDFNLTLLDHLIAEPELNNIGCCNELNAGYAADGY 98
Query: 62 ARLKGMSCIITTFGVGELSALNGIAGSYAEXXXXXXXXXXXXXXXQAKQLLLHHTLGNGD 121
AR +G+ + TF VG LS LN IAG+Y+E +LHHT+G D
Sbjct: 99 ARSRGVGACVVTFTVGGLSVLNAIAGAYSENLPVICIVGGPNSNDFGTNRILHHTIGLPD 158
Query: 122 FTVFHRMSSNISETTAMITDINTAPAEIDRCIRTTYVSQRPVYLGLPANLVDLTVPASLL 181
F+ R ++ A++ ++ A +ID+ I T +PVY+ + NL P
Sbjct: 159 FSQELRCFQTVTCYQAVVNNLEDAHEQIDKAIATALKESKPVYISISCNLAATPHPTFAR 218
Query: 182 D-TPIDLSLKPNDPEAEEEVIENVLQLIKEAKNPVILADACCSRHDAKAETKKLIDLTQF 240
D P DL+ + ++ E +E L+ + +A PV++ A +L D + +
Sbjct: 219 DPVPFDLTPRMSNTMGLEAAVEATLEFLNKAVKPVMVGGPKLRVAKASEAFLELADASGY 278
Query: 241 PAFVTPMGKGSIDEKHPRFGGVYVGTLSSPAVKEAVESADLVLSVGALLSDFNTGSFSYS 300
P V P KG + E HP F G Y G +S+P E VESAD + G + +D+++ +S
Sbjct: 279 PLAVMPSTKGLVPENHPHFIGTYWGAVSTPFCSEIVESADAYIFAGPIFNDYSSVGYSLL 338
Query: 301 YKTKNIVEFHSDYTKIRSA-TFPGVQMKFALQKLLTKVADAAKGYKPVPVPSEPEHNE-A 358
K + + H D + + TF V M ++L +V Y+ PE
Sbjct: 339 LKKEKAIIVHPDRVVVANGPTFGCVLMSDFFRELAKRVKRNETAYENYERIFVPEGKPLK 398
Query: 359 VADSTPLKQEWVWTQVGEFLREGDVVITETGTSAFGINQTHFPNNTYGISQVLWGSIXXX 418
PL+ ++ + + L VI ETG S F + P Q+ +GSI
Sbjct: 399 CKPGEPLRVNAMFQHIQKMLSSETAVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWS 458
Query: 419 XXXXXXXXXXXEEIDPKKRVILFIGDGSLQLTVQEISTMIRWGLKPYLFVLNNDGYTIER 478
+ P+KRV+ FIGDGS Q+T Q+ISTMIR G K +F++NN GYTIE
Sbjct: 459 VGATLGYA----QATPEKRVLSFIGDGSFQVTAQDISTMIRNGQKAIIFLINNGGYTIEV 514
Query: 479 LIHGETAQYNCIQNWQHLELLPTF--GAKDYEAVRVSTTGEWNKLTTDEKFQDNTRIRLI 536
IH YN I+NW + L+ G +V E + + + I
Sbjct: 515 EIH--DGPYNVIKNWNYTGLVDAIHNGEGKCWTTKVRYEEELVEAIKTATTEKKDSLCFI 572
Query: 537 EVMLPTMDAPSNLVKQAQLTAATNAK 562
EV++ D L++ +A N +
Sbjct: 573 EVIVHKDDTSKELLEWGSRVSAANGR 598
>At5g01330 [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme
Length = 592
Score = 264 bits (674), Expect = 3e-70
Identities = 182/573 (31%), Positives = 269/573 (46%), Gaps = 25/573 (4%)
Query: 2 SEITLGRYLFERLKQVEVQTIFGLPGDFNLSLLDNIYEVPGMRWAGNANELNAAYAADGY 61
S TLGR+L RL Q V IF +PGDFNLSLLD + P + G NELNA YAADGY
Sbjct: 28 SAATLGRHLSRRLVQAGVTDIFTVPGDFNLSLLDQLIANPELNNIGCCNELNAGYAADGY 87
Query: 62 ARLKGMSCIITTFGVGELSALNGIAGSYAEXXXXXXXXXXXXXXXQAKQLLLHHTLGNGD 121
AR +G+ + TF VG LS LN IAG+Y+E +LHHT+G D
Sbjct: 88 ARSRGVGACVVTFTVGGLSVLNAIAGAYSENLPVICIVGGPNSNDFGTNRILHHTIGLPD 147
Query: 122 FTVFHRMSSNISETTAMITDINTAPAEIDRCIRTTYVSQRPVYLGLPANLVDLTVPA-SL 180
F+ R ++ A++ + A +ID+ I T +PVY+ + NL + P +
Sbjct: 148 FSQELRCFQTVTCYQAVVNHLEDAHEQIDKAIATALRESKPVYISISCNLAAIPHPTFAS 207
Query: 181 LDTPIDLSLKPNDPEAEEEVIENVLQLIKEAKNPVILADACCSRHDAKAETKKLIDLTQF 240
P DL+ + ++ + E +E L+ + +A PV++ A+ +L D + +
Sbjct: 208 YPVPFDLTPRLSNKDCLEAAVEATLEFLNKAVKPVMVGGPKLRVAKARDAFVELADASGY 267
Query: 241 PAFVTPMGKGSIDEKHPRFGGVYVGTLSSPAVKEAVESADLVLSVGALLSDFNTGSFSYS 300
P V P KG + E HP F G Y G +S+ E VESAD + G + +D+++ +S
Sbjct: 268 PVAVMPSAKGFVPENHPHFIGTYWGAVSTLFCSEIVESADAYIFAGPIFNDYSSVGYSLL 327
Query: 301 YKTKNIVEFHSDYTKIRSA-TFPGVQMKFALQKLLTKVADAAKGYKPVPVPSEPEHNEAV 359
K + + H D + + TF V+M ++L AK KP E H V
Sbjct: 328 LKKEKAIIVHPDSVVVANGPTFGCVRMSEFFREL-------AKRVKPNKTAYENYHRIFV 380
Query: 360 ADSTPLKQE--------WVWTQVGEFLREGDVVITETGTSAFGINQTHFPNNTYGISQVL 411
+ PLK + ++ + + L VI ETG S F + P Q+
Sbjct: 381 PEGKPLKCKPREPLRINAMFQHIQKMLSNETAVIAETGDSWFNCQKLKLPKGCGYEFQMQ 440
Query: 412 WGSIXXXXXXXXXXXXXXEEIDPKKRVILFIGDGSLQLTVQEISTMIRWGLKPYLFVLNN 471
+GSI + P+KRV+ FIGDGS Q+T Q++STMIR G K +F++NN
Sbjct: 441 YGSIGWSVGATLGYA----QATPEKRVLSFIGDGSFQVTAQDVSTMIRNGQKTIIFLINN 496
Query: 472 DGYTIERLIHGETAQYNCIQNWQHLELLPTF--GAKDYEAVRVSTTGEWNKLTTDEKFQD 529
GYTIE IH YN I+NW + L+ G +V E + +
Sbjct: 497 GGYTIEVEIH--DGPYNVIKNWNYTGLVDAIHNGEGKCWTTKVRYEEELVEAINTATLEK 554
Query: 530 NTRIRLIEVMLPTMDAPSNLVKQAQLTAATNAK 562
+ IEV++ D L++ +A N +
Sbjct: 555 KDSLCFIEVIVHKDDTSKELLEWGSRVSAANGR 587
>At4g33070 [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme
Length = 607
Score = 260 bits (665), Expect = 3e-69
Identities = 178/566 (31%), Positives = 269/566 (47%), Gaps = 13/566 (2%)
Query: 3 EITLGRYLFERLKQVEVQTIFGLPGDFNLSLLDNIYEVPGMRWAGNANELNAAYAADGYA 62
+ TLGR+L RL Q V +F +PGDFNL+LLD++ P + G NELNA YAADGYA
Sbjct: 44 DATLGRHLARRLVQAGVTDVFSVPGDFNLTLLDHLMAEPDLNLIGCCNELNAGYAADGYA 103
Query: 63 RLKGMSCIITTFGVGELSALNGIAGSYAEXXXXXXXXXXXXXXXQAKQLLLHHTLGNGDF 122
R +G+ + TF VG LS LN IAG+Y+E +LHHT+G DF
Sbjct: 104 RSRGVGACVVTFTVGGLSVLNAIAGAYSENLPLICIVGGPNSNDYGTNRILHHTIGLPDF 163
Query: 123 TVFHRMSSNISETTAMITDINTAPAEIDRCIRTTYVSQRPVYLGLPANLVDLTVPASLLD 182
+ R ++ A++ +++ A +ID+ I T +PVY+ + NL + D
Sbjct: 164 SQELRCFQTVTCYQAVVNNLDDAHEQIDKAISTALKESKPVYISVSCNLAAIPHHTFSRD 223
Query: 183 TPIDLSLKP--NDPEAEEEVIENVLQLIKEAKNPVILADACCSRHDAKAETKKLIDLTQF 240
P+ SL P ++ E +E L+ + +A PV++ A +L D + +
Sbjct: 224 -PVPFSLAPRLSNKMGLEAAVEATLEFLNKAVKPVMVGGPKLRVAKACDAFVELADASGY 282
Query: 241 PAFVTPMGKGSIDEKHPRFGGVYVGTLSSPAVKEAVESADLVLSVGALLSDFNTGSFSYS 300
+ P KG + E HP F G Y G +S+P E VESAD + G + +D+++ +S
Sbjct: 283 ALAMMPSAKGFVPEHHPHFIGTYWGAVSTPFCSEIVESADAYIFAGPIFNDYSSVGYSLL 342
Query: 301 YKTKNIVEFHSD-YTKIRSATFPGVQMKFALQKLLTKVADAAKGYKPVPVPSEPEHNEAV 359
K + + D T TF + M ++L +V Y+ PE
Sbjct: 343 LKKEKAIVVQPDRITVANGPTFGCILMSDFFRELSKRVKRNETAYENYHRIFVPEGKPLK 402
Query: 360 ADS-TPLKQEWVWTQVGEFLREGDVVITETGTSAFGINQTHFPNNTYGISQVLWGSIXXX 418
+S PL+ ++ + + L VI ETG S F + P Q+ +GSI
Sbjct: 403 CESREPLRVNTMFQHIQKMLSSETAVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWS 462
Query: 419 XXXXXXXXXXXEEIDPKKRVILFIGDGSLQLTVQEISTMIRWGLKPYLFVLNNDGYTIER 478
+ P+KRV+ FIGDGS Q+TVQ+ISTM+R G K +F++NN GYTIE
Sbjct: 463 VGATLGYA----QASPEKRVLAFIGDGSFQVTVQDISTMLRNGQKTIIFLINNGGYTIEV 518
Query: 479 LIHGETAQYNCIQNWQHLELLPTF--GAKDYEAVRVSTTGEWNKLTTDEKFQDNTRIRLI 536
IH YN I+NW + L+ G + +V E + T + + I
Sbjct: 519 EIH--DGPYNVIKNWNYTGLVDAIHNGEGNCWTAKVRYEEELVEAITTATTEKKDCLCFI 576
Query: 537 EVMLPTMDAPSNLVKQAQLTAATNAK 562
EV+L D L++ +A N++
Sbjct: 577 EVILHKDDTSKELLEWGSRVSAANSR 602
>SPAC1F8.07c [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme
Length = 594
Score = 251 bits (641), Expect = 2e-66
Identities = 183/571 (32%), Positives = 268/571 (46%), Gaps = 34/571 (5%)
Query: 2 SEITLGRYLFERLKQVEVQTIFGLPGDFNLSLLDNIYEVPGMRWAGNANELNAAYAADGY 61
S +T+G YL +RL ++ ++ F +PGD+NL LLD + PG+ G NELN A+AA+GY
Sbjct: 7 STMTVGTYLAQRLVEIGIKNHFVVPGDYNLRLLDFLEYYPGLSEIGCCNELNCAFAAEGY 66
Query: 62 ARLKGMSCIITTFGVGELSALNGIAGSYAEXXXXXXXXXXXXXXXQAKQLLLHHTLGNGD 121
AR G++C + T+ VG L+A +GI G+YAE + LLHHTLG D
Sbjct: 67 ARSNGIACAVVTYSVGALTAFDGIGGAYAENLPVILVSGSPNTNDLSSGHLLHHTLGTHD 126
Query: 122 FTVFHRMSSNISETTAMITDINTAPAEIDRCIRTTYVSQRPVYLGLPANLVDLTVPASLL 181
F ++ ++ I AP ID IR + +PVY+ +P N+ + P +
Sbjct: 127 FEYQMEIAKKLTCAAVAIKRAEDAPVMIDHAIRQAILQHKPVYIEIPTNMANQPCP---V 183
Query: 182 DTPIDLSLKP--NDPEAEEEVIENVLQLIKEAKNPVILADACCSRHDAKAETKKLIDLTQ 239
PI + P +D E+ E+ + +LI + + P++LA A++ KL +
Sbjct: 184 PGPISAVISPEISDKESLEKATDIAAELISKKEKPILLAGPKLRAAGAESAFVKLAEALN 243
Query: 240 FPAFVTPMGKGSIDEKHPRFGGVYVGTLSSPAVKEAV-ESADLVLSVGALLSDFNTGSFS 298
AF+ P KG E+H + GVY G +SS +AV ES+DLV+ G L +D++T +
Sbjct: 244 CAAFIMPAAKGFYSEEHKNYAGVYWGEVSSSETTKAVYESSDLVIGAGVLFNDYSTVGWR 303
Query: 299 YSYKTKNIVEFHSDYT--KIRSATFPGVQMKFALQKLLTKVA---------DAAKGYKPV 347
+ NI+ +SDYT I F V M L+ L KV+ + A+ V
Sbjct: 304 -AAPNPNIL-LNSDYTSVSIPGYVFSRVYMAEFLELLAKKVSKKPATLEAYNKARPQTVV 361
Query: 348 PVPSEPEHNEAVADSTPLKQEWVWTQVGEFLREGDVVITETGTSAFGINQTHFPNNTYGI 407
P +EP+ L + V Q+ + + ETG S F Q P
Sbjct: 362 PKAAEPK--------AALNRVEVMRQIQGLVDSNTTLYAETGDSWFNGLQMKLPAGAKFE 413
Query: 408 SQVLWGSIXXXXXXXXXXXXXXEEIDPKKRVILFIGDGSLQLTVQEISTMIRWGLKPYLF 467
++ WG I P++R I+ +GDGS QLT QEIS MIR L +F
Sbjct: 414 VEMQWGHIGWSVPSAMGYAVAA----PERRTIVMVGDGSFQLTGQEISQMIRHKLPVLIF 469
Query: 468 VLNNDGYTIERLIHGETAQYNCIQNWQHLELLPTFGAKDYEAVRV-STTGEWNKLTTDEK 526
+LNN GYTIE IH YN IQNW + + +A + + TGE
Sbjct: 470 LLNNRGYTIEIQIH--DGPYNRIQNWDFAAFCESLNGETGKAKGLHAKTGEELTSAIKVA 527
Query: 527 FQDNTRIRLIEVMLPTMDAPSNLVKQAQLTA 557
Q+ LIE + T D LV + A
Sbjct: 528 LQNKEGPTLIECAIDTDDCTQELVDWGKAVA 558
>At5g54960 [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme
Length = 607
Score = 247 bits (631), Expect = 3e-65
Identities = 174/571 (30%), Positives = 259/571 (44%), Gaps = 23/571 (4%)
Query: 3 EITLGRYLFERLKQVEVQTIFGLPGDFNLSLLDNIYEVPGMRWAGNANELNAAYAADGYA 62
+ TLGRYL RL ++ V +F +PGDFNL+LLD++ P ++ G NELNA YAADGYA
Sbjct: 44 DATLGRYLARRLVEIGVTDVFSVPGDFNLTLLDHLIAEPNLKLIGCCNELNAGYAADGYA 103
Query: 63 RLKGMSCIITTFGVGELSALNGIAGSYAEXXXXXXXXXXXXXXXQAKQLLLHHTLGNGDF 122
R +G+ + TF VG LS LN IAG+Y+E +LHHT+G DF
Sbjct: 104 RSRGVGACVVTFTVGGLSVLNAIAGAYSENLPLICIVGGPNSNDYGTNRILHHTIGLPDF 163
Query: 123 TVFHRMSSNISETTAMITDINTAPAEIDRCIRTTYVSQRPVYLGLPANLVDLTVPA-SLL 181
T R ++ A+I ++ A ID I T +PVY+ + NL + +P S
Sbjct: 164 TQELRCFQAVTCFQAVINNLEEAHELIDTAISTALKESKPVYISISCNLPAIPLPTFSRH 223
Query: 182 DTPIDLSLKPNDPEAEEEVIENVLQLIKEAKNPVILADACCSRHDAKAETKKLIDLTQFP 241
P L +K ++ + +E + + +A PV++ A +L D + +
Sbjct: 224 PVPFMLPMKVSNQIGLDAAVEAAAEFLNKAVKPVLVGGPKMRVAKAADAFVELADASGYG 283
Query: 242 AFVTPMGKGSIDEKHPRFGGVYVGTLSSPAVKEAVESADLVLSVGALLSDFNTGSFSYSY 301
V P KG + E H F G Y G +S+ E VESAD L G + +D+++ +S
Sbjct: 284 LAVMPSAKGQVPEHHKHFIGTYWGAVSTAFCAEIVESADAYLFAGPIFNDYSSVGYSLLL 343
Query: 302 KTKNIVEFHSDYTKI-RSATFPGVQMKFALQKLLTKVADAAKGYK-------PVPVPSEP 353
K + + D I F V MK L +L ++ Y+ P P
Sbjct: 344 KKEKAIIVQPDRVTIGNGPAFGCVLMKDFLSELAKRIKHNNTSYENYHRIYVPEGKPLRD 403
Query: 354 EHNEAVADSTPLKQEWVWTQVGEFLREGDVVITETGTSAFGINQTHFPNNTYGISQVLWG 413
NE+ L+ ++ + L V+ ETG S F + P Q+ +G
Sbjct: 404 NPNES------LRVNVLFQHIQNMLSSESAVLAETGDSWFNCQKLKLPEGCGYEFQMQYG 457
Query: 414 SIXXXXXXXXXXXXXXEEIDPKKRVILFIGDGSLQLTVQEISTMIRWGLKPYLFVLNNDG 473
SI + P +RVI IGDGS Q+T Q++STMIR G K +F++NN G
Sbjct: 458 SIGWSVGATLGYA----QAMPNRRVIACIGDGSFQVTAQDVSTMIRCGQKTIIFLINNGG 513
Query: 474 YTIERLIHGETAQYNCIQNWQHLELLPTF--GAKDYEAVRVSTTGEWNKLTTDEKFQDNT 531
YTIE IH YN I+NW + + G +V E K ++
Sbjct: 514 YTIEVEIH--DGPYNVIKNWNYTAFVEAIHNGEGKCWTAKVRCEEELVKAINTATNEEKE 571
Query: 532 RIRLIEVMLPTMDAPSNLVKQAQLTAATNAK 562
IEV++ D L++ +A N++
Sbjct: 572 SFCFIEVIVHKDDTSKELLEWGSRVSAANSR 602
>SPAC186.09 [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme
Length = 572
Score = 226 bits (577), Expect = 5e-59
Identities = 161/569 (28%), Positives = 257/569 (44%), Gaps = 27/569 (4%)
Query: 5 TLGRYLFERLKQVEVQTIFGLPGDFNLSLLDNIYEVPGMRWAGNANELNAAYAADGYARL 64
T+G YL RL Q V+ F +PGD+NL LLD + + ANELN A+AA+GYAR
Sbjct: 15 TIGHYLAVRLAQAGVKHHFVVPGDYNLGLLDKLQYNNYLEEVNCANELNCAFAAEGYARA 74
Query: 65 KGMSCIITTFGVGELSALNGIAGSYAEXXXXXXXXXXXXXXXQAKQLLLHHTLGNGDFTV 124
G++ + T+ VG +A +GI G+YAE LLHHTLG DF+
Sbjct: 75 NGIAACVVTYSVGAFTAFDGIGGAYAEDLPVILISGSPNTNDIGSSHLLHHTLGTHDFSY 134
Query: 125 FHRMSSNISETTAMITDINTAPAEIDRCIRTTYVSQRPVYLGLPANLVDLTV----PASL 180
+ M+ I+ I AP ID I+ + ++PVY+ +P N+ PASL
Sbjct: 135 QYEMAKKITCAAVSIQRPTEAPRLIDYAIKMALLKKKPVYIEVPTNVASQPCAAPGPASL 194
Query: 181 LDTPIDLSLKPNDPEAEEEVIENVLQLIKEAKNPVILADACCSRHDAKAETKKLIDLTQF 240
+ P + ++ E + ++ +++ + PV+LA A++ +L +
Sbjct: 195 ITEP-----ETSNQEYLQMAVDISAKIVNGKQKPVLLAGPKLRSFKAESAFLELANSLNC 249
Query: 241 PAFVTPMGKGSIDEKHPRFGGVYVGTLSSPAVKEAVESADLVLSVGALLSDFNTGSFSYS 300
V P K E HP + G+Y G S+ + + +D ++ G +D+++ ++
Sbjct: 250 SVAVMPNAKSFFPESHPNYAGIYWGQASTLGAESIINWSDCIICAGTTFTDYSSNGWTSL 309
Query: 301 YKTKNIVEFHSDYTKIRSATFPGVQMKFALQKLLTKVA---DAAKGYKPVPVPSEPEHNE 357
N++ D + A F GV ++ L +L KV + YK + PE E
Sbjct: 310 PPKANVLHVDVDRVTVSDAEFGGVLLRDFLHELAKKVKANNASVVEYKRI----RPESLE 365
Query: 358 AVADS--TPLKQEWVWTQVGEFLREGDVVITETGTSAFGINQTHFPNNTYGISQVLWGSI 415
++ L ++ + QV + + + +TG S FG + P ++ WG I
Sbjct: 366 IPMENPKAALNRKEIIRQVQNLVNQETTLFVDTGDSWFGGMRITLPEKARFEIEMQWGHI 425
Query: 416 XXXXXXXXXXXXXXEEIDPKKRVILFIGDGSLQLTVQEISTMIRWGLKPYLFVLNNDGYT 475
PK+ V++F+GDGS Q TVQE+S M+R L +F++NN GYT
Sbjct: 426 GWSVPSAFGYAIGA----PKRNVVVFVGDGSFQETVQEVSQMVRLNLPIIMFLINNRGYT 481
Query: 476 IERLIHGETAQYNCIQNWQHLELLPTFGAKDYEA--VRVSTTGEWNKLTTDEKFQDNTRI 533
IE IH YN I+NW + ++ F A + A RV E + K +++
Sbjct: 482 IEVEIH--DGPYNRIKNWDYAAIVEAFNAGEGHAKGFRVGNGHELAEAIRQAK-ENSQGP 538
Query: 534 RLIEVMLPTMDAPSNLVKQAQLTAATNAK 562
LIE + D L+ A N K
Sbjct: 539 TLIECNIDQDDCSKELINWGHNVGAANGK 567
>SPAC13A11.06 [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme
Length = 152
Score = 150 bits (378), Expect = 6e-36
Identities = 79/149 (53%), Positives = 99/149 (66%), Gaps = 2/149 (1%)
Query: 3 EITLGRYLFERLKQVEVQTIFGLPGDFNLSLLDNIYEVPG--MRWAGNANELNAAYAADG 60
+I +G YLF+RL+Q+ V++I G+PGDFNL+LLD I +V RW GN NELN AYAADG
Sbjct: 4 DILVGEYLFKRLEQLGVKSILGVPGDFNLALLDLIEKVGDEKFRWVGNTNELNGAYAADG 63
Query: 61 YARLKGMSCIITTFGVGELSALNGIAGSYAEXXXXXXXXXXXXXXXQAKQLLLHHTLGNG 120
YAR+ G+S I+TTFGVGELSA+NG+AGSYAE Q LLHHTLG+G
Sbjct: 64 YARVNGLSAIVTTFGVGELSAINGVAGSYAEHVPVVHIVGMPSTKVQDTGALLHHTLGDG 123
Query: 121 DFTVFHRMSSNISETTAMITDINTAPAEI 149
DF F M +S + MI + N A +I
Sbjct: 124 DFRTFMDMFKKVSAYSIMIDNGNDAAEKI 152
>CE19658 [EH] KOG1185 Thiamine pyrophosphate-requiring enzyme
Length = 634
Score = 94.0 bits (232), Expect = 5e-19
Identities = 112/487 (22%), Positives = 196/487 (39%), Gaps = 53/487 (10%)
Query: 14 LKQVEVQTIFGLPGDFNLSLLDNIYEVPGMRWAGNANELNAAYAADGYARLKGMS-CIIT 72
LK V+ +FG+ G F + + + G+++ G NE AAYAA L G ++
Sbjct: 82 LKSQGVEYMFGVVG-FPVIEVGMAAQAHGIKYIGCRNEQAAAYAAQAMGYLTGKPVALLV 140
Query: 73 TFGVGELSALNGIAGSYAEXXXXXXXXXXXXXXXQAKQLLLHHTLGNGDFTVFHRMSS-- 130
G G L A+ G+A + + + G F + + S
Sbjct: 141 VSGPGILHAIGGLANATVNCWPVVCIGGTADVDLENR----------GAFQEWSQQESVR 190
Query: 131 NISETTAMITDINTAPAEIDRCIRTT-YVSQRPVYLGLPANLVDLTVPASLLDTP-IDLS 188
N + + T ++T PA I++ +R Y VY+ LP NLV + + P + L
Sbjct: 191 NSCKHVSRPTSLHTIPAHIEKAVRCAMYGRPGAVYVDLPGNLVLTSTEEEISFPPQVPLP 250
Query: 189 LKPNDPEAEEEVIENVLQLIKEAKNPVILADACCSRHDAKA-ETKKLIDLTQFPAFVTPM 247
+ P E IE + +K AK P+++ + + A + ++ + ++ P TP
Sbjct: 251 APVSIPPIAE--IEKAIDTLKSAKKPLVIVGKGAAWSERGATQVQQFLTKSKLPWLATPG 308
Query: 248 GKGSIDEKHPRFGGVYVGTLSSPAVKEAVESADLVLSVGA----LLSDFNTGSFSYSYKT 303
GKG + HPRF +G S A++E AD V +GA +L F K
Sbjct: 309 GKGVASDLHPRF----IGQARSLALRE----ADTVFLIGARFNWILHFGLPPRFQKDVKV 360
Query: 304 KNI----VEFHSDYTKIRSATFPGVQMKFALQKLLTKVAD-----AAKGYKPVPVPSEPE 354
I EFH + +++ + L +L ++ D + + +K + E
Sbjct: 361 VQIDICPEEFHQN---VKTEVPLLGDIGETLAELTPRLGDWTYDESTEWFK--KLRENAE 415
Query: 355 HNEAVAD------STPLKQEWVWTQVGEFLREGDVVITETGTSAFGINQTHFPNN--TYG 406
N A + STPL + + EFL DV++ G + I +T P+
Sbjct: 416 KNRAAVEKFVDDHSTPLNYYAAYQPIREFLANNDVIVINEGANTMDIGRTMMPSRLPKRR 475
Query: 407 ISQVLWGSIXXXXXXXXXXXXXXEEIDPKKRVILFIGDGSLQLTVQEISTMIRWGLKPYL 466
+ +G++ + PK +V++ GD + + E+ T+ R+ L
Sbjct: 476 LDAGTFGTMGVGHGFSLAAALWARDHSPKTKVLVVQGDSAFGFSAMELETIARYNLPVVT 535
Query: 467 FVLNNDG 473
++NN G
Sbjct: 536 VIINNSG 542
>CE02935 [EH] KOG1185 Thiamine pyrophosphate-requiring enzyme
Length = 634
Score = 94.0 bits (232), Expect = 5e-19
Identities = 112/487 (22%), Positives = 196/487 (39%), Gaps = 53/487 (10%)
Query: 14 LKQVEVQTIFGLPGDFNLSLLDNIYEVPGMRWAGNANELNAAYAADGYARLKGMS-CIIT 72
LK V+ +FG+ G F + + + G+++ G NE AAYAA L G ++
Sbjct: 82 LKSQGVEYMFGVVG-FPVIEVGMAAQAHGIKYIGCRNEQAAAYAAQAMGYLTGKPVALLV 140
Query: 73 TFGVGELSALNGIAGSYAEXXXXXXXXXXXXXXXQAKQLLLHHTLGNGDFTVFHRMSS-- 130
G G L A+ G+A + + + G F + + S
Sbjct: 141 VSGPGILHAIGGLANATVNCWPVVCIGGTADVDLENR----------GAFQEWSQQESVR 190
Query: 131 NISETTAMITDINTAPAEIDRCIRTT-YVSQRPVYLGLPANLVDLTVPASLLDTP-IDLS 188
N + + T ++T PA I++ +R Y VY+ LP NLV + + P + L
Sbjct: 191 NSCKHVSRPTSLHTIPAHIEKAVRCAMYGRPGAVYVDLPGNLVLTSTEEEISFPPQVPLP 250
Query: 189 LKPNDPEAEEEVIENVLQLIKEAKNPVILADACCSRHDAKA-ETKKLIDLTQFPAFVTPM 247
+ P E IE + +K AK P+++ + + A + ++ + ++ P TP
Sbjct: 251 APVSIPPIAE--IEKAIDTLKSAKKPLVIVGKGAAWSERGATQVQQFLTKSKLPWLATPG 308
Query: 248 GKGSIDEKHPRFGGVYVGTLSSPAVKEAVESADLVLSVGA----LLSDFNTGSFSYSYKT 303
GKG + HPRF +G S A++E AD V +GA +L F K
Sbjct: 309 GKGVASDLHPRF----IGQARSLALRE----ADTVFLIGARFNWILHFGLPPRFQKDVKV 360
Query: 304 KNI----VEFHSDYTKIRSATFPGVQMKFALQKLLTKVAD-----AAKGYKPVPVPSEPE 354
I EFH + +++ + L +L ++ D + + +K + E
Sbjct: 361 VQIDICPEEFHQN---VKTEVPLLGDIGETLAELTPRLGDWTYDESTEWFK--KLRENAE 415
Query: 355 HNEAVAD------STPLKQEWVWTQVGEFLREGDVVITETGTSAFGINQTHFPNN--TYG 406
N A + STPL + + EFL DV++ G + I +T P+
Sbjct: 416 KNRAAVEKFVDDHSTPLNYYAAYQPIREFLANNDVIVINEGANTMDIGRTMMPSRLPKRR 475
Query: 407 ISQVLWGSIXXXXXXXXXXXXXXEEIDPKKRVILFIGDGSLQLTVQEISTMIRWGLKPYL 466
+ +G++ + PK +V++ GD + + E+ T+ R+ L
Sbjct: 476 LDAGTFGTMGVGHGFSLAAALWARDHSPKTKVLVVQGDSAFGFSAMELETIARYNLPVVT 535
Query: 467 FVLNNDG 473
++NN G
Sbjct: 536 VIINNSG 542
>Hs6912418 [EH] KOG1185 Thiamine pyrophosphate-requiring enzyme
Length = 578
Score = 85.1 bits (209), Expect = 3e-16
Identities = 120/573 (20%), Positives = 233/573 (39%), Gaps = 61/573 (10%)
Query: 3 EITLGRYLFERLKQVEVQTIFGLPGDFNLSLLDNIYEVPGMRWAGNANELNAAYAAD--G 60
+++ + + + LK +V+ IFG+ G ++ + + G+++ G NE A YAA G
Sbjct: 13 QVSGAKVIAQALKTQDVEYIFGIVG-IPVTEIAIAAQQLGIKYIGMRNEQAACYAASAIG 71
Query: 61 YARLKGMSCIITTFGVGELSALNGIAGSYAEXXXXXXXXXXXXXXXQAKQLLLHHTLGNG 120
Y + C++ + G G + AL G+A + + G
Sbjct: 72 YLTSRPGVCLVVS-GPGLIHALGGMANANMNCWPLLVIGGSSERNQETM----------G 120
Query: 121 DFTVFHRMSSN--ISETTAMITDINTAPAEIDRCIRTT-YVSQRPVYLGLPANLVDLTVP 177
F F ++ + ++ +A + I P I++ +R++ Y Y+ +PA+ V+L V
Sbjct: 121 AFQEFPQVEACRLYTKFSARXSSIEAIPFVIEKAVRSSIYGRPGACYVDIPADFVNLQVN 180
Query: 178 ASLLDTPIDLSLKPNDPEAEEEVIENVLQLIKEAKNPVILADACCSRHDAKAETKKLIDL 237
+ + ++ + P AE + +I+ AK P+++ + A+ KKL++
Sbjct: 181 VNSIKY-MERCMSPPISMAETSAVCTAASVIRNAKQPLLIIGKGAAYAHAEESIKKLVEQ 239
Query: 238 TQFPAFVTPMGKGSIDEKHPRFGGVYVGTLSSPAVKEAVESADLVLSVGALLSDFNTGSF 297
+ P TPMGKG + + HP G A A++ AD+++ GA L+
Sbjct: 240 YKLPFLPTPMGKGVVPDNHPYCVGA--------ARSRALQFADVIVLFGARLNWILHFGL 291
Query: 298 SYSYKTK-NIVEFHSDYTKIRSATFPGVQMK---FALQKLLTKVADAAKGYKP------V 347
Y+ ++ ++ + P V + A+ K L + D P
Sbjct: 292 PPRYQPDVKFIQVDICAEELGNNVKPAVTLLGNIHAVTKQLLEELDKTPWQYPPESKWWK 351
Query: 348 PVPSEPEHNEAVA------DSTPLKQEWVWTQVGEFLREGDVVITETGTSAFGINQTHFP 401
+ + + NEA + S P+ V+ V E L V++E G + I +T
Sbjct: 352 TLREKMKSNEAASKELASKKSLPMNYYTVFYHVQEQLPRDCFVVSE-GANTMDIGRTVLQ 410
Query: 402 N--NTYGISQVLWGSIXXXXXXXXXXXXXXEEIDPKKRVILFIGDGSLQLTVQEISTMIR 459
N + + +G++ ++ P +I GD + + E+ T+ R
Sbjct: 411 NYLPRHRLDAGTFGTMGVGLGFAIAAAVVAKDRSPGHWIICVEGDSAFGFSGMEVETICR 470
Query: 460 WGLKPYLFVLNNDGY-------TIERLIHGETA-----QYNCIQNWQHLELLPTFGAKDY 507
+ L L V+NN+G T + ++ + A + N + +++ FG K Y
Sbjct: 471 YNLPIILLVVNNNGIYQGFDTDTWKEMLKFQDATAVVPPMCLLPNSHYEQVMTAFGGKGY 530
Query: 508 EAVRVSTTGEWNKLTTDEKFQDNTRIRLIEVML 540
V T E K + ++ D T+ LI +M+
Sbjct: 531 ---FVQTPEELQK-SLEQSLADTTKPSLINIMI 559
>YMR108w [EH] KOG4166 Thiamine pyrophosphate-requiring enzyme
Length = 687
Score = 77.8 bits (190), Expect = 4e-14
Identities = 114/514 (22%), Positives = 194/514 (37%), Gaps = 72/514 (14%)
Query: 4 ITLGRYLFERLKQVEVQTIFGLPGDFNLSLLDNIYEVPGMRWAGNANELNAAYAADGYAR 63
+T G+ E + + V T+FG PG L + D I+ + +E A + A+GYAR
Sbjct: 92 LTGGQIFNEMMSRQNVDTVFGYPGGAILPVYDAIHNSDKFNFVLPKHEQGAGHMAEGYAR 151
Query: 64 LKGM-SCIITTFGVGELSALNGIAGSYAEXXXXXXXXXXXXXXXQAKQLLLHHTLGNGDF 122
G ++ T G G + + +A ++A+ +
Sbjct: 152 ASGKPGVVLVTSGPGATNVVTPMADAFADGIPMVVFTGQVPTSAIGTDAFQEADVVG--- 208
Query: 123 TVFHRMSSNISETTAMITDINTAPAEIDRCIR-TTYVSQRPVYLGLPANLVDLTVPASLL 181
+S + ++ M+ + P I+ T PV + LP + V A++L
Sbjct: 209 -----ISRSCTKWNVMVKSVEELPLRINEAFEIATSGRPGPVLVDLPKD-----VTAAIL 258
Query: 182 DTPIDL-------SLKPNDPEAEEEV----IENVLQLIKEAKNPVILADACCSRH-DAKA 229
PI +L A++E I LI AK PV+ A H D
Sbjct: 259 RNPIPTKTTLPSNALNQLTSRAQDEFVMQSINKAADLINLAKKPVLYVGAGILNHADGPR 318
Query: 230 ETKKLIDLTQFPAFVTPMGKGSIDEKHPRFGGVYVGTLSSPAVKEAVESADLVLSVGALL 289
K+L D Q P T G GS D++ P+ + +G AV++ADL+++VGA
Sbjct: 319 LLKELSDRAQIPVTTTLQGLGSFDQEDPKSLDM-LGMHGCATANLAVQNADLIIAVGARF 377
Query: 290 SDFNTGSFS-YSYKTK--------NIVEFHSDYTKIRSATFPGVQMKFALQ-KLLTKVAD 339
D TG+ S ++ + + I+ F I VQ + A++ T +
Sbjct: 378 DDRVTGNISKFAPEARRAAAEGRGGIIHFEVSPKNINKV----VQTQIAVEGDATTNLGK 433
Query: 340 AAKGYKPVPVPSE---------PEHNEAVADSTP---LKQEWVWTQVGEFLRE-GDVVIT 386
PV SE E+ A + TP +K + V ++ + + G VI
Sbjct: 434 MMSKIFPVKERSEWFAQINKWKKEYPYAYMEETPGSKIKPQTVIKKLSKVANDTGRHVIV 493
Query: 387 ETGTSAFGI--------NQTHFPNNTYGISQVLWGSIXXXXXXXXXXXXXXEEIDPKKRV 438
TG + H + G+ + +G + P+ V
Sbjct: 494 TTGVGQHQMWAAQHWTWRNPHTFITSGGLGTMGYG---------LPAAIGAQVAKPESLV 544
Query: 439 ILFIGDGSLQLTVQEISTMIRWGLKPYLFVLNND 472
I GD S +T+ E+S+ ++ G + +LNN+
Sbjct: 545 IDIDGDASFNMTLTELSSAVQAGTPVKILILNNE 578
>At3g48560 [EH] KOG4166 Thiamine pyrophosphate-requiring enzyme
Length = 670
Score = 75.1 bits (183), Expect = 3e-13
Identities = 112/486 (23%), Positives = 193/486 (39%), Gaps = 47/486 (9%)
Query: 10 LFERLKQVEVQTIFGLPGDFNLSLLDNIYEVPGMRWAGNANELNAAYAADGYARLKGMSC 69
L E L++ V+T+F PG ++ + + +R +E +AA+GYAR G
Sbjct: 103 LVEALERQGVETVFAYPGGASMEIHQALTRSSSIRNVLPRHEQGGVFAAEGYARSSGKPG 162
Query: 70 I-ITTFGVGELSALNGIAGSYAEXXXXXXXXXXXXXXXQAKQLLLHHTLGNGDF--TVFH 126
I I T G G + ++G+A + + + +G F T
Sbjct: 163 ICIATSGPGATNLVSGLADALLDSVPLVAITGQ----------VPRRMIGTDAFQETPIV 212
Query: 127 RMSSNISETTAMITDINTAPAEIDRC-IRTTYVSQRPVYLGLPANL-VDLTVP--ASLLD 182
++ +I++ ++ D+ P I+ T PV + +P ++ L +P +
Sbjct: 213 EVTRSITKHNYLVMDVEDIPRIIEEAFFLATSGRPGPVLVDVPKDIQQQLAIPNWEQAMR 272
Query: 183 TPIDLSLKPNDPEAEEEVIENVLQLIKEAKNPVILADACCSRHDAKAETKKLIDLTQFPA 242
P +S P P E+ +E +++LI E+K PV+ C ++ E + ++LT P
Sbjct: 273 LPGYMSRMPKPP--EDSHLEQIVRLISESKKPVLYVGGGCL--NSSDELGRFVELTGIPV 328
Query: 243 FVTPMGKGSI---DEKHPRFGGVYVGTLSSPAVKEAVESADLVLSVGALLSDFNTGSFSY 299
T MG GS DE G++ GT+ + AVE +DL+L+ G D TG
Sbjct: 329 ASTLMGLGSYPCDDELSLHMLGMH-GTVYA---NYAVEHSDLLLAFGVRFDDRVTGKLEA 384
Query: 300 SYKTKNIVEFHSDYTKIRSATFPGV----QMKFALQKLLTKVADAAKGYK---------- 345
IV D +I P V +K ALQ + + + A+ K
Sbjct: 385 FASRAKIVHIDIDSAEIGKNKTPHVSVCGDVKLALQGMNKVLENRAEELKLDFGVWRNEL 444
Query: 346 PVPVPSEPEHNEAVADSTPLKQEWVWTQVGEFLREGDVVITETGTSAFGINQTHFPNNTY 405
V P + ++ P + +V + L +G +I+ TG + F N
Sbjct: 445 NVQKQKFPLSFKTFGEAIPPQYA---IKVLDELTDGKAIIS-TGVGQHQMWAAQFYNYKK 500
Query: 406 GISQVLWGSIXXXXXXXXXXXXXXEEIDPKKRVILFIGDGSLQLTVQEISTMIRWGLKPY 465
+ G + +P V+ GDGS + VQE++T+ L
Sbjct: 501 PRQWLSSGGL-GAMGFGLPAAIGASVANPDAIVVDIDGDGSFIMNVQELATIRVENLPVK 559
Query: 466 LFVLNN 471
+ +LNN
Sbjct: 560 VLLLNN 565
>SPBP35G2.07 [EH] KOG4166 Thiamine pyrophosphate-requiring enzyme
Length = 669
Score = 72.4 bits (176), Expect = 2e-12
Identities = 111/510 (21%), Positives = 187/510 (35%), Gaps = 63/510 (12%)
Query: 5 TLGRYLFERLKQVEVQTIFGLPGDFNLSLLDNIYEVPGMRWAGNANELNAAYAADGYARL 64
T G + + + V+ +FG PG L + D IY P + +E A +AA Y+R+
Sbjct: 88 TGGEIFHDMMLKHNVKHVFGYPGGAILPVFDAIYRSPHFEFILPRHEQAAGHAAQAYSRV 147
Query: 65 -KGMSCIITTFGVGELSALNGIAGSYAEXXXXXXXXXXXXXXXQAKQLLLHHTLGNGDFT 123
K ++ T G G + + IA + A+ +
Sbjct: 148 TKKPGVVLVTSGPGATNVITPIADALADGTPLVVFSGQVATSAIGSDAFQEADMVG---- 203
Query: 124 VFHRMSSNISETTAMITDINTAPAEIDRCIR-TTYVSQRPVYLGLPANLVDLTVPASLLD 182
+S + ++ M+ D+ P ID T PV + LP + V AS+L
Sbjct: 204 ----ISRSCTKWNVMVKDVADLPRRIDEAFEIATSGRPGPVLVDLPKD-----VTASVLK 254
Query: 183 TPID-LSLKPNDPEAEEEVIE-----------NVLQLIKEAKNPVIL-ADACCSRHDAKA 229
PI LS P+ +EV+E V L+K AK PVI + +
Sbjct: 255 EPIPILSSVPSMNRRMKEVLEEGSKNVTAKIDRVANLLKLAKKPVIFCGHGVLANPECPT 314
Query: 230 ETKKLIDLTQFPAFVTPMGKGSIDEKHPRFGGVYVGTLSSPAVKEAVESADLVLSVGALL 289
+K + Q P + +G G++DE+ +G S A++ ADL+L++G
Sbjct: 315 LLRKFSERLQIPVTTSLLGLGAVDERSD-LSLHMLGMHGSGYANMAMQEADLILALGVRF 373
Query: 290 SDFNTGSFSY---------SYKTKNIVEFHSDYTKIRSATFPGVQMKFALQKLLTKVADA 340
D TG+ S + + I+ F I P ++ + + L + A
Sbjct: 374 DDRVTGNVSLFAPQARLAAAEERGGIIHFDISPKNIGKVVQPTEAIEGDVYESLKLLDSA 433
Query: 341 AKGYKPVPVPSEPEHNEAVADSTPLKQEWVWT----QVGEFLREGDV------------- 383
K K P + ++ K+ + +T GE ++ +V
Sbjct: 434 TKNIK------IPSRFDWLSQIQTWKERFPFTFTRSAPGELVKPQEVIQELDKQTSDIKD 487
Query: 384 -VITETGTSAFGINQTHFPNNTYGISQVLWGSIXXXXXXXXXXXXXXEEIDPKKRVILFI 442
V TG A + F T S V G + PK VI
Sbjct: 488 KVTITTGVGAHQMWAATFYRWTKPSSLVTSGGLGTMGFGLPAAIGASVAA-PKDIVIDID 546
Query: 443 GDGSLQLTVQEISTMIRWGLKPYLFVLNND 472
GD S +T E++T+ ++ + + +LNN+
Sbjct: 547 GDASFSMTGMELATVRQFDIPVKILILNNE 576
>At5g17380 [EH] KOG1185 Thiamine pyrophosphate-requiring enzyme
Length = 572
Score = 68.6 bits (166), Expect = 2e-11
Identities = 71/286 (24%), Positives = 121/286 (41%), Gaps = 29/286 (10%)
Query: 14 LKQVEVQTIFGLPGDFNLSLLDNIYEVPGMRWAGNANELNAAYAADGYARLKGM-SCIIT 72
L + V +FG+ G SL + G+R+ NE +A YAA Y L G ++T
Sbjct: 22 LSHLGVTHMFGVVGIPVTSLASRAMAL-GIRFIAFHNEQSAGYAASAYGYLTGKPGILLT 80
Query: 73 TFGVGELSALNGIAGSYAEXXXXXXXXXXXXXXXQAKQLLLHHTLGNGDFTVFHRMSS-- 130
G G + L G++ ++ +G GDF ++ +
Sbjct: 81 VSGPGCVHGLAGLSNAWVNTWPMVMISGSCD----------QRDVGRGDFQELDQIEAVK 130
Query: 131 NISETTAMITDINTAPAEIDRCIRTTYVSQRP--VYLGLPANLVDLTVPASLLDTPIDLS 188
S+ + D+ P + R + VS RP YL +P +++ + S D +D
Sbjct: 131 AFSKLSEKAKDVREIPDCVSRVLDRA-VSGRPGGCYLDIPTDVLRQKISESEADKLVDEV 189
Query: 189 LKPNDPE----AEEEVIENVLQLIKEAKNPVILADACCSRHDAKAETKKLIDLTQFPAFV 244
+ E + IE+ + L+++A+ P+I+ + A+ E KKL+++T P
Sbjct: 190 ERSRKEEPIRGSLRSEIESAVSLLRKAERPLIVFGKGAAYSRAEDELKKLVEITGIPFLP 249
Query: 245 TPMGKGSIDEKHPRFGGVYVGTLSSPAVKEAVESADLVLSVGALLS 290
TPMGKG + + H F +L A+ D+ L VGA L+
Sbjct: 250 TPMGKGLLPDTH-EFSATAARSL-------AIGKCDVALVVGARLN 287
>YEL020c [EH] KOG1185 Thiamine pyrophosphate-requiring enzyme
Length = 560
Score = 65.5 bits (158), Expect = 2e-10
Identities = 112/531 (21%), Positives = 207/531 (38%), Gaps = 52/531 (9%)
Query: 5 TLGRYLFERLKQVEVQTIFGLPGDFNLSLLDNIYEVPGMRWAGNANELNAAYAADGYARL 64
T ++ + L++ + T+FG+ G + L D + G+++ NE A+YAA Y +
Sbjct: 4 TATQHFAQLLQKYGIDTVFGIVGIPIVQLADTMV-ANGIKFIPCRNEQAASYAASAYGYI 62
Query: 65 KGMSCIITTFGVGELSALNGIAGSYAEXXXXXXXXXXXXXXXQAKQLLLHHTLGNGDFTV 124
++ VG ++ +AG Y Q+ + G F
Sbjct: 63 SDKPGVLLI--VGGPGLIHALAGIYNSMSNRWPLLVIAGSSSQSD-------IHKGGFQE 113
Query: 125 FHRMS--SNISETTAMITDINT---APAEIDRCIR-TTYVSQRPVYLGLPANLVDLTVPA 178
++S S + T +T N ++ CI+ T VS Y+ +PA+ ++ P
Sbjct: 114 LDQVSLLSPFLKFTGKLTPDNIDMITQKALNYCIQGTAGVS----YIDVPADFIEYEKPL 169
Query: 179 SLLD---TPIDLSLKPNDPEAEEEVIENVLQLIKEAKNPVILADACCSRHDAKAETKKLI 235
D + + L PN + I+ V+QLI + KN IL E ++L+
Sbjct: 170 EGNDRTGNELPMILTPNICGPDPSKIKKVVQLILQHKNKNILIVIGKGAVKNSHEIRRLV 229
Query: 236 DLTQFPAFVTPMGKGSIDEKHPRFGGVYVGTLSSPAVKEAVESADLVLSVGALLSDFNTG 295
+ P TPM KG + + P S A +A++ AD+VL +GA L+
Sbjct: 230 NTFNLPFLPTPMAKGIVPDSSP--------LNVSSARSQALKIADIVLVLGARLNWILHF 281
Query: 296 SFSYSYKTKNI-VEFHSD-YTKIRSATFPGVQMK------FALQKLLTKVADAAKGYKPV 347
S + +++I ++F S+ T + PG + ++ L+ ++ +K
Sbjct: 282 GTSPKWNSESIFIQFDSNPETLGDNNVSPGADLSIWGDIGLSVTALVEELTRQDSCWKYS 341
Query: 348 PVPSEPEHNEAVADSTPLKQE----------WVWTQVGEFLREGDVVITETGTSAFGINQ 397
V E + + L++E V+ + + + ++ G + I +
Sbjct: 342 GVKQEIREKIQLNQTRLLRKEKTRGAQLNYNQVYGTLRPLIDDYRTILVTEGANTMDIAR 401
Query: 398 THFPNNTYGISQVLWGSIXXXXXXXXXXXXXXEEIDPKKRVILFIGDGSLQLTVQEISTM 457
FP T + L + P+ V+L GD + + EI T
Sbjct: 402 ISFP--TDAPRRRLDAGTNATMGIGLGYALACKASHPELDVVLIQGDSAFGFSAMEIETA 459
Query: 458 IRWGLKPYLFVLNNDG-YTIERLIHGETAQYNCIQNWQHLELLPTFGAKDY 507
+R L + V+NN G Y E+ I G+ +N ++ + GA D+
Sbjct: 460 VRCQLALVIVVMNNSGIYHGEKDIEGDLPPTALSKNCRYDLVGKGLGANDF 510
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.134 0.389
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 31,179,766
Number of Sequences: 60738
Number of extensions: 1290977
Number of successful extensions: 3317
Number of sequences better than 1.0e-05: 21
Number of HSP's better than 0.0 without gapping: 13
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 3254
Number of HSP's gapped (non-prelim): 30
length of query: 563
length of database: 30,389,216
effective HSP length: 112
effective length of query: 451
effective length of database: 23,586,560
effective search space: 10637538560
effective search space used: 10637538560
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)