ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactV4230.1    suspect: Pn  R     KOG1901   General function prediction only                               Uncharacterized high-glucose-regulated protein

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactV4230.1 1458827  1459402 192  
         (192 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

7301251 [R] KOG1901 Uncharacterized high-glucose-regulated protein     31   1.2  
YMR004w [U] KOG2273 Membrane coat complex Retromer subunit VPS5/...    28   5.9  
SPCC736.08 [K] KOG3743 Recombination signal binding protein-J ka...    28   7.7  

>7301251 [R] KOG1901 Uncharacterized high-glucose-regulated protein
          Length = 700

 Score = 30.8 bits (68), Expect = 1.2
 Identities = 28/125 (22%), Positives = 51/125 (40%), Gaps = 22/125 (17%)

Query: 74  SVQQRS--WDSQVDQVSWQTQVDWSLGNVSGSDTSVDFS-------------WGSVDIG- 117
           SV++R+  W  QVD+ S++     +   VSG+D S+ F              W +++ G 
Sbjct: 16  SVEERNIPWSQQVDEASYENLSSPTHDEVSGNDNSMQFQYPPFNFKENCNTPWNNMNKGR 75

Query: 118 ----DHSSGFRNVGGHSVENSEXXXXXXXXXXXLLSLRRDGWNTDNVQDTNVFSVRTGNT 173
               +H S  R+  GHS+ N+                    +  +N Q +     RT + 
Sbjct: 76  KANANHDSYRRH--GHSIPNNTRRDEHQVNPWNSRKPAAQSFRNENDQPSTKLDNRTSDE 133

Query: 174 VQSRQ 178
            Q+++
Sbjct: 134 AQNQE 138


>YMR004w [U] KOG2273 Membrane coat complex Retromer subunit VPS5/SNX1
           Sorting nexins and related PX domain-containing proteins
          Length = 511

 Score = 28.5 bits (62), Expect = 5.9
 Identities = 14/51 (27%), Positives = 24/51 (46%), Gaps = 1/51 (1%)

Query: 73  WSVQQRSWDSQVDQVSWQTQVDWSLGNVSGSDTSVDFSWGSVDIGDHSSGF 123
           W+    +W    D V   T  + SL  +SG   +++FS   +D  +  +GF
Sbjct: 10  WNTSSNAWTKDDDHVVSTTNSEPSLNGISGEFNTLNFS-TPLDTNEEDTGF 59


>SPCC736.08 [K] KOG3743 Recombination signal binding protein-J kappa(CBF1 Su(H)
           HS2NF5)
          Length = 613

 Score = 28.1 bits (61), Expect = 7.7
 Identities = 15/45 (33%), Positives = 25/45 (55%), Gaps = 5/45 (11%)

Query: 95  WSLGNVSGSDTSVDFSWGSVDIGDHSSGFRN----VGGHSVENSE 135
           W   N+SGS+TS   +   +++ + ++G  N     GG + ENSE
Sbjct: 7   WDFANISGSNTSGSLNLNQLNLDNINNGLHNQEDGAGGRN-ENSE 50


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.315    0.130    0.416 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,626,744
Number of Sequences: 60738
Number of extensions: 310835
Number of successful extensions: 546
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 546
Number of HSP's gapped (non-prelim): 3
length of query: 192
length of database: 30,389,216
effective HSP length: 100
effective length of query: 92
effective length of database: 24,315,416
effective search space: 2237018272
effective search space used: 2237018272
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)