ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV4272 good I KOG3016 Lipid transport and metabolism Acyl-CoA thioesterase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV4272 1470356 1471357 334
(334 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YJR019c [I] KOG3016 Acyl-CoA thioesterase 312 5e-85
CE27071 [I] KOG3016 Acyl-CoA thioesterase 87 4e-17
CE27070 [I] KOG3016 Acyl-CoA thioesterase 87 4e-17
CE07132 [I] KOG3016 Acyl-CoA thioesterase 86 8e-17
CE27834 [I] KOG3016 Acyl-CoA thioesterase 83 5e-16
Hs4885565 [I] KOG3016 Acyl-CoA thioesterase 83 6e-16
CE28719 [I] KOG3016 Acyl-CoA thioesterase 72 9e-13
CE04023 [I] KOG3016 Acyl-CoA thioesterase 58 2e-08
>YJR019c [I] KOG3016 Acyl-CoA thioesterase
Length = 349
Score = 312 bits (799), Expect = 5e-85
Identities = 165/343 (48%), Positives = 227/343 (66%), Gaps = 16/343 (4%)
Query: 1 MNKLHSLLRLQQVSLTRYRTVSKPKAPPGGRGTFGGILVSQSLLASLQTIPVDFNPSSLH 60
M+ L +L L +S T + T P AP G +GTFGG LVSQSLLASL T+P++F P+SLH
Sbjct: 8 MSNLEKILELVPLSPTSFVTKYLPAAPVGSKGTFGGTLVSQSLLASLHTVPLNFFPTSLH 67
Query: 61 CYFLIGGNPDHDIDYDVESLRSGKNFIHKQVKAYQFDKLIFISTILF--YRQPDPDKLHA 118
YF+ GG+P I Y V++LR+G+NFIHKQV AYQ DKLIF S ILF R + D L
Sbjct: 68 SYFIKGGDPRTKITYHVQNLRNGRNFIHKQVSAYQHDKLIFTSMILFAVQRSKEHDSLQH 127
Query: 119 LNRL-GKLPKLPDFKKFRESTQLFENQVMG--KLPNFKRVSTVFQGSDAETKKLRDRFET 175
+ G K PD ++ E+T LF+ +V+ KL + +S FQ + + +K + D F+
Sbjct: 128 WETIPGLQGKQPDPHRYEEATSLFQKEVLDPQKLSRYASLSDRFQDATSMSKYV-DAFQY 186
Query: 176 GTVDYRFPDNFFVNESDSDELDYYLRVRENIPASGVPGFSPNA----------DISPEND 225
G ++Y+FP + F + +DELDY+++VR I G + I+PEND
Sbjct: 187 GVMEYQFPKDMFYSARHTDELDYFVKVRPPITTVEHAGDESSLHKHHPYRIPKSITPEND 246
Query: 226 VRFNYVAFAYLSDSYVLLSLPFFHNLPMYSHKFSVSLDHTIHFHQLPKVNDWMFAKLFNP 285
R+NYVAFAYLSDSY+LL++P+FHNLP+Y H FSVSLDHTI+FHQLP VN+W++ K+ NP
Sbjct: 247 ARYNYVAFAYLSDSYLLLTIPYFHNLPLYCHSFSVSLDHTIYFHQLPHVNNWIYLKISNP 306
Query: 286 RSHHDKHLILGHYYERKSRELVATISQEGLVAYPEKQTILALF 328
RSH DKHL+ G Y++ +S ++A++SQEG V Y ++ I A F
Sbjct: 307 RSHWDKHLVQGKYFDTQSGRIMASVSQEGYVVYGSERDIRAKF 349
>CE27071 [I] KOG3016 Acyl-CoA thioesterase
Length = 346
Score = 86.7 bits (213), Expect = 4e-17
Identities = 72/287 (25%), Positives = 133/287 (46%), Gaps = 25/287 (8%)
Query: 34 FGGILVSQSLLASLQTIPVDFNPSSLHCYFLIGGNPDHDIDYDVESLRSGKNFIHKQVKA 93
+GG++ SQ+L A+ +T+ F P S+H YF++ + I Y+V +R G++FI + V+A
Sbjct: 71 YGGLIFSQALAAAEKTVDEQFKPHSMHSYFILNVDTKEPISYNVRRIRDGRSFITRTVEA 130
Query: 94 YQFDKLIFISTILFYRQPDPDKLHALNRLGKLPKLPDFKKFRESTQLFENQV-MGKLPNF 152
Q DK+ F+ F+ + +H + + K+P+ R++ + V G++
Sbjct: 131 VQKDKVCFVLQCSFHVEEKSSIIHQ-SEMPKVPEPEVLMSMRDAVPYMKELVEKGEVTPP 189
Query: 153 KRVSTVFQGSDAET-KKLRDRFETGTVDYRFPDNFFVNESD-SDELDYYLRVRENIPASG 210
+ Q D++ +D FE + N++ SD EL +++R R ++
Sbjct: 190 PAMLARLQSYDSKVYSDDQDLFEMRCTNL---GNYYGFASDKKPELYFWMRARGDL---- 242
Query: 211 VPGFSPNADISPENDVRFNYVAFAYLSDSYVLLSLPFFHNLPMYSHKFSVSLDHTIHFHQ 270
ND RF+ AY SDS ++ + H + SLDH + FH+
Sbjct: 243 ------------SNDERFHRWLIAYNSDSLLVSTAVSPHYTTGFCSSMLFSLDHCVWFHR 290
Query: 271 LP-KVNDWMFAKLFNPRSHHDKHLILGHYYERKSRELVATISQEGLV 316
K ++W+ + + + + I G + R+ L+A+ QE LV
Sbjct: 291 SEVKADEWLLFECKSRIASGSRATIEGRIW-RRDGVLIASCQQEALV 336
>CE27070 [I] KOG3016 Acyl-CoA thioesterase
Length = 357
Score = 86.7 bits (213), Expect = 4e-17
Identities = 72/287 (25%), Positives = 133/287 (46%), Gaps = 25/287 (8%)
Query: 34 FGGILVSQSLLASLQTIPVDFNPSSLHCYFLIGGNPDHDIDYDVESLRSGKNFIHKQVKA 93
+GG++ SQ+L A+ +T+ F P S+H YF++ + I Y+V +R G++FI + V+A
Sbjct: 82 YGGLIFSQALAAAEKTVDEQFKPHSMHSYFILNVDTKEPISYNVRRIRDGRSFITRTVEA 141
Query: 94 YQFDKLIFISTILFYRQPDPDKLHALNRLGKLPKLPDFKKFRESTQLFENQV-MGKLPNF 152
Q DK+ F+ F+ + +H + + K+P+ R++ + V G++
Sbjct: 142 VQKDKVCFVLQCSFHVEEKSSIIHQ-SEMPKVPEPEVLMSMRDAVPYMKELVEKGEVTPP 200
Query: 153 KRVSTVFQGSDAET-KKLRDRFETGTVDYRFPDNFFVNESD-SDELDYYLRVRENIPASG 210
+ Q D++ +D FE + N++ SD EL +++R R ++
Sbjct: 201 PAMLARLQSYDSKVYSDDQDLFEMRCTNL---GNYYGFASDKKPELYFWMRARGDL---- 253
Query: 211 VPGFSPNADISPENDVRFNYVAFAYLSDSYVLLSLPFFHNLPMYSHKFSVSLDHTIHFHQ 270
ND RF+ AY SDS ++ + H + SLDH + FH+
Sbjct: 254 ------------SNDERFHRWLIAYNSDSLLVSTAVSPHYTTGFCSSMLFSLDHCVWFHR 301
Query: 271 LP-KVNDWMFAKLFNPRSHHDKHLILGHYYERKSRELVATISQEGLV 316
K ++W+ + + + + I G + R+ L+A+ QE LV
Sbjct: 302 SEVKADEWLLFECKSRIASGSRATIEGRIW-RRDGVLIASCQQEALV 347
>CE07132 [I] KOG3016 Acyl-CoA thioesterase
Length = 343
Score = 85.9 bits (211), Expect = 8e-17
Identities = 86/336 (25%), Positives = 142/336 (41%), Gaps = 45/336 (13%)
Query: 4 LHSLLRLQQVSLTRYRTVSKPKAPPGGRGTFGGILVSQSLLASLQTIPVDFNPSSLHCYF 63
+ + L LQ++ Y K +GG +V QSL A+ T+ F P SLH YF
Sbjct: 33 IDTFLNLQRIDTNLYIARHLLKGRHSYNAVYGGQVVGQSLAAAAATVEDCFIPHSLHSYF 92
Query: 64 LIGGNPDHDIDYDVESLRSGKNFIHKQVKAYQFDKLIFISTILFYR-------------- 109
+ G+ D I Y ++ +R G++F + VKA Q + IF I F+
Sbjct: 93 IKTGSVDKPILYMIDRIRDGRSFCTRVVKAVQDGEAIFSCQISFHHVCWGNGTENKKRDT 152
Query: 110 -QPDPDKLHALNRLGKLPKLPDFKKFRESTQLFENQVMGKLPNFKRVSTVFQGSDAE-TK 167
Q +PD A+ K+P++ ++ L + ++ K V V G K
Sbjct: 153 FQKEPD---AIKHSSKMPEVTPPEQL-----LPAREAALEVLRTKEVDEVTAGVIQHFLK 204
Query: 168 KLRDRFETGTVDYR--FPDNFFVNESDSDELDYYLRVRENIPASGVPGFSPNADISPEND 225
++ D FE D R P + + E ++R REN+ +D
Sbjct: 205 EIPDAFER-VFDVRPVNPAKYLLKEDTEPMSMIWIRARENL----------------GDD 247
Query: 226 VRFNYVAFAYLSDSYVLLSLPFFHNLPMYSHKFSVSLDHTIHFHQLP-KVNDWMFAKLFN 284
R + AYL+D +L + H + S SLDH I H+ +++DWM + +
Sbjct: 248 HRLHQCVAAYLTDLSMLTTAVRPHIRNGFIPSMSFSLDHCIWMHENEFRIDDWMLYETIS 307
Query: 285 PRSHHDKHLILGHYYERKSRELVATISQEGLVAYPE 320
++ + I G + R R +++T +QE LV P+
Sbjct: 308 SKAGGSRAFIEGRLWSRDGRLIIST-AQEALVRAPK 342
>CE27834 [I] KOG3016 Acyl-CoA thioesterase
Length = 414
Score = 83.2 bits (204), Expect = 5e-16
Identities = 82/324 (25%), Positives = 144/324 (44%), Gaps = 34/324 (10%)
Query: 4 LHSLLRLQQVSLTRYRTVSKPKAPPGGRGTFGGILVSQSLLASLQTIPVDFNPSSLHCYF 63
+ + L L++V Y K +GG ++ Q+L A+ T+ V F P+SLH YF
Sbjct: 101 IDTFLNLERVDKNLYLARHLLKGRNSLPVVYGGQVIGQALSAATATVEVGFVPNSLHSYF 160
Query: 64 LIGGNPDHDIDYDVESLRSGKNFIHKQVKAYQFDKLIFISTILFYRQPDPDKLHALNRLG 123
+ GN + I Y V+ +R GK+F + VKA Q + IF I F+R +H L
Sbjct: 161 VQSGNVERPILYQVDRIRDGKSFCTRLVKALQDGEAIFTVQISFHRPEADSIVHQL---- 216
Query: 124 KLPKLPDFKKFRESTQLFENQVMGKLPNFKRVSTVFQGSDAETKKLRDRFETGTVDYRFP 183
+P++P + + FE + K N + G + K++ F
Sbjct: 217 PMPEVPAPDSLEDLSDTFER--IKKNANIPPAALAMIG--FKQKEIPPAF---------- 262
Query: 184 DNFFVNESDSDELDYYLRVRENIPASG----------VPGFSPNADISPENDVRFNYVAF 233
F + ++D YL ++++ +G N +I +D R + A
Sbjct: 263 --FRIFSFRPVDIDSYLCLKKDDHTAGHGHPTDAYRSYVWIKANENIG--DDPRLHLAAA 318
Query: 234 AYLSDSYVLLSLPFFHNLPMYSHKFSVSLDHTIHFH-QLPKVNDWMFAKLFNPRSHHDKH 292
AY+SD+ ++ + H+ + +++LDH+I H +V+DWM + + + +
Sbjct: 319 AYISDATMIETALRPHSKRGFIPSMALTLDHSIWMHTDNFRVDDWMLYENHSTIAGGGRS 378
Query: 293 LILGHYYERKSRELVATISQEGLV 316
LI G + R R LV + +QE L+
Sbjct: 379 LIEGKLWTRDGR-LVFSTTQEALI 401
>Hs4885565 [I] KOG3016 Acyl-CoA thioesterase
Length = 319
Score = 82.8 bits (203), Expect = 6e-16
Identities = 72/291 (24%), Positives = 125/291 (42%), Gaps = 45/291 (15%)
Query: 34 FGGILVSQSLLASLQTIPVDFNPSSLHCYFLIGGNPDHDIDYDVESLRSGKNFIHKQVKA 93
FGG +V Q+L+A+ +++ D + SLHCYF+ G+P + Y VE R+G +F + VKA
Sbjct: 55 FGGQIVGQALVAAAKSVSEDVHVHSLHCYFVRAGDPKLPVLYQVERTRTGSSFSVRSVKA 114
Query: 94 YQFDKLIFISTILFYR-QPDPDKLHALNRLGKLPKLPDFKKFRESTQLFENQVMGKLPNF 152
Q K IFI F + QP P + +P +P ++ + L + + + PN
Sbjct: 115 VQHGKPIFICQASFQQAQPSP-----MQHQFSMPTVPPPEELLDCETLIDQYL--RDPNL 167
Query: 153 KRVSTVFQGSDAETKKLRDRFETGTVDYRFPDNFFVNESDSDELDYYLRVRENIPASGVP 212
++ R+P +N + E+ ++ P S +
Sbjct: 168 QK--------------------------RYP--LALNRIAAQEVPIEIKPVNPSPLSQLQ 199
Query: 213 GFSPN-------ADISPENDVRFNYVAFAYLSDSYVLLSLPFFHNLPMYSHKFSVSLDHT 265
P E D++ + AY+SD Y L + + F VSLDH+
Sbjct: 200 RMEPKQMFWVRARGYIGEGDMKMHCCVAAYISD-YAFLGTALLPHQWQHKVHFMVSLDHS 258
Query: 266 IHFHQLPKVNDWMFAKLFNPRSHHDKHLILGHYYERKSRELVATISQEGLV 316
+ FH + + WM + +P + + L+ G + + L T +QEG++
Sbjct: 259 MWFHAPFRADHWMLYECESPWAGGSRGLVHGRVWSQDG-VLAVTCAQEGVI 308
>CE28719 [I] KOG3016 Acyl-CoA thioesterase
Length = 281
Score = 72.4 bits (176), Expect = 9e-13
Identities = 69/282 (24%), Positives = 115/282 (40%), Gaps = 48/282 (17%)
Query: 34 FGGILVSQSLLASLQTIPVDFNPSSLHCYFLIGGNPDHDIDYDVESLRSGKNFIHKQVKA 93
FGG +++Q++ A+ T P F ++HCYF+ GG I Y+V+ +R G+NF + V+A
Sbjct: 30 FGGHVLAQAMSAAYYTAPEGFYVHAVHCYFIRGGEESIPITYNVKIIRDGRNFAIRYVEA 89
Query: 94 YQFDKLIFISTILFYRQPDPDKLHALNRLGKLPKLPDFKKFRESTQLFENQVMGK---LP 150
Q K+I ++ L KL K T F + V G +
Sbjct: 90 VQHGKVIHLAEF------------------SLQKLASVKDTFSLTPEFPSHVPGPDGLVS 131
Query: 151 NFK-RVSTVFQGSDAE------TKKLRDRFETGTVDYRFPDNFFVNESDSDELDYYLRVR 203
N R V +G DA T+++ E D N F++ + YL +R
Sbjct: 132 NITGRHQKVAEGVDARELRGQVTERMAPSLEIRPADL----NMFLHGTGGVNQKQYLWIR 187
Query: 204 ENIPASGVPGFSPNADISPENDVRFNYVAFAYLSDSYVLLSLPFFHNLPMYSHKFSVSLD 263
IP ++D + YLSD ++ + + M+ K S+D
Sbjct: 188 YKIPVD-------------KSDYMLRHATIVYLSDLELVTTGALCFDDKMF--KLQTSMD 232
Query: 264 HTIHFHQLP-KVNDWMFAKLFNPRSHHDKHLILGHYYERKSR 304
H+ HQ +NDW+ + + + + LI G + R +
Sbjct: 233 HSAWIHQYEFDINDWILYEQECVANSNHRSLIHGRLWSRDGK 274
>CE04023 [I] KOG3016 Acyl-CoA thioesterase
Length = 276
Score = 57.8 bits (138), Expect = 2e-08
Identities = 32/103 (31%), Positives = 55/103 (53%), Gaps = 5/103 (4%)
Query: 28 PGGRGTFGGILVSQSLLASLQTIPVDFNPSSLHCYFLIGGNPDHDIDYDVESLRSGKNFI 87
P G +GG+L SQ+L A+ T+ +F P+S+H YF+ Y V+ ++ GK FI
Sbjct: 35 PNG-SAYGGLLFSQALAAAENTVSDEFKPNSIHAYFVSAVIYSTPAIYKVKRIKDGKTFI 93
Query: 88 HKQVKAYQFDKLIFISTILFYRQPDPDKLHALNRLGKLPKLPD 130
+ V+A Q D++ F+ + F+ +H +PK+P+
Sbjct: 94 IRTVEAVQNDRICFVLQVSFHISEKSGMIHQ----DIMPKVPN 132
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.321 0.140 0.415
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,912,634
Number of Sequences: 60738
Number of extensions: 1025365
Number of successful extensions: 2191
Number of sequences better than 1.0e-05: 8
Number of HSP's better than 0.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 2175
Number of HSP's gapped (non-prelim): 10
length of query: 334
length of database: 30,389,216
effective HSP length: 107
effective length of query: 227
effective length of database: 23,890,250
effective search space: 5423086750
effective search space used: 5423086750
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)