ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV4282 good H KOG4230 Coenzyme transport and metabolism C1-tetrahydrofolate synthase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV4282 1474321 1471481 -947
(947 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YGR204w [H] KOG4230 C1-tetrahydrofolate synthase 1218 0.0
YBR084w [H] KOG4230 C1-tetrahydrofolate synthase 1131 0.0
SPBC839.16 [H] KOG4230 C1-tetrahydrofolate synthase 1097 0.0
Hs13699868 [H] KOG4230 C1-tetrahydrofolate synthase 1043 0.0
SPBC2G2.08 [H] KOG4230 C1-tetrahydrofolate synthase 1016 0.0
Hs22055576 [H] KOG4230 C1-tetrahydrofolate synthase 503 e-142
At3g12290 [H] KOG0089 Methylenetetrahydrofolate dehydrogenase/me... 298 3e-80
At4g00620 [H] KOG0089 Methylenetetrahydrofolate dehydrogenase/me... 283 9e-76
At2g38660 [H] KOG0089 Methylenetetrahydrofolate dehydrogenase/me... 263 1e-69
Hs22053503 [H] KOG4230 C1-tetrahydrofolate synthase 245 3e-64
At4g00600 [H] KOG0089 Methylenetetrahydrofolate dehydrogenase/me... 228 4e-59
CE25047 [H] KOG4230 C1-tetrahydrofolate synthase 215 2e-55
7299133 [H] KOG0089 Methylenetetrahydrofolate dehydrogenase/meth... 204 7e-52
Hs5729935 [H] KOG0089 Methylenetetrahydrofolate dehydrogenase/me... 197 8e-50
Hs13376513_2 [H] KOG0089 Methylenetetrahydrofolate dehydrogenase... 130 1e-29
Hs22040978 [H] KOG0089 Methylenetetrahydrofolate dehydrogenase/m... 102 3e-21
Hs20541728 [H] KOG0089 Methylenetetrahydrofolate dehydrogenase/m... 78 6e-14
YKR080w [H] KOG0089 Methylenetetrahydrofolate dehydrogenase/meth... 74 1e-12
Hs20542451 [H] KOG0089 Methylenetetrahydrofolate dehydrogenase/m... 69 4e-11
Hs17437830 [H] KOG0089 Methylenetetrahydrofolate dehydrogenase/m... 61 7e-09
SPBC1711.04 [H] KOG0089 Methylenetetrahydrofolate dehydrogenase/... 60 1e-08
>YGR204w [H] KOG4230 C1-tetrahydrofolate synthase
Length = 946
Score = 1218 bits (3151), Expect = 0.0
Identities = 619/951 (65%), Positives = 741/951 (77%), Gaps = 15/951 (1%)
Query: 3 IIDGKAIANGIRDEIKDEISSLKEKYAVFRPTLTIFQVGNRPDSNTYVKMKLKAAKDAGI 62
++DGKA A R I +EI S++ F P L I QVGNRPDS TYV+MK KAA++AGI
Sbjct: 5 VLDGKACAQQFRSNIANEIKSIQGHVPGFAPNLAIIQVGNRPDSATYVRMKRKAAEEAGI 64
Query: 63 DVKFIHLDETVTEVELLGQIDDENSDDSVHGILVQLPLPAHLNEDKITSRVSACKDVDGF 122
FIHLDE+ TE E+L +D N D HGI+VQLPLPAHL+ED+ITSRV A KDVDGF
Sbjct: 65 VANFIHLDESATEFEVLRYVDQLNEDPHTHGIIVQLPLPAHLDEDRITSRVLAEKDVDGF 124
Query: 123 GPVNIGELNKRNGTPYFQPCTPRGIIELLKRSGVTIAGSEAVVIGRSDIVGSPVATLLTS 182
GP NIGELNK+NG P+F PCTP+GIIELL ++ VTI GS +VVIGRSDIVGSPVA LL S
Sbjct: 125 GPTNIGELNKKNGHPFFLPCTPKGIIELLHKANVTIEGSRSVVIGRSDIVGSPVAELLKS 184
Query: 183 LDATVTILHSKSKDLREFISRADIVVVAIGQPEFIRGEWF-----ANNKRGAVVIDVGTN 237
L++TVTI HSK++D+ ++ ADIVVVAIGQPEF++GEWF ++ + VVIDVGTN
Sbjct: 185 LNSTVTITHSKTRDIASYLHDADIVVVAIGQPEFVKGEWFKPRDGTSSDKKTVVIDVGTN 244
Query: 238 FVKDETRKSGYRNVGDVEFAAAEPYVKLITPVPGGVGPMTVAMLMQNTFESAKRYLDKQL 297
+V D ++KSG++ VGDVEF A YV LITPVPGGVGPMTVAMLMQNT +AKR +++
Sbjct: 245 YVADPSKKSGFKCVGDVEFNEAIKYVHLITPVPGGVGPMTVAMLMQNTLIAAKRQMEES- 303
Query: 298 NSGNDFEPLKLNLLKPVPSDFEISRAQVPKPITQIAAESGILPSELEPYGDTKAKVKLDL 357
+ PL L LL PVPSD +ISRAQ PK I Q+A E GI ELE YG KAK+ +
Sbjct: 304 SKPLQIPPLPLKLLTPVPSDIDISRAQQPKLINQLAQELGIYSHELELYGHYKAKISPKV 363
Query: 358 LNRLKNRENGKYILVTGITPTPLGEGKSTTTVGLAQALGAHLKKKVFANVRQPSMGPTFG 417
+ RL+ R+NGKYILV+GITPTPLGEGKSTTT+GL QAL AHL K ANVRQPS+GPT G
Sbjct: 364 IERLQTRQNGKYILVSGITPTPLGEGKSTTTMGLVQALTAHLGKPAIANVRQPSLGPTLG 423
Query: 418 IKXXXXXXXYSQVIPMDEFNLHVTGDIHAISMANNLLAAAIDTRMFHESTQSN-AALYKR 476
+K YSQVIPMDEFNLH+TGDIHAI ANNLLAAAIDTRMFHE+TQ N A Y R
Sbjct: 424 VKGGAAGGGYSQVIPMDEFNLHLTGDIHAIGAANNLLAAAIDTRMFHETTQKNDATFYNR 483
Query: 477 LVPAKKGVRKFTPTMLRRLKKLGINKTDPNELSAEESARFARLDIDPETITWRRVVDCND 536
LVP K G RKFTP+M RRL +LGI KT+P++L+ EE +FARL+IDP+TIT +RVVD ND
Sbjct: 484 LVPRKNGKRKFTPSMQRRLNRLGIQKTNPDDLTPEEINKFARLNIDPDTITIKRVVDIND 543
Query: 537 RFLRGITVGQAPTERGHTRYTGFDISVASECMAVLALANSLEDMRERLGRIVIAASKSGE 596
R LR IT+GQAPTE+ HTR TGFDI+VASE MA+LAL+ L DM+ER+GR+V+AA +
Sbjct: 544 RMLRQITIGQAPTEKNHTRVTGFDITVASELMAILALSKDLRDMKERIGRVVVAADVNRS 603
Query: 597 PITCEDIGCAGAMTALLKDAIKPNIMQTLEGTPVFVHAGPFANISIGANSVLADKMALKL 656
P+T ED+GC GA+TALL+DAIKPN+MQTLEGTPV VHAGPFANISIGA+SV+AD++ALKL
Sbjct: 604 PVTVEDVGCTGALTALLRDAIKPNLMQTLEGTPVLVHAGPFANISIGASSVIADRVALKL 663
Query: 657 AGVPTDLPEDVKKTQKGYVVTEAGFDFTMGGERFINIKCRSSGLVPDVVVIVATVRALKV 716
G E KT+ GYVVTEAGFDFTMGGERF NIKCRSSGL P+ VV+VATVRALK
Sbjct: 664 VGT-----EPEAKTEAGYVVTEAGFDFTMGGERFFNIKCRSSGLTPNAVVLVATVRALKS 718
Query: 717 HGGGPEVKAGAPLPSEYLNEDVELLRKGCANLAKHISNARAYNLPVVVAINKMSSDTDNE 776
HGG P+VK G PLPS Y E++E + KG AN+ K I+N + + +PVVVAINK +DT+ E
Sbjct: 719 HGGAPDVKPGQPLPSAYTEENIEFVEKGAANMCKQIANIKQFGVPVVVAINKFETDTEGE 778
Query: 777 HEVIREESIKAGAVDAIVSNHWEEGGKGAVDLAEGIINTANNQSNEDFKFLYEVEGQSVE 836
IR+ +++AGA +A+ SNHW EGGKGA+DLA+ +I +N DF FLY+V SVE
Sbjct: 779 IAAIRKAALEAGAFEAVTSNHWAEGGKGAIDLAKAVIEASNQP--VDFHFLYDV-NSSVE 835
Query: 837 DKISAIAKEMYGAGEVEFLPEAQAKIDLYTKQGFNNLPICIAKTQYSLSHDANLKGVPTG 896
DK++ I ++MYG ++ LPEAQ KID+Y +QGF NLPICIAKTQYSLSHDA LKGVPTG
Sbjct: 836 DKLTTIVQKMYGGAAIDILPEAQRKIDMYKEQGFGNLPICIAKTQYSLSHDATLKGVPTG 895
Query: 897 FKFPIRDVRASIGAGYLYALAAEIQTIPGLPTHCGFMNVEVNEDGEIDGMF 947
F FPIRDVR S GAGYLYALAAEIQTIPGL T+ G+M VEV++DGEIDG+F
Sbjct: 896 FTFPIRDVRLSNGAGYLYALAAEIQTIPGLATYAGYMAVEVDDDGEIDGLF 946
>YBR084w [H] KOG4230 C1-tetrahydrofolate synthase
Length = 975
Score = 1131 bits (2926), Expect = 0.0
Identities = 576/946 (60%), Positives = 714/946 (74%), Gaps = 8/946 (0%)
Query: 3 IIDGKAIANGIRDEIKDEISSLKEKYAVFRPTLTIFQVGNRPDSNTYVKMKLKAAKDAGI 62
I+ G+ +A IR++ DEI ++K K+ F+PTL I QVG RPDS+TYV+MKLKA+KD+ +
Sbjct: 37 ILSGRKLAQSIREKANDEIQAIKLKHPNFKPTLKIIQVGARPDSSTYVRMKLKASKDSNV 96
Query: 63 DVKFIHLDETVTEVELLGQIDDENSDDSVHGILVQLPLPAHLNEDKITSRVSACKDVDGF 122
D L +TEVELL +I D N DDS+HG+L+QLPLP HL+E IT+ V KDVDGF
Sbjct: 97 DCIIEKLPAEITEVELLKKISDINDDDSIHGLLIQLPLPRHLDETTITNAVDFKKDVDGF 156
Query: 123 GPVNIGELNKRNGTPYFQPCTPRGIIELLKRSGVTIAGSEAVVIGRSDIVGSPVATLLTS 182
N GEL K+ G PYF PCTP G ++LL+ + V + G AVV+GRS IVG+P+A+LL +
Sbjct: 157 HRYNAGELAKKGGKPYFIPCTPYGCMKLLEEAHVKLDGKNAVVLGRSSIVGNPIASLLKN 216
Query: 183 LDATVTILHSKSKDLREFISRADIVVVAIGQPEFIRGEWFANNKRGAVVIDVGTNFVKDE 242
+ATVT+ HS ++++ E +S+ADIV+ A G P++++ +W K GAVVIDVG N+V D
Sbjct: 217 ANATVTVCHSHTRNIAEVVSQADIVIAACGIPQYVKSDWI---KEGAVVIDVGINYVPDI 273
Query: 243 TRKSGYRNVGDVEFAAAEPYVKLITPVPGGVGPMTVAMLMQNTFESAKRYLDKQLNSGND 302
++KSG + VGDV+F + + ITPVPGGVGPMTVAML+ N +AKR +
Sbjct: 274 SKKSGQKLVGDVDFDSVKEKTSYITPVPGGVGPMTVAMLVSNVLLAAKRQFVES-EKLPV 332
Query: 303 FEPLKLNLLKPVPSDFEISRAQVPKPITQIAAESGILPSELEPYGDTKAKVKLDLLNRLK 362
+PL L+L PVPSD +ISRAQ PK I Q+A E GI ELE YG KAK+ ++ RL+
Sbjct: 333 IKPLPLHLESPVPSDIDISRAQSPKHIKQVAEELGIHSHELELYGHYKAKISPNIFKRLE 392
Query: 363 NRENGKYILVTGITPTPLGEGKSTTTVGLAQALGAHLKKKVFANVRQPSMGPTFGIKXXX 422
+RENGKY+LV GITPTPLGEGKSTTT+GL QAL AHL K ANVRQPS+GPT G+K
Sbjct: 393 SRENGKYVLVAGITPTPLGEGKSTTTMGLVQALSAHLGKPSIANVRQPSLGPTLGVKGGA 452
Query: 423 XXXXYSQVIPMDEFNLHVTGDIHAISMANNLLAAAIDTRMFHESTQSN-AALYKRLVPAK 481
Y+QVIPMDEFNLH+TGDIHAIS ANNLLAAAIDTRMFHE+TQ N + YKRLVP K
Sbjct: 453 AGGGYAQVIPMDEFNLHLTGDIHAISAANNLLAAAIDTRMFHEATQKNDSTFYKRLVPRK 512
Query: 482 KGVRKFTPTMLRRLKKLGINKTDPNELSAEESARFARLDIDPETITWRRVVDCNDRFLRG 541
KG+RKFTP+M RRLK+L I K DP+ L+ EE RFARL+I+P+TIT RRVVD NDR LR
Sbjct: 513 KGIRKFTPSMQRRLKRLDIEKEDPDALTPEEVKRFARLNINPDTITIRRVVDINDRMLRQ 572
Query: 542 ITVGQAPTERGHTRYTGFDISVASECMAVLALANSLEDMRERLGRIVIAASKSGEPITCE 601
IT+G+A TE+G TR TGFDI+VASE MA+LAL+ SL +M+ER+GR+VI A +P+T E
Sbjct: 573 ITIGEAATEKGFTRTTGFDITVASELMAILALSKSLHEMKERIGRMVIGADYDNKPVTVE 632
Query: 602 DIGCAGAMTALLKDAIKPNIMQTLEGTPVFVHAGPFANISIGANSVLADKMALKLAGVPT 661
DIGC GA+TALL+DAIKPN+MQTLEGTPV VHAGPFANISIGA+SV+AD MALKL G
Sbjct: 633 DIGCTGALTALLRDAIKPNLMQTLEGTPVMVHAGPFANISIGASSVIADLMALKLVGSEK 692
Query: 662 DLPEDVKKTQKGYVVTEAGFDFTMGGERFINIKCRSSGLVPDVVVIVATVRALKVHGGGP 721
+ D + GYVVTEAGFDF MGGERF +IKCRSSGLVPD VV+VATVRALK HGG P
Sbjct: 693 NPLNDKNIHEPGYVVTEAGFDFAMGGERFFDIKCRSSGLVPDAVVLVATVRALKSHGGAP 752
Query: 722 EVKAGAPLPSEYLNEDVELLRKGCANLAKHISNARAYNLPVVVAINKMSSDTDNEHEVIR 781
VK G LP EY E+++ + KG +NL K I N + + +PVVVAIN+ +D+ E EVI+
Sbjct: 753 NVKPGQSLPKEYTEENIDFVAKGVSNLVKQIENIKTFGIPVVVAINRFETDSQAEIEVIK 812
Query: 782 EESIKAGAVDAIVSNHWEEGGKGAVDLAEGIINTANNQSNEDFKFLYEVEGQSVEDKISA 841
+ ++ AGA A+ SNHW EGGKGAV+LA +++ N F FLY+V S+EDK+++
Sbjct: 813 KAALNAGASHAVTSNHWMEGGKGAVELAHAVVDATKEPKN--FNFLYDV-NSSIEDKLTS 869
Query: 842 IAKEMYGAGEVEFLPEAQAKIDLYTKQGFNNLPICIAKTQYSLSHDANLKGVPTGFKFPI 901
I ++MYG ++E PEAQ KID Y KQGF NLPICIAKTQYSLSHD +LKGVP GF FPI
Sbjct: 870 IVQKMYGGAKIEVSPEAQKKIDTYKKQGFGNLPICIAKTQYSLSHDPSLKGVPRGFTFPI 929
Query: 902 RDVRASIGAGYLYALAAEIQTIPGLPTHCGFMNVEVNEDGEIDGMF 947
RDVRASIGAGYLYALAAEIQTIPGL T+ G+M VEV++DGEI+G+F
Sbjct: 930 RDVRASIGAGYLYALAAEIQTIPGLSTYAGYMAVEVDDDGEIEGLF 975
>SPBC839.16 [H] KOG4230 C1-tetrahydrofolate synthase
Length = 937
Score = 1097 bits (2837), Expect = 0.0
Identities = 560/944 (59%), Positives = 699/944 (73%), Gaps = 11/944 (1%)
Query: 3 IIDGKAIANGIRDEIKDEISSLKEKYAVFRPTLTIFQVGNRPDSNTYVKMKLKAAKDAGI 62
+++G ++A +R+E++++ISS+K F +L I QVG R DSN YV+MK +AA +AGI
Sbjct: 4 LLEGTSLARKVREELREQISSIKSVDPYFNVSLKIIQVGGREDSNVYVRMKTRAANEAGI 63
Query: 63 DVKFIHLDETVTEVELLGQIDDENSDDSVHGILVQLPLPAHLNEDKITSRVSACKDVDGF 122
+ ++ E +TE +LL I N D +VHGI+VQLPLPAH+NE IT V+ KDVDGF
Sbjct: 64 SCEHVNFPEDITEYDLLLAIKGFNEDPTVHGIIVQLPLPAHINEQIITEAVAPEKDVDGF 123
Query: 123 GPVNIGELNKRNGTPYFQPCTPRGIIELLKRSGVTIAGSEAVVIGRSDIVGSPVATLLTS 182
N+G+L KR G P F CTP+GI+ +LK G+ + G AVVIGRS+IVG P++ LL
Sbjct: 124 CETNLGKLTKREGQPLFTACTPKGIMCILKHYGINVQGKHAVVIGRSNIVGRPMSILLEK 183
Query: 183 LDATVTILHSKSKDLREFISRADIVVVAIGQPEFIRGEWFANNKRGAVVIDVGTNFVKDE 242
+ATVT+ HSK++ + + + ADIVV AIG P F++ +W K+G V IDVG N + D
Sbjct: 184 ANATVTLCHSKTESIADIVRTADIVVAAIGIPHFVKADWL---KKGVVAIDVGINSIPDA 240
Query: 243 TRKSGYRNVGDVEFAAAEPYVKLITPVPGGVGPMTVAMLMQNTFESAKRYLDKQLNSGND 302
T+KSGYR GD++F A+ ITPVPG VGPMTVAML+QN ESA R+
Sbjct: 241 TKKSGYRLTGDIDFENAKEVASAITPVPGSVGPMTVAMLLQNVVESAVRFRKMSRKRKPT 300
Query: 303 FEPLKLNLLKPVPSDFEISRAQVPKPITQIAAESGILPSELEPYGDTKAKVKLDLLNRLK 362
PLKL PVPSD EI+R+Q PK I +A+E GI SELE YG KAKV L++L RL
Sbjct: 301 LLPLKLQT--PVPSDIEIARSQTPKNIGDLASEIGIAKSELEFYGSHKAKVNLEILQRLA 358
Query: 363 NRENGKYILVTGITPTPLGEGKSTTTVGLAQALGAHLKKKVFANVRQPSMGPTFGIKXXX 422
+R +G Y++VTGITPTP GEGKST T GL QAL ++L K A VRQPS GPTFGIK
Sbjct: 359 HRRDGHYVVVTGITPTPFGEGKSTLTAGLVQAL-SNLDKLAIACVRQPSQGPTFGIKGGA 417
Query: 423 XXXXYSQVIPMDEFNLHVTGDIHAISMANNLLAAAIDTRMFHESTQSNAALYKRLVPAKK 482
YSQ IPM+EFNLH+TGDIHAI+ A NLLAAAIDTRMFHE+TQS+AALYKRL K
Sbjct: 418 AGGGYSQFIPMEEFNLHLTGDIHAITAATNLLAAAIDTRMFHENTQSDAALYKRLTLVKG 477
Query: 483 GVRKFTPTMLRRLKKLGINKTDPNELSAEESARFARLDIDPETITWRRVVDCNDRFLRGI 542
R+F P M RRLKKLGI+KT+P EL+ EE +FARLDI+P TI+W R +D NDRFLR I
Sbjct: 478 NKREFAPVMFRRLKKLGIDKTNPEELTEEEQRKFARLDIEPSTISWNRTLDVNDRFLRKI 537
Query: 543 TVGQAPTERGHTRYTGFDISVASECMAVLALANSLEDMRERLGRIVIAASKSGEPITCED 602
T+G+ PTE+G TR TGFD+SVASECM+VLALA L+DMRERLGR+V+A++KSGEP+T +D
Sbjct: 538 TIGENPTEKGFTRQTGFDLSVASECMSVLALATDLKDMRERLGRMVVASNKSGEPVTADD 597
Query: 603 IGCAGAMTALLKDAIKPNIMQTLEGTPVFVHAGPFANISIGANSVLADKMALKLAGVPTD 662
+G GA+T LLKDAIKP +MQTLEGTP VHAGPFANISIGA+S+LAD++ALKLAG T+
Sbjct: 598 LGVGGALTVLLKDAIKPTLMQTLEGTPALVHAGPFANISIGASSILADRIALKLAG--TE 655
Query: 663 LPEDVKKTQKGYVVTEAGFDFTMGGERFINIKCRSSGLVPDVVVIVATVRALKVHGGGPE 722
+ ED KK + GYVVTEAGF +G E+F NIKCR+SGL PD +VIVATV+ALK+HGGGP
Sbjct: 656 VDEDAKK-EAGYVVTEAGFASDIGMEKFFNIKCRTSGLKPDAIVIVATVQALKLHGGGPP 714
Query: 723 VKAGAPLPSEYLNEDVELLRKGCANLAKHISNARAYNLPVVVAINKMSSDTDNEHEVIRE 782
V G P+P Y EDV+L+RKGCANLAKHISNAR Y LPVVVAINK SSD+ NE IRE
Sbjct: 715 VGPGKPIPEVYKREDVDLVRKGCANLAKHISNARKYGLPVVVAINKFSSDSPNEISAIRE 774
Query: 783 ESIKAGAVDAIVSNHWEEGGKGAVDLAEGIINTANNQSNEDFKFLYEVEGQSVEDKISAI 842
E++ AGA DA+ SNHW EGGKGA+ +A +IN N +E F+ LY+V + +E KI I
Sbjct: 775 EALAAGATDAVDSNHWAEGGKGALGVARALINACENVDSE-FRLLYDVH-EPIEKKIEII 832
Query: 843 AKEMYGAGEVEFLPEAQAKIDLYTKQGFNNLPICIAKTQYSLSHDANLKGVPTGFKFPIR 902
AKEMYGA +E P A+ +++ +TKQG+NNLPICIAKTQYSLSHD +LKG PT F PIR
Sbjct: 833 AKEMYGADGIELSPLAKERLETFTKQGYNNLPICIAKTQYSLSHDPDLKGAPTNFTVPIR 892
Query: 903 DVRASIGAGYLYALAAEIQTIPGLPTHCGFMNVEVNEDGEIDGM 946
D+R S GAG++Y LAA I TIPGLPT + N+++ E+G+I G+
Sbjct: 893 DMRLSAGAGFIYPLAAAISTIPGLPTKPAYYNIDIAENGDIVGL 936
>Hs13699868 [H] KOG4230 C1-tetrahydrofolate synthase
Length = 935
Score = 1043 bits (2696), Expect = 0.0
Identities = 542/948 (57%), Positives = 695/948 (73%), Gaps = 21/948 (2%)
Query: 3 IIDGKAIANGIRDEIKDEISSLKEKYAVFRPTLTIFQVGNRPDSNTYVKMKLKAAKDAGI 62
I++GK I+ IR +K++++ LKE+ F P L I QVGNR DSN Y+ +KLKAA++ GI
Sbjct: 6 ILNGKEISAQIRARLKNQVTQLKEQVPGFTPRLAILQVGNRDDSNLYINVKLKAAEEIGI 65
Query: 63 DVKFIHLDETVTEVELLGQIDDENSDDSVHGILVQLPLPAH--LNEDKITSRVSACKDVD 120
I L T TE E++ I N D +VHG LVQLPL + +N +++ + ++ KDVD
Sbjct: 66 KATHIKLPRTTTESEVMKYITSLNEDSTVHGFLVQLPLDSENSINTEEVINAIAPEKDVD 125
Query: 121 GFGPVNIGELNKRNGTPYFQPCTPRGIIELLKRSGVTIAGSEAVVIGRSDIVGSPVATLL 180
G +N G L + + F PCTP+G +EL+K +GV IAG AVV+GRS IVG+P+ LL
Sbjct: 126 GLTSINAGRLARGDLNDCFIPCTPKGCLELIKETGVPIAGRHAVVVGRSKIVGAPMHDLL 185
Query: 181 TSLDATVTILHSKSKDLREFISRADIVVVAIGQPEFIRGEWFANNKRGAVVIDVGTNFVK 240
+ATVT HSK+ L E +++ DI+VVA GQPE ++GEW K GA+VID G N+V
Sbjct: 186 LWNNATVTTCHSKTAHLDEEVNKGDILVVATGQPEMVKGEWI---KPGAIVIDCGINYVP 242
Query: 241 DETRKSGYRNVGDVEFAAAEPYVKLITPVPGGVGPMTVAMLMQNTFESAKRYLDKQLNSG 300
D+ + +G + VGDV + A+ ITPVPGGVGPMTVAMLMQ+T ESAKR+L+K
Sbjct: 243 DDKKPNGRKVVGDVAYDEAKERASFITPVPGGVGPMTVAMLMQSTVESAKRFLEKFKPGK 302
Query: 301 NDFEPLKLNLLKPVPSDFEISRAQVPKPITQIAAESGILPSELEPYGDTKAKVKLDLLNR 360
+ LNL PVPSD +ISR+ PKPI ++A E G+L E+E YG+TKAKV L L R
Sbjct: 303 WMIQYNNLNLKTPVPSDIDISRSCKPKPIGKLAREIGLLSEEVELYGETKAKVLLSALER 362
Query: 361 LKNRENGKYILVTGITPTPLGEGKSTTTVGLAQALGAHLKKKVFANVRQPSMGPTFGIKX 420
LK+R +GKY++VTGITPTPLGEGKSTTT+GL QALGAHL + VFA VRQPS GPTFGIK
Sbjct: 363 LKHRPDGKYVVVTGITPTPLGEGKSTTTIGLVQALGAHLYQNVFACVRQPSQGPTFGIKG 422
Query: 421 XXXXXXYSQVIPMDEFNLHVTGDIHAISMANNLLAAAIDTRMFHESTQSNAALYKRLVPA 480
YSQVIPM+EFNLH+TGDIHAI+ ANNL+AAAID R+FHE TQ++ AL+ RLVP+
Sbjct: 423 GAAGGGYSQVIPMEEFNLHLTGDIHAITAANNLVAAAIDARIFHELTQTDKALFNRLVPS 482
Query: 481 KKGVRKFTPTMLRRLKKLGINKTDPNELSAEESARFARLDIDPETITWRRVVDCNDRFLR 540
GVR+F+ +RRLK+LGI KTDP L+ EE RFARLDIDPETITW+RV+D NDRFLR
Sbjct: 483 VNGVRRFSDIQIRRLKRLGIEKTDPTTLTDEEINRFARLDIDPETITWQRVLDTNDRFLR 542
Query: 541 GITVGQAPTERGHTRYTGFDISVASECMAVLALANSLEDMRERLGRIVIAASKSGEPITC 600
IT+GQAPTE+GHTR FDISVASE MAVLAL SLEDMRERLG++V+A+SK GEP++
Sbjct: 543 KITIGQAPTEKGHTRTAQFDISVASEIMAVLALTTSLEDMRERLGKMVVASSKKGEPVSA 602
Query: 601 EDIGCAGAMTALLKDAIKPNIMQTLEGTPVFVHAGPFANISIGANSVLADKMALKLAGVP 660
ED+G +GA+T L+KDAIKPN+MQTLEGTPVFVHAGPFANI+ G +S++AD++ALKL G
Sbjct: 603 EDLGVSGALTVLMKDAIKPNLMQTLEGTPVFVHAGPFANIAHGNSSIIADQIALKLVG-- 660
Query: 661 TDLPEDVKKTQKGYVVTEAGFDFTMGGERFINIKCRSSGLVPDVVVIVATVRALKVHGGG 720
PE G+VVTEAGF +G E+F NIKCR SGL P VVV+VATVRALK+HGGG
Sbjct: 661 ---PE-------GFVVTEAGFGADIGMEKFFNIKCRYSGLCPHVVVLVATVRALKMHGGG 710
Query: 721 PEVKAGAPLPSEYLNEDVELLRKGCANLAKHISNARAYNLPVVVAINKMSSDTDNEHEVI 780
P V AG PLP Y+ E++EL+ KG +NL K I NAR + +PVVVA+N +DT++E ++I
Sbjct: 711 PTVTAGLPLPKAYIQENLELVEKGFSNLKKQIENARMFGIPVVVAVNAFKTDTESELDLI 770
Query: 781 REESIKAGAVDAIVSNHWEEGGKGAVDLAEGIINTANNQSNEDFKFLYEVEGQSVEDKIS 840
S + GA DA+ HW EGGKGA+ LA+ + A Q+ F+ LY+++ VEDKI
Sbjct: 771 SRLSREHGAFDAVKCTHWAEGGKGALALAQAVQRAA--QAPSSFQLLYDLK-LPVEDKIR 827
Query: 841 AIAKEMYGAGEVEFLPEAQAKIDLYTKQGFNNLPICIAKTQYSLSHDANLKGVPTGFKFP 900
IA+++YGA ++E LPEAQ K ++YTKQGF NLPIC+AKT SLSH+ KGVPTGF P
Sbjct: 828 IIAQKIYGADDIELLPEAQHKAEVYTKQGFGNLPICMAKTHLSLSHNPEQKGVPTGFILP 887
Query: 901 IRDVRASIGAGYLYALAAEIQTIPGLPTHCGFMNVEVN-EDGEIDGMF 947
IRD+RAS+GAG+LY L + T+PGLPT F +++++ E +++G+F
Sbjct: 888 IRDIRASVGAGFLYPLVGTMSTMPGLPTRPCFYDIDLDPETEQVNGLF 935
>SPBC2G2.08 [H] KOG4230 C1-tetrahydrofolate synthase
Length = 969
Score = 1016 bits (2628), Expect = 0.0
Identities = 524/950 (55%), Positives = 681/950 (71%), Gaps = 22/950 (2%)
Query: 3 IIDGKAIANGIRDEIKDEISSLKEKYAVFRPTLTIFQVGNRPDSNTYVKMKLKAAKDAGI 62
I++GK +A ++ + +E+ +LK K F+P L I QVG R DSN YV+MK KAA+ GI
Sbjct: 35 ILNGKLLARKLQRSVAEEVQALKAKDRNFKPALAIVQVGKREDSNVYVRMKEKAARLVGI 94
Query: 63 DVKFIHLDETVTEVELLGQIDDENSDDSVHGILVQLPLPAHLNEDKITSRVSACKDVDGF 122
D K+ ET+ LL ++ N D +VHG+LVQLPLP HLNE +T ++ KDVDGF
Sbjct: 95 DFKYCPFPETIQMPALLHELKKLNDDHTVHGVLVQLPLPKHLNERTVTESITPPKDVDGF 154
Query: 123 GPVNIGELNKRNGTPYFQPCTPRGIIELLKRSGVTIAGSEAVVIGRSDIVGSPVATLLTS 182
G NIG L K + TP PCTP+GI+ELLK + +++AG AVV+GRSDIVG+P++ LL
Sbjct: 155 GAFNIGLLAKNDATPIHYPCTPKGIMELLKDNKISVAGLNAVVLGRSDIVGNPISYLLRK 214
Query: 183 LDATVTILHSKSKDLREFISRADIVVVAIGQPEFIRGEWFANNKRGAVVIDVGTNFVKDE 242
+ATVT+ HSK+KDL + IS AD+V+ A+G+PEF+RGEW K G+VV+DVG N V
Sbjct: 215 DNATVTVCHSKTKDLIQHISNADLVIAALGKPEFVRGEWL---KPGSVVVDVGINAV--- 268
Query: 243 TRKSGYR-NVGDVEFAAAEPYVKLITPVPGGVGPMTVAMLMQNTFESAKRYLDKQLNSGN 301
+++G R VGDV F +A ITPVPGGVGPMTVAMLM+N +AK + +
Sbjct: 269 -QRNGKRVLVGDVHFESASKVASSITPVPGGVGPMTVAMLMENIVNAAKIARTENIYRKI 327
Query: 302 DFEPLKLNLLKPVPSDFEISRAQVPKPITQIAAESGILPSELEPYGDTKAKVKLDLLNRL 361
D PL+L KPVPSD EI+ +Q PK I+ +A E GI +ELE YG+ KAKV L + RL
Sbjct: 328 DLNPLELK--KPVPSDIEIANSQEPKLISNLAKEMGIYDTELENYGNYKAKVNLAVYERL 385
Query: 362 KNRENGKYILVTGITPTPLGEGKSTTTVGLAQALGAHLKKKVFANVRQPSMGPTFGIKXX 421
K+R++G Y++V+GITPTP GEGKST GL QA+G HL K A VRQPS GPTFG+K
Sbjct: 386 KHRKDGNYVVVSGITPTPFGEGKSTVVAGLVQAMG-HLGKLGIACVRQPSQGPTFGVKGG 444
Query: 422 XXXXXYSQVIPMDEFNLHVTGDIHAISMANNLLAAAIDTRMFHESTQSNAALYKRLVPAK 481
Y+Q IPMD+FNLH+TGDIHA++ ANNLL AA++TRMFHE+TQS+AAL KRL+P K
Sbjct: 445 AAGGGYAQFIPMDDFNLHMTGDIHAVTAANNLLVAALETRMFHENTQSDAALIKRLIPVK 504
Query: 482 KGVRKFTPTMLRRLKKL----GINKTDPNELSAEESARFARLDIDPETITWRRVVDCNDR 537
G R ++ R ++ ++ D N S E F RL++DP+TI RV+D NDR
Sbjct: 505 NGRRVIPRGLIGRWNRICASHNMDPEDVNNASPELLKEFVRLNVDPDTIECNRVLDVNDR 564
Query: 538 FLRGITVGQAPTERGHTRYTGFDISVASECMAVLALANSLEDMRERLGRIVIAASKSGEP 597
FLR I VG+A TE+GH R T FDISVASECM++LAL+ L DM RL R+VIA K G
Sbjct: 565 FLRSIEVGKASTEKGHVRKTSFDISVASECMSILALSCDLNDMHSRLSRMVIANDKYGNA 624
Query: 598 ITCEDIGCAGAMTALLKDAIKPNIMQTLEGTPVFVHAGPFANISIGANSVLADKMALKLA 657
IT D+G +GA+T LLKDAIKPN+MQTLEGTP FVHAGPFANISIGA+S++ADK+ALKLA
Sbjct: 625 ITAGDLGVSGALTVLLKDAIKPNLMQTLEGTPAFVHAGPFANISIGASSIIADKIALKLA 684
Query: 658 GVPT-DLPEDVKKTQKGYVVTEAGFDFTMGGERFINIKCRSSGLVPDVVVIVATVRALKV 716
G + D PED GYVVTEAGF MG E+F NIKCR S LVP+ VV+V TV+ALK+
Sbjct: 685 GTESFDRPEDA-----GYVVTEAGFASDMGMEKFFNIKCRYSKLVPNTVVLVTTVKALKL 739
Query: 717 HGGGPEVKAGAPLPSEYLNEDVELLRKGCANLAKHISNARAYNLPVVVAINKMSSDTDNE 776
HGGGP++K GAP+P EYL E+++L++ GC+N+ KHI N +N+PVVVAIN +D+ E
Sbjct: 740 HGGGPKLKPGAPIPEEYLVENLDLVKNGCSNMVKHIQNCHKFNIPVVVAINSYKTDSSKE 799
Query: 777 HEVIREESIKAGAVDAIVSNHWEEGGKGAVDLAEGIINTANNQSNEDFKFLYEVEGQSVE 836
HE+IRE +++AGAVDA+ S+HW +GGKGA++LA+ ++ + SN F+ LY+ E S+E
Sbjct: 800 HEIIREAALQAGAVDAVPSDHWAQGGKGAIELAKSVMTACDQSSNSKFRLLYDSE-TSIE 858
Query: 837 DKISAIAKEMYGAGEVEFLPEAQAKIDLYTKQGFNNLPICIAKTQYSLSHDANLKGVPTG 896
DK++ IAKEMYGA VEF A+ +I+ + KQGF NLPIC+AKTQYSLSH+ + VP
Sbjct: 859 DKVNVIAKEMYGANGVEFSSLAKERINTFIKQGFGNLPICMAKTQYSLSHNPEFRNVPKN 918
Query: 897 FKFPIRDVRASIGAGYLYALAAEIQTIPGLPTHCGFMNVEVNEDGEIDGM 946
F PIRD+R + GAG++Y LAAEIQTIPGLPT ++N+++ E+GEI G+
Sbjct: 919 FTVPIRDMRLNAGAGFIYPLAAEIQTIPGLPTAPAYLNIDICENGEIVGL 968
>Hs22055576 [H] KOG4230 C1-tetrahydrofolate synthase
Length = 755
Score = 503 bits (1295), Expect = e-142
Identities = 347/914 (37%), Positives = 473/914 (50%), Gaps = 183/914 (20%)
Query: 3 IIDGKAIANGIRDEIKDEISSLKEKYAVFRPTLTIFQVGNRPDSNTYVKMKLKAAKDAGI 62
I++ K I+ IR +K++++ LK+KY LKAA++ G+
Sbjct: 6 ILNRKEISAQIRARLKNQVTQLKDKYL------------------------LKAAEEIGM 41
Query: 63 DVKFIHLDETVTEVELLGQIDDENSDDSVHGILVQLPLPAHLNEDKITSRVSACKDVDGF 122
I L T TE E++ I N D +VH + + + ++ KDVDG
Sbjct: 42 KATDIKLPRTTTESEVIKYIISLNEDSTVH----------RQYTEGVINAIAPEKDVDGL 91
Query: 123 GPVNIGELNKRNGTPYFQPCTPRGIIELLKRSGVTIAGSEAVVIGRSDIVGSPVATLLTS 182
++ G+L + + F PC P+G +EL+K +GV IA AVV+G S IV + + LL
Sbjct: 92 TSISAGKLARGDLNDCFIPCMPKGCLELMKETGVQIARRHAVVVGCSKIVRALMRDLLLW 151
Query: 183 LDATVTILHSKSKDLREFISRADIVVVAIGQPEFIRGEWFANNKRGAVVIDVGTNFVKDE 242
+ATVT HSK+ +L E N A+VI+ G N+V D+
Sbjct: 152 NNATVTTCHSKTANLDEE----------------------GNGSNLAIVINCGINYVPDD 189
Query: 243 TRKSGYRNVGDVEFAAAEPYVKLITPVPGGVGPMTVAMLMQNTFESAKRYLDKQLNSGND 302
+ ++ MTVAMLMQ+T ESAKR+L+K
Sbjct: 190 KKNKWEKS-----------------------WLMTVAMLMQSTVESAKRFLEKFKPGKWM 226
Query: 303 FEPLKLNLLKPVPSDFEISRAQVPKPITQIAAESGILPSELEPYGDTKAKVKLDLLNRLK 362
+ LNL PVPSD +ISR+ PKPI E+E YG+TKAKV L L RLK
Sbjct: 227 IQYNNLNLKTPVPSDIDISRSCKPKPI------------EVELYGETKAKVLLSALERLK 274
Query: 363 NRENGKYILVTGITPTPLGEGKSTTTVGLAQALGAHLKKKVFANVRQPSMGPTFGIKXXX 422
++ GKY +VTGITPTPLGEGKSTTT+GL QAL AHL + F VRQPS GPTFGIK
Sbjct: 275 HQPVGKYQVVTGITPTPLGEGKSTTTIGLVQALLAHLYQNFFMCVRQPSQGPTFGIKGGV 334
Query: 423 XXXXYSQVIPMDEFNLHVTGDIHAISMANNLLAAAIDTRMFHESTQSNAALYKRLVPAKK 482
YSQVIPM+EFNLH+TGDIHAI+++NNL+AAAID ++FHE TQ++ A + LVP+
Sbjct: 335 AGGGYSQVIPMEEFNLHLTGDIHAITVSNNLVAAAIDAQIFHELTQTDKATFTHLVPSVN 394
Query: 483 GVRKFTPTMLRRLKKLGINKTDPNELSAEESARFARLDIDPETITWRRVVDCNDRFLRGI 542
GVRKF+ ++RL N L +++ D T+T + +RF R
Sbjct: 395 GVRKFSDIHIQRL----------NRLGIDKT--------DSTTLTDEEI----NRFAR-- 430
Query: 543 TVGQAPTERGHTRYTGFDISVASECMAVLALANSLEDMRERLGRIVIAASKSGEPITCED 602
+ + E +A + N+ + ++ KS D
Sbjct: 431 ------------------LDIDPETIAWQRVLNTNDRFLRKITIGQAPTKKSHTQTVHFD 472
Query: 603 IGCAGAMTALLKDAIKPNIMQTLEGTPVFVHAGPFANISIGANSVLADKMALKLAGVPTD 662
I A + A+L A+ ++ E V A + A L L G
Sbjct: 473 ISVASEIMAVL--ALTTSLEDRRERLGKMVVASSKKGDPVSAED-------LGLVG---- 519
Query: 663 LPEDVKKTQKGYVVTEAGFDFTMGGERFINIKCRSSGLVPDVVVIVATVRALKVHGGGPE 722
PE G+VVTEAGF +G E+F+NIK + SGL P VVV+VATV ALK+H GGP
Sbjct: 520 -PE-------GFVVTEAGFGADIGIEKFLNIKSQYSGLHPHVVVLVATVSALKMHRGGPM 571
Query: 723 VKAGAPLPSEYLNEDVELLRKGCANLAKHISNARAYNLPVVVAINKMSSDTDNEHEVIRE 782
V AG PLP Y+ E++EL+ KG +NL K NAR + +PVVVA+N +DT+ E ++I
Sbjct: 572 VTAGMPLPKAYIEENLELVEKGFSNLKKQTENARMFGIPVVVAVNAFKTDTETELDLIGC 631
Query: 783 ESIKAGAVDAIVSNHWEEGGKGAVDLAEGIINTANNQSNEDFKFLYEVEGQSVEDKISAI 842
S + A DA+ HW++G + VEDKI I
Sbjct: 632 LSREQRAFDAVKCTHWQKGAR-----------------------------LPVEDKIRII 662
Query: 843 AKEMYGAGEVEFLPEAQAKIDLYTKQGFNNLPICIAKTQYSLSHDANLKGVPTGFKFPIR 902
A+++YGA +E L +AQ K ++YTKQGF NLPIC+AKT SLSH+ KGVPTGF PIR
Sbjct: 663 AQKIYGADNIELLLKAQHKAEVYTKQGFGNLPICMAKTHLSLSHNPERKGVPTGFILPIR 722
Query: 903 DVRASIGAGYLYAL 916
++ ASIGAG+LY L
Sbjct: 723 NIHASIGAGFLYPL 736
>At3g12290 [H] KOG0089 Methylenetetrahydrofolate
dehydrogenase/methylenetetrahydrofolate cyclohydrolase
Length = 299
Score = 298 bits (762), Expect = 3e-80
Identities = 159/289 (55%), Positives = 195/289 (67%), Gaps = 4/289 (1%)
Query: 3 IIDGKAIANGIRDEIKDEISSLKEKYAVFRPTLTIFQVGNRPDSNTYVKMKLKAAKDAGI 62
IIDGKAIA+ IR EI +E+ L EK+ P L + VG+R DS TYV K KA + GI
Sbjct: 11 IIDGKAIAHTIRSEIAEEVRGLSEKHGKV-PGLAVVIVGSRKDSQTYVNTKRKACAEVGI 69
Query: 63 DVKFIHLDETVTEVELLGQIDDENSDDSVHGILVQLPLPAHLNEDKITSRVSACKDVDGF 122
+ L E V+E +L+ ++ + NS+ VHGILVQLPLP H+NE+ I +S KDVDGF
Sbjct: 70 KSFDVGLPEEVSEADLISKVHELNSNPDVHGILVQLPLPKHINEEHILGAISIDKDVDGF 129
Query: 123 GPVNIGELNKRNGTPYFQPCTPRGIIELLKRSGVTIAGSEAVVIGRSDIVGSPVATLLTS 182
P+NIG+L + P F PCTP+G +ELL RSGV I G AVV+GRS+IVG PV+ LL
Sbjct: 130 HPLNIGKLAMKGREPLFLPCTPKGCLELLARSGVKIKGQRAVVVGRSNIVGLPVSLLLLK 189
Query: 183 LDATVTILHSKSKDLREFISRADIVVVAIGQPEFIRGEWFANNKRGAVVIDVGTNFVKDE 242
DATVT +HS +KD I ADIV+ A GQ I+G W K GA VIDVGTN V D
Sbjct: 190 ADATVTTVHSHTKDPEAIIREADIVIAACGQAHMIKGNWI---KPGAAVIDVGTNAVSDP 246
Query: 243 TRKSGYRNVGDVEFAAAEPYVKLITPVPGGVGPMTVAMLMQNTFESAKR 291
++KSGYR VGDV+FA A ITPVPGGVGPMTVAML++NT + AKR
Sbjct: 247 SKKSGYRLVGDVDFAEASKVAGFITPVPGGVGPMTVAMLLRNTVDGAKR 295
>At4g00620 [H] KOG0089 Methylenetetrahydrofolate
dehydrogenase/methylenetetrahydrofolate cyclohydrolase
Length = 360
Score = 283 bits (724), Expect = 9e-76
Identities = 153/289 (52%), Positives = 188/289 (64%), Gaps = 4/289 (1%)
Query: 3 IIDGKAIANGIRDEIKDEISSLKEKYAVFRPTLTIFQVGNRPDSNTYVKMKLKAAKDAGI 62
+IDGKA+A IRDEI E+S +KE V P L + VG+R DS TYV+ K KA GI
Sbjct: 71 VIDGKAVAKKIRDEITIEVSRMKESIGVI-PGLAVILVGDRKDSATYVRNKKKACDSVGI 129
Query: 63 DVKFIHLDETVTEVELLGQIDDENSDDSVHGILVQLPLPAHLNEDKITSRVSACKDVDGF 122
+ L E +E E+L + N D SVHGILVQLPLP+H++E I + VS KDVDGF
Sbjct: 130 KSFEVRLAEDSSEEEVLKSVSGFNDDPSVHGILVQLPLPSHMDEQNILNAVSIEKDVDGF 189
Query: 123 GPVNIGELNKRNGTPYFQPCTPRGIIELLKRSGVTIAGSEAVVIGRSDIVGSPVATLLTS 182
P+NIG L R P F PCTP+G IELL R + I G AVVIGRS+IVG P A LL
Sbjct: 190 HPLNIGRLAMRGREPLFVPCTPKGCIELLHRYNIEIKGKRAVVIGRSNIVGMPAALLLQR 249
Query: 183 LDATVTILHSKSKDLREFISRADIVVVAIGQPEFIRGEWFANNKRGAVVIDVGTNFVKDE 242
DATV+I+HS++K+ E ADI++ A+GQP +RG W K GAV+IDVG N V+D
Sbjct: 250 EDATVSIIHSRTKNPEEITREADIIISAVGQPNMVRGSWI---KPGAVLIDVGINPVEDP 306
Query: 243 TRKSGYRNVGDVEFAAAEPYVKLITPVPGGVGPMTVAMLMQNTFESAKR 291
+ GYR VGD+ + A ITPVPGGVGPMT+AML+ NT SAKR
Sbjct: 307 SAARGYRLVGDICYEEASKVASAITPVPGGVGPMTIAMLLSNTLTSAKR 355
>At2g38660 [H] KOG0089 Methylenetetrahydrofolate
dehydrogenase/methylenetetrahydrofolate cyclohydrolase
Length = 350
Score = 263 bits (671), Expect = 1e-69
Identities = 144/289 (49%), Positives = 183/289 (62%), Gaps = 4/289 (1%)
Query: 3 IIDGKAIANGIRDEIKDEISSLKEKYAVFRPTLTIFQVGNRPDSNTYVKMKLKAAKDAGI 62
+IDG IA IR +I E+ +K+ P L + VG + DS TYV+ K+KA ++ GI
Sbjct: 63 VIDGNVIAEEIRTKIISEVGKMKKAVGKV-PGLAVVLVGEQRDSQTYVRNKIKACEETGI 121
Query: 63 DVKFIHLDETVTEVELLGQIDDENSDDSVHGILVQLPLPAHLNEDKITSRVSACKDVDGF 122
L E TE +++ + N D S+HGILVQLPLP HLNE KI + V KDVDGF
Sbjct: 122 KSVLAELPEDCTEGQIISVLRKFNEDTSIHGILVQLPLPQHLNESKILNMVRLEKDVDGF 181
Query: 123 GPVNIGELNKRNGTPYFQPCTPRGIIELLKRSGVTIAGSEAVVIGRSDIVGSPVATLLTS 182
P+N+G L R P F CTP+G +ELL R+GV IAG AVVIGRS+IVG P++ LL
Sbjct: 182 HPLNVGNLAMRGREPLFVSCTPKGCVELLIRTGVEIAGKNAVVIGRSNIVGLPMSLLLQR 241
Query: 183 LDATVTILHSKSKDLREFISRADIVVVAIGQPEFIRGEWFANNKRGAVVIDVGTNFVKDE 242
DATV+ +H+ +KD +ADIV+ A G P +RG W K GAVVIDVGT V+D
Sbjct: 242 HDATVSTVHAFTKDPEHITRKADIVIAAAGIPNLVRGSWL---KPGAVVIDVGTTPVEDS 298
Query: 243 TRKSGYRNVGDVEFAAAEPYVKLITPVPGGVGPMTVAMLMQNTFESAKR 291
+ + GYR VGDV + A ITPVPGGVGPMT+ ML+ NT E+AKR
Sbjct: 299 SCEFGYRLVGDVCYEEALGVASAITPVPGGVGPMTITMLLCNTLEAAKR 347
>Hs22053503 [H] KOG4230 C1-tetrahydrofolate synthase
Length = 218
Score = 245 bits (625), Expect = 3e-64
Identities = 125/209 (59%), Positives = 153/209 (72%)
Query: 288 SAKRYLDKQLNSGNDFEPLKLNLLKPVPSDFEISRAQVPKPITQIAAESGILPSELEPYG 347
S +R+L +Q + LKL L PVPSD EISR Q PK + +A E G+L E+E YG
Sbjct: 4 SGRRWLREQQHRRWRLHCLKLQPLSPVPSDIEISRGQTPKAVDVLAKEIGLLADEIEIYG 63
Query: 348 DTKAKVKLDLLNRLKNRENGKYILVTGITPTPLGEGKSTTTVGLAQALGAHLKKKVFANV 407
+KAKV+L +L RLK++ +GKY+LV GITPTPLGEGKST T+GL QAL AHL FA +
Sbjct: 64 KSKAKVRLSVLERLKDQADGKYVLVAGITPTPLGEGKSTVTIGLVQALTAHLNVNSFACL 123
Query: 408 RQPSMGPTFGIKXXXXXXXYSQVIPMDEFNLHVTGDIHAISMANNLLAAAIDTRMFHEST 467
RQPS GPTFG+K Y+QVIPM+EFNLH+TGDIHAI+ ANNLLAAAIDTR+ HE+T
Sbjct: 124 RQPSQGPTFGVKGGAAGGGYAQVIPMEEFNLHLTGDIHAITAANNLLAAAIDTRILHENT 183
Query: 468 QSNAALYKRLVPAKKGVRKFTPTMLRRLK 496
Q++ ALY RLVP GVR+F+ L RLK
Sbjct: 184 QTDKALYNRLVPLVNGVREFSEIQLARLK 212
>At4g00600 [H] KOG0089 Methylenetetrahydrofolate
dehydrogenase/methylenetetrahydrofolate cyclohydrolase
Length = 310
Score = 228 bits (580), Expect = 4e-59
Identities = 131/289 (45%), Positives = 167/289 (57%), Gaps = 40/289 (13%)
Query: 3 IIDGKAIANGIRDEIKDEISSLKEKYAVFRPTLTIFQVGNRPDSNTYVKMKLKAAKDAGI 62
+IDGKA A IRD+IK E+S +KE V A+D+
Sbjct: 57 VIDGKAEAKKIRDDIKIEVSRMKESIGVV------------------------PAEDS-- 90
Query: 63 DVKFIHLDETVTEVELLGQIDDENSDDSVHGILVQLPLPAHLNEDKITSRVSACKDVDGF 122
+E E+L + N D SVHG+LVQLPLP+H++E I + VS KDVDGF
Sbjct: 91 -----------SEEEVLKYVSGFNDDPSVHGVLVQLPLPSHMDEQNILNAVSIEKDVDGF 139
Query: 123 GPVNIGELNKRNGTPYFQPCTPRGIIELLKRSGVTIAGSEAVVIGRSDIVGSPVATLLTS 182
P+NIG L R P F PCTP+G IELL R + G AVVIGRS+IVG P A LL
Sbjct: 140 HPLNIGRLAMRGREPLFVPCTPKGCIELLHRYNIEFKGKRAVVIGRSNIVGMPAALLLQK 199
Query: 183 LDATVTILHSKSKDLREFISRADIVVVAIGQPEFIRGEWFANNKRGAVVIDVGTNFVKDE 242
DATV+I+HS++ + E +ADI++ A+G+P +RG W K GAV+IDVG V+D
Sbjct: 200 EDATVSIIHSRTMNPEELTRQADILISAVGKPNMVRGSWI---KPGAVLIDVGIKPVEDP 256
Query: 243 TRKSGYRNVGDVEFAAAEPYVKLITPVPGGVGPMTVAMLMQNTFESAKR 291
+ G R VGD+ + A ITPVPG VGPMT+AML+ NT SAKR
Sbjct: 257 SAAGGERLVGDICYVEASKIASAITPVPGDVGPMTIAMLLSNTLTSAKR 305
>CE25047 [H] KOG4230 C1-tetrahydrofolate synthase
Length = 303
Score = 215 bits (548), Expect = 2e-55
Identities = 123/294 (41%), Positives = 172/294 (57%), Gaps = 11/294 (3%)
Query: 3 IIDGKAIANGIRDEIKDEISSLKEKYAVFRPTLTIFQVGNRPDSNTYVKMKLKAAKDAGI 62
I+ G + + ++ +I+ +E + F L I QVGNR DSN Y+ KLK AK+ G
Sbjct: 5 IVSGLEYSKKVLHDVGQKIAKTREHHPNFHAVLAIVQVGNRSDSNVYINSKLKKAKEIGA 64
Query: 63 DVKFIHLDETVTEVELLGQIDDENSDDSVHGILVQLPLPA--HLNEDKITSRVSACKDVD 120
D K I L +T+T+ +L +I N D+ + GI++QLPL ++ D + + KDVD
Sbjct: 65 DGKLIKLPDTITQGDLKREIMALNHDNEIDGIIIQLPLDCKHEIDADSVIDLIDPLKDVD 124
Query: 121 GFGPVNIGELNKRNGTPYFQPCTPRGIIELLKRS---GVTIAGSEAVVIGRSDIVGSPVA 177
G +N G L + PCTP G + L++++ ++G E VV+GRS IVGSP A
Sbjct: 125 GLTRINAGRLARGELQRTIFPCTPFGCLYLVQQATGDANFVSGKEVVVLGRSKIVGSPAA 184
Query: 178 TLLTSLDATVTILHSKSKDLREFISRADIVVVAIGQPEFIRGEWFANNKRGAVVIDVGTN 237
L TVTI HSK+ +L+E RADI++VAIG+ F++ +W K GA VID G N
Sbjct: 185 ALFLWHHGTVTICHSKTPNLKEKCLRADILIVAIGRKHFVKADWI---KPGAFVIDCGIN 241
Query: 238 FVKDETRKSGYRNVGDVEFAAAEPYVKLITPVPGGVGPMTVAMLMQNTFESAKR 291
D + Y GDV+ AA+ +TPVPGGVGPMTVAML++NTFE AKR
Sbjct: 242 VGDDPNSRKIY---GDVDTEAAKEVAGFLTPVPGGVGPMTVAMLIRNTFEQAKR 292
>7299133 [H] KOG0089 Methylenetetrahydrofolate
dehydrogenase/methylenetetrahydrofolate cyclohydrolase
Length = 303
Score = 204 bits (518), Expect = 7e-52
Identities = 133/309 (43%), Positives = 174/309 (56%), Gaps = 21/309 (6%)
Query: 3 IIDGKAIANGIRDEIKDEISSLKEKYAVFRPTLTIFQVGNRPDSNTYVKMKLKAAKDAGI 62
IIDGKAIA +R ++ E+ + E +P LT VG P S YV K+ A ++ GI
Sbjct: 4 IIDGKAIAQEVRTQLAHELKGM-EAAGYPKPHLTAVIVGEDPASEKYVANKMVACREVGI 62
Query: 63 DVKFIHLDETVTEVELLGQIDDENSDDSVHGILVQLPLPAHLNEDKITSRVSACKDVDGF 122
+ L + T+ ELL I D N D V GILVQLP+P H+NE I + V KDVDGF
Sbjct: 63 SSETKRLPASTTQEELLQLIADLNKDPQVTGILVQLPVPEHINERTICNAVDVDKDVDGF 122
Query: 123 GPVNIGE--LNKRNGTPYFQPCTPRGIIELLKRSGVTIAGSEAVVIGRSDIVGSPVATLL 180
VNIG L+ P TP G+ LL+ + G AVV+GRS V P+A LL
Sbjct: 123 NEVNIGRTALDMEANI----PATPLGVKRLLEHMKIETLGRNAVVVGRSKNVSLPMAILL 178
Query: 181 --------TSLDATVTILH--SKSKDLREFISRADIVVVAIGQPEFIRGEWFANNKRGAV 230
++DATVTI H + K+L +ADI+VVA+G+P I + K GA
Sbjct: 179 HADGKYATKAMDATVTICHRYTPPKELARHCRQADIIVVAVGKPGLITKDMV---KPGAC 235
Query: 231 VIDVGTNFVKDETRKSGYRNVGDVEFAAAEPYVKLITPVPGGVGPMTVAMLMQNTFESAK 290
VIDVG N +KDE+ ++ VGDV+F ITPVPGGVGPMTVAMLM NT ++A+
Sbjct: 236 VIDVGINRIKDES-TGQFKLVGDVDFEEVRQVAGHITPVPGGVGPMTVAMLMHNTLKAAR 294
Query: 291 RYLDKQLNS 299
+ D + +S
Sbjct: 295 KQFDDRKSS 303
>Hs5729935 [H] KOG0089 Methylenetetrahydrofolate
dehydrogenase/methylenetetrahydrofolate cyclohydrolase
Length = 344
Score = 197 bits (500), Expect = 8e-50
Identities = 125/301 (41%), Positives = 171/301 (56%), Gaps = 17/301 (5%)
Query: 3 IIDGKAIANGIRDEIKDEISSLKEKYAVFRPTLTIFQVGNRPDSNTYVKMKLKAAKDAGI 62
+I G+ +A I+ E++ E+ RP L++ VG P S++YV K +AA GI
Sbjct: 33 VISGRKLAQQIKQEVRQEVEEWVAS-GNKRPHLSVILVGENPASHSYVLNKTRAAAVVGI 91
Query: 63 DVKFIHLDETVTEVELLGQIDDENSDDSVHGILVQLPLPAHLNEDKITSRVSACKDVDGF 122
+ + I +++E ELL I+ N+DD+V G+LVQLPLP H++E +I + VS KDVDGF
Sbjct: 92 NSETIMKPASISEEELLNLINKLNNDDNVDGLLVQLPLPEHIDERRICNAVSPDKDVDGF 151
Query: 123 GPVNIGELNKRNGTPYFQPCTPRGIIELLKRSGVTIAGSEAVVIGRSDIVGSPVATLL-- 180
+N+G + + P TP G+ E++KR+G+ G VV GRS VG P+A LL
Sbjct: 152 HVINVGRMCLDQYS--MLPATPWGVWEIIKRTGIPTLGKNVVVAGRSKNVGMPIAMLLHT 209
Query: 181 ------TSLDATVTILH--SKSKDLREFISRADIVVVAIGQPEFIRGEWFANNKRGAVVI 232
DATVTI H + + L++ ADIV+ A G P I + K GA VI
Sbjct: 210 DGAHERPGGDATVTISHRYTPKEQLKKHTILADIVISAAGIPNLITADMI---KEGAAVI 266
Query: 233 DVGTNFVKDETRKSGYRNVGDVEFAAAEPYVKLITPVPGGVGPMTVAMLMQNTFESAKRY 292
DVG N V D + VGDV+F ITPVPGGVGPMTVAMLM+NT +AK+
Sbjct: 267 DVGINRVHDPVTAKP-KLVGDVDFEGVRQKAGYITPVPGGVGPMTVAMLMKNTIIAAKKV 325
Query: 293 L 293
L
Sbjct: 326 L 326
>Hs13376513_2 [H] KOG0089 Methylenetetrahydrofolate
dehydrogenase/methylenetetrahydrofolate cyclohydrolase
Length = 196
Score = 130 bits (326), Expect = 1e-29
Identities = 86/202 (42%), Positives = 117/202 (57%), Gaps = 18/202 (8%)
Query: 103 HLNEDKITSRVSACKDVDGFGPVNIGELNKRNGTPYFQPCTPRGIIELLKRSGVTIAGSE 162
H++E I + ++ KDVDGF +NIG L + P T + E++KR+G+ G
Sbjct: 1 HVDERTICNGIAPEKDVDGFHIINIGRLCLDQHS--LIPATASAVWEIIKRTGIQTFGKN 58
Query: 163 AVVIGRSDIVGSPVATLLTSL--------DATVTILH--SKSKDLREFISRADIVVVAIG 212
VV GRS VG P+A LL + DATVTI H + + L+ ADI++VA G
Sbjct: 59 VVVAGRSKNVGMPIAMLLHTDGEHERPGGDATVTIAHRYTPKEQLKIHTQLADIIIVAAG 118
Query: 213 QPEFIRGEWFANNKRGAVVIDVGTNFVKDE-TRKSGYRNVGDVEFAAAEPYVKLITPVPG 271
P+ I + K GA VIDVG N+V D T K+ + VGDV+F A + ITPVPG
Sbjct: 119 IPKLITSDMV---KEGAAVIDVGINYVHDPVTGKT--KLVGDVDFEAVKKKAGFITPVPG 173
Query: 272 GVGPMTVAMLMQNTFESAKRYL 293
GVGPMTVAML++NT +AK+ +
Sbjct: 174 GVGPMTVAMLLKNTLLAAKKII 195
>Hs22040978 [H] KOG0089 Methylenetetrahydrofolate
dehydrogenase/methylenetetrahydrofolate cyclohydrolase
Length = 402
Score = 102 bits (254), Expect = 3e-21
Identities = 87/280 (31%), Positives = 124/280 (44%), Gaps = 51/280 (18%)
Query: 3 IIDGKAIANGIRDEIKDEISSLKEKYAVFRPTLTIFQVGNRPDSNTYVKMKLKAAKDAGI 62
+I G+ +A I+ E++ E+ RP L++ VG S++YV K +AA D GI
Sbjct: 154 VISGRKLAQQIKQEVRQEVEEWVAS-GDKRPHLSMILVGESSASHSYVLNKTRAAADVGI 212
Query: 63 DVKFIHLDETVTEVELLGQIDDENSDDSVHGILVQLPLPAHLNEDKITSRVSACKDVDGF 122
N+ S H++E +I + VS KD DGF
Sbjct: 213 -----------------------NTASS-----------RHIDERRICNAVSPDKDADGF 238
Query: 123 GPVNIGELNKRNGTPYFQPCTPRGIIELLKRSGVTIAGSEAVVIGRSDIVGSPVATLL-- 180
+N G + + P TP G+ E++KR+ + G VV GRS V P+A LL
Sbjct: 239 HVINAGRMCLDQYS--MLPATPCGVWEIIKRTDIPTLGKNVVVAGRSKSVEMPIAMLLHT 296
Query: 181 ------TSLDATVTI--LHSKSKDLREFISRADIVVVAIGQPEFIRGEWFANNKRGAVVI 232
DATVTI ++ + L++ ADIV+ A G P I + K A V
Sbjct: 297 DGVHERPGGDATVTISYRYTSKEQLKKHTILADIVISAAGIPNLITADMI---KEEAAVT 353
Query: 233 DVGTNFVKDETRKSGYRNVGDVEFAAAEPYVKLITPVPGG 272
D+G N V D + + VGDV+F ITPVPGG
Sbjct: 354 DMGINRVHDPV-TAKLKLVGDVDFEGVRQKAGYITPVPGG 392
>Hs20541728 [H] KOG0089 Methylenetetrahydrofolate
dehydrogenase/methylenetetrahydrofolate cyclohydrolase
Length = 277
Score = 78.2 bits (191), Expect = 6e-14
Identities = 43/120 (35%), Positives = 71/120 (58%), Gaps = 5/120 (4%)
Query: 9 IANGIRDEIKDEISSLKEKYAVFRPTLTIFQVGNRPDSNTYVKMKLKAAKDAGIDVKFIH 68
I +R E++++++S + P L++ V P S ++ K +AA D GI+ + I
Sbjct: 88 IKQEVRQEVEEQVASGNKG-----PCLSVALVSKTPASLCHILNKTRAAADVGINSEIIV 142
Query: 69 LDETVTEVELLGQIDDENSDDSVHGILVQLPLPAHLNEDKITSRVSACKDVDGFGPVNIG 128
+++E ELL I+ N+D +V G+L+ LPLP H++E KI + V KDVDGF +N+G
Sbjct: 143 KPASISEEELLNLINKLNNDGNVDGLLLHLPLPEHIDERKICNSVFPDKDVDGFHVINVG 202
>YKR080w [H] KOG0089 Methylenetetrahydrofolate
dehydrogenase/methylenetetrahydrofolate cyclohydrolase
Length = 320
Score = 73.9 bits (180), Expect = 1e-12
Identities = 57/197 (28%), Positives = 88/197 (43%), Gaps = 23/197 (11%)
Query: 9 IANGIRDEIKDEISSLKEKYAVFRPTLTIFQVGNRPDSNTYVKMKLKAAKDAGI--DVKF 66
+A EI + + K+ + P L F N P + Y K ++ G D++
Sbjct: 13 VAETFNTEIINNVEEYKKTHNGQGPLLVGFLANNDPAAKMYATWTQKTSESMGFRYDLRV 72
Query: 67 IHLDETVTEVELLGQIDDENSDDSVHGILVQLPLPAHLNEDKITSRVSACKDVDGFGPV- 125
I + + E I N DDSV+GI+V P+ + + + V KDV+G V
Sbjct: 73 IEDKDFLEEA-----IIQANGDDSVNGIMVYFPVFGNAQDQYLQQVVCKEKDVEGLNHVY 127
Query: 126 ------NIGELNKRNGTPYFQPCTPRGIIELLK---------RSGVTIAGSEAVVIGRSD 170
N+ L+K N PCTP I+++L+ G + G + +VI RS+
Sbjct: 128 YQNLYHNVRYLDKENRLKSILPCTPLAIVKILEFLKIYNNLLPEGNRLYGKKCIVINRSE 187
Query: 171 IVGSPVATLLTSLDATV 187
IVG P+A LL + ATV
Sbjct: 188 IVGRPLAALLANDGATV 204
>Hs20542451 [H] KOG0089 Methylenetetrahydrofolate
dehydrogenase/methylenetetrahydrofolate cyclohydrolase
Length = 306
Score = 68.6 bits (166), Expect = 4e-11
Identities = 50/139 (35%), Positives = 72/139 (50%), Gaps = 15/139 (10%)
Query: 113 VSACKDVDGFGPVNIGELNKRNGTPYFQPCTPRGIIELLKRSGVTIAGSEAVVIGRSDIV 172
V++ KDVD F +N+G++ + P P G+ E++K++G+ G VV GRS V
Sbjct: 166 VASDKDVDDFYVINVGQMCLDQYSTL--PAAPWGVWEIIKQTGIPTLGKSVVVAGRSKNV 223
Query: 173 GSPVATLLTS--------LDATVTILH--SKSKDLREFISRADIVVVAIGQPEFIRGEWF 222
PVA LL + DATVTI H + + L++ ADIV+ A P I
Sbjct: 224 EMPVAMLLHTDGAHEGPRGDATVTISHGYTPKEQLKKHTILADIVISAADIPNLITAHMI 283
Query: 223 ANNKRGAVVIDVGTNFVKD 241
K GA +I+VG N V+D
Sbjct: 284 ---KEGAAIINVGINRVQD 299
>Hs17437830 [H] KOG0089 Methylenetetrahydrofolate
dehydrogenase/methylenetetrahydrofolate cyclohydrolase
Length = 106
Score = 61.2 bits (147), Expect = 7e-09
Identities = 34/70 (48%), Positives = 44/70 (62%)
Query: 32 RPTLTIFQVGNRPDSNTYVKMKLKAAKDAGIDVKFIHLDETVTEVELLGQIDDENSDDSV 91
RP L+I VG+ P S+TYV+ K++AA GI + I + V++ ELL D N D V
Sbjct: 23 RPHLSIILVGDNPASHTYVRNKIRAASAVGICSELILKPKDVSQEELLDVTDQLNMDPRV 82
Query: 92 HGILVQLPLP 101
GILVQLPLP
Sbjct: 83 SGILVQLPLP 92
>SPBC1711.04 [H] KOG0089 Methylenetetrahydrofolate
dehydrogenase/methylenetetrahydrofolate cyclohydrolase
Length = 320
Score = 60.5 bits (145), Expect = 1e-08
Identities = 72/326 (22%), Positives = 130/326 (39%), Gaps = 51/326 (15%)
Query: 3 IIDGKAIANGIRDEIKDEISSLKEKYAVFRPTLTIFQVGNRPDSNTYVKMKLKAAKDAGI 62
++ +A +++K ++ + F P L F + P + Y K + G
Sbjct: 15 VVYASRVAETFVEQLKQHVNLFE-----FAPKLVGFLSNSDPAARMYADWTNKTCTEIGF 69
Query: 63 DVKFIHLDETVTEVELLGQIDDENSDDSVHGILVQLPLPAHLNEDKITSRVSACKDVDGF 122
+ V + +L I + N+D SV+GI++ P+ + + VS KDV+G
Sbjct: 70 QYEL----REVPKDDLEDAIVEANNDPSVNGIMIYFPVFNDGQDQYLQQVVSPDKDVEGL 125
Query: 123 GPV-------NIGELNKRNGTPYFQPCTPRGIIELLKRSGV---------TIAGSEAVVI 166
NI L+ PCTP I+++L+ GV + G ++
Sbjct: 126 CHKYVMNMYHNIRHLDPEKTKKSILPCTPLAIVKILEYLGVYNKIINYGNRLYGKTITIV 185
Query: 167 GRSDIVGSPVATLLTSLDATV--------------TILHSKSKDLREFISRADIVVVAIG 212
RS+IVG P+A LL + A V + SK D+ + + + VA
Sbjct: 186 NRSEIVGRPLAALLANDGAKVYSVDIHNVQCFTRGAGIRSKKHDVAD--TNFKLEDVAPI 243
Query: 213 QPEFIRGEWFANNKRGAVVIDVGTNFVKDETRKSGYRNVGDVEFAAAEPYVKLITPVPGG 272
I G AN K +N V+D + + + + A+ + + + P
Sbjct: 244 SDVIICGVPSANYK-------FPSNLVRDGAVCICFSSEKNFDAASLKEHAGIYVP---S 293
Query: 273 VGPMTVAMLMQNTFESAKRYLDKQLN 298
+G +T+AML++N L+K ++
Sbjct: 294 IGKVTIAMLLRNLIRLTSYQLNKPVD 319
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.316 0.136 0.385
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 55,698,156
Number of Sequences: 60738
Number of extensions: 2521479
Number of successful extensions: 6082
Number of sequences better than 1.0e-05: 21
Number of HSP's better than 0.0 without gapping: 17
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 5994
Number of HSP's gapped (non-prelim): 30
length of query: 947
length of database: 30,389,216
effective HSP length: 116
effective length of query: 831
effective length of database: 23,343,608
effective search space: 19398538248
effective search space used: 19398538248
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)