ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactV4304    good          R     KOG2961  General function prediction only                               Predicted hydrolase (HAD superfamily)

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactV4304 1479083 1478517 -189 
         (189 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YHR100c [R] KOG2961 Predicted hydrolase (HAD superfamily)             221   4e-58
SPCC645.02 [R] KOG2961 Predicted hydrolase (HAD superfamily)           98   8e-21
At3g58830 [R] KOG2961 Predicted hydrolase (HAD superfamily)            79   3e-15

>YHR100c [R] KOG2961 Predicted hydrolase (HAD superfamily)
          Length = 185

 Score =  221 bits (564), Expect = 4e-58
 Identities = 101/182 (55%), Positives = 132/182 (72%)

Query: 6   NLSATLNVARLFKDPKLCIPSIKYANFNEIQLPLPSHIKAIVLDKDNCFAKPHDDKVWPE 65
           N+S TLN  RL  +P LC PS+    FN++ +P+   IKA+VLDKDNC A PHDDK+WP+
Sbjct: 2   NISGTLNTLRLLYNPSLCKPSLVVPTFNDLPIPIHDSIKAVVLDKDNCIAFPHDDKIWPD 61

Query: 66  YQEQWDKLKQLYPGKKLLIVSNSAGTNDDIGHIQAKTLEKSTGVPVLLHSIKKPGCHVEI 125
           Y + W+ L+  Y  K LLIVSN+AG+N D  + QAK LE  TG+PVL HS KKPGCH EI
Sbjct: 62  YLQHWETLRSKYSNKALLIVSNTAGSNSDKDYSQAKLLEDKTGIPVLRHSTKKPGCHNEI 121

Query: 126 MAYFKKSKVCEDPSEVAVVGDRLFTDVVMANTMGAYSVWLHEGVIKSSNPFVLLEQQVYN 185
           + YF ++K   +P EVAVVGDRLFTD++MAN MG+Y VW+ +GV  S+NP    E+++YN
Sbjct: 122 LDYFYRNKTITNPKEVAVVGDRLFTDILMANLMGSYGVWIRDGVKVSANPLSKFEKKLYN 181

Query: 186 WL 187
           +L
Sbjct: 182 FL 183


>SPCC645.02 [R] KOG2961 Predicted hydrolase (HAD superfamily)
          Length = 188

 Score = 97.8 bits (242), Expect = 8e-21
 Identities = 54/181 (29%), Positives = 93/181 (50%), Gaps = 13/181 (7%)

Query: 6   NLSATLNVARLFKDPKLCIPSIKYANFNEI----------QLPLPSHIKAIVLDKDNCFA 55
           N+       +  ++P+  IP   +  F++I          +  +P  I+A+VLDKDNC  
Sbjct: 4   NIEGIQAFCQTIRNPRRIIPHATFPTFSQIPCNINYFLEQKFQVPVDIRALVLDKDNCIT 63

Query: 56  KPHDDKVWPEYQEQWDKLKQLYPGKKLLIVSNSAGTN--DDIGHIQAKTLEKSTGVPVLL 113
            P++  +     ++  + + +Y  K ++++SNS GT   D  G + A   +K   +PV+ 
Sbjct: 64  LPNETTIAEAELKKIREFQNIYGEKNVILLSNSIGTRKLDPTGELAAHFQQK-WNIPVVR 122

Query: 114 HSIKKPGCHVEIMAYFKKSKVCEDPSEVAVVGDRLFTDVVMANTMGAYSVWLHEGVIKSS 173
           HS  KP C  E+  Y   +      S++  +GDRL TD+ +AN MG++ VWL  GV  ++
Sbjct: 123 HSKLKPLCTEELYTYLSNNSHVSSASQILFIGDRLLTDITLANIMGSWGVWLTRGVGNTT 182

Query: 174 N 174
           N
Sbjct: 183 N 183


>At3g58830 [R] KOG2961 Predicted hydrolase (HAD superfamily)
          Length = 348

 Score = 79.3 bits (194), Expect = 3e-15
 Identities = 55/171 (32%), Positives = 90/171 (52%), Gaps = 11/171 (6%)

Query: 12  NVARLFKDPKLCIP--SIKYANFNEIQLPLPSHIKAIVLDKDNCFAKPHDDKVWPEYQEQ 69
           +V+ + KD +  +P  S+K   + + ++      K +V DKDN    P+   +WP  +  
Sbjct: 145 SVSVVVKDRQFVLPHVSVKDLRYIDWEVLKRKGFKGVVFDKDNTLTAPYSLAIWPPLRPS 204

Query: 70  WDKLKQLYPGKKLLIVSNSAG-TNDDIGHIQAKTLEKSTGVPVLLHSIKKP-GCHVEIMA 127
            ++ K ++ G  + + SNSAG T  D    +AK LE   G+ VL H +KKP G   E+  
Sbjct: 205 IERCKAVF-GHDIAVFSNSAGLTEYDHDDSKAKALEAEIGIRVLRHRVKKPAGTAEEVEK 263

Query: 128 YFKKSKVCEDPSEVAVVGDRLFTDVVMANTMGAYSVWLHEGVIKSSNPFVL 178
           +F  +      SE+ +VGDR FTD+V  N  G  +V L E + ++  PF++
Sbjct: 264 HFGCTS-----SELIMVGDRPFTDIVYGNRNGFLTV-LTEPLSRAEEPFIV 308


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.319    0.136    0.417 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,214,268
Number of Sequences: 60738
Number of extensions: 527210
Number of successful extensions: 1206
Number of sequences better than 1.0e-05: 3
Number of HSP's better than  0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 1201
Number of HSP's gapped (non-prelim): 3
length of query: 189
length of database: 30,389,216
effective HSP length: 100
effective length of query: 89
effective length of database: 24,315,416
effective search space: 2164072024
effective search space used: 2164072024
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)