ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV4309 good O KOG2231 Posttranslational modification, protein turnover, chaperones Predicted E3 ubiquitin ligase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV4309 1479322 1481289 656
(656 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDR266c [O] KOG2231 Predicted E3 ubiquitin ligase 390 e-108
SPCC1223.01 [O] KOG2231 Predicted E3 ubiquitin ligase 162 2e-39
7291808 [O] KOG2231 Predicted E3 ubiquitin ligase 154 3e-37
At3g62240 [O] KOG2231 Predicted E3 ubiquitin ligase 136 1e-31
CE04262_2 [O] KOG2231 Predicted E3 ubiquitin ligase 126 1e-28
ECU01g0780 [O] KOG2231 Predicted E3 ubiquitin ligase 120 7e-27
Hs22068519 [O] KOG2231 Predicted E3 ubiquitin ligase 117 6e-26
At2g47090 [O] KOG2231 Predicted E3 ubiquitin ligase 100 1e-20
>YDR266c [O] KOG2231 Predicted E3 ubiquitin ligase
Length = 639
Score = 390 bits (1001), Expect = e-108
Identities = 220/559 (39%), Positives = 316/559 (56%), Gaps = 47/559 (8%)
Query: 88 VCLICAEKLHIIALSPCNHPTCHKCCLRQRALYEKKQCLFCRSEVEDIIFTDKIKETYEH 147
+C+ICA KL ++L+PC+H TCH C RQRALY KK CL CR+E E+++FTD+I
Sbjct: 63 LCVICARKLTYVSLTPCHHKTCHICGFRQRALYNKKSCLICRTENEEVMFTDRIDGD--- 119
Query: 148 LDKKAKLEFKNEKLGIRFTSKNVMNDAMNLLKYVSPFGDANK--DFGSFKKLNEFLRQEH 205
+ K KNEK GI FTS+ V + +NLLK+ P + DFGSFKK NE L+ EH
Sbjct: 120 ISDKYNFCEKNEKYGINFTSEEVATETLNLLKFFCPLSKDEQVCDFGSFKKYNEHLKSEH 179
Query: 206 NKTICMICAGHKKAFPGELKVMTVKQLKIHESRGDPKDGFKGHPMCGFCSGKRFYSDDEL 265
N+ IC+ICA HK AFP EL++ T QL+ H+++G+ +GFKGHPMC FCSGKRFYSDDEL
Sbjct: 180 NRMICLICATHKHAFPCELEIFTQNQLRNHQTKGN-SEGFKGHPMCAFCSGKRFYSDDEL 238
Query: 266 YKHMKLSHERCHICDQLDPSQPQYFKNYEDLFEHFKSAHYVCTVPSCLDAKFVVFSDELD 325
Y HM+ HE+CHICD+++P+ PQYFK+Y LF+HFK +HYVCTV +CLD KFVVF DEL+
Sbjct: 239 YIHMRNQHEKCHICDKMNPASPQYFKDYNQLFDHFKHSHYVCTVQTCLDNKFVVFKDELE 298
Query: 326 LRAHIIKEHPHLAGDSRRAMTLIDNTSKKFRSQITTF-SNPTRIIDAQNE---------- 374
L+AHI++EH + ++ K F+S+++TF S P+R+I +++
Sbjct: 299 LQAHILQEHGN----------ILKGKPKFFQSELSTFISAPSRVIRERDDYDLPSISSLP 348
Query: 375 --------ESNSANSRDVKRRRMEQRARHYLGNSEPDFQSFLIINDLFNKNEISAQELQS 426
+ SA+S + R R+ +RA++YL NS+ DF F N+ ++K +SA++L
Sbjct: 349 GSSSGSRTDVRSASSPEESRLRLAERAKYYLENSKEDFNKFSSYNEDYSKGRLSAEKLLE 408
Query: 427 KYEDLFKQHADDIQLLIYDLSELYHSTSDKHKRLRAIYEGIMKKKENAGKFKPLVADSSS 486
Y+ LF + D+ LLI++L+E + S K+ L AIYE + L +DSS
Sbjct: 409 SYKLLFTKPNADVYLLIHNLAETFPKNSSKYNNLNAIYEQREQTLARQTSLPSLSSDSSL 468
Query: 487 SVNXXX---XXXXXXXXXXXXXXXXXFTSLPAVSEPIFSSKASYTNLRKNGXXXXXXXVI 543
S++ +LP + P S T ++KN +
Sbjct: 469 SMSIGRGHWGGTNDGGSAGAALGVRNIKNLPTLKSPSASYDPFATTVKKN-TLRPVQNIK 527
Query: 544 RSGXXXXXXXXXXXXYAKKN---QSPPGSSTVVVNSQPNVTAIKSPNPTSFPKLQATKKK 600
R+ A +S GSS+ +N+ + +KS N P+L K K
Sbjct: 528 RTTPQSVSYRTSTNTVAFSPTYLESKKGSSSTSLNNSKD--KLKSLN---LPQLPPPKPK 582
Query: 601 FVAPPVKQTTIPNPSTWGK 619
P + + I +P WGK
Sbjct: 583 VQIPGLNRPQIADPKQWGK 601
>SPCC1223.01 [O] KOG2231 Predicted E3 ubiquitin ligase
Length = 299
Score = 162 bits (409), Expect = 2e-39
Identities = 88/221 (39%), Positives = 125/221 (55%), Gaps = 10/221 (4%)
Query: 88 VCLICAEKLHIIALSPCNHPTCHKCCLRQRALYEKKQCLFCRSEVEDIIFTDKIKETYEH 147
+C ICAE + + PCNH CH C LR RALY+ K+C FC++E + ++ T K E H
Sbjct: 80 ICFICAEGITYSCVLPCNHRMCHVCALRLRALYKTKECTFCKTEWDTVLIT-KDHEIDIH 138
Query: 148 LDKKAKLEFKNEKLGIRFTSKNVMNDAMNLLKYVSPFGDANKDFGSFKKLNEFLRQEHNK 207
AKL F++EKLGI ++ ++ ++ LL++ P + + L + +H+K
Sbjct: 139 DVDLAKLPFQDEKLGIVYSDEHAQEESNLLLQFNCPEDACDITCKGWFDLKLHAKVKHHK 198
Query: 208 TICMICAGHKKAFPGELKVMTVKQLKIHESRGDPKD-----GFKGHPMCGFCSGKRFYSD 262
C +C +KK F E + + K L H GD GFKGHP C FC+ FY D
Sbjct: 199 FFCDLCVKNKKVFTHEHTLFSKKGLTKHNEVGDQGSDLEITGFKGHPKCEFCN-THFYDD 257
Query: 263 DELYKHMKLSHERCHICDQLDPSQP--QYFKNYEDLFEHFK 301
DEL+KH + HERC+ICDQ+ +P QYFKNY+ L HF+
Sbjct: 258 DELFKHCREKHERCYICDQV-AGRPTHQYFKNYDSLERHFE 297
>7291808 [O] KOG2231 Predicted E3 ubiquitin ligase
Length = 867
Score = 154 bits (390), Expect = 3e-37
Identities = 75/249 (30%), Positives = 134/249 (53%), Gaps = 9/249 (3%)
Query: 89 CLICAEKLHIIALSPCNHPTCHKCCLRQRALYEKKQCLFCRSEVEDIIFT-DKIKETYEH 147
C++C + + I ++ C+HP C++C R R L ++ +C CR + ++FT DK+ Y
Sbjct: 48 CVVCFKNVEIFSIGDCDHPVCYECSTRMRVLCQQNECPICRHVLSKVLFTLDKL--PYRE 105
Query: 148 LDKKAKLEFKNEKLGIRFTSKNVMNDAMNLLKYVSPFGDANKDFGSFKKLNEFLRQEHNK 207
L+ + + ++K I F + + LL + P DA + +F+ L +R EH
Sbjct: 106 LEANNRSDLYSKKYRIGFCTAEIRQQFFKLLDHPCPKCDA-PPYRTFEGLRNHVRSEHKL 164
Query: 208 TICMICAGHKKAFPGELKVMTVKQLKIHESRGDPKD-GFKGHPMCGFCSGKRFYSDDELY 266
C +C F E + T +L++H ++GDP + +GHP+C +C+ KR+ DEL+
Sbjct: 165 LYCDLCVETLNIFTFERRCYTQAELQLHNTKGDPDNRSHRGHPLCEYCN-KRYVDRDELF 223
Query: 267 KHMKLSHERCHICDQLDPSQPQYFKNYEDLFEHFKSAHYVCTVPSCLDAKFV-VFSDELD 325
+H++ H CH CD +++ +Y DL EHF++ H++C C +FV F +E++
Sbjct: 224 RHLRREHYFCHFCDA--DGCNEFYNDYADLAEHFRAEHFLCEEGKCATEQFVGAFRNEIE 281
Query: 326 LRAHIIKEH 334
+AH+ H
Sbjct: 282 YKAHVANVH 290
>At3g62240 [O] KOG2231 Predicted E3 ubiquitin ligase
Length = 812
Score = 136 bits (342), Expect = 1e-31
Identities = 141/584 (24%), Positives = 216/584 (36%), Gaps = 88/584 (15%)
Query: 89 CLICAEKLHIIALSPCNH-PTCHKCCLRQRALYEKKQCLFCRSEVEDIIFTDKIKETYE- 146
C +CA+ L +A C H C C +R R + + +C C++E I+F K Y
Sbjct: 5 CAVCADNLEWVAYGSCGHREVCSTCVVRLRFVLDDPRCCICKTE-SPIVFVTKALGDYTR 63
Query: 147 ----------------------HLDKKAKLEFKNEKLGIRFTSK---NVMNDAMNLLKYV 181
H D +A + ++ I+ + V + + +
Sbjct: 64 TINDFSTFPSAPREGRVGAFWYHEDTQAFFDDLDQYRMIKAMCRLSCGVCDKTEDKPREG 123
Query: 182 SPFGDANKDFGSFKKLNEFLRQEHNKTICMICAGHKKAFPGELKVMTVKQLKIHESRGDP 241
P + S ++L L +H +C +C +K F E K+ T QL H GD
Sbjct: 124 GPRHHRQR-IKSVEQLKGHLYHKHKLHMCGLCLEGRKIFICEQKLYTRAQLNQHIQTGDS 182
Query: 242 --------KDGFKGHPMCGFCSGKRFYSDDELYKHMKLSHERCHICDQLDPSQPQYFKNY 293
+ GF GHPMC FC FY D+ELY HM H CH+C + P Q +Y+KNY
Sbjct: 183 EVDGSESERGGFAGHPMCEFCRNP-FYGDNELYTHMSTEHYTCHLCQRSQPGQYEYYKNY 241
Query: 294 EDLFEHFKSAHYVCTVPSCLDAKFVVFSDELDLRAHIIKEHPHLAGDSRRAMTLIDNTSK 353
+DL HF+ H++C SCL KF VF +E +L+ H EH S+R+ L
Sbjct: 242 DDLEIHFRRDHFLCEDDSCLAKKFTVFQNESELKRHNAIEHGGKMSRSQRSAAL------ 295
Query: 354 KFRSQITTFSNPTRIIDAQNEESNSANSRDVKRRRMEQRARHYLGNSEPDFQSFLIINDL 413
QI T +R D +N RR + R G+ E + +
Sbjct: 296 ----QIPTSFRYSRGNDQEN------------RRGRPRSFRREPGDEEYNLAVHAALR-- 337
Query: 414 FNKNEISAQE-------LQSKYEDLFKQHADDIQLLIYDLSELYHSTSDKHKRLRAIYE- 465
+++E S QE + + H DD LI + L ST+D R +
Sbjct: 338 LSESEYSRQEPAPPPSSAPPGFSENNNIHVDDTDPLIQPMESL--STTDMEPSSRYLQAV 395
Query: 466 ---GIMKKKENAGKFKPLVADSSSSVNXXXXXXXXXXXXXXXXXXXXFT----SLPAVSE 518
G + F PL SS N T + P+
Sbjct: 396 GSFGGGGSRLGESAFPPLSGQQSSGQNVESLPTNTMAARLRRQTNRTSTASAIASPSQGW 455
Query: 519 PIFSSKASYTNLRKNGXXXXXX--XVIRSGXXXXXXXXXXXXYAKKNQSPPGSSTVVVNS 576
P+ + ++ G + R+ + + +Q P +S V
Sbjct: 456 PVINRGPGQASITSGGNHSSSGWPAIGRTPVQASSSSVQSRSHNRVSQPRPLASAV---P 512
Query: 577 QPNVTAIKSPNPTSFPKLQATKKKFVA----PPVKQTTIPNPST 616
Q A + P+ +S P L T+ + PPV + N T
Sbjct: 513 QAARNANRIPHSSSAPNLSDTRSLQPSHSDFPPVSSAVVQNRKT 556
>CE04262_2 [O] KOG2231 Predicted E3 ubiquitin ligase
Length = 837
Score = 126 bits (316), Expect = 1e-28
Identities = 71/257 (27%), Positives = 116/257 (44%), Gaps = 18/257 (7%)
Query: 89 CLICAEKLHIIALSPCNHPTCHKCCLRQRALYEKKQCLFCRSEVEDIIF--TD------- 139
C IC +K + + C HP C +C +R R L K C CRS+++ + F TD
Sbjct: 1 CDICCKKNDVFGIGSCRHPVCAECVIRMRILGNSKTCPVCRSDIDILSFCTTDDDLANVP 60
Query: 140 -KIKETYEHLDKKAKLEFKNEKLGIRFTSKNVMNDAMNLLKYVSPFGDANKDFGSFKKLN 198
K+T + + + F N+ G ++ ++ K +F SF L
Sbjct: 61 LSFKKTAHPDEDRYDIRFSNKIAGTKYEKY-----LAHVCKICKTDDGERLEFPSFMSLR 115
Query: 199 EFLRQEHNKTICMICAGHKKAFPGELKVMTVKQLKIHESRGDPKD-GFKGHPMCGFCSGK 257
+ + H ++ C IC + F E K T QL+ H GD D FKGHP C FC +
Sbjct: 116 QHMASRHEQSYCHICTDNLNLFSRERKTYTRDQLQRHMRSGDFDDKSFKGHPQCLFCE-Q 174
Query: 258 RFYSDDELYKHMKLSHERCHICDQLDPSQPQYFKNYEDLFEHFKSAHYVCTVPSCLDAKF 317
+F ++ Y+H++ H C C+ +F ++ L +H+K HY+C C +
Sbjct: 175 KFLDEENRYRHLRKDHFFCQFCESDGTMTNVFFGKHDGLKKHYKEHHYICETEECREMG- 233
Query: 318 VVFSDELDLRAHIIKEH 334
+ F+++ +L H EH
Sbjct: 234 IAFANKFELDLHRANEH 250
>ECU01g0780 [O] KOG2231 Predicted E3 ubiquitin ligase
Length = 428
Score = 120 bits (301), Expect = 7e-27
Identities = 69/246 (28%), Positives = 117/246 (47%), Gaps = 8/246 (3%)
Query: 89 CLICAEKLHIIALSPCNHPTCHKCCLRQRALYEKKQCLFCRSEVEDIIFTDKIKETYEHL 148
C IC + A C H C C +R LY + C CR E ++F+
Sbjct: 3 CQICTGSTDVSARYDCGHQICMACAVRLVFLYMQSGCPLCRGTTEAVVFSASSPACGT-- 60
Query: 149 DKKAKLEFKNEKLGIRFTSKNVMNDAMNLLKYVSPFGDANKDFGSFKKLNEFLRQEHNKT 208
+ + + + + + + +V + +LL + ++ FGS K+L + Q H
Sbjct: 61 -SRRQWKGREAPVAVFYEGADVRDRMEDLLS--NKCQKCHRRFGSLKELKKHYAQ-HGFV 116
Query: 209 ICMICAGHKKAFPGELKVMTVKQLKIHESRGDPKDGFKGHPMCGFCSGKRFYSDDELYKH 268
+C C G++K F E+++ ++ H++ ++GF GH +C C F SDD +H
Sbjct: 117 LCSECIGNRKDFWNEIRLYRSSTIRDHKNGSLEEEGFSGHVLCIHCKIYLFDSDDAR-RH 175
Query: 269 MKLSHERCHICDQLDPSQPQYFKNYEDLFEHFKSAHYVCTVPSCLDAKFVVFSDELDLRA 328
L HE CH+CD + + QY+ ++EDL H+++AHY CT +C K VF +L
Sbjct: 176 CNLRHEACHVCDMVG-IRYQYYSSFEDLEAHYRNAHYCCTFQTCRVGKCYVFPYHTELLE 234
Query: 329 HIIKEH 334
H+ + H
Sbjct: 235 HLTRFH 240
>Hs22068519 [O] KOG2231 Predicted E3 ubiquitin ligase
Length = 849
Score = 117 bits (293), Expect = 6e-26
Identities = 72/229 (31%), Positives = 110/229 (47%), Gaps = 8/229 (3%)
Query: 117 RALYEKKQCLFCRSEVEDIIFTDKIKETYEHLDKKAKLEFKNEKLGIRFTSKNVMNDAMN 176
R L E++ C CR E+ ++F K+ + + E +K I F V
Sbjct: 2 RVLCEQRYCAVCREELRQVVFGKKLPAFATIPIHQLQHE---KKYDIYFADGKVYALYRQ 58
Query: 177 LLKYVSPFGDANKDFGSFKKLNEFLRQEHNKTICMICAGHKKAFPGELKVMTVKQLKIHE 236
LL++ P F F L + +R++H C +C H + F E K + K L H
Sbjct: 59 LLQHECPRCPELPPFSLFGDLEQHMRRQHELFCCRLCLQHLQIFTYERKWYSRKDLARHR 118
Query: 237 SRGDPKD-GFKGHPMCGFCSGKRFYSDDELYKHMKLSHERCHICDQLDPSQPQYFKNYED 295
+GDP D +GHP+C FC +R+ +DEL KH++ H CH CD D +Q Y+ +Y
Sbjct: 119 MQGDPDDTSHRGHPLCKFCD-ERYLDNDELLKHLRRDHYFCHFCDS-DGAQ-DYYSDYAY 175
Query: 296 LFEHFKSAHYVCTVPSCLDAKFV-VFSDELDLRAHIIKEHPHLAGDSRR 343
L EHF+ H++C C +F F E+DL+AH H ++R+
Sbjct: 176 LREHFREKHFLCEEGRCSTEQFTHAFRTEIDLKAHRTACHSRSRAEARQ 224
>At2g47090 [O] KOG2231 Predicted E3 ubiquitin ligase
Length = 737
Score = 99.8 bits (247), Expect = 1e-20
Identities = 75/289 (25%), Positives = 121/289 (40%), Gaps = 42/289 (14%)
Query: 89 CLICAEKLHIIALSPCNH-PTCHKCCLRQRALYEKKQCLFCRSEVEDIIFTDKIKE---- 143
C +CAE L + C H C C +R R + ++C C++E + T + +
Sbjct: 5 CAVCAENLEWVGYGSCGHREVCSTCVVRLRFILNDRRCCICKTECPVVFVTKALGDYTKT 64
Query: 144 ------TYEHLDKKAKLE--FKNEKLGIRFTSKNVMNDAMNLLKYVSPF-GDANKD---- 190
T+ + K+ ++ + +E+ + F N + + D NK
Sbjct: 65 ISDFSTTFPSVPKEGRVGSFWYHEETNVYFDDLNHYTRIKAMCRLSCNLCNDTNKTRPKK 124
Query: 191 -------FGSFKKLNEFLRQEHNKTICMICAGHKKAFPGELKVMTVKQLKIHESRGDP-- 241
F S + L + L +H +C +C +K F E K+ T QL H S GD
Sbjct: 125 EPNHCVRFKSVEHLKDHLNHQHKLHMCSLCLVGRKVFICEQKLFTKGQLNQHISSGDSEV 184
Query: 242 ------KDGFKGHPMCGFCSGKRFYSDDELYKHMKLSHERCHICDQLDPSQPQYFKNYED 295
+ GF GHPM F L +S C +L P Q +Y+ NY+D
Sbjct: 185 DGSESERGGFTGHPM--------FVRVMNLIDLEMISFSSIWQC-RLKPGQYEYYGNYDD 235
Query: 296 LFEHFKSAHYVCTVPSCLDAKFVVFSDELDLRAHIIKEHPHLAGDSRRA 344
L HF+S H++C +CL KF+VF E +L+ ++P+ RR+
Sbjct: 236 LEVHFRSDHFLCEDETCLAKKFIVFQIEAELKIQASFQYPNSRRGRRRS 284
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.132 0.395
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 35,956,109
Number of Sequences: 60738
Number of extensions: 1526258
Number of successful extensions: 4935
Number of sequences better than 1.0e-05: 8
Number of HSP's better than 0.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 4896
Number of HSP's gapped (non-prelim): 10
length of query: 656
length of database: 30,389,216
effective HSP length: 113
effective length of query: 543
effective length of database: 23,525,822
effective search space: 12774521346
effective search space used: 12774521346
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)