ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV4386 good S KOG1032 Function unknown Uncharacterized conserved protein, contains GRAM domain
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV4386 1507520 1503513 -1336
(1336 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDR326c [S] KOG1032 Uncharacterized conserved protein contains G... 614 e-175
YHR080c [S] KOG1032 Uncharacterized conserved protein contains G... 364 e-100
SPBC20F10.07 [S] KOG1032 Uncharacterized conserved protein conta... 239 2e-62
YFL042c [S] KOG1032 Uncharacterized conserved protein contains G... 196 2e-49
YLR072w [S] KOG1032 Uncharacterized conserved protein contains G... 158 6e-38
Hs22052111 [S] KOG1032 Uncharacterized conserved protein contain... 111 9e-24
CE15185 [S] KOG1032 Uncharacterized conserved protein contains G... 89 6e-17
Hs12965197 [S] KOG1032 Uncharacterized conserved protein contain... 88 1e-16
Hs20482574 [S] KOG1032 Uncharacterized conserved protein contain... 80 2e-14
At1g02120 [S] KOG1032 Uncharacterized conserved protein contains... 73 3e-12
Hs22042713 [R] KOG4347 GTPase-activating protein VRP 60 2e-08
At3g59660 [S] KOG1032 Uncharacterized conserved protein contains... 55 7e-07
>YDR326c [S] KOG1032 Uncharacterized conserved protein contains GRAM domain
Length = 1438
Score = 614 bits (1584), Expect = e-175
Identities = 351/913 (38%), Positives = 512/913 (55%), Gaps = 71/913 (7%)
Query: 449 LTMRSLSPA-AATKLFTA-PLRNSFSGRNMTRASSISKTSDIVQPPGGPTRLSTSD---I 503
L RS SP+ K+ LRNSF+ +N +S T+ Q P T S + +
Sbjct: 566 LNRRSFSPSNLGNKVIPGINLRNSFN-KNRNSSSDFFSTNQGQQMPRTSTAGSGNIHAIM 624
Query: 504 PIKRKTEEPELQGIEYASAKRNTDFHTIFKDTVVSPIERLIADYSCALSKDILIQGRLYI 563
+ ++ +L+GIEYAS K+N++FHT+FKD ++P E+LI D+SCALS+DIL+QGR+YI
Sbjct: 625 GLDSGNDDFKLEGIEYASEKKNSEFHTLFKDCDINPNEKLIVDHSCALSRDILLQGRMYI 684
Query: 564 SDRHICFYSNILGWMKTVVVPFKEIVQIEQKNTAVLFPNAISIQTLHDKFLFASFISRDS 623
SD HI F+SNILGW+ TV +PFKEIVQIE+K TA +FPN I I TLH K++FASF+SRD+
Sbjct: 685 SDAHIGFFSNILGWVSTVFIPFKEIVQIEKKTTAGIFPNGIVIDTLHTKYIFASFMSRDA 744
Query: 624 TFDQIMDIWNQTIINKNFKPKRGTMGSADLMNDENLTLDSEAS----SMDYDDEDVQTND 679
TFD I D+WNQ I+ K ++ G ND+ DS ++ S D DD+ +D
Sbjct: 745 TFDLITDVWNQIILGKKYRNGFGN-------NDDGTISDSSSAFFDDSDDNDDDGDLDDD 797
Query: 680 RTSTSITDSYGNRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXARXXXXXXXXXXXX 739
+ TD
Sbjct: 798 DPDINSTDM---------------------------TSSDDIDADVFNESNDLGKNQKST 830
Query: 740 XXXXXXXKHEPTTPDYTPKENEREMGETILNAPLGTVINILYGSDVQYFDRILKAQGNYD 799
KH PTT D+ P N+ + E +NAPLG V+N+LYG DV Y++RILKAQ N++
Sbjct: 831 NYLLGPNKHSPTTADFKPSNNDHLVIEANINAPLGKVVNLLYGEDVSYYERILKAQKNFE 890
Query: 800 ISKF-NEIIQSKKREYVYTKPISGSIGPSKTRCEITESIDKLDLKNYVQVTQVSKTPDIP 858
IS N + K R+Y YTKP+SGSIGPSKT+C IT++++ DL++YV+V ++K PD+P
Sbjct: 891 ISPIPNNFLTKKIRDYAYTKPLSGSIGPSKTKCLITDTLEHYDLEDYVKVLSITKNPDVP 950
Query: 859 SGNSFVVKTIFLFCWADDNSTKVKVYTYVDWSAKSWIKAAVEKGTFDGIKSSTKXXXXXX 918
SGN F VKT+FLF W +NSTK+ VY VDW+ KSWIK+ +EKGTFDG+ +TK
Sbjct: 951 SGNIFSVKTVFLFSWDKNNSTKLTVYNSVDWTGKSWIKSMIEKGTFDGVADTTKIMISEI 1010
Query: 919 XXXXXXNKGKSSSPD--NRKDIEEVLSSLPCLGPKEHEPTECTFEKSSGYEIIDDKISFK 976
+S + + EE + +LP +GP H+PTE F+K +ID+KI+
Sbjct: 1011 KKILSDEDSNINSKHQASNNESEEEIINLPTIGPPVHDPTEPDFQKGKDDTVIDEKINIP 1070
Query: 977 SPVGTIFQLICGDDTSYMKRILERQKNIEISDIPPFQNKTRKYTYVKPLPGSIGPKQAKC 1036
P+GT+F L+ GDDTSY+K+I+E Q N + DIP F N R+ TY K L S GPKQ KC
Sbjct: 1071 VPLGTVFSLLYGDDTSYIKKIIENQNNFNVCDIPKFVNNAREITYTKKLNNSFGPKQTKC 1130
Query: 1037 YIEEKIESDDVNTCMVLEQISKTPEIPSGNSFEVHTKFYFSWGEKNTTNVLAATNIEWTG 1096
+ E IE D+N+ +++QI ++P++P G+SF VHT+F++SWG+ NTTN+ TN+ WTG
Sbjct: 1131 IVTETIEHMDLNSFFMVKQIVRSPDVPYGSSFSVHTRFFYSWGDHNTTNMKVVTNVVWTG 1190
Query: 1097 KSWIKSAIQKGSINGQKASVQDMVAEIKDILSSASSTTKRRPTXXXXXXXXXXXXXXXXX 1156
KS +K I+KGSI+GQ++S + +V ++K I+S+ASS TK++
Sbjct: 1191 KSMLKGTIEKGSIDGQRSSTKQLVDDLKKIISNASS-TKKKSRRRGKTVNKRKSSPSTIK 1249
Query: 1157 XXXXQPEVQNGGLYATVQEIYGIVKDXXXXXXXXXXXXXXXXXXXXXXFVPRLCSSLFH- 1215
+ ++ + + +++ F RL LFH
Sbjct: 1250 NEKNEENFEDTSTKNSFFSAFSMLQQVNITSVQGIMTIISFFICLIFFF--RL---LFHS 1304
Query: 1216 -RSPSPHFVSPSTIVMDGEEYYVLPAMETYY----------GRNRRGNKNKPFSKLYSDS 1264
+ + ++P TI+++G EY +P +T Y R + NKN + +D+
Sbjct: 1305 KNTSNIQIITPGTILINGNEYNYVPNFKTLYHVYEDNIIKDARRKDSNKN----NIVTDT 1360
Query: 1265 EYDLWNW-IDSHSGSELNKKSGPNLDVNYNINSTIPLNKAVEHKKQNLKEVIRLTELQLM 1323
E +W+W ID +G+ N ++ + N + +N +HK Q L E I++TELQL
Sbjct: 1361 EGLIWDWLIDRGNGTVQNSVLSNHIKESNNKKVKL-VNGVSDHKIQQLVESIKITELQLQ 1419
Query: 1324 KLKEKANLTDNST 1336
++KE TDN++
Sbjct: 1420 EMKELLAQTDNTS 1432
>YHR080c [S] KOG1032 Uncharacterized conserved protein contains GRAM domain
Length = 1345
Score = 364 bits (935), Expect = e-100
Identities = 215/608 (35%), Positives = 319/608 (52%), Gaps = 51/608 (8%)
Query: 748 HEPTTPDYTPKENEREMGETILNAPLGTVINILYGSDVQYFDRILKAQGNYDISKFNEII 807
H PT Y P NE+ + E+ ++A LG V+NIL+G DV Y ILKAQ N DIS ++
Sbjct: 746 HSPTETVYKPAPNEKLVNESTIHASLGRVVNILFGKDVSYIMAILKAQKNSDISPIPVLV 805
Query: 808 QS------KKREYVYTKPISGSIGPSKTRCEITESIDKLDLKNYVQVTQVSKTPDIPSGN 861
S KKR+Y Y K G+IGP KT+C ITE+I +L+ YVQV Q +KTPD+PSGN
Sbjct: 806 DSPTVSEGKKRDYSYVKTTPGAIGPGKTKCMITETIQHFNLEEYVQVLQTTKTPDVPSGN 865
Query: 862 SFVVKTIFLFCWADDNSTKVKVYTYVDWSAKSWIKAAVEKGTFDGIKSSTK--XXXXXXX 919
SF V+T++L WA++N TK+K+Y V+W+ KS IK+ +EKGTFDG+ +TK
Sbjct: 866 SFYVRTVYLLSWANNNETKLKLYVSVEWTGKSLIKSPIEKGTFDGVTDATKILVEELGNI 925
Query: 920 XXXXXNKGKSSSPDNRKDIEEVLSSLPCLGPKEHEPTECTFEKSSGYEIIDDKISFKSPV 979
K K SS +N + S+LP + P H PTE +K II + + +P+
Sbjct: 926 LTRSATKRKRSSKENTVTV----STLPKMEPSSHAPTEPDIQKDKDDSIIRENENIPAPL 981
Query: 980 GTIFQLICGDDTSYMKRILERQK-NIEISDIPPF-----QNKTRKYTYVKPLPGSIGPKQ 1033
GT+ QL+ G +T YM++++ R K N+ + IP F + +R Y Y K L SIGPKQ
Sbjct: 982 GTVVQLLFGSNTEYMQKVITRDKNNVNVETIPKFTPSLVEGGSRHYEYTKKLNNSIGPKQ 1041
Query: 1034 AKCYIEEKIESDDVNTCMVLEQISKTPEIPSGNSFEVHTKFYFSWGEKNTTNVLAATNIE 1093
KC + E IE D+N +++ Q +KTP++PSG++F V +K + WG+ +TTN+ T I
Sbjct: 1042 TKCLLTESIEHMDINNYVLVTQTTKTPDVPSGSNFAVESKIFLFWGQHDTTNMTVITKIN 1101
Query: 1094 WTGKSWIKSAIQKGSINGQKASVQDMVAEIKDILSSASSTTKRRPTXXXXXXXXXXXXXX 1153
WT KS++K AI+KGS+ GQK SV M++E++DI+S A S ++P
Sbjct: 1102 WTSKSFLKGAIEKGSVEGQKVSVDYMLSELRDIISRAKS---KKPVKKVMKSHDKHRPFH 1158
Query: 1154 XXXXXXXQPEVQNGGLYATVQEIYGIVKDXXXXXXXXXXXXX------XXXXXXXXXFVP 1207
++ + I V++ + P
Sbjct: 1159 SKVEQKSSESRKSDDNKDILTHILDFVQNNFSSEIFMNKLLSPQKLFLILGLTIMLFWSP 1218
Query: 1208 RLCSSLFHRSPSPHFVSPSTIVMDGEEYYVLPAMETYYG--RNRRGNKNKPFSKLYS--- 1262
RL +F + + P +++DG+EY +P+ T Y N +K K + Y+
Sbjct: 1219 RL--HVFQEKNNLQIIKPGRLLIDGQEYNYVPSFGTLYNSYENAISSKKKRENVNYARDK 1276
Query: 1263 ----DSEYDLWNWIDSHSGSELNKKSGPNLDVNYNINSTIPLNKAVEHKKQNLKEVIRLT 1318
E D+W+WI S+ GS ++ + L EHK Q L E I++T
Sbjct: 1277 SPIVGRESDIWDWI-SNRGSAISPR------------GRAMLRNDDEHKLQQLSESIKIT 1323
Query: 1319 ELQLMKLK 1326
E+QL +K
Sbjct: 1324 EMQLNHMK 1331
Score = 193 bits (491), Expect = 1e-48
Identities = 106/242 (43%), Positives = 150/242 (61%), Gaps = 16/242 (6%)
Query: 451 MRSLSPAAATKLFTAPLRNSFSGRNMTRASSISKTS---DIVQPPGGPTRLSTSDIPIKR 507
+++ + A+T + + N + R+SS S +S +I++P R S+ I
Sbjct: 481 VKNSTDIASTIIPRPSMSNGRPSSGLRRSSSKSFSSTPVNIIEPSEENGRQSSIRI---- 536
Query: 508 KTEEPELQGIEYASAKRNTDFHTIFKDTVVSPIERLIADYSCALSKDILIQGRLYISDRH 567
+G+EYAS K++ +FH IFKD+ VSP ERLI D+SCALS+DIL+QGR+YISD+H
Sbjct: 537 -------KGVEYASEKKDAEFHAIFKDSGVSPNERLILDHSCALSRDILLQGRMYISDQH 589
Query: 568 ICFYSNILGWMKTVVVPFKEIVQIEQKNTAVLFPNAISIQTLHDKFLFASFISRDSTFDQ 627
I FYSNILGW+ TV +PFK IVQIE++ TA +FPN I I TLH K+ FASF SRD+T+D
Sbjct: 590 IGFYSNILGWVSTVFIPFKTIVQIEKRATAGIFPNGIVIDTLHTKYTFASFTSRDATYDL 649
Query: 628 IMDIWNQTIINKNFKPKRGTMGSA--DLMNDENLTLDSEASSMDYDDEDVQTNDRTSTSI 685
I ++WNQ I+ K F+ S+ + +DEN D + DD+D+ N +
Sbjct: 650 ITEVWNQIILGKRFRSNSNNTNSSSNSISDDENDDYDDDYDDYGDDDDDLYDNSNNISDS 709
Query: 686 TD 687
TD
Sbjct: 710 TD 711
Score = 146 bits (368), Expect = 2e-34
Identities = 74/201 (36%), Positives = 121/201 (59%), Gaps = 8/201 (3%)
Query: 943 SSLPCLGPKEHEPTECTFEKSSGYEIIDDKISFKSPVGTIFQLICGDDTSYMKRILERQK 1002
+ +P LGPK H PTE ++ + +++++ + + +G + ++ G D SY+ IL+ QK
Sbjct: 736 TGIPPLGPKIHSPTETVYKPAPNEKLVNES-TIHASLGRVVNILFGKDVSYIMAILKAQK 794
Query: 1003 NIEISDIPPF-------QNKTRKYTYVKPLPGSIGPKQAKCYIEEKIESDDVNTCMVLEQ 1055
N +IS IP + K R Y+YVK PG+IGP + KC I E I+ ++ + + Q
Sbjct: 795 NSDISPIPVLVDSPTVSEGKKRDYSYVKTTPGAIGPGKTKCMITETIQHFNLEEYVQVLQ 854
Query: 1056 ISKTPEIPSGNSFEVHTKFYFSWGEKNTTNVLAATNIEWTGKSWIKSAIQKGSINGQKAS 1115
+KTP++PSGNSF V T + SW N T + ++EWTGKS IKS I+KG+ +G +
Sbjct: 855 TTKTPDVPSGNSFYVRTVYLLSWANNNETKLKLYVSVEWTGKSLIKSPIEKGTFDGVTDA 914
Query: 1116 VQDMVAEIKDILSSASSTTKR 1136
+ +V E+ +IL+ +++ KR
Sbjct: 915 TKILVEELGNILTRSATKRKR 935
>SPBC20F10.07 [S] KOG1032 Uncharacterized conserved protein contains GRAM domain
Length = 764
Score = 239 bits (611), Expect = 2e-62
Identities = 156/485 (32%), Positives = 226/485 (46%), Gaps = 38/485 (7%)
Query: 466 PLRNSFSGRNMTRASSISKTSDIVQPPGGPTRLSTSDIPI-KRKTE-------EPELQGI 517
PL + SG N ASS T + PP T S I RK L G
Sbjct: 132 PLERTHSGSNHHEASS---TGHLNLPPLENTLSQGSAITAPSRKVSITSSNGVSARLSGY 188
Query: 518 EYASAKRNTDFHTIFKDTVVSPIERLIADYSCALSKDILIQGRLYISDRHICFYSNILGW 577
+A++KRN DFH IFK V+ P + LI DY CAL +DI + GR+Y+S+ HICF S+I GW
Sbjct: 189 AFANSKRNRDFHRIFK--VLPPEDHLIDDYGCALQRDIFLHGRMYLSESHICFNSSIFGW 246
Query: 578 MKTVVVPFKEIVQIEQKNTAVLFPNAISIQTLHDKFLFASFISRDSTFDQIMDIWNQTII 637
+ +V+P EIV +E+K+TAV+FPNAI I TLH +++FASFISRD+T+ I+ IW
Sbjct: 247 VTNIVIPVTEIVSVEKKSTAVVFPNAIQITTLHARYIFASFISRDTTYQLIIAIW----- 301
Query: 638 NKNFKPKRGTMGSADLMND-----------ENLTLDSEASSMDYDD-EDVQTNDRTSTSI 685
KN P T+ + + D +++ DS A S D+ + ND +S
Sbjct: 302 -KNTHPFLTTLANGHGVMDASGNHHSGSSNQSINADSSAGSEGVDEGTSTEANDESSED- 359
Query: 686 TDSYGNRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXARXXXXXXXXXXXXXXXXXX 745
D N +
Sbjct: 360 DDEDNNTDEANEDAQSNVSDESPKGEGSSHSDNVVLSDGNSVKKMNEDGADTSLLSVSEV 419
Query: 746 XKHEPTTPDYTPKENEREMGETILNAPLGTVINILYGSDVQYFDRILKAQ--GNYDISKF 803
H PT ++T + ++N + TV N+L GSD + K++ I K+
Sbjct: 420 TSHPPT--EWTGSPLAHVLCSDVVNLSVSTVFNLLCGSDTTWIINFFKSEKLTEIKIGKW 477
Query: 804 NEIIQSKKREYVYTKPISGSIGPSKTRCEITESIDKLDLKNYVQVTQVSKTPDIPSGNSF 863
+I R+ Y KP++ +T C IT++I LD+ NY+++ + TPD+PSG SF
Sbjct: 478 EKIDDKWNRKVQYIKPVAPPY--RQTSCYITDTIQHLDINNYIEILSTTSTPDVPSGTSF 535
Query: 864 VVKTIFLFCWADDNSTKVKVYTYVDWSAKSWIKAAVEKGTFDGIKSSTKXXXXXXXXXXX 923
VVKT++ WA + TK+ + V+WS SW+K +EKG +G S K
Sbjct: 536 VVKTLYALSWAHSSKTKLNISYSVEWSKSSWLKGPIEKGAQEGQASYVKDLLTAFENYKV 595
Query: 924 XNKGK 928
KG+
Sbjct: 596 SPKGR 600
Score = 99.8 bits (247), Expect = 3e-20
Identities = 56/187 (29%), Positives = 98/187 (51%), Gaps = 8/187 (4%)
Query: 953 HEPTECTFEKSSGYEIIDDKISFKSPVGTIFQLICGDDTSYMKRILERQK--NIEISDIP 1010
H PTE T + + + D ++ V T+F L+CG DT+++ + +K I+I
Sbjct: 422 HPPTEWTGSPLA-HVLCSDVVNLS--VSTVFNLLCGSDTTWIINFFKSEKLTEIKIGKWE 478
Query: 1011 PFQNK-TRKYTYVKPLPGSIGPKQAKCYIEEKIESDDVNTCMVLEQISKTPEIPSGNSFE 1069
+K RK Y+KP+ +Q CYI + I+ D+N + + + TP++PSG SF
Sbjct: 479 KIDDKWNRKVQYIKPVAPPY--RQTSCYITDTIQHLDINNYIEILSTTSTPDVPSGTSFV 536
Query: 1070 VHTKFYFSWGEKNTTNVLAATNIEWTGKSWIKSAIQKGSINGQKASVQDMVAEIKDILSS 1129
V T + SW + T + + ++EW+ SW+K I+KG+ GQ + V+D++ ++ S
Sbjct: 537 VKTLYALSWAHSSKTKLNISYSVEWSKSSWLKGPIEKGAQEGQASYVKDLLTAFENYKVS 596
Query: 1130 ASSTTKR 1136
K+
Sbjct: 597 PKGRRKK 603
>YFL042c [S] KOG1032 Uncharacterized conserved protein contains GRAM domain
Length = 674
Score = 196 bits (498), Expect = 2e-49
Identities = 134/457 (29%), Positives = 211/457 (45%), Gaps = 46/457 (10%)
Query: 468 RNSFSGRNMTRASSISKTSDIVQPPGGPTRLSTSDIPI-----KRKTEEPELQG-IEYAS 521
R+S N T A S + +P TRL+ S + +R EE + YAS
Sbjct: 134 RSSRRISNATIAEIGSPLQQVEKPDEVKTRLTPSQMKEDNYDHRRFVEERYMDTPYHYAS 193
Query: 522 AKRNTDFHTIFKDTVVSPIERLIADYSCALSKDILIQGRLYISDRHICFYSNILGWMKTV 581
+RN DFH FK V +RL+ D++C L++++L QG+LYI++ H+CF SN+LGW+ V
Sbjct: 194 EQRNKDFHETFKS--VPKDDRLLDDFNCGLNRELLYQGKLYITETHLCFNSNVLGWIAKV 251
Query: 582 VVPFKEIVQIEQKNTAVLFPNAISIQTLHDKFLFASFISRDSTFDQIMDIWNQTIINK-- 639
++ F+++ +E+ + A LFP+AISI+T K LF FISRD+ F + ++W++T++ K
Sbjct: 252 LIAFEDVTFMEKTSAAGLFPSAISIETKMGKTLFNGFISRDAAFGLMKEVWSRTLLQKDM 311
Query: 640 ---NFKPKRGTMGSADLMNDENLTLDSEASSMDYDDEDVQTNDRTSTSITDSYGNRXXXX 696
N K G+ ++D ++D E + D +D D ND + S ++ N
Sbjct: 312 ASENINTKAEKSGNGKEIDDAINSIDEENNDKDANDNDTNENDDENISTNETTPN----- 366
Query: 697 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXARXXXXXXXXXXXXXXXXXXXKHEPTTPDYT 756
+ H + P
Sbjct: 367 ---------------------STSSSPDKEKEKAYKLRADSSYQYDGPIYHHSTSFPAEP 405
Query: 757 PKENEREMGETILNAPLGTVINILYGSDVQYF--DRILKAQGNY--DISKFNEIIQSK-- 810
NE + E + G + I++ S+ F D + +G+ I F I S
Sbjct: 406 MANNEFVLKELPFDCAPGILFEIMFNSEQNEFLLDFLRGQEGSQITTIPNFTSIDGSSMT 465
Query: 811 -KREYVYTKPISGSIGPSKTRCEITESIDKLDLKNYVQVTQVSKTPDIPSGNSFVVKTIF 869
KREY Y K + GP T C + E I + D Y +V +TP++PSG SF KT +
Sbjct: 466 LKREYSYEKALHFPAGPKSTTCYVAEVIKRKDPDTYYEVISSIRTPNVPSGGSFSTKTRY 525
Query: 870 LFCWADDNSTKVKVYTYVDWSAKSWIKAAVEKGTFDG 906
L W D+ + ++V +V+W+ SWIK VE G +G
Sbjct: 526 LIRWNDEITCLLRVSFWVEWTGSSWIKGMVENGCKNG 562
Score = 100 bits (248), Expect = 2e-20
Identities = 62/202 (30%), Positives = 97/202 (47%), Gaps = 11/202 (5%)
Query: 929 SSSPDNRKDIEEVL---SSLPCLGPKEHEPTECTFEKSSGYEIIDDKISFKSPVGTIFQL 985
SSSPD K+ L SS GP H T E + E + ++ F G +F++
Sbjct: 369 SSSPDKEKEKAYKLRADSSYQYDGPIYHHSTSFPAEPMANNEFVLKELPFDCAPGILFEI 428
Query: 986 ICGDDTS-YMKRILERQKNIEISDIPPFQN-------KTRKYTYVKPLPGSIGPKQAKCY 1037
+ + + ++ L Q+ +I+ IP F + R+Y+Y K L GPK CY
Sbjct: 429 MFNSEQNEFLLDFLRGQEGSQITTIPNFTSIDGSSMTLKREYSYEKALHFPAGPKSTTCY 488
Query: 1038 IEEKIESDDVNTCMVLEQISKTPEIPSGNSFEVHTKFYFSWGEKNTTNVLAATNIEWTGK 1097
+ E I+ D +T + +TP +PSG SF T++ W ++ T + + +EWTG
Sbjct: 489 VAEVIKRKDPDTYYEVISSIRTPNVPSGGSFSTKTRYLIRWNDEITCLLRVSFWVEWTGS 548
Query: 1098 SWIKSAIQKGSINGQKASVQDM 1119
SWIK ++ G NGQ + Q M
Sbjct: 549 SWIKGMVENGCKNGQLEAAQLM 570
>YLR072w [S] KOG1032 Uncharacterized conserved protein contains GRAM domain
Length = 693
Score = 158 bits (399), Expect = 6e-38
Identities = 115/430 (26%), Positives = 185/430 (42%), Gaps = 40/430 (9%)
Query: 509 TEEPELQGIEYASAKRNTDFHTIFKDTVVSPIERLIADYSCALSKDILIQGRLYISDRHI 568
T E + +++AS + N F +FK ++P RLI DY C ++ QGR+Y+S+ H+
Sbjct: 147 TNEVKNIEVQFASEEANKKFRQMFKP--LAPNTRLITDYFCYFHREFPYQGRIYLSNTHL 204
Query: 569 CFYSNILGWMKTVVVPFKEIVQIEQKNTAVLFPNAISIQTLHDKFLFASFISRDSTFDQI 628
CF S +L WM + +P EI +++ T +AIS++T+ +++ F+ FI+RD F I
Sbjct: 205 CFNSTVLNWMAKLQIPLNEIKYLDKVTTN---SSAISVETVTNRYTFSGFIARDEVFQLI 261
Query: 629 MDIW---NQTIIN------KNFKPKRGTMG---------SADLMNDENLTLDSEASSM-D 669
+W N T IN + K+G S + ND T +E +S
Sbjct: 262 TRVWSKENLTNINDVLEVDERVSKKKGISSTPSSIFNNVSTNAYNDFISTTTTEPTSRAS 321
Query: 670 YDDEDVQTNDRTSTSITDSYGNRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXARXX 729
Y E+ + S+ D G R
Sbjct: 322 YMSENDMLIEEAIRSVDDYMGTPRASPSSSSSSSSSSSSLGSSTTYY---------CRPV 372
Query: 730 XXXXXXXXXXXXXXXXXKHEPTTPDYTPKENEREMGETILNAPLGTVINILYGSDVQYFD 789
+ P NE + E + P G + +++ D F+
Sbjct: 373 YRLKPNAPFQYEGPFHVQETMDFPYKPEANNEYVLLERQFSVPPGLLFIMMFNEDNPVFE 432
Query: 790 -RILKAQGNYDISKFNEIIQSKK-----REYVYTKPISGSIGPSKTRCEITESIDKLDLK 843
LK Q + +IS + K RE+ YTK + +GP T CE+ E + D +
Sbjct: 433 LSFLKTQDSSNISHIGTFEKVNKDGQHYREFQYTKQLHFPVGPKSTNCEVAEILLHCDWE 492
Query: 844 NYVQVTQVSKTPDIPSGNSFVVKTIFLFCWADDNSTKV-KVYTYVDWSAKSWIKAAVEKG 902
Y+ V +++TP++PSG SF +T ++F W D + K+ +VDW+A SWIK VE
Sbjct: 493 RYINVLSITRTPNVPSGTSFSTRTRYMFRWDDQGQGCILKISFWVDWNASSWIKPMVESN 552
Query: 903 TFDGIKSSTK 912
+G S+TK
Sbjct: 553 CKNGQISATK 562
Score = 79.7 bits (195), Expect = 3e-14
Identities = 48/185 (25%), Positives = 92/185 (48%), Gaps = 10/185 (5%)
Query: 961 EKSSGYEIIDDKISFKSPVGTIFQLICGDDTSYMK-RILERQKNIEISDIPPFQ--NKT- 1016
E ++ Y +++ + F P G +F ++ +D + L+ Q + IS I F+ NK
Sbjct: 400 EANNEYVLLERQ--FSVPPGLLFIMMFNEDNPVFELSFLKTQDSSNISHIGTFEKVNKDG 457
Query: 1017 ---RKYTYVKPLPGSIGPKQAKCYIEEKIESDDVNTCMVLEQISKTPEIPSGNSFEVHTK 1073
R++ Y K L +GPK C + E + D + + I++TP +PSG SF T+
Sbjct: 458 QHYREFQYTKQLHFPVGPKSTNCEVAEILLHCDWERYINVLSITRTPNVPSGTSFSTRTR 517
Query: 1074 FYFSWGEKNTTNVLAATN-IEWTGKSWIKSAIQKGSINGQKASVQDMVAEIKDILSSASS 1132
+ F W ++ +L + ++W SWIK ++ NGQ ++ +D+V +++ +
Sbjct: 518 YMFRWDDQGQGCILKISFWVDWNASSWIKPMVESNCKNGQISATKDLVKLVEEFVEKYVE 577
Query: 1133 TTKRR 1137
+K +
Sbjct: 578 LSKEK 582
>Hs22052111 [S] KOG1032 Uncharacterized conserved protein contains GRAM domain
Length = 650
Score = 111 bits (277), Expect = 9e-24
Identities = 54/122 (44%), Positives = 75/122 (61%), Gaps = 2/122 (1%)
Query: 523 KRNTDFHTIFKDTVVSPIERLIADYSCALSKDILIQGRLYISDRHICFYSNILGWMKTVV 582
+RN DF +F + ERLI DYSCAL ++IL+QGRLY+S+ ICFYSNI W T+
Sbjct: 14 QRNEDFRKLFSK--LPEAERLIVDYSCALQREILLQGRLYLSENWICFYSNIFRWETTIS 71
Query: 583 VPFKEIVQIEQKNTAVLFPNAISIQTLHDKFLFASFISRDSTFDQIMDIWNQTIINKNFK 642
+ KE+ ++++ TA L PNAI I T +K F SF +RD F I +W ++ K
Sbjct: 72 IQLKEVTCLKKEKTAKLIPNAIQICTESEKHFFTSFGARDRCFLLIFRLWQNALLEKTLS 131
Query: 643 PK 644
P+
Sbjct: 132 PR 133
>CE15185 [S] KOG1032 Uncharacterized conserved protein contains GRAM domain
Length = 562
Score = 88.6 bits (218), Expect = 6e-17
Identities = 50/157 (31%), Positives = 84/157 (52%), Gaps = 3/157 (1%)
Query: 523 KRNTDFHTIFKDT-VVSPIERLIADYSCALSKDILIQGRLYISDRHICFYSNILGWMKTV 581
+RN F IF D ++ ++ +A YSCA ++IL QGR++IS ++CFY+NI+GW T+
Sbjct: 71 ERNLQFKKIFVDKGLIEENDQFLASYSCAYQREILAQGRMFISQFNVCFYANIIGWETTL 130
Query: 582 VVPFKEIVQIEQKNTAVLFPNAISIQTLHDKFLFASFISRDSTFDQIMDIWNQTIINKNF 641
V+P KEI +++ A +FPN+I ++ S +T + M + N +
Sbjct: 131 VIPMKEISLVKKMKAAFIFPNSIQNSQNQTPPDGSTPASTKNTSTENMTNLSSRSFNSSI 190
Query: 642 KPKRGTMGSADLMNDENLTLDSEASSMDYDDEDVQTN 678
P+ T S N++ ++S D+ DED T+
Sbjct: 191 SPR--TEASTLKNNNDKDNTSQSSTSSDFHDEDSATH 225
>Hs12965197 [S] KOG1032 Uncharacterized conserved protein contains GRAM domain
Length = 432
Score = 87.8 bits (216), Expect = 1e-16
Identities = 58/201 (28%), Positives = 97/201 (47%), Gaps = 26/201 (12%)
Query: 437 NGRSRRPEVLRDLTMRSLSPAAAT------KLFTAPLRNSFSGRNMTRASSISKTSDIVQ 490
NG + + R T +S +P+ K+ + ++SF G ++ + KT
Sbjct: 33 NGVEEKKKACRSPTAQSPTPSVEADSPDQKKIISLWSKSSFDGASLASDKNDCKTE---- 88
Query: 491 PPGGPTRLSTSDIPIKRKTEEPELQGIEYASAKRNTDFHTIFKDTVVSPIERLIADYSCA 550
S +D +RK Q K N FH +F V E L ++CA
Sbjct: 89 --------SKNDPKTERKKSSSSSQ------YKANMHFHKLFLS--VPTEEPLKQSFTCA 132
Query: 551 LSKDILIQGRLYISDRHICFYSNILGWMKTVVVPFKEIVQIEQKNTAVLFPNAISIQTLH 610
L K+IL QG+L++S+ ICF+S + G + +P + I++ TA+L PNA+ I T+
Sbjct: 133 LQKEILYQGKLFVSENWICFHSKVFGKDTKISIPAFSVTLIKKTKTALLVPNALIIATVT 192
Query: 611 DKFLFASFISRDSTFDQIMDI 631
D+++F S +SRDST+ + +
Sbjct: 193 DRYIFVSLLSRDSTYKLLKSV 213
>Hs20482574 [S] KOG1032 Uncharacterized conserved protein contains GRAM domain
Length = 337
Score = 80.5 bits (197), Expect = 2e-14
Identities = 42/106 (39%), Positives = 64/106 (59%), Gaps = 5/106 (4%)
Query: 523 KRNTDFHTIFKDTVVSPIERLIADY-SCALSKDILIQGRLYISDRHICFYSNILGWMKTV 581
K N +H +FKD P+E ++ SCAL +D L+QGRLYIS +CF++++ G V
Sbjct: 52 KYNQQYHKLFKDV---PLEEVVLKVCSCALQRDFLLQGRLYISPNWLCFHASLFGKDIKV 108
Query: 582 VVPFKEIVQIEQKNTAVLFPNAISIQT-LHDKFLFASFISRDSTFD 626
V+P + I++ A L PN ++I T K++F S +SRDS +D
Sbjct: 109 VIPVVSVQMIKKHKMARLLPNGLAITTNTSQKYIFVSLLSRDSVYD 154
>At1g02120 [S] KOG1032 Uncharacterized conserved protein contains GRAM domain
Length = 556
Score = 72.8 bits (177), Expect = 3e-12
Identities = 34/75 (45%), Positives = 47/75 (62%)
Query: 558 QGRLYISDRHICFYSNILGWMKTVVVPFKEIVQIEQKNTAVLFPNAISIQTLHDKFLFAS 617
+G +Y+ +ICFYSNI G+ ++PF EI +++ TA +FPNAI I K+ FAS
Sbjct: 128 EGHMYLFIHYICFYSNIFGYETKKIIPFAEISCVKRAKTAGIFPNAIEILAGGKKYFFAS 187
Query: 618 FISRDSTFDQIMDIW 632
F+SRD F I D W
Sbjct: 188 FLSRDEAFKLIHDGW 202
>Hs22042713 [R] KOG4347 GTPase-activating protein VRP
Length = 1266
Score = 60.5 bits (145), Expect = 2e-08
Identities = 39/123 (31%), Positives = 69/123 (55%), Gaps = 9/123 (7%)
Query: 528 FHTIFKDTVVSPIERLIADYSCALSKD-ILIQGRLYISDRHICFYSNILGWMKTVVVPFK 586
FH +F + E+L+ YSC+ K + QG +Y+S H+CFYS ++G +V+ +
Sbjct: 148 FHRLFG---MPEEEKLVNYYSCSYWKGKVPRQGWMYLSINHLCFYSFLMGREAKLVIRWV 204
Query: 587 EIVQIEQKNTAVLFPNAISIQTLHDKFLFASFISRDSTF---DQIMDI-WNQTIINKNFK 642
+I Q+E KN +L P+ I + T + F+ F++ + TF +Q+ +I Q + N+ F+
Sbjct: 205 DITQLE-KNATLLLPDVIKVSTRSSEHFFSVFLNINETFKLMEQLANIAMRQLLDNEGFE 263
Query: 643 PKR 645
R
Sbjct: 264 QDR 266
>At3g59660 [S] KOG1032 Uncharacterized conserved protein contains GRAM domain
Length = 604
Score = 55.1 bits (131), Expect = 7e-07
Identities = 29/68 (42%), Positives = 41/68 (59%), Gaps = 1/68 (1%)
Query: 539 PIERLIADYSCALSKDILIQGRLYISDRHICFYSNILGWMKTVVVPFKEIVQIEQKNTAV 598
P E + YSCAL + L GR+Y+S HICF+SN+ VVVP +I +I + A+
Sbjct: 239 PDEVVEHSYSCALERSFLYHGRMYVSAWHICFHSNVFSKQMKVVVPLGDIDEIRRSQHAL 298
Query: 599 LFPNAISI 606
+ P AI+I
Sbjct: 299 INP-AITI 305
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.312 0.130 0.368
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 67,908,741
Number of Sequences: 60738
Number of extensions: 2758452
Number of successful extensions: 5562
Number of sequences better than 1.0e-05: 12
Number of HSP's better than 0.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 5497
Number of HSP's gapped (non-prelim): 39
length of query: 1336
length of database: 30,389,216
effective HSP length: 119
effective length of query: 1217
effective length of database: 23,161,394
effective search space: 28187416498
effective search space used: 28187416498
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)