ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV4447 good R KOG2647 General function prediction only Predicted Dolichyl-phosphate-mannose-protein mannosyltransferase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV4447 1526177 1527427 417
(417 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YBR004c [R] KOG2647 Predicted Dolichyl-phosphate-mannose-protein... 358 1e-98
SPAC18B11.05 [R] KOG2647 Predicted Dolichyl-phosphate-mannose-pr... 128 2e-29
HsM8923442 [R] KOG2647 Predicted Dolichyl-phosphate-mannose-prot... 67 4e-11
Hs21361771 [R] KOG2647 Predicted Dolichyl-phosphate-mannose-prot... 67 4e-11
7302855 [R] KOG2647 Predicted Dolichyl-phosphate-mannose-protein... 67 4e-11
CE13457 [R] KOG2647 Predicted Dolichyl-phosphate-mannose-protein... 50 6e-06
>YBR004c [R] KOG2647 Predicted Dolichyl-phosphate-mannose-protein
mannosyltransferase
Length = 433
Score = 358 bits (918), Expect = 1e-98
Identities = 191/442 (43%), Positives = 272/442 (61%), Gaps = 44/442 (9%)
Query: 11 LIINVTFFAV--KLLQFGLVWLAP-RTFDTSTHLFLEHYGITSNP----------WWGKL 57
+I+ +T + V + +Q+ LV+L P R FDTST L L + S+P +W KL
Sbjct: 1 MIVGLTLYFVLFRSIQYLLVFLTPIRQFDTSTSLLLNE--LCSSPSEINSYWNKYFWNKL 58
Query: 58 LSWDSIFFLKTSLWMRNSGYSAPQYEHEWAFSPIWSIIIQ------SSDLQHIVLKAVSF 111
LSWDS+FF+K S PQ+EHE+AFS +W+ ++ + + H + V+
Sbjct: 59 LSWDSVFFIKNI----TSKNGKPQFEHEYAFSQLWTFFVRLFIKSNNDSIYHALRVGVAI 114
Query: 112 NLLLHYLSTWIVYALTKLTFPPFGQNVQTK-----LALTTSVLFILSSAAGFLISVYSEP 166
+L YLS ++Y LTK F QN++ +A TS+LF L+SAAGFL S+YSEP
Sbjct: 115 ENVLFYLSGIVLYFLTKKIF---SQNIRQSQFARTIAKKTSLLFFLTSAAGFLTSIYSEP 171
Query: 167 IAFTFSLLGMLFRQWSISFDVYGNLHMDKLKWISYXXXXXX-XXXXXXNRSNCLLLGLFY 225
++F F+ +G+ R+ +IS V G + W Y NRSNC+LLG+++
Sbjct: 172 LSFFFAFVGIWSRECTISVPVLGQFDISWRYWFPYSFISMACFTLASLNRSNCVLLGIYF 231
Query: 226 VHDCLNLFKQKKWITSIFYPILSGTILFGVFVYFHYYFPYAALCPERGEWCNSKIYG-LP 284
+ D + L K +K++ +I +P+LSG+++F +Y YY PY CP+RGEWC S+++ +
Sbjct: 232 IFDLIELTKNRKFVKAICFPLLSGSLMFSALLYQQYYLPYKTFCPQRGEWCKSQLFSSIF 291
Query: 285 IPYQSLYSYIQSKHWNVGFLKYWTPNNIPNFLFGLPNIVITWNAITYFSYQYPSRNMKPY 344
I SLYSYIQS +W VG LKYWTPNNIPNFLF +PNI+I + YFS YPS N+K
Sbjct: 292 ITKTSLYSYIQSHYWGVGLLKYWTPNNIPNFLFAVPNIIILIYSSIYFSKIYPSYNLKAL 351
Query: 345 IWIARIFLFIMVFLANVQIINRVSSFLPLSLWYISDSLIK--NPHEM-------RIVKYY 395
+WI R + I+ F A+VQI+NR++SFLPL LWY++D L+K +P +M +IVK+Y
Sbjct: 352 VWITRALVVIVCFFAHVQILNRIASFLPLHLWYLADRLVKTSDPKKMENPKGDDKIVKFY 411
Query: 396 VMWLLIWIPTQTALFACFLPPA 417
+ WL WIP QT LFA FLPPA
Sbjct: 412 IYWLAFWIPLQTILFAAFLPPA 433
>SPAC18B11.05 [R] KOG2647 Predicted Dolichyl-phosphate-mannose-protein
mannosyltransferase
Length = 426
Score = 128 bits (321), Expect = 2e-29
Identities = 102/374 (27%), Positives = 180/374 (47%), Gaps = 49/374 (13%)
Query: 58 LSWDSIFFLKTSLWMRNSGYSAPQYEHEWAFSPIWSIII-----QSSDLQHIVLKAVSFN 112
+ WD+I+F+ ++ + +E EWAFS +W II +S D+ + + + +
Sbjct: 88 IRWDAIYFVDMAV-------NGSLFEQEWAFSSLWPKIISFLAFRSKDVVLLGIVSCFAS 140
Query: 113 LLLHYLSTWIVYALTKLTFPPFGQNVQTKLALTTSVLFILSSAAGFLISV-YSEPIAFTF 171
+ H ++ + +Y LTK F + Q A T V+F S +G +SV Y+E + F
Sbjct: 141 IFFHAIACYALYLLTKSIF-----SNQKMTAYT--VIFYCFSPSGIYMSVGYTESLFAAF 193
Query: 172 SLLGMLFRQWSISFDVYGNLHMDKLKWISYXXXXXXXXXXXXNRSNCLLLGLFYVHDC-L 230
S LG+L L + K ++ + C+ G+ + +
Sbjct: 194 SFLGLL-------------LFIKKQQYPAAFLWSLATLIRSNGIFWCIFFGMPAIGTLKI 240
Query: 231 NLFKQKKWITSIFYPILSGTILFGVFVYFHY--YFPYAALCPERGEWCNSKIYGLPIPYQ 288
+L + + + + GT + V F Y Y + CP WCN LP+
Sbjct: 241 SLERLQLTFMQVSQLVGYGTKCLIILVPFFYNQYLGFKLFCPGVA-WCNKS---LPL--- 293
Query: 289 SLYSYIQSKHWNVGFLKYWTPNNIPNFLFGLPNIVITWNAITYF---SYQYPSRNMKPYI 345
+Y +Q K+WNVGFL+YWT NNIPNFLF L +I+ A+ Y S + R++K ++
Sbjct: 294 -IYPAVQEKYWNVGFLRYWTLNNIPNFLFALLSIIPILFALFYSISGSTLHSFRSIKSHL 352
Query: 346 WIARIFLFIMVFLANVQIINRVSSFLPLSLWYISD-SLIKNPHEMRIVKYYVMWL-LIWI 403
++ ++L+I F + Q++NR+SS LPL W ++ +L ++ + ++++ +++
Sbjct: 353 VLSALYLYIGCFHMHTQVLNRMSSALPLLYWSMAHATLYAKSRNLKAFGHCILFVWIVYT 412
Query: 404 PTQTALFACFLPPA 417
Q L+ FLPPA
Sbjct: 413 VIQAGLYGSFLPPA 426
>HsM8923442 [R] KOG2647 Predicted Dolichyl-phosphate-mannose-protein
mannosyltransferase
Length = 493
Score = 67.4 bits (163), Expect = 4e-11
Identities = 85/322 (26%), Positives = 129/322 (39%), Gaps = 82/322 (25%)
Query: 55 GKLLSWDSIFFLKTSLWMRNSGYSAPQYEHEWAFSPIWSI-IIQSSDLQH---------- 103
G L WD+ FL ++ GY YEH +AF P + + ++ ++L
Sbjct: 61 GGLSHWDAEHFL----FIAEHGYL---YEHNFAFFPGFPLALLVGTELLRPLRGLLSLRS 113
Query: 104 -IVLKAVSFNLLLHYLSTWIVYALTKLTFPPFGQNVQTKLALTTSVLFILSSAAGFLISV 162
+++ S N L L+ ++ L L Q+ L LF LS A FL +
Sbjct: 114 CLLISVASLNFLFFMLAAVALHDLGCLVLHCPHQSFYAAL------LFCLSPANVFLAAG 167
Query: 163 YSEPIAFTFSLLGMLFRQWSISFDVYGNLHMDKLKWISYXXXXXXXXXXXXNRSNCLLLG 222
YSE + F+LL +F G L ++ W S RSN L+
Sbjct: 168 YSEAL---FALL---------TFSAMGQLERGRV-WTSVLLFAFATGV----RSNGLVSV 210
Query: 223 LFYVHD----------CLNLFKQK-KWITSIFYPILSGTILFGVFVYFHYYF-------- 263
F +H LN +Q K + S+F + + + F +F Y+ Y
Sbjct: 211 GFLMHSQCQGFFSSLTMLNPLRQLFKLMASLFLSVFTLGLPFALFQYYAYTQFCLPGSAR 270
Query: 264 ----PYAALCPERG---------EWCNSKIYGLPIPYQSLYSYIQSKHWNVGFLKYWTPN 310
P L ++G WC + +P+ +YSYIQ +WNVGFLKY+
Sbjct: 271 PIPEPLVQLAVDKGYRIAEGNEPPWC---FWDVPL----IYSYIQDVYWNVGFLKYYELK 323
Query: 311 NIPNFLFGLP-NIVITWNAITY 331
+PNFL P I++ W TY
Sbjct: 324 QVPNFLLAAPVAILVAWATWTY 345
>Hs21361771 [R] KOG2647 Predicted Dolichyl-phosphate-mannose-protein
mannosyltransferase
Length = 493
Score = 67.4 bits (163), Expect = 4e-11
Identities = 85/322 (26%), Positives = 129/322 (39%), Gaps = 82/322 (25%)
Query: 55 GKLLSWDSIFFLKTSLWMRNSGYSAPQYEHEWAFSPIWSI-IIQSSDLQH---------- 103
G L WD+ FL ++ GY YEH +AF P + + ++ ++L
Sbjct: 61 GGLSHWDAEHFL----FIAEHGYL---YEHNFAFFPGFPLALLVGTELLRPLRGLLSLRS 113
Query: 104 -IVLKAVSFNLLLHYLSTWIVYALTKLTFPPFGQNVQTKLALTTSVLFILSSAAGFLISV 162
+++ S N L L+ ++ L L Q+ L LF LS A FL +
Sbjct: 114 CLLISVASLNFLFFMLAAVALHDLGCLVLHCPHQSFYAAL------LFCLSPANVFLAAG 167
Query: 163 YSEPIAFTFSLLGMLFRQWSISFDVYGNLHMDKLKWISYXXXXXXXXXXXXNRSNCLLLG 222
YSE + F+LL +F G L ++ W S RSN L+
Sbjct: 168 YSEAL---FALL---------TFSAMGQLERGRV-WTSVLLFAFATGV----RSNGLVSV 210
Query: 223 LFYVHD----------CLNLFKQK-KWITSIFYPILSGTILFGVFVYFHYYF-------- 263
F +H LN +Q K + S+F + + + F +F Y+ Y
Sbjct: 211 GFLMHSQCQGFFSSLTMLNPLRQLFKLMASLFLSVFTLGLPFALFQYYAYTQFCLPGSAR 270
Query: 264 ----PYAALCPERG---------EWCNSKIYGLPIPYQSLYSYIQSKHWNVGFLKYWTPN 310
P L ++G WC + +P+ +YSYIQ +WNVGFLKY+
Sbjct: 271 PIPEPLVQLAVDKGYRIAEGNEPPWC---FWDVPL----IYSYIQDVYWNVGFLKYYELK 323
Query: 311 NIPNFLFGLP-NIVITWNAITY 331
+PNFL P I++ W TY
Sbjct: 324 QVPNFLLAAPVAILVAWATWTY 345
>7302855 [R] KOG2647 Predicted Dolichyl-phosphate-mannose-protein
mannosyltransferase
Length = 449
Score = 67.4 bits (163), Expect = 4e-11
Identities = 91/416 (21%), Positives = 154/416 (36%), Gaps = 104/416 (25%)
Query: 55 GKLLSWDSIFFLKTSLWMRNSGYSAPQYEHEWAFSPIWSIIIQS------------SDLQ 102
G L WD +FL + + YS YE+ AF P++ ++++ S
Sbjct: 57 GGLRHWDGEYFLHIA----ENLYS---YENTLAFYPLYPVVVRHVGQAVEAIGISLSQES 109
Query: 103 HIVLKAVSFNLLLHYLSTWIVYALTKLTFPPFGQNVQTKLALTTSVLFILSSAAGFLISV 162
+++ AV+ N L S +++ LT++ F ++ L ++ + A F +
Sbjct: 110 ILLVVAVALNFWLFCESANLLFQLTQVLFNDLNKSWNAAL------IYCFNPATIFFTAA 163
Query: 163 YSEPIAFTFSLLGMLFRQWSISFDVYGNLHM-----DKLKWISYXXXXXXXXXXXXNRSN 217
YSE +F Y +LH+ Y RSN
Sbjct: 164 YSE------------------TFFAYSSLHLMLECSKPTGSFRYLRLGTALAACLLCRSN 205
Query: 218 CLL-LGL-FYVHDCLNLFKQKKWITSIFYPILSGTILFGVFVYFHYYFP-YAALC----- 269
L+ LG Y L K K+ T + ++ TIL + + YYF Y C
Sbjct: 206 GLITLGYPLYFFGRQLLLKNKEPNTCMQLTQMTLTILGAIGILHTYYFYIYRLYCLPNTR 265
Query: 270 ----------------------PERGEWCNSKIYGLPIPYQSLYSYIQSKHWNVGFLKYW 307
E WC Y LP PY +Y+QS +W+VGFL+Y+
Sbjct: 266 PNHPQHIVDYAVERKYLLSGQGSEGSPWCQ---YTLPFPY----TYVQSHYWDVGFLRYY 318
Query: 308 TPNNIPNFLFGLPNI-VITWNAITY----------------FSYQYPSRNMKPYIWIARI 350
+PNFL LP + + W+ Y F P++ A I
Sbjct: 319 KWKQLPNFLLALPMLSFMHWHCYDYMQHLAKAVWAKLTPSGFKELIRDHTTFPFVLHAAI 378
Query: 351 FLFIMVFLANVQIINR-VSSFLPLSLWYISDSLIKNPHEMRIVKYYVMWLLIWIPT 405
+ ++Q+ R ++S P+ W+ +D + K ++++ + L +W T
Sbjct: 379 LTLVCTVYVHIQVSTRLLASATPVFYWFAADHMPKTLAQLKL-RSKAGALFVWCTT 433
>CE13457 [R] KOG2647 Predicted Dolichyl-phosphate-mannose-protein
mannosyltransferase
Length = 672
Score = 50.1 bits (118), Expect = 6e-06
Identities = 32/114 (28%), Positives = 51/114 (44%), Gaps = 22/114 (19%)
Query: 271 ERGEWCNS-KIYGLPIPYQSLYSYIQSKHWNVGFLKYWTPNNIPNFLFGLPNIVIT---- 325
E+ WC K++G+ Y Y IQ+K+W+V F YW IP FL LP ++T
Sbjct: 485 EKTTWCKKPKLFGIIARY---YGEIQTKYWSVKFFGYWKIKKIPCFLMMLPAAILTVLAI 541
Query: 326 ------------WNAITYFSYQYPSRNMKPYIWIARIFLFIMVFLANVQIINRV 367
WN I + + S + P + + LF+ +F N ++ R+
Sbjct: 542 KSSWNDVFLNKRWNNIWVLTAR--SDHSLPMAIHSSVLLFVAIFYINSEVFTRI 593
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.329 0.143 0.481
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 25,815,960
Number of Sequences: 60738
Number of extensions: 1065716
Number of successful extensions: 2779
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 2762
Number of HSP's gapped (non-prelim): 10
length of query: 417
length of database: 30,389,216
effective HSP length: 109
effective length of query: 308
effective length of database: 23,768,774
effective search space: 7320782392
effective search space used: 7320782392
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)