ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV4596 good J KOG2971 Translation, ribosomal structure and biogenesis RNA-binding protein required for biogenesis of the ribosomal 60S subunit
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV4596 1578734 1579627 298
(298 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YOL077c [J] KOG2971 RNA-binding protein required for biogenesis ... 449 e-126
SPBC800.06 [J] KOG2971 RNA-binding protein required for biogenes... 294 1e-79
Hs20545215 [J] KOG2971 RNA-binding protein required for biogenes... 253 3e-67
Hs19311012 [J] KOG2971 RNA-binding protein required for biogenes... 251 1e-66
At1g52930 [J] KOG2971 RNA-binding protein required for biogenesi... 238 6e-63
At3g15460 [J] KOG2971 RNA-binding protein required for biogenesi... 231 1e-60
7292473 [J] KOG2971 RNA-binding protein required for biogenesis ... 230 2e-60
CE00268 [J] KOG2971 RNA-binding protein required for biogenesis ... 211 8e-55
ECU01g0360 [J] KOG2971 RNA-binding protein required for biogenes... 90 4e-18
>YOL077c [J] KOG2971 RNA-binding protein required for biogenesis of the
ribosomal 60S subunit
Length = 291
Score = 449 bits (1154), Expect = e-126
Identities = 216/268 (80%), Positives = 234/268 (86%), Gaps = 1/268 (0%)
Query: 30 KQFMNKQRTLLITSRGVTYRHRHLVQDLNSLLPHSRKEPKLDTKKDLGQLNEIAELYNCN 89
KQFMNKQRTLLI+SRGV YRHRHL+QDL+ LLPHSRKEPKLDTKKDL QLNEIAELYNCN
Sbjct: 25 KQFMNKQRTLLISSRGVNYRHRHLIQDLSGLLPHSRKEPKLDTKKDLQQLNEIAELYNCN 84
Query: 90 NIIFFEARKHQDLYLWLSKPPNGPTIKFYLQNLHTMDELNFTGNCLKGSRPILSFDGRFD 149
N++FFEARKHQDLYLWLSKPPNGPTIKFY+QNLHTMDELNFTGNCLKGSRP+LSFD RF+
Sbjct: 85 NVLFFEARKHQDLYLWLSKPPNGPTIKFYIQNLHTMDELNFTGNCLKGSRPVLSFDQRFE 144
Query: 150 SEPHYRLIKELFIHNFGVPPQARKSKPFIDHIMSFSIVDEKIWVRTYEISHEARNNVEYE 209
S PHY+LIKEL +HNF VPP ARKSKPFIDH+MSFSIVD+KIWVRTYEISH +N EYE
Sbjct: 145 SSPHYQLIKELLVHNFCVPPNARKSKPFIDHVMSFSIVDDKIWVRTYEISHSTKNKEEYE 204
Query: 210 DKEEDETSLVEIGPRFVMTPILILEGSFGGPKIYENRQYVSPNFVRSQIKQQXXXXXXXX 269
D EED SLVEIGPRFVMT ILILEGSFGGPKIYEN+QYVSPN VR+QIKQQ
Sbjct: 205 DGEED-ISLVEIGPRFVMTVILILEGSFGGPKIYENKQYVSPNVVRAQIKQQAAEEAKSR 263
Query: 270 XXXXXXXXLKKRESVLAVDPLSNDALFK 297
+K+RE+VLA DPLSNDALFK
Sbjct: 264 AEAAVERKIKRRENVLAADPLSNDALFK 291
>SPBC800.06 [J] KOG2971 RNA-binding protein required for biogenesis of the
ribosomal 60S subunit
Length = 295
Score = 294 bits (752), Expect = 1e-79
Identities = 142/258 (55%), Positives = 185/258 (71%), Gaps = 7/258 (2%)
Query: 35 KQRTLLITSRGVTYRHRHLVQDLNSLLPHSRKEPKLDTKKDLGQLNEIAELYNCNNIIFF 94
KQ+ L+++SRGVTYR RHL+ DL S++PHS+K+ KLD+K L QLNE+AELYNCNNI FF
Sbjct: 42 KQKVLVLSSRGVTYRQRHLLNDLVSMMPHSKKDSKLDSKDRLYQLNELAELYNCNNIFFF 101
Query: 95 EARKHQDLYLWLSKPPNGPTIKFYLQNLHTMDELNFTGNCLKGSRPILSFDGRFDSEPHY 154
E+R+ +DLYL +++ PNGPT+KF+++NLHTMDELN TGN LKGSRPILSFD FD+ PH
Sbjct: 102 ESRRREDLYLHIARAPNGPTVKFHVENLHTMDELNMTGNALKGSRPILSFDKTFDTAPHL 161
Query: 155 RLIKELFIHNFGVPPQARKSKPFIDHIMSFSIVDEKIWVRTYEISHEARNNVEYEDKEED 214
+++KEL FG+P AR+SKPFID + + +I D KIW R YEI E EDK +D
Sbjct: 162 KVVKELLQQTFGIPKGARRSKPFIDRVCTLTIADGKIWFRNYEIR-------ENEDKSKD 214
Query: 215 ETSLVEIGPRFVMTPILILEGSFGGPKIYENRQYVSPNFVRSQIKQQXXXXXXXXXXXXX 274
+L+EIGPRFVMT I ILEGSFGGP IY+N +VS VR+ I+ Q
Sbjct: 215 PVTLIEIGPRFVMTIINILEGSFGGPVIYKNDTFVSSTMVRAAIRNQAAQRYVNRQESKL 274
Query: 275 XXXLKKRESVLAVDPLSN 292
++ +++V+ DPL N
Sbjct: 275 ERQVRAQQNVIPEDPLDN 292
>Hs20545215 [J] KOG2971 RNA-binding protein required for biogenesis of the
ribosomal 60S subunit
Length = 353
Score = 253 bits (645), Expect = 3e-67
Identities = 123/264 (46%), Positives = 170/264 (63%), Gaps = 15/264 (5%)
Query: 31 QFMNKQRTLLITSRGVTYRHRHLVQDLNSLLPHSRKEPKLDTKKDLGQLNEIAELYNCNN 90
++ NK+R L+ +SRG+ +R RHL+QDL L+PHS+ + K+D K L +NE+ E+ NCN
Sbjct: 55 KWKNKERILIFSSRGINFRTRHLMQDLRMLMPHSKADTKMDRKDKLFVINEVCEMKNCNK 114
Query: 91 IIFFEARKHQDLYLWLSKPPNGPTIKFYLQNLHTMDELNFTGNCLKGSRPILSFDGRFDS 150
I+FEA+K QDLY+WLS P+GP+ KF +QN+HT+ EL TGNCLKGSRP+LSFD FD
Sbjct: 115 CIYFEAKKKQDLYMWLSNSPHGPSAKFLVQNIHTLAELKMTGNCLKGSRPLLSFDPAFDE 174
Query: 151 EPHYRLIKELFIHNFGVPPQARKSKPFIDHIMSFSIVDEKIWVRTYEISHEARNNVEYED 210
PHY L+KEL I F P KS+PF+DH+ +F+I+D +IW R ++I
Sbjct: 175 LPHYALLKELLIQIFSTPRYHPKSQPFVDHVFTFTILDNRIWFRNFQII----------- 223
Query: 211 KEEDETSLVEIGPRFVMTPILILEGSFGGPKIYENRQYVSPNFVRSQIKQQXXXXXXXXX 270
E++ +LVEIGPRFV+ I I +GSFGGP +YEN Y SPN R I+
Sbjct: 224 --EEDAALVEIGPRFVLNLIKIFQGSFGGPTLYENPHYQSPNMHRRVIRSITAAKYREKQ 281
Query: 271 XXXXXXXLKKRE--SVLAVDPLSN 292
L+K+E ++L DP ++
Sbjct: 282 QVKDVQKLRKKEPKTLLPHDPTAD 305
>Hs19311012 [J] KOG2971 RNA-binding protein required for biogenesis of the
ribosomal 60S subunit
Length = 354
Score = 251 bits (640), Expect = 1e-66
Identities = 117/229 (51%), Positives = 158/229 (68%), Gaps = 13/229 (5%)
Query: 31 QFMNKQRTLLITSRGVTYRHRHLVQDLNSLLPHSRKEPKLDTKKDLGQLNEIAELYNCNN 90
++ NK+R L+ +SRG+ +R RHL+QDL L+PHS+ + K+D K L +NE+ E+ NCN
Sbjct: 55 KWKNKERILIFSSRGINFRTRHLMQDLRMLMPHSKADTKMDRKDKLFVINEVCEMKNCNK 114
Query: 91 IIFFEARKHQDLYLWLSKPPNGPTIKFYLQNLHTMDELNFTGNCLKGSRPILSFDGRFDS 150
I+FEA+K QDLY+WLS P+GP+ KF +QN+HT+ EL TGNCLKGSRP+LSFD FD
Sbjct: 115 CIYFEAKKKQDLYMWLSNSPHGPSAKFLVQNIHTLAELKMTGNCLKGSRPLLSFDPAFDE 174
Query: 151 EPHYRLIKELFIHNFGVPPQARKSKPFIDHIMSFSIVDEKIWVRTYEISHEARNNVEYED 210
PHY L+KEL I F P KS+PF+DH+ +F+I+D +IW R ++I
Sbjct: 175 LPHYALLKELLIQIFSTPRYHPKSQPFVDHVFTFTILDNRIWFRNFQII----------- 223
Query: 211 KEEDETSLVEIGPRFVMTPILILEGSFGGPKIYENRQYVSPNFVRSQIK 259
E++ +LVEIGPRFV+ I I +GSFGGP +YEN Y SPN R I+
Sbjct: 224 --EEDAALVEIGPRFVLNLIKIFQGSFGGPTLYENPHYQSPNMHRRVIR 270
>At1g52930 [J] KOG2971 RNA-binding protein required for biogenesis of the
ribosomal 60S subunit
Length = 320
Score = 238 bits (608), Expect = 6e-63
Identities = 110/230 (47%), Positives = 160/230 (68%), Gaps = 1/230 (0%)
Query: 32 FMNKQRTLLITSRGVTYRHRHLVQDLNSLLPHSRKEPKLDTKKDLGQ-LNEIAELYNCNN 90
F NK++ L+ SR +++R+R L+ ++ SLLPH +K+ K++ K G LNE+ EL N N+
Sbjct: 53 FKNKEKVLVTCSRRISFRYRSLMLNIVSLLPHCKKDSKVEAKSSKGATLNELIELKNSNS 112
Query: 91 IIFFEARKHQDLYLWLSKPPNGPTIKFYLQNLHTMDELNFTGNCLKGSRPILSFDGRFDS 150
+FFE RKH+DLY+W+ K PNGP++KF ++ +H M+E+ TGN LKGSRP+L+F FD
Sbjct: 113 CLFFECRKHKDLYMWMVKSPNGPSVKFLVKAVHAMEEMKLTGNHLKGSRPLLTFSSNFDK 172
Query: 151 EPHYRLIKELFIHNFGVPPQARKSKPFIDHIMSFSIVDEKIWVRTYEISHEARNNVEYED 210
+ H++L+KE+ FG+P + RKSKP+ DH+ FSIVDE IW R Y+IS + +
Sbjct: 173 DAHWKLLKEMLTQVFGIPKEHRKSKPYHDHVFVFSIVDEHIWFRNYQISVPHNESDKIAK 232
Query: 211 KEEDETSLVEIGPRFVMTPILILEGSFGGPKIYENRQYVSPNFVRSQIKQ 260
D+ +L+E+GPRF + PI I GSFGGP +YEN YVSPN +R+ K+
Sbjct: 233 GGLDKMTLIEVGPRFCLNPIKIFAGSFGGPTLYENPLYVSPNQIRALEKR 282
>At3g15460 [J] KOG2971 RNA-binding protein required for biogenesis of the
ribosomal 60S subunit
Length = 315
Score = 231 bits (588), Expect = 1e-60
Identities = 107/230 (46%), Positives = 158/230 (68%), Gaps = 1/230 (0%)
Query: 32 FMNKQRTLLITSRGVTYRHRHLVQDLNSLLPHSRKEPKLDTKKDLGQ-LNEIAELYNCNN 90
F NK++ L+ SR + +R+RHL+ ++ SLLPH +K+ K++ K G LNE+ EL ++
Sbjct: 49 FRNKEKVLVTCSRRINFRYRHLMLNMVSLLPHCKKDSKVEAKSSRGATLNELIELKGSSS 108
Query: 91 IIFFEARKHQDLYLWLSKPPNGPTIKFYLQNLHTMDELNFTGNCLKGSRPILSFDGRFDS 150
+FFE RKH+DLY+W+ K P GP++KF + +HTM+EL TGN LKGSRP+L+F F++
Sbjct: 109 CLFFECRKHKDLYMWMVKSPGGPSVKFLVNAVHTMEELKLTGNHLKGSRPLLTFSSNFEN 168
Query: 151 EPHYRLIKELFIHNFGVPPQARKSKPFIDHIMSFSIVDEKIWVRTYEISHEARNNVEYED 210
+ H++L+KE+ FG+P RKSKP+ DH+ FSIVD+ IW R Y+IS + +
Sbjct: 169 DAHWKLLKEMLTQIFGIPEGHRKSKPYHDHVFVFSIVDDHIWFRNYQISVPHNESDKIAR 228
Query: 211 KEEDETSLVEIGPRFVMTPILILEGSFGGPKIYENRQYVSPNFVRSQIKQ 260
+ D+ +L+E+GPRF + PI I GSFGG +YEN YVSPN +R+ K+
Sbjct: 229 GDLDKMTLIEVGPRFCLNPIKIFGGSFGGTTLYENPFYVSPNQIRALEKR 278
>7292473 [J] KOG2971 RNA-binding protein required for biogenesis of the
ribosomal 60S subunit
Length = 356
Score = 230 bits (587), Expect = 2e-60
Identities = 109/272 (40%), Positives = 170/272 (62%), Gaps = 17/272 (6%)
Query: 29 EKQFMNKQRTLLITSRGVTYRHRHLVQDLNSLLPHSRKEPKLDTKKDLGQLNEIAELYNC 88
+++++NKQR L+ ++RG+++R RHL++D+ +L+PH R E K++ K L +NE+ E+ +C
Sbjct: 40 KEKWVNKQRVLVFSARGISHRDRHLMKDIKTLMPHHRPESKMERSKTLSVVNEMCEMKHC 99
Query: 89 NNIIFFEARKHQDLYLWLSKPPN--GPTIKFYLQNLHTMDELNFTGNCLKGSRPILSFDG 146
N + FE R+ +DLY+W+S GP+ KF ++N+HTM EL TGNCL+GSRP+LSFD
Sbjct: 100 NKAMLFEGRRKRDLYMWISNTSGSTGPSAKFLIENIHTMAELKMTGNCLRGSRPLLSFDS 159
Query: 147 RFDSEPHYRLIKELFIHNFGVPPQARKSKPFIDHIMSFSIVDEKIWVRTYEISHEARNNV 206
+FD PH +L+KELF+ + VP KS+PF+DH+ +F+ +D++IW R ++I E
Sbjct: 160 KFDELPHLKLLKELFVQTYSVPNHHPKSQPFVDHVFTFTYLDKRIWFRNFQILSE----- 214
Query: 207 EYEDKEEDETSLVEIGPRFVMTPILILEGSFGGPKIYENRQYVSPNFVRSQIKQQXXXXX 266
+ L E+GPR+VM P+ I +GSF G I+EN YVSP+ R +K+
Sbjct: 215 --------DGGLSEVGPRYVMNPVKIFDGSFTGKTIWENPDYVSPSKQRQVLKKAAKDKY 266
Query: 267 XXXXXXXXXXXLKKRESVLAVDPLSNDALFKD 298
+ + A D + ND LF+D
Sbjct: 267 VNRVEQKVKH--EATRPIRAYDGMDNDELFED 296
>CE00268 [J] KOG2971 RNA-binding protein required for biogenesis of the
ribosomal 60S subunit
Length = 352
Score = 211 bits (538), Expect = 8e-55
Identities = 98/225 (43%), Positives = 146/225 (64%), Gaps = 14/225 (6%)
Query: 34 NKQRTLLITSRGVTYRHRHLVQDLNSLLPHSRKEPKLDTKKDLGQLNEIAELYNCNNIIF 93
N++R L++ SRG R R+L++D+ LLPH++ + KLD +K L LNEIAE+ NC +++
Sbjct: 61 NRERVLVLCSRGADVRTRYLMKDIKDLLPHAKGDSKLDQQKSLNVLNEIAEMKNCTKVMY 120
Query: 94 FEARKHQDLYLWLSKPPNGPTIKFYLQNLHTMDELNFTGNCLKGSRPILSFDGRFDSEPH 153
FE+RK +D YLW+S GP+IKF + N+HTM EL +GNCL+ SRP+LSFD FD +P
Sbjct: 121 FESRKRKDTYLWMSNVEKGPSIKFLVHNVHTMKELKMSGNCLRASRPVLSFDDAFDKKPQ 180
Query: 154 YRLIKELFIHNFGVPPQARKSKPFIDHIMSFSIVD-EKIWVRTYEISHEARNNVEYEDKE 212
+LIK + + G P +S+PF+DH+ +FS+ + +KIW R ++I E+
Sbjct: 181 LKLIKAVLMQTLGTPHHHPRSQPFVDHVFNFSVGEGDKIWFRNFQIVDES---------- 230
Query: 213 EDETSLVEIGPRFVMTPILILEGSFGGPKIYENRQYVSPNFVRSQ 257
L E+GPRFV+ + + GSF G +Y+N YVSPN +R +
Sbjct: 231 ---LQLQEVGPRFVLEMVRLFAGSFEGAVLYDNPNYVSPNVIRRE 272
>ECU01g0360 [J] KOG2971 RNA-binding protein required for biogenesis of the
ribosomal 60S subunit
Length = 196
Score = 89.7 bits (221), Expect = 4e-18
Identities = 58/207 (28%), Positives = 103/207 (49%), Gaps = 26/207 (12%)
Query: 38 TLLITSRGVTYRHRHLVQDLNSLLPHSRKEPKLDTKKDLGQLNEIAELYNCNNIIFFEAR 97
++++++RG + + RHL++D++ L+ +E K D K+ G+L + + C++++FF +
Sbjct: 3 SVILSTRGASVKIRHLMKDISRLVK-VEEEQKWDMGKNYGELRSLMTINECDSMLFFRST 61
Query: 98 KHQDLYLWLSKPPNGPTIKFYLQNLHTMDELNFTGNCLKGSRPILSFDGRFDSEPHYRLI 157
K D LW+ +G ++ F + N+ TM + NF N K +L F F+ H +
Sbjct: 62 KRSD-DLWMGIL-DGVSVLFRMHNMSTMRDCNFPVNSFKDCGYVLMFSKEFEDIEHLKYA 119
Query: 158 KELFIHNFGVPPQARKSKPFIDHIMSFSIVDEKIWVRTYEISHEARNNVEYEDKEEDETS 217
K++ H F +S D + F +D IWVR Y+I +
Sbjct: 120 KDVIEHIF-------RSNETKDKALCFFYLDGVIWVRCYKIGKK---------------- 156
Query: 218 LVEIGPRFVMTPILILEGSFGGPKIYE 244
L EIGPR V+ + +LE F G +Y+
Sbjct: 157 LEEIGPRLVLEVLKVLEKCFEGKALYK 183
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.320 0.138 0.405
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,068,324
Number of Sequences: 60738
Number of extensions: 740372
Number of successful extensions: 1599
Number of sequences better than 1.0e-05: 9
Number of HSP's better than 0.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 1577
Number of HSP's gapped (non-prelim): 9
length of query: 298
length of database: 30,389,216
effective HSP length: 106
effective length of query: 192
effective length of database: 23,950,988
effective search space: 4598589696
effective search space used: 4598589696
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)