ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV4609 suspect: LH T KOG3739 Signal transduction mechanisms Stress-activated MAP kinase-interacting protein, Sin1p
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV4609 1585943 1582647 -1099
(1099 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YOL078w [T] KOG3739 Stress-activated MAP kinase-interacting prot... 497 e-140
SPAPYUG7.02c [T] KOG3739 Stress-activated MAP kinase-interacting... 103 1e-21
>YOL078w [T] KOG3739 Stress-activated MAP kinase-interacting protein Sin1p
Length = 1176
Score = 497 bits (1279), Expect = e-140
Identities = 333/888 (37%), Positives = 463/888 (51%), Gaps = 86/888 (9%)
Query: 281 KEKRNNSSSSSLALDEKVKSTKRTNFLENFPLNPHNILQTSDTVSSNDSERRHNSQAKRG 340
+E N + S+ L ++++ F + N Q SD++++N S A
Sbjct: 306 RESSNGNIGSASRLKSHFPTSQKGKI---FLTDNKNDGQKSDSLNANKGIHGDGSSASGN 362
Query: 341 GKQKEYDNGKDASSYI---ESYLNQPDLKPFDLQNGITIETALNNTDSD--AINSKSDGI 395
G + S+ I ESY+N+ DL D N T+ + +N T SD S +DG
Sbjct: 363 GSVSRDGLTETESNNISDMESYINEKDL---DDLNFDTVTSNINKTVSDLGGHESTNDGT 419
Query: 396 P------RXXXXXXXXXXXXXXXXXXXASSYGKSLLXXXXXXXXXXXXXXXXXXXXXMTF 449
+ SSYGKSLL M+
Sbjct: 420 AVMNRDSKDSRSNSNEFNAQNRDRITPGSSYGKSLLGSEYSEERYSNNDSSTMESGEMSL 479
Query: 450 DS----HEIPNNSIPRS-RALTMYHSGDDSTLDKELDKATKLLHMANKAPRES----LSQ 500
DS + IP++SIP S + +YH DDSTL+ DKA ++ +++ P+E +S
Sbjct: 480 DSDMQTNTIPSHSIPMSMQKYGIYHGDDDSTLNNVFDKAVLTMN-SSRHPKERRDTVISG 538
Query: 501 SEPRESTGAVGKRGL--NLSQPELXXXXXXXXXXXXXXXXXXXXXHLLPIERHRRTSSVA 558
EP T + K + NL+ + HR S
Sbjct: 539 KEPTSLTSSNRKFSVSSNLTSTRSPLLRGHGRTSSTASSEHMKAPKVSDSVLHRARKSTL 598
Query: 559 T------QSSVDKDTIEPSTEPEFVIEKVDVGNNHPQSESTLSILFNKSK----TTQINP 608
T Q SV + S E +IEK S LS +FNK K T ++
Sbjct: 599 TLKQDHSQPSVPSSVHKSSKEGNILIEKTTDYLVSKPKASQLSNMFNKKKKRTNTNSVDV 658
Query: 609 LEYFCAVSAEKELRSNALKLDVYIQDSKTYKRKPFTISVKKTATVFEVLGYSLYCYSTEF 668
LEYF V +K ++ L++YIQ SK YKR FT V+K++T+FEV+G++L+ YSTE
Sbjct: 659 LEYFSFVCGDKVPNYESMGLEIYIQASKKYKRNSFTTKVRKSSTIFEVIGFALFLYSTEK 718
Query: 669 KPPDTAGE-LPTDEINNPNHFTLKIVDEDGEPYEDNFGVLERTQQINTIFDNEVVICKVL 727
KP + + L ++I+NPN+F+LKIVDEDGEP+EDNFG L+R I +I D+EVV+CKV
Sbjct: 719 KPDNFEEDGLTVEDISNPNNFSLKIVDEDGEPFEDNFGKLDRKSTIQSISDSEVVLCKV- 777
Query: 728 NDEQFERNEEETPLP---------SVMTDDFDQEDSNDGLSHLNQLSYYKSIIPSKNTQM 778
+D + +NE ETPLP + D D+ DG +NQLS+YK II +++
Sbjct: 778 DDAEKSQNEIETPLPFETGGGLMDASTLDANSSHDTTDGT--INQLSFYKPIIGNEDDID 835
Query: 779 SDSGSNYVNIKVFLYPNLNPQYNFTNIRIPVTSKINEVLVNYCKLKNLDPTEYVLKMEKR 838
+GS +++ V+LYPN+NP++N+T I + VTS IN++LV YCK+KN+DP EY LK+ +
Sbjct: 836 KTNGSKIIDVTVYLYPNVNPKFNYTTISVLVTSHINDILVKYCKMKNMDPNEYALKVLGK 895
Query: 839 NLILDLNDYVTSLDGNYNVELLKKKDARTKKFERMKAVTNQPVLPTIQSTELTPLTLTMA 898
N ILDLND V LDG VEL+ KKDAR E+MK +PVLPTIQS +LTPLTL
Sbjct: 896 NYILDLNDTVLRLDGINKVELISKKDARELHLEKMKPDLKKPVLPTIQSNDLTPLTL--- 952
Query: 899 NDNRLLNKTLQEEETSANSGRPTSNRT-------SSKNLFSINKQHNTS---SSTXXXXX 948
LN L+ + A + P + + S+K + KQH++S S +
Sbjct: 953 ---EPLNSYLKADAGGAVAAIPENTKVTSKAKKISTKYKLGLAKQHSSSSVASGSVSTAG 1009
Query: 949 XXXXXXXXXXXXXXXXXXXXXXXXXNNVSGNNS---------------YKDLFAGSYYKY 993
+N++ + S ++DLF G+Y+KY
Sbjct: 1010 GLANGNGFFKNKNSSKSSLHGTLQFHNINRSQSTMEHTPDTPNGVGDNFQDLFTGAYHKY 1069
Query: 994 KVWRRQQMSFINKHERMLVIDGDYVYISGPDGDFNWTHENVKTKSFHISQITLVKRSKRV 1053
KVWRRQQMSFINKHER L IDGDY+YI P+G +W H+NVKTKS HISQ+ LVK+SKRV
Sbjct: 1070 KVWRRQQMSFINKHERTLAIDGDYIYIVPPEGRIHW-HDNVKTKSLHISQVVLVKKSKRV 1128
Query: 1054 PEYFKIFVHRPDRE--RRYYFEAVSAEECVEIITRLQNLIRVYRMNQK 1099
PE+FKIFV R ++ +RYYFEAVS +EC EI+TRLQNL+ YRMN K
Sbjct: 1129 PEHFKIFVRREGQDDIKRYYFEAVSGQECTEIVTRLQNLLSAYRMNHK 1176
Score = 55.1 bits (131), Expect = 6e-07
Identities = 36/117 (30%), Positives = 62/117 (52%), Gaps = 11/117 (9%)
Query: 1 MDVEVIINKLRAKFLKQSSERDQLNRIIKPFGEVSE---------TTLKLYEDENGENML 51
MD ++N+LRA+FL+ E+DQ+ RIIKP+ V E + +LY + +G ++L
Sbjct: 1 MDTVTVLNELRAQFLRVCPEKDQMKRIIKPYIPVDEFNTEQCLDSSIRELYMNSDGVSLL 60
Query: 52 QRLDSPPIAESSVK-YGRHSKNHSI-GTESNLFSASQNNTMVSLVEEEEEGTDRVRN 106
L+SPP+++ ++ Y K + E A+QN E +EE T +++
Sbjct: 61 PELESPPVSKDFMENYASLGKMRIMRENEGQKGKANQNLIGAEKTERDEEETRNLQD 117
>SPAPYUG7.02c [T] KOG3739 Stress-activated MAP kinase-interacting protein Sin1p
Length = 665
Score = 103 bits (258), Expect = 1e-21
Identities = 84/299 (28%), Positives = 145/299 (48%), Gaps = 16/299 (5%)
Query: 583 VGNNHPQSESTLSILFNKSKTTQINPL-EYFCAVSAEKELRSNALKLDVYIQDSKTYKRK 641
V N + + ++L +K ++Q PL E F S E SNAL+L++Y S++ K
Sbjct: 248 VSNAKAPTSALRALLEHKENSSQNGPLAENFATFSGHAE--SNALRLNIYFPSSES-PSK 304
Query: 642 PFTISVKKTATVFEVLGYSLYCYSTEFKPPDTAGELPTDEINNPNHFTLKIVDEDGEPYE 701
P + ++K V E +GY L Y + P DE NPN++ L+IV++DGE E
Sbjct: 305 PLFVELRKNVLVSEAIGYILLQYVNQQLVPPIE-----DEAQNPNYWNLRIVEDDGELDE 359
Query: 702 DNFGVLERTQQINTIFDNEVVICKVLNDEQFERNEEETPLPSVMTDDFDQEDSNDGLSHL 761
D F L+R ++ + + K Q + N+ P S + ++ + H
Sbjct: 360 D-FPALDRVGPLSKFGFDAFALVKA-TPAQIKENQAAYPFKSKHPTSIPEANNKTHIRHT 417
Query: 762 NQLSYYKSIIPSKNTQMSDSGSNYVNIKVFLYPNLNPQYNFTNIRIPVTSKINEVLVNYC 821
+ S +S +++ + + + S+ V +++ P F NI I T+++ VL C
Sbjct: 418 SSTSS-QSQKQAQDVKDTLNTSHVVQVRL---PPYGDNARFCNIEISKTTRLAMVLNQVC 473
Query: 822 KLKNLDPTEYVLKMEKRNLILDLNDYVTSLDGNYNVELLKKKDARTKKFERMKAVTNQP 880
+K L+ +Y L++ + +L L+ +SLDGN +EL+KKK R KK + T+ P
Sbjct: 474 WMKQLERFKYTLRVAGSDTVLPLDKTFSSLDGNPTLELVKKK-VRDKKGSTQQLPTSSP 531
Score = 81.3 bits (199), Expect = 8e-15
Identities = 44/116 (37%), Positives = 69/116 (58%), Gaps = 2/116 (1%)
Query: 980 NSYKDLFAGSYYKYKVWRRQQMSFINKHERMLVIDGDYVYISGPDGDFNWTHENVKTKSF 1039
N+ + + +Y ++ VW+RQ +SF+ +HER+L IDG+YV+I P N E KT S
Sbjct: 549 NATDIMSSNTYQEFLVWKRQPVSFMGRHERLLAIDGEYVHIM-PSESKN-IFETPKTSSI 606
Query: 1040 HISQITLVKRSKRVPEYFKIFVHRPDRERRYYFEAVSAEECVEIITRLQNLIRVYR 1095
H I L K+SK+ P FK+ V + +RY FE +SA E I++R++ L+ +
Sbjct: 607 HAGSIILCKQSKKSPCNFKMIVSKNRETKRYDFEVLSALEAAIIVSRIRALMNTVK 662
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.311 0.129 0.353
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 55,860,825
Number of Sequences: 60738
Number of extensions: 2293604
Number of successful extensions: 6187
Number of sequences better than 1.0e-05: 2
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 6171
Number of HSP's gapped (non-prelim): 5
length of query: 1099
length of database: 30,389,216
effective HSP length: 117
effective length of query: 982
effective length of database: 23,282,870
effective search space: 22863778340
effective search space used: 22863778340
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)