ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV4844 good K KOG2593 Transcription Transcription initiation factor IIE, alpha subunit
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV4844 1657394 1658983 530
(530 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YKL028w [K] KOG2593 Transcription initiation factor IIE alpha su... 390 e-108
SPAPYUG7.01 [K] KOG2593 Transcription initiation factor IIE alph... 110 5e-24
Hs5031727 [K] KOG2593 Transcription initiation factor IIE alpha ... 108 2e-23
7294692 [K] KOG2593 Transcription initiation factor IIE alpha su... 96 2e-19
ECU07g0220 [K] KOG2593 Transcription initiation factor IIE alpha... 81 3e-15
CE16735 [K] KOG2593 Transcription initiation factor IIE alpha su... 76 1e-13
At1g03280 [K] KOG2593 Transcription initiation factor IIE alpha ... 75 2e-13
At4g20340 [K] KOG2593 Transcription initiation factor IIE alpha ... 75 3e-13
At4g20810 [K] KOG2593 Transcription initiation factor IIE alpha ... 60 1e-08
>YKL028w [K] KOG2593 Transcription initiation factor IIE alpha subunit
Length = 482
Score = 390 bits (1001), Expect = e-108
Identities = 205/407 (50%), Positives = 264/407 (64%), Gaps = 49/407 (12%)
Query: 1 MDKPIEETIKALLSFVIRQFYPNSYVLVLDAIMFHSVLSEEDLAHLLGIKRPELRSLITV 60
MD+PI++ +K LL FV+R FY S+VLVLDAI+FHSVL+E+DL LL I + EL LI
Sbjct: 1 MDRPIDDIVKNLLKFVVRGFYGGSFVLVLDAILFHSVLAEDDLKQLLSINKTELGPLIAR 60
Query: 61 LLEDRILARHSQQEFAVNSRSVKRYYYYIKYPQAIDAIKWKVHQIVAKLKDDLDKNSTPQ 120
L DR+++ H Q+E+ NS+SV+R YYY+KYP AIDAIKWKVHQ+V +LKDDLDKNS P
Sbjct: 61 LRSDRLISIHKQREYPPNSKSVERVYYYVKYPHAIDAIKWKVHQVVQRLKDDLDKNSEPN 120
Query: 121 GYICPLCSTRFTQLEAIALLNYERTQFVCSLCEEPLVEDDSGKRSKEKQGRLNRLMDQVQ 180
GY+CP+C T++TQLEA+ LLN++RT+F+CSLC+EPLVEDDSGK++KEKQ +LNRLMDQ+Q
Sbjct: 121 GYMCPICLTKYTQLEAVQLLNFDRTEFLCSLCDEPLVEDDSGKKNKEKQDKLNRLMDQIQ 180
Query: 181 PIIDYLKKIDDNMIEENTFETALARLIPPQNNSVAQYTLNPRARKSRMFVPGD------- 233
PIID LKKIDD+ IEENTFE ALARLIPPQN S A YT NP+ + S MF PGD
Sbjct: 181 PIIDSLKKIDDSRIEENTFEIALARLIPPQNQSHAAYTYNPK-KGSTMFRPGDSAPLPNL 239
Query: 234 --VTMGNDNSRKAGVKSQATLHVNITTXXXXXXXXXXXXXXXXXXXXXNALPAWHEKSTI 291
+GND+SR+AG SQATLH+NITT NA+P WH++STI
Sbjct: 240 MGTALGNDSSRRAGANSQATLHINITTASDEVAQRELQERQAEEKRKQNAVPEWHKQSTI 299
Query: 292 GESALGNLRDEYDMSSGIKIENDEETEGXXXXXXXXXXXXXXDNNNEGFXXXXXXXXXXX 351
G++ALG +++N+EE + + E
Sbjct: 300 GKTALG------------RLDNEEEFDPVVTASAMDSINPDNEPAQETSYQN-------- 339
Query: 352 XXXXXXXXXXXXXXXXPIIRSTLTDEQMKEREAERTLAEYYAQLARK 398
TLT+++M+ERE E+TL +YYA LA+K
Sbjct: 340 -------------------NRTLTEQEMEERENEKTLNDYYAALAKK 367
>SPAPYUG7.01 [K] KOG2593 Transcription initiation factor IIE alpha subunit
Length = 372
Score = 110 bits (275), Expect = 5e-24
Identities = 66/235 (28%), Positives = 112/235 (47%), Gaps = 24/235 (10%)
Query: 64 DRILARHSQQEFAVNS-RSVKRYYYYIKYPQAIDAIKWKVHQIVAKLKDDLDKNSTPQGY 122
DR+LA S+ E R Y+YI + ID+IKW++HQ+V ++D + + +GY
Sbjct: 1 DRLLAIQSRTEMKEGQQRQYHTTYFYIDFCSTIDSIKWRMHQLVKTVEDRMRNDFDSKGY 60
Query: 123 ICPLCSTRFTQLEAIALLNYERTQFVCSLCEEPLVEDDSGKRSKEKQGRLNRLMDQVQPI 182
+CP C+ +F+ L+ ++L+ E T F C++C L +D+ Q RL +LM QV I
Sbjct: 61 VCPFCNKKFSSLDVLSLVTNEGT-FACNVCGTELKDDEESAEMMSSQKRLGKLMGQVNGI 119
Query: 183 IDYLKKIDDNMIEENTFETALARLIPP--QNNSVAQYTLNPRARKSRMFVPG-DVTMGND 239
ID LK++D+ ++ +N F++AL +P +++Q L+ R+ +T+
Sbjct: 120 IDALKRVDEIVVPQNNFQSALEHAVPVSLDTQNLSQQNLSKSNSDVRLSTSSPSITVDFS 179
Query: 240 NSRKAGVKSQATLHVNITTXXXXXXXXXXXXXXXXXXXXXNALPAWHEKSTIGES 294
++ K + + N LP WH STI S
Sbjct: 180 ADKETDEKRERNCDKQV-------------------KAAQNILPEWHATSTISGS 215
>Hs5031727 [K] KOG2593 Transcription initiation factor IIE alpha subunit
Length = 439
Score = 108 bits (271), Expect = 2e-23
Identities = 55/187 (29%), Positives = 101/187 (53%)
Query: 5 IEETIKALLSFVIRQFYPNSYVLVLDAIMFHSVLSEEDLAHLLGIKRPELRSLITVLLED 64
+ +K L +VIR FY + L LD ++ +S + EED+ LL R +LRS++ L D
Sbjct: 10 VPAALKRLAKYVIRGFYGIEHALALDILIRNSCVKEEDMLELLKFDRKQLRSVLNNLKGD 69
Query: 65 RILARHSQQEFAVNSRSVKRYYYYIKYPQAIDAIKWKVHQIVAKLKDDLDKNSTPQGYIC 124
+ + + E A + ++ + YY+I Y ++ +K+K+ + +++ D ++ + C
Sbjct: 70 KFIKCRMRVETAADGKTTRHNYYFINYRTLVNVVKYKLDHMRRRIETDERDSTNRASFKC 129
Query: 125 PLCSTRFTQLEAIALLNYERTQFVCSLCEEPLVEDDSGKRSKEKQGRLNRLMDQVQPIID 184
P+CS+ FT LEA L + F C+ C + ED+S K+ + L R +Q++PI
Sbjct: 130 PVCSSTFTDLEANQLFDPMTGTFRCTFCHTEVEEDESAMPKKDARTLLARFNEQIEPIYA 189
Query: 185 YLKKIDD 191
L++ +D
Sbjct: 190 LLRETED 196
>7294692 [K] KOG2593 Transcription initiation factor IIE alpha subunit
Length = 429
Score = 95.5 bits (236), Expect = 2e-19
Identities = 47/186 (25%), Positives = 97/186 (51%)
Query: 5 IEETIKALLSFVIRQFYPNSYVLVLDAIMFHSVLSEEDLAHLLGIKRPELRSLITVLLED 64
+ ++K L V+R FY L++D ++ + + E+D+ LL ++ +LR+ IT L D
Sbjct: 24 VPSSLKQLARLVVRGFYSLEDALIIDMLVRNPCMKEDDIGELLRFEKKQLRARITTLRTD 83
Query: 65 RILARHSQQEFAVNSRSVKRYYYYIKYPQAIDAIKWKVHQIVAKLKDDLDKNSTPQGYIC 124
+ + + E + ++ K YY+I Y ++ +K+K+ + +++ + ++ + C
Sbjct: 84 KFIQIRLKMETGPDGKAQKVNYYFINYKTFVNVVKYKLDLMRKRMETEERDATSRASFKC 143
Query: 125 PLCSTRFTQLEAIALLNYERTQFVCSLCEEPLVEDDSGKRSKEKQGRLNRLMDQVQPIID 184
CS FT LEA L + +F C+ C + ED + K+ + L +Q+QP+ D
Sbjct: 144 SSCSKTFTDLEADQLFDMATLEFRCTFCGSSVEEDSAAMPKKDSRLMLAHFNEQLQPLYD 203
Query: 185 YLKKID 190
L++++
Sbjct: 204 LLREVE 209
>ECU07g0220 [K] KOG2593 Transcription initiation factor IIE alpha subunit
Length = 291
Score = 81.3 bits (199), Expect = 3e-15
Identities = 49/185 (26%), Positives = 97/185 (51%), Gaps = 5/185 (2%)
Query: 6 EETIKALLSFVIRQFYPNSYVLVLDAIMFHSVLSEEDLAHLLGIKRPELRSLITVLLEDR 65
E + L+ V+R+FY +V++ D ++ ++L + +L + + E+ LI L ED+
Sbjct: 4 EPVMNDLIKKVVRKFYEPHHVVIADILLRKTLLYDTELCERMKMLSKEVNRLIIKLREDK 63
Query: 66 ILARHSQQEFAVNSRSVKRYYYYIKYPQAIDAIKWKVHQIVAKLKDDLDKNSTPQGYICP 125
I+ ++ E ++ + R YYI Y + D IK+K+ ++ L++++ K + +GY+C
Sbjct: 64 IIKYETKVESREDNGQILRTVYYINYAEVRDVIKYKIFKMTKNLENNI-KMAQVEGYVCM 122
Query: 126 LCSTRFTQLEAIALLNYERTQFVCSLCEEPLVEDDSGKRSKEKQGRLNRLMDQVQPIIDY 185
C ++ L+A L+ E F C C+ LVE+ + + + LM ++ I+
Sbjct: 123 ECGKEYSSLDAQCLM--ENYVFKCEDCKGDLVENKKDRSADCMM--YSNLMSELDDIVLL 178
Query: 186 LKKID 190
LK+ D
Sbjct: 179 LKETD 183
>CE16735 [K] KOG2593 Transcription initiation factor IIE alpha subunit
Length = 433
Score = 75.9 bits (185), Expect = 1e-13
Identities = 39/203 (19%), Positives = 98/203 (48%)
Query: 5 IEETIKALLSFVIRQFYPNSYVLVLDAIMFHSVLSEEDLAHLLGIKRPELRSLITVLLED 64
I E + +L V++ F+ + + +++ IM + EE+L + + LR L+ L +
Sbjct: 23 IPEALNTILLMVVKNFFSSEHFIIVYHIMRAQCIREENLKARIQFDQKMLRQLLASLKAE 82
Query: 65 RILARHSQQEFAVNSRSVKRYYYYIKYPQAIDAIKWKVHQIVAKLKDDLDKNSTPQGYIC 124
+++ + + N+R+V +YYI Y ++ +++K+ + KL+ ++ Y C
Sbjct: 83 KLVKERTITQKNENNRTVSIIFYYINYRAVLNVVRYKIDHMRQKLESREQMDTNRAHYRC 142
Query: 125 PLCSTRFTQLEAIALLNYERTQFVCSLCEEPLVEDDSGKRSKEKQGRLNRLMDQVQPIID 184
C + + LE +L+ E + +C C ++ D++ S+ + + R +Q+ P+
Sbjct: 143 GACQSSYDMLEINRILDAESGRLICWRCHGDVLADETVVPSRTTRTAVARFNEQMTPLFS 202
Query: 185 YLKKIDDNMIEENTFETALARLI 207
++ ++ + + E + + +
Sbjct: 203 HICALNGIQLAPHLLEPDITKYL 225
>At1g03280 [K] KOG2593 Transcription initiation factor IIE alpha subunit
Length = 506
Score = 75.1 bits (183), Expect = 2e-13
Identities = 55/213 (25%), Positives = 102/213 (47%), Gaps = 43/213 (20%)
Query: 27 LVLDAIMFHSVLSEEDLAHLLGIKRPELRSLITVLLEDRILARHSQQEFAVNSR------ 80
+VLDA+ + EEDLA L + +LR +I + E++++ R ++E A ++
Sbjct: 52 VVLDALARRQWVREEDLAKDLQLHAKQLRKIIRLFEEEKLIMRDHRKETAKGAKMYSAAV 111
Query: 81 ----------SVKRY---YYYIKYPQA---------IDAIKWKVHQIVAKLKDDLDKNST 118
VK + Y + Y QA D +++++H++ +LKD+L+ +T
Sbjct: 112 AATTDGRAEDKVKLHTHSYCCLDYAQARFISSFLQICDVVRFRLHRMKKRLKDELEDKNT 171
Query: 119 PQGYICPLCSTRFTQLEAIALLNYERTQFVCSLC-------------EEPLVEDDSGKRS 165
Q Y CP C ++ L+A+ L++ F C C EE + DD+ +R
Sbjct: 172 VQEYGCPNCQRKYNALDALRLISMVDDSFHCENCNGELVVECNKLTSEEVVDGDDNARRR 231
Query: 166 KEK--QGRLNRLMDQVQPIIDYLKKIDDNMIEE 196
+ + + L +L Q++P++D L ++ D I E
Sbjct: 232 RRENLKNMLQKLEVQMKPLMDQLNRVKDLPIPE 264
>At4g20340 [K] KOG2593 Transcription initiation factor IIE alpha subunit
Length = 527
Score = 74.7 bits (182), Expect = 3e-13
Identities = 55/197 (27%), Positives = 90/197 (44%), Gaps = 36/197 (18%)
Query: 7 ETIKALLSFVIRQFYPN-----------------SYVLVLDAIMFHSVLSEEDLAHLLGI 49
E L+ ++R FY N S V+VLDA+ + EEDLA +
Sbjct: 15 EPFVKLVRLLVRIFYDNYTPESDNQQKSVKNVKGSAVIVLDALTRRQWVREEDLAKEVKR 74
Query: 50 KRPELRSLITVLLEDRILARHSQQEFAVNSR----------------SVKRY---YYYIK 90
ELR LI E + + R+ ++E A ++ +VK + Y +
Sbjct: 75 NAKELRKLIRHFEEQKFVMRYHRKETAKRAKMYSYAVGGTTDGRAEDNVKFHTHSYCCLD 134
Query: 91 YPQAIDAIKWKVHQIVAKLKDDLDKNSTPQGYICPLCSTRFTQLEAIALLNYERTQFVCS 150
Y Q D +++K+H++ K KD+L+ +T Q Y CP C ++ L+A+ L++ E F C
Sbjct: 135 YAQIYDIVRYKLHRLKKKFKDELEDRNTVQEYGCPNCKRKYNALDALRLISMEDDSFHCE 194
Query: 151 LCEEPLVEDDSGKRSKE 167
C LV + + S+E
Sbjct: 195 NCNGELVMECNKLISEE 211
>At4g20810 [K] KOG2593 Transcription initiation factor IIE alpha subunit
Length = 416
Score = 59.7 bits (143), Expect = 1e-08
Identities = 55/214 (25%), Positives = 94/214 (43%), Gaps = 20/214 (9%)
Query: 26 VLVLDAIMFHSVLSEEDLAHLLGIKRPELRSLITVLLEDRILARHSQQEFA---VNSRSV 82
VLVLDA+ + EEDLA L + +LR+++ E + + R ++E + N R
Sbjct: 33 VLVLDALTRRQWVREEDLAKELKLNTKQLRTILRYFEEQQFIMRVHRKEKSSATTNGRGE 92
Query: 83 KRY------YYYIKYPQAIDAIKWKVHQIVAKLKDDLDKNSTPQGYICPLCSTR-FTQLE 135
+ Y + Y Q D I++K+H++ + KD L+ Q Y CP C + F E
Sbjct: 93 DKVKVHMYSYCCLDYSQIYDVIRYKLHRMKKEFKDVLEDKDNVQEYGCPNCKRKIFFHCE 152
Query: 136 AIALLNYERTQFVCSLCEEPLVEDDSG---KRSKEKQGRLNRLMDQVQPIIDYLKKIDDN 192
N E L E +V D S R + L + +++P++D++ +I D
Sbjct: 153 N---CNGELVMECNKLTSEEVVVDGSDNPRSRRDHLKDLLQNMEVRLKPLMDHINRIKDL 209
Query: 193 MIEE----NTFETALARLIPPQNNSVAQYTLNPR 222
+ +ET +A+ + TL P+
Sbjct: 210 PVPSFESFPAWETRVAKAARENGDLNPDDTLRPQ 243
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.312 0.130 0.358
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 23,157,095
Number of Sequences: 60738
Number of extensions: 822130
Number of successful extensions: 1914
Number of sequences better than 1.0e-05: 9
Number of HSP's better than 0.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 1898
Number of HSP's gapped (non-prelim): 11
length of query: 530
length of database: 30,389,216
effective HSP length: 111
effective length of query: 419
effective length of database: 23,647,298
effective search space: 9908217862
effective search space used: 9908217862
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)