ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV4848 good O KOG2018 Posttranslational modification, protein turnover, chaperones Predicted dinucleotide-utilizing enzyme involved in molybdopterin and thiamine biosynthesis
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV4848 1659258 1660595 446
(446 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YKL027w [O] KOG2018 Predicted dinucleotide-utilizing enzyme invo... 499 e-141
YHR003c [O] KOG2018 Predicted dinucleotide-utilizing enzyme invo... 405 e-113
SPAC1A6.10 [O] KOG2018 Predicted dinucleotide-utilizing enzyme i... 325 1e-88
At5g37530 [O] KOG2018 Predicted dinucleotide-utilizing enzyme in... 216 7e-56
YHR111w [H] KOG2017 Molybdopterin synthase sulfurylase 52 2e-06
CE28320 [H] KOG2017 Molybdopterin synthase sulfurylase 50 9e-06
>YKL027w [O] KOG2018 Predicted dinucleotide-utilizing enzyme involved in
molybdopterin and thiamine biosynthesis
Length = 447
Score = 499 bits (1285), Expect = e-141
Identities = 243/443 (54%), Positives = 308/443 (68%)
Query: 4 DNWRVIASTAAITFAGVKFIDLAIKSWNKRTAASYSGSDNEKDVKLAKRLRETREYDNLL 63
D W++I +TA T A D A SW + + K + + +YD
Sbjct: 5 DTWKLITATALFTVAVTTITDYAWTSWQAQKQVIAQQKNKNKGGQTKSDTDKYHQYDEQF 64
Query: 64 YREQLARNYAFLGEEGMEKLKEQYFIXXXXXXXXXXXXTMLVRSGACRIKIIDFDQVSLS 123
R+ L N FLGE+ +EKL QY + LVRSG +I+++DFDQVSLS
Sbjct: 65 IRQSLKNNVEFLGEDTIEKLSNQYVVVVGAGGVGSWVVNSLVRSGCRKIRVVDFDQVSLS 124
Query: 124 SLNRHSCADLYDVGLSKVNVLRDHLQKIAPWCNIEVCNELWNKENADRLIFGDGTPTFVI 183
SLNRHSCA L DVG KV LR H+++IAPWC I+ NELW +N +RL G+GTP F++
Sbjct: 125 SLNRHSCAILNDVGTPKVECLRRHMREIAPWCEIDPINELWTLQNGERLTLGNGTPDFIV 184
Query: 184 DCIDNLDTKVDLLEYTYKKNIPVIASMGAATKSDPTRINVGDLTMTEEDPLARTVRRRLK 243
DCIDN+DTKVDLLE+ Y I VI+SMGA+ KSDPT++NVGDL TEEDPLAR VRR+LK
Sbjct: 185 DCIDNIDTKVDLLEFAYNHGIKVISSMGASAKSDPTKLNVGDLATTEEDPLARVVRRKLK 244
Query: 244 QRGITKGIPAVFSXXXXXXXXXXXXXXXXXXFQKGNVDQLSALKDFRVRILPVLGTMPGV 303
+RGI GIP VFS +++G VD+LSALKDFRVRILPVLGTMP +
Sbjct: 245 KRGILSGIPVVFSAEKPDPKKAKLLPLPDEEYERGKVDELSALKDFRVRILPVLGTMPSL 304
Query: 304 FGLTIATWVLCKISGYPMEPIEGKNRIKLYDGIYQSLAGQMTRIGQKDQRIPIALSDIGY 363
FGLTI TW+L IS P+EP+EGKNRIK+YDGIYQSLAGQM+R+G QRIP+AL D+ Y
Sbjct: 305 FGLTITTWILSNISDKPLEPVEGKNRIKVYDGIYQSLAGQMSRVGIPSQRIPLALKDVSY 364
Query: 364 IVEEVWRGKSPISNYSTRLTLSKWDPTKPVSIQNVILLTKDEQREHEARILNGTETFSDV 423
+VEEV++GKSPIS STRLTL+KWDP+KP+S+QNV++LTK+EQ+ HE R+L G E+ DV
Sbjct: 365 LVEEVFKGKSPISGISTRLTLTKWDPSKPISLQNVVVLTKNEQKVHEDRVLKGKESLQDV 424
Query: 424 YSEEALAMINKRFQEELYYSQFR 446
Y + L ++++RF+EE YYSQFR
Sbjct: 425 YDAKVLKLVSQRFREEAYYSQFR 447
>YHR003c [O] KOG2018 Predicted dinucleotide-utilizing enzyme involved in
molybdopterin and thiamine biosynthesis
Length = 429
Score = 405 bits (1040), Expect = e-113
Identities = 211/445 (47%), Positives = 288/445 (64%), Gaps = 18/445 (4%)
Query: 1 MGNDNWRVIASTAAITFAGVKFIDLAIKSWNKRTAASYSGSDNEKDVKLAKRLRETREYD 60
M N+ W++IA+TA I+ + K + AA+ + K + R+YD
Sbjct: 1 MANNTWKLIATTALISVFSTQLAKSVWKEYKLSCAANKN-----------KTVSRPRQYD 49
Query: 61 NLLYREQLARNYAFLGEEGMEKLKEQYFIXXXXXXXXXXXXTMLVRSGACRIKIIDFDQV 120
+ L+REQLARNYAFLGEEGM K+KEQY + TML+RSG +I IID + +
Sbjct: 50 DHLFREQLARNYAFLGEEGMRKIKEQYIVIVGAGEVGSWVCTMLIRSGCQKIMIIDPENI 109
Query: 121 SLSSLNRHSCADLYDVGLSKVNVLRDHLQKIAPWCNIEVCNELWNKENADRLIFGDG-TP 179
S+ SLN H CA L D+G KV L++HL KIAPW I+ + W KEN+ LIF DG +P
Sbjct: 110 SIDSLNTHCCAVLSDIGKPKVQCLKEHLSKIAPWSEIKARAKAWTKENSHDLIFADGESP 169
Query: 180 TFVIDCIDNLDTKVDLLEYTYKKNIPVIASMGAATKSDPTRINVGDLTMTEEDPLARTVR 239
TF++DC+DNL++KVDLLEY + I VI+SMG ATKSDPTR+++ D++MTE DP++R VR
Sbjct: 170 TFIVDCLDNLESKVDLLEYAHHNKIDVISSMGVATKSDPTRVSINDISMTEFDPISRCVR 229
Query: 240 RRLKQRGITKGIPAVFSXXXXXXXXXXXXXXXXXXFQKGNVDQLSALKDFRVRILPVLGT 299
R+L++RGI GI VFS + ++A++D +R LP LGT
Sbjct: 230 RKLRKRGIATGISVVFSNEMLDPRRDDILSPIDCEHR-----AINAVRDEALRHLPELGT 284
Query: 300 MPGVFGLTIATWVLCKISGYPMEPIEGKNRIKLYDGIYQSLAGQMTRIGQKDQRIP-IAL 358
MPG+FGL+IATW+L K+SGYPM+ E KNR+K YD I ++ QM R+ + +R + L
Sbjct: 285 MPGIFGLSIATWILTKVSGYPMKENEVKNRLKFYDSILETFQKQMARLNENKERSSLLGL 344
Query: 359 SDIGYIVEEVWRGKSPISNYSTRLTLSKWDPTKPVSIQNVILLTKDEQREHEARILNGTE 418
++GYIVEE++RGKSPIS YST+L L+KW+ K +S+ NV+L+TK+EQ HE RIL E
Sbjct: 345 EEVGYIVEEMFRGKSPISGYSTKLALTKWEANKEISLTNVVLMTKEEQEIHEKRILLDGE 404
Query: 419 TFSDVYSEEALAMINKRFQEELYYS 443
+ VYSEE L I + F+EE YYS
Sbjct: 405 KLTAVYSEEVLDFIERLFKEEEYYS 429
>SPAC1A6.10 [O] KOG2018 Predicted dinucleotide-utilizing enzyme involved in
molybdopterin and thiamine biosynthesis
Length = 485
Score = 325 bits (832), Expect = 1e-88
Identities = 174/389 (44%), Positives = 240/389 (60%), Gaps = 9/389 (2%)
Query: 59 YDNLLYREQLARNYAFLGEEGMEKLKEQYFIXXXXXXXXXXXXTMLVRSGACRIKIIDFD 118
YD L REQLARNYAF GE+GME+L+ + I ML RSG +I+I+DFD
Sbjct: 100 YDENLIREQLARNYAFFGEDGMERLRNSFVIVVGCGGVGSWVINMLARSGVQKIRIVDFD 159
Query: 119 QVSLSSLNRHSCADLYDVGLSKVNVLRDHLQKIAPWCNIEVCNELWNKENADRLIFGDGT 178
QVSLSSLNRHS A L DVG K ++ ++K APW ++ N L+N ++AD L+ G
Sbjct: 160 QVSLSSLNRHSIATLQDVGTPKTLAIKKAIKKFAPWIEVDARNALFNPDSADDLL--SGN 217
Query: 179 PTFVIDCIDNLDTKVDLLEYTYKKNIPVIASMGAATKSDPTRINVGDLTMTEEDPLARTV 238
P FVID IDN+ TKVDLL Y Y +PVIAS G+A KSDPTR+N+ D++ T EDPL+R
Sbjct: 218 PDFVIDAIDNIQTKVDLLSYCYNHKLPVIASTGSACKSDPTRVNIADISATSEDPLSRAT 277
Query: 239 RRRLKQRGITKGIPAVFSXXXXXXXXXXXXXXXXXXFQKGNVDQLSALKDFRVRILPVLG 298
RRRL+ GI +GIP VFS F+KG+VD+LSAL +FR RILPV+G
Sbjct: 278 RRRLRLLGIMEGIPVVFSTEKPDPRKASLLPLSEEEFEKGDVDELSALPEFRARILPVIG 337
Query: 299 TMPGVFGLTIATWVLCKISGYPMEPIEGKNRIKLYDGIYQSLAGQMTRIGQKDQRIPIAL 358
MPG+FGLTIAT+VL I+ YPM+PI R +LY+ + L + + G +
Sbjct: 338 PMPGIFGLTIATYVLTSIAKYPMDPISTMTRPRLYEEAVKRLHAEARKAGVNLDK-TFNA 396
Query: 359 SDIGYIVEEVWRGKSPISNY-STRLTLSKWDPTKPVSIQNVILLTKDEQREHEARILNGT 417
S++ YI+EEV+ G+S + + S ++T+ +W+P P N++ +T++E R HE +L
Sbjct: 397 SEMSYIIEEVYVGRSALPPHESQKVTVVRWNPQLPFDHTNLVAMTRNEARYHEDNVLAKN 456
Query: 418 ETFSDVYSEEALAMINK-----RFQEELY 441
S VY ++ + +++ R E LY
Sbjct: 457 VDPSTVYGKDVIEVVHSFLRRLRMWEMLY 485
>At5g37530 [O] KOG2018 Predicted dinucleotide-utilizing enzyme involved in
molybdopterin and thiamine biosynthesis
Length = 414
Score = 216 bits (549), Expect = 7e-56
Identities = 129/383 (33%), Positives = 199/383 (51%), Gaps = 20/383 (5%)
Query: 66 EQLARNYAFLGEEGMEKLKEQYFIXXXXXXXXXXXXTMLVRSGACRIKIIDFDQVSLSSL 125
E L RN F G E K+ Y + +ML+RSG ++ ++DFDQVSLSSL
Sbjct: 31 EHLTRNIQFFGLESQHKVTGSYVVVIGLGGVGSHAASMLLRSGVGKLLLVDFDQVSLSSL 90
Query: 126 NRHSCADLYDVGLSKVNVLRDHLQKIAPWCNIEVCNELWNKENADRLIFGDGTPTFVIDC 185
NRH+ A DVG+ K L+ H I P C+IE L++ + + ++ G P FV+DC
Sbjct: 91 NRHAVATRADVGIPKAMCLKKHFSSIFPECHIEAKVMLYDSSSEEEIL--SGKPDFVLDC 148
Query: 186 IDNLDTKVDLLEYTYKKNIPVIASMGAATKSDPTRINVGDLTMTEEDPLARTVRRRLKQR 245
IDN+DTKV LL K+ + V+++ GA ++DPTRI V D+ + DPL+R+VR RL+++
Sbjct: 149 IDNIDTKVGLLAACVKRGLKVLSATGAGARADPTRIRVADIRESTIDPLSRSVRHRLRRK 208
Query: 246 -GITKGIPAVFSXXXXXXXXXXXXXXXXXXFQKGNVDQLSALKDFRVRILPVLGTMPGVF 304
GI GIP VFS + N + FRVRI+PVLGT+P +F
Sbjct: 209 HGIEGGIPVVFSLEKPKAKLLPFEGTNG---EDENPLDYQVVPGFRVRIIPVLGTIPAIF 265
Query: 305 GLTIATWVLCKISG--YPMEPIEGKNRIKLYDGIYQSLAGQMTRIGQKDQRIPIALSDIG 362
G +A++V+ +++G MEPI + + Y ++Q L + + + + ++
Sbjct: 266 GQIMASYVITQLAGVQVQMEPIVNLD-LDHYRLLHQRLIEHEETVYGTSAEVEVDVEEVM 324
Query: 363 YIVEEVWRGKSPISNYS-----------TRLTLSKWDPTKPVSIQNVILLTKDEQREHEA 411
YIV+E+W G+S + L L +WD KP ++ N++LL E EHE
Sbjct: 325 YIVKELWHGRSARDETAKDVGRGMWRAMNELMLVRWDAKKPATVSNLVLLKFKEADEHET 384
Query: 412 RILNGTETFSDVYSEEALAMINK 434
+ L + + E ++ K
Sbjct: 385 KTLEEVKESETEFFERVSCVLKK 407
>YHR111w [H] KOG2017 Molybdopterin synthase sulfurylase
Length = 440
Score = 52.0 bits (123), Expect = 2e-06
Identities = 45/183 (24%), Positives = 75/183 (40%), Gaps = 7/183 (3%)
Query: 48 KLAKRLRETREYDNLLYREQLARNYAFL----GEEGMEKLKEQYFIXXXXXXXXXXXXTM 103
+LAKR +R+Y L Q + G G KLK +
Sbjct: 26 QLAKREDSSRDYPLSLEEYQRYGRQMIVEETGGVAGQVKLKNTKVLVVGAGGLGCPALPY 85
Query: 104 LVRSGACRIKIIDFDQVSLSSLNRHSCADLYDVGLSKVNVLRDHLQKIAPWCNIEVCNEL 163
L +G +I I+D D V S+L+R D VG+ K R ++ K+ P N+
Sbjct: 86 LAGAGVGQIGIVDNDVVETSNLHRQVLHDSSRVGMLKCESARQYITKLNPHINVVTYPVR 145
Query: 164 WNKENADRLIFGDGTPTFVIDCIDNLDTKVDLLEYTYKKNIPVIASMGAATKSDPTRINV 223
N NA + G +++DC D+ T+ + + I V+++ G T+ T +N
Sbjct: 146 LNSSNAFDIFKG---YNYILDCTDSPLTRYLVSDVAVNLGITVVSASGLGTEGQLTILNF 202
Query: 224 GDL 226
++
Sbjct: 203 NNI 205
>CE28320 [H] KOG2017 Molybdopterin synthase sulfurylase
Length = 402
Score = 49.7 bits (117), Expect = 9e-06
Identities = 39/142 (27%), Positives = 63/142 (43%), Gaps = 5/142 (3%)
Query: 76 GEEGMEKLKEQYFIXXXXXXXXXXXXTMLVRSGACRIKIIDFDQVSLSSLNRHSCADLYD 135
G G + LK + T L +G I I+D+D +SL +L+R
Sbjct: 28 GVSGQKNLKNLNVLIVGAGGLGCPVATYLGAAGIGTIGIVDYDHISLDNLHRQVAYKEDQ 87
Query: 136 VGLSKVNVLRDHLQKIAPWCNIEVCNELWNKENADRLIFGDGTPTFVIDCIDNLDTKVDL 195
VG SK L D+++ N++V N + NA +L V DC DN+ T+ +
Sbjct: 88 VGKSKAQALADNIKLQNSDLNVQVHNTSLDSSNAMQLF---KNYEIVCDCTDNVATRYLI 144
Query: 196 LEYTYKKNIPVIASMGAATKSD 217
+ NIP+++ G+A + D
Sbjct: 145 NDVCVLLNIPLVS--GSALRWD 164
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.319 0.136 0.400
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 24,238,815
Number of Sequences: 60738
Number of extensions: 982010
Number of successful extensions: 2420
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 2400
Number of HSP's gapped (non-prelim): 6
length of query: 446
length of database: 30,389,216
effective HSP length: 110
effective length of query: 336
effective length of database: 23,708,036
effective search space: 7965900096
effective search space used: 7965900096
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)