ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV5062 good I KOG4232 Lipid transport and metabolism Delta 6-fatty acid desaturase/delta-8 sphingolipid desaturase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV5062 1732372 1730654 -573
(573 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
At3g61580 [I] KOG4232 Delta 6-fatty acid desaturase/delta-8 sphi... 212 1e-54
At2g46210 [I] KOG4232 Delta 6-fatty acid desaturase/delta-8 sphi... 210 5e-54
CE25113 [I] KOG4232 Delta 6-fatty acid desaturase/delta-8 sphing... 136 8e-32
CE25153 [I] KOG4232 Delta 6-fatty acid desaturase/delta-8 sphing... 135 2e-31
Hs11181775 [I] KOG4232 Delta 6-fatty acid desaturase/delta-8 sph... 101 3e-21
Hs13375616 [I] KOG4232 Delta 6-fatty acid desaturase/delta-8 sph... 95 3e-19
Hs4758334 [I] KOG4232 Delta 6-fatty acid desaturase/delta-8 sphi... 91 4e-18
>At3g61580 [I] KOG4232 Delta 6-fatty acid desaturase/delta-8 sphingolipid
desaturase
Length = 449
Score = 212 bits (540), Expect = 1e-54
Identities = 126/369 (34%), Positives = 196/369 (52%), Gaps = 20/369 (5%)
Query: 194 IASEYRKLHGEITAAGLYQCN-----YVRYLKEFLRIGTLFGISFYLLSLKWFA--ISAI 246
++ +YR++ E GL++ Y + +G L+G+ L FA I+A
Sbjct: 90 VSRDYRRMAAEFRKLGLFENKGHVTLYTLAFVAAMFLGVLYGV---LACTSVFAHQIAAA 146
Query: 247 CLGFAWQQLVFIAHDAGHISITHNYQVDNIIGMTVASWIGGLSLGWWKRNHNVHHLVTND 306
LG W Q +I HD+GH I N + + + + G+S+ WWK HN HHL N
Sbjct: 147 LLGLLWIQSAYIGHDSGHYVIMSNKSYNRFAQLLSGNCLTGISIAWWKWTHNAHHLACNS 206
Query: 307 PVHDPDIQHLPFFAVSTRLFHNVYSTYYDKFLWFDKFAQKVVPIQHYLYYPILCFGRFNL 366
+DPD+QH+P FAVST+ F ++ S +YD+ L FD A+ +V QH+ YYP++CFGR NL
Sbjct: 207 LDYDPDLQHIPVFAVSTKFFSSLTSRFYDRKLTFDPVARFLVSYQHFTYYPVMCFGRINL 266
Query: 367 YRLSWMHVLLGQGPRRGKAAWFRYYELAGLSFFNYWFFYLIIYKQMPTNAERFKYVMISH 426
+ +++ +L + +A F AG+ F W ++ ++ +P ERF +V S
Sbjct: 267 FIQTFL-LLFSKREVPDRALNF-----AGILVF--WTWFPLLVSCLPNWPERFFFVFTSF 318
Query: 427 IATMIVHVQITLSHFAMSTSDLGVTES-FPMRQLRTSMDVDCPRWLDFFHGGLQFQVIHH 485
T + H+Q TL+HFA T S + +Q ++D+ C ++D+F GGLQFQ+ HH
Sbjct: 319 TVTALQHIQFTLNHFAADVYVGPPTGSDWFEKQAAGTIDISCRSYMDWFFGGLQFQLEHH 378
Query: 486 LFPRLPRHNLRDAQSLVIKFCDKVGIKYSIYGFAAGNDVVISHLQQIAQQAHTMLE-CAK 544
LFPRLPR +LR +V + C K + Y + N + I+ L+ A QA + K
Sbjct: 379 LFPRLPRCHLRKVSPVVQELCKKHNLPYRSMSWFEANVLTINTLKTAAYQARDVANPVVK 438
Query: 545 TMKKEATDT 553
+ EA +T
Sbjct: 439 NLVWEALNT 447
>At2g46210 [I] KOG4232 Delta 6-fatty acid desaturase/delta-8 sphingolipid
desaturase
Length = 449
Score = 210 bits (534), Expect = 5e-54
Identities = 117/349 (33%), Positives = 185/349 (52%), Gaps = 15/349 (4%)
Query: 194 IASEYRKLHGEITAAGLYQCNYVRYLKEFLRIGTLFGISFY----LLSLKWFAISAICLG 249
++ +YR+L E + GL+ L +G + Y S+ ISA+ LG
Sbjct: 90 VSRDYRRLAAEFSKRGLFDKKGHVTLYTLTCVGVMLAAVLYGVLACTSIWAHLISAVLLG 149
Query: 250 FAWQQLVFIAHDAGHISITHNYQVDNIIGMTVASWIGGLSLGWWKRNHNVHHLVTNDPVH 309
W Q ++ HD+GH ++T + +I + + + G+S+ WWK HN HH+ N H
Sbjct: 150 LLWIQSAYVGHDSGHYTVTSTKPCNKLIQLLSGNCLTGISIAWWKWTHNAHHIACNSLDH 209
Query: 310 DPDIQHLPFFAVSTRLFHNVYSTYYDKFLWFDKFAQKVVPIQHYLYYPILCFGRFNLYRL 369
DPD+QH+P FAVST+ F+++ S +Y + L FD A+ ++ QH+ +YP++C GR NL+
Sbjct: 210 DPDLQHIPIFAVSTKFFNSMTSRFYGRKLTFDPLARFLISYQHWTFYPVMCVGRINLFIQ 269
Query: 370 SWMHVLLGQGPRRGKAAWFRYYELAGLSFFNYWFFYLIIYKQMPTNAERFKYVMISHIAT 429
+++ + + R +AG+ F WF L+ + +P ERF +V +S T
Sbjct: 270 TFLLLF------SKRHVPDRALNIAGILVFWTWFPLLVSF--LPNWQERFIFVFVSFAVT 321
Query: 430 MIVHVQITLSHFA--MSTSDLGVTESFPMRQLRTSMDVDCPRWLDFFHGGLQFQVIHHLF 487
I HVQ L+HFA + T + F +Q ++D+ C ++D+F GGLQFQ+ HHLF
Sbjct: 322 AIQHVQFCLNHFAADVYTGPPNGNDWFE-KQTAGTLDISCRSFMDWFFGGLQFQLEHHLF 380
Query: 488 PRLPRHNLRDAQSLVIKFCDKVGIKYSIYGFAAGNDVVISHLQQIAQQA 536
PRLPR +LR +V + C K + Y + N I L+ A QA
Sbjct: 381 PRLPRCHLRTVSPVVKELCKKHNLPYRSLSWWEANVWTIRTLKNAAIQA 429
>CE25113 [I] KOG4232 Delta 6-fatty acid desaturase/delta-8 sphingolipid
desaturase
Length = 447
Score = 136 bits (343), Expect = 8e-32
Identities = 108/381 (28%), Positives = 179/381 (46%), Gaps = 44/381 (11%)
Query: 185 SLDVKNQEWIASEYRKLHGEITAAGLYQCNYVRYLKEFLRIGTLFGI--SFYLLSLKWFA 242
++ K I + L + A GL + + Y+++ L T+F I +FYL ++
Sbjct: 96 NISEKRSAQINKSFTDLRMRVRAEGLMDGSPLFYIRKILE--TIFTILFAFYLQYHTYYL 153
Query: 243 ISAICLGFAWQQLVFIAHDAGHISITHNYQVDNIIGMTVASWIGGLSLGWWKRNHNVHHL 302
SAI +G AWQQL ++ H+ H + N +++ V +++ G S G WK HNVHH
Sbjct: 154 PSAILMGVAWQQLGWLIHEFAHHQLFKNRYYNDLASYFVGNFLQGFSSGGWKEQHNVHHA 213
Query: 303 VTNDPVHDPDIQHLPFFAVSTRLFHNVYSTYYDKFLWFDKFAQKVVPIQHYLYYPILCFG 362
TN D D+ +PF+A +N YS D + + QH + +L F
Sbjct: 214 ATNVVGRDGDLDLVPFYATVAEHLNN-YSQ--------DSWVMTLFRWQHVHWTFMLPF- 263
Query: 363 RFNLYRLSWM---HVLLGQGPRRGKAAWFRY------YELAGLSFFNYWFFYLIIYKQMP 413
RLSW+ + + Q P ++ Y YE GLS +W + L +P
Sbjct: 264 ----LRLSWLLQSIIFVSQMPTH----YYDYYRNTAIYEQVGLSL--HWAWSLGQLYFLP 313
Query: 414 TNAERFKYVMISHI--ATMIVHVQITLSHFAMSTSDL--GVTESFPMRQLRTSMDVDCPR 469
+ R + ++SH+ ++ HV +T +H+++ L + ++ Q+ T+ ++ R
Sbjct: 314 DWSTRIMFFLVSHLVGGFLLSHV-VTFNHYSVEKFALSSNIMSNYACLQIMTTRNMRPGR 372
Query: 470 WLDFFHGGLQFQVIHHLFPRLPRHNLRDAQSLVIKFCDKVGIKYSIYGFAAGNDVVISHL 529
++D+ GGL +Q+ HHLFP +PRHNL LV +F G+ Y + + G + I
Sbjct: 373 FIDWLWGGLNYQIEHHLFPTMPRHNLNTVMPLVKEFAAANGLPYMVDDYFTGFWLEIEQF 432
Query: 530 QQIAQQAHTMLECAKTMKKEA 550
+ IA A AK KK A
Sbjct: 433 RNIANVA------AKLTKKIA 447
>CE25153 [I] KOG4232 Delta 6-fatty acid desaturase/delta-8 sphingolipid
desaturase
Length = 443
Score = 135 bits (339), Expect = 2e-31
Identities = 93/353 (26%), Positives = 162/353 (45%), Gaps = 14/353 (3%)
Query: 186 LDVKNQEWIASEYRKLHGEITAAGLYQCNYVRYLKEFLRIGTLFGISFYLLSLKWFAISA 245
+ V ++ + + KL ++ GL + N +L + + ++ +FYL L W+ SA
Sbjct: 94 VSVAQEKKMVESFEKLRQKLHDDGLMKANETYFLFKAISTLSIMAFAFYLQYLGWYITSA 153
Query: 246 ICLGFAWQQLVFIAHDAGHISITHNYQVDNIIGMTVASWIGGLSLGWWKRNHNVHHLVTN 305
L AWQQ ++ H+ H T N +++ I + +++ G S WWK HN HH TN
Sbjct: 154 CLLALAWQQFGWLTHEFCHQQPTKNRPLNDTISLFFGNFLQGFSRDWWKDKHNTHHAATN 213
Query: 306 DPVHDPDIQHLPFFAVSTRLFHNVYSTYYDKFLWFDKFAQKVVPIQHYLYYPILCFGRFN 365
HD DI P FA ++ F+K K+VP QH + +L RF+
Sbjct: 214 VIDHDGDIDLAPLFAFIPGDLCKYKAS-------FEKAILKIVPYQHLYFTAMLPMLRFS 266
Query: 366 LYRLSWMHVLLGQGPRRGKAAWFRYYELAGLSFFNYWFFYLIIYKQMPTNAERFKYVMIS 425
S V ++E A + W FY + +PT R Y +IS
Sbjct: 267 WTGQSVQWVFKENQMEYKVYQRNAFWEQATIVGHWAWVFYQLFL--LPTWPLRVAYFIIS 324
Query: 426 HI--ATMIVHVQITLSHFAMS--TSDLGVTESFPMRQLRTSMDVDCPRWLDFFHGGLQFQ 481
+ +I HV +T +H ++ ++ + +F Q+ T+ ++ ++D+ GGL +Q
Sbjct: 325 QMGGGLLIAHV-VTFNHNSVDKYPANSRILNNFAALQILTTRNMTPSPFIDWLWGGLNYQ 383
Query: 482 VIHHLFPRLPRHNLRDAQSLVIKFCDKVGIKYSIYGFAAGNDVVISHLQQIAQ 534
+ HHLFP +PR NL V ++C + + Y + + G + + L+ +A+
Sbjct: 384 IEHHLFPTMPRCNLNACMKYVKEWCKENNLPYLVDDYFDGYAMNLQQLKNMAE 436
>Hs11181775 [I] KOG4232 Delta 6-fatty acid desaturase/delta-8 sphingolipid
desaturase
Length = 444
Score = 101 bits (252), Expect = 3e-21
Identities = 91/366 (24%), Positives = 151/366 (40%), Gaps = 43/366 (11%)
Query: 184 PSLDVKNQEWIASEYRKLHGEITAAGLYQCNYVRYLKEFLRIGTLFGISFYLL-----SL 238
PS + + + E+R+L + GL + N+V +L L I L G ++ L S
Sbjct: 98 PSFEPTKNKELTDEFRELRATVERMGLMKANHVFFLLYLLHILLLDGAAWLTLWVFGTSF 157
Query: 239 KWFAISAICLGFAWQQLVFIAHDAGHISITHNYQVDNIIGMTVASWIGGLSLGWWKRNHN 298
F + A+ L Q ++ HD GH+S+ + ++++ V + G WW H
Sbjct: 158 LPFLLCAVLLSAVQAQAGWLQHDFGHLSVFSTSKWNHLLHHFVIGHLKGAPASWWNHMHF 217
Query: 299 VHHLVTNDPVHDPDIQHLPFFAVSTRLF----------HNVYSTYYDKFLWFDKFAQKVV 348
HH N DPDI PFF ++ + Y+ + F A +
Sbjct: 218 QHHAKPNCFRKDPDINMHPFFFALGKILSVELGKQKKKYMPYNHQHKYFFLIGPPALLPL 277
Query: 349 PIQHYLYYPILCFGRFNLYRLSWMHVLLGQGPRRGKAAWFRYYELAGLSFFNYWFFYLII 408
Q Y++Y ++ R L+WM + + Y L GL F FF I
Sbjct: 278 YFQWYIFYFVI--QRKKWVDLAWMITFYVR-------FFLTYVPLLGLKAFLGLFF---I 325
Query: 409 YKQMPTNAERFKYVMISHIATMIVHVQITLSHFAMSTSDLGVTESFPMRQLRTSMDVDCP 468
+ + +N +V ++ + + +H+ + +ST QL+ + +V
Sbjct: 326 VRFLESNW----FVWVTQMNHIPMHIDHDRNMDWVST------------QLQATCNVHKS 369
Query: 469 RWLDFFHGGLQFQVIHHLFPRLPRHNLRDAQSLVIKFCDKVGIKYSIYGFAAGNDVVISH 528
+ D+F G L FQ+ HHLFP +PRHN LV C K GI+Y + +I
Sbjct: 370 AFNDWFSGHLNFQIEHHLFPTMPRHNYHKVAPLVQSLCAKHGIEYQSKPLLSAFADIIHS 429
Query: 529 LQQIAQ 534
L++ Q
Sbjct: 430 LKESGQ 435
>Hs13375616 [I] KOG4232 Delta 6-fatty acid desaturase/delta-8 sphingolipid
desaturase
Length = 445
Score = 95.1 bits (235), Expect = 3e-19
Identities = 84/347 (24%), Positives = 138/347 (39%), Gaps = 39/347 (11%)
Query: 184 PSLDVKNQEWIASEYRKLHGEITAAGLYQCN---YVRYLKEFLRIGTLFGISFYLLSLKW 240
PS D + ++R LH L+ + + L L + L + YLL W
Sbjct: 101 PSQDGPLNAQLVEDFRALHQAAEDMKLFDASPTFFAFLLGHILAMEVLAWLLIYLLGPGW 160
Query: 241 F--AISAICLGFAWQQLVFIAHDAGHISITHNYQVDNIIGMTVASWIGGLSLGWWKRNHN 298
A++A L + Q + HD GH SI +++ V + G S WW H
Sbjct: 161 VPSALAAFILAISQAQSWCLQHDLGHASIFKKSWWNHVAQKFVMGQLKGFSAHWWNFRHF 220
Query: 299 VHHLVTNDPVHDPDIQHLPFFAVSTRLFHNVYSTYYDKFLWFDKFAQKVVPI-QHYLYY- 356
HH N DPD+ P F + + + + K ++ +P Q +LY+
Sbjct: 221 QHHAKPNIFHKDPDVTVAPVFLLG------------ESSVEYGKKKRRYLPYNQQHLYFF 268
Query: 357 ----PILCFGRFNLYRLSWMHVLLGQGPRRGKAAWFRYYELAGLSFFNYWFFYLIIYKQM 412
P+L F + L++M V + A+++ + L+ L F+ L
Sbjct: 269 LIGPPLLTLVNFEVENLAYMLVCMQWADLLWAASFYARFFLSYLPFYGVPGVLLFFVAVR 328
Query: 413 PTNAERFKYV-MISHIATMIVHVQITLSHFAMSTSDLGVTESFPMRQLRTSMDVDCPRWL 471
+ F ++ ++HI I H + + QL + +V+ +
Sbjct: 329 VLESHWFVWITQMNHIPKEIGHEK---------------HRDWVSSQLAATCNVEPSLFT 373
Query: 472 DFFHGGLQFQVIHHLFPRLPRHNLRDAQSLVIKFCDKVGIKYSIYGF 518
++F G L FQ+ HHLFPR+PRHN LV C K G+ Y + F
Sbjct: 374 NWFSGHLNFQIEHHLFPRMPRHNYSRVAPLVKSLCAKHGLSYEVKPF 420
>Hs4758334 [I] KOG4232 Delta 6-fatty acid desaturase/delta-8 sphingolipid
desaturase
Length = 444
Score = 91.3 bits (225), Expect = 4e-18
Identities = 87/340 (25%), Positives = 137/340 (39%), Gaps = 34/340 (10%)
Query: 184 PSLDVKNQEWIASEYRKLHGEITAAGLYQCNYVRYLKEFLRIGTLFGISFYLLSL---KW 240
PS D I ++R L L++ N+V +L I L I+++ + W
Sbjct: 99 PSQDHGKNSKITEDFRALRKTAEDMNLFKTNHVFFLLLLAHIIALESIAWFTVFYFGNGW 158
Query: 241 FA--ISAICLGFAWQQLVFIAHDAGHISITHNYQVDNIIGMTVASWIGGLSLGWWKRNHN 298
I+A L + Q ++ HD GH+S+ + ++++ V + G S WW H
Sbjct: 159 IPTLITAFVLATSQAQAGWLQHDYGHLSVYRKPKWNHLVHKFVIGHLKGASANWWNHRHF 218
Query: 299 VHHLVTNDPVHDPDIQHLPFFAVSTRLFHNVYSTYYDKFLWFDKFAQKVVPIQH-----Y 353
HH N DPD+ L F + + + + K K +P H +
Sbjct: 219 QHHAKPNIFHKDPDVNMLHVFVLGEW-----------QPIEYGKKKLKYLPYNHQHEYFF 267
Query: 354 LYYPILCFGRFNLYRLSWMHVLLGQGPRRGKAAWFRYYELAGLSFFNYWFFYLIIYKQMP 413
L P L + Y++ ++ AW Y + F Y FY I+ +
Sbjct: 268 LIGPPLLIPMYFQYQIIMTMIVHKNWV---DLAWAVSYYIR--FFITYIPFYGILGALLF 322
Query: 414 TNAERFKYVMISHIATMIVHVQITLSHFAMSTSDLGVTESFPMRQLRTSMDVDCPRWLDF 473
N RF + SH + ++H M + F QL + +V+ + D+
Sbjct: 323 LNFIRF---LESHWFVWVTQ----MNHIVMEIDQEAYRDWFSS-QLTATCNVEQSFFNDW 374
Query: 474 FHGGLQFQVIHHLFPRLPRHNLRDAQSLVIKFCDKVGIKY 513
F G L FQ+ HHLFP +PRHNL LV C K GI+Y
Sbjct: 375 FSGHLNFQIEHHLFPTMPRHNLHKIAPLVKSLCAKHGIEY 414
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.325 0.140 0.443
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 36,113,469
Number of Sequences: 60738
Number of extensions: 1620800
Number of successful extensions: 4535
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 4512
Number of HSP's gapped (non-prelim): 13
length of query: 573
length of database: 30,389,216
effective HSP length: 112
effective length of query: 461
effective length of database: 23,586,560
effective search space: 10873404160
effective search space used: 10873404160
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)