ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV5082 good U KOG3337 Intracellular trafficking, secretion, and vesicular transport Protein similar to predicted member of the intramitochondrial sorting protein family
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV5082 1738957 1739478 174
(174 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YLR193c [U] KOG3337 Protein similar to predicted member of the i... 209 2e-54
SPAP8A3.10 [U] KOG3337 Protein similar to predicted member of th... 129 2e-30
Hs7019509 [U] KOG3337 Protein similar to predicted member of the... 97 1e-20
Hs20473176 [U] KOG3337 Protein similar to predicted member of th... 93 2e-19
Hs22044072 [U] KOG3337 Protein similar to predicted member of th... 92 3e-19
YLR168c [U] KOG3336 Predicted member of the intramitochondrial s... 84 1e-16
SPBC36.10 [U] KOG3336 Predicted member of the intramitochondrial... 83 2e-16
Hs7705610 [U] KOG3336 Predicted member of the intramitochondrial... 81 8e-16
CE02936 [U] KOG3337 Protein similar to predicted member of the i... 76 3e-14
7297061 [U] KOG3336 Predicted member of the intramitochondrial s... 75 6e-14
7303894 [U] KOG3337 Protein similar to predicted member of the i... 73 2e-13
YDR185c [U] KOG3336 Predicted member of the intramitochondrial s... 70 1e-12
CE15853 [U] KOG3336 Predicted member of the intramitochondrial s... 70 2e-12
At5g13070 [U] KOG3336 Predicted member of the intramitochondrial... 54 8e-08
Hs20543572 [U] KOG3336 Predicted member of the intramitochondria... 53 2e-07
>YLR193c [U] KOG3337 Protein similar to predicted member of the
intramitochondrial sorting protein family
Length = 175
Score = 209 bits (531), Expect = 2e-54
Identities = 102/172 (59%), Positives = 130/172 (75%), Gaps = 2/172 (1%)
Query: 1 MVLWHNNSHTFGNSFDAVTIAFFNRYPNPYASHVLSIDTLSRRVDDDGRLISIRLIKKMG 60
MVL H ++H F F +V+ AFFNRYPNPY+ HVLSIDT+SR VD +G L + RL+KK G
Sbjct: 1 MVLLHKSTHIFPTDFASVSRAFFNRYPNPYSPHVLSIDTISRNVDQEGNLRTTRLLKKSG 60
Query: 61 KLPNWVKPFLGKISESWIIEHSVVDTKKGEMLTYTRNLDHTKIIKVEEYTRFEKDG--NS 118
KLP WVKPFL I+E+WIIE SVV+ M TYTRNLDHT I+KVEEYT ++ D +S
Sbjct: 61 KLPTWVKPFLRGITETWIIEVSVVNPANSTMKTYTRNLDHTGIMKVEEYTTYQFDSATSS 120
Query: 119 TQVKSMVKFSSGFGIGIRNRIESWSHSKFDENIQKSRKGMTFVLQRLDLSLN 170
T S VKFSSGF +GI++++E WS +KFDEN++KSR GM FV+Q+L+ + N
Sbjct: 121 TIADSRVKFSSGFNMGIKSKVEDWSRTKFDENVKKSRMGMAFVIQKLEEARN 172
>SPAP8A3.10 [U] KOG3337 Protein similar to predicted member of the
intramitochondrial sorting protein family
Length = 171
Score = 129 bits (324), Expect = 2e-30
Identities = 64/154 (41%), Positives = 101/154 (65%), Gaps = 4/154 (2%)
Query: 14 SFDAVTIAFFNRYPNPYASHVLSIDTLSRRVDDDGRLISIRLIKKMGKLPNWVKPFLGKI 73
S++ V+ A+ RYPNPY+ HV+S D L R VDD+GRL + RL+ K G+LP W L +
Sbjct: 14 SWNTVSSAWLTRYPNPYSLHVVSADVLERYVDDEGRLYTERLLVKQGRLPRWASDLL-NV 72
Query: 74 SESWIIEHSVVDTKKGEMLTYTRNLDHTKIIKVEEYTRF---EKDGNSTQVKSMVKFSSG 130
++S+I+E SV+D K E+ + T NLDH KI++V EY+RF ++ + T V ++ KF S
Sbjct: 73 NKSYILERSVIDPSKQELKSETFNLDHVKILRVIEYSRFIQSSENCSKTIVDTIAKFVSP 132
Query: 131 FGIGIRNRIESWSHSKFDENIQKSRKGMTFVLQR 164
G+ R++ +S +F E + SR+G+ +V+Q+
Sbjct: 133 LRFGLGRRVQKYSLKRFQEQLSSSRRGLLYVIQQ 166
>Hs7019509 [U] KOG3337 Protein similar to predicted member of the
intramitochondrial sorting protein family
Length = 219
Score = 96.7 bits (239), Expect = 1e-20
Identities = 53/170 (31%), Positives = 94/170 (55%), Gaps = 6/170 (3%)
Query: 1 MVLWHNNSHTFGNSFDAVTIAFFNRYPNPYASHVLSIDTLSRRVDDDGRLISIRLIKKMG 60
MV + +S+D V AF+ RYPNPY+ HVL+ D + R V D +L+S RL+ K
Sbjct: 1 MVKYFLGQSVLRSSWDQVFAAFWQRYPNPYSKHVLTEDIVHREVTPDQKLLSRRLLTKTN 60
Query: 61 KLPNWV-KPFLGKISES-WIIEHSVVDTKKGEMLTYTRNLDHTKIIKVEE---YTRFEKD 115
++P W + F ++ S +++E S+VD + M T+T N++H +++ VEE Y +
Sbjct: 61 RMPRWAERLFPANVAHSVYVLEDSIVDPQNQTMTTFTWNINHARLMVVEERCVYCVNSDN 120
Query: 116 GNSTQVKSMVKFSSGFGIGIRNRIESWSHSKFDENIQKSRKGMTFVLQRL 165
T+++ SS G+ ++ + ++F N+ K+ KG ++L +L
Sbjct: 121 SGWTEIRREAWVSSSL-FGVSRAVQEFGLARFKSNVTKTMKGFEYILAKL 169
>Hs20473176 [U] KOG3337 Protein similar to predicted member of the
intramitochondrial sorting protein family
Length = 250
Score = 92.8 bits (229), Expect = 2e-19
Identities = 49/170 (28%), Positives = 95/170 (55%), Gaps = 6/170 (3%)
Query: 1 MVLWHNNSHTFGNSFDAVTIAFFNRYPNPYASHVLSIDTLSRRVDDDGRLISIRLIKKMG 60
MV + +S+D V AF+++YPNPY+ HVL+ DT+ R V D +L+S +L+ K
Sbjct: 1 MVKYFLGQGVLQSSWDQVFTAFWHQYPNPYSKHVLTEDTVHREVTADQKLLSQQLLTKTN 60
Query: 61 KLPNWVKPFL--GKISESWIIEHSVVDTKKGEMLTYTRNLDHTKIIKVEEYTRFEKDGNS 118
++ +W K + +I+E S+VD + M T+T N++H +++ VE+ + + N+
Sbjct: 61 RMSHWAKQLFPANVLHSVYILEDSIVDPQNQTMTTFTWNINHARLMVVEKQCVYCMNSNN 120
Query: 119 ---TQVKSMVKFSSGFGIGIRNRIESWSHSKFDENIQKSRKGMTFVLQRL 165
T+++ SS G+ ++ + ++F N+ K+ KG ++L ++
Sbjct: 121 SGWTEIRREAWVSSSL-FGVSRSVQEFGLAQFKSNVTKTMKGFEYILAKM 169
>Hs22044072 [U] KOG3337 Protein similar to predicted member of the
intramitochondrial sorting protein family
Length = 219
Score = 92.4 bits (228), Expect = 3e-19
Identities = 52/158 (32%), Positives = 88/158 (54%), Gaps = 6/158 (3%)
Query: 13 NSFDAVTIAFFNRYPNPYASHVLSIDTLSRRVDDDGRLISIRLIKKMGKLPNWV-KPFLG 71
+S+D V AF+ RYPNPY+ HVL+ D + R V D +L+S RL+ K + P W + F
Sbjct: 13 SSWDQVFAAFWQRYPNPYSKHVLTEDVVHREVTPDQKLLSGRLLTKTNRTPCWAERLFPA 72
Query: 72 KISES-WIIEHSVVDTKKGEMLTYTRNLDHTKIIKVEE---YTRFEKDGNSTQVKSMVKF 127
+ S +I+E S+VD + M T+T N++H +++ VEE Y + T+++
Sbjct: 73 NVDHSVYILEDSIVDPQNQTMTTFTWNINHARLMVVEERCVYCVNSDNSGRTEIRREAWV 132
Query: 128 SSGFGIGIRNRIESWSHSKFDENIQKSRKGMTFVLQRL 165
SS G+ ++ + + F N+ K+ KG ++L +L
Sbjct: 133 SSSL-FGVSRAVQEFGLAWFKSNVTKTMKGFEYILAKL 169
>YLR168c [U] KOG3336 Predicted member of the intramitochondrial sorting
protein family
Length = 230
Score = 84.0 bits (206), Expect = 1e-16
Identities = 52/163 (31%), Positives = 85/163 (51%), Gaps = 6/163 (3%)
Query: 7 NSHTFGNSFDAVTIAFFNRYPNPYASHVLSIDTLSRRVDDDGR-LISIRLIKKMGKLPNW 65
NS+ F +D VT A + +YPN ++HV+++D L R + D G+ L++ RLI +P W
Sbjct: 6 NSYDFNYPWDQVTAANWKKYPNEISTHVIAVDVLRRELKDQGKVLVTERLITVKQGVPKW 65
Query: 66 VKPFLGKISESWIIEHSVVDTKKGEMLTYTRNLDHTKIIKVEEYTRF----EKDGNSTQV 121
+ LG + S + E SVVD K + + NL ++KV E + + N T
Sbjct: 66 IMMMLGGTNMSHVREVSVVDLNKKSLTMRSCNLTMCNLLKVYETVTYSPHPDDSANKTLF 125
Query: 122 KSMVKFSSGFGI-GIRNRIESWSHSKFDENIQKSRKGMTFVLQ 163
+ + ++ I + N++E WS +F EN +K + G VLQ
Sbjct: 126 QQEAQITAYGSIRKLCNKMEDWSVQRFCENAKKGKMGFDAVLQ 168
>SPBC36.10 [U] KOG3336 Predicted member of the intramitochondrial sorting
protein family
Length = 184
Score = 83.2 bits (204), Expect = 2e-16
Identities = 51/175 (29%), Positives = 88/175 (50%), Gaps = 19/175 (10%)
Query: 9 HTFGNSFDAVTIAFFNRYPNPYASHVLSIDTLSRRVDDDGRLISIRLIKKMGKLPNWVKP 68
H F F+ V+ A + +YPN +A+HV+++DTL R+V D+G L + RLI LP W+
Sbjct: 8 HLFQYPFEQVSAAHWQKYPNEHATHVIAVDTLDRKVLDNGVLYTERLITCHQALPRWILK 67
Query: 69 FLGKISESWIIEHSVVDTKKGEMLTYTRNLDHTKIIKVE-----------EYTRFEKDGN 117
+ + +I E S VD K + T NL + ++V+ E T F+++
Sbjct: 68 LIDGAQDCYIRETSYVDLKARTLTLLTSNLTFSDRLRVDETVTYSPHPELEATVFQQEAR 127
Query: 118 STQVKSMVKFSSGFGIGIRNRIESWSHSKFDENIQKSRKGMTFVLQRLDLSLNKT 172
+ M + S N IE WS F + + ++G VL+++++S+ +T
Sbjct: 128 IEALACMKRLS--------NLIEQWSVDGFGKKASRGKEGFESVLEKINMSVFQT 174
>Hs7705610 [U] KOG3336 Predicted member of the intramitochondrial sorting
protein family
Length = 211
Score = 80.9 bits (198), Expect = 8e-16
Identities = 50/178 (28%), Positives = 90/178 (50%), Gaps = 6/178 (3%)
Query: 1 MVLWHNNSHTFGNSFDAVTIAFFNRYPNPYASHVLSIDTLSRRVDDDGRLISIRLIKKMG 60
M +W + H F + ++ VT A +YPNP V+ +D L R +D G+L S RL+
Sbjct: 1 MKIW-TSEHVFDHPWETVTTAAMQKYPNPMNPSVVGVDVLDRHIDPSGKLHSHRLLSTEW 59
Query: 61 KLPNWVKPFLGKI-SESWIIEHSVVDTKKGEMLTYTRNLDHTKIIKVEE---YTRFEKDG 116
LP+ VK +G +++++ EHSVVD + M + N+ T ++ V+E Y +D
Sbjct: 60 GLPSIVKSLIGAARTKTYVQEHSVVDPVEKTMELKSTNISFTNMVSVDERLIYKPHPQDP 119
Query: 117 NSTQVKSMVKFSSGFGIGIRNRIESWSHSKFDENIQKSRKGMTFVLQRLDLSLNKTSS 174
T V + + G+ + + +E S N K R+ M +V+ +L+ + + ++
Sbjct: 120 EKT-VLTQEAIITVKGVSLSSYLEGLMASTISSNASKGREAMEWVIHKLNAEIEELTA 176
>CE02936 [U] KOG3337 Protein similar to predicted member of the
intramitochondrial sorting protein family
Length = 210
Score = 75.9 bits (185), Expect = 3e-14
Identities = 41/125 (32%), Positives = 72/125 (56%), Gaps = 7/125 (5%)
Query: 1 MVLWHNNSHTFGNSFDAVTIAFFNRYPNPYASHVLSIDTLSRRVDDDGRLISIRLIKKMG 60
M LW + + +F SFD V AF++RYPN +A H++S D L R++ D+ +++ +LI K G
Sbjct: 1 MKLWDSPNTSFPYSFDEVASAFWDRYPNSHAKHIISEDVLERQITDN-TIVTKKLIVKQG 59
Query: 61 -----KLPNWVKPFLGKISESWIIEHSVVDTKKGEMLTYTRNLDHTKIIKVEEYTRFEKD 115
++P W+ + I +IE SV D +++TYTRN+ H + ++ E ++
Sbjct: 60 SSILKRVPRWISR-MTDIQVVPVIEESVYDKVSKKLVTYTRNVSHISLFQLHERCIYKSS 118
Query: 116 GNSTQ 120
++ Q
Sbjct: 119 EDNQQ 123
>7297061 [U] KOG3336 Predicted member of the intramitochondrial sorting
protein family
Length = 215
Score = 74.7 bits (182), Expect = 6e-14
Identities = 47/165 (28%), Positives = 80/165 (48%), Gaps = 6/165 (3%)
Query: 1 MVLWHNNSHTFGNSFDAVTIAFFNRYPNPYASHVLSIDTLSRRVDDDGRLISIRLIKKMG 60
M +W + H F + ++ VT A + +YPNP ++ D + RRV D G L + RL++
Sbjct: 1 MKIW-TSEHIFNHPWETVTQAAWRKYPNPMTPSIIGTDVVERRVVD-GVLHTHRLVQSKW 58
Query: 61 KLPNWVKPFLGKISESWIIEHSVVDTKKGEMLTYTRNLDHTKIIKVEEYTRFE---KDGN 117
P W +G + E S VD ++ +M+ T NL + I V+E +E D +
Sbjct: 59 YFPKWTHALIGTAKTCFASERSTVDPERKQMVLKTNNLTFCRNISVDEVLYYEPHPSDAS 118
Query: 118 STQVKSMVKFSSGFGIGIRNRIESWSHSKFDENIQKSRKGMTFVL 162
T +K + FG+ + + +E S N K R+G+ +V+
Sbjct: 119 KTLLKQEATVTV-FGVPLSHYMEDLLTSTISTNAGKGRQGLEWVI 162
>7303894 [U] KOG3337 Protein similar to predicted member of the
intramitochondrial sorting protein family
Length = 171
Score = 73.2 bits (178), Expect = 2e-13
Identities = 39/87 (44%), Positives = 54/87 (61%), Gaps = 2/87 (2%)
Query: 18 VTIAFFNRYPNPYASHVLSIDTLSRRVDDDGRLISIRLIKKMGKLPNWVKPFLGKISESW 77
V +A++NRYPNP ++HVL+ DT+ R V DG+L S RL+ K +P W F +
Sbjct: 3 VVVAYWNRYPNPSSTHVLTEDTIQREV-RDGKLFSRRLLSKTNPVPKWGARFYNNVPVK- 60
Query: 78 IIEHSVVDTKKGEMLTYTRNLDHTKII 104
I+E SV+D K T+TRNL TKI+
Sbjct: 61 IVEDSVLDPVKKTFTTFTRNLGMTKIM 87
>YDR185c [U] KOG3336 Predicted member of the intramitochondrial sorting
protein family
Length = 179
Score = 70.1 bits (170), Expect = 1e-12
Identities = 44/161 (27%), Positives = 79/161 (48%), Gaps = 6/161 (3%)
Query: 8 SHTFGNSFDAVTIAFFNRYPNPYASHVLSIDTLSRRVDDDGR-LISIRLIKKMGKLPNWV 66
S+ F ++ VT A + +YPN ++HV+++D L R + + G L++ RLI P+W+
Sbjct: 7 SYEFDYPWEKVTTANWMKYPNKISTHVIAVDVLRRELKEHGDVLLTERLITIRQNTPHWM 66
Query: 67 KPFLGKISESWIIEHSVVDTKKGEMLTYTRNLDHTKIIKVEEYTRF----EKDGNSTQVK 122
+G + +++ E S VD + + + N+ I+K E R+ + N T K
Sbjct: 67 SILVGNTNLAYVREVSTVDRRDRSLTMRSCNMTFPHILKCYETVRYVPHPKNPSNVTLFK 126
Query: 123 SMVKFSSGFGI-GIRNRIESWSHSKFDENIQKSRKGMTFVL 162
KF SG ++E+W +F +N K + G +L
Sbjct: 127 QDAKFLSGVPTKTFSEKVENWGVKRFSDNAVKGKVGFDSIL 167
>CE15853 [U] KOG3336 Predicted member of the intramitochondrial sorting
protein family
Length = 209
Score = 69.7 bits (169), Expect = 2e-12
Identities = 44/168 (26%), Positives = 84/168 (49%), Gaps = 6/168 (3%)
Query: 1 MVLWHNNSHTFGNSFDAVTIAFFNRYPNPYASHVLSIDTLSRRVDDDGRLISIRLIKKMG 60
M +W ++ H F + ++ V A F +YPNP + ID + + ++ G++++ R+I+
Sbjct: 1 MRIW-SSEHIFDHEWETVAQAAFRKYPNPLNRSITGIDVVKQTLEA-GKILTERIIQSHF 58
Query: 61 KLPNWVKPFLGKISESWIIEHSVVDTKKGEMLTYTRNLDHTKIIKVEE---YTRFEKDGN 117
+P+W G + E++V+D + E TRNL+ + ++V+E YT +D N
Sbjct: 59 SIPSWATKLTGFSGTQYSHEYTVIDPTRKEFSLTTRNLNGSSFLRVDEKLTYTPAHEDPN 118
Query: 118 STQVKSMVKFSSGFGIGIRNRIESWSHSKFDENIQKSRKGMTFVLQRL 165
T +K V + + E S + +N K R+G+ +V+ L
Sbjct: 119 KTILKQDVIVTITLP-AFADYCEKTFLSIYSQNANKGRQGVEWVIDHL 165
>At5g13070 [U] KOG3336 Predicted member of the intramitochondrial sorting
protein family
Length = 183
Score = 54.3 bits (129), Expect = 8e-08
Identities = 41/178 (23%), Positives = 85/178 (47%), Gaps = 13/178 (7%)
Query: 1 MVLWHNNSHTFGNSFDAVTIAFFNRYPNP----YASHVLSIDTLSRRVDDD-GRLISIRL 55
MV + H + + ++ V+ A + ++ +P SH+L +DTL+R++D + G+L + R
Sbjct: 1 MVKAYRQEHVYKHPWERVSAASWRKFADPENKRILSHILEVDTLNRKLDTETGKLHTTRA 60
Query: 56 IKKMGKLPNWVKPFLGKISESWIIEHSVVDTKKGEMLTYTRNLDHTKIIKVEEYTRFEKD 115
+ P ++ +G+ +E +VVD K M T+N+ K I+VEE R++
Sbjct: 61 LTIHAPGPWFLHRIIGQ-DICHCVESTVVDGKSRSMQLTTKNISLKKFIEVEERIRYDPH 119
Query: 116 GNSTQVKSMVKFSSGFGI-------GIRNRIESWSHSKFDENIQKSRKGMTFVLQRLD 166
++ ++ + I + ++E KF +N K R+ M + + ++
Sbjct: 120 PDNPSAWTVCSQETSIRIKPLSALASMAEKVEQKCAEKFMQNSAKGREVMERICKYME 177
>Hs20543572 [U] KOG3336 Predicted member of the intramitochondrial sorting
protein family
Length = 143
Score = 52.8 bits (125), Expect = 2e-07
Identities = 29/83 (34%), Positives = 45/83 (53%), Gaps = 1/83 (1%)
Query: 27 PNPYASHVLSIDTLSRRVDDDGRLISIRLIKKMGKLPNWVKPFLGKI-SESWIIEHSVVD 85
P PY V+ +D L R VD G+L S RL+ LP+ VK +G ++++ EHSVVD
Sbjct: 13 PKPYEPTVVGVDVLDRHVDPSGKLHSHRLLSTEWGLPSIVKSLIGAARMKTYVQEHSVVD 72
Query: 86 TKKGEMLTYTRNLDHTKIIKVEE 108
+ M + N+ T ++ +E
Sbjct: 73 PVEKTMKLKSTNISFTDMVSADE 95
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.319 0.134 0.395
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,511,865
Number of Sequences: 60738
Number of extensions: 413078
Number of successful extensions: 1042
Number of sequences better than 1.0e-05: 15
Number of HSP's better than 0.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 1015
Number of HSP's gapped (non-prelim): 15
length of query: 174
length of database: 30,389,216
effective HSP length: 99
effective length of query: 75
effective length of database: 24,376,154
effective search space: 1828211550
effective search space used: 1828211550
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)