ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV5148 good J KOG2324 Translation, ribosomal structure and biogenesis Prolyl-tRNA synthetase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV5148 1763061 1764707 549
(549 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YER087w [J] KOG2324 Prolyl-tRNA synthetase 573 e-163
SPBC24C6.03 [J] KOG2324 Prolyl-tRNA synthetase 172 2e-42
7292265 [J] KOG2324 Prolyl-tRNA synthetase 148 2e-35
Hs14729163 [J] KOG2324 Prolyl-tRNA synthetase 147 5e-35
CE16514 [J] KOG2324 Prolyl-tRNA synthetase 118 3e-26
YHR020w [J] KOG4163 Prolyl-tRNA synthetase 52 2e-06
>YER087w [J] KOG2324 Prolyl-tRNA synthetase
Length = 576
Score = 573 bits (1477), Expect = e-163
Identities = 293/564 (51%), Positives = 390/564 (68%), Gaps = 21/564 (3%)
Query: 7 LFTPSKITNDILQLPT-HELLQQLGYVKQASSGVLHWLPLGLKTLRNIEGIIRRRMD-ES 64
+F P ++N+ L+ T ELLQ +G+V+++ G+ WLPLGL++L + IR RMD +
Sbjct: 13 IFHPKSLSNNTLKSETTQELLQTVGFVRRSQVGLFQWLPLGLRSLNKVSNAIRNRMDSDG 72
Query: 65 GAHEVSLSSLSQRSLWETTGRWSNTELFKLKDAKKKDYCLVPTCEEEITWLMNSYLTSYK 124
GA EVSLS++S ++LW+ T RW+N+ELFKLKD+K K YCL TCEE+IT LM +Y+ SYK
Sbjct: 73 GAIEVSLSAISSKALWQATDRWNNSELFKLKDSKGKQYCLTATCEEDITDLMKNYIASYK 132
Query: 125 DLPIIAYQITRKYRDEXXXXXXXXXXXEFLMKDAYSFHLNSEDAVSTFERVNATYDKIFT 184
D+PI YQ+TRKYRDE EFLMKDAYSF N EDA ++F++++ TY+KIF
Sbjct: 133 DMPITIYQMTRKYRDEIRPRGGILRGREFLMKDAYSFASNEEDAFASFQKLDDTYNKIFK 192
Query: 185 DIGVPFVSAVADSGDIGGDLSKEYHFIHESGEDTLFRCNSCEAVSNIEKCGSYPVESKEY 244
D+ +PFVSA ADSGDIGG+ SKE+H IHESGEDTL C C +S ++ SYP + +Y
Sbjct: 193 DLKIPFVSAWADSGDIGGEFSKEFHLIHESGEDTLMSCKHCGDISTLDMSQSYPEKDGQY 252
Query: 245 LGDVSVKYALNEENDTLVCLYYPASRHLNWNLALEAVDNDLEPQTRYMTNEEVLAKF-TE 303
GDV KYAL +++ TL+C YYP R LNWNLAL A+D D++ R N+ VL + +
Sbjct: 253 SGDVDCKYALTKDHSTLICFYYPKDRQLNWNLALNAMDKDIDLTLRNKPNDHVLQVYEKD 312
Query: 304 NYDPMFNGIIRVMDVRLNSRSNFPDFPLKSYLKNNFAQLNDYSIVDVEEGEICAQCEEGE 363
N D MF+ I+RVMD RLNS+SNFPDFPLK YLKNNF Q++D SIVD +E EIC +CEEG
Sbjct: 313 NEDIMFSKILRVMDCRLNSKSNFPDFPLKKYLKNNFGQISDVSIVDAQENEICGKCEEGR 372
Query: 364 LESAKSIEVGHTFYLGKKYSKALNATFRQQDN-SDVVMEMGCYGIGVSRLIGAIAQVTRD 422
LE KSIEVGH F LG KYSK LN F ++N ++ + MGCYGIGVSRL+GAIA++ RD
Sbjct: 373 LEPLKSIEVGHIFLLGNKYSKPLNVKFVDKENKNETFVHMGCYGIGVSRLVGAIAELGRD 432
Query: 423 PKGLRWPSSVSPYKVSVCA---SDKSVDQLNKVKALLDYKP---------YTDFDAKTSI 470
G RWP+ ++PYKVS+C + ++ +L VK+ L P F+ K I
Sbjct: 433 SNGFRWPAIMAPYKVSICTGPNNPENSQRLQDVKSELLNDPTMQNLQNDILDQFNEKLGI 492
Query: 471 GAKIQQSHALGIPIAIVVGPKSWPNVEIEVRG-NFRSKN-WETSYEKLHSQLGWEYNQ-E 527
GA+I+ SHA+GIP+ ++VG KSWPNVEIEVRG + K+ W +EK S+L W+ + E
Sbjct: 493 GARIKLSHAMGIPLCVIVGSKSWPNVEIEVRGIRWGEKDLWRKQFEKRCSELQWKCTKNE 552
Query: 528 SG--KHFVLHENANDVVNILLKDL 549
G KH V ++ +V+ +LLKD+
Sbjct: 553 HGIEKHTVPIQHLAEVIGVLLKDM 576
>SPBC24C6.03 [J] KOG2324 Prolyl-tRNA synthetase
Length = 425
Score = 172 bits (435), Expect = 2e-42
Identities = 84/212 (39%), Positives = 126/212 (58%), Gaps = 2/212 (0%)
Query: 23 HELLQQLGYVKQASSGVLHWLPLGLKTLRNIEGIIRRRMDESGAHEVSLSSLSQRSLWET 82
++LL +G+++ + G+ +LPLGL+ I ++ M GA +SL+ LS + +WE
Sbjct: 28 NQLLIDMGFIQPSMPGIFQYLPLGLRVQNKICDLLHISMRSLGASAISLAHLSSKEIWEK 87
Query: 83 TGRWSNT--ELFKLKDAKKKDYCLVPTCEEEITWLMNSYLTSYKDLPIIAYQITRKYRDE 140
+GRW T ELF+L D ++ CL PT EE++T M + + S K LPI YQI RK+RDE
Sbjct: 88 SGRWQKTGSELFRLHDRNDREMCLAPTHEEDVTRTMATIIDSQKQLPIRVYQIGRKFRDE 147
Query: 141 XXXXXXXXXXXEFLMKDAYSFHLNSEDAVSTFERVNATYDKIFTDIGVPFVSAVADSGDI 200
EF+MKD Y+F ++ A+ T+E V Y F ++G+PFV A +G+I
Sbjct: 148 LRPRGGLLRGREFMMKDLYTFDIDKASAMKTYEDVLQAYHTFFKEVGLPFVMVKAATGNI 207
Query: 201 GGDLSKEYHFIHESGEDTLFRCNSCEAVSNIE 232
GG+LS E+H+ H GED ++ C SC +N E
Sbjct: 208 GGNLSHEFHYRHPVGEDVIYTCPSCHYSTNSE 239
Score = 105 bits (262), Expect = 2e-22
Identities = 65/173 (37%), Positives = 91/173 (52%), Gaps = 12/173 (6%)
Query: 356 CAQCEEGELESAKSIEVGHTFYLGKKYSKALNATFRQQDNSDVVMEMGCYGIGVSRLIGA 415
C C + +L S +IEVGH FYLGK YS NAT + N V+ MGCYGIGVSRLI A
Sbjct: 252 CPNCND-QLTSTTAIEVGHAFYLGKIYSSKFNATVEVK-NKQEVLHMGCYGIGVSRLIAA 309
Query: 416 IAQVTRDPKGLRWPSSVSPYKVSVCASDKSVDQLNKVKALLDYKP-YTDFD-------AK 467
+A VT+D KGL WPSS++P+KV V + S + + + + D FD
Sbjct: 310 VAHVTKDAKGLVWPSSIAPWKVLVVPT--SDNHIQSAETVYDATANVVGFDNVLLEDRQN 367
Query: 468 TSIGAKIQQSHALGIPIAIVVGPKSWPNVEIEVRGNFRSKNWETSYEKLHSQL 520
+ G K++ + +G P IVVG + E+ + ++ + LH L
Sbjct: 368 RAFGYKMRDAELIGYPFVIVVGSRFQEEGICEIIVRSSGERYKLDKDSLHQVL 420
>7292265 [J] KOG2324 Prolyl-tRNA synthetase
Length = 458
Score = 148 bits (374), Expect = 2e-35
Identities = 76/214 (35%), Positives = 121/214 (56%), Gaps = 3/214 (1%)
Query: 22 THELLQQLGYVKQASSGVLHWLPLGLKTLRNIEGIIRRRMDESGAHEVSLSSLSQRSLWE 81
+ +LL +LG VK S+G +P+ +++ +++ M ++G +++L L+ LW+
Sbjct: 29 SQKLLTELGLVKSGSNGTYQIMPMAQRSVDKCIDLVQSNMQQAGGQKITLPILTPTGLWK 88
Query: 82 TTGRWSN--TELFKLKDAKKKDYCLVPTCEEEIT-WLMNSYLTSYKDLPIIAYQITRKYR 138
TGR +E + ++D K + + PT EE +T L + SY+ LP+ +QI K+R
Sbjct: 89 KTGRLDGDISEFYMVRDRSGKQFLMSPTHEEAVTAMLATTSPISYRQLPLRLFQIGPKFR 148
Query: 139 DEXXXXXXXXXXXEFLMKDAYSFHLNSEDAVSTFERVNATYDKIFTDIGVPFVSAVADSG 198
DE EFLMKD YSF ++ E A+ T+ VNA YD++F + VPFV A +G
Sbjct: 149 DELKTRFGLMRAKEFLMKDMYSFDVSEETAMETYTLVNAAYDRLFKQLEVPFVKVNAATG 208
Query: 199 DIGGDLSKEYHFIHESGEDTLFRCNSCEAVSNIE 232
+GG +S EYH++ GED L +C+SC N E
Sbjct: 209 IMGGSVSHEYHYVSPVGEDNLLQCSSCGFAGNSE 242
Score = 90.5 bits (223), Expect = 6e-18
Identities = 51/149 (34%), Positives = 74/149 (49%), Gaps = 12/149 (8%)
Query: 356 CAQCEEGELESAKSIEVGHTFYLGKKYSKALNATFRQQDNSDVVMEMGCYGIGVSRLIGA 415
C C +L+ + +EV HTF LG KYSK L ATF + MGCYGIG++R+I A
Sbjct: 250 CPSCNSSDLKEVRGVEVAHTFLLGDKYSKPLGATFLNTTGKPQSLVMGCYGIGITRVIAA 309
Query: 416 IAQVTRDPKGLRWPSSVSPYKVSVCASDKSVDQ-----------LNKVKALLDYKPYTDF 464
+V LRWP ++PY V + + + L V + ++
Sbjct: 310 ALEVLSSDHELRWPKLLAPYDVCLIGPKQGSKEQPEAEVIENELLLNVGEICGHQELLHD 369
Query: 465 DAK-TSIGAKIQQSHALGIPIAIVVGPKS 492
D K +IG ++ ++ LG P+ IVVG KS
Sbjct: 370 DRKELTIGKRLLEAKRLGHPLTIVVGAKS 398
>Hs14729163 [J] KOG2324 Prolyl-tRNA synthetase
Length = 402
Score = 147 bits (370), Expect = 5e-35
Identities = 82/208 (39%), Positives = 112/208 (53%), Gaps = 3/208 (1%)
Query: 28 QLGYVKQASSGVLHWLPLGLKTLRNIEGIIRRRMDESGAHEVSLSSLSQRSLWETTGRWS 87
Q+G + AS G H LP ++ + + +I + M G +V++ SLS LW+ T RW
Sbjct: 3 QVGLIYPASPGCYHLLPYTVRAMEKLVRVIDQEMQAIGGQKVNMPSLSPAELWQATNRWD 62
Query: 88 --NTELFKLKDAKKKDYCLVPTCEEEITWLMNSYLT-SYKDLPIIAYQITRKYRDEXXXX 144
EL +L+D K+YCL PT EE IT L+ S SYK LP + YQ+TRK+RDE
Sbjct: 63 LMGKELLRLRDRHGKEYCLGPTHEEAITALIASQKKLSYKQLPFLLYQVTRKFRDEPRPR 122
Query: 145 XXXXXXXEFLMKDAYSFHLNSEDAVSTFERVNATYDKIFTDIGVPFVSAVADSGDIGGDL 204
EF MKD Y+F + E A T+ V Y +F +G+PFV AD G IGG +
Sbjct: 123 FGLLRGREFYMKDMYTFDSSPEAAQQTYSLVCDAYCSLFNKLGLPFVKVQADVGTIGGTV 182
Query: 205 SKEYHFIHESGEDTLFRCNSCEAVSNIE 232
S E+ + GED L C C +N+E
Sbjct: 183 SHEFQLPVDIGEDRLAICPRCSFSANME 210
Score = 88.2 bits (217), Expect = 3e-17
Identities = 48/150 (32%), Positives = 72/150 (48%), Gaps = 14/150 (9%)
Query: 356 CAQCEEGELESAKSIEVGHTFYLGKKYSKALNATFRQQDNSDVVMEMGCYGIGVSRLIGA 415
C C+ G L K IEVGHTFYLG KYS NA F + EMGCYG+GV+R++ A
Sbjct: 219 CPACQ-GPLTKTKGIEVGHTFYLGTKYSSIFNAQFTNVCGKPTLAEMGCYGLGVTRILAA 277
Query: 416 IAQVTRDPKGLRWPSSVSPYKVSVCASDKS-------------VDQLNKVKALLDYKPYT 462
+V +RWPS ++PY+ + K D + + L +
Sbjct: 278 AIEVLSTEDCVRWPSLLAPYQACLIPPKKGSKEQAASELIGQLYDHITEAVPQLHGEVLL 337
Query: 463 DFDAKTSIGAKIQQSHALGIPIAIVVGPKS 492
D +IG +++ ++ G P I+ G ++
Sbjct: 338 DDRTHLTIGNRLKDANKFGYPFVIIAGKRA 367
>CE16514 [J] KOG2324 Prolyl-tRNA synthetase
Length = 454
Score = 118 bits (295), Expect = 3e-26
Identities = 70/219 (31%), Positives = 108/219 (48%), Gaps = 6/219 (2%)
Query: 23 HELLQQLGYVKQASSGVLHWLPLGLKTLRNIEGIIRRRMDESGAHEVSLSSLSQRSLWET 82
H +L + G++ + G LPLG + + + I+ SGA ++++ + + LWE
Sbjct: 23 HTMLVENGFILPTAKGFYSLLPLGQRVIDKLCRILDVEFQNSGALKIAMPIVGTQQLWEK 82
Query: 83 TGRWS--NTELFKLKDAKKKDYCLVPTCEEEITWLMNSYLTSYKD-LPIIAYQITRKYRD 139
TGRW E+ + D ++ CL PT EE T L+ + K P+I YQI K+RD
Sbjct: 83 TGRWEAMGPEMIRFHDRQQNHMCLQPTAEEMCTELIATLSPLKKSQFPLIVYQIGEKFRD 142
Query: 140 EXXXXXXXXXXXEFLMKDAYSFHLNSEDAVSTFERVNATYDKIFTDI---GVPFVSAVAD 196
E +FLMKD YSF ++E + T+ ++ YD+IF +I + AD
Sbjct: 143 EMNPRFGLMRGRQFLMKDMYSFSTDAETSQQTYAQIRQIYDRIFGEILQLDEDVIKVEAD 202
Query: 197 SGDIGGDLSKEYHFIHESGEDTLFRCNSCEAVSNIEKCG 235
SG GG +S EYH ED + C +C+ + E G
Sbjct: 203 SGVHGGHISHEYHLKSSLEEDFVNFCENCKNHNKFEGPG 241
Score = 74.7 bits (182), Expect = 3e-13
Identities = 56/218 (25%), Positives = 105/218 (47%), Gaps = 27/218 (12%)
Query: 293 TNEEVLAKFTENYDPMFNGIIRVMD--VRLNSRSNFP------DFPLKSYLKNNFAQL-- 342
T+++ A+ + YD +F I+++ + +++ + S ++ LKS L+ +F
Sbjct: 170 TSQQTYAQIRQIYDRIFGEILQLDEDVIKVEADSGVHGGHISHEYHLKSSLEEDFVNFCE 229
Query: 343 -----NDYSIVDVEEGEICAQCEEGELESAKSIEVGHTFYLGKKYSKALNATFRQQDNSD 397
N + + E CA+ ++ S+E+ HTF+LG KYS+AL A F+ +
Sbjct: 230 NCKNHNKFEGPGPAKCEKCAENSSKTVQKIPSVEIAHTFHLGTKYSEALGAKFQGKP--- 286
Query: 398 VVMEMGCYGIGVSRLI-GAIAQVTRDPKGLRWPSSVSPYKVSVCASDKSVDQLNKVKALL 456
++M C+GIGV+RL+ AI ++ K LR P +++P+ + + + A
Sbjct: 287 --LDMCCFGIGVTRLLPAAIDLLSVSDKALRLPRAIAPFDAVIIVKKSLMSNVIVEMASS 344
Query: 457 DYKPY------TDFDAKTSIGAKIQQSHALGIPIAIVV 488
Y D + S G +I ++ LGIP +V+
Sbjct: 345 SASRYLKGGILLDDRVEMSAGKRIHDANRLGIPFILVI 382
>YHR020w [J] KOG4163 Prolyl-tRNA synthetase
Length = 688
Score = 52.0 bits (123), Expect = 2e-06
Identities = 47/213 (22%), Positives = 94/213 (44%), Gaps = 18/213 (8%)
Query: 353 GEICAQCEEGELESAKSIEVGHTFYLGKKYSKALNATFRQQDNSD---VVMEMGCYGIGV 409
G+ CE ++ + I+ + +LG+ +SK N + SD + +G+
Sbjct: 390 GDFTTTCEGYIPQTGRGIQGATSHHLGQNFSKMFNLSVENPLGSDHPKIFAYQNSWGLS- 448
Query: 410 SRLIGAIAQVTRDPKGLRWPSSVSPYK---VSVCASDKSVDQLNK--------VKALLD- 457
+R+IG + + D KGL P VS ++ + V + K+ ++ K V++ L
Sbjct: 449 TRVIGVMVMIHSDNKGLVIPPRVSQFQSVVIPVGITKKTSEEQRKHIHETARSVESRLKK 508
Query: 458 --YKPYTDFDAKTSIGAKIQQSHALGIPIAIVVGPKSWPNVEIEVRGNFRSKNWETSYEK 515
+ + D++ + G K Q GIPI I +GPK ++ V SK + S+++
Sbjct: 509 VGIRAFGDYNDNYTPGWKFSQYELKGIPIRIELGPKDIEKNQVVVVRRNDSKKYVVSFDE 568
Query: 516 LHSQLGWEYNQESGKHFVLHENANDVVNILLKD 548
L +++ + G F + D +++ +
Sbjct: 569 LEARIPEILEEMQGDLFKKAKELFDTHRVIVNE 601
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.134 0.397
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 32,846,335
Number of Sequences: 60738
Number of extensions: 1407323
Number of successful extensions: 3590
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 3561
Number of HSP's gapped (non-prelim): 11
length of query: 549
length of database: 30,389,216
effective HSP length: 111
effective length of query: 438
effective length of database: 23,647,298
effective search space: 10357516524
effective search space used: 10357516524
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)