ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV5252 good U KOG0929 Intracellular trafficking, secretion, and vesicular transport Guanine nucleotide exchange factor
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV5252 1798423 1796582 -614
(614 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YBL060w [U] KOG0929 Guanine nucleotide exchange factor 262 1e-69
SPAC11E3.11c [U] KOG0929 Guanine nucleotide exchange factor 56 2e-07
CE12328 [U] KOG0931 Predicted guanine nucleotide exchange factor... 54 9e-07
>YBL060w [U] KOG0929 Guanine nucleotide exchange factor
Length = 687
Score = 262 bits (670), Expect = 1e-69
Identities = 186/604 (30%), Positives = 297/604 (48%), Gaps = 72/604 (11%)
Query: 48 ADQMLKQTYRPLEYTQYANWLGAKENKRVLTEFLNLLRPWPTSLYLTLSKLSHGLYLIAE 107
A ++L+ T++ + Y +YAN+LG N +VLTEF+ LL P P+SL TL LS +Y IAE
Sbjct: 102 ASEILEGTFKDVSYKEYANFLGNDNNNQVLTEFVKLLSPLPSSLLETLFNLSKSIYFIAE 161
Query: 108 ATPLDNILEELAAQWTDEHKDKVSHFQGNYRVCHIILFSLLILNSDLHN----ESFDYKF 163
A +D ILE L+ +W H + +H++ Y+ CHI+LFSLLILNSDLHN + KF
Sbjct: 162 AQNIDRILECLSIEWIACHPN--THWKSGYKSCHIVLFSLLILNSDLHNNFQVDHKKIKF 219
Query: 164 TVDQFIENTIYAIR----YELKDMQLDEKLIKDDLRGYYNQILDIPFPLC-QPRTFKEVS 218
++ FI NT+ A+R YE + E LI ++L YY + + P PLC + RT +S
Sbjct: 220 SMVAFINNTLRALREENEYEELKIYSREHLIIEELSEYYKTLNETPLPLCTESRTSINIS 279
Query: 219 NKXXXXXXXXXXXXXXXXXXXXXXXERVSFSVSRKTSLFSLRPASSLLEKXXXXXXXXXX 278
+ S ++ + S+R S+ K
Sbjct: 280 DNQSSLKRFSTLGSREFSTSNLRSVNSNSTTLYSRDGQVSVREMSAKSNK---------- 329
Query: 279 XXXXXXHVN--ATDFYVHQHEDDIFGKQNDSLWLVD-------------HTLNFKVNKST 323
H N Y+ + DD +N S W +D ++ K +
Sbjct: 330 ----NFHNNHPMDALYLKESFDDGLITENGSSWFMDDLILISKKSLPRKYSKRDKDQVAA 385
Query: 324 HSISEPCDSATHGIMKLFRRGNNLTHQQQRME----NANRKMHRARVTVQKGSLELHLTK 379
++ + + G +K + + H +R N + + R ++ V++G + + K
Sbjct: 386 PKMTSKRNKSFFGWLKPSKTTTLIEHTSRRTSLSYLNKDSEWERVKIQVKEGRIFIFKIK 445
Query: 380 SKNKLSARSLTGPNGIFG-----TSIIQRFNLFGAFAELIGETIIDHTGASKPKC----- 429
K +S + +S ++L A A ++ + II +GA K
Sbjct: 446 PDVKDIIQSSETDSATIDYFKDISSSYFAYSLLEAEAHVVQDNIIIGSGAMKSNVCNKNT 505
Query: 430 ------FQVEFADDQHHKSLI--FECSSSKQCETFVDCINFWASRITPIPDSQFEMVSNQ 481
F V F ++ + L+ F+ S ++ F+DCINFWA RI+P+P +QFE VSN
Sbjct: 506 KRKSGNFTVSFPENINGPKLVLEFQTRSVEEAHKFMDCINFWAGRISPVPLTQFEAVSNA 565
Query: 482 EYGWSGRILSEYQEDPNSFKVNPK-IKVFHWEPLYGLDSLYTNFNVEESGKLNMDSQLLH 540
EYGWS +IL+E+ +N K I V W+PL GL+ LY + ++ + + +L
Sbjct: 566 EYGWSDKILTEHA------SLNLKNIVVSEWKPLLGLELLYE--DAKDVEMVELKERLKE 617
Query: 541 WKIFVTKLAEWINEHNDIKPALIKIWTHNPN-FDLIMDNWNAKYLYLNRQYHKHNEYLKA 599
F +L WI++HN+IK L++IW+ + N F+ +M+NWN++YLY+N QY K YLKA
Sbjct: 618 LMNFTRQLGIWIDKHNEIKDKLVEIWSFDDNYFEAVMNNWNSRYLYMNNQYKKRLSYLKA 677
Query: 600 IEKS 603
++K+
Sbjct: 678 LQKA 681
>SPAC11E3.11c [U] KOG0929 Guanine nucleotide exchange factor
Length = 942
Score = 55.8 bits (133), Expect = 2e-07
Identities = 37/112 (33%), Positives = 57/112 (50%), Gaps = 5/112 (4%)
Query: 73 NKRVLTEFLNLLRPWPTSLYLTLSKLSHGLYLIAEATPLDNILEELAAQWTDEHKDKVSH 132
NK VL +++L T + +L + LY+ E LD+ L E + QW +
Sbjct: 366 NKAVLKHYISLYDFENTDILQSLRMICGNLYVHGETQELDHFLGEFSNQWC---RTNPKG 422
Query: 133 FQGNYRVCHIILFSLLILNSDLH--NESFDYKFTVDQFIENTIYAIRYELKD 182
N ++ H I FSLL+LN+DLH S + + +QF+ENT +I+ L D
Sbjct: 423 LFCNPQIVHSIAFSLLLLNTDLHIAELSASERMSRNQFVENTYRSIKQALND 474
>CE12328 [U] KOG0931 Predicted guanine nucleotide exchange factor contains
Sec7 domain
Length = 614
Score = 53.5 bits (127), Expect = 9e-07
Identities = 32/139 (23%), Positives = 68/139 (48%), Gaps = 2/139 (1%)
Query: 63 QYANWLGAKENKRVLTEFLNLLRPWPTSLYLTLSKLSHGLYLIAEATPLDNILEELAAQW 122
+Y L + + VL F L+ + + L K L EA +D I++ A +
Sbjct: 250 EYIGTLHSPYHSLVLKYFTALIDIRGLEVDVALRKAMQFFILPKEAEKIDRIIQAFALHY 309
Query: 123 TDEHKDKVSHFQGNYRVCHIILFSLLILNSDLHNESFDYKFTVDQFIENTIYAIRYELKD 182
+ + + SH++G + H++ F++++LN+DLH+ + + T F++N + ++
Sbjct: 310 SKSNPKRTSHYRGGWDTIHLLSFAIIMLNTDLHSPNVKQRMTQADFVKN--LRGQDKISG 367
Query: 183 MQLDEKLIKDDLRGYYNQI 201
+ E + + L G Y++I
Sbjct: 368 EKNGEDIDRKTLEGIYDRI 386
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.319 0.135 0.408
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 35,939,689
Number of Sequences: 60738
Number of extensions: 1518625
Number of successful extensions: 3782
Number of sequences better than 1.0e-05: 3
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 3769
Number of HSP's gapped (non-prelim): 4
length of query: 614
length of database: 30,389,216
effective HSP length: 112
effective length of query: 502
effective length of database: 23,586,560
effective search space: 11840453120
effective search space used: 11840453120
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)