ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV5374 good J KOG2438 Translation, ribosomal structure and biogenesis Glutamyl-tRNA amidotransferase subunit B
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV5374 1837058 1838674 539
(539 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YBL080c [J] KOG2438 Glutamyl-tRNA amidotransferase subunit B 623 e-178
SPAC343.13 [J] KOG2438 Glutamyl-tRNA amidotransferase subunit B 265 1e-70
At1g48520 [J] KOG2438 Glutamyl-tRNA amidotransferase subunit B 239 1e-62
Hs4758894 [J] KOG2438 Glutamyl-tRNA amidotransferase subunit B 212 1e-54
7301105 [J] KOG2438 Glutamyl-tRNA amidotransferase subunit B 209 7e-54
CE23581 [J] KOG2438 Glutamyl-tRNA amidotransferase subunit B 79 2e-14
>YBL080c [J] KOG2438 Glutamyl-tRNA amidotransferase subunit B
Length = 541
Score = 623 bits (1607), Expect = e-178
Identities = 316/535 (59%), Positives = 400/535 (74%), Gaps = 8/535 (1%)
Query: 6 RNYAKRNTGSKFKILPEYKLKCGLEIHTQLNTDNKLFSMSTNSPFADVDKPNYHTSYFDV 65
R A + G+ F+ PEY LKCGLEIHTQLNT NKLFS STNS + VD PN+HTSY+D+
Sbjct: 12 RTKAIHSHGAPFR--PEYALKCGLEIHTQLNTKNKLFSQSTNSATSLVDAPNHHTSYYDI 69
Query: 66 SIPGTQPILNLQAVLFALKLSLALRCKVSLNSHFDRKHYFYGDQPLGYQITQHYNPFSSK 125
++PGTQP+LNL+A+LFA+KLSLAL +V+ S FDRKHYFYGDQP GYQ+TQHY PF+
Sbjct: 70 ALPGTQPVLNLEAILFAMKLSLALGSQVNSISQFDRKHYFYGDQPQGYQLTQHYRPFARG 129
Query: 126 GKLRLYKDLDSIEEAFKDISVIQLQIEQDTGKSVYNSTDSN--TKIDLNRSNVPLIEMVT 183
GK+ L K+LD I+E+ K+I ++QLQIEQDTGKS Y TD + T +DLNRSNVPLIE+VT
Sbjct: 130 GKINLSKELDDIDESAKEIGILQLQIEQDTGKSHYTETDKDVITLVDLNRSNVPLIELVT 189
Query: 184 EPDFTDLKQIRAFIKKYQNLVRHLKISTGDLETGAMRVDVNLSINDYARVELKNLPNTSS 243
+PDF+D+KQ+RAFIKKYQNLVRHL IS+GDLETGAMRVDVNLSIN+YARVELKNLPNTSS
Sbjct: 190 KPDFSDIKQVRAFIKKYQNLVRHLHISSGDLETGAMRVDVNLSINEYARVELKNLPNTSS 249
Query: 244 ITNAIKYEYNRQVNMIEKGEGDQLKHTETRGWTGSETVKLRTKESIIDYRYMPDPELPNI 303
I NAIKYEY RQV +I G+ L ETRGWTGS TVKLR+KE+ IDYRYMPDPELP I
Sbjct: 250 IINAIKYEYQRQVELISVGDTSSLMEPETRGWTGSSTVKLRSKETTIDYRYMPDPELPYI 309
Query: 304 LLTNEIVEGVEKTIPTLPDDTCMMLMQEPYGLTLKDAKILTINSNGHDDLYSHDELRACY 363
L +++ GV +P LPDD +LM++PY L+LKDAKILT NSN +D+Y+H+ LR+ Y
Sbjct: 310 NLAPDVISGVRGLMPQLPDDIMRILMKKPYQLSLKDAKILTYNSN-QNDMYNHEALRSYY 368
Query: 364 FSTFDAYHSLCKKQNEEFVRKLPANWIIHELLGNLNKMEIPLIKVSHILPPEKFAELLLL 423
TF + L +++ KLP NWIIHE LG+LNK++IPL K IL P FA+ L L
Sbjct: 369 LDTFREFSKLAGERSN---AKLPTNWIIHEFLGDLNKLQIPLAKAKEILAPPVFAQFLKL 425
Query: 424 ISKKTISNASGKLLLSHILNSFKENSFVASEPINFNALIEEFELHAVSDVGSXXXXXXXX 483
+ ++ IS SGK+LL HIL +F++++ +F+ LIE+FELHA++ V
Sbjct: 426 LHEEVISATSGKMLLFHILENFEQSNCQDLSIPDFSKLIEKFELHAINQVDPQELMDLCN 485
Query: 484 XXXXXXNNNKMIEDIKSGKKKNSIKFLVGQAMRISQGRIQAQEFEITFKRLLGVE 538
++ I ++ +GKKK+S+KFL+GQ MR SQGRI+A EFE FK +L ++
Sbjct: 486 DVIAQHTDDTFIRNLVTGKKKSSLKFLIGQGMRRSQGRIKANEFEKKFKEILNIQ 540
>SPAC343.13 [J] KOG2438 Glutamyl-tRNA amidotransferase subunit B
Length = 526
Score = 265 bits (677), Expect = 1e-70
Identities = 184/540 (34%), Positives = 285/540 (52%), Gaps = 72/540 (13%)
Query: 28 GLEIHTQLNTDNKLFSMSTNSPFADVDKPNYHTSYFDVSIPGTQPILNLQAVLFALKLSL 87
GLEIH QL T KLFS + +P A +PN +YFDVS+PG+ PILN +A+ FA+K +L
Sbjct: 11 GLEIHLQLATVKKLFSPAKVNPLA---QPNTCVAYFDVSLPGSMPILNPEALKFAVKGAL 67
Query: 88 ALRCKVSLNSHFDRKHYFYGDQPLGYQITQHYNPFSSKGKLRLYKDLDSIEEAFKDISVI 147
AL C+V+ FDRKHYFY D P GYQITQ P G + L LD++ + I +
Sbjct: 68 ALNCEVANVCKFDRKHYFYPDLPAGYQITQKEIPIGRNGFIPLSAGLDNVPDT--QIPIQ 125
Query: 148 QLQIEQDTGKSVYNSTDSNTKIDLNRSNVPLIEMVTEPDFTDLKQIRAFIKKYQNLVRHL 207
+Q+EQDT KS S +D NR+ PL+E+VT+P F D+ + AF+ K Q++VR
Sbjct: 126 HIQLEQDTAKSTSAKNPSRILLDYNRAGTPLLEIVTKPCFHDVNTVSAFLFKLQSIVRFA 185
Query: 208 KISTGDLETGAMRVDVNLSI---------------------------------NDYARVE 234
IS +E G MR DVN+SI ARVE
Sbjct: 186 GISEALMENGGMRCDVNVSIAPVISSENLNELHFKNNGVISVEKSISHFPQLGTQLARVE 245
Query: 235 LKNLPNTSSITNAIKYEYNRQVNMIEKGEGDQLKHTETRGWTG--SETVKLRTKESIIDY 292
LKNL N ++ N+I++E +RQV++ G +ETRG+ +T LR K + DY
Sbjct: 246 LKNLSNVRNVVNSIRHEVDRQVSLANMGVS---WPSETRGFDDITGKTFPLRNKTTSDDY 302
Query: 293 RYMPDPELPNILLTNEIVEGVEKTIPTLPDDTCMMLMQEPYGLTLKDAK-ILTINSNGH- 350
++P+ ++P I+L+ V V K++P LPD+ L + ++ K+A+ +L++ H
Sbjct: 303 LFLPETDIPPIILSRSYVNSVLKSLPALPDELFQKLTTGSHAISHKEARTLLSVKEYYHY 362
Query: 351 -DDLYSHDELRACYFSTFDAYHSLCKKQNEEFVRKLPANWIIHELLGNLNKMEIPLIKVS 409
+ Y H D+ + + E+ +R LP WI EL+G + +++ P +
Sbjct: 363 YRNAYHH----------IDSISHVNQSLKEDALRTLP-YWITVELVGKVREID-PSPNI- 409
Query: 410 HILPPEKFAELLLLISKKTISNASGKLLLSHILNSFKENSFVASEPINFNALIEEFELHA 469
+I+PP + A+++LL+SKK ++ AS KL L ++ S +S+PI + LI+E
Sbjct: 410 NIVPPLQLADIVLLVSKKKLTAASAKLFLRSLIKS------PSSKPI--STLIQEKNFGT 461
Query: 470 VSDVGSXXXXXXXXXXXXXXNNNKMIEDIKSGKKKNSIKFLVGQAMRISQGRIQAQEFEI 529
+ D ++K E++K+G K + IK+ VG MR +G+ ++ E+
Sbjct: 462 IGD----SEKIKACVESVLSKHSKQFEELKNG-KTSLIKWFVGLTMRELRGQASPKDIEM 516
>At1g48520 [J] KOG2438 Glutamyl-tRNA amidotransferase subunit B
Length = 550
Score = 239 bits (609), Expect = 1e-62
Identities = 168/519 (32%), Positives = 267/519 (51%), Gaps = 66/519 (12%)
Query: 18 KILPEYKLKCGLEIHTQLNTDNKLFSMSTNSPFADVDKPNYHTSYFDVSIPGTQPILNLQ 77
+IL +Y+ G+E H QL+T K F +N+ + PN + +PG P+LN +
Sbjct: 65 EILRDYEAVIGIETHVQLSTLTKAFCSCSNNYGS---YPNTSICPVCMGLPGALPVLNSK 121
Query: 78 AVLFALKLSLALRCKVSLNSHFDRKHYFYGDQPLGYQITQHYNPFSSKGKLRLYKDLDSI 137
V F ++L LAL C +SL S FDRK YFY D P GYQI+Q P +S G Y D+D
Sbjct: 122 VVEFGVRLGLALNCDLSLKSKFDRKQYFYPDLPKGYQISQFDIPIASGG----YVDVDIP 177
Query: 138 EE---AFKDISVIQLQIEQDTGKSVYNSTDSNTKIDLNRSNVPLIEMVTEPDFTDLKQIR 194
E + + ++ +E+D GK +++ T +++DLNR+ VPL+E+V+EPD +
Sbjct: 178 LEFGGGHRRFGITRVHMEEDAGKLLHSDTGDYSQVDLNRAGVPLLEIVSEPDMRSGIEAA 237
Query: 195 AFIKKYQNLVRHLKISTGDLETGAMRVDVNLSINDY------ARVELKNLPNTSSITNAI 248
+ + Q + R+L +S G+++ G++R DVN+SI +VE+KNL S+I+ AI
Sbjct: 238 EYACEMQRIARYLGVSNGNMQEGSLRCDVNISIRPIGQAEFGTKVEIKNLNAFSAISRAI 297
Query: 249 KYEYNRQVNMIEKGEGDQLKHTETRGWT--GSETVKLRTKESIIDYRYMPDPELPNILLT 306
+E +RQ + +G+ DQ+ TETR W +TV +R KE + DYRY P+P+LP ++LT
Sbjct: 298 DFEISRQALLYNQGKADQIV-TETRLWEEGAQKTVTMRKKEGLADYRYFPEPDLPEVILT 356
Query: 307 NEIVEGVEKTIPTLPDDTCMMLMQEPYGLTLKDAKILTINSNGHDDLYSHDELRACYFST 366
E V+ + ++P LP+ E GL ++D L ++D A YF
Sbjct: 357 QEYVDSIRASLPELPE--AKRRRYEAMGLGMQDVLFL-----------ANDVSVAEYFD- 402
Query: 367 FDAYHSLCKKQNEEFVRKLPANWIIHELLGNLNKMEIPLIKVSHILPPEKFAELLLLISK 426
++ K E KL ANWI+ ++ L ++ + + L P++ AEL+ I
Sbjct: 403 -----AVIGKGAEV---KLAANWIMSDIAAYLKNEKLSINDIK--LTPQELAELIAAIKD 452
Query: 427 KTISNASGKLLLSHIL--NSFKENSFVASEPINFN--ALIEEFELHAVSDVGSXXXXXXX 482
TIS GK +L +L + A + + A IE+ + VS+
Sbjct: 453 GTISGKIGKEILFELLAKGGTVKGMIKAKDLVQITDPAEIEKMVIQVVSE---------- 502
Query: 483 XXXXXXXNNNKMIEDIKSGKKKNSIKFLVGQAMRISQGR 521
N K +E +SGK K + GQ M++S+G+
Sbjct: 503 --------NPKQLEQYRSGKTKLQ-GYFAGQVMKMSKGK 532
>Hs4758894 [J] KOG2438 Glutamyl-tRNA amidotransferase subunit B
Length = 557
Score = 212 bits (539), Expect = 1e-54
Identities = 155/515 (30%), Positives = 263/515 (50%), Gaps = 62/515 (12%)
Query: 28 GLEIHTQLNTDNKLFSMSTNSPFADVDKPNYHTSYFDVSIPGTQPILNLQAVLFALKLSL 87
GLEIH Q+++++KLFS S A PN S+FD S+PGT P+LN + V A+ L
Sbjct: 68 GLEIHAQISSNSKLFSGSQVRFSAP---PNSLVSFFDASLPGTLPVLNRRCVEAAVMTGL 124
Query: 88 ALRCKVSLNSHFDRKHYFYGDQPLGYQITQHYNPFSSKGKLRLYKDLDSIEEAF---KDI 144
AL C ++ S FDRKHYFY D P GYQITQ P + G L +Y +++ K +
Sbjct: 125 ALNCHINKKSLFDRKHYFYADLPAGYQITQQRLPIAVNGSL-IYGVCAGKKQSQVIPKTV 183
Query: 145 SVIQLQIEQDTGKSVYNSTDSNTKIDLNRSNVPLIEMVTEPDFTDLKQIRAFIKKYQNLV 204
+ Q+Q+EQD+GKS++++ S T IDLNR+ V L+E+V EPD + ++ +++ Q ++
Sbjct: 184 RIKQIQLEQDSGKSLHDNLRSQTLIDLNRAGVGLLEVVLEPDMSCGEEAATAVRELQLIL 243
Query: 205 RHLKISTGDLETGAMRVDVNLSIND-----YARVELKNLPNTSSITNAIKYEYNRQVNMI 259
+ L S ++ G +RVD N+S++ R E+KNL + + AI YE RQ+N +
Sbjct: 244 QALGTSQANMAEGQLRVDANISVHHPGEPLGVRTEVKNLNSIRFLAKAIDYEIQRQINEL 303
Query: 260 EKGEGDQLKHTETRGWTGSETVKLRTKESIIDYRYMPDPELPNILLTNEI---------- 309
E G G+ L T + T+ +R KE DYR+MP+P LP ++L +
Sbjct: 304 ENG-GEILNETRSFHHKLGCTMSMRDKEGKQDYRFMPEPNLPPLVLYDATSLPAGADPQQ 362
Query: 310 ---VEGVEKTIPTLPDDTCMMLMQEPYGLTLKDAKILTINSNGHDDLYSHDELRACYFST 366
++ + +T+P LP T L+Q+ YG+ L+ + L +N G
Sbjct: 363 VINIDQIRETLPELPSVTREKLVQQ-YGMLLEHSFTL-LNEVG----------------L 404
Query: 367 FDAYHSLCKKQNEEFVRKLPANWIIHELLGNLNKMEIPLIKVSHILPPEKFAELLLLISK 426
+ + ++ K+ E K +W+++ LG L + + + + + P AELL L+
Sbjct: 405 LEFFQNVIKETRAE--PKKVTSWVLNTFLGYLKQQNLAVSESP--VTPSALAELLDLLDS 460
Query: 427 KTISNASGKLLLSHILNSFKENSFVASEPINFNALIEEFELHAVSDVGSXXXXXXXXXXX 486
+TIS+++ K + + +K + ++ E +L + D G+
Sbjct: 461 RTISSSAAKQVFEEL---WKREGKTPGQ------IVSEKQLELMQDQGALEQLCHSVMEA 511
Query: 487 XXXNNNKMIEDIKSGKKKNSIKFLVGQAMRISQGR 521
+ +++ D+K+ + +I L+G + +Q R
Sbjct: 512 ----HPQVVMDVKN-RNPRAINKLIGLVRKATQSR 541
>7301105 [J] KOG2438 Glutamyl-tRNA amidotransferase subunit B
Length = 467
Score = 209 bits (533), Expect = 7e-54
Identities = 133/350 (38%), Positives = 198/350 (56%), Gaps = 26/350 (7%)
Query: 13 TGSKFKILPEYKLKC--GLEIHTQLNTDNKLFSMSTNSPFADVDKPNYHTSYFDVSIPGT 70
T K +P+ K K GLE+H Q+ + +KLFS S S A + N +YFD SIPGT
Sbjct: 11 TAPKLANVPKRKWKSVVGLEVHAQIASASKLFSGSGTSFGAPL---NSSVAYFDASIPGT 67
Query: 71 QPILNLQAVLFALKLSLALRCKVSLNSHFDRKHYFYGDQPLGYQITQHYNPFSSKGKLRL 130
P+LN + V +K SLAL C+V+ S FDRKHYFY D P GYQITQ ++ GK+
Sbjct: 68 LPVLNRKCVESGIKTSLALGCRVNEVSMFDRKHYFYADLPNGYQITQQRAALANDGKMTF 127
Query: 131 YKDLDSIEEAFKDISVIQLQIEQDTGKSVYNSTDSNTKIDLNRSNVPLIEMVTEPDFTDL 190
+ +K ++QLQ+EQD+GKS+++ + +DLNR+ +PL+E+V PD
Sbjct: 128 PVITPGKKVYYKTAKLLQLQLEQDSGKSLHDDYLKRSLVDLNRAGLPLMELVFAPDLETG 187
Query: 191 KQIRAFIKKYQNLVRHLKISTGDLETGAMRVDVNLSINDY-----ARVELKNLPNTSSIT 245
++ + +K+ ++R L+ + +E GA+RVD N+SI+ R E+KN+ + SI+
Sbjct: 188 EEAASLVKELILILRRLQTCSCKMEEGALRVDANISIHQEGDPLGVRTEVKNIGSVRSIS 247
Query: 246 NAIKYEYNRQVNMIEKGEGDQLKHTETRGWTGS--ETVKLRTKESIIDYRYMPDPELPNI 303
AI YE NRQ+ + G + ETR W TV +R KE + DYR+MP+P LP +
Sbjct: 248 QAITYEINRQLETVANG---GVITNETRNWDAENRRTVAMRDKEVLQDYRFMPEPNLPPL 304
Query: 304 --------LLTNEI--VEGVEKTIPTLPDDTCMMLMQEPYGLTLKDAKIL 343
+ T ++ V + + IP LP+DT L+ E + L + A IL
Sbjct: 305 HVNLKPGSMSTEDLLSVAALSEEIPELPEDTRQRLV-EQHNLNAETAIIL 353
>CE23581 [J] KOG2438 Glutamyl-tRNA amidotransferase subunit B
Length = 350
Score = 79.0 bits (193), Expect = 2e-14
Identities = 54/155 (34%), Positives = 86/155 (54%), Gaps = 9/155 (5%)
Query: 169 IDLNRSNVPLIEMVTEPDFTDLKQIRAFIKKYQNLVRHLKISTGDLETGAMRVDVNLSI- 227
ID NR+ L+E+VT P FT + F++ + L+ H I G+L G +RVD N+S+
Sbjct: 12 IDYNRAGCALVEIVTSPCFTTAIEAIRFVQILRLLLIHHAICDGELHRGHIRVDANVSLA 71
Query: 228 -NDY--ARVELKNLPNTSSITNAIKYEYNRQVNMIEKGEGDQLKHTETRGW-TGSETVKL 283
+D R E+KN+ + +I AI YE RQ +++ G Q ETR + TV +
Sbjct: 72 KDDVWGTRTEIKNMNSIRTIHTAINYEIARQFEILKNGGAVQ---NETRAADSEGRTVSM 128
Query: 284 RTKES-IIDYRYMPDPELPNILLTNEIVEGVEKTI 317
R KE+ DYR+M +P LP + + + ++ VE+ +
Sbjct: 129 RDKETEETDYRFMVEPNLPQLKIHGKWLKEVEEEL 163
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.135 0.383
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 31,909,445
Number of Sequences: 60738
Number of extensions: 1394784
Number of successful extensions: 3433
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 3403
Number of HSP's gapped (non-prelim): 7
length of query: 539
length of database: 30,389,216
effective HSP length: 111
effective length of query: 428
effective length of database: 23,647,298
effective search space: 10121043544
effective search space used: 10121043544
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)