ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV5520 good S KOG2318 Function unknown Uncharacterized conserved protein
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV5520 1890314 1892194 627
(627 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDR365c [S] KOG2318 Uncharacterized conserved protein 547 e-155
SPBC1734.01c [S] KOG2318 Uncharacterized conserved protein 290 3e-78
Hs18093112 [S] KOG2318 Uncharacterized conserved protein 207 4e-53
At3g01160 [S] KOG2318 Uncharacterized conserved protein 184 5e-46
7290146 [S] KOG2318 Uncharacterized conserved protein 163 8e-40
CE06006 [S] KOG2318 Uncharacterized conserved protein 84 5e-16
>YDR365c [S] KOG2318 Uncharacterized conserved protein
Length = 628
Score = 547 bits (1410), Expect = e-155
Identities = 317/646 (49%), Positives = 390/646 (60%), Gaps = 48/646 (7%)
Query: 5 GEKKEKITEDPRFSGIHNDPKFRSTKSKNFKIKLDDRFSKQDLEVRRKAKVDKYGRKIVD 64
GE +K D RF+GI++DPKF++TK+K+ KIKLD RFSK+DLEV+ K+KVDKYGRKI +
Sbjct: 3 GENPKKEGVDARFAGIYSDPKFKNTKTKDHKIKLDSRFSKKDLEVQHKSKVDKYGRKIKN 62
Query: 65 A--NKDVKDFDRYFTXXXXXXXXXXXXXXXXXXXXXXXXXVHAKSV-DRARGEPPADYVX 121
A N++++DFD+YF V+AK+V DRARGE P DYV
Sbjct: 63 AQNNRELEDFDKYFEKEAENDEDSE---------------VNAKTVVDRARGEVPDDYVS 107
Query: 122 XXXXXXXXXXXXXXXXXXXXXXXXXXXX--XKPASGNATNTLAVVNLDWDHVKSADLFVT 179
KP SG+ + LAVVNLDWDHVKS DL +T
Sbjct: 108 SSDEFTSSDSESSGESEVESEEENEVEIENAKPESGDISKNLAVVNLDWDHVKSEDLMIT 167
Query: 180 FKSFVPKGGEIQKIAIYPSEFGKERMQREEIEGPPKELFQXXXXXXXXX--------XXX 231
F SFVPKGG+I+++AIYPSEFGKERMQREE+EGPPKELFQ
Sbjct: 168 FSSFVPKGGKIERVAIYPSEFGKERMQREEVEGPPKELFQKKNKNKTSKKKKTDDSDSDM 227
Query: 232 EINVESIYEEGDAE-DYDSKAXXXXXXXXXXXXXAVVYCSDIRTAESIYQNCDGTEYEST 290
+I ++ +YEEGDA+ D DS+A A+VYCSD T+++IY NCDGTEYEST
Sbjct: 228 DIGIKDLYEEGDADKDVDSRALRQYQLDRLRYYYAIVYCSDTTTSKAIYDNCDGTEYEST 287
Query: 291 ANVFDLRYVPDGMEMDDDPRDECTSLPKNYKPAQFSTDALQHSKVKLTWDETPADRVEIS 350
AN+FDLRYVPDGM DDD RDEC+ LPKNY+P QFSTDALQHS VKLTWDETPADRVE++
Sbjct: 288 ANMFDLRYVPDGMTFDDDVRDECSILPKNYRPHQFSTDALQHSSVKLTWDETPADRVEVA 347
Query: 351 KRAFTQKEIEEMDFKAYLAXXXXXXXXXXXXQVKNKLKALVMDSVKVGQKSLFDQKEDED 410
KRAFTQKEI++MDFKAYLA + KNKLK+LV D G S + DED
Sbjct: 348 KRAFTQKEIDDMDFKAYLASDSDESDGQVDEEAKNKLKSLVGD---FGFNSKKETPNDED 404
Query: 411 DNEADMEITFTPGLDESGQQDTNDNEEESTIDXXXXXXXXXXXXXXXXXXXXXXXXXXXX 470
+ E DMEITFTP L E G + +++++EE+TI+
Sbjct: 405 E-EVDMEITFTPAL-EGGNEKSSEDKEETTIEKIRRKEKERRKARKQKVKELKQQSEKDK 462
Query: 471 XXXXXXXXDTSHSIDDD------KKKGELELLMLDENEGES--TINNKAHFNMNEILXXX 522
+ H+ D++ K K ELELLM D+++ E+ TINNKAHFNMNEIL
Sbjct: 463 KSKLKSV-NKKHTNDEEEIEKNAKSKAELELLMDDDDDTETQGTINNKAHFNMNEILRSE 521
Query: 523 XXXXXXXXXXXXXXIVDDEFTPDVNDPRFKEVFEDHDFAIDPTQPEFKKTPAMEKILKER 582
IV+D FTPD+ DPRFKEVFEDHDFAIDPTQPEFK T AM KILKER
Sbjct: 522 KEKHKKGRYQKKERIVEDTFTPDLEDPRFKEVFEDHDFAIDPTQPEFKGTQAMSKILKER 581
Query: 583 T--VXXXXXXXXXXXNHEIANTEHSKDINKIGGLVEKLKAKNKRTK 626
+ V N+ N + ++D IG LV KLK K+K +K
Sbjct: 582 SKRVKNKKRKLGGSENNMTNNADDNED---IGNLVNKLKKKSKSSK 624
>SPBC1734.01c [S] KOG2318 Uncharacterized conserved protein
Length = 682
Score = 290 bits (743), Expect = 3e-78
Identities = 191/634 (30%), Positives = 283/634 (44%), Gaps = 60/634 (9%)
Query: 1 MNSKGEKKEKITEDPRFSGIHNDPKFRSTKSKNFKIKLDDRFS--KQDLEVRRKAKVDKY 58
+N+K + + DPRF +H+DP+F K NFK+K+D+RF K+D + + A VD+Y
Sbjct: 9 INAKSRSESNVVADPRFQSVHSDPRFSRLKRGNFKVKVDERFKSLKEDKDFKTTASVDRY 68
Query: 59 GRKIVDANKDVKDFDRYFTXXXXXXXXXXXXXXXXXXXXXXXXXVHAKSVDRARGEP--- 115
GR + + +K K+ DR + + + E
Sbjct: 69 GRPL-NQDKATKEIDRLYELENEGSSSSSESSEITDNEEVASASSKSTKSEELTDEESED 127
Query: 116 -----PADYVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKPAS----GNATNTLAVVNL 166
PA +P G+ TN LAVVN+
Sbjct: 128 EEVYDPARGEGIISTSESSDESDAESETEAQPEISELAGIEPEENIPRGSETNRLAVVNM 187
Query: 167 DWDHVKSADLFVTFKSFVPKGGEIQKIAIYPSEFGKERMQREEIEGPPKELFQXXXXXXX 226
DWD++++ DLFV SF P GG++ K++IYPSEFGK RM E ++GPP+++F
Sbjct: 188 DWDNLQAVDLFVALSSFCPPGGKLLKVSIYPSEFGKSRMAAEHVQGPPRDIFTPADNQPS 247
Query: 227 XXXXXEI------NVESIYEEGDA-------EDYDSKAXXXXXXXXXXXXXAVVYCSDIR 273
E N ES +E DA ++D AVV C +R
Sbjct: 248 SAELHEAQKFGFDNNESDQDEEDALIEEDLGNEFDMVKLRQYQLERLRYYYAVVECDSVR 307
Query: 274 TAESIYQNCDGTEYESTANVFDLRYVPDGMEMDDD-PRDECTSLPKNYKPAQFSTDALQH 332
TA+ IY+ CDG EYE++AN++DLR++PD + DDD R+ CT P+ Y+P F TDALQH
Sbjct: 308 TAKVIYETCDGAEYETSANIYDLRFIPDDVTFDDDESREVCTKAPEKYEPRDFVTDALQH 367
Query: 333 SKVKLTWDETPADRVEISKRAFTQKEIEEMDFKAYLAXXXXXXXXXXXXQVK-NKLKALV 391
SKVKL+WD R ++ K+AFT ++IE++DF AY+A + + KL +
Sbjct: 368 SKVKLSWDAEDPHRKDLIKKAFTSQDIEDLDFSAYIASSESEDEDVDVIRSRYQKLLSGD 427
Query: 392 MDSVKVGQKSLFDQKEDEDDNEADMEITFTPGLDESGQQDTNDNEEESTIDXXXXXXXXX 451
D + D + E N +ME+TFT G D ++++ ++E+TI+
Sbjct: 428 ADDFQANSNPFEDDDKLEGAN-GEMEVTFTSGFDVDNNANSSE-KDETTIEKYKRKAAER 485
Query: 452 XXXXXXXXXXXXXXXXXXXXXXXXXXXDTSHSIDDD-------KKKG------------- 491
D D D KKG
Sbjct: 486 KQRRKELRQLKKTKDDEGEGSDVDLGFDDPFFKDKDASRNNKKNKKGKHTQIEDPTAASK 545
Query: 492 -ELE-LLMLDENEGESTINNKAHFNMNEILXXXXXXXXXXXXXXXXXI--VDDEFTPDVN 547
ELE L+ DEN+ E HF+M IL + + + F DV+
Sbjct: 546 EELENLVREDENDSEQL----DHFDMKSILKAEKFKKNRKLKKKASNLEGLQEGFEADVS 601
Query: 548 DPRFKEVFEDHDFAIDPTQPEFKKTPAMEKILKE 581
DPRF ++ +H+FA+DPT P FK+T +EKI+ E
Sbjct: 602 DPRFAALYTNHNFALDPTNPHFKRTKTVEKIMDE 635
>Hs18093112 [S] KOG2318 Uncharacterized conserved protein
Length = 851
Score = 207 bits (527), Expect = 4e-53
Identities = 142/482 (29%), Positives = 224/482 (46%), Gaps = 80/482 (16%)
Query: 152 PASGNATNTLAVVNLDWDHVKSADLFVTFKSFVPKGGEIQKIAIYPSEFGKERMQREEIE 211
P + T LAV N+DWD +K+ DL F SF PKGG I + IYPSEFGKERM+ E+++
Sbjct: 341 PRADEITRRLAVCNMDWDRLKAKDLLALFNSFKPKGGVIFSVKIYPSEFGKERMKEEQVQ 400
Query: 212 GPPKELFQXXXXXXXXXXXXEINVESIYEEGDAEDYDSK-AXXXXXXXXXXXXXAVVYCS 270
GP + + SI E+ +D+ S+ AVV C
Sbjct: 401 GP-------------------VELLSIPEDAPEKDWTSREKLRDYQFKRLKYYYAVVDCD 441
Query: 271 DIRTAESIYQNCDGTEYESTANVFDLRYVPDGMEMDDDPRDECTSLPKN-YKPAQFSTDA 329
TA IY++CDG E+ES+ + DLR++PD + DD+P+D + + YKP F++ A
Sbjct: 442 SPETASKIYEDCDGLEFESSCSFIDLRFIPDDITFDDEPKDVASEVNLTAYKPKYFTSAA 501
Query: 330 LQHSKVKLTWDETPADRVEISKRAFTQKEIEEMDFKAYLAXXXXXXXXXXXXQVKNKLKA 389
+ S V++TWDET +R+ + R F ++E+ +MDF+AYLA +++ +L+
Sbjct: 502 MGTSTVEITWDETDHERITMLNRKFKKEELLDMDFQAYLA-----SSSEDEEEIEEELQG 556
Query: 390 LVMDSVKVGQKSLFDQKEDED--------------------DNEADMEITFTPGLDESGQ 429
+V+ K+ QK+DE+ +N+ +MEI + PGL ES +
Sbjct: 557 DDGVNVEEDGKTKKSQKDDEEQIAKYRQLLQVIQEKEKKGKENDMEMEIKWVPGLKESAE 616
Query: 430 QDTNDNEE--------ESTIDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDTS 481
+ + E E ++ +
Sbjct: 617 EMVKNKLEGKDKLTPWEQFLEKKKEKKRLKRKQKALAEEASEEELPSDVDLNDPYFAEEV 676
Query: 482 HSIDDDKK-----------KGELEL---------LMLDENEGESTINNKAHFNMNEIL-X 520
I +KK + E+E+ LM+DE+E ++K HFN N+I+
Sbjct: 677 KQIGINKKSVKSAKDGTSPEEEIEIERQKAEMALLMMDEDE-----DSKKHFNYNKIVEH 731
Query: 521 XXXXXXXXXXXXXXXXIVDDEFTPDVNDPRFKEVFEDHDFAIDPTQPEFKKTPAMEKILK 580
+++D+F +VND RF+ ++ H F +DP+ P FKKT AMEKIL+
Sbjct: 732 QNLSKKKKKQLMKKKELIEDDFEVNVNDARFQAMYTSHLFNLDPSDPNFKKTKAMEKILE 791
Query: 581 ER 582
E+
Sbjct: 792 EK 793
>At3g01160 [S] KOG2318 Uncharacterized conserved protein
Length = 713
Score = 184 bits (466), Expect = 5e-46
Identities = 130/467 (27%), Positives = 200/467 (41%), Gaps = 59/467 (12%)
Query: 152 PASGNATNTLAVVNLDWDHVKSADLFVTFKSFVPKGGEIQKIAIYPSEFGKERMQREEIE 211
P T+ LA+VN+DW HV + DL+V SF+PK G I +A+YPSEFG ERM+ EEI
Sbjct: 193 PLIQEETHRLAIVNMDWRHVSAKDLYVVLNSFLPKDGRILSVAVYPSEFGLERMKEEEIH 252
Query: 212 GPPKELFQXXXXXXXXXXXXEINVESIYEEGDAEDYDSKAXXXXXXXXXXXXXAVVYCSD 271
GP + + + + EE + ED ++ AV C
Sbjct: 253 GPVID----------GDKKNDASDDEDEEEEEDEDVINQKLRAYEISRLKYYFAVAECDS 302
Query: 272 IRTAESIYQNCDGTEYESTANVFDLRYVPDGMEMDDDPRDECTSLPKNYKPAQFSTDALQ 331
TA+ +Y++CDG E+E ++N DLR++PD ME PRD + P Y+ F + ALQ
Sbjct: 303 SATADYLYKSCDGIEFERSSNKLDLRFIPDSMEFKHPPRDIASEAPAGYEGLDFQSRALQ 362
Query: 332 HSKVKLTWDETPADRVEISKRAFTQKEIEEMDFKAYLAXXXXXX------XXXXXXQVKN 385
SKV L+WDE R++ + F +++ ++ K +LA Q K
Sbjct: 363 MSKVNLSWDEDEPHRIKTLNQKFNPEQLANLEMKEFLASDESDSDEEDDLGNEVINQSKK 422
Query: 386 KLKALVMDSVKVGQKSLFDQKEDEDDNEADMEITFTPGLDESGQQ--DTNDNEEESTIDX 443
K K + + + K+ E++N+ DME+TF GL++ ++ DN+ ES +
Sbjct: 423 KDKKKDKYRALIEAEDVDSDKDLEEENDQDMEVTFNTGLEDLSKEILKKKDNQSESVWET 482
Query: 444 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDTSHSIDD----------DKKKG-- 491
DD KK+G
Sbjct: 483 YLRQRREKKRARKNKQKDDDSSPDDDDDYNIDRKAVKDDGDDDFFMEEPPLKKKKKEGKT 542
Query: 492 -------------ELELLMLDENEGESTINNKAHFNMNEILXXXXXXXXXXXXXXXXXIV 538
ELELL+ DEN G+ N +N+ I
Sbjct: 543 KKEEVAAEEKSRAELELLLADENAGDG--NGLKGYNIKR-----------KAKKGKTDIS 589
Query: 539 DDEF-TPDVNDPRFKEVFEDHDFAIDPTQPEFKK--TPAMEKILKER 582
+D+ +++DPRF +F +A+DPT P+FK+ T A + LK++
Sbjct: 590 EDKIPAAELDDPRFSALFSSPYYALDPTDPQFKRSATYARQLALKQK 636
>7290146 [S] KOG2318 Uncharacterized conserved protein
Length = 771
Score = 163 bits (412), Expect = 8e-40
Identities = 92/216 (42%), Positives = 119/216 (54%), Gaps = 13/216 (6%)
Query: 157 ATNTLAVVNLDWDHVKSADLFVTFKSFVPKGGEIQKIAIYPSEFGKERMQREEIEGPPKE 216
+T LAV N+DWD +++ DL V SF+P GG I + IYPSEFGK RM EE+ G PKE
Sbjct: 223 STRRLAVCNMDWDRIRAEDLMVMLSSFLPPGGSILSVKIYPSEFGKARMAEEEVHG-PKE 281
Query: 217 LFQXXXXXXXXXXXXEINVESIYEEGDAEDYDSKAXXXXXXXXXXXXXAVVYCSDIRTAE 276
L + +S EEG EDY + AVV C I TA+
Sbjct: 282 LVERVEPEDDSDEELVRQQDSDAEEG--EDYHMEKLRQYQLNRLRYYYAVVECDSIDTAD 339
Query: 277 SIYQNCDGTEYESTANVFDLRYVPDGMEMDDD-PRDECTSLP--KNYKPAQFSTDALQHS 333
+Y+ CDG EYES+A DLR++PD ++D P DEC LP +YKP QF+T ALQ +
Sbjct: 340 KVYKECDGIEYESSATRVDLRFIPDDTSFEEDTPSDECFELPDASSYKPRQFTTTALQQA 399
Query: 334 KVKLTWDETPADRVEISKR-------AFTQKEIEEM 362
KV LTWDET DR E+ + T KE+ ++
Sbjct: 400 KVDLTWDETALDRRELGDKLSSGQVDKLTDKELRQI 435
Score = 55.5 bits (132), Expect = 2e-07
Identities = 41/160 (25%), Positives = 69/160 (42%), Gaps = 21/160 (13%)
Query: 479 DTSHSIDDDKKKGELELLMLDENEGESTINNKAHFNMNEILXXXXXXXXXXXXXXXXXI- 537
D ++ +++ ELELL LD+ +G + K HF++ +IL +
Sbjct: 606 DGEEDVEAKRQEKELELL-LDDGDGR---DEKQHFSLTKILKKEEQEASGSKRKRRQKLK 661
Query: 538 -----------VDDEFTPDVNDPRFKEVFEDHDFAIDPTQPEFKKTPAMEKILKERTVXX 586
+DD+F ++ND RF V++ H++ IDPT +K T M++I+ E+
Sbjct: 662 KSKNQPQNAEKLDDDFQVNLNDNRFSAVYKSHEYNIDPTHSHYKPTKGMQQIIGEKLKRR 721
Query: 587 XXXXXXXXXNHEIANTE-----HSKDINKIGGLVEKLKAK 621
N + E SK + LV+ LK K
Sbjct: 722 KEQGEGGAANGDADGDEQVAPKRSKQQLEQNALVKSLKRK 761
>CE06006 [S] KOG2318 Uncharacterized conserved protein
Length = 608
Score = 84.3 bits (207), Expect = 5e-16
Identities = 79/329 (24%), Positives = 128/329 (38%), Gaps = 61/329 (18%)
Query: 158 TNTLAVVNLDWDHVKSADLFVTFKSFV-------------------------------PK 186
+N LAV NL+WD + D+ + KSFV P+
Sbjct: 181 SNRLAVCNLEWDTMNCEDILMLVKSFVENRKKFFKNMLKKGENYTFIETVDFDERIEKPQ 240
Query: 187 GGEIQKIAIYPSEFGKERMQREEIEGPPKELFQXXXXXXXXXXXXEINVESIYEEGDAED 246
GG + + IY S+FGK ++ +EE GP +L + VE Y+E + +D
Sbjct: 241 GGSVISVGIYLSDFGKGQLSKEEKTGPLLKLAKP--------------VEE-YKEDEMDD 285
Query: 247 YDSKAXXXXXXXXXXXXXAVVYCSDIRTAESIYQNCDGTEYESTANVFDLRYVPDGMEMD 306
A +V+ I +A ++Y+ CDG ++E T D+R++PD M+ +
Sbjct: 286 ETRTAVREYLVNKLKYYYSVITFDSIPSAVAVYEECDGFQFEETGLKMDMRFIPDDMDFE 345
Query: 307 DDPRDECTSLP----KNYKPAQFSTDALQHSKVKLTWDETPADRVEISKRAFTQKE---- 358
+D E + YK + S A+ + K+ WDE R + AF E
Sbjct: 346 EDRVKEFLNAEDVNLTKYKAKKKSKSAIISTGAKILWDEDDPQRKKKFLEAFNGDEDAGK 405
Query: 359 ---IEEMDFKAYLAXXXXXXXXXXXXQVKNKLKALVMDSVKVGQKSLFDQ--KEDEDDNE 413
++ + A + K+KL K G S + K D+DD E
Sbjct: 406 DLIVDSDESDGDEANRKTLMALLNTDERKSKLDVDWEGEEKNGSDSSDGEYVKVDDDDEE 465
Query: 414 ADMEITFTPGLDESGQQDTNDNEEESTID 442
++ G D+ +D + EE D
Sbjct: 466 IGVKQKKKKGSDD--DEDEKEEVEEKKED 492
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.311 0.131 0.366
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 30,067,222
Number of Sequences: 60738
Number of extensions: 1088856
Number of successful extensions: 2889
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 2836
Number of HSP's gapped (non-prelim): 27
length of query: 627
length of database: 30,389,216
effective HSP length: 113
effective length of query: 514
effective length of database: 23,525,822
effective search space: 12092272508
effective search space used: 12092272508
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)