ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV5572 good R KOG3034 General function prediction only Isoamyl acetate-hydrolyzing esterase and related enzymes
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactV5572 1912931  1913746 272  
         (272 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YDR361c [R] KOG3034 Isoamyl acetate-hydrolyzing esterase and rel...   351   5e-97
SPCC613.08 [R] KOG3034 Isoamyl acetate-hydrolyzing esterase and ...   126   4e-29
Hs7706581 [R] KOG3034 Isoamyl acetate-hydrolyzing esterase and r...    49   1e-05

>YDR361c [R] KOG3034 Isoamyl acetate-hydrolyzing esterase and related
           enzymes
          Length = 283

 Score =  351 bits (901), Expect = 5e-97
 Identities = 184/285 (64%), Positives = 219/285 (76%), Gaps = 15/285 (5%)

Query: 1   MVQVVKLSALS--KKRAVDEE-----SDLDISSTDSNISDXXXXXXXXXXXXXDFDFFNG 53
           MVQ +KL+ L   K++ V+EE     S++DISSTDS   +             DFDFF G
Sbjct: 1   MVQAIKLNDLKNRKRKNVEEENGSDESEIDISSTDSENEEEQNGEEEIVNI--DFDFFGG 58

Query: 54  NPDVDFHALKNLMRQLFGPQESNRIQLSELANLILDSPTTTIKTDGKESDPYCFLSFIDY 113
           NP+VDFHALKNL+RQLFGPQES RIQLS LA+LIL SPTTTIKTDGKESDPYCFLSF+D+
Sbjct: 59  NPEVDFHALKNLLRQLFGPQESTRIQLSSLADLILGSPTTTIKTDGKESDPYCFLSFVDF 118

Query: 114 KANKDSDYAKYLRKVDPKLATFLQT-IDQSEKTCSLVLSERLINMPPEVVPPLYTITLED 172
           KAN  SDY KYL+KVD +L+TF +T ID   K C+LVLSERLINMPPEVVPPLY ITLED
Sbjct: 119 KANHLSDYVKYLQKVDMRLSTFFKTMIDSGNKNCALVLSERLINMPPEVVPPLYKITLED 178

Query: 173 VKLALGDDKNYDFYLIVSRKYEVNFDMDED----DLTSNQAVRKKVR-QTNEVDYFHEED 227
           V  ALGDDK+YDFY+IV+RKYEVNFD D+D        N+  R K R + +EVDYFHEED
Sbjct: 179 VATALGDDKHYDFYIIVTRKYEVNFDTDDDTDSGKRNKNKDERSKKRVKADEVDYFHEED 238

Query: 228 RFLEKLAKVHVDSEARKGLITSYMVLDHAGLIKSIEDLDAAIKTW 272
           RF EK AK+H +SEA+KG+I+SYM+LDH GL+KSI++L+  I TW
Sbjct: 239 RFFEKYAKIHFESEAKKGVISSYMILDHEGLVKSIDELETEISTW 283


>SPCC613.08 [R] KOG3034 Isoamyl acetate-hydrolyzing esterase and related
           enzymes
          Length = 325

 Score =  126 bits (316), Expect = 4e-29
 Identities = 76/203 (37%), Positives = 117/203 (57%), Gaps = 17/203 (8%)

Query: 47  DFDFFNGNPDVDFHALKNLMRQLFGPQESNRIQLSELANLILDSPT--TTIKTDGKESDP 104
           DF+FF+  P +DFHA KNL++QL G   +N + LS LA+LIL  P   +T+K DG  SDP
Sbjct: 72  DFEFFDPQP-IDFHAFKNLLKQLLGYDHTN-VNLSALADLILSQPLLGSTVKVDGNNSDP 129

Query: 105 YCFLSFIDYKANKDSDYAKYLR--------KVDPKLATFLQTI--DQSEKTCSLVLSERL 154
           Y  LS I+    +D    K L         K + +L   LQ +    S     L+++ERL
Sbjct: 130 YAMLSVINLNTRRDEPVIKQLTSYIISRLAKSNSRLENELQKLLEPNSGSQVGLIVNERL 189

Query: 155 INMPPEVVPPLYTITLEDVKLALGDDKNYDF--YLIVSRKY-EVNFDMDEDDLTSNQAVR 211
           INMP +V+PP+Y + LE+++ A+ +++ Y+F  YL++SR Y E+   + +D+  S +  +
Sbjct: 190 INMPVQVIPPMYNMLLEEMQWAINENEPYNFTHYLLLSRTYTEIESKLMDDERPSKKGKK 249

Query: 212 KKVRQTNEVDYFHEEDRFLEKLA 234
            K     EV +FH ED    ++A
Sbjct: 250 SKKTSGEEVMFFHPEDEQFREVA 272


>Hs7706581 [R] KOG3034 Isoamyl acetate-hydrolyzing esterase and related
           enzymes
          Length = 322

 Score = 48.5 bits (114), Expect = 1e-05
 Identities = 64/307 (20%), Positives = 133/307 (42%), Gaps = 56/307 (18%)

Query: 7   LSALSKKRAV--------------DEESDLDISSTDSNISDXXXXXXXXXXXXXD----- 47
           +++ SK+RAV              DEE + ++ + D +  D             +     
Sbjct: 1   MASRSKRRAVESGVPQPPDPPVQRDEEEEKEVENEDEDDDDSDKEKDEEDEVIDEEVNIE 60

Query: 48  FDFFNGNPDVDFHALKNLMRQLFGPQESNRIQLSEL-------ANLILDSPTTTIKTDGK 100
           F+ ++ + D D+  +K L++QLF     N  +L++L        ++I  +  +    D  
Sbjct: 61  FEAYSLS-DNDYDGIKKLLQQLFLKAPVNTAELTDLLIQQNHIGSVIKQTDVSEDSNDDM 119

Query: 101 ESDP-YCFLSFIDYKANKDSDYAKYLRKVDPKLA------TFLQTIDQ----SEKTCSLV 149
           + D  + F+S ++    K +   + ++++  +        + ++ +D+    + K   L+
Sbjct: 120 DEDEVFGFISLLNLTERKGTQCVEQIQELVLRFCEKNCEKSMVEQLDKFLNDTTKPVGLL 179

Query: 150 LSERLINMPPEVVPPLYTITLEDVKLALGDDK---NYDFYLIVSRKYEVNFDMDEDDLTS 206
           LSER IN+PP++  P+Y    +++  A   +K      FYL++S+ +      +     S
Sbjct: 180 LSERFINVPPQIALPMYQQLQKELAGAHRTNKPCGKCYFYLLISKTFVEAGKNNSKKKPS 239

Query: 207 NQAVRKKVRQTNEVDYFHEED------------RFLEKLAKVHVDSEARKGLITSYMVLD 254
           N+     +    E ++F+EE             R LE +   H  +   +G +T+ + L 
Sbjct: 240 NKKKAALMFANAEEEFFYEEQGKPEVLGGPDTRRGLEPVPIQH--NGGSRGQVTALVSL- 296

Query: 255 HAGLIKS 261
            AGLI+S
Sbjct: 297 KAGLIQS 303


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.316    0.134    0.374 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,203,387
Number of Sequences: 60738
Number of extensions: 631327
Number of successful extensions: 1898
Number of sequences better than 1.0e-05: 3
Number of HSP's better than  0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 1891
Number of HSP's gapped (non-prelim): 3
length of query: 272
length of database: 30,389,216
effective HSP length: 105
effective length of query: 167
effective length of database: 24,011,726
effective search space: 4009958242
effective search space used: 4009958242
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)