ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV5600 good A KOG2793 RNA processing and modification Putative N2,N2-dimethylguanosine tRNA methyltransferase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV5600 1924305 1923106 -400
(400 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YBR271w [A] KOG2793 Putative N2N2-dimethylguanosine tRNA methylt... 331 1e-90
SPCC338.11c [A] KOG2793 Putative N2N2-dimethylguanosine tRNA met... 105 1e-22
CE26910 [A] KOG2793 Putative N2N2-dimethylguanosine tRNA methylt... 64 5e-10
YLR285w [R] KOG2920 Predicted methyltransferase 57 6e-08
YNL024c [A] KOG2793 Putative N2N2-dimethylguanosine tRNA methylt... 56 8e-08
CE00292 [R] KOG2497 Predicted methyltransferase 53 9e-07
Hs20562158 [R] KOG2497 Predicted methyltransferase 52 2e-06
At5g44170 [A] KOG2793 Putative N2N2-dimethylguanosine tRNA methy... 51 3e-06
Hs17475528 [A] KOG2793 Putative N2N2-dimethylguanosine tRNA meth... 51 3e-06
At5g49560 [A] KOG2793 Putative N2N2-dimethylguanosine tRNA methy... 51 3e-06
SPAC23C4.06c [A] KOG2793 Putative N2N2-dimethylguanosine tRNA me... 50 6e-06
>YBR271w [A] KOG2793 Putative N2N2-dimethylguanosine tRNA methyltransferase
Length = 419
Score = 331 bits (848), Expect = 1e-90
Identities = 198/423 (46%), Positives = 266/423 (62%), Gaps = 37/423 (8%)
Query: 5 FDPLDFISSNAV-TKSNNIDFLERADQDTNNVQPLSDDFDVDAWPSEGE-VPIQPLDLPC 62
FDPLD + + + ++ + ER +D + Q VD S+ + I LDLP
Sbjct: 2 FDPLDLYTPDDIQVEALQFNLAEREPKDPCSPQRDEILTAVDEEESDDDDTIIDNLDLPS 61
Query: 63 VQYAAPDVILTVLKLLQPATQVNFKSAEGKNDETWEKMCNEKGVTESMLQLTLKYY-EQW 121
V+YA P+VIL +L LL+P QVNF GKN E +C G+ +L+ L +Y E+W
Sbjct: 62 VKYAPPEVILCILILLKPDRQVNFNQETGKNKSVLE-VCKSHGLEPDLLKRLLTWYTEEW 120
Query: 122 INNLLRSYSDLCTKIPALYHNRSSESELLNYFTTILSKYETS---SNELANQILQQASSR 178
N L S +C KIP L S E LL Y+T++L KY S + E+ ++L++ SSR
Sbjct: 121 PNKRLNSLEKICNKIPMLRFTVSKEL-LLGYYTSVLKKYNNSCGLNEEIIQELLKELSSR 179
Query: 179 ISERCGRTAQPSMTRTFKLEGLDTSIKLYEPALTSDNLGLKTWGSSLMLSQKII------ 232
ISE CGRTAQPS+ R F+L L TSI L+EP+LT+DNLG KTWGSSL+LSQ ++
Sbjct: 180 ISENCGRTAQPSIVRYFELRNLSTSIPLHEPSLTADNLGWKTWGSSLILSQLVVDHLDYL 239
Query: 233 -----GIPAGK-----RVLELGSGTGLVGISYAL-------THSINGDSVIFLTDLPEIL 275
+ A +VLELG+GTGLVG+S+AL T +I IF+TDLPEI+
Sbjct: 240 HTTNVNMLANSDIKQIKVLELGAGTGLVGLSWALKWKELYGTENIE----IFVTDLPEIV 295
Query: 276 PNLQYNVRLNNLND-VTADVLDWTDPLSFTEKYGNE-PFDTIVIADPIYSPQHPIWLVDM 333
NL+ NV LNNL D V A++LDWT+P F +K+G+E FD I+IADPIYSPQHP W+V+M
Sbjct: 296 TNLKKNVSLNNLGDFVQAEILDWTNPHDFIDKFGHENEFDVILIADPIYSPQHPEWVVNM 355
Query: 334 IVKFLSENGKVYLQIPIRPKYESERELLWKLLEENDLNVIAEVQEKGKDDWGDVDYLYKE 393
I KFL+ +G +L+IP+R KY ERE+L LL+E+DL V+ E +G DDWG V YLY++
Sbjct: 356 ISKFLAASGTCHLEIPLRAKYAKEREVLKLLLKESDLKVVEERHSEGVDDWGAVKYLYRQ 415
Query: 394 ITR 396
I R
Sbjct: 416 IVR 418
>SPCC338.11c [A] KOG2793 Putative N2N2-dimethylguanosine tRNA methyltransferase
Length = 303
Score = 105 bits (262), Expect = 1e-22
Identities = 89/285 (31%), Positives = 140/285 (48%), Gaps = 36/285 (12%)
Query: 128 SYS---DLCTKIPALYH----NRSSESELLNYFTTILS---KYETSSNELANQILQQASS 177
SYS D+ KIP H + E +LN+ +L+ ++ T E + ++
Sbjct: 16 SYSQIKDVLDKIPTGEHLWDLPKYEEKIILNWLIKLLATNLEWITVEEE-RDYLVSTICE 74
Query: 178 RISERCGRTAQPSMTRTFKLEGLDTSIKLYEPALTSDNLGLKTWGSSLMLS---QKIIGI 234
RI+ER GR A P+ R F L S+ L EP +T + LG KTWGS+ +LS K +
Sbjct: 75 RIAERSGRLAAPTRKREFSLSN-GVSVVLREPTMTYNTLGFKTWGSAPLLSANLPKWEDL 133
Query: 235 PAGKRVLELGSGTGLVGISYALTHSINGDSVIFLTDLPEILPNLQYNVRLNN------LN 288
LELG+GTGLVGIS A+ + TDLP+I+ N+QYNV N+
Sbjct: 134 SNSINALELGAGTGLVGISAAIQLGWQ----VVCTDLPDIVENMQYNVDYNSELIQQYAG 189
Query: 289 DVTADVLDWTDPLSFTEK--YGNEPFDTIVIADPIYSPQHPIWLVDMIVKFLSENGKVYL 346
V+ VLDW +P + + +PF I+ +D IY + + K+L+++G V
Sbjct: 190 SVSCHVLDWMNPPDDDNRPSWLIKPFQRIIASDCIYETHFGELAIALFRKYLAKDGIVIT 249
Query: 347 QIPIRPKYESERELLWKLLE----ENDLNVIAEVQEKGKDDWGDV 387
+ P+R + E + K ++ E + +E G++D+G +
Sbjct: 250 EYPLRETHLEEIGVFEKGMDAAGFERQMG-----EEIGEEDFGSL 289
>CE26910 [A] KOG2793 Putative N2N2-dimethylguanosine tRNA methyltransferase
Length = 282
Score = 63.5 bits (153), Expect = 5e-10
Identities = 58/190 (30%), Positives = 85/190 (44%), Gaps = 26/190 (13%)
Query: 116 KYYEQWINNLLRSYSDLCTK-IPALYHNRSSESELLNYFTTILSKYETSSNELANQILQQ 174
K +E W N ++ SD T I YH ES F+ + S + T S + + Q
Sbjct: 19 KCHENWKNR--KTKSDKATVFIKGEYHKTLKESRPEQLFSAVYSSWRTDSGVIFS--FSQ 74
Query: 175 ASSRISERCGRTAQPSMTRTFKLEGLDTSIKLYEPALTSDNLGLKTWGSSLMLSQKIIGI 234
++ SE R + +Y+ +T ++G W S+LM
Sbjct: 75 SNMAESEE--------YARVISFNNDHDELIIYQETVT--DVGGVIWDSALMTIHYFFKY 124
Query: 235 PA---GKRVLELGSGTGLVGISYALTHSINGDSVIFLTDLPEILPNLQYNVRLNNL---N 288
P GK+VLELGSGTG+ GI+ A ++ D + +TDLPE L ++ NV N N
Sbjct: 125 PKPFEGKKVLELGSGTGVGGIALA---ALGAD--VIITDLPERLALIEKNVEANRKLTGN 179
Query: 289 DVTADVLDWT 298
+ VLDWT
Sbjct: 180 RIKVQVLDWT 189
>YLR285w [R] KOG2920 Predicted methyltransferase
Length = 261
Score = 56.6 bits (135), Expect = 6e-08
Identities = 53/203 (26%), Positives = 98/203 (48%), Gaps = 23/203 (11%)
Query: 191 MTRTFKLEGLDTSIKLYEPALTSDNLGLKTWGSSLMLSQKIIGIPA---GKRVLELGSGT 247
+T+ K + D ++L TS G W + + + + P GK VLELG+
Sbjct: 37 ITKESKSDVKDIKLRLVG---TSPLWGHLLWNAGIYTANHLDSHPELIKGKTVLELGAAA 93
Query: 248 GLVGISYALTHSINGDSVIFLTDL--PEILPNLQYNVRLN---NLNDVTADVLDWTDP-- 300
L + AL NG ++ TD P+++ N+ YN++ N + N+V+ + W +
Sbjct: 94 ALPSVICAL----NGAQMVVSTDYPDPDLMQNIDYNIKSNVPEDFNNVSTEGYIWGNDYS 149
Query: 301 --LSFTEKYGNE--PFDTIVIADPIYSPQHPIWLVDMIVKFLSENGKVYLQI-PIRPKYE 355
L+ EK GN FD I+++D +++ L+ L+E G+ + P RPK
Sbjct: 150 PLLAHIEKIGNNNGKFDLIILSDLVFNHTEHHKLLQTTKDLLAEKGQALVVFSPHRPKL- 208
Query: 356 SERELLWKLLEENDLNVIAEVQE 378
E++L + L +N+ +++ ++ E
Sbjct: 209 LEKDLEFFELAKNEFHLVPQLIE 231
>YNL024c [A] KOG2793 Putative N2N2-dimethylguanosine tRNA methyltransferase
Length = 246
Score = 56.2 bits (134), Expect = 8e-08
Identities = 36/105 (34%), Positives = 63/105 (59%), Gaps = 8/105 (7%)
Query: 226 MLSQKIIGIPAGKRVLELGSGTGLVGISYAL--THSINGDSVIFLTDLPEILPNLQYNVR 283
+LS+ + G K+VLELGSGTGLVG+ L ++ + + +++TD+ +++P L+ N+
Sbjct: 69 LLSKTVNGTKQFKKVLELGSGTGLVGLCVGLLEKNTFHDGTKVYVTDIDKLIPLLKRNIE 128
Query: 284 LNNLN-DVTADVLDWTDPLS--FTEKYG---NEPFDTIVIADPIY 322
L+ + +V A L W +PLS F+ + G D ++ AD +Y
Sbjct: 129 LDEVQYEVLARELWWGEPLSADFSPQEGAMQANNVDLVLAADCVY 173
>CE00292 [R] KOG2497 Predicted methyltransferase
Length = 409
Score = 52.8 bits (125), Expect = 9e-07
Identities = 64/262 (24%), Positives = 113/262 (42%), Gaps = 30/262 (11%)
Query: 150 LNYFTTILSKYETSSNELANQILQQASSRISERCGRTAQPSMTRTFKLEGLDTSIKLYEP 209
+N ++S+ + ++ + + + + S + + G + R + +G D + +
Sbjct: 137 INVLKNLISELQANNVVVPDSMFDRLGSLMGMKEGEYVE----RVYLTDGGDKVLAKFSE 192
Query: 210 A---LTSDNLGLKTWGSSLMLSQKIIGIPAG-KRVLELGSGTGLVGISYALTHSINGDSV 265
A L+ GL W +S L+ IP+ R+LELGSG G+ GI+ A + D
Sbjct: 193 AVNQLSMGTTGLSVWQASCDLANLFRLIPSEYNRILELGSGCGVSGIAIAKMN----DCC 248
Query: 266 IFLTDLPE-ILPNLQYNVRLNNL-----------NDVTADVLDWTDPLSFTEKYGNEPFD 313
+ LTD + +L L+ N+ N+L N T L+W D F+E EP D
Sbjct: 249 VTLTDYDDNVLNLLEENIAKNDLKSDTEDSSANNNQATVRSLNWCD-FDFSE--WKEPTD 305
Query: 314 TIVIADPIYSPQHPIWLVDMIVKFLSENGKVYLQIPIRPKYESERELLWKLLEENDLNVI 373
I+ AD +Y L + ++ L + K + + + ES E L+ L ++
Sbjct: 306 LIIAADVVYDTALLASLCN-VLNLLLRHSKAAI-VACTRRNESSIECFEHHLKMAKLEIV 363
Query: 374 AEVQ-EKGKDDWGDVDYLYKEI 394
+ + E G G+ Y Y I
Sbjct: 364 EKFEYENGAYTIGENSYHYASI 385
>Hs20562158 [R] KOG2497 Predicted methyltransferase
Length = 330
Score = 51.6 bits (122), Expect = 2e-06
Identities = 56/228 (24%), Positives = 99/228 (42%), Gaps = 24/228 (10%)
Query: 123 NNLLRSYSDLCTKIPALYHNRSSESELLNYFTTILSKYETSSNELANQILQQASSRISER 182
+ LLR K P + S + + ++ K+E E +++ + + + +
Sbjct: 44 SELLRDILHKTVKHPVCVKHPPSVKYARCFLSELIKKHEAVHTEPLDELYEALAETLMAK 103
Query: 183 CGRTAQPSMTRTFKL-EGLDTSIKLYEPALTSDNLGLKTWGSSLMLSQKIIGIPA---GK 238
R++ L G ++ ++ GL TW ++L L++ I PA +
Sbjct: 104 ESTQGH----RSYLLPSGGSVTLSESTAIISYGTTGLVTWDAALYLAEWAIENPAVFTNR 159
Query: 239 RVLELGSGTGLVGISYALTHSINGDSVIFLTDLPEILPNLQYNVRLNNLN---DVTADV- 294
VLELGSG GL G+ A+ + IF +L L+ NV LN L+ D+TA +
Sbjct: 160 TVLELGSGAGLTGL--AICKMCRPRAYIFSDCHSRVLEQLRGNVLLNGLSLEADITAKLD 217
Query: 295 --------LDWTDPLSFTEKYGNEPFDTIVIADPIYSPQHPIWLVDMI 334
LDW D + + +P D ++ AD +Y P+ + LV ++
Sbjct: 218 SPRVTVAQLDW-DVATVHQLSAFQP-DVVIAADVLYCPEAIMSLVGVL 263
>At5g44170 [A] KOG2793 Putative N2N2-dimethylguanosine tRNA methyltransferase
Length = 234
Score = 51.2 bits (121), Expect = 3e-06
Identities = 43/161 (26%), Positives = 83/161 (50%), Gaps = 21/161 (13%)
Query: 238 KRVLELGSGTGLVGISYALTHSINGDSVIFLTDLPEILPNLQYNVRLNNL---NDVTADV 294
+R +ELG+G G+ G+++ L G + I LTD+ ++P L++N++ N + +
Sbjct: 71 RRGIELGTGCGVAGMAFYLL----GLTEIVLTDIAPVMPALKHNLKRNKTALGKSLKTSI 126
Query: 295 LDWT--DPLSFTEKYGNEPFDTIVIADPIYSPQHPIWLVDMIVKFLSENGKVYLQIPIRP 352
+ W D +S + PFD ++ AD +Y + LV + ++++G V L IR
Sbjct: 127 VYWNNRDQISALK----PPFDLVIAADVVYIEESVGQLVTAMELLVADDGAVLLGYQIRS 182
Query: 353 KYESERELLWKLLEENDLNVIAEVQEKGKDDWGDVDYLYKE 393
E+++ L W+L + ++ ++ EK + DY Y+E
Sbjct: 183 P-EADK-LFWELCD-----IVFKI-EKVPHEHLHSDYAYEE 215
>Hs17475528 [A] KOG2793 Putative N2N2-dimethylguanosine tRNA methyltransferase
Length = 257
Score = 50.8 bits (120), Expect = 3e-06
Identities = 35/104 (33%), Positives = 56/104 (53%), Gaps = 12/104 (11%)
Query: 238 KRVLELGSGTGLVGISYALTHSINGDSVIFLTDLPEILPNLQYNV----RLNNLNDVTAD 293
K V+E+G+GTGLV I +L + + TDLPE+L NLQYN+ ++ + +
Sbjct: 104 KNVIEIGAGTGLVSIVASLL-----GAHVTATDLPELLGNLQYNISRNTKMKSKHLPQVK 158
Query: 294 VLDWTDPLSFTEKYGNEPFDTIVIADPIYSPQHPIWLVDMIVKF 337
L W L + FD I+ AD +Y+ HP +L ++++ F
Sbjct: 159 ELSWGVALDTNFPRSSNNFDYILAADVVYA--HP-FLEELLITF 199
>At5g49560 [A] KOG2793 Putative N2N2-dimethylguanosine tRNA methyltransferase
Length = 274
Score = 50.8 bits (120), Expect = 3e-06
Identities = 26/51 (50%), Positives = 34/51 (65%), Gaps = 4/51 (7%)
Query: 235 PAGKRVLELGSGTGLVGISYALTHSINGDSVIFLTDLPEILPNLQYNVRLN 285
P+ +LELGSGTGLVGI+ A+T S N + +TDLP +L NL +N N
Sbjct: 104 PSPLNILELGSGTGLVGIAAAITLSAN----VTVTDLPHVLDNLNFNAEAN 150
>SPAC23C4.06c [A] KOG2793 Putative N2N2-dimethylguanosine tRNA methyltransferase
Length = 327
Score = 50.1 bits (118), Expect = 6e-06
Identities = 41/161 (25%), Positives = 80/161 (49%), Gaps = 19/161 (11%)
Query: 221 WGSSLMLSQKIIGIP--------AGKRVLELGSGTGLVGISYALTHSINGDSVIFLTDLP 272
W + ++ S+KI+ VLELGSG G+VGIS A + +++ +TD
Sbjct: 159 WDAGVVFSKKILSDDWHYSFSNRKDINVLELGSGCGIVGISIASKYP---RALVSMTDTE 215
Query: 273 EILPNLQYNVRLNN---LNDVTADVLDWTDPLSFTEKYGNEPFDTIVIADPIYSPQHPIW 329
+ + ++ NV N N++T+D+L W + + +D IV++D +Y+
Sbjct: 216 DAIEFMEKNVEKNKSAMSNNITSDILVWGHDI---PRKFRRHWDYIVMSDVMYNESSFSD 272
Query: 330 LVDMIVKFLSENGKVYLQIPIRPKYESERELLWKLLEENDL 370
L + + + +N K+Y I + ++++E+ + +L DL
Sbjct: 273 LEASLQELMDKNTKLY--IAYKKRHDNEKTFMSNILGWLDL 311
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.315 0.134 0.392
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 24,749,701
Number of Sequences: 60738
Number of extensions: 1119551
Number of successful extensions: 2489
Number of sequences better than 1.0e-05: 11
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 2472
Number of HSP's gapped (non-prelim): 11
length of query: 400
length of database: 30,389,216
effective HSP length: 109
effective length of query: 291
effective length of database: 23,768,774
effective search space: 6916713234
effective search space used: 6916713234
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)