ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV5611 good O KOG0404 Posttranslational modification, protein turnover, chaperones Thioredoxin reductase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV5611 1928736 1927690 -349
(349 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDR353w [O] KOG0404 Thioredoxin reductase 572 e-163
YHR106w [O] KOG0404 Thioredoxin reductase 538 e-153
At4g35460 [O] KOG0404 Thioredoxin reductase 398 e-111
At2g17420 [O] KOG0404 Thioredoxin reductase 397 e-110
At2g41680_1 [O] KOG0404 Thioredoxin reductase 318 7e-87
ECU02g0940 [O] KOG0404 Thioredoxin reductase 233 4e-61
SPAPB1E7.07 [E] KOG0399 Glutamate synthase 51 2e-06
Hs21389617 [R] KOG1336 Monodehydroascorbate/ferredoxin reductase 51 3e-06
>YDR353w [O] KOG0404 Thioredoxin reductase
Length = 319
Score = 572 bits (1473), Expect = e-163
Identities = 281/319 (88%), Positives = 301/319 (94%)
Query: 31 MVHHKVTIIGSGPAAHTAAIYLARAEIKPTLYEGFMANGIAAGGQLTTTTEIENFPGFPD 90
MVH+KVTIIGSGPAAHTAAIYLARAEIKP LYEG MANGIAAGGQLTTTTEIENFPGFPD
Sbjct: 1 MVHNKVTIIGSGPAAHTAAIYLARAEIKPILYEGMMANGIAAGGQLTTTTEIENFPGFPD 60
Query: 91 GLTGSELMDRMKAQSIKFGTDVITETVSKVDLSSRPFKFWTEFNEDQEPETTDAIILSTG 150
GLTGSELMDRM+ QS KFGT++ITETVSKVDLSS+PFK WTEFNED EP TTDAIIL+TG
Sbjct: 61 GLTGSELMDRMREQSTKFGTEIITETVSKVDLSSKPFKLWTEFNEDAEPVTTDAIILATG 120
Query: 151 ASAKRLHLPGEETYWQQGISACAVCDGAVPIFRNKPLAVIGGGDSACEEAQFLTKYGSKV 210
ASAKR+HLPGEETYWQ+GISACAVCDGAVPIFRNKPLAVIGGGDSACEEAQFLTKYGSKV
Sbjct: 121 ASAKRMHLPGEETYWQKGISACAVCDGAVPIFRNKPLAVIGGGDSACEEAQFLTKYGSKV 180
Query: 211 YMLVRKDHLRASQIMQRRAEQNEKIEILYNHVTLEAKGDGKYLNALKVKNVKTNEEYDLP 270
+MLVRKDHLRAS IMQ+RAE+NEKIEILYN V LEAKGDGK LNAL++KN K NEE DLP
Sbjct: 181 FMLVRKDHLRASTIMQKRAEKNEKIEILYNTVALEAKGDGKLLNALRIKNTKKNEETDLP 240
Query: 271 VNGLFYAIGHTPATNIVAGQVDLDEAGYVKTVPGSTLTNVPGVFAAGDVQDARYRQAITS 330
V+GLFYAIGHTPAT IVAGQVD DEAGY+KTVPGS+LT+VPG FAAGDVQD++YRQAITS
Sbjct: 241 VSGLFYAIGHTPATKIVAGQVDTDEAGYIKTVPGSSLTSVPGFFAAGDVQDSKYRQAITS 300
Query: 331 AGSGCMAALDAEKYITELE 349
AGSGCMAALDAEKY+T LE
Sbjct: 301 AGSGCMAALDAEKYLTSLE 319
>YHR106w [O] KOG0404 Thioredoxin reductase
Length = 342
Score = 538 bits (1385), Expect = e-153
Identities = 264/338 (78%), Positives = 301/338 (88%), Gaps = 1/338 (0%)
Query: 12 LSSLRGFFRNINESNIFYRMVHHKVTIIGSGPAAHTAAIYLARAEIKPTLYEGFMANGIA 71
+S R F I++S + RM+HHKVTIIGSGPAAHTAAIYLARAE+KPTLYEG MANGIA
Sbjct: 6 VSPFRTNFVGISKS-VLSRMIHHKVTIIGSGPAAHTAAIYLARAEMKPTLYEGMMANGIA 64
Query: 72 AGGQLTTTTEIENFPGFPDGLTGSELMDRMKAQSIKFGTDVITETVSKVDLSSRPFKFWT 131
AGGQLTTTT+IENFPGFP+ L+GSELM+RM+ QS KFGT++ITETVSKVDLSS+PF+ WT
Sbjct: 65 AGGQLTTTTDIENFPGFPESLSGSELMERMRKQSAKFGTNIITETVSKVDLSSKPFRLWT 124
Query: 132 EFNEDQEPETTDAIILSTGASAKRLHLPGEETYWQQGISACAVCDGAVPIFRNKPLAVIG 191
EFNED EP TTDAIIL+TGASAKR+HLPGEETYWQQGISACAVCDGAVPIFRNKPLAVIG
Sbjct: 125 EFNEDAEPVTTDAIILATGASAKRMHLPGEETYWQQGISACAVCDGAVPIFRNKPLAVIG 184
Query: 192 GGDSACEEAQFLTKYGSKVYMLVRKDHLRASQIMQRRAEQNEKIEILYNHVTLEAKGDGK 251
GGDSACEEA+FLTKY SKVY+LVRKDH RAS IMQRR E+N I +L+N V LEAKGDGK
Sbjct: 185 GGDSACEEAEFLTKYASKVYILVRKDHFRASVIMQRRIEKNPNIIVLFNTVALEAKGDGK 244
Query: 252 YLNALKVKNVKTNEEYDLPVNGLFYAIGHTPATNIVAGQVDLDEAGYVKTVPGSTLTNVP 311
LN L++KN K+N E DL VNGLFYAIGH+PAT+IV GQVD +E GY+KTVPGS+LT+VP
Sbjct: 245 LLNMLRIKNTKSNVENDLEVNGLFYAIGHSPATDIVKGQVDEEETGYIKTVPGSSLTSVP 304
Query: 312 GVFAAGDVQDARYRQAITSAGSGCMAALDAEKYITELE 349
G FAAGDVQD+RYRQA+TSAGSGC+AALDAE+Y++ E
Sbjct: 305 GFFAAGDVQDSRYRQAVTSAGSGCIAALDAERYLSAQE 342
>At4g35460 [O] KOG0404 Thioredoxin reductase
Length = 333
Score = 398 bits (1023), Expect = e-111
Identities = 203/320 (63%), Positives = 248/320 (77%), Gaps = 10/320 (3%)
Query: 35 KVTIIGSGPAAHTAAIYLARAEIKPTLYEGFMANGIAAGGQLTTTTEIENFPGFPDGLTG 94
++ I+GSGPAAHTAAIY ARAE+KP L+EG+MAN IA GGQLTTTT++ENFPGFP+G+ G
Sbjct: 10 RLCIVGSGPAAHTAAIYAARAELKPLLFEGWMANDIAPGGQLTTTTDVENFPGFPEGILG 69
Query: 95 SELMDRMKAQSIKFGTDVITETVSKVDLSSRPFKFWTEFNEDQEPETTDAIILSTGASAK 154
EL D+ + QS +FGT + TETV+KVD SS+PFK +T D + DA+IL+TGA AK
Sbjct: 70 VELTDKFRKQSERFGTTIFTETVTKVDFSSKPFKLFT----DSKAILADAVILATGAVAK 125
Query: 155 RLHLPGEET----YWQQGISACAVCDGAVPIFRNKPLAVIGGGDSACEEAQFLTKYGSKV 210
RL G +W +GISACAVCDGA PIFRNKPLAVIGGGDSA EEA FLTKYGSKV
Sbjct: 126 RLSFVGSGEASGGFWNRGISACAVCDGAAPIFRNKPLAVIGGGDSAMEEANFLTKYGSKV 185
Query: 211 YMLVRKDHLRASQIMQRRAEQNEKIEILYNHVTLEAKGDGK--YLNALKVKNVKTNEEYD 268
Y++ R+D RAS+IMQ+RA N KI++++N +EA GDG+ L LKVKNV T + D
Sbjct: 186 YIIHRRDAFRASKIMQQRALSNPKIDVIWNSSVVEAYGDGERDVLGGLKVKNVVTGDVSD 245
Query: 269 LPVNGLFYAIGHTPATNIVAGQVDLDEAGYVKTVPGSTLTNVPGVFAAGDVQDARYRQAI 328
L V+GLF+AIGH PAT + G V+LD GYV T PG+T T+VPGVFAAGDVQD +YRQAI
Sbjct: 246 LKVSGLFFAIGHEPATKFLDGGVELDSDGYVVTKPGTTQTSVPGVFAAGDVQDKKYRQAI 305
Query: 329 TSAGSGCMAALDAEKYITEL 348
T+AG+GCMAALDAE Y+ E+
Sbjct: 306 TAAGTGCMAALDAEHYLQEI 325
>At2g17420 [O] KOG0404 Thioredoxin reductase
Length = 383
Score = 397 bits (1020), Expect = e-110
Identities = 200/320 (62%), Positives = 248/320 (77%), Gaps = 10/320 (3%)
Query: 35 KVTIIGSGPAAHTAAIYLARAEIKPTLYEGFMANGIAAGGQLTTTTEIENFPGFPDGLTG 94
KV I+GSGPAAHTAAIY +RAE+KP L+EG+MAN IA GGQLTTTT++ENFPGFP+G+ G
Sbjct: 60 KVCIVGSGPAAHTAAIYASRAELKPLLFEGWMANDIAPGGQLTTTTDVENFPGFPEGILG 119
Query: 95 SELMDRMKAQSIKFGTDVITETVSKVDLSSRPFKFWTEFNEDQEPETTDAIILSTGASAK 154
+++++ + QS +FGT + TETV+KVD SS+PFK +T D D++I+STGA AK
Sbjct: 120 IDIVEKFRKQSERFGTTIFTETVNKVDFSSKPFKLFT----DSRTVLADSVIISTGAVAK 175
Query: 155 RLHLPGEET----YWQQGISACAVCDGAVPIFRNKPLAVIGGGDSACEEAQFLTKYGSKV 210
RL G +W +GISACAVCDGA PIFRNKPL VIGGGDSA EEA FLTKYGSKV
Sbjct: 176 RLSFTGSGEGNGGFWNRGISACAVCDGAAPIFRNKPLVVIGGGDSAMEEANFLTKYGSKV 235
Query: 211 YMLVRKDHLRASQIMQRRAEQNEKIEILYNHVTLEAKGD--GKYLNALKVKNVKTNEEYD 268
Y++ R+D RAS+IMQ+RA N KIE+++N +EA GD G+ L LKVKNV T + D
Sbjct: 236 YIIHRRDTFRASKIMQQRALSNPKIEVIWNSAVVEAYGDENGRVLGGLKVKNVVTGDVSD 295
Query: 269 LPVNGLFYAIGHTPATNIVAGQVDLDEAGYVKTVPGSTLTNVPGVFAAGDVQDARYRQAI 328
L V+GLF+AIGH PAT + GQ++LDE GYV T PG+T T+V GVFAAGDVQD +YRQAI
Sbjct: 296 LKVSGLFFAIGHEPATKFLDGQLELDEDGYVVTKPGTTKTSVVGVFAAGDVQDKKYRQAI 355
Query: 329 TSAGSGCMAALDAEKYITEL 348
T+AG+GCMAALDAE Y+ E+
Sbjct: 356 TAAGTGCMAALDAEHYLQEI 375
>At2g41680_1 [O] KOG0404 Thioredoxin reductase
Length = 396
Score = 318 bits (815), Expect = 7e-87
Identities = 157/312 (50%), Positives = 222/312 (70%), Gaps = 6/312 (1%)
Query: 36 VTIIGSGPAAHTAAIYLARAEIKPTLYEGFMANGIAAGGQLTTTTEIENFPGFPDGLTGS 95
V IIGSGPA +TAAIY ARA +KP ++EG+ G+ GGQL TTTE+ENFPGFPDG+TG
Sbjct: 86 VVIIGSGPAGYTAAIYAARANLKPVVFEGYQMGGVP-GGQLMTTTEVENFPGFPDGITGP 144
Query: 96 ELMDRMKAQSIKFGTDVITETVSKVDLSSRPFKFWTEFNEDQEPETTDAIILSTGASAKR 155
+LM++M+ Q+ ++G ++ E V + +++ PF T + +II +TGA+A+R
Sbjct: 145 DLMEKMRKQAERWGAELYPEDVESLSVTTAPFTVQTS----ERKVKCHSIIYATGATARR 200
Query: 156 LHLPGEETYWQQGISACAVCDGAVPIFRNKPLAVIGGGDSACEEAQFLTKYGSKVYMLVR 215
L LP EE +W +GISACA+CDGA P+F+ + LAV+GGGD+A EEA +LTKY V++LVR
Sbjct: 201 LRLPREEEFWSRGISACAICDGASPLFKGQVLAVVGGGDTATEEALYLTKYARHVHLLVR 260
Query: 216 KDHLRASQIMQRRAEQNEKIEILYNHVTLEAKGDGK-YLNALKVKNVKTNEEYDLPVNGL 274
+D LRAS+ MQ R N I + YN T++ + K ++ + ++ + T EE +L GL
Sbjct: 261 RDQLRASKAMQDRVINNPNITVHYNTETVDVLSNTKGQMSGILLRRLDTGEETELEAKGL 320
Query: 275 FYAIGHTPATNIVAGQVDLDEAGYVKTVPGSTLTNVPGVFAAGDVQDARYRQAITSAGSG 334
FY IGH+P + ++ GQV+LD +GYV G++ T+V GVFAAGDVQD +RQA+T+AGSG
Sbjct: 321 FYGIGHSPNSQLLEGQVELDSSGYVLVREGTSNTSVEGVFAAGDVQDHEWRQAVTAAGSG 380
Query: 335 CMAALDAEKYIT 346
C+AAL AE+Y+T
Sbjct: 381 CIAALSAERYLT 392
>ECU02g0940 [O] KOG0404 Thioredoxin reductase
Length = 309
Score = 233 bits (593), Expect = 4e-61
Identities = 130/313 (41%), Positives = 193/313 (61%), Gaps = 13/313 (4%)
Query: 36 VTIIGSGPAAHTAAIYLARAEIKPTLYEGFMANGIAAGGQLTTTTEIENFPGFPDGLTGS 95
+ IIGSGPAA+ AA+Y E P L+EG GGQLTTTT ++N+PGFPDG+ G
Sbjct: 4 IVIIGSGPAAYNAALYAMDKE--PLLFEGGYIGNNGPGGQLTTTTSVDNYPGFPDGVEGP 61
Query: 96 ELMDRMKAQSIKFGTDVITETVSKVDLSSRPFKFWTEFNEDQEPETTDAIILSTGASAKR 155
L D MK ++ G V+ ETVS + F +E E A+I++TGASA+R
Sbjct: 62 VLTDLMKDHAVSRGLRVVKETVSDLRKEGECFVVVSEKGE----RRARAVIVATGASARR 117
Query: 156 LHLP--GEETYWQQGISACAVCDGAVPIFRNKPLAVIGGGDSACEEAQFLTKYGSKVYML 213
L +P G+ +WQ+G+S+CAVCDG + NK VIGGGD+A EEA +L+ KVY++
Sbjct: 118 LFVPGTGDGEFWQRGVSSCAVCDGFA--YTNKITCVIGGGDAAMEEALYLSGIAKKVYII 175
Query: 214 VRKDHLRASQIMQRRAEQNEKIEILYNHVTLEAKGDGKYLNALKVKNVKTNEEYDLPVNG 273
R++ RA M +A + IE++ +V A G K + + ++NV+T E +P++G
Sbjct: 176 HRRNEFRARNDMVEKARETVNIEMVTPYVLESALGTSK-VERITIRNVETGEMKTIPMDG 234
Query: 274 LFYAIGHTPATNIVAG-QVDLDEAGYVKTVPGSTLTNVPGVFAAGDVQDARYRQAITSAG 332
+F+ IGH P T+ + +V++D GY+ V TN+PG+FAAGDV D +YRQA+T+A
Sbjct: 235 VFFGIGHDPNTSFLKNTKVEMDLNGYI-VVRDEVYTNIPGLFAAGDVCDKKYRQAVTAAA 293
Query: 333 SGCMAALDAEKYI 345
SG ++ + A +++
Sbjct: 294 SGAISGMKAVEFL 306
>SPAPB1E7.07 [E] KOG0399 Glutamate synthase
Length = 2111
Score = 51.2 bits (121), Expect = 2e-06
Identities = 74/332 (22%), Positives = 131/332 (39%), Gaps = 70/332 (21%)
Query: 35 KVTIIGSGPAAHTAAIYLARAEIKPTLYEGFMANGIAAGGQLTTTTEIENFPGFPDGLTG 94
+V IIGSGPA AA L RA +YE GG L G P+
Sbjct: 1757 RVAIIGSGPAGLAAADQLNRAGHHVVIYE----RADRPGGLLQY--------GIPNMKLD 1804
Query: 95 SELMDRMKAQSIKFGTDVITETVSKVDLSSRPFKFWTEFNEDQEPETTDAIILSTGASAK 154
++++R I G +V+T V++ + + D+ + DA++L++G++
Sbjct: 1805 KKVVERRIQLMIDEGIEVLT----NVEVGKN-----GDVSLDELHKVYDAVVLASGSTVP 1855
Query: 155 R-LHLPGEETYW-----------QQGISACAVCDGAVPIFRNKPLAVIGGGDSACEEAQF 202
R L +P ++ + + + DG + K + VIGGGD+ +
Sbjct: 1856 RDLPIPNRDSKGIHFAMEFLHKNTKSLLDSELKDGNYISAKGKDVIVIGGGDTGNDCLGT 1915
Query: 203 LTKYGSK----------------------VYMLVRKDHLRASQIMQRRAEQNEKIEILYN 240
++G+K Y V + +++ + + IL
Sbjct: 1916 SVRHGAKSVRNLELLPIPPRERAFDNPWPQYPRVFRVDYGHAEVQAHYGQDFREYSILTK 1975
Query: 241 HVTLEAKGDGKYLNALKV------------KNVKTNEEYDLPVNGLFYAIGHT-PATNIV 287
+ G+ K +N +++ K ++ +EE+ P + + A+G P
Sbjct: 1976 SFEKDEDGNVKGINTVRIEWTKNSKGRWIMKEIRNSEEF-FPADLVILALGFLGPEEQAT 2034
Query: 288 AG-QVDLDEAGYVKTVPGSTLTNVPGVFAAGD 318
AG VD D + T S T+VPG++AAGD
Sbjct: 2035 AGMNVDRDARSNISTPTKSYETSVPGIYAAGD 2066
>Hs21389617 [R] KOG1336 Monodehydroascorbate/ferredoxin reductase
Length = 605
Score = 50.8 bits (120), Expect = 3e-06
Identities = 57/219 (26%), Positives = 96/219 (43%), Gaps = 19/219 (8%)
Query: 108 FGTDVITET-VSKVDLSSRPFKFWTEFNEDQEPETTDAIILSTGASAKRLHLPGEETYWQ 166
+G +V+TE V VD+ ++ F F + ++L+ G+S K L G+E
Sbjct: 260 YGIEVLTEAQVVTVDVRTKKVVFKDGFKLEYSK-----LLLAPGSSPKTLSCKGKEVENV 314
Query: 167 QGISACAVCDGAVPIFRNKPLAVIGGGDSACEEAQFLTKYGSKVYMLVRKDHLRASQIMQ 226
I + V + R + + V+G G E A +LT+ V ++ ++ + +
Sbjct: 315 FTIRTPEDANRVVRLARGRNVVVVGAGFLGMEVAAYLTEKAHSVSVVELEETPFRRFLGE 374
Query: 227 R------RAEQNEKIEILYNHVTLEAKGDGKYLNALKVKNVKTNEEYDLPVNGLFYAIGH 280
R + +N +++ E +G L + +K+ K D+ V G IG
Sbjct: 375 RVGRALMKMFENNRVKFYMQTEVSELRGQEGKLKEVVLKSSKVVRA-DVCVVG----IGA 429
Query: 281 TPATNIVAGQ-VDLDEAGYVKTVPGSTLTNVPGVFAAGD 318
PAT + + LD G++ V TNVPGVFAAGD
Sbjct: 430 VPATGFLRQSGIGLDSRGFIP-VNKMMQTNVPGVFAAGD 467
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.134 0.385
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 20,429,735
Number of Sequences: 60738
Number of extensions: 857713
Number of successful extensions: 2025
Number of sequences better than 1.0e-05: 8
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 2001
Number of HSP's gapped (non-prelim): 9
length of query: 349
length of database: 30,389,216
effective HSP length: 107
effective length of query: 242
effective length of database: 23,890,250
effective search space: 5781440500
effective search space used: 5781440500
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)