ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV5670 good J KOG2713 Translation, ribosomal structure and biogenesis Mitochondrial tryptophanyl-tRNA synthetase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV5670 1947496 1948629 378
(378 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDR268w [J] KOG2713 Mitochondrial tryptophanyl-tRNA synthetase 476 e-134
SPAC3G9.13c [J] KOG2713 Mitochondrial tryptophanyl-tRNA synthetase 268 7e-72
Hs7710154 [J] KOG2713 Mitochondrial tryptophanyl-tRNA synthetase 268 1e-71
7293931 [J] KOG2713 Mitochondrial tryptophanyl-tRNA synthetase 253 2e-67
CE29032_2 [J] KOG2713 Mitochondrial tryptophanyl-tRNA synthetase 215 1e-55
At2g25840 [J] KOG2713 Mitochondrial tryptophanyl-tRNA synthetase 193 4e-49
>YDR268w [J] KOG2713 Mitochondrial tryptophanyl-tRNA synthetase
Length = 379
Score = 476 bits (1225), Expect = e-134
Identities = 230/371 (61%), Positives = 291/371 (77%), Gaps = 6/371 (1%)
Query: 13 RILFKRFNSSVKTTGFKLRESDLPKNATIFSLIQPTGKFHLGNYLGAVRVWKDITDLKDD 72
+++ KR+ S+V+ FKL L NAT+FS+IQPTG FHLGNYLGA RVW D+ +LK
Sbjct: 9 KLISKRWISTVQRADFKLNSEALHSNATVFSMIQPTGCFHLGNYLGATRVWTDLCELKQP 68
Query: 73 GTTLLFGTADLHAITVPKPNAKEFRNYRIEAIASILSIGIDPEKAIVFHQSRVSQHTELH 132
G L+FG ADLHAITVPKP+ + FR +R EA+ASIL++G+DPEKA V +QS + QH+ELH
Sbjct: 69 GQELIFGVADLHAITVPKPDGEMFRKFRHEAVASILAVGVDPEKASVIYQSAIPQHSELH 128
Query: 133 WLLSSLASMGSLNRMTQWKSKSNINDQSD------DAALGAIKLGLFSYPVLQAADILLY 186
WLLS+LASMG LNRMTQWKSKSNI ++ D+ +G ++LGLFSYPVLQAADILLY
Sbjct: 129 WLLSTLASMGLLNRMTQWKSKSNIKQSTNGDYLVNDSDVGKVRLGLFSYPVLQAADILLY 188
Query: 187 KSTHVPVGDDQSQHLELTRQLSNTFNKFYKTKYFPMPTTILAPTKKILSLLNPEKKMSKS 246
KSTHVPVGDDQSQHLELTR L+ FNK YK +FP P T+LA TKK+LSL PEKKMSKS
Sbjct: 189 KSTHVPVGDDQSQHLELTRHLAEKFNKMYKKNFFPKPVTMLAQTKKVLSLSTPEKKMSKS 248
Query: 247 DTNQNSVIYITDEPEIIAKKIKRAVTDSISDSFYFDPVKRPGISNLINTLSGVQRVSIED 306
D N +SVI++ DEP+ I KKI++A+TDSISD FY+DPV+RPG+SNLIN +SG+QR SIED
Sbjct: 249 DPNHDSVIFLNDEPKAIQKKIRKALTDSISDRFYYDPVERPGVSNLINIVSGIQRKSIED 308
Query: 307 VEKDIAHIKDHKDFKQHVTDVLVEELAPSREQFHRYMKNPDYLHKVSEDGASKAREIAAK 366
V +D++ +++DFK +V++V++EEL R +F +Y+ P YLH V E G KARE AAK
Sbjct: 309 VVEDVSRFNNYRDFKDYVSEVIIEELKGPRTEFEKYINEPTYLHSVVESGMRKAREKAAK 368
Query: 367 NITEIKRIMGF 377
N+ +I +IMGF
Sbjct: 369 NLADIHKIMGF 379
>SPAC3G9.13c [J] KOG2713 Mitochondrial tryptophanyl-tRNA synthetase
Length = 361
Score = 268 bits (686), Expect = 7e-72
Identities = 150/353 (42%), Positives = 220/353 (61%), Gaps = 12/353 (3%)
Query: 33 SDLPKNATIFSLIQPTGKFHLGNYLGAVRVWKDITD--LKDDGTTLLFGTADLHAITVPK 90
+ L ++ + S IQPTG H+GNYLG+++ W + + + + F ADLHA+TVP+
Sbjct: 8 TSLLPHSRVVSGIQPTGIPHIGNYLGSLKQWVQLQEEAARTPFSKCFFFVADLHALTVPQ 67
Query: 91 PNAKEFRNYRIEAIASILSIGIDPEKAIVFHQSRVSQHTELHWLLSSLASMGSLNRMTQW 150
K FR R++ +A++L+IGI+P+K+ +F QS V+QH+EL WLL+ SMG LNRMTQW
Sbjct: 68 DPLK-FRQARLDMLAALLAIGINPQKSTLFFQSDVAQHSELAWLLACSTSMGQLNRMTQW 126
Query: 151 KSKSNINDQSDDAALGA-------IKLGLFSYPVLQAADILLYKSTHVPVGDDQSQHLEL 203
KSK +++D D + L A LGLFSYPVLQAADILLY +TH+PVG DQSQH+EL
Sbjct: 127 KSKLHLHDHDDLSFLDASATSSTRFNLGLFSYPVLQAADILLYGATHIPVGKDQSQHVEL 186
Query: 204 TRQLSNTFNKFYKTKYFPMPTTILAPTKKILSLLNPEKKMSKSDTNQNSVIYITDEPEII 263
TR ++ +FN YK K +P IL + I++L PEKKMSKSD N + I ++D I
Sbjct: 187 TRSIARSFNSSYKEKILTVPDIILNSSSSIMALCQPEKKMSKSDINSKNYILLSDSTGEI 246
Query: 264 AKKIKRAVTDSISDSFYFDPVKRPGISNLINTLSGVQRVSIEDVEKDIAHIKDHKDFKQH 323
KKI RA TD+I Y D RPGI+NLIN + + + D+ + A + +FK+
Sbjct: 247 RKKISRAQTDNIKGITYGDS-NRPGINNLINIFAAISDSTPSDIAQANASC-SNAEFKEK 304
Query: 324 VTDVLVEELAPSREQFHRYMKNPDYLHKVSEDGASKAREIAAKNITEIKRIMG 376
V+ ++ L P F+ + +N + L +++ GA +A A+ + ++K + G
Sbjct: 305 VSSAIIRCLQPISTSFNEWRQNRELLRDIAKKGAEEAVAEASSCMHKLKTLTG 357
>Hs7710154 [J] KOG2713 Mitochondrial tryptophanyl-tRNA synthetase
Length = 360
Score = 268 bits (684), Expect = 1e-71
Identities = 147/338 (43%), Positives = 220/338 (64%), Gaps = 14/338 (4%)
Query: 41 IFSLIQPTGKFHLGNYLGAVRVWKDITDLKDDGTTLLFGTADLHAITVPKPNAKEFRNYR 100
+FS IQPTG HLGNYLGA+ W L+D+ ++L+ DLH+ITVP+ A R
Sbjct: 37 VFSGIQPTGILHLGNYLGAIESW---VRLQDEYDSVLYSIVDLHSITVPQDPAV-LRQSI 92
Query: 101 IEAIASILSIGIDPEKAIVFHQSRVSQHTELHWLLSSLASMGSLNRMTQWKSKSNINDQS 160
++ A +L+ GI+PEK+I+F QS+VS+HT+L W+LS + + L + QWK+K+ Q
Sbjct: 93 LDMTAVLLACGINPEKSILFQQSQVSEHTQLSWILSCMVRLPRLQHLHQWKAKTT--KQK 150
Query: 161 DDAALGAIKLGLFSYPVLQAADILLYKSTHVPVGDDQSQHLELTRQLSNTFNKFYKTKYF 220
D +G L +YPVLQAADILLYKSTHVPVG+DQ QH+EL + L+ FNK Y ++F
Sbjct: 151 HDGTVG-----LLTYPVLQAADILLYKSTHVPVGEDQVQHMELVQDLAQGFNKKY-GEFF 204
Query: 221 PMPTTILAPTKKILSLLNPEKKMSKSDTNQNSVIYITDEPEIIAKKIKRAVTDSISDSFY 280
P+P +IL KK+ SL +P KMSKSD ++ + + ITD PE I +K ++AVTD S+ Y
Sbjct: 205 PVPESILTSMKKVKSLRDPSAKMSKSDPDKLATVRITDSPEEIVQKFRKAVTDFTSEVTY 264
Query: 281 FDPVKRPGISNLINTLSGVQRVSIEDVEKDIAHIKDHKDFKQHVTDVLVEELAPSREQFH 340
DP R G+SN++ + V +S+E+V + A + + +K V D ++E+ AP + +
Sbjct: 265 -DPAGRAGVSNIVAVHAAVTGLSVEEVVRRSAGMNTAR-YKLAVADAVIEKFAPIKREIE 322
Query: 341 RYMKNPDYLHKVSEDGASKAREIAAKNITEIKRIMGFL 378
+ + D+L KV + G++KA+E+A E+K+++GFL
Sbjct: 323 KLKLDKDHLEKVLQIGSAKAKELAYTVCQEVKKLVGFL 360
>7293931 [J] KOG2713 Mitochondrial tryptophanyl-tRNA synthetase
Length = 665
Score = 253 bits (647), Expect = 2e-67
Identities = 146/338 (43%), Positives = 210/338 (61%), Gaps = 17/338 (5%)
Query: 41 IFSLIQPTGKFHLGNYLGAVRVWKDITDLKDDGTTLLFGTADLHAITVPKPNAKEFRNYR 100
+FS IQPTG HLGNYLGAVR W + + +DD T + DLH+IT+P N R
Sbjct: 82 VFSGIQPTGSLHLGNYLGAVRKWVQLQNARDDVTVCI---VDLHSITMPH-NPPLLRENI 137
Query: 101 IEAIASILSIGIDPEKAIVFHQSRVSQHTELHWLLSSLASMGSLNRMTQWKSKSNINDQS 160
A++L+ GIDP K+ +F QS V++H E +W+LSSL +M L ++ Q++ KS +
Sbjct: 138 FTMAATLLACGIDPTKSTLFVQSAVAEHAEFNWILSSLTTMPRLAQLPQFREKSRL---- 193
Query: 161 DDAALGAIKLGLFSYPVLQAADILLYKSTHVPVGDDQSQHLELTRQLSNTFNKFYKTKYF 220
L + LGL+ YPVLQAADI+LYKSTHVPVG DQ QH++L + L+ +N Y + F
Sbjct: 194 ----LKDVPLGLYVYPVLQAADIMLYKSTHVPVGADQIQHIQLAQHLARIYNGRY-GETF 248
Query: 221 PMPTTIL--APTKKILSLLNPEKKMSKSDTNQNSVIYITDEPEIIAKKIKRAVTDSISDS 278
P+ T I+ ++LSL +P KKMSKS+ N + I + D P++I +KIK+AVTD SD
Sbjct: 249 PVCTAIIEDGDASRVLSLRDPSKKMSKSEANPKATINLCDSPDLITQKIKKAVTDFTSDI 308
Query: 279 FYFDPVKRPGISNLINTLSGVQRVSIEDVEKDIAHIKDHKDFKQHVTDVLVEELAPSREQ 338
Y +P KR G+SNL+N + V SI+ V + A + D +K V + +VE L P REQ
Sbjct: 309 TY-NPGKRAGVSNLVNIHAQVTGQSIKTVVNEAATL-DTAKYKDRVAEAVVEHLRPIREQ 366
Query: 339 FHRYMKNPDYLHKVSEDGASKAREIAAKNITEIKRIMG 376
H +M + + + E GA KAR+ A + + ++K+ +G
Sbjct: 367 IHHHMTKRNEMIYLLEVGAEKARQQARQTLNDVKQRLG 404
>CE29032_2 [J] KOG2713 Mitochondrial tryptophanyl-tRNA synthetase
Length = 331
Score = 215 bits (547), Expect = 1e-55
Identities = 126/338 (37%), Positives = 193/338 (56%), Gaps = 12/338 (3%)
Query: 42 FSLIQPTGKFHLGNYLGAVRVWKDITDLKDDGTTLLFGTADLHAITVPKPNAKEFRNYRI 101
F+ IQPTG HLGN+ G++ W ++ + D ++ D HAI++ A E R
Sbjct: 5 FTGIQPTGIPHLGNFFGSIEPWTELQNSVDKNILMMLSVVDQHAISLGPLPANELRQNTH 64
Query: 102 EAIASILSIGIDPEKAIVFHQSRVSQHTELHWLLSSLASMGSLNRMTQWKSKSNINDQSD 161
+ AS+++ G+DP + ++F QS V Q ++ W+L SL + L R+ Q+K K + D
Sbjct: 65 QMTASLIACGVDPNRTLLFRQSDVPQIAQISWILGSLQTTSKLARLPQYKEKKERFKKGD 124
Query: 162 DAALGAIKLGLFSYPVLQAADILLYKSTHVPVGDDQSQHLELTRQLSNTFNKFYKTKYFP 221
I +GL +YP+LQAAD+L +K+T VPVG+DQSQHL L L+ FNK Y+T+ FP
Sbjct: 125 ------IPVGLLTYPLLQAADVLTFKATTVPVGEDQSQHLNLLGGLAYAFNKTYETEIFP 178
Query: 222 MPTTILAPT-KKILSLLNPEKKMSKSDTNQNSVIYITDEPEIIAKKIKRAVTDSISDSFY 280
+P + + +I SL PEKKMSKS S I ITD I +K ++A +D+ Y
Sbjct: 179 IPKQLTRESHARIRSLREPEKKMSKSSGGPRSRIEITDSRSTIIEKCQKAQSDNAGKVTY 238
Query: 281 FDPVKRPGISNLINTLSGVQRVSIEDVEKDIAHIKDHKDFKQHVTDVLVEELAPSREQFH 340
D R +SNL++ S V + ++ D ++ D K ++ + + + LAP R++F
Sbjct: 239 -DKENRLAVSNLLDLYSAVTKTQTSEI--DFSNWTT-LDLKMNLAEAVDKRLAPIRQKFE 294
Query: 341 RYMKNPDYLHKVSEDGASKAREIAAKNITEIKRIMGFL 378
++N + KV + KAREIA KN+ EI+R +GFL
Sbjct: 295 E-LQNTGEVDKVLTENGEKAREIAEKNLEEIRRTIGFL 331
>At2g25840 [J] KOG2713 Mitochondrial tryptophanyl-tRNA synthetase
Length = 347
Score = 193 bits (490), Expect = 4e-49
Identities = 130/352 (36%), Positives = 188/352 (52%), Gaps = 30/352 (8%)
Query: 41 IFSLIQPTGKFHLGNYLGAVRVWKDITDLKDDGTTLLFGTADLHAITVPKPNAKEFRNYR 100
+ S +QPTG HLGNYLGA++ W + D + LF D HAIT+P + ++
Sbjct: 5 VVSGVQPTGSVHLGNYLGAIKNWVALQDTYET----LFIIVDHHAITLPY-DTRQLGKAT 59
Query: 101 IEAIASILSIGIDPEKAIVFHQSRVSQHTELHWLLSSLASMGSLNRMTQWKSKSNINDQS 160
+ A L+ GID KA VF QS V H EL WLL S +G L +M Q+K KS +
Sbjct: 60 TDTAALYLACGIDVSKASVFVQSHVPAHVELMWLLCSSTPIGWLQKMIQFKEKSR-KEGV 118
Query: 161 DDAALGAIKLGLFSYPVLQAADILLYKSTHVPVGDDQSQHLELTRQLSNTFNKFYKTK-- 218
++A++G LF+YP L ADILLY+S VPVG+DQ QH+EL R+++ N Y K
Sbjct: 119 ENASVG-----LFTYPDLMTADILLYQSDFVPVGEDQKQHIELAREIAQRVNHLYGGKKW 173
Query: 219 ---------YFPMPTTILAPT--KKILSLLNPEKKMSKSDTNQNSVIYITDEPEIIAKKI 267
Y + L P +++SL + KMSKS + S I + D ++I KI
Sbjct: 174 KKLGGYEILYVHLIPEPLIPQAGARVMSLTDGLSKMSKSAPSDQSRINLLDSKDLIVDKI 233
Query: 268 KRAVTDSISDSFYFDPVKRPGISNLINTLSGVQRVSIEDVEKDIAHIKDHK--DFKQHVT 325
KR TDS + FD +RP +NL LS Q VS + E+ + KD FK +
Sbjct: 234 KRCKTDSFA-GLEFDNAERPECNNL---LSIYQIVSGKKKEEVMEECKDMSWGTFKPLLA 289
Query: 326 DVLVEELAPSREQFHRYMKNPDYLHKVSEDGASKAREIAAKNITEIKRIMGF 377
D L+E L+P + ++ + P+YL K+ +GA +A E+ A + + + MG+
Sbjct: 290 DALIEHLSPIQARYQEIIAEPEYLDKILSEGADRAEELGAVTMRNMYQAMGY 341
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.133 0.373
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,943,458
Number of Sequences: 60738
Number of extensions: 936292
Number of successful extensions: 2789
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 2758
Number of HSP's gapped (non-prelim): 7
length of query: 378
length of database: 30,389,216
effective HSP length: 108
effective length of query: 270
effective length of database: 23,829,512
effective search space: 6433968240
effective search space used: 6433968240
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)