ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV5747 good E KOG2630 Amino acid transport and metabolism Enolase-phosphatase E-1
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV5747 1973522 1974184 221
(221 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YEL038w [E] KOG2630 Enolase-phosphatase E-1 237 1e-62
SPAC644.08 [E] KOG2630 Enolase-phosphatase E-1 137 9e-33
At5g53850_2 [E] KOG2630 Enolase-phosphatase E-1 124 1e-28
Hs10864017 [E] KOG2630 Enolase-phosphatase E-1 121 9e-28
CE06027 [E] KOG2630 Enolase-phosphatase E-1 120 2e-27
7296775 [E] KOG2630 Enolase-phosphatase E-1 101 9e-22
>YEL038w [E] KOG2630 Enolase-phosphatase E-1
Length = 241
Score = 237 bits (604), Expect = 1e-62
Identities = 128/225 (56%), Positives = 160/225 (70%), Gaps = 14/225 (6%)
Query: 6 YILDIEGTVCPISFVKDTLYPFFLKQVESLVK--TNDPTLQNLLAQFPVPQDASSLHEHI 63
Y+LDIEGTVCPISFVK+TL+P+F +V LV+ T D + N+L+QF + + L HI
Sbjct: 22 YLLDIEGTVCPISFVKETLFPYFTNKVPQLVQQDTRDSPVSNILSQFHI-DNKEQLQAHI 80
Query: 64 ESLVNNDIKDSVLKQLQGYIWEQGYKSGEIKAPVYPDAIDFIQRHAPNVYIYSSGSVKAQ 123
LV D+KD +LKQLQGY+W GY+SG+IKAPVY DAIDFI+R V+IYSSGSVKAQ
Sbjct: 81 LELVAKDVKDPILKQLQGYVWAHGYESGQIKAPVYADAIDFIKR-KKRVFIYSSGSVKAQ 139
Query: 124 ILLFQHVE-------GDIDLTKSIAGYFDINTSGKKTEPQSYTNILKSIGVPPSSASDVV 176
LLF +V+ +DL I GYFDINTSGKKTE QSY NIL+ IG + AS+V+
Sbjct: 140 KLLFGYVQDPNAPAHDSLDLNSYIDGYFDINTSGKKTETQSYANILRDIG---AKASEVL 196
Query: 177 FISDNDKELDAALDVGISTILALRPGNNPVSNAEKYKALNNFSSI 221
F+SDN ELDAA VGI+T LA RPGN PV + +KY+ NF ++
Sbjct: 197 FLSDNPLELDAAAGVGIATGLASRPGNAPVPDGQKYQVYKNFETL 241
>SPAC644.08 [E] KOG2630 Enolase-phosphatase E-1
Length = 216
Score = 137 bits (346), Expect = 9e-33
Identities = 88/215 (40%), Positives = 123/215 (56%), Gaps = 13/215 (6%)
Query: 7 ILDIEGTVCPISFVKDTLYPFFLKQVESLVKTNDPTLQNLLAQFPVPQDASSLHEHIESL 66
+LDIEGTV ISFVKD L+P+ + ES V N + +NL P++A ++ L
Sbjct: 6 LLDIEGTVGSISFVKDKLFPYAASRYESYVNENYESDENLRELGKTPEEALI---NLRKL 62
Query: 67 VNNDIKDSVLKQLQGYIWEQGYKSGEIKAPVYPDAIDFIQRH---APNVYIYSSGSVKAQ 123
K+ K +QG IW++GY+S E+ + ++PD + IQR VYIYSSGSV AQ
Sbjct: 63 HAEGSKERSFKMVQGRIWKKGYESNELTSHLFPDVVPAIQRSLQLGMRVYIYSSGSVPAQ 122
Query: 124 ILLFQHVEGDIDLTKSIAGYFDINTSGKKTEPQSYTNILKSIGVPPSSASDVVFISDNDK 183
L F+H + +L K +GY+D T G KTE SY I V S+ + +F+SDN
Sbjct: 123 KLYFEHSDAG-NLLKYFSGYYD-TTIGLKTECGSYVKI-----VGNSNPREWLFLSDNIN 175
Query: 184 ELDAALDVGISTILALRPGNNPVSNAEKYKALNNF 218
EL AA VG+ T L +RPGN+PV + + N+F
Sbjct: 176 ELKAARKVGLHTGLVVRPGNDPVVDTSGFPVYNSF 210
>At5g53850_2 [E] KOG2630 Enolase-phosphatase E-1
Length = 240
Score = 124 bits (311), Expect = 1e-28
Identities = 83/243 (34%), Positives = 133/243 (54%), Gaps = 32/243 (13%)
Query: 7 ILDIEGTVCPISFVKDTLYPFFLKQVESLVKTNDPTLQN------LLAQF---------- 50
+LDIEGT PI+FV D L+P+ + V + T + L AQ
Sbjct: 2 VLDIEGTTTPITFVTDVLFPYARENVGKHLNLTYHTAETQEDIKLLRAQVEEDLREGVTG 61
Query: 51 --PVPQD-------ASSLHEHIESLVNNDIKDSVLKQLQGYIWEQGYKSGEIKAPVYPDA 101
P+P +++ ++E+++ D K + LK+LQG+IW G++ E+KA V+ D
Sbjct: 62 AVPIPHADEGKEKVIAAMVSNVEAMIRADRKITALKELQGHIWRTGFECDELKAIVFEDV 121
Query: 102 IDFIQR-HAP--NVYIYSSGSVKAQILLFQHVEGDIDLTKSIAGYFDINTSGKKTEPQSY 158
D +++ H+ VYIYSSGS AQ LLF + + DL K I+G+FD T G K E +SY
Sbjct: 122 ADALEKWHSSGIKVYIYSSGSRLAQKLLFGNTDYG-DLRKYISGFFD-TTIGNKKESRSY 179
Query: 159 TNILKSIGVPPSSASDVVFISDNDKELDAALDVGISTILALRPGNNPVSNAEKYKALNNF 218
I +++GV ++++F++D +E AA G+ I+++RPGN P+ +K + +F
Sbjct: 180 KEIKETLGV--DDPAEIMFVTDVYQEAVAAKAAGLEAIISIRPGNAPLPENHGFKTVTSF 237
Query: 219 SSI 221
S I
Sbjct: 238 SQI 240
>Hs10864017 [E] KOG2630 Enolase-phosphatase E-1
Length = 261
Score = 121 bits (303), Expect = 9e-28
Identities = 84/248 (33%), Positives = 134/248 (53%), Gaps = 39/248 (15%)
Query: 7 ILDIEGTVCPISFVKDTLYPFFLKQVESLVKTN--------DPTLQNLLAQ--------F 50
+LDIEGT PI+FVKD L+P+ + V+ ++T+ D +L A+
Sbjct: 14 LLDIEGTTTPIAFVKDILFPYIEENVKEYLQTHWEEEECQQDVSLLRKQAEEDAHLDGAV 73
Query: 51 PVP----QDASSLHEHIESLVNN-------DIKDSVLKQLQGYIWEQGYKSGEIKAPVYP 99
P+P L + I+++V+N D K + LKQLQG++W + +G +KA +
Sbjct: 74 PIPAASGNGVDDLQQMIQAVVDNVCWQMSLDRKTTALKQLQGHMWRAAFTAGRMKAEFFA 133
Query: 100 DAIDFIQR---HAPNVYIYSSGSVKAQILLFQH-VEGDIDLTKSIAGYFDINTSGKKTEP 155
D + +++ VYIYSSGSV+AQ LLF H EGDI + + G+FD G K E
Sbjct: 134 DVVPAVRKWREAGMKVYIYSSGSVEAQKLLFGHSTEGDI--LELVDGHFDTKI-GHKVES 190
Query: 156 QSYTNILKSIGVPPSSASDVVFISDNDKELDAALDVGISTILALRPGNNPVSNAEK--YK 213
+SY I SIG S ++++F++D +E AA + + + +RPGN +++ EK Y
Sbjct: 191 ESYRKIADSIG---CSTNNILFLTDVTREASAAEEADVHVAVVVRPGNAGLTDDEKTYYS 247
Query: 214 ALNNFSSI 221
+ +FS +
Sbjct: 248 LITSFSEL 255
>CE06027 [E] KOG2630 Enolase-phosphatase E-1
Length = 508
Score = 120 bits (300), Expect = 2e-27
Identities = 87/256 (33%), Positives = 128/256 (49%), Gaps = 51/256 (19%)
Query: 7 ILDIEGTVCPISFVKDTLYPFFLKQV-----------------ESLVKTNDPTLQNLLAQ 49
+LDIEGT+ ISFVKD L+P+ + V E L D +N +A
Sbjct: 12 LLDIEGTITSISFVKDELFPYAFENVGNYLEEHYDNPATQIIVEDLRHIADQQAENDVAV 71
Query: 50 FPVPQDASSLHEHIESLVNNDIKD--------------------SVLKQLQGYIWEQGYK 89
+ + E + V + IK + +K LQG IWE+ Y+
Sbjct: 72 VRIREPRKECIEDVTKNVRHWIKRDKKVYRVFEKRCLKFNIFQLTPMKALQGLIWEEAYQ 131
Query: 90 SGEIKAPVYPD---AIDFIQRHAPNVYIYSSGSVKAQILLFQH-VEGDIDLTKSIAGYFD 145
G++K VYPD + ++ +YIYSSGSV AQ LLF + +EG D+TK + GYFD
Sbjct: 132 RGDVKGHVYPDVLPVLKIVENRKIPIYIYSSGSVHAQKLLFANSIEG--DMTKILYGYFD 189
Query: 146 INTSGKKTEPQSYTNILKSIGVPPSSASDVVFISDNDKELDAALDVGISTILALRPGNNP 205
N G K E SYT I + I +PP S+++F++D + E AA G+ T L +RPGN
Sbjct: 190 TNI-GLKGESNSYTKISERIKIPP---SEILFLTDVEAEAAAAKKAGLQTKLVVRPGNAG 245
Query: 206 VSNAEKYKALNNFSSI 221
++ +A+N + +I
Sbjct: 246 LTQ----EAINAYGTI 257
>7296775 [E] KOG2630 Enolase-phosphatase E-1
Length = 252
Score = 101 bits (251), Expect = 9e-22
Identities = 77/226 (34%), Positives = 113/226 (49%), Gaps = 30/226 (13%)
Query: 7 ILDIEGTVCPISFVKDTLYPFFLKQVESL---------VKTNDPTLQN---------LLA 48
++DIEGT ISFV D L+P+ + VE +K LQ LL+
Sbjct: 12 LVDIEGTTTSISFVHDVLFPYAKQNVEKFLRDSWEEDDIKRIVQDLQQVPQYADYKALLS 71
Query: 49 QFPVPQDASSLHEHIESLVNNDIKDSVLKQLQGYIWEQGYKSGEIKAPVY---PDAIDFI 105
P D + + L++ D+K + +K LQG IW QGY +GE+K VY P A +
Sbjct: 72 GPPTEVDVDLIAGFVRYLIDQDLKVTPMKTLQGLIWAQGYANGELK--VYEDVPAAFEAW 129
Query: 106 QRHAPNVYIYSSGSVKAQILLFQHVEGDIDLTKSIAGYFDINTSGKKTEPQSYTNILKSI 165
+ + +YSSGSV AQ L+F H +L ++ YFD + G K E QSY NI K +
Sbjct: 130 RAAGLQIAVYSSGSVAAQKLIFGHSLAG-NLQPYLSAYFDTHV-GHKQEQQSYKNIAKQL 187
Query: 166 GVPPSSASDVVFISDNDKELDAALDVGISTILALRPGNNPVSNAEK 211
P ++F++ D E AA G+ I+ RPGN +++ +K
Sbjct: 188 KEDP---KQILFLT--DIEAAAARCAGLQAIILKRPGNAALADDQK 228
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.315 0.134 0.378
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,248,685
Number of Sequences: 60738
Number of extensions: 542720
Number of successful extensions: 1180
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 1158
Number of HSP's gapped (non-prelim): 7
length of query: 221
length of database: 30,389,216
effective HSP length: 102
effective length of query: 119
effective length of database: 24,193,940
effective search space: 2879078860
effective search space used: 2879078860
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)