ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV5843 suspect: LH YU KOG2271 Intracellular trafficking, secretion, and vesicular transport Nuclear pore complex component (sc Nup85)
r_klactV5843 suspect: LH YU KOG2271 Nuclear structure Nuclear pore complex component (sc Nup85)
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV5843 2003178 2001007 -724
(724 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YJR042w [YU] KOG2271 Nuclear pore complex component (sc Nup85) 588 e-168
SPBC17G9.04c [YU] KOG2271 Nuclear pore complex component (sc Nup85) 87 1e-16
>YJR042w [YU] KOG2271 Nuclear pore complex component (sc Nup85)
Length = 744
Score = 588 bits (1517), Expect = e-168
Identities = 307/744 (41%), Positives = 471/744 (63%), Gaps = 31/744 (4%)
Query: 8 DTQDLLMDVDNLDFLDE--EGISNESDDDINLTINVDPVSNAPMVNFPI-EQPSAKE--- 61
D+ LLMDVD DFLD+ +SN D+ DPVS A +V + +QP K
Sbjct: 5 DSNRLLMDVDQFDFLDDGTAQLSNNKTDEEEQLYKRDPVSGAILVPMTVNDQPIEKNGDK 64
Query: 62 --LRFKYNNVSSRSLAFDNSTKDNKLYPVRFLHLDESKEFAQYVSKLFELYQGLGEHRKN 119
L+FK +S +++AF + KLYPVR LD SKEF+ YVS LFE+Y+ LG+ R
Sbjct: 65 MPLKFKLGPLSYQNMAFITAKDKYKLYPVRIPRLDTSKEFSAYVSGLFEIYRDLGDDRVF 124
Query: 120 DVPTIGLIKQTSRLEHFSIVNLAFHALVTELEFYIESIK-YTNKLQRIGDLEECLSILNC 178
+VPTIG++ EH + VNLA A++ ELE +I +K ++ R +LEE L++LNC
Sbjct: 125 NVPTIGVVNSNFAKEHNATVNLAMEAILNELEVFIGRVKDQDGRVNRFYELEESLTVLNC 184
Query: 179 LKTIYFLTDSPEYKQE--DLLESLINWVNRSDGEPSELVIQKIFDETLLTR-RKVYEQSD 235
L+T+YF+ D + ++ + +ESL+NW+NRSDGEP E I+++F T +KV+E
Sbjct: 185 LRTMYFILDGQDVEENRSEFIESLLNWINRSDGEPDEEYIEQVFSVKDSTAGKKVFETQY 244
Query: 236 FWDLTAQLLLRGLWSQSIQCIQNSQLLEHGNETVGVF---VTDLITIIESYPLHSENLFR 292
FW L QL+LRGL SQ+I CI+ S LL + ++T V V+D I +++ YP S + FR
Sbjct: 245 FWKLLNQLVLRGLLSQAIGCIERSDLLPYLSDTCAVSFDAVSDSIELLKQYPKDSSSTFR 304
Query: 293 EWKNSVLQLMTNWDEQ--EVEPEIKKNLMNIFMILSGSKNKICEFSQYWYESYCGLMLYY 350
EWKN VL+L + ++ E++ + + +++ G++ KI ++S+ WYES+CG +LYY
Sbjct: 305 EWKNLVLKLSQAFGSSATDISGELRDYIEDFLLVIGGNQRKILQYSRTWYESFCGFLLYY 364
Query: 351 IPTLELSQEYGQLATKHNAIDVCNNWETPCYHIINDKVADVLPILESLDLATAAFVAVLC 410
IP+LELS EY Q++ + N +D+ N+WE PC II+ K+ +LP++ESLD TAAF A++C
Sbjct: 365 IPSLELSAEYLQMSLEANVVDITNDWEQPCVDIISGKIHSILPVMESLDSCTAAFTAMIC 424
Query: 411 EAKGIIKTDFY-----EIFAHKSGLNSELAFENEPTIGEFLIRQVALSLCSYDDKTLWPX 465
EAKG+I+ F + ++++ E F + +++ A LCS DK LWP
Sbjct: 425 EAKGLIENIFEGEKNSDDYSNEDNEMLEDLFSYRNGMASYMLNSFAFELCSLGDKELWPV 484
Query: 466 XXXXXXXXXXXXXXXKRAILAELLPHFPYTTNDDIEWLLSVCAKWRLPDVAKTIYKILGQ 525
K+ ++AELLPH+P+ TNDDIEW+LS+C +WRLP++AK IY LG
Sbjct: 485 AIGLIALSATGTRSAKKMVIAELLPHYPFVTNDDIEWMLSICVEWRLPEIAKEIYTTLGN 544
Query: 526 DTLYQNNIVEAMSNFSKAGEYEWVKHYSWMIFEASILQGGPLDDITINSIVNGDT---DS 582
L +NI+E+++NFS+AG+YE VK YSW++FEAS ++G LDD +N+IV+ ++ D
Sbjct: 545 QMLSAHNIIESIANFSRAGKYELVKSYSWLLFEASCMEGQKLDDPVLNAIVSKNSPAEDD 604
Query: 583 FNIPKDLLNSMVTDVMRQTLSPYAVLYEFHKLAENNETEKAMNHMIQLISFPYMPEQYCI 642
IP+D+L+ +VT+ MRQTL+PYAVL +F++L + + +A+ ++ LI FPY+P+ Y +
Sbjct: 605 VIIPQDILDCVVTNSMRQTLAPYAVLSQFYELRDREDWGQALRLLLLLIEFPYLPKHYLV 664
Query: 643 LLLAKFFYPMFLKTTETALDESQIFTILKTLEK--IKLNDSCVALYSQLRNDDNSTCSNL 700
LL+AKF YP+FL + +DE + T+++ +E ++ LY + D S L
Sbjct: 665 LLVAKFLYPIFLLDDKKLMDEDSVATVIEVIETKWDDADEKSSNLYETIIEADKS----L 720
Query: 701 PESVDSFIQDVRKTLNYKICQSFM 724
P S+ + ++++RK LN+K+CQ+FM
Sbjct: 721 PSSMATLLKNLRKKLNFKLCQAFM 744
>SPBC17G9.04c [YU] KOG2271 Nuclear pore complex component (sc Nup85)
Length = 675
Score = 86.7 bits (213), Expect = 1e-16
Identities = 148/701 (21%), Positives = 278/701 (39%), Gaps = 115/701 (16%)
Query: 30 ESDDDINLTINVDPVSNAPMVNFPIE--QPSAKELRFKYNNVSSRSLAFDNSTKDNKLYP 87
ES D+++L + + NAP+ N + + + + FK + + LA+ N NK +
Sbjct: 2 ESSDEVDLPVY--HLQNAPIENGKLNDWRSKGRTVAFKLHPFLRKGLAYIN----NKEFD 55
Query: 88 VRFLHLDESKEFAQYVSK------------LFELYQGLGEHRKNDVPTIGLIKQTSRLEH 135
L D+ +E V + L + L E ++ T L + + L
Sbjct: 56 ENTLKSDKFEEVESLVYEFSTPSTLIEPNYLLTAWHELWEELQDTYMTPILDSEANLL-- 113
Query: 136 FSIVNLAFHALVTELEFYIESIKYTNKLQ-RIGDLE-ECLSILN----------CLKTIY 183
+ F+ ++ L F + I+ +LQ RI + E +C +L+ C + IY
Sbjct: 114 ---LLTQFYGKISSL-FRSKIIQVLEELQSRINEGEKDCQPVLDSLWEVESAWRCAEAIY 169
Query: 184 FLTDSPEYKQEDLLESLINWVNRSDGEPSELVIQKIFDETL--LTRRKVYEQSDFWDLTA 241
F SP L +++WVN D +P I D+ L + R Y+ +FW
Sbjct: 170 FPPSSPY----TLSTGILDWVNAYDPQP-------IADDGLEIMAYRIPYQHPEFWPYVN 218
Query: 242 QLLLRGLWSQSIQCIQNSQLLEHGNETVGVFVTDLITIIESYPLHSENLFRE-------- 293
+ +RGL+ Q+I C++ S L + + V +LI I+ P + R
Sbjct: 219 KTAIRGLFEQTISCLEMSGLTKEW-PVLKETVDELIDILRYSPCTHQKRIRSVSDFERRW 277
Query: 294 --WKNSVLQLM-TNWDEQEVEPEIKKNLMNIFMILSGSKNKICEFSQYWYESYCGLMLYY 350
W++ + L ++++ E+ + + + IL+G+K I +W E + L Y
Sbjct: 278 KLWRSRLANLRHVVKKHRDIDSEVLDDFVVLLDILNGNKEVIMLSCAHWQEYFSALAFLY 337
Query: 351 IPT-----LELSQEYGQLATKHNAIDVCNN---WETPCYHIINDKVADVLPILESLDLAT 402
P ++S Y QLAT ++ N +E C ++ +++ + + LDL
Sbjct: 338 GPLDCKNPEDISLLY-QLATGEDSKFYVNGTIEYEQICVNLCSNEPLNAIKHAYLLDLGL 396
Query: 403 AAFVAVLCEAKGIIKTDFYEIFAHKSGLNSELAFENEPTIGEFLIRQVALSLCSYDDKTL 462
A +A L G ++ E E T+ E LI + C + + L
Sbjct: 397 AVHLADLLSKSGHLRDYITE--------------EYPITLREHLILEYGQ--CVLESRNL 440
Query: 463 WPXXXXXXXXXXXXXXXXKRAILAELLPHFPYTTNDDIEWLLSVCAKWRLPDVAKTIYKI 522
W +A + P+ P + D E L +C + +L D A+ +
Sbjct: 441 WQTSFAYWKCVADSGYQRIKACI----PYVPLSDVDAKETALQLCKQLKLRDEAQLVLTH 496
Query: 523 LGQDTLYQNNIVEAMSNFSKAGEYEWVKHYSWMIFEASILQG---GPLDDITINSIVNGD 579
+ + +N+ EA+ A Y + +W +F+ I + P D + +
Sbjct: 497 WADELIARNHYGEALIALDNAANYSALNRVTWELFDICIAEKKSFSPDKDELLYEL---- 552
Query: 580 TDSFNIPKDLLNSMVTDVMRQTLSPYAVLYEF-----HKLAENNETEKAMNHMIQLISFP 634
F+ PK T + +SP A ++++ HK E N +E + ++ ++ FP
Sbjct: 553 ---FSSPK-----ACTPTLASIISPAATIHQYFFYLQHK-KELNASELLVG-LLTMVDFP 602
Query: 635 YMPEQYCILLLAKFF-YPMFLKTTETALDESQIFTILKTLE 674
+ LL +F P+ +T+ L ++ + L+
Sbjct: 603 SSRFPKLLELLHEFLNNPLQSNSTDFKLSLVNVYDCIAVLQ 643
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.319 0.136 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 42,780,835
Number of Sequences: 60738
Number of extensions: 1814713
Number of successful extensions: 4932
Number of sequences better than 1.0e-05: 2
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 4920
Number of HSP's gapped (non-prelim): 2
length of query: 724
length of database: 30,389,216
effective HSP length: 114
effective length of query: 610
effective length of database: 23,465,084
effective search space: 14313701240
effective search space used: 14313701240
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)