ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV5974 suspect: LH R KOG1454 General function prediction only Predicted hydrolase/acyltransferase (alpha/beta hydrolase superfamily)
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV5974 2051146 2052120 325
(325 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
At5g17780 [R] KOG1454 Predicted hydrolase/acyltransferase (alpha... 52 1e-06
At5g38520 [R] KOG1454 Predicted hydrolase/acyltransferase (alpha... 50 4e-06
>At5g17780 [R] KOG1454 Predicted hydrolase/acyltransferase (alpha/beta
hydrolase superfamily)
Length = 419
Score = 52.0 bits (123), Expect = 1e-06
Identities = 63/267 (23%), Positives = 104/267 (38%), Gaps = 25/267 (9%)
Query: 72 YKSKSVDKLVLMIHGLGGNLTHYEPLISKYVH-DHTPFLAFDLPGFGDSDELDQ--YNMG 128
Y + + V+ IHG G+ + + +++ D LA DL GFG+S + Y +
Sbjct: 143 YSVQEPSENVIFIHGFMGSSHFWTETVFEHIQKDDYRLLAIDLLGFGESPKPRDSLYTLK 202
Query: 129 DVISLICELVHKMCQCKTISIIGHSMGALLSVQVANTISVKCHGLILIGTPPLQNKALKN 188
D + I V K Q + ++ HSMG L+++ +A S + L+ P + +
Sbjct: 203 DHVDTIERSVIKPYQLDSFHVVAHSMGCLIALALAAKHSNIVKSVTLVAPPYFPSSVEGS 262
Query: 189 PFVRLLLKLLWYHPGIFDFYRVRFDQSKGLKSSGIVKFYYRAGNTYGKLYQYYRNIQIKS 248
R+ K LW P F + + + G I+ +++ KL R I K
Sbjct: 263 VLNRIARKRLW-PPLAFGTAVMSWYEHIGRCVCFIICKHHKIWEWLIKLCIGKREIHWKI 321
Query: 249 KSLV--GYFLGWQEVSNV----------------PNATPIHLIHGDKDTICAVEKLAQW- 289
K + + W + NV + IHL+ GD D I
Sbjct: 322 KDITRHTHHSAWHSMHNVICGGSKVADEHLETLIKSGVKIHLMQGDCDQIVPSHCSGNMK 381
Query: 290 RTLEGVTVTVDVITDCSHNCLLDATEE 316
RT V VD+IT H+ ++ E
Sbjct: 382 RTFP--AVEVDIITGADHDSMISGRGE 406
>At5g38520 [R] KOG1454 Predicted hydrolase/acyltransferase (alpha/beta
hydrolase superfamily)
Length = 362
Score = 50.1 bits (118), Expect = 4e-06
Identities = 41/123 (33%), Positives = 68/123 (54%), Gaps = 7/123 (5%)
Query: 81 VLMIHGLGGNLTHYEPLISKYVHDHTPFLAFDLPGFGDSDELD--QYNMGDVISLICELV 138
VL++HG G ++ H+ I+ +HT + A DL GFG SD+ Y M LI +
Sbjct: 93 VLLVHGFGASIPHWRRNINALSKNHTVY-AIDLLGFGASDKPPGFSYTMESWAELILNFL 151
Query: 139 HKMCQCKTISIIGHSMGALLSVQVAN-TISVKCHGLILIG-TPPLQNKALKNPF-VRLLL 195
++ Q TI +IG+S+G+L V A+ + GL+L+ + NKA+ + + ++LL+
Sbjct: 152 EEVVQKPTI-LIGNSVGSLACVIAASESRGDLVKGLVLLNCAGGMNNKAVFDDWRIKLLM 210
Query: 196 KLL 198
LL
Sbjct: 211 PLL 213
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.322 0.140 0.426
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 20,185,197
Number of Sequences: 60738
Number of extensions: 852300
Number of successful extensions: 1988
Number of sequences better than 1.0e-05: 2
Number of HSP's better than 0.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 1988
Number of HSP's gapped (non-prelim): 2
length of query: 325
length of database: 30,389,216
effective HSP length: 107
effective length of query: 218
effective length of database: 23,890,250
effective search space: 5208074500
effective search space used: 5208074500
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)