ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV6006 good U KOG3284 Intracellular trafficking, secretion, and vesicular transport Vacuolar sorting protein VPS28
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV6006 2062060 2061323 -246
(246 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YPL065w [U] KOG3284 Vacuolar sorting protein VPS28 244 1e-64
SPAC1B3.07c [U] KOG3284 Vacuolar sorting protein VPS28 135 4e-32
At4g21560 [U] KOG3284 Vacuolar sorting protein VPS28 117 1e-26
At4g05000 [U] KOG3284 Vacuolar sorting protein VPS28 114 2e-25
7304105 [U] KOG3284 Vacuolar sorting protein VPS28 111 8e-25
Hs7705885 [U] KOG3284 Vacuolar sorting protein VPS28 110 2e-24
CE23139 [U] KOG3284 Vacuolar sorting protein VPS28 99 5e-21
>YPL065w [U] KOG3284 Vacuolar sorting protein VPS28
Length = 242
Score = 244 bits (622), Expect = 1e-64
Identities = 122/235 (51%), Positives = 170/235 (71%), Gaps = 4/235 (1%)
Query: 15 NSTRPLNPKLLEEIQPFDLNAQPREKEIVDTLGEIYSIIITLDHVEKAFLKDDIGSEEYT 74
N + ++ +E+ FD + ++KE+++TL EIYSI+ITLDHVEKA+LKD I +YT
Sbjct: 9 NQNQDISQLFHDEVPLFDNSITSKDKEVIETLSEIYSIVITLDHVEKAYLKDSIDDTQYT 68
Query: 75 RLANKLINQYRTYLSDEDVEE---QFKSLDHFKAKWQITASNAITRLERGIPVTVEHGTS 131
+KL+ Q++ YL+ ++ EE F+S++ F + ITASNAITRLERGIP+T EH S
Sbjct: 69 NTVDKLLKQFKVYLNSQNKEEINKHFQSIEAFCDTYNITASNAITRLERGIPITAEHAIS 128
Query: 132 GDTDSLDNSRAADNTTNLNKYSGKRVAEATGNFITVMDALKLNYKTREQLHPLMAELLLS 191
T + ++++ K++ K VAEATGNFITVMDALKLNY ++QLHPL+AELL+S
Sbjct: 129 TTTSAPSGDNKQSSSSD-KKFNAKYVAEATGNFITVMDALKLNYNAKDQLHPLLAELLIS 187
Query: 192 INRVTSNDFENRSKLVEWIVKINKLPKDEQLSDADSQQLLYSLESAYKSFYTLLE 246
INRVT +DFENRSKL++WIV+INKL + L++ ++LL+ LE AYKSFY LL+
Sbjct: 188 INRVTRDDFENRSKLIDWIVRINKLSIGDTLTETQIRELLFDLELAYKSFYALLD 242
>SPAC1B3.07c [U] KOG3284 Vacuolar sorting protein VPS28
Length = 248
Score = 135 bits (341), Expect = 4e-32
Identities = 80/242 (33%), Positives = 137/242 (56%), Gaps = 22/242 (9%)
Query: 24 LLEEIQPFDLNAQPREKEIVDTLGEIYSIIITLDHVEKAFLKDDIGSEEYTRLANKLINQ 83
LLE+ + N + +++ + L +YSI++ L+ +EKAF KD + + ++ LI Q
Sbjct: 9 LLEKETSEENNFHTKNQQVREDLSILYSILVALEQLEKAFTKDAVSTSDFNSTCELLIQQ 68
Query: 84 YRTYLSDEDVEEQFKSLDHFKAKWQITASNAITRLERGIPVTVEHGTSGDTDS------L 137
+ + SDE V + F S + F +K+++ AI R++ GI + E S T S
Sbjct: 69 WESCFSDERVTQAFGSFEDFCSKYRLQCPRAIKRIQEGI--SDERSQSNSTFSNAISTTA 126
Query: 138 DNSRAADNTT-----------NLNKYSGKRVAEATGNFITVMDALKLNYKTREQLHPLMA 186
+ S A ++TT N + K +A NFIT +DA++LN+ ++QLHPL++
Sbjct: 127 EPSIAMNDTTPQTVNPTKAPSNPSASIAKSIAGLVQNFITTLDAIRLNFIAKDQLHPLLS 186
Query: 187 ELLLSINRVTSN---DFENRSKLVEWIVKINKLPKDEQLSDADSQQLLYSLESAYKSFYT 243
EL++S++ +T + R+KLV+W++KIN + +QL+D + ++LLY LE AY Y+
Sbjct: 187 ELIVSMDDLTESLKIQVSCRNKLVQWLIKINNMNITDQLNDVEKRELLYDLEQAYAECYS 246
Query: 244 LL 245
LL
Sbjct: 247 LL 248
>At4g21560 [U] KOG3284 Vacuolar sorting protein VPS28
Length = 209
Score = 117 bits (294), Expect = 1e-26
Identities = 69/211 (32%), Positives = 123/211 (57%), Gaps = 19/211 (9%)
Query: 38 REKEIVDTLGEIYSIIITLDHVEKAFLKDDIGSEEYTRLANKLINQYRTYLSDEDVEEQF 97
RE+E+ + E+Y+II + +EKA+++D I EY KLI ++T + +++
Sbjct: 10 REREMYENFAELYAIIKATEKLEKAYIRDLISPSEYETECQKLIVHFKTLSAS--LKDMV 67
Query: 98 KSLDHFKAKWQITASNAITRL-ERGIPVTVEHGTSGDTDSLDNSRAADNTTNLNKYSGKR 156
+++ F +++ S A+ RL G+P TVEH RAA + + + S
Sbjct: 68 PNIERFAETYKMDCSAAVYRLVTSGVPATVEH------------RAAASASTSS--SASV 113
Query: 157 VAEATGNFITVMDALKLNYKTREQLHPLMAELLLSINRVT--SNDFENRSKLVEWIVKIN 214
VAE NFIT MD+LKLN +Q++PL+++L S+N+++ DFE + K+ EW+++++
Sbjct: 114 VAECVQNFITSMDSLKLNMVAVDQVYPLLSDLSASLNKLSILPPDFEGKIKMKEWLLRLS 173
Query: 215 KLPKDEQLSDADSQQLLYSLESAYKSFYTLL 245
K+ ++L++ ++QL + LES+Y SF L
Sbjct: 174 KMGASDELTEQQARQLHFDLESSYNSFMAAL 204
>At4g05000 [U] KOG3284 Vacuolar sorting protein VPS28
Length = 209
Score = 114 bits (284), Expect = 2e-25
Identities = 68/211 (32%), Positives = 122/211 (57%), Gaps = 19/211 (9%)
Query: 38 REKEIVDTLGEIYSIIITLDHVEKAFLKDDIGSEEYTRLANKLINQYRTYLSDEDVEEQF 97
RE+E+ + E+++II + +EKA+++D I EY KLI ++T + +++
Sbjct: 10 REREMYENFAELFAIIKATEKLEKAYIRDLINPSEYESECQKLIVHFKTLSAT--LKDTV 67
Query: 98 KSLDHFKAKWQITASNAITRL-ERGIPVTVEHGTSGDTDSLDNSRAADNTTNLNKYSGKR 156
+++ F +++ A+ RL G+P TVEH + A +T+N S
Sbjct: 68 PNIERFADTYKMDCPAALYRLVTSGLPATVEH----------RATVAASTSN----SASI 113
Query: 157 VAEATGNFITVMDALKLNYKTREQLHPLMAELLLSINRVT--SNDFENRSKLVEWIVKIN 214
VAE NFIT MD+LKLN +Q++PL+++L S+N+++ DFE ++K+ EW+ +++
Sbjct: 114 VAECVQNFITSMDSLKLNMVAVDQVYPLLSDLSASLNKLSILPPDFEGKTKMKEWLSRLS 173
Query: 215 KLPKDEQLSDADSQQLLYSLESAYKSFYTLL 245
K+ ++L++ S+QL + LES+Y SF L
Sbjct: 174 KMGAADELTEQQSRQLHFDLESSYNSFMAAL 204
>7304105 [U] KOG3284 Vacuolar sorting protein VPS28
Length = 210
Score = 111 bits (278), Expect = 8e-25
Identities = 70/227 (30%), Positives = 123/227 (53%), Gaps = 26/227 (11%)
Query: 21 NPKLLEEIQPFDLNAQPREKEIVDTLGEIYSIIITLDHVEKAFLKDDIGSEEYTRLANKL 80
+P+L EE++ F NA+ REK D + ++Y+II T+ +EKA+++D I +EYT +K
Sbjct: 5 SPELYEEVKLFR-NAREREK--YDNMADLYAIINTIQQLEKAYIRDCITPQEYTAACSKY 61
Query: 81 INQYRTYLSDEDVEEQFKSLDHFKAKWQITASNAITRLERGIPVTVEHGTSGDTDSLDNS 140
+ QY+ +E F S++ F K+++ A+ R+ P+T+
Sbjct: 62 LVQYKVAFKQVQCDE-FPSVETFVKKFRLDCPAALERIREDRPITIR------------- 107
Query: 141 RAADNTTNLNKYSGKRVAEATGNFITVMDALKLNYKTREQLHPLMAELLLSINRVT--SN 198
D+ N + K +AE FIT+MD L+L T + L P + +L ++NR++
Sbjct: 108 ---DDKGN----TSKCIAEIVSLFITIMDKLRLQINTMDALQPDVKDLADNMNRLSLIPE 160
Query: 199 DFENRSKLVEWIVKINKLPKDEQLSDADSQQLLYSLESAYKSFYTLL 245
DF+ + K+ +W+ +N++ ++LS+ +Q L+ LESAY F LL
Sbjct: 161 DFDAKLKVEKWLGSLNEMQASDELSEGQVRQFLFDLESAYADFNKLL 207
>Hs7705885 [U] KOG3284 Vacuolar sorting protein VPS28
Length = 221
Score = 110 bits (274), Expect = 2e-24
Identities = 64/226 (28%), Positives = 122/226 (53%), Gaps = 26/226 (11%)
Query: 22 PKLLEEIQPFDLNAQPREKEIVDTLGEIYSIIITLDHVEKAFLKDDIGSEEYTRLANKLI 81
P+L EE++ L RE+E D + E+++++ T+ +EKA++KD + EYT ++L+
Sbjct: 18 PELYEEVK---LYKNAREREKYDNMAELFAVVKTMQALEKAYIKDCVSPSEYTAACSRLL 74
Query: 82 NQYRTYLSDEDVEEQFKSLDHFKAKWQITASNAITRLERGIPVTVEHGTSGDTDSLDNSR 141
QY+ E S+D F K+++ A+ R++ P+T++
Sbjct: 75 VQYKAAFRQVQGSE-ISSIDEFCRKFRLDCPLAMERIKEDRPITIK-------------- 119
Query: 142 AADNTTNLNKYSGKRVAEATGNFITVMDALKLNYKTREQLHPLMAELLLSINRVT--SND 199
D+ NLN+ +A+ FITVMD L+L + +++ P + EL+ +++R++ D
Sbjct: 120 --DDKGNLNRC----IADVVSLFITVMDKLRLEIRAMDEIQPDLRELMETMHRMSHLPPD 173
Query: 200 FENRSKLVEWIVKINKLPKDEQLSDADSQQLLYSLESAYKSFYTLL 245
FE R + +W+ ++ + ++L D+ +Q+L+ LESAY +F L
Sbjct: 174 FEGRQTVSQWLQTLSGMSASDELDDSQVRQMLFDLESAYNAFNRFL 219
>CE23139 [U] KOG3284 Vacuolar sorting protein VPS28
Length = 210
Score = 99.0 bits (245), Expect = 5e-21
Identities = 59/223 (26%), Positives = 123/223 (54%), Gaps = 28/223 (12%)
Query: 21 NPKLLEEIQPFDLNAQPREKEIVDTLGEIYSIIITLDHVEKAFLKDDIGSEEYTRLANKL 80
N L+ E++ F+ ++ E+E ++ L E+++++ L+H+EK F +D + ++EY KL
Sbjct: 5 NANLMREVRLFENHS---EREQMENLSELFAVLNALEHLEKMFSRDHVSADEYKSECFKL 61
Query: 81 INQYRTYLSDEDVEEQFKSLDHFKAKWQITASNAITRLERGIPVTVEHGTSGDTDSLDNS 140
I+QY+ + + S++ F K+++ AI R+ G P+TV+
Sbjct: 62 IDQYKVTMR---LVHGATSIEDFAKKYRLHCPAAIERIREGRPITVK------------- 105
Query: 141 RAADNTTNLNKYSGKRVAEATGNFITVMDALKLNYKTREQLHPLMAELLLSINRVTSNDF 200
D+ N+ K+ +A FIT +DAL+LN + + L+P + +L +IN +
Sbjct: 106 ---DDQGNVLKH----IASIVEQFITFLDALRLNTRAVDDLYPGLDDLYNAINTTSRVPI 158
Query: 201 EN--RSKLVEWIVKINKLPKDEQLSDADSQQLLYSLESAYKSF 241
+ +K+ +W +++ + +++SD D++Q+++ +ESAY++F
Sbjct: 159 DAIVTTKVKKWHDRLSSMAASDEISDEDARQMIFDVESAYQAF 201
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.313 0.131 0.360
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,562,951
Number of Sequences: 60738
Number of extensions: 607562
Number of successful extensions: 1524
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 1507
Number of HSP's gapped (non-prelim): 7
length of query: 246
length of database: 30,389,216
effective HSP length: 104
effective length of query: 142
effective length of database: 24,072,464
effective search space: 3418289888
effective search space used: 3418289888
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)