ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactV6046    suspect: Pn   U     KOG2319  Intracellular trafficking, secretion, and vesicular transport  Vacuolar assembly/sorting protein VPS9

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactV6046 2075762 2074224 -513 
         (513 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC29A10.11c [U] KOG2319 Vacuolar assembly/sorting protein VPS9       61   5e-09
YML097c [U] KOG2319 Vacuolar assembly/sorting protein VPS9             55   2e-07
At3g19770 [U] KOG2319 Vacuolar assembly/sorting protein VPS9           52   3e-06

>SPBC29A10.11c [U] KOG2319 Vacuolar assembly/sorting protein VPS9
          Length = 402

 Score = 60.8 bits (146), Expect = 5e-09
 Identities = 82/342 (23%), Positives = 144/342 (41%), Gaps = 48/342 (14%)

Query: 176 LLTPEEISHQIKLQEANNIKTDQIMDLCE---CE-IFKKILQVGTCVPNHPTRQAKSTSS 231
           LL PEEI  Q K ++    + D+ MD  E   CE ++ +I  + T          K   +
Sbjct: 91  LLFPEEIDAQKKARQHLLNQKDEWMDQIEDIVCEYLYDRIFCLSTSTDAAKDDLLKKFIA 150

Query: 232 NEWKMTSLFRNNPEFIEFDKMLNTKLNTMKELSSSRILDLPAFLALELKHKERLDMVGLL 291
           +E K   +   N   I  D+ L  +L+ + E          AF AL+ +H  R  +    
Sbjct: 151 SEEKKELI---NCIPIPDDEKLTNRLHEVSE----------AFFALDEQHTPRSKI---- 193

Query: 292 FDKMLHENVSPCDKVHCLIVLHDKLLALDQNNADEILPSMIYLLITNPRKELFLNLQFIK 351
            +  +  N S         +L+   L  ++ NAD +L   IY ++  P   L  +L F+ 
Sbjct: 194 -NTFMTVNSS---------ILNASQLPQEELNADSLLNLTIYCILCYPGFHLISHLNFVL 243

Query: 352 LFRFHRRLKEKELYVTTNFEAAISFI----DSLTTKDIITYCQHTDELKLPHLESISEQV 407
            FR    L  ++ Y  T FEAA++FI     +L T+  I        L++ + E++S   
Sbjct: 244 RFRNADFLSGEQRYCLTTFEAALTFILRACPNLLTQSSIQPSDDPLSLEVANSETVSTSN 303

Query: 408 KLPLATEESFIPDFKRSNSLSDLISVGNVIDSGFRNLMGRIKTYTXXXXXXXXHVAQIQV 467
            L    + S  P     +SLS+L ++G  ++  + +L+ ++  +T        +      
Sbjct: 304 SL---HDPSAEPYPVNRSSLSNLRNLGLALEKSYASLLSKVANHTAKSSEDSSN------ 354

Query: 468 PLPSIEVTEGPATDWRRYKDYKFEDLKVSDMQSIFDIYRDLA 509
              S+E    P  + R        DLK+ +++ +   Y+ LA
Sbjct: 355 ---SVEFVGDPPLE-RFLTVSDASDLKIGEIELLLSDYKRLA 392


>YML097c [U] KOG2319 Vacuolar assembly/sorting protein VPS9
          Length = 451

 Score = 55.5 bits (132), Expect = 2e-07
 Identities = 43/158 (27%), Positives = 78/158 (49%), Gaps = 11/158 (6%)

Query: 301 SPCDKVHCLIVLHDKLLAL------DQNNADEILPSMIYLLITNPRKELFLNLQFIKLFR 354
           SP DK+ C++     +  L      +QN AD  +P +IY ++    + L  N+ +I+ FR
Sbjct: 221 SPRDKMVCVLNASKVIFGLLKHTKLEQNGADSFIPVLIYCILKGQVRYLVSNVNYIERFR 280

Query: 355 FHRRLKEKELYVTTNFEAAISFIDSLTTKDIITYCQHTD--ELKLPHLESISEQVKLPLA 412
               ++ +E Y  ++ +AA++FI +LT +  +T   H D  E    + + ++E+ +    
Sbjct: 281 SPDFIRGEEEYYLSSLQAALNFIMNLTERS-LTIEDHEDFEEAYQRNFKQLAEEKEEEEK 339

Query: 413 TEESFIPDFKRSNS--LSDLISVGNVIDSGFRNLMGRI 448
            ++  IPD  + N   L  L  V N++ S F  L   I
Sbjct: 340 KKQLEIPDELQPNGTLLKPLDEVTNIVISKFNELFSPI 377


>At3g19770 [U] KOG2319 Vacuolar assembly/sorting protein VPS9
          Length = 472

 Score = 51.6 bits (122), Expect = 3e-06
 Identities = 46/157 (29%), Positives = 80/157 (50%), Gaps = 11/157 (7%)

Query: 237 TSLFRNNPEFIEFDKMLNTKLNTMKELSSSRILDL-PAFL-----ALELKHKERLDMVGL 290
           T +F +N E +  D+ L  K++ +++  S   LD+ P F       L  K  ++++M   
Sbjct: 44  TRVFASNTEEVIADEKLFQKMSLVQQFISPENLDIQPTFQNESSWLLAQKELQKINMYKA 103

Query: 291 LFDKMLHENVSPCDKVHCLIVLHDKLLALDQN--NADEILPSMIYLLITNPRKELFLNLQ 348
             DK++   +  C KV   ++L+  + A ++N   ADE LP +IY+ I     +L  NL 
Sbjct: 104 PRDKLVC--ILNCCKVINNLLLNASI-ASNENAPGADEFLPVLIYVTIKANPPQLHSNLL 160

Query: 349 FIKLFRFHRRLKEKELYVTTNFEAAISFIDSLTTKDI 385
           +I+ +R   +L  +  Y  TN  +A SFI ++  K I
Sbjct: 161 YIQRYRRESKLVGEAAYFFTNILSAESFISNIDAKSI 197


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.319    0.134    0.381 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 27,016,227
Number of Sequences: 60738
Number of extensions: 1041293
Number of successful extensions: 2802
Number of sequences better than 1.0e-05: 3
Number of HSP's better than  0.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 2800
Number of HSP's gapped (non-prelim): 5
length of query: 513
length of database: 30,389,216
effective HSP length: 111
effective length of query: 402
effective length of database: 23,647,298
effective search space: 9506213796
effective search space used: 9506213796
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)