ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV6174 suspect: LH J KOG4772 Translation, ribosomal structure and biogenesis Predicted tRNA-splicing endonuclease subunit
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactV6174 2119396  2120715 440  
         (440 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YPL083c [J] KOG4772 Predicted tRNA-splicing endonuclease subunit      287   2e-77
SPCC613.09 [J] KOG4772 Predicted tRNA-splicing endonuclease subunit   109   7e-24

>YPL083c [J] KOG4772 Predicted tRNA-splicing endonuclease subunit
          Length = 467

 Score =  287 bits (735), Expect = 2e-77
 Identities = 163/461 (35%), Positives = 259/461 (55%), Gaps = 47/461 (10%)

Query: 8   FNDSDVEGDELLQDWSLATKIIGDNKNV-IPLRGEKDYEPDGTGMQGSMLFKAKKVMFDV 66
           F + + E +EL QDWS    ++  N  + +P RGEKDYEPDGT +Q  +L+ A K MFD 
Sbjct: 18  FEEEEEEEEELQQDWSQLASLVSKNAALSLPKRGEKDYEPDGTNLQDLLLYNASKAMFDT 77

Query: 67  LRQPDRGTIIKNQVKAYYDLDLHVATIKFPKGTFTNTVGSPNSDGTLTLQFHEFVYLVER 126
           +    RGT +K++V+ YY    H A +  PKG+F  T+G  +S G L L FHEFVYL ER
Sbjct: 78  ISDSIRGTTVKSEVRGYYVPHKHQAVLLKPKGSFMQTMGRADSTGELWLDFHEFVYLAER 137

Query: 127 GSVSPIIDLGINGEKE-----EYLLSLQDVYSFFKSQDELDEFFVYAYLKRMGYIVFPTN 181
           G++ P   L     K      E LLS++D+YS F SQ E+D++FV+A+LKR+G+I+ P+N
Sbjct: 138 GTILPYYRLEAGSNKSSKHETEILLSMEDLYSLFSSQQEMDQYFVFAHLKRLGFILKPSN 197

Query: 182 WKYGKQLSVYSRYNKGENRFQNTYLLLNGI----FNLFTNISYSWLKDWATNINNIISFH 237
            +   + S +    +  N    T+ LL+       +LF+   YS    W        +F 
Sbjct: 198 QEAAVKTSFFPLKKQRSNLQAITWRLLSLFKIQELSLFSGFFYS---KW--------NFF 246

Query: 238 PVKFASSGAIFESINKTITFYAPPKSQKQLLESHGVDLNAPVSSNYKIAFDVWKPNPSFK 297
             K+ +S  +++ +N+ +   A PK++K+LL++   D       +  + F VWKP+ +FK
Sbjct: 247 FRKYTTSPQLYQGLNRLVRSVAVPKNKKELLDAQS-DREFQKVKDIPLTFKVWKPHSNFK 305

Query: 298 KSNRYLPDFQIVIHNKNSKKAEFPNLKDFKGIFQRLDHKFPFINDVDIAEEKVT------ 351
           K +  LPDFQ+ ++NKN     FP  K+ + +F  LD+KF F+++++  ++  T      
Sbjct: 306 KRDPGLPDFQVFVYNKNDDLQHFPTYKELRSMFSSLDYKFEFLSEIEDDDDWETNSYVED 365

Query: 352 ---------KSNKTGNVDAQRHLK-------SRRKNNKGDKTLSLYARKMKRIKEGYRSF 395
                    +S K+    ++  +K       ++    K  K    + ++ +R+K GYRSF
Sbjct: 366 IPRKEYIHKRSAKSQTEKSESSMKASFQKKTAQSSTKKKRKAYPPHIQQNRRLKTGYRSF 425

Query: 396 LLAVMDGGLVSIVKITETDFGSENIWYVPS--PKIVQ-WKK 433
           ++A+MD GL+S VK++E DFGSE++WY P+   K+ Q WKK
Sbjct: 426 IIAIMDNGLISFVKMSEADFGSESVWYTPNTQKKVDQRWKK 466


>SPCC613.09 [J] KOG4772 Predicted tRNA-splicing endonuclease subunit
          Length = 384

 Score =  109 bits (273), Expect = 7e-24
 Identities = 114/442 (25%), Positives = 196/442 (43%), Gaps = 76/442 (17%)

Query: 3   DNEETFNDSDVEGDELLQDWSLATKIIGDNKN-VIPLRGEKDYEPDGTGMQGSMLFKAKK 61
           DN  T  ++ V+  E +QDW   + +  D     IP RG+KD+EPDGT  Q S L  ++K
Sbjct: 7   DNPFTNLETTVDVTEEIQDWRFLSNVEKDQGTYTIPKRGQKDFEPDGTNKQHSALDLSRK 66

Query: 62  VMFDVLRQPDRGTIIKNQVKAYYDLDLHVATIKFPKGTFTNTVGSPNSDGTLTLQFHEFV 121
            MFD L   +R    K+ + A ++    ++ ++   G    T+G+ +S   + L   E +
Sbjct: 67  AMFDAL-SVERLISAKHAIIATWNAQNGMSCVEKAHGPLFKTMGTADSQNRMWLLPEETL 125

Query: 122 YLVERGSVSPIIDLGINGEKEEYLLSLQDVYS-FFKSQDELDEFFVYAYLKRMGYIVFPT 180
           YLVERGS+    + G+        +SLQ VYS        L+ + VYA+L+R G+ V  +
Sbjct: 126 YLVERGSMECWSEEGLP-------MSLQAVYSASIPLCGSLENYLVYAHLRRCGFSVIRS 178

Query: 181 NWKYGKQLSVYSRYNKGENRFQNTYLLLNGIFNLFTNISYSWLKDWATNINNIISFHPVK 240
           N    K           E+ ++    ++N    LF  +        A+ I    +F  + 
Sbjct: 179 NLVPVK-----------EDEYRCDSKIMNFKDLLFLGLGK------ASQILQTFNFRKLA 221

Query: 241 FASSGAIFESINKTITFYAPPKSQKQLLESHG---VDLNAPVSSN--YKIAFDVWKPNPS 295
           F  S    +SI    TFY   +    L    G   +  N   SS+  ++I F  +KP+ S
Sbjct: 222 FPFSKRRRQSILLHDTFYTYEEVYHDLQIVRGYVPIACNLITSSDSLFQITFHAYKPSAS 281

Query: 296 FKKSNRYLPDFQIVIHNKNSKKAEFPNLKDFKGIFQRLDHKFPFINDVDIAEEKVTKSNK 355
           FKKS    PDF+I +   +S+    P + +   +F                      S+ 
Sbjct: 282 FKKSALSEPDFRICV--VSSQDTLLPTIFEIDALF----------------------SST 317

Query: 356 TGNVDAQRHLKSRRKNNKGDKTLSLYARKMKRIKEGYRSFLLAVMDGGLVSIVKITETDF 415
               +  +H+                    +R+KEGYR+ ++A++D G++S +++++  F
Sbjct: 318 PLRQNMPQHM-------------------FQRLKEGYRNIIIAIVDYGVISYIRLSDVCF 358

Query: 416 GSENIWYVPSPKIVQWKKPNQK 437
             E ++   S K  + K+ ++K
Sbjct: 359 -EEKVYTDFSKKGSKRKRVSKK 379


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.317    0.136    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 28,682,771
Number of Sequences: 60738
Number of extensions: 1317869
Number of successful extensions: 3045
Number of sequences better than 1.0e-05: 2
Number of HSP's better than  0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 3035
Number of HSP's gapped (non-prelim): 3
length of query: 440
length of database: 30,389,216
effective HSP length: 109
effective length of query: 331
effective length of database: 23,768,774
effective search space: 7867464194
effective search space used: 7867464194
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)